##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938701_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 968555 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.68500291671614 33.0 31.0 34.0 30.0 34.0 2 31.58349087042037 33.0 31.0 34.0 30.0 34.0 3 31.610510502759265 33.0 31.0 34.0 28.0 34.0 4 35.13846813035914 37.0 35.0 37.0 33.0 37.0 5 35.24589414127231 37.0 35.0 37.0 33.0 37.0 6 35.38223332696646 37.0 35.0 37.0 33.0 37.0 7 35.36446355653525 37.0 35.0 37.0 33.0 37.0 8 35.37177135010402 37.0 35.0 37.0 33.0 37.0 9 36.87782418138361 39.0 37.0 39.0 33.0 39.0 10-11 36.752727516764665 39.0 37.0 39.0 32.5 39.0 12-13 36.628349448405096 39.0 37.0 39.0 32.0 39.0 14-15 37.551538632292434 40.0 37.0 41.0 32.0 41.0 16-17 37.52502697317138 40.0 37.0 41.0 32.0 41.0 18-19 37.678958861396616 40.0 37.0 41.0 32.0 41.0 20-21 37.553568976464945 40.0 37.0 41.0 32.0 41.0 22-23 37.41717300514684 40.0 36.0 41.0 32.0 41.0 24-25 37.40456711286401 40.0 36.0 41.0 32.0 41.0 26-27 37.10859579476643 39.5 36.0 41.0 31.5 41.0 28-29 36.90629855816138 39.0 35.5 41.0 31.0 41.0 30-31 36.6825208687168 39.0 35.0 41.0 31.0 41.0 32-33 36.36692547144974 39.0 35.0 41.0 30.0 41.0 34-35 35.97333501969429 38.0 35.0 41.0 30.0 41.0 36-37 35.528472311846 38.0 35.0 40.0 28.0 41.0 38-39 35.199068715767304 38.0 35.0 40.0 26.5 41.0 40-41 34.957809830107735 38.0 35.0 40.0 25.5 41.0 42-43 34.79585619815086 38.0 35.0 40.0 25.0 41.0 44-45 34.65074053615953 38.0 35.0 40.0 24.0 41.0 46-47 34.22968649173253 38.0 34.0 40.0 21.5 41.0 48-49 33.886184057694194 38.0 33.5 40.0 18.5 41.0 50-51 32.682718585934715 37.0 32.0 39.0 14.0 40.5 52-53 32.678413203173804 37.0 32.5 39.5 13.0 40.0 54-55 33.1935439907904 38.0 33.0 40.0 11.0 41.0 56-57 33.22840158793254 38.0 33.0 40.0 8.0 41.0 58-59 33.098023860286716 38.0 33.0 40.0 7.5 41.0 60-61 32.74227534832818 37.5 32.5 40.0 3.5 41.0 62-63 32.2396848914104 36.5 32.0 39.5 2.0 41.0 64-65 31.693177465399486 36.0 31.0 39.0 2.0 41.0 66-67 31.278693001429964 35.0 31.0 39.0 2.0 40.5 68-69 30.993470685712218 35.0 31.0 38.5 2.0 40.0 70-71 30.490580297453423 35.0 30.0 37.5 2.0 39.5 72-73 29.928338607513254 35.0 29.5 37.0 2.0 39.0 74-75 29.529298284557925 35.0 29.0 36.5 2.0 39.0 76-77 28.98685464428969 34.5 28.5 36.0 2.0 38.5 78-79 28.5553174574495 34.0 28.5 35.5 2.0 37.0 80-81 28.214232026059438 34.0 27.5 35.0 2.0 37.0 82-83 27.80467448931656 34.0 26.5 35.0 2.0 36.5 84-85 27.413700822359083 34.0 26.0 35.0 2.0 36.0 86-87 26.91561604658486 34.0 25.0 35.0 2.0 36.0 88-89 26.38014929456768 33.0 24.5 35.0 2.0 35.0 90-91 25.843740933658903 33.0 21.5 35.0 2.0 35.0 92-93 25.49753653638668 33.0 20.0 35.0 2.0 35.0 94-95 25.122758645611245 33.0 17.0 35.0 2.0 35.0 96-97 24.394999767695175 32.0 2.0 35.0 2.0 35.0 98-99 23.668858247595644 32.0 2.0 35.0 2.0 35.0 100-101 21.584708663937512 29.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31092653169550744 1101 2 0.025471469450113915 1101 3 0.17243014794273748 1101 4 0.1624240607775107 1101 5 -0.040561027356531554 1101 6 0.03615597026477957 1101 7 0.15154854813077634 1101 8 0.3802262198985602 1101 9 0.4832603188577522 1101 10-11 0.4194570523149892 1101 12-13 0.40211185037569663 1101 14-15 0.503412086155528 1101 16-17 0.5651326134779708 1101 18-19 0.3556935453370258 1101 20-21 0.6097653517718484 1101 22-23 0.4786821263595584 1101 24-25 0.6073298575395043 1101 26-27 0.6044819901639187 1101 28-29 0.5013221074326566 1101 30-31 0.7218269880909602 1101 32-33 0.5983788799800536 1101 34-35 0.41753895112996986 1101 36-37 0.6720412462334764 1101 38-39 0.7729787487339408 1101 40-41 0.5360985077047857 1101 42-43 0.4171430710032098 1101 44-45 0.5098826001456729 1101 46-47 0.25641168298335515 1101 48-49 0.026465888680597516 