##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938701_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 968555 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.935644336150244 31.0 31.0 34.0 30.0 34.0 2 32.0616299539004 33.0 31.0 34.0 30.0 34.0 3 32.15940344120881 34.0 31.0 34.0 30.0 34.0 4 35.74980150843267 37.0 35.0 37.0 35.0 37.0 5 35.70705019332924 37.0 35.0 37.0 33.0 37.0 6 35.80809556504277 37.0 35.0 37.0 33.0 37.0 7 35.779603636344866 37.0 35.0 37.0 33.0 37.0 8 35.80121417988653 37.0 35.0 37.0 33.0 37.0 9 37.3904961514834 39.0 37.0 39.0 34.0 39.0 10-11 37.28981678892784 39.0 37.0 39.0 33.5 39.0 12-13 37.221554790383614 39.0 37.0 39.0 33.0 39.0 14-15 38.17466690069227 40.0 37.0 41.0 33.0 41.0 16-17 38.13514823629015 40.0 37.5 41.0 33.0 41.0 18-19 38.352387319253936 40.0 37.5 41.0 33.5 41.0 20-21 38.42819302982278 40.0 38.0 41.0 34.0 41.0 22-23 38.33488857111883 40.0 37.5 41.0 34.0 41.0 24-25 38.20901394345184 40.0 37.0 41.0 33.0 41.0 26-27 37.80989515308888 40.0 36.5 41.0 32.5 41.0 28-29 37.54585129393788 39.5 36.0 41.0 33.0 41.0 30-31 37.30825198362509 39.0 35.5 41.0 32.0 41.0 32-33 36.990801761386805 39.0 35.0 41.0 32.0 41.0 34-35 36.82425468868573 39.0 35.0 41.0 31.0 41.0 36-37 36.522782908559655 39.0 35.0 41.0 31.0 41.0 38-39 36.271066692134156 39.0 35.0 41.0 30.0 41.0 40-41 36.02004790641729 38.5 35.0 41.0 30.0 41.0 42-43 35.92284795391072 39.0 35.0 41.0 30.0 41.0 44-45 35.66136048030313 39.0 35.0 41.0 29.0 41.0 46-47 35.184363304097346 38.5 35.0 41.0 25.5 41.0 48-49 34.80840530480974 38.0 35.0 40.5 24.0 41.0 50-51 34.55404649193902 38.0 34.5 40.0 23.0 41.0 52-53 34.143159655362886 38.0 34.0 40.0 22.5 41.0 54-55 33.96854386173217 38.0 34.0 40.0 22.0 41.0 56-57 33.875895535101264 38.0 34.0 40.0 21.0 41.0 58-59 33.70975267279607 38.0 33.5 40.0 20.0 41.0 60-61 33.29929224463247 37.5 33.0 40.0 18.5 41.0 62-63 32.77084884183139 36.5 32.5 39.5 15.0 41.0 64-65 32.24127385641496 36.0 32.0 39.0 12.5 40.5 66-67 31.861942274832096 35.0 31.0 39.0 8.0 40.0 68-69 31.569191734078085 35.0 31.0 38.0 7.5 40.0 70-71 31.052874126921033 35.0 31.0 37.5 3.5 39.0 72-73 30.604703398361476 35.0 30.5 37.0 2.0 39.0 74-75 30.200574567267733 35.0 30.0 36.0 2.0 39.0 76-77 28.052823536092426 32.0 26.5 34.5 2.0 36.5 78-79 29.035334596383272 34.0 29.0 35.0 2.0 37.0 80-81 29.206116327931817 34.0 29.0 35.0 2.0 37.0 82-83 28.969158695169607 34.0 29.0 35.0 2.0 36.0 84-85 28.64460717254054 34.0 29.0 35.0 2.0 36.0 86-87 27.99206188600544 34.0 27.5 35.0 2.0 35.5 88-89 27.304801482621016 33.0 25.5 35.0 2.0 35.0 90-91 26.76237539427291 33.0 25.0 35.0 2.0 35.0 92-93 26.611196060110164 33.0 25.0 35.0 2.0 35.0 94-95 26.362131732322894 33.0 25.0 35.0 2.0 35.0 96-97 25.68691710847603 33.0 23.0 35.0 2.0 35.0 98-99 24.909932838093862 32.5 10.5 35.0 2.0 35.0 100-101 21.910338597188595 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05943916122381765 