##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938703_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2984961 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00979945801637 33.0 31.0 34.0 30.0 34.0 2 32.230161466096206 34.0 31.0 34.0 30.0 34.0 3 32.31675924744076 34.0 31.0 34.0 30.0 34.0 4 35.94570079810088 37.0 35.0 37.0 35.0 37.0 5 35.85236155514259 37.0 35.0 37.0 35.0 37.0 6 35.88302929251002 37.0 35.0 37.0 35.0 37.0 7 35.815514172546976 37.0 35.0 37.0 33.0 37.0 8 35.78122494732762 37.0 35.0 37.0 33.0 37.0 9 37.436555787496054 39.0 37.0 39.0 34.0 39.0 10-11 37.36018175781861 39.0 37.0 39.0 34.0 39.0 12-13 37.28623606807594 39.0 37.0 39.0 33.0 39.0 14-15 38.51424055456671 40.0 38.0 41.0 34.0 41.0 16-17 38.44121547986724 40.0 38.0 41.0 33.0 41.0 18-19 38.45751686537948 40.0 38.0 41.0 33.5 41.0 20-21 38.53826565908231 40.0 38.0 41.0 34.0 41.0 22-23 38.49670297199863 40.0 38.0 41.0 34.0 41.0 24-25 38.42072073973496 40.0 38.0 41.0 34.0 41.0 26-27 38.26626478536906 40.0 38.0 41.0 33.5 41.0 28-29 38.10274238088873 40.0 37.5 41.0 33.0 41.0 30-31 37.880828928753175 40.0 37.0 41.0 33.0 41.0 32-33 37.668014757981766 40.0 37.0 41.0 32.5 41.0 34-35 37.63464246266534 40.0 37.0 41.0 32.0 41.0 36-37 37.55409199651184 40.0 37.0 41.0 32.0 41.0 38-39 37.45769107201066 40.0 37.0 41.0 32.0 41.0 40-41 37.29700656055473 40.0 36.0 41.0 31.0 41.0 42-43 37.119893861259825 40.0 36.0 41.0 31.0 41.0 44-45 36.87487122947335 40.0 36.0 41.0 31.0 41.0 46-47 36.57718945071644 39.0 35.5 41.0 30.0 41.0 48-49 36.300065059476495 39.0 35.0 41.0 30.0 41.0 50-51 36.05828953879129 39.0 35.0 41.0 29.0 41.0 52-53 35.83583403602258 39.0 35.0 40.5 28.5 41.0 54-55 35.586018041776754 38.5 35.0 40.0 28.0 41.0 56-57 35.326291197774445 38.0 34.5 40.0 28.0 41.0 58-59 35.01813005262045 38.0 34.0 40.0 27.0 41.0 60-61 34.668912927170574 37.5 34.0 40.0 26.0 41.0 62-63 34.29529648796081 37.0 33.5 40.0 26.0 41.0 64-65 33.92760089662813 36.5 33.0 39.5 26.0 41.0 66-67 33.53521570298574 36.0 33.0 39.0 25.0 40.5 68-69 33.08903030893871 35.5 32.5 39.0 23.5 40.0 70-71 32.61932266451723 35.0 32.0 38.0 23.0 40.0 72-73 32.264106633218994 35.0 32.0 37.0 22.0 39.5 74-75 31.838770590302516 35.0 31.0 37.0 21.5 39.0 76-77 29.80729530469577 33.0 28.5 35.0 18.5 37.0 78-79 30.870821092804896 34.0 30.5 36.0 19.5 38.0 80-81 30.928877127707867 34.0 31.0 35.5 19.5 37.0 82-83 30.63588351740609 34.0 31.0 35.0 18.0 37.0 84-85 30.25341654380074 34.0 30.5 35.0 15.5 36.5 86-87 29.725764926241915 34.0 30.0 35.0 7.5 36.0 88-89 29.221698206442227 34.0 29.0 35.0 3.5 35.5 90-91 28.813834418607144 34.0 29.0 35.0 2.0 35.0 92-93 28.530102068335232 34.0 29.0 35.0 2.0 35.0 94-95 28.136598434619415 34.0 29.0 35.0 2.0 35.0 96-97 27.615262477466203 33.0 27.5 35.0 2.0 35.0 98-99 26.872714584880676 33.0 26.5 35.0 2.0 35.0 100-101 23.662976668706893 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.004750218195283651 1101 2 0.05901296684972124 1101 3 0.04953121108702874 1101 4 0.0956790268932437 1101 5 0.08371665544731144 1101 6 0.09820452269759272 1101 7 0.09989219628118917 1101 8 0.1010202900243371 1101 9 0.1407578312438531 1101 10-11 0.15465030022583193 1101 12-13 0.1442970923211746 1101 14-15 0.1312355066570916 1101 16-17 0.09411441774488338 1101 