1101 50-51 -0.07111178667612705 1101 52-53 -0.19070911464476836 1101 54-55 -0.061875463173059586 1101 56-57 0.054724975627856054 1101 58-59 -0.07471501646239886 1101 60-61 -0.2662663279806097 1101 62-63 0.023689211094485074 1101 64-65 -0.24320461798879833 1101 66-67 -0.26476941066392 1101 68-69 0.024188643287036626 1101 70-71 -0.05768835421758922 1101 72-73 -0.19401764671748722 1101 74-75 -0.25321338612382505 1101 76-77 -0.027988823948970065 1101 78-79 -0.05547602216740799 1101 80-81 0.026302735038921554 1101 82-83 0.18021528462224978 1101 84-85 0.024823835130302285 1101 86-87 0.0953104099992288 1101 88-89 -0.24261204799835312 1101 90-91 0.05259722118463728 1101 92-93 -0.1713166592590838 1101 94-95 -0.23018157056313981 1101 96-97 -0.2078487971587748 1101 98-99 -0.41552601587311955 1101 100-101 -0.22641451029925364 1102 1 0.3621764858424541 1102 2 0.46190080072923223 1102 3 0.37954379014221473 1102 4 0.34590504093231544 1102 5 0.20743060904712252 1102 6 0.2917592129924813 1102 7 0.3233645123287161 1102 8 0.27327398043559015 1102 9 0.4409792213191466 1102 10-11 0.37107062114958467 1102 12-13 0.30729462063357715 1102 14-15 0.26794979570397004 1102 16-17 0.3519503200918095 1102 18-19 0.4695604247618448 1102 20-21 0.4833072247772421 1102 22-23 0.5622149204610309 1102 24-25 0.5578910988732702 1102 26-27 0.45266712643918794 1102 28-29 0.5569693690884989 1102 30-31 0.39979643161834844 1102 32-33 0.4085380817701605 1102 34-35 0.5875964508548535 1102 36-37 0.6127142773035033 1102 38-39 0.40746115950391015 1102 40-41 0.46223153657386007 1102 42-43 0.4689638956244693 1102 44-45 0.5346363692663942 1102 46-47 0.6028933784457351 1102 48-49 0.6344635776868657 1102 50-51 0.6455668017835734 1102 52-53 0.6670593091107833 1102 54-55 0.6664933604210788 1102 56-57 0.9603043747694855 1102 58-59 1.016312458685249 1102 60-61 0.9659770783507824 1102 62-63 1.1238208093483948 1102 64-65 0.6776965778587432 1102 66-67 0.8572684822967211 1102 68-69 0.7246788123930834 1102 70-71 0.6373177900911315 1102 72-73 0.9392978039262907 1102 74-75 0.888986092985526 1102 76-77 0.8767491717225013 1102 78-79 0.8255307274015742 1102 80-81 0.8817624577196703 1102 82-83 0.8134298585557076 1102 84-85 0.6582218212643411 1102 86-87 0.5655494878026843 1102 88-89 0.6795088389830504 1102 90-91 0.7513793641727915 1102 92-93 0.6169477110868336 1102 94-95 0.5743583399317096 1102 96-97 0.42178006825335146 1102 98-99 0.6097092102741222 1102 100-101 0.6990674052577681 1103 1 0.05525597032572804 1103 2 -0.06483893342375069 1103 3 0.0042674278784708974 1103 4 0.27704702373044654 1103 5 0.019187464179374558 1103 6 0.11571645098757699 1103 7 0.15853464363449632 1103 8 0.18068925180985218 1103 9 0.18622376821984687 1103 10-11 0.15999220167211803 1103 12-13 0.15586326632367076 1103 14-15 0.3321551530875624 1103 16-17 0.2270059295717104 1103 18-19 0.2684192508288774 1103 20-21 0.20113461295162693 1103 22-23 0.29349474736388004 1103 24-25 0.23950154941116608 1103 26-27 0.23937275344449205 1103 28-29 0.4023817555103477 1103 30-31 0.30424249379311874 1103 32-33 0.36188341327007123 1103 34-35 0.5367036659321087 1103 36-37 0.5154768888594887 1103 38-39 0.3648912600728167 1103 40-41 0.4465479781063877 1103 42-43 0.49524021744495883 1103 44-45 0.31540863327997926 1103 46-47 0.2601788943669021 1103 48-49 0.23825108880876655 1103 50-51 0.3355422662069927 1103 52-53 0.21295903679284578 1103 54-55 0.3481695312533972 1103 56-57 0.41009406982731633 1103 58-59 0.2886742101479456 1103 60-61 0.2294531089798042 1103 62-63 0.20553472722162525 1103 64-65 0.023700446930440933 1103 66-67 0.02146136967572332 1103 68-69 0.13026571147367605 1103 70-71 0.1049008335811159 1103 72-73 0.18371157890000234 