##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938702_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2993214 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.410742432716138 33.0 31.0 34.0 30.0 34.0 2 31.470661302532996 33.0 31.0 34.0 28.0 34.0 3 31.481424315134166 33.0 31.0 34.0 28.0 34.0 4 35.027849328514435 37.0 35.0 37.0 32.0 37.0 5 35.05398878930808 37.0 35.0 37.0 32.0 37.0 6 35.10928921219799 37.0 35.0 37.0 32.0 37.0 7 35.00610781587952 37.0 35.0 37.0 32.0 37.0 8 34.972179403143244 37.0 35.0 37.0 32.0 37.0 9 36.519488750219665 39.0 37.0 39.0 32.0 39.0 10-11 36.39429289051835 39.0 37.0 39.0 32.0 39.0 12-13 36.26865653441418 39.0 37.0 39.0 32.0 39.0 14-15 37.40675507999094 40.0 37.0 41.0 32.0 41.0 16-17 37.28058418141836 40.0 37.0 41.0 31.5 41.0 18-19 37.206754010906 40.0 37.0 41.0 31.0 41.0 20-21 37.09386131429294 40.0 37.0 41.0 31.0 41.0 22-23 37.11034059041552 40.0 37.0 41.0 31.0 41.0 24-25 37.04240007563776 40.0 37.0 41.0 30.5 41.0 26-27 36.90553281522804 40.0 36.5 41.0 30.0 41.0 28-29 36.722941794338794 40.0 36.0 41.0 30.0 41.0 30-31 36.487151436549475 39.0 36.0 41.0 30.0 41.0 32-33 36.301446037603725 39.0 35.5 41.0 29.5 41.0 34-35 36.004348670024925 39.0 35.0 41.0 28.0 41.0 36-37 35.78781771032743 39.0 35.0 41.0 27.0 41.0 38-39 35.61500263596255 39.0 35.0 40.5 27.0 41.0 40-41 35.497118147917256 39.0 35.0 40.5 26.0 41.0 42-43 35.48126395239365 39.0 35.0 41.0 26.0 41.0 44-45 35.30734788758839 39.0 35.0 41.0 25.5 41.0 46-47 34.997913948017086 39.0 34.5 41.0 24.0 41.0 48-49 34.7107264966688 39.0 34.0 40.5 23.0 41.0 50-51 33.57377554695388 37.5 32.5 39.5 21.5 40.5 52-53 33.63506635342478 37.5 33.0 39.5 22.0 40.5 54-55 34.112790799455034 38.0 34.0 40.0 22.0 41.0 56-57 33.998655959781026 38.0 34.0 40.0 21.0 41.0 58-59 33.78262914044903 38.0 33.0 40.0 20.0 41.0 60-61 33.467175250416446 37.0 33.0 40.0 19.5 41.0 62-63 33.057345548965095 37.0 32.5 40.0 17.5 41.0 64-65 32.64985112992255 36.0 32.0 39.5 13.5 41.0 66-67 32.257887174121194 36.0 31.5 39.0 12.0 41.0 68-69 31.831492836796834 35.0 31.0 39.0 8.0 40.0 70-71 31.408811398049053 35.0 31.0 38.0 7.0 40.0 72-73 30.987545828664437 35.0 30.5 37.0 4.5 39.5 74-75 30.516096410079598 35.0 30.0 37.0 2.0 39.0 76-77 30.058901401637172 34.5 30.0 36.0 2.0 39.0 78-79 29.625693819419528 34.0 29.0 36.0 2.0 38.5 80-81 29.16408833447926 34.0 29.0 35.0 2.0 37.0 82-83 28.756846152663993 34.0 29.0 35.0 2.0 37.0 84-85 28.409752526882475 34.0 28.5 35.0 2.0 36.0 86-87 27.98058842434921 34.0 27.0 35.0 2.0 36.0 88-89 27.531319511401456 34.0 26.5 35.0 2.0 35.5 90-91 27.079076036661597 33.0 25.5 35.0 2.0 35.0 92-93 26.609278354304102 33.0 25.0 35.0 2.0 35.0 94-95 26.083671097355552 33.0 24.0 35.0 2.0 35.0 96-97 25.465087193899265 33.0 21.5 35.0 2.0 35.0 98-99 24.66831957220566 32.0 10.0 35.0 2.0 35.0 100-101 22.190897643803616 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06545857040561387 1101 2 0.10669048168272965 1101 3 0.2656318809868985 1101 4 0.08354635802225374 1101 5 -0.03816634576568134 1101 6 0.1909098189018863 1101 7 0.21318567008012224 1101 8 0.20471252494376557 1101 9 0.2951823204177657 1101 10-11 0.22936187853227352 1101 12-13 0.15229130017031878 1101 14-15 0.1792911948911211 1101 16-17 0.4013633379290624 1101 18-19 0.4523611650418857 1101 20-21 0.5186121411056774 1101 