18-19 0.09995703911768317 1101 20-21 0.1973480799278846 1101 22-23 0.1701463683360629 1101 24-25 0.18523351184028058 1101 26-27 0.22124094501614167 1101 28-29 0.1887362710056948 1101 30-31 0.10229650410379065 1101 32-33 0.14538325998674395 1101 34-35 0.10899196268109534 1101 36-37 0.1331244703489176 1101 38-39 0.21700580392624857 1101 40-41 0.22465932510372966 1101 42-43 0.21941618264293794 1101 44-45 0.20131290478065722 1101 46-47 0.20050267948716538 1101 48-49 0.13651887166208354 1101 50-51 0.15814673134362067 1101 52-53 0.23625574082937106 1101 54-55 0.15137811855907302 1101 56-57 -0.024105180471245546 1101 58-59 0.11359838721735116 1101 60-61 0.13928744995749298 1101 62-63 0.16442777204477466 1101 64-65 0.06553072967064821 1101 66-67 -0.04789598966626585 1101 68-69 0.01962830497759427 1101 70-71 -0.012383862960284375 1101 72-73 -0.05166968889064094 1101 74-75 0.1297787466599516 1101 76-77 0.09658745999662699 1101 78-79 0.15633539114405792 1101 80-81 0.17078957805685846 1101 82-83 0.1326590026133907 1101 84-85 0.0999418553658522 1101 86-87 0.25462739099904397 1101 88-89 0.24183022375662944 1101 90-91 0.32264365610012646 1101 92-93 0.25363838976175757 1101 94-95 0.36655442687911943 1101 96-97 0.16852688970802632 1101 98-99 0.35148866708764714 1101 100-101 1.095047066860392 1102 1 0.021023667087298747 1102 2 0.043796442694500115 1102 3 0.051680291385174826 1102 4 0.01433389036670718 1102 5 0.07045355046725632 1102 6 0.04157936613913904 1102 7 -0.04739062046343179 1102 8 0.028968524829984688 1102 9 0.08912575738491313 1102 10-11 0.07433117279641266 1102 12-13 0.1855004874377002 1102 14-15 0.19795151618218654 1102 16-17 0.2958372523555468 1102 18-19 0.1939653517006903 1102 20-21 0.1611683284777996 1102 22-23 0.17893220299094992 1102 24-25 0.0825957752765305 1102 26-27 0.11672019181420978 1102 28-29 0.03472927307627316 1102 30-31 0.09088792371922949 1102 32-33 0.02233712250283304 1102 34-35 0.03261673690234801 1102 36-37 0.07667617725873299 1102 38-39 -0.047810235604153206 1102 40-41 0.03679656879060644 1102 42-43 0.02785566755409974 1102 44-45 -0.10358127151675234 1102 46-47 -0.07868984183634353 1102 48-49 0.016549815675574564 1102 50-51 -0.026793166997720164 1102 52-53 -0.048900602456946274 1102 54-55 -0.03831161610079903 1102 56-57 -0.10679334440381894 1102 58-59 -0.171694032173221 1102 60-61 -0.2303891783627421 1102 62-63 -0.253291110092988 1102 64-65 -0.11457675271807943 1102 66-67 -0.06185177048892143 1102 68-69 -0.15144075666002976 1102 70-71 0.02316693551443194 1102 72-73 0.017516675314269037 1102 74-75 0.028958116718627025 1102 76-77 -0.12263427690194106 1102 78-79 -0.08186269641328892 1102 80-81 -0.12137362058044232 1102 82-83 0.033147824392944614 1102 84-85 0.034589385153044105 1102 86-87 -0.05225791800721069 1102 88-89 0.10764452515420331 1102 90-91 0.09841931945044635 1102 92-93 -0.006983171833898183 1102 94-95 0.010869578663676549 1102 96-97 0.14601687552347897 1102 98-99 0.30265466551147924 1102 100-101 0.7661309065284563 1103 1 0.004501418255813405 1103 2 -0.015362248005352797 1103 3 -0.0056462525000640085 1103 4 0.06143352641274191 1103 5 -0.010674204729625103 1103 6 0.09216254485723141 1103 7 0.08397912850524847 1103 8 0.1284135446623793 1103 9 0.05768069551741206 