1101 2 0.05784089123022795 1101 3 -0.04092158638264465 1101 4 0.14283390029172693 1101 5 0.18291070274147359 1101 6 0.23074793854114262 1101 7 0.1703151053793306 1101 8 0.2538312113191665 1101 9 0.35012002660670305 1101 10-11 0.2537873105502726 1101 12-13 0.2363962252705889 1101 14-15 0.31093947240806585 1101 16-17 0.22757762334187248 1101 18-19 0.324075530985688 1101 20-21 0.21682677167412834 1101 22-23 0.2786984542116926 1101 24-25 0.2800564816389226 1101 26-27 0.20996467227032412 1101 28-29 0.2481322043746914 1101 30-31 0.2666890976881575 1101 32-33 0.2040034617624258 1101 34-35 -0.1756561318901504 1101 36-37 -0.23356268804240443 1101 38-39 0.05438613263351755 1101 40-41 -0.10846563563365663 1101 42-43 -0.03428929990091234 1101 44-45 0.017779809454616213 1101 46-47 0.04179302631626314 1101 48-49 -0.23768339359637025 1101 50-51 -0.29391598466222746 1101 52-53 -0.18046472809822234 1101 54-55 -0.02189439308502017 1101 56-57 -0.14262709321894107 1101 58-59 -0.21340112479699513 1101 60-61 -0.23027265876181957 1101 62-63 -0.32786333871822393 1101 64-65 -0.40560309423318586 1101 66-67 -0.525509673282265 1101 68-69 -0.5058039045898859 1101 70-71 -0.6551503492795021 1101 72-73 -0.7524059001975836 1101 74-75 -0.4779014018139591 1101 76-77 -0.5362684363541845 1101 78-79 -0.3190104906135538 1101 80-81 -0.3189019317563506 1101 82-83 -0.21850637203697687 1101 84-85 -0.24177812289977396 1101 86-87 0.04764794023424557 1101 88-89 0.20591241666861393 1101 90-91 0.14380508206416565 1101 92-93 0.018507076552754853 1101 94-95 -0.10137947472379238 1101 96-97 0.06453107991585938 1101 98-99 0.2799763270814566 1101 100-101 0.9407666730940569 1102 1 0.05430391496560816 1102 2 0.09146951820649463 1102 3 0.1433388956246162 1102 4 0.2020118468087304 1102 5 0.08926501843055235 1102 6 0.14827664255053463 1102 7 0.10313121630702682 1102 8 0.06868118013909452 1102 9 0.17912024670133775 1102 10-11 0.06335500799351479 1102 12-13 0.18790891739487137 1102 14-15 0.06839976366664047 1102 16-17 0.23399017240463849 1102 18-19 0.3569109001944497 1102 20-21 0.16432273660571894 1102 22-23 0.23484459705311167 1102 24-25 0.14007399750408212 1102 26-27 0.28568181397459114 1102 28-29 0.25900396256404434 1102 30-31 0.3900865335855954 1102 32-33 0.600471859840269 1102 34-35 0.5607690726144554 1102 36-37 0.322270278466533 1102 38-39 0.3296190661282523 1102 40-41 0.5002977993100401 1102 42-43 0.35558772221860124 1102 44-45 0.29043809418372035 1102 46-47 0.42584900371988255 1102 48-49 0.2661476287432407 1102 50-51 0.18290988379454376 1102 52-53 0.28097285672992456 1102 54-55 0.5698965353475316 1102 56-57 0.36305409969401126 1102 58-59 0.5432957131766685 1102 60-61 0.4459589541650715 1102 62-63 0.2783804711200091 1102 64-65 0.09646487832834794 1102 66-67 0.21157352249191774 1102 68-69 0.2538435771606018 1102 70-71 0.39417377676135956 1102 72-73 0.5433027128393562 1102 