1103 74-75 0.041049373203378536 1103 76-77 0.17465589608821475 1103 78-79 0.08406762513405042 1103 80-81 0.1255392513781679 1103 82-83 0.13765166286426478 1103 84-85 0.3022554296118294 1103 86-87 0.23864157710139722 1103 88-89 0.13041577904614243 1103 90-91 0.12019491595364329 1103 92-93 0.34813485552318824 1103 94-95 0.14913960931076886 1103 96-97 0.15487984177110903 1103 98-99 0.4266883011096816 1103 100-101 0.43835162699467034 1104 1 0.3183890641138589 1104 2 0.34218097806823167 1104 3 0.31322563117699787 1104 4 0.3838718127226173 1104 5 0.1897492707775541 1104 6 0.46362492897584673 1104 7 0.28028096189222396 1104 8 0.19805459198090603 1104 9 0.25953697075924964 1104 10-11 0.25625675854160335 1104 12-13 0.29121698911476557 1104 14-15 0.38865858038334267 1104 16-17 0.40159895576985605 1104 18-19 0.47621177994261643 1104 20-21 0.4764513355684059 1104 22-23 0.4221473899523218 1104 24-25 0.4156656772360847 1104 26-27 0.40894826360267444 1104 28-29 0.41262090709297894 1104 30-31 0.5491634045147293 1104 32-33 0.5404716710566859 1104 34-35 0.4593896086774265 1104 36-37 0.6391294471010269 1104 38-39 0.5210072539323605 1104 40-41 0.4359866213583601 1104 42-43 0.6847935724835565 1104 44-45 0.6054731305443326 1104 46-47 0.3974332340150042 1104 48-49 0.34685559743182637 1104 50-51 0.4021324583492287 1104 52-53 0.4006314559182229 1104 54-55 0.3717501134719541 1104 56-57 0.22206908065184194 1104 58-59 0.504190103915839 1104 60-61 0.5085035877890505 1104 62-63 0.5215955019760301 1104 64-65 0.3455361729042217 1104 66-67 0.2307339057621398 1104 68-69 0.26134810715061363 1104 70-71 0.23965850493184604 1104 72-73 0.32960529055730703 1104 74-75 0.24887114257645493 1104 76-77 0.15048825943217636 1104 78-79 0.17577182617208464 1104 80-81 0.169653243404575 1104 82-83 0.09707799146854512 1104 84-85 -0.19079607260649212 1104 86-87 -0.2413389668798871 1104 88-89 -0.2770196815160624 1104 90-91 -0.0896005709247909 1104 92-93 0.020914985642342288 1104 94-95 -0.007147327603370002 1104 96-97 0.005653302861883702 1104 98-99 0.221869843758288 1104 100-101 0.51977618993714 1105 1 0.11795742484257232 1105 2 0.04271686442200817 1105 3 0.12885593157764674 1105 4 0.07142020309131425 1105 5 0.3011257267148437 1105 6 0.19583953145636457 1105 7 0.07961378056428714 1105 8 0.04386583150689205 1105 9 0.22646979619328533 1105 10-11 0.1819900099799341 1105 12-13 0.23134479040292177 1105 14-15 0.32409808044814525 1105 16-17 0.25502812128604546 1105 18-19 0.3459068713137796 1105 20-21 0.2897701682264895 1105 22-23 0.33515573535389365 1105 24-25 0.18829152036958163 1105 26-27 0.2971379144031232 1105 28-29 0.20560536019024056 1105 30-31 0.28066518235011273 1105 32-33 0.35885127694636765 1105 34-35 0.5795397244762128 1105 36-37 0.4972491678315478 1105 38-39 0.38517161495409624 1105 40-41 0.3010165149136341 1105 42-43 0.5038324707327675 1105 44-45 0.7137503488940666 1105 46-47 0.7323644591154519 1105 48-49 0.6922876029766485 1105 50-51 1.0564671647387343 1105 52-53 0.9915382928363385 1105 54-55 1.1107548637579256 1105 56-57 0.9421273463184789 1105 58-59 0.6357510490142531 1105 60-61 0.9112776871787815 1105 62-63 0.9731871013516766 1105 64-65 1.278760563340544 1105 66-67 1.08912072442293 1105 68-69 0.9072759907094401 1105 70-71 0.668454366604422 1105 72-73 0.9275090789526352 1105 74-75 0.9844520805747976 1105 76-77 1.2563080641238606 1105 78-79 1.111431090356728 1105 80-81 1.2750053449694079 1105 82-83 1.2399387568111848 1105 84-85 0.9225131805899487 1105 86-87 1.1943473451964302 1105 88-89 1.054750321245077 1105 90-91 1.1213594601802832 1105 92-93 1.584047582296293 1105 94-95 1.5282059573067244 1105 96-97 1.1130559727141893 1105 98-99 1.0640690627504128 1105 100-101 