1103 10-11 0.011869281707035384 1103 12-13 0.15035867149776294 1103 14-15 0.1484603744970201 1103 16-17 0.037774159996246226 1103 18-19 0.09013682924635447 1103 20-21 0.12735558086475152 1103 22-23 0.04612543720705986 1103 24-25 0.04808034080283363 1103 26-27 0.08931984128813042 1103 28-29 0.09663805222498922 1103 30-31 0.07735442932916214 1103 32-33 0.13529607872443705 1103 34-35 0.16889221630689377 1103 36-37 0.08621966432767891 1103 38-39 0.20917045276741675 1103 40-41 0.272757310336587 1103 42-43 0.2970132256413649 1103 44-45 0.21245313655305864 1103 46-47 0.29815576205049155 1103 48-49 0.3874774604135425 1103 50-51 0.38234812583868205 1103 52-53 0.2998809632807067 1103 54-55 0.23736757196776637 1103 56-57 0.18211767778488053 1103 58-59 0.04591336460431705 1103 60-61 0.23065612964365556 1103 62-63 0.156730643074539 1103 64-65 0.22354097360302916 1103 66-67 0.132787936106034 1103 68-69 0.10664965851837849 1103 70-71 0.07008342693752212 1103 72-73 0.10206001642799123 1103 74-75 0.23251679714322648 1103 76-77 0.09253975427643724 1103 78-79 0.17231506771605254 1103 80-81 0.2182354762836205 1103 82-83 0.15050301053257087 1103 84-85 0.1730749770365314 1103 86-87 0.27161930678836654 1103 88-89 0.4289915868418923 1103 90-91 0.4520255228980261 1103 92-93 0.3567931920593459 1103 94-95 0.3084733440750931 1103 96-97 0.5939951620557835 1103 98-99 0.8620472932544168 1103 100-101 1.2146749155333083 1104 1 -0.07720885260539845 1104 2 -0.06664068654794164 1104 3 -0.13442784826278853 1104 4 -0.0851841248039662 1104 5 -0.07298100170623201 1104 6 -0.020492135368201048 1104 7 -0.03493073521593715 1104 8 -0.031009108156496268 1104 9 -0.06405181725283882 1104 10-11 -0.0188064694286183 1104 12-13 -0.07085101504211622 1104 14-15 0.03978390945263044 1104 16-17 0.05527941417602733 1104 18-19 0.04384196844677035 1104 20-21 -0.02098151835678408 1104 22-23 -0.05529236668716209 1104 24-25 -0.0420820911048807 1104 26-27 -0.015381942776862445 1104 28-29 -3.2706991702013966E-4 1104 30-31 -0.07068791747497727 1104 32-33 -0.06406458419755268 1104 34-35 -0.2097265116632414 1104 36-37 -0.24569600255260582 1104 38-39 -0.0337594357403006 1104 40-41 0.002425519485925065 1104 42-43 0.048675831903025824 1104 44-45 0.033650689000616296 1104 46-47 0.049768536068931724 1104 48-49 0.02368082073246569 1104 50-51 0.029627320700427617 1104 52-53 -0.06361998152920734 1104 54-55 -0.11529851194831053 1104 56-57 -0.09274254453996633 1104 58-59 -0.04878486169767626 1104 60-61 -0.02801632997220338 1104 62-63 -0.16164561510124997 1104 64-65 -0.08689605399202804 1104 66-67 -0.15891275706871255 1104 68-69 -0.028267193497534038 1104 70-71 -0.16046898056063696 1104 72-73 -0.25245257004213784 1104 74-75 -0.2515944336071563 1104 76-77 -0.38127110467593184 1104 78-79 -0.22838334943283556 1104 80-81 -0.32950080865785125 1104 82-83 -0.3392340802068752 1104 84-85 -0.19616009213957497 1104 86-87 0.1119375380853711 1104 88-89 0.31394071789951283 1104 90-91 0.18565675016132843 1104 92-93 0.15442704405640129 1104 94-95 0.14702117596018738 1104 96-97 0.09062052961275313 1104 98-99 0.14903795589366453 1104 100-101 0.738100723216192 1105 1 0.03888619530016513 1105 2 0.02087774579517543 1105 3 -0.02546954426065895 1105 4 -0.03864732125013148 1105 