22-23 0.5094945304532033 1101 24-25 0.4328906655762452 1101 26-27 0.43108797514528163 1101 28-29 0.5476354905327128 1101 30-31 0.5851427548505725 1101 32-33 0.44675700158133225 1101 34-35 0.4288893747967819 1101 36-37 0.46405773067638023 1101 38-39 0.4165225113515447 1101 40-41 0.7158141440813139 1101 42-43 0.6805421445492783 1101 44-45 0.6474811427139429 1101 46-47 0.5570229369570256 1101 48-49 0.5646319718047792 1101 50-51 0.6465094960486866 1101 52-53 0.686619165371738 1101 54-55 0.5904583182940186 1101 56-57 0.6247283555222651 1101 58-59 0.5591273266708328 1101 60-61 0.5225756026925268 1101 62-63 0.4624350610638075 1101 64-65 0.46453493214274744 1101 66-67 0.4600730751249458 1101 68-69 0.4531145697804675 1101 70-71 0.39317533954896433 1101 72-73 0.3329830460513996 1101 74-75 0.37136140268248496 1101 76-77 0.18920848003787683 1101 78-79 0.2391070792173906 1101 80-81 0.16143143430482 1101 82-83 0.11864778612557814 1101 84-85 0.14389838025962476 1101 86-87 0.0439161538999393 1101 88-89 0.14881172993526448 1101 90-91 0.1294464565952893 1101 92-93 -1.8699874595995425E-4 1101 94-95 0.03160131911788966 1101 96-97 -0.02770171148563705 1101 98-99 -0.11337007645624197 1101 100-101 -0.07211108412742817 1102 1 0.10741886658843214 1102 2 0.08217788844167728 1102 3 0.11423668483873328 1102 4 0.23787192202801322 1102 5 0.2889948404246141 1102 6 0.31017993981888736 1102 7 0.20006170735830153 1102 8 0.19748714149280033 1102 9 0.3552354837742655 1102 10-11 0.3549138017144102 1102 12-13 0.3946484977410307 1102 14-15 0.4337656254140043 1102 16-17 0.54665204322351 1102 18-19 0.5081484933780516 1102 20-21 0.5218505007927163 1102 22-23 0.34105332018893364 1102 24-25 0.4015067313054814 1102 26-27 0.4455433015117549 1102 28-29 0.5232024599478322 1102 30-31 0.5981632177274179 1102 32-33 0.6041745664625182 1102 34-35 0.5704712354230708 1102 36-37 0.5461128181269075 1102 38-39 0.5417796651514024 1102 40-41 0.6367277239904823 1102 42-43 0.6486698916266604 1102 44-45 0.5930235822098453 1102 46-47 0.6632265444053616 1102 48-49 0.6662104584273081 1102 50-51 0.5225289923190459 1102 52-53 0.5339867585215998 1102 54-55 0.4166957569692187 1102 56-57 0.4396350688778483 1102 58-59 0.3481613585141332 1102 60-61 0.4007202653833417 1102 62-63 0.27349951365449954 1102 64-65 0.30061944637738947 1102 66-67 0.24288229800981043 1102 68-69 0.3349115915382299 1102 70-71 0.33575663480164764 1102 72-73 0.2031021392650949 1102 74-75 0.06550431453891647 1102 76-77 0.08984885683202748 1102 78-79 0.19596291806767852 1102 80-81 0.19598191669616583 1102 82-83 0.10209305431328275 1102 84-85 6.231345044831471E-4 1102 86-87 -0.011418647580978103 1102 88-89 0.03261901960716429 1102 90-91 -0.09740719371201934 1102 92-93 -0.13142525812477857 1102 94-95 -0.16967419108619097 1102 96-97 -0.2539715223590484 1102 98-99 -0.39134330328048605 1102 100-101 -0.018302107453649796 1103 1 0.12555246711923118 1103 2 0.24319043377624183 1103 3 0.3426846739350644 1103 4 0.30445753939621056 1103 5 0.30869098871740874 1103 6 0.27828166905039353 1103 7 0.20691471099225822 1103 8 0.2603223745174006 1103 9 0.3534893051835084 1103 10-11 0.3976837143088332 1103 12-13 0.4219329596510164 1103 14-15 0.5009161829145015 1103 16-17 0.49709486997850405 1103 18-19 0.4549960218014917 1103 20-21 0.39048624285905476 