FastQCFastQC Report
Thu 26 May 2016
SRR938702_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938702_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993214
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115580.38614011560817235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90810.30338625971948546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58610.19580958795462003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA158600.030.1474671
GGTATCA108100.027.7434021
GTACATG137950.027.3905931
TACATGG139300.026.4970912
ATCAACG189650.025.0663953
TATCAAC190500.024.9790362
TCAACGC190650.024.95984
ACATGGG142250.024.8807533
CAACGCA192850.024.6750625
AACGCAG196800.024.1773786
TACCTGG25200.024.0978972
GAGTACT99850.022.41280212-13
GTACCTG29500.022.3948841
ACGCAGA218550.021.6630887
CGCAGAG220400.021.481258
CATGGGG67250.021.3054964
GTACTTT108100.020.8125914-15
AGTACTT105500.020.24522212-13
GCAGAGT232350.019.9262669
GTATACG1456.1242166E-419.6670531
Produced by FastQC (version 0.11.5)