5 0.002284553709358761 1105 6 -0.03836432976180504 1105 7 0.021104514093082116 1105 8 -0.020251659266882882 1105 9 0.08237601317701149 1105 10-11 -0.019630251597227755 1105 12-13 -0.06111660750763548 1105 14-15 -0.03076426287402967 1105 16-17 -0.14482196371108813 1105 18-19 -0.04867612118442821 1105 20-21 0.01703183166137734 1105 22-23 -0.010824338929353416 1105 24-25 -0.022418300748235254 1105 26-27 -0.05964943775659748 1105 28-29 -0.021817306332657438 1105 30-31 -0.03064006285151777 1105 32-33 -0.08565798152847037 1105 34-35 -0.0399272343222421 1105 36-37 -0.1433308504255706 1105 38-39 -0.12714067309700994 1105 40-41 -0.07687014270757686 1105 42-43 -0.08761444137730479 1105 44-45 -0.11713676107459747 1105 46-47 -0.0478788856260266 1105 48-49 -0.0879477416188692 1105 50-51 -0.1068323402556075 1105 52-53 -0.237627205340452 1105 54-55 -0.19760147112669557 1105 56-57 -0.18700557037117704 1105 58-59 -0.12200942876917509 1105 60-61 -0.1658976664374876 1105 62-63 -0.2785700026658091 1105 64-65 -0.13422213696164675 1105 66-67 -0.12248815461452267 1105 68-69 -0.11438073858359843 1105 70-71 -0.15167436780657084 1105 72-73 -0.3519888437818892 1105 74-75 -0.3005437668935613 1105 76-77 -0.2543810948206513 1105 78-79 -0.43686414922980177 1105 80-81 -0.21563605111262873 1105 82-83 -0.2193739483461563 1105 84-85 -0.13605757033569077 1105 86-87 -0.06725922393548345 1105 88-89 -0.02752283793103416 1105 90-91 0.11447317143392155 1105 92-93 -0.03904841591854691 1105 94-95 -0.025468621413715198 1105 96-97 -0.12850726395241452 1105 98-99 0.020110083782633126 1105 100-101 0.6665726958659306 1106 1 -0.158642596215131 1106 2 -0.1363845511417381 1106 3 -0.14636884042206333 1106 4 -0.12079018824938714 1106 5 -0.14045641510593043 1106 6 -0.16660765865800897 1106 7 -0.1167355266046286 1106 8 -0.1334033033201365 1106 9 -0.09667966007850026 1106 10-11 -0.20322026078733302 1106 12-13 -0.09455216041788361 1106 14-15 -0.09745598230636432 1106 16-17 -0.2150771477477278 1106 18-19 -0.303135022842973 1106 20-21 -0.22737614266153372 1106 22-23 -0.3070365723450763 1106 24-25 -0.2611623798475122 1106 26-27 -0.19641718481994275 1106 28-29 -0.17727493020815643 1106 30-31 -0.1182244319495851 1106 32-33 -0.20165818943269187 1106 34-35 -0.15578473805486226 1106 36-37 -0.06320585031058101 1106 38-39 -0.08408770995585257 1106 40-41 -0.04448859981904718 1106 42-43 -0.08552493492904745 1106 44-45 -0.03374554879979286 1106 46-47 -0.05784000477630258 1106 48-49 -0.15550925505123558 1106 50-51 -0.29476752473381396 1106 52-53 -0.2269777484212412 1106 54-55 -0.31784140732033705 1106 56-57 -0.41154075264154955 1106 58-59 -0.2964648760898285 1106 60-61 -0.1610763122818426 1106 62-63 -0.11522271245819127 1106 64-65 -0.22679160779020435 1106 66-67 -0.17441407447572743 1106 68-69 -0.051953815538517745 1106 70-71 0.0037198958767064028 1106 72-73 0.022909388940206554 1106 74-75 0.006043669251784678 1106 76-77 -0.0584342382681271 1106 78-79 0.06319438132889843 1106 80-81 0.03225742373049556 1106 82-83 0.2379796976591848 1106 84-85 0.10612555280892622 1106 86-87 0.19009205324960732 1106 88-89 0.35696773692414396 1106 90-91 0.3741855644575338 1106 92-93 0.4061262742259615 1106 94-95 0.3785083748118119 1106 96-97 0.29682119712396116 1106 