1103 22-23 0.4349304794806983 1103 24-25 0.47576697102800125 1103 26-27 0.43255630895767894 1103 28-29 0.45983487732969053 1103 30-31 0.5180952316159306 1103 32-33 0.4767081369650654 1103 34-35 0.4340656706672448 1103 36-37 0.6228270130372167 1103 38-39 0.5858431973724336 1103 40-41 0.6670309688393843 1103 42-43 0.7723621119728676 1103 44-45 0.6652616351926213 1103 46-47 0.6549917019277984 1103 48-49 0.6269271715748275 1103 50-51 0.5311794203564375 1103 52-53 0.4415798820527712 1103 54-55 0.5604425091534608 1103 56-57 0.4605815700475162 1103 58-59 0.495320677250497 1103 60-61 0.43101193497192014 1103 62-63 0.32103427539743024 1103 64-65 0.2815034096746061 1103 66-67 0.2335161143124438 1103 68-69 0.1593280410844784 1103 70-71 0.25461540853430265 1103 72-73 0.2156600605191592 1103 74-75 0.1004598026058332 1103 76-77 0.01200478165711516 1103 78-79 0.1291353440444496 1103 80-81 0.13722668198135324 1103 82-83 0.11945740574807573 1103 84-85 0.039320915470892004 1103 86-87 0.10176323639490548 1103 88-89 0.16095123362406483 1103 90-91 0.1881462745506397 1103 92-93 0.20208855936460424 1103 94-95 0.26254411877296135 1103 96-97 0.2929651468069778 1103 98-99 0.4339759041895377 1103 100-101 0.8091215497711985 1104 1 0.14445482936192633 1104 2 0.1982251248342628 1104 3 0.17604792490113397 1104 4 0.20538753124253617 1104 5 0.22463427381637047 1104 6 0.3608478606965022 1104 7 0.33909081243000827 1104 8 0.24650623310612474 1104 9 0.45778909415255953 1104 10-11 0.3334761207671164 1104 12-13 0.424234831244533 1104 14-15 0.5175107570169075 1104 16-17 0.5434213195771136 1104 18-19 0.5101480614249425 1104 20-21 0.5803665366055597 1104 22-23 0.5318266590436593 1104 24-25 0.47892339291485087 1104 26-27 0.330121236918707 1104 28-29 0.42670489459261063 1104 30-31 0.42676752726287503 1104 32-33 0.49516954902374977 1104 34-35 0.6199432530724636 1104 36-37 0.6134838926191861 1104 38-39 0.5734126442355887 1104 40-41 0.6230317315157521 1104 42-43 0.5975706869915527 1104 44-45 0.6958402466750968 1104 46-47 0.7393127636733254 1104 48-49 0.8291357626686633 1104 50-51 0.7803666207894011 1104 52-53 0.7658686014977221 1104 54-55 0.7197822582561599 1104 56-57 0.681104887493241 1104 58-59 0.6083840999527155 1104 60-61 0.746992706418169 1104 62-63 0.760354841492628 1104 64-65 0.7849870277612823 1104 66-67 0.7205119910375188 1104 68-69 0.5917030278001647 1104 70-71 0.7191226026086461 1104 72-73 0.5604665827417108 1104 74-75 0.3513323258053944 1104 76-77 0.31348744595180733 1104 78-79 0.33787312358453647 1104 80-81 0.3824270229530562 1104 82-83 0.30571989859240034 1104 84-85 0.40326367098310456 1104 86-87 0.4782338889833184 1104 88-89 0.6173837925818937 1104 90-91 0.4697684309243009 1104 92-93 0.4927817866376998 1104 94-95 0.5273131591135432 1104 96-97 0.6441138722711734 1104 98-99 0.6798887983320192 1104 100-101 0.9880982059800942 1105 1 -0.018360275240308255 1105 2 0.06385941777266169 1105 3 0.007909738296216773 1105 4 0.1247849415470128 1105 5 0.1576005417397326 1105 6 0.15891927390737948 1105 7 0.10653997754535993 1105 8 0.1925364310151636 1105 9 0.24312707743966655 1105 10-11 0.22950919683968607 1105 12-13 0.20682737234796633 1105 14-15 0.1958896123984175 1105 16-17 0.26209066311688645 1105 18-19 0.2827903024390963 1105 20-21 0.36426250565606466 1105 22-23 0.2948417688591718 