1.3669122126573683 1106 1 0.17537347403944636 1106 2 0.25919424802268054 1106 3 0.2483980839965092 1106 4 0.18559775562503233 1106 5 0.29080464242115767 1106 6 0.33276450399777247 1106 7 0.3218280857922906 1106 8 0.4616032437648556 1106 9 0.3233459286858533 1106 10-11 0.3061591437381068 1106 12-13 0.2774462282851786 1106 14-15 0.440156755410932 1106 16-17 0.34423257487407 1106 18-19 0.32514650534793077 1106 20-21 0.3713414128114252 1106 22-23 0.37509139583750084 1106 24-25 0.41220530318747706 1106 26-27 0.31445996323201797 1106 28-29 0.47833689045602057 1106 30-31 0.3353275671494842 1106 32-33 0.4564522046842825 1106 34-35 0.4705176962761044 1106 36-37 0.6314617335509567 1106 38-39 0.5408046678474179 1106 40-41 0.38270874455106707 1106 42-43 0.4475811117416839 1106 44-45 0.5858624579924836 1106 46-47 0.48696440001675967 1106 48-49 0.5040097722330614 1106 50-51 0.7238736800904491 1106 52-53 0.7310702981217716 1106 54-55 0.7260324324601513 1106 56-57 0.8092461737112799 1106 58-59 0.48669300406579197 1106 60-61 0.6275002773739757 1106 62-63 0.6129488109763983 1106 64-65 0.41804558070775144 1106 66-67 0.5607483357765695 1106 68-69 0.6886236638379373 1106 70-71 0.6016594669328086 1106 72-73 0.4605279626564496 1106 74-75 0.5223906513900829 1106 76-77 0.30318893566226635 1106 78-79 0.651161578032422 1106 80-81 0.5202799587371736 1106 82-83 0.6108911935170447 1106 84-85 0.6444499449924663 1106 86-87 0.8290059387591349 1106 88-89 0.6945220664962761 1106 90-91 0.8184123312057565 1106 92-93 0.7800327741718966 1106 94-95 0.9477659508393188 1106 96-97 1.0733215822948665 1106 98-99 1.310416373481285 1106 100-101 1.6792108729012334 1107 1 0.058833758738121134 1107 2 0.032545927875908376 1107 3 0.012409419524871623 1107 4 0.19991459347127716 1107 5 0.015023686764038757 1107 6 0.12879051674205044 1107 7 -0.07359838889114911 1107 8 0.002782990517353312 1107 9 -0.1716104164252883 1107 10-11 -0.002426044723243592 1107 12-13 -0.1626998886411215 1107 14-15 -0.17990319839515934 1107 16-17 0.1229424213857726 1107 18-19 0.18139988389233253 1107 20-21 0.10202596733498837 1107 22-23 0.13835904207728333 1107 24-25 -0.044415005754814274 1107 26-27 -0.08247682317844607 1107 28-29 -0.04171814910366578 1107 30-31 0.07707409032015278 1107 32-33 -0.046375125078036206 1107 34-35 -0.07925379126628229 1107 36-37 -0.06431365262690036 1107 38-39 -0.03832288960830965 1107 40-41 -0.27741050782824317 1107 42-43 -0.01960646504340957 1107 44-45 0.12424599854320206 1107 46-47 0.17304070188253462 1107 48-49 0.22852367135996587 1107 50-51 0.14006284581522266 1107 52-53 -0.05932726441201197 1107 54-55 0.061026781076790826 1107 56-57 -0.0074458832284491905 1107 58-59 0.2568029193769519 1107 60-61 0.1716990094984041 1107 62-63 0.05329641200511759 1107 64-65 0.045404828500434036 1107 66-67 -0.05890864982468713 1107 68-69 -0.008480206950142843 1107 70-71 0.12264002289788678 1107 72-73 -0.06270168007783639 1107 74-75 -0.09992330343161271 1107 76-77 -0.12038142815577757 1107 78-79 -0.047729440363237785 1107 80-81 -0.04173619275266205 1107 82-83 0.12770938278105248 1107 84-85 -0.07351490176494835 1107 86-87 -0.14699615798599552 1107 88-89 -0.06178373067639242 1107 90-91 0.12179824697557606 1107 92-93 0.006101213243429271 1107 94-95 0.08349907449814253 1107 96-97 0.2134120044286334 1107 98-99 0.15798955480059718 1107 100-101 0.3982732346880553 1108 1 0.24040767200047242 1108 2 0.06660165543008745 1108 3 0.008973642133042858 1108 4 0.08985959586247816 1108 5 0.04537620004149545 1108 6 0.11560745147486529 1108 7 0.06685002410691254 1108 8 0.11661356767761788 1108 9 0.12403634035187139 1108 10-11 0.20373947668429793 1108 12-13 0.26569309884911974 1108 14-15 