FastQCFastQC Report
Thu 26 May 2016
SRR938702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938702_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993214
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114250.38169673133962356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99040.3308817879376483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64370.21505311681690653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112100.030.1688791
GTATCAA157950.028.096821
GTACATG137150.023.5835021
TACATGG139200.023.0504762
ATCAACG194400.022.6581343
TATCAAC195850.022.6115592
TACCTGG26300.022.5593592
TCAACGC195600.022.4705944
ACATGGG138100.022.374833
CAACGCA198650.022.1494875
AACGCAG204300.021.5290166
GAGTACT107100.020.62154812-13
GTACCTG30750.019.4903951
GTACTTT115350.019.22892814-15
ACGCAGA227050.019.182497
AGTACTT111500.019.14823312-13
CGCAGAG229400.019.0066648
CATGGGA75100.018.6446654
CATGGGG66700.018.288574
ATTCGCG6100.017.91336380-81
Produced by FastQC (version 0.11.5)