1105 24-25 0.2686410179194709 1105 26-27 0.330437943517623 1105 28-29 0.4317559086836624 1105 30-31 0.4066104446997585 1105 32-33 0.29544639577294163 1105 34-35 0.2981503418263358 1105 36-37 0.4322955151865884 1105 38-39 0.4259242492081867 1105 40-41 0.5002666526540693 1105 42-43 0.38091030160003214 1105 44-45 0.3917146436183785 1105 46-47 0.39455775512573155 1105 48-49 0.33644923051902964 1105 50-51 0.4266168081415529 1105 52-53 0.46321053601460704 1105 54-55 0.3119032584234134 1105 56-57 0.20734146601932224 1105 58-59 0.3371244635353605 1105 60-61 0.4060692938068229 1105 62-63 0.3565922671762749 1105 64-65 0.3716350642020956 1105 66-67 0.27947719892781464 1105 68-69 0.18186133765564705 1105 70-71 0.2162646405222084 1105 72-73 0.31382556250333593 1105 74-75 0.2767247878407879 1105 76-77 0.2671920225576585 1105 78-79 0.26931787479173863 1105 80-81 0.21015106897760916 1105 82-83 0.2115188007630806 1105 84-85 0.28909957656196283 1105 86-87 0.3103303541640585 1105 88-89 0.2947528470019911 1105 90-91 0.3541987065980621 1105 92-93 0.4316914622632382 1105 94-95 0.4711292346886857 1105 96-97 0.501056708685045 1105 98-99 0.5436074290647248 1105 100-101 0.9546889180920566 1106 1 -0.034983508128334506 1106 2 0.008778608934914445 1106 3 0.06301349987280958 1106 4 0.0014404864413677387 1106 5 0.059846923146125164 1106 6 -0.0742461760370432 1106 7 -0.015196842578006908 1106 8 -0.07625711957963688 1106 9 -0.06482290235878452 1106 10-11 -0.0764457288023408 1106 12-13 0.017723949298073194 1106 14-15 0.15126608185843793 1106 16-17 0.17435109390520864 1106 18-19 0.14380324139057876 1106 20-21 0.1545103166303079 1106 22-23 0.10390621417584356 1106 24-25 -0.039511658668580196 1106 26-27 -0.10644668857565165 1106 28-29 -0.20927184998138415 1106 30-31 -0.4003281472911979 1106 32-33 -0.2338384167980294 1106 34-35 -0.14504115790457206 1106 36-37 0.04767673240492343 1106 38-39 0.06293747952319961 1106 40-41 0.19037507028281908 1106 42-43 0.12406726741564711 1106 44-45 0.06605767387249983 1106 46-47 0.05075669772763547 1106 48-49 -0.0025309132657298505 1106 50-51 -0.04125664156600806 1106 52-53 -0.16905011486846178 1106 54-55 -0.18380426499177815 1106 56-57 -0.07886126535660054 1106 58-59 0.026212689566392555 1106 60-61 -0.015604642100541355 1106 62-63 -0.06565517278109212 1106 64-65 -0.11674021202992435 1106 66-67 0.014479601628899275 1106 68-69 0.06879032932296525 1106 70-71 0.09623601772321777 1106 72-73 0.03899098729407413 1106 74-75 -0.02807217736694767 1106 76-77 -0.10555699762765158 1106 78-79 -0.03225784645091778 1106 80-81 -0.11965473932299986 1106 82-83 -0.13669332237050114 1106 84-85 0.13205824748309425 1106 86-87 0.1050155374374846 1106 88-89 0.20221757026689957 1106 90-91 0.2609871101197889 1106 92-93 0.2826562895491165 1106 94-95 0.46992185774130846 1106 96-97 0.3236057308616864 1106 98-99 0.49596121147171246 1106 100-101 0.8110140483540889 1107 1 -0.007407297252921552 1107 2 -0.09279882223556157 1107 3 0.07269831411752747 1107 4 0.0908282871873638 1107 5 0.005290709187448783 1107 6 -0.08996121803748025 1107 7 0.06291474203677438 1107 8 0.0037259007859091753 1107 9 0.10488226596975636 1107 10-11 0.024864833812742404 1107 12-13 0.18378258520892388 1107 14-15 0.14569808551622287 1107 16-17 0.34486729688969575 1107 18-19 0.275519936747294 