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1016.0 6 1170.0 7 1465.0 8 1913.0 9 2322.0 10 3219.0 11 4781.0 12 7204.0 13 9682.0 14 9105.0 15 9289.0 16 11413.0 17 15137.0 18 12491.0 19 8230.0 20 7912.0 21 8271.0 22 8859.0 23 9859.0 24 10803.0 25 12405.0 26 14038.0 27 16143.0 28 18765.0 29 21948.0 30 26044.0 31 31286.0 32 37845.0 33 47915.0 34 66922.0 35 111475.0 36 118428.0 37 152174.0 38 121895.0 39 21893.0 40 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.76819062214653 16.354936749177632 12.00054186922131 16.876330759454532 2 12.451028922259093 24.287691066001283 41.813420581338754 21.44785943040087 3 23.468498863832465 33.749600868840076 28.71057643322962 14.071323834097841 4 10.135979404137982 16.241771938654757 40.38651347236849 33.23573518483877 5 10.696424325749195 43.396735478668965 31.818022059811657 14.08881813577018 6 21.694286791400494 35.86312674616167 23.876698392369306 18.565888070068528 7 25.087201945969394 38.26742618692255 21.388000888287518 15.25737097882054 8 22.290407981762346 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16.523891581848762 32-33 17.294201986833638 30.761058308619287 32.12557317475025 19.81916652979682 34-35 19.222787840630915 27.116322946480715 32.59955390242185 21.061335310466518 36-37 21.938462208705285 26.960151775100666 34.49247866829589 16.608907347898157 38-39 23.641164288166195 24.40008586868935 35.00938022095632 16.949369622188136 40-41 18.492277560939954 27.435511555535047 37.76449736105286 16.307713522472135 42-43 17.239380147096572 31.295125008232244 33.4955582186826 17.969936625988584 44-45 17.3864294056512 32.08085350902486 30.6673277226552 19.865389362668733 46-47 17.77501995331521 29.222336718611842 28.859676463282007 24.142966864790942 48-49 19.93927272500939 29.23462263818204 29.80989271583909 21.01621192096948 50-51 22.540126587793054 30.129274308818616 30.725857143746225 16.6047419596421 52-53 18.212108418299866 30.382040794309585 33.263295805943315 18.14255498144723 54-55 15.97823431818618 29.902347973962918 34.47771543321061 19.641702274640295 56-57 19.122635453599877 32.39966524836228 29.61245342461918 18.86524587341867 58-59 18.229949398835775 29.809637808468292 30.794133976704074 21.166278815991856 60-61 18.533687956126833 27.507797106286297 32.86181008954364 21.096704848043235 62-63 23.841890641787764 26.48753842388441 30.946264638411026 18.7243062959168 64-65 21.62203660802563 28.065426756455892 33.23667608339982 17.07586055211866 66-67 17.962319626962337 28.87908697278317 34.36405616729633 18.79453723295816 68-69 16.895360461852693 32.967392133293146 32.004766694975615 18.132480709878546 70-71 17.59285198466059 32.23937322575826 28.858405133057758 21.3093696565234 72-73 17.920422036575463 30.130952720595296 28.04748430201344 23.9011409408158 74-75 18.74174631183977 30.68660311650966 30.06334268016511 20.50830789148546 76-77 21.43869528035883 27.41802069325677 31.619993351522435 19.52329067486196 78-79 18.546666085971868 27.860284830800055 34.28359889750944 19.309450185718642 80-81 17.646665370406378 32.64745608918271 31.586959621491996 18.11891891891892 82-83 17.585279379712787 31.87326053711969 29.283855969491757 21.25760411367576 84-85 18.782730624015066 28.7557086564155 29.773530917103074 22.68802980246636 86-87 20.98894892670943 28.487176574981497 31.42826915267844 19.095605345630627 88-89 22.505932113284562 25.86017587581331 32.0719996473148 19.561892363587326 90-91 18.700748092165288 27.99513063215163 34.49190885132608 18.812212424357007 92-93 17.546118235927228 31.678721941984257 31.73889025766044 19.036269564428075 94-95 17.14526694068607 32.71997111799502 29.700380321229314 20.434381620089592 96-97 17.50271420427519 29.833890179638047 28.01494537096546 