98-99 0.3616851100696401 1106 100-101 0.8001488601065887 1107 1 -0.028173069961681563 1107 2 -0.042032728937293484 1107 3 -0.08144670029172829 1107 4 -0.033714935788538014 1107 5 0.040637852539724406 1107 6 -0.02821682073663112 1107 7 -0.017829225427810513 1107 8 -0.0011440505510265098 1107 9 -0.035726662246446494 1107 10-11 0.041945436281672244 1107 12-13 -0.05300449538827223 1107 14-15 0.010092980005481422 1107 16-17 0.07189849197113318 1107 18-19 -0.018714611657721036 1107 20-21 -0.012579372839219616 1107 22-23 -0.06714624498773958 1107 24-25 -0.012261835704592272 1107 26-27 0.0503828447221224 1107 28-29 -0.06191566835623519 1107 30-31 -0.047072882963419715 1107 32-33 0.02083259243214286 1107 34-35 -0.007948580028639185 1107 36-37 0.030544579746731415 1107 38-39 -0.06590259310411994 1107 40-41 -0.10040293148475143 1107 42-43 -0.08139397639896373 1107 44-45 -0.07081396621639868 1107 46-47 -0.3215581449837046 1107 48-49 -0.4315459569451363 1107 50-51 -0.40483455862737827 1107 52-53 -0.22299839942939315 1107 54-55 -0.3222766170644462 1107 56-57 -0.3860077338731642 1107 58-59 -0.3008235929731242 1107 60-61 -0.39291504927575716 1107 62-63 -0.374810696909158 1107 64-65 -0.30805902224434334 1107 66-67 -0.35953299048357934 1107 68-69 -0.24833862761150272 1107 70-71 -0.42282757648724356 1107 72-73 -0.3338177625161123 1107 74-75 -0.33869119066650555 1107 76-77 -0.3473844718064498 1107 78-79 -0.40511222958093285 1107 80-81 -0.2495435370290089 1107 82-83 -0.17428402311870528 1107 84-85 -0.2693663180757149 1107 86-87 -0.3703213660065039 1107 88-89 -0.2434294296059747 1107 90-91 -0.13301302155988992 1107 92-93 -0.06142882586385667 1107 94-95 0.09022341126690847 1107 96-97 0.0488582926063188 1107 98-99 -0.06434721097376439 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-2.35626113472388 2316 94-95 -2.3301133671283054 2316 96-97 -2.392788513829185 2316 98-99 -2.8998424582828015 2316 100-101 -3.401168724441412 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 34.0 5 85.0 6 194.0 7 508.0 8 1156.0 9 2057.0 10 3331.0 11 4970.0 12 7001.0 13 9328.0 14 11466.0 15 15035.0 16 19154.0 17 25034.0 18 24018.0 19 17190.0 20 16362.0 21 17406.0 22 18236.0 23 20214.0 24 23747.0 25 28310.0 26 34756.0 27 42666.0 28 51819.0 29 63477.0 30 77256.0 31 93899.0 32 114773.0 33 144869.0 34 191289.0 35 268790.0 36 405365.0 37 633313.0 38 540875.0 39 56955.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11854775568847 19.297541118133413 14.499035442445377 23.084875683732744 2 16.139741859273872 20.59219534191569 39.08339170930542 24.184671089505024 3 19.71235135065416 24.360787293368322 29.57874491492519 26.348116441052326 4 13.493509630444084 16.711240113354915 35.72951204387595 34.06573821232505 5 14.01147284671391 37.34491003400044 33.62878107955179 15.014836039733853 6 32.76816682027001 35.46769957798443 16.93117598521388 14.832957616531672 7 29.13515452965717 32.51258559157054 20.10538831160608 18.246871567166203 8 26.723129715932636 35.06558377144626 19.142394155233518 19.068892357387586 9 26.686311814459216 16.674924731009884 19.972823765536635 36.66593968899426 10-11 25.550467828557892 26.484181870382894 26.542876104578923 21.42247419648029 12-13 26.276440462706212 23.744397330484386 27.32265178674026 