1107 20-21 0.1583795234684615 1107 22-23 0.20209566926938294 1107 24-25 0.06658348696709737 1107 26-27 0.13308041146407135 1107 28-29 0.16780076975916103 1107 30-31 0.09444532833898478 1107 32-33 0.1625675881110311 1107 34-35 0.2499468141659733 1107 36-37 0.2534835009845082 1107 38-39 0.19358349968130284 1107 40-41 0.03061022644867961 1107 42-43 0.22734627603794166 1107 44-45 0.4528657555754805 1107 46-47 0.5164329304626207 1107 48-49 0.3283979700073374 1107 50-51 0.4576723218347425 1107 52-53 0.3825527847211134 1107 54-55 0.3513779320411601 1107 56-57 0.4359010213098955 1107 58-59 0.4398048435792319 1107 60-61 0.3865159329229826 1107 62-63 0.36156126892400664 1107 64-65 0.4170628285472091 1107 66-67 0.12878793811932354 1107 68-69 0.28668734315211 1107 70-71 0.3238341604543713 1107 72-73 0.20312454527404 1107 74-75 0.2570612563944614 1107 76-77 0.023815601575016387 1107 78-79 0.18174528203950402 1107 80-81 0.05301019695741971 1107 82-83 -0.05639665788153181 1107 84-85 0.07216099678164056 1107 86-87 0.16680957356288317 1107 88-89 0.3139263003535966 1107 90-91 0.38562138558327774 1107 92-93 0.4354580789572502 1107 94-95 0.43477373562547683 1107 96-97 0.5001149889650378 1107 98-99 0.5399587960763768 1107 100-101 1.0173901261927654 1108 1 0.18767966608306352 1108 2 0.23780991313167377 1108 3 0.1388101373852706 1108 4 0.19931259354414266 1108 5 0.24922013473141647 1108 6 0.23033132206667517 1108 7 0.20224748359189704 1108 8 0.19255356625738074 1108 9 0.2824215454193393 1108 10-11 0.46574062449481346 1108 12-13 0.42341642871712537 1108 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2316 50-51 -2.48172061646466 2316 52-53 -2.6637987034236588 2316 54-55 -2.565753958464036 2316 56-57 -2.22821280359533 2316 58-59 -2.0345095036033456 2316 60-61 -1.8323146274671842 2316 62-63 -2.2397432256888763 2316 64-65 -2.368951211664303 2316 66-67 -2.0638926516688088 2316 68-69 -2.290945586806018 2316 70-71 -2.2317498777192135 2316 72-73 -2.0103508182835768 2316 74-75 -1.969722784678499 2316 76-77 -1.8600296801004177 2316 78-79 -1.774994406997216 2316 80-81 -1.8108333172159128 2316 82-83 -1.9785278751189743 2316 84-85 -1.8876352999947876 2316 86-87 -1.9323465975145915 2316 88-89 -1.9283038691230914 2316 90-91 -2.255937133093841 2316 92-93 -2.1091857066419593 2316 94-95 -2.058316139626232 2316 96-97 -2.287643832306209 2316 98-99 -2.5936982354735356 2316 100-101 -2.4301609215819227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18460.0 3 4656.0 4 4349.0 5 4872.0 6 5702.0 7 6786.0 8 8146.0 9 9497.0 10 10999.0 11 13175.0 12 15706.0 13 18373.0 14 19811.0 15 21210.0 16 24807.0 17 28972.0 18 26958.0 19 21561.0 20 22422.0 21 23920.0 22 25550.0 23 28773.0 24 32868.0 25 36801.0 26 42044.0 27 49089.0 28 56876.0 29 66593.0 30 78786.0 31 94411.0 32 115778.0 33 145932.0 34 190833.0 35 265575.0 36 385109.0 37 544326.0 38 456197.0 39 67263.