24.648450245121307 98-99 19.64740585672057 29.566651227035713 28.403958838946668 22.38198407729705 100-101 22.131976062438056 29.40856754778981 29.665700817816564 18.79375557195557 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8865.0 1 5619.5 2 8462.0 3 10838.5 4 5093.0 5 3442.5 6 5070.0 7 8456.5 8 10525.0 9 9433.0 10 6741.5 11 4666.0 12 4289.0 13 4222.5 14 4333.5 15 4373.0 16 4201.0 17 4078.0 18 3984.5 19 4066.5 20 4212.0 21 4373.0 22 4699.5 23 5190.5 24 5777.0 25 6462.5 26 7553.0 27 8757.0 28 9530.5 29 10457.0 30 11660.5 31 12879.5 32 14240.0 33 15672.5 34 17402.5 35 18604.5 36 19576.0 37 20760.0 38 20698.5 39 19785.0 40 18868.5 41 18712.0 42 18613.5 43 18765.0 44 22181.0 45 25208.5 46 30496.5 47 47868.5 48 114522.0 49 112410.0 50 48945.0 51 32820.5 52 19134.5 53 13280.0 54 11060.0 55 9500.0 56 8226.5 57 7154.5 58 6375.5 59 5807.5 60 5304.0 61 4607.0 62 3789.0 63 2642.0 64 1568.0 65 1151.0 66 872.0 67 681.5 68 582.5 69 521.5 70 459.0 71 348.5 72 282.5 73 285.0 74 252.5 75 201.0 76 138.0 77 81.0 78 59.0 79 49.0 80 33.5 81 24.0 82 13.5 83 9.0 84 8.5 85 4.5 86 4.0 87 2.5 88 1.5 89 2.5 90 4.0 91 2.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15817377433393043 2 0.09209595737980807 3 0.08548817568439583 4 0.10138815038898152 5 0.12234720795411723 6 0.03716877203669384 7 0.04129863559632648 8 0.046254471867885665 9 0.04563499233394077 10-11 0.04372493043761067 12-13 0.04780317070274791 14-15 0.04604797868990403 16-17 0.04480901962201424 18-19 0.049506739421096374 20-21 0.05095219166696781 22-23 0.06483885788623259 24-25 0.08135831212476319 26-27 0.08331999731558869 28-29 0.07242748217705758 30-31 0.06184470680549891 32-33 0.04976485589357341 34-35 0.2939430388568538 36-37 0.4642999106917005 38-39 0.44313435994858325 40-41 0.4192327745972092 42-43 0.45000025811647254 44-45 0.4585181017082148 46-47 0.4573307659348204 48-49 0.47214665145500256 50-51 0.46264796526784746 52-53 0.4695654867302322 54-55 0.4613573829054623 56-57 0.4396239759228955 58-59 0.41830355529629193 60-61 0.38965262685134044 62-63 0.44509604513940876 64-65 0.48195507740913013 66-67 0.4836586461274785 68-69 0.4939833050265602 70-71 0.4917635033632576 72-73 0.4808709882247265 74-75 0.4741083366458281 76-77 0.4568145329898663 78-79 0.43291294763849236 80-81 0.43802365379353775 82-83 0.4497937649384908 84-85 0.4480385729256469 86-87 0.4584664784137194 88-89 0.4669326987109664 90-91 0.4717852883935347 92-93 0.47415995994032345 94-95 0.4783414467944515 96-97 0.4795287825678459 98-99 0.4734372338173877 100-101 0.4608411499605082 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 968555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.323248156758076 #Duplication Level Percentage of deduplicated Percentage of total 1 84.8965053321993 37.62888873480604 2 9.4645027509253 8.389950082191632 3 2.407971441830898 3.201873473119723 4 0.960248005958967 1.702452426406056 5 0.5061216618868175 1.1216478008660111 6 0.31397693723118536 0.834988662263801 7 0.2106799671404929 0.6536614325657993 8 0.17495370266821195 0.6203613103445461 9 0.12100031319847906 0.48268142180474727 >10 0.7938551383934166 6.67627628708448 >50 0.07695814610795625 2.333353124190252 >100 0.050838851389180525 4.590307818622232 >500 0.008395406651196473 2.5614721802267244 >1k 0.010727464054306603 10.702655823300745 >5k 0.0018656459224881048 6.375306934022998 >10k+ 0.0013992344418660787 12.12412248818424 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 32431 3.3483901275611605 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 28574 2.9501680338235827 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 19189 1.9811987961447723 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15082 