22.65651042006914 14-15 23.056328784635756 25.152389129893667 25.776049705238634 26.015232380231947 16-17 22.619622536705172 28.4840410874805 26.3641204670375 22.532215908776827 18-19 22.368699422386506 26.811681719101582 28.809718875111834 22.009899983400082 20-21 23.131009606324774 25.906540471808587 28.909913548269735 22.052536373596904 22-23 22.78126916901092 25.96645316303965 28.789253862948293 22.463023805001136 24-25 22.78453554334546 26.104846930998427 28.854866110478493 22.25575141517762 26-27 22.55833921840054 26.367323337429426 28.769033781402477 22.30530366276756 28-29 22.24632080620149 26.44186975977241 28.959038325793873 22.352771108232236 30-31 22.39798107914978 26.147410301173114 28.934381387227504 22.520227232449603 32-33 22.266823586639823 26.346257120277283 28.802453365387354 22.584465927695536 34-35 22.893280002063833 26.293592357526556 28.494755239190813 22.3183724012188 36-37 22.862079849460507 26.10133605140752 28.534853471274257 22.501730627857714 38-39 22.562087197990508 26.358617625684634 28.721956769894323 22.35733840643054 40-41 22.680496821492298 26.152481259636538 28.76092140874195 22.406100510129214 42-43 22.57284127063097 26.301336727793416 28.397529736284422 22.728292265291195 44-45 22.603633951499294 26.43876438278605 28.252490447769695 22.70511121794496 46-47 22.739634884197745 26.61967706963948 27.84063270122174 22.800055344941033 48-49 22.925181576972555 26.682685865137223 27.6507557890223 22.741376768867923 50-51 22.6902894239478 26.7948018498824 27.68128339988673 22.833625326283066 52-53 23.007436278061924 26.79748579629684 27.469571629244065 22.72550629639717 54-55 22.6050022094091 26.61951027165849 27.691149063589105 23.084338455343303 56-57 22.73808602524455 27.07909751584694 27.41578533186866 22.76703112703985 58-59 22.88976606791242 26.617520073636136 27.500249166861394 22.992464691590055 60-61 22.86154304351664 26.559860446035987 27.90380773339216 22.674788777055205 62-63 22.724132744112904 26.60520522713697 27.79386397343215 22.876798055317977 64-65 22.47590504532555 26.606729535159758 27.723863058847336 23.19350236066736 66-67 22.467278986589978 26.877982480002892 27.409217362405574 23.245521171001553 68-69 22.3596621707639 26.7731009668536 27.64559756645025 23.22163929593225 70-71 22.387012144246153 26.702634232204204 27.539241950724502 23.37111167282514 72-73 22.48364383990277 26.59515484456916 27.505501746924 23.41569956860408 74-75 22.353089370346883 26.897302845832826 27.223571765259248 23.526036018561047 76-77 22.395679636654346 26.75824529587561 27.443894564301775 23.402180503168278 78-79 22.390866562574463 26.78003199176378 27.42538019962512 23.40372124603664 80-81 22.24005606773422 27.033502280264297 27.326722191680226 23.399719460321258 82-83 22.30694136372301 26.902864057520347 27.279753403813316 23.510441174943324 84-85 22.32251945670312 26.666445558250174 27.161242642701193 23.84979234234551 86-87 22.30541594250552 26.566989659314334 27.41826764996007 23.709326748220075 88-89 22.255581604443435 26.648583696757335 27.190266118799734 23.905568579999496 90-91 22.223775138342987 26.690579708440744 27.317310150684754 23.768335002531515 92-93 22.231077725973638 26.530128869355412 27.32498012536847 23.913813279302477 