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55106494313983 18.361751693946015 13.938832351437602 23.148351011476556 2 15.593701223772946 19.856358612860994 40.27905691039498 24.270883252971082 3 19.658123091487422 24.275108342924018 29.264741031455433 26.802027534133128 4 12.967287960385873 16.00290071671262 36.35801430078772 34.67179702211378 5 14.054761691063097 37.06494670383623 33.66282021570311 15.217471389397566 6 32.6509030127877 35.01873179504192 17.428185300654434 14.90217989151594 7 29.248005648054274 32.34361765512641 20.27773395936174 18.130642737457578 8 26.64484679759035 34.68073034486871 19.46542437211496 19.208998485425994 9 26.96436278954606 16.758087883482283 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22.66040119148022 34-35 22.988168899608247 26.2035175934158 28.44984905367459 22.358464453301362 36-37 22.88207611962302 26.05499334080344 28.45170032156347 22.61123021801007 38-39 22.631274643392896 26.22603656939489 28.70592863397504 22.436760153237174 40-41 22.749599970230342 26.06004955575156 28.729496064739617 22.46085440927848 42-43 22.59559986424799 26.230403486626685 28.37448371981966 22.799512929305664 44-45 22.627143201153192 26.366832593359756 28.247745031422326 22.758279174064725 46-47 22.758007941027905 26.518491835257546 27.83213560522667 22.891364618487877 48-49 22.95681239771461 26.561417863144687 27.679791233392592 22.80197850574811 50-51 22.756206894643174 26.723547581873447 27.684807207174572 22.83543831630881 52-53 23.00608588807239 26.701091756595336 27.553411273937417 22.739411081394852 54-55 22.65996179200879 26.4834946073266 27.780121077504933 23.076422523159675 56-57 22.760404518971825 26.967752601769 27.51568053444017 22.756162344819007 58-59 22.91392307114618 26.552651553757933 27.54661542010203 22.98680995499386 60-61 22.88250524806194 26.413851431459484 28.031027034871396 22.67261628560718 62-63 22.77106205269607 26.4924999584966 27.941865727868166 22.794572260939162 64-65 22.58395840979742 26.446974571761956 27.85295208980972 23.116114928630907 66-67 22.579036870536335 26.73537048105811 27.514696663186854 23.170895985218703 68-69 22.439927129596278 26.61667157938497 27.825267388044967 23.118133902973785 70-71 22.499037457377604 26.56869282937202 27.667107045644237 23.26516266760614 72-73 22.6212794853179 26.47233677644637 27.63274348159252 23.273640256643212 74-75 22.5065674802833 26.770795818753147 27.447328900385486 23.27530780057807 76-77 22.672479245601362 26.677999775596394 27.43808732990064 23.211433648901604 78-79 22.666975630064844 26.701815165553356 27.46110054126162 23.170108663120182 80-81 22.51158234211827 26.988265730822814 27.39078859198831 23.1093633350706 82-83 22.601354087897484 26.837679337992526 27.346665586643127 23.214300987466864 84-85 22.676456357694917 26.66660517363257 27.181385723162272 23.47555274551024 86-87 22.711630877219342 26.561808236894567 27.4580649012984 23.268495984587688 88-89 22.769593291404615 26.548897274633127 27.257039832285113 23.42446960167715 90-91 22.679203719447184 26.68789911489342 27.3930118785852 23.2398852870742 92-93 22.83435072374789 26.499153712342714 27.33262543761208 23.33387012629732 94-95 22.66696036025668 26.444719982566276 27.3135541996366 23.574765457540444 96-97 22.817408757391366 26.2854104933886 27.29162790674266 23.605552842477376 98-99 22.77346731645124 26.25437220577257 27.130054773148576 23.842105704627617 100-101 23.02466313638134 25.79291378015471 27.549693065092473 23.632730018371483 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3649.0 1 2425.5 2 1281.5 3 1392.5 4 1270.0 5 1227.5 6 1759.0 7 3112.5 8 4748.0 9 5298.0 10 4438.0 11 3634.0 12 3534.0 13 3629.5 14 3904.5 15 4176.0 16 4373.0 17 4514.0 18 4630.0 19 