1.5571650551594902 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11655 1.2033389946879631 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10364 1.0700476483008192 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9186 0.9484231664696376 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8914 0.9203400942641358 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8804 0.908982969475146 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8051 0.8312382879650613 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7133 0.7364579192714921 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7010 0.7237585888256216 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6355 0.656132073036637 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6225 0.6427100164678309 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4951 0.5111738620935311 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4790 0.4945511612660097 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4689 0.48412325577793724 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4593 0.4742115832348188 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4094 0.4226915353284016 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4078 0.42103958990454854 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3891 0.40173247776326587 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3647 0.3765403100495067 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3643 0.37612732369354346 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3239 0.3344157017412537 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2984 0.3080878215485956 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2917 0.30117030008621093 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2811 0.2902261616531844 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2722 0.28103721523300174 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2313 0.23880936033575792 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2233 0.2305496332164926 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2203 0.2274522355467681 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2152 0.22218665950823646 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2082 0.21495939827887936 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1922 0.19843994404034876 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1830 0.18894125785319368 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1796 0.18543087382750592 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1780 0.18377892840365287 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1698 0.17531270810640592 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1690 0.1744867353944794 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1676 0.17304128314860798 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1671 0.17252505020365388 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1653 0.1706666116018192 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1570 0.16209714471558145 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1565 0.16158091177062736 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1552 0.16023870611374677 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1470 0.15177248581649982 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1458 0.15053352674861004 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1393 0.14382249846420697 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1391 0.14361600528622537 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1378 0.14227379962934472 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1370 0.14144782691741822 