94-95 22.014047089408233 26.43249825793311 27.318155017152655 24.235299635506003 96-97 22.057105603724807 26.30220294335504 27.336688821059973 24.30400263186018 98-99 21.937740131221343 26.270818351959235 27.16997718392211 24.621464332897315 100-101 21.967912636231596 25.92294429594922 27.66173709427428 24.447405973544903 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1767.0 1 1158.5 2 754.0 3 968.5 4 807.0 5 776.0 6 1386.0 7 2731.0 8 4174.0 9 4727.5 10 4002.0 11 3168.5 12 3123.5 13 3288.5 14 3503.5 15 3726.0 16 3946.0 17 4218.5 18 4444.0 19 4722.5 20 5159.0 21 5850.0 22 6968.0 23 8222.5 24 9685.0 25 11795.0 26 14781.5 27 18297.5 28 21620.5 29 25498.5 30 30096.5 31 35253.0 32 40914.5 33 45819.0 34 50955.5 35 56082.0 36 61827.0 37 68013.0 38 72264.0 39 74920.5 40 77395.0 41 82912.5 42 88704.0 43 93394.0 44 98268.0 45 102291.0 46 105087.0 47 109602.5 48 122107.5 49 125069.0 50 117874.5 51 114559.0 52 110293.0 53 109516.5 54 109065.5 55 102215.0 56 93258.5 57 84451.0 58 76661.0 59 68394.5 60 58289.5 61 47359.5 62 38386.0 63 30908.0 64 24189.5 65 18934.0 66 13935.5 67 11316.5 68 8446.5 69 5272.0 70 3850.0 71 2983.0 72 2398.0 73 1958.0 74 1562.5 75 1268.5 76 890.5 77 504.5 78 324.0 79 215.5 80 123.5 81 70.5 82 49.5 83 34.0 84 17.5 85 9.5 86 6.0 87 4.5 88 2.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3878442632918822 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.340051009041659E-4 16-17 0.002060328426401551 18-19 2.177582889692696E-4 20-21 1.675063761302074E-5 22-23 0.0 24-25 0.0 26-27 1.675063761302074E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007387031187342147 36-37 0.016583131236890532 38-39 0.010988418274141605 40-41 0.0076047894763114155 42-43 0.0032663743345390447 44-45 0.0018760714126583229 46-47 0.0015075573851718665 48-49 3.350127522604148E-5 50-51 4.857684907776015E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.5226721555155996E-4 60-61 7.537786925859332E-4 62-63 0.0 64-65 0.0 66-67 9.71536981555203E-4 68-69 7.035267797468711E-4 70-71 0.0016583131236890534 72-73 0.0 74-75 0.0 76-77 0.09705319432984216 78-79 0.0357793619414123 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1507892397924127 88-89 0.3278434793620419 90-91 0.28415781646728383 92-93 0.0 94-95 3.350127522604148E-5 96-97 0.0 98-99 1.5075573851718667E-4 100-101 5.527710412296845E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2984961.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.78739008740425 #Duplication Level Percentage of deduplicated Percentage of total 1 74.25541422438037 37.712386883153926 2 14.273357566434965 14.498131571670712 3 4.838510657528667 7.372059846179137 4 2.1863328639779023 4.441525600950298 5 1.1832199833776087 3.004632742750529 6 0.7155254493632052 2.180380206856459 7 0.4587518377077288 1.6309165974483193 8 0.3309943875494682 1.344827286177304 9 0.24519567008409227 1.1207563329872943 >10 1.3178766868106215 12.353613599282205 >50 0.11776962055921722 4.150679201060222 >100 0.07129645519292836 6.765093968570951 >500 0.004497845957130692 1.6341419091566116 >1k 0.0010583166957424156 0.8628753022317935 >5k 1.3228958696780195E-4 0.5483420368422318 >10k+ 6.614479348390098E-5 0.37963691468198624 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11301 