4982.5 20 5511.0 21 6181.0 22 7171.0 23 8437.5 24 9926.0 25 11989.5 26 15057.0 27 18520.5 28 22021.0 29 25969.5 30 30189.0 31 35333.0 32 40914.0 33 45575.5 34 50558.5 35 55723.0 36 61829.5 37 67832.5 38 71741.5 39 74472.0 40 77185.0 41 82723.5 42 88768.0 43 93464.5 44 97830.5 45 102086.0 46 105103.5 47 109260.0 48 120352.0 49 123099.0 50 116744.0 51 112974.0 52 109272.0 53 109048.5 54 108122.5 55 101296.5 56 92928.5 57 84303.0 58 76189.0 59 68156.0 60 58651.0 61 48145.5 62 39231.5 63 31549.0 64 24363.5 65 19159.0 66 14677.0 67 12022.5 68 9157.0 69 6069.5 70 4528.0 71 3469.0 72 2772.5 73 2277.5 74 1782.5 75 1514.0 76 1140.5 77 723.5 78 504.5 79 372.0 80 259.5 81 193.5 82 151.0 83 108.0 84 90.5 85 78.0 86 53.0 87 38.0 88 29.5 89 19.5 90 14.5 91 12.5 92 9.5 93 7.0 94 7.5 95 8.0 96 6.0 97 2.5 98 2.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27301756573368957 2 0.1696504159074493 3 0.19844889139232946 4 0.16661020561844225 5 0.24248182722651973 6 0.2020236441497334 7 0.24745975396346537 8 0.252437680700411 9 0.14980552676821637 10-11 0.21005848562782348 12-13 0.21638947298789862 14-15 0.17571413203332606 16-17 0.2901897425309383 18-19 0.2377544672716351 20-21 0.2023076198360692 22-23 0.24824486321392325 24-25 0.23721992480323825 26-27 0.34850498494260684 28-29 0.31922208034574207 30-31 0.2948001713208611 32-33 0.19502447870416215 34-35 0.3629042226850469 36-37 0.400856737941223 38-39 0.38189718476527235 40-41 0.3444792119774931 42-43 0.377721071730922 44-45 0.3867414758851188 46-47 0.3920869005690873 48-49 0.41017782223389304 50-51 0.40336240576183324 52-53 0.4027443410327495 54-55 0.39758266532229236 56-57 0.3759336953522201 58-59 0.3511275839281789 60-61 0.31716743273284165 62-63 0.3852213707406153 64-65 0.41801221028633434 66-67 0.42397569969938664 68-69 0.42945476000045435 70-71 0.4285193106807599 72-73 0.4202171979684714 74-75 0.41522256677938835 76-77 0.400188559855727 78-79 0.3741630234256555 80-81 0.3848705772457298 82-83 0.39414154818198766 84-85 0.395778584491453 86-87 0.390950997823744 88-89 0.39970413074374234 90-91 0.4194822020744257 92-93 0.4192483397445021 94-95 0.42708272779694334 96-97 0.4274502257439662 98-99 0.4255960315567146 100-101 0.3996707218394675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2993214.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.301788438346044 #Duplication Level Percentage of deduplicated Percentage of total 1 76.24386727030411 40.63934482963086 2 13.343647086579526 14.224805080096289 3 4.456234279620827 7.125757704121641 4 1.9954040404881297 4.254344160604767 5 1.027724245300189 2.7389770147974755 6 0.6432873408645192 2.0573019448696077 7 0.4277198935672929 1.5958764694457013 8 0.2960958418932386 1.262595033765389 9 0.20588447623066533 0.9876609715307948 >10 1.183967960081857 11.643360686304884 >50 0.10663820772900574 3.901690568345114 >100 0.06418140541284584 6.295312944036444 >500 0.004026693216412559 1.4942268826118998 >1k 0.0011325074669991512 0.890043573513103 >5k 1.258341629999057E-4 0.5010938611692836 >10k+ 6.291708149995284E-5 0.387608275156912 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11558 0.38614011560817235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9081 0.30338625971948546 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5861 