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1346 0.13896990878163862 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1332 0.1375244565357672 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1323 0.13659523723484984 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1223 0.12627057833576824 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1125 0.11615241261466824 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1114 0.11501670013576927 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1112 0.11481020695778762 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1046 0.10799593208439377 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1027 0.10603424689356825 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.548698834862243E-4 0.0 14-15 0.0 5.1623294495408105E-5 0.0 2.0649317798163242E-4 0.0 16-17 0.0 1.0324658899081621E-4 0.0 4.1298635596326484E-4 0.0 18-19 0.0 1.0324658899081621E-4 0.0 6.711028284403054E-4 0.0 20-21 0.0 1.0324658899081621E-4 0.0 0.0012389590678897945 0.0 22-23 0.0 1.0324658899081621E-4 0.0 0.003458760731192343 0.0 24-25 0.0 1.0324658899081621E-4 0.0 0.007743494174311217 0.0 26-27 0.0 1.0324658899081621E-4 0.0 0.011511994672476008 0.0 28-29 0.0 1.0324658899081621E-4 0.0 0.017913283189906613 0.0 30-31 0.0 1.0324658899081621E-4 0.0 0.03164507952568517 0.0 32-33 0.0 1.0324658899081621E-4 0.0 0.05931516537522391 0.0 34-35 0.0 1.0324658899081621E-4 0.0 0.11826896768897997 0.0 36-37 0.0 1.0324658899081621E-4 0.0 0.20804187681649466 0.0 38-39 0.0 1.0324658899081621E-4 0.0 0.3050420471733665 0.0 40-41 0.0 1.0324658899081621E-4 0.0 0.4132444724357419 0.0 42-43 0.0 1.0324658899081621E-4 0.0 0.5143745063522464 0.0 44-45 0.0 1.0324658899081621E-4 0.0 0.6297009462549881 0.0 46-47 0.0 1.0324658899081621E-4 0.0 0.7773951918063506 0.0 48-49 0.0 1.0324658899081621E-4 0.0 0.9575088663008295 0.0 50-51 0.0 1.0324658899081621E-4 0.0 1.1238907444595299 0.0 52-53 0.0 3.0973976697244867E-4 0.0 1.2875365880099736 0.0 54-55 0.0 3.0973976697244867E-4 0.0 1.468837598277847 0.0 56-57 0.0 3.0973976697244867E-4 0.0 1.6758470092044333 0.0 58-59 0.0 4.1298635596326484E-4 0.0 1.9687059588768836 0.0 60-61 0.0 4.1298635596326484E-4 0.0 2.3703351900511587 0.0 62-63 0.0 4.1298635596326484E-4 0.0 2.709551858180485 0.0 64-65 0.0 4.1298635596326484E-4 0.0 2.9970936085199087 0.0 66-67 0.0 4.1298635596326484E-4 0.0 3.3071431152593296 0.0 68-69 0.0 4.1298635596326484E-4 0.0 3.642126673240033 0.0 70-71 0.0 4.1298635596326484E-4 0.0 4.039161431204216 0.0 72-73 0.0 4.1298635596326484E-4 0.0 4.46711854257115 0.0 74-75 0.0 4.1298635596326484E-4 0.0 4.872258157771112 0.0 76-77 0.0 4.1298635596326484E-4 0.0 5.253444564325206 0.0 78-79 0.0 4.1298635596326484E-4 0.0 5.681917908637093 0.0 80-81 0.0 4.1298635596326484E-4 0.0 6.168260966078334 0.0 82-83 0.0 4.1298635596326484E-4 0.0 6.672568930003974 0.0 84-85 0.0 4.1298635596326484E-4 0.0 7.406084321489228 0.0 86-87 0.0 4.1298635596326484E-4 0.0 8.211510962206585 0.0 88-89 0.0 4.1298635596326484E-4 0.0 8.794647696826717 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGTC 20 0.002094175 71.07612 5 TACCTGG 1665 0.0 59.7637 2 ACCTGGG 1710 0.0 57.636776 3 TCACGCA 80 7.2759576E-11 53.30709 4 TATCACG 85 1.3278623E-10 50.171375 2 ATCACGC 90 2.3101165E-10 47.38408 3 CCTGGGA 1400 0.0 42.645668 4 TATAACG 115 5.638867E-11 41.203545 2 AGTACTC 765 0.0 39.64158 5 GTACCCG 60 8.394633E-5 39.498978 1 GTACCTG 2675 0.0 37.74183 1 CCTGGGG 725 0.0 35.292965 4 AAGTACT 860 0.0 35.26257 4 TACCCGG 55 0.002610517 34.461147 2 AAAGTAC 925 0.0 32.78466 3 ATAACGC 135 1.2256351E-8 31.589386 3 GTCGGGA 270 0.0 29.834421 2 GCTTATG 225 0.0 29.49257 1 CTGGGAG 580 0.0 29.410805 5 CGGGAGT 285 0.0 28.264185 4 >>END_MODULE