0.3785979113294948 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9937 0.3329021719211742 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6386 0.2139391435935009 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.350127522604148E-5 0.0 10-11 0.0 0.0 0.0 3.350127522604148E-5 0.0 12-13 0.0 0.0 0.0 3.350127522604148E-5 0.0 14-15 0.0 0.0 0.0 3.350127522604148E-5 0.0 16-17 0.0 0.0 0.0 6.700255045208296E-5 0.0 18-19 0.0 0.0 0.0 6.700255045208296E-5 0.0 20-21 0.0 0.0 0.0 1.340051009041659E-4 0.0 22-23 0.0 0.0 0.0 3.6851402748645627E-4 0.0 24-25 0.0 0.0 0.0 7.035267797468711E-4 0.0 26-27 0.0 0.0 0.0 0.0011892952705244726 0.0 28-29 0.0 0.0 0.0 0.004003402389511957 0.0 30-31 0.0 0.0 0.0 0.012512726296926492 0.0 32-33 0.0 0.0 0.0 0.029146109446656084 0.0 34-35 1.675063761302074E-5 0.0 0.0 0.05485833818264292 0.0 36-37 3.350127522604148E-5 0.0 0.0 0.09750546154539372 0.0 38-39 3.350127522604148E-5 0.0 0.0 0.15931531433744026 0.0 40-41 3.350127522604148E-5 0.0 0.0 0.22723914985823934 0.0 42-43 3.350127522604148E-5 0.0 0.0 0.2954477462184598 0.0 44-45 3.350127522604148E-5 0.0 0.0 0.38216579714106813 0.0 46-47 3.350127522604148E-5 0.0 0.0 0.4898891476304046 0.0 48-49 3.350127522604148E-5 0.0 0.0 0.6081151479031048 0.0 50-51 3.350127522604148E-5 0.0 0.0 0.7314835939230027 0.0 52-53 3.350127522604148E-5 0.0 0.0 0.85362924339715 0.0 54-55 3.350127522604148E-5 0.0 0.0 0.9950213754886579 0.0 56-57 3.350127522604148E-5 0.0 0.0 1.1636165430637115 0.0 58-59 3.350127522604148E-5 0.0 0.0 1.3546575650402133 0.0 60-61 3.350127522604148E-5 0.0 0.0 1.5607574102308206 0.0 62-63 3.350127522604148E-5 0.0 0.0 1.7654837031371597 0.0 64-65 3.350127522604148E-5 0.0 0.0 1.9757209558181832 0.0 66-67 3.350127522604148E-5 0.0 0.0 2.2210842955737107 0.0 68-69 3.350127522604148E-5 0.0 0.0 2.5049406005639607 0.0 70-71 6.700255045208296E-5 0.0 0.0 2.8223986846059295 3.350127522604148E-5 72-73 1.0050382567812443E-4 0.0 0.0 3.1351665901162526 3.350127522604148E-5 74-75 1.0050382567812443E-4 0.0 0.0 3.447029961195473 3.350127522604148E-5 76-77 1.0050382567812443E-4 0.0 0.0 3.7900327675972987 3.350127522604148E-5 78-79 1.0050382567812443E-4 0.0 0.0 4.182165194118114 3.350127522604148E-5 80-81 1.0050382567812443E-4 0.0 0.0 4.614348395171662 3.350127522604148E-5 82-83 1.0050382567812443E-4 0.0 0.0 5.048457919550708 3.350127522604148E-5 84-85 1.0050382567812443E-4 0.0 0.0 5.482332935003171 3.350127522604148E-5 86-87 1.0050382567812443E-4 0.0 0.0 5.93769901851314 3.350127522604148E-5 88-89 1.0050382567812443E-4 0.0 0.0 6.427219652116058 3.350127522604148E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11480 0.0 28.964085 1 GTATCAA 16160 0.0 28.034027 1 GTACATG 13865 0.0 23.329002 1 ATCAACG 19405 0.0 23.206793 3 TCAACGC 19525 0.0 23.064165 4 TACCTGG 2250 0.0 22.988285 2 TATCAAC 19705 0.0 22.949806 2 CAACGCA 19915 0.0 22.636322 5 TACATGG 14225 0.0 22.617262 2 AACGCAG 20290 0.0 22.264732 6 ACATGGG 13985 0.0 22.19105 3 GAGTACT 10890 0.0 20.829418 12-13 ACGCAGA 22605 0.0 19.900612 7 CGCAGAG 22825 0.0 19.708797 8 GTACTTT 11505 0.0 19.633488 14-15 AGTACTT 11445 0.0 19.487637 12-13 GCAGAGT 23795 0.0 18.74583 9 CATGGGG 6590 0.0 18.649918 4 GTACCTG 2950 0.0 18.085993 1 CATGGGA 7715 0.0 18.083147 4 >>END_MODULE