0.19580958795462003 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.6704452137401468E-5 0.0 16-17 0.0 0.0 0.0 5.01133564122044E-5 0.0 18-19 0.0 0.0 0.0 6.681780854960587E-5 0.0 20-21 0.0 0.0 0.0 8.352226068700734E-5 0.0 22-23 0.0 0.0 0.0 3.340890427480294E-4 0.0 24-25 0.0 0.0 0.0 6.848825376334603E-4 1.6704452137401468E-5 26-27 0.0 0.0 0.0 0.001102493841068497 3.3408904274802935E-5 28-29 0.0 0.0 0.0 0.003391003783892498 3.3408904274802935E-5 30-31 0.0 0.0 0.0 0.010490395942288122 3.3408904274802935E-5 32-33 0.0 0.0 0.0 0.02557451622236165 3.3408904274802935E-5 34-35 0.0 0.0 0.0 0.04987949408228079 3.3408904274802935E-5 36-37 0.0 0.0 0.0 0.08970290797784589 3.3408904274802935E-5 38-39 0.0 0.0 0.0 0.15069086273149865 3.3408904274802935E-5 40-41 3.3408904274802935E-5 0.0 0.0 0.21375016955018922 3.3408904274802935E-5 42-43 6.681780854960587E-5 0.0 0.0 0.28016707124849743 3.3408904274802935E-5 44-45 6.681780854960587E-5 0.0 0.0 0.36245320247733703 5.01133564122044E-5 46-47 6.681780854960587E-5 0.0 0.0 0.46488490298388285 1.1693116496181028E-4 48-49 6.681780854960587E-5 0.0 0.0 0.5795943758114188 1.670445213740147E-4 50-51 6.681780854960587E-5 0.0 0.0 0.6997495000357475 1.670445213740147E-4 52-53 6.681780854960587E-5 0.0 0.0 0.8183845191155728 1.670445213740147E-4 54-55 6.681780854960587E-5 0.0 0.0 0.9523375208053951 1.670445213740147E-4 56-57 6.681780854960587E-5 0.0 0.0 1.1173608034707843 1.670445213740147E-4 58-59 6.681780854960587E-5 0.0 0.0 1.305052027686627 1.670445213740147E-4 60-61 6.681780854960587E-5 0.0 0.0 1.5092138417099479 1.670445213740147E-4 62-63 6.681780854960587E-5 0.0 0.0 1.711187372503269 1.670445213740147E-4 64-65 6.681780854960587E-5 0.0 0.0 1.916434975915521 1.670445213740147E-4 66-67 6.681780854960587E-5 0.0 0.0 2.158716349716392 1.670445213740147E-4 68-69 6.681780854960587E-5 0.0 0.0 2.4333375428552717 1.670445213740147E-4 70-71 6.681780854960587E-5 0.0 0.0 2.732213600497659 1.670445213740147E-4 72-73 6.681780854960587E-5 0.0 0.0 3.033211123561496 2.0045342564881764E-4 74-75 6.681780854960587E-5 0.0 0.0 3.343312573040217 2.0045342564881764E-4 76-77 6.681780854960587E-5 0.0 0.0 3.6807759151200017 2.0045342564881764E-4 78-79 6.681780854960587E-5 0.0 0.0 4.06327446016222 2.0045342564881764E-4 80-81 6.681780854960587E-5 0.0 0.0 4.483591884843516 2.0045342564881764E-4 82-83 6.681780854960587E-5 0.0 0.0 4.904493965349621 2.0045342564881764E-4 84-85 6.681780854960587E-5 0.0 0.0 5.331593397598701 2.0045342564881764E-4 86-87 6.681780854960587E-5 0.0 0.0 5.7780699943271685 2.0045342564881764E-4 88-89 6.681780854960587E-5 0.0 0.0 6.265505907696543 2.0045342564881764E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15860 0.0 30.147467 1 GGTATCA 10810 0.0 27.743402 1 GTACATG 13795 0.0 27.390593 1 TACATGG 13930 0.0 26.497091 2 ATCAACG 18965 0.0 25.066395 3 TATCAAC 19050 0.0 24.979036 2 TCAACGC 19065 0.0 24.9598 4 ACATGGG 14225 0.0 24.880753 3 CAACGCA 19285 0.0 24.675062 5 AACGCAG 19680 0.0 24.177378 6 TACCTGG 2520 0.0 24.097897 2 GAGTACT 9985 0.0 22.412802 12-13 GTACCTG 2950 0.0 22.394884 1 ACGCAGA 21855 0.0 21.663088 7 CGCAGAG 22040 0.0 21.48125 8 CATGGGG 6725 0.0 21.305496 4 GTACTTT 10810 0.0 20.81259 14-15 AGTACTT 10550 0.0 20.245222 12-13 GCAGAGT 23235 0.0 19.926266 9 GTATACG 145 6.1242166E-4 19.667053 1 >>END_MODULE