74-75 0.7184665780840618 1102 76-77 0.8538149244909796 1102 78-79 0.650898445228858 1102 80-81 0.7149513751656258 1102 82-83 0.557432698429988 1102 84-85 0.5227733425637062 1102 86-87 0.45258081136883277 1102 88-89 0.7668969733613231 1102 90-91 1.0539972980503727 1102 92-93 1.2665376880490307 1102 94-95 1.0915724529384363 1102 96-97 1.037961530009401 1102 98-99 1.382925595266773 1102 100-101 1.4340308600277716 1103 1 -0.13598835497965922 1103 2 -0.10881223649166571 1103 3 -0.1371654318040001 1103 4 -0.09811757161721602 1103 5 -0.1444801620811873 1103 6 -0.04571618551169365 1103 7 -0.020483602102743248 1103 8 0.12089005608613945 1103 9 -0.03633110005915796 1103 10-11 -0.15016854346379915 1103 12-13 -0.03274107316837416 1103 14-15 0.0316739150322789 1103 16-17 -0.23521397168121183 1103 18-19 -0.05199066091532245 1103 20-21 -0.02508483706841247 1103 22-23 -0.06788330070632753 1103 24-25 -0.017117150814833337 1103 26-27 -0.18131985768008008 1103 28-29 -0.055010974479280605 1103 30-31 -0.041276201996382156 1103 32-33 -0.043421882239599086 1103 34-35 -0.21440854069407322 1103 36-37 -0.4542689245316609 1103 38-39 -0.17438818669603506 1103 40-41 -0.533347621165035 1103 42-43 -0.09999041415606769 1103 44-45 -0.10342026004529714 1103 46-47 -0.07372971234148906 1103 48-49 -0.07729146371004703 1103 50-51 -0.004386214779152908 1103 52-53 0.3111772299942359 1103 54-55 -0.11484658255803737 1103 56-57 -0.034968224984460505 1103 58-59 -0.227177001105197 1103 60-61 -0.22301634848530938 1103 62-63 -0.07945642122003704 1103 64-65 -0.03486786183719914 1103 66-67 -0.2490145224809872 1103 68-69 -0.15746772807814935 1103 70-71 -0.23948782101695443 1103 72-73 -0.09273661148861123 1103 74-75 -0.061705777600909784 1103 76-77 -0.21588397545239957 1103 78-79 -0.21179163808425372 1103 80-81 0.13576347578845116 1103 82-83 0.10189332017767683 1103 84-85 0.04378530028702343 1103 86-87 0.07152443346702952 1103 88-89 0.24535756814684007 1103 90-91 0.4426637490196619 1103 92-93 0.27770171242283226 1103 94-95 0.303314815903196 1103 96-97 0.4020671273563252 1103 98-99 0.6028863653064072 1103 100-101 0.7161899907673437 1104 1 -0.01628660562491291 1104 2 0.0745331865642811 1104 3 0.09875847604419619 1104 4 0.07576750291732992 1104 5 0.07623768628557315 1104 6 0.05091387459953012 1104 7 0.005702174760337186 1104 8 0.10920519860428612 1104 9 0.16692019185422424 1104 10-11 0.12097075825631975 1104 12-13 0.1589490700526639 1104 14-15 0.22750324188776716 1104 16-17 0.21193763344033556 1104 18-19 0.25233802209216094 1104 20-21 0.17834245871367926 1104 22-23 0.13922208231295485 1104 24-25 0.2019626807162922 1104 26-27 0.31627850486540154 1104 28-29 0.31070650838423575 1104 30-31 0.22885487308922592 1104 32-33 0.21076369219092328 1104 34-35 0.30024813929940564 1104 36-37 -0.014236760246390645 1104 38-39 0.13883909446592924 1104 40-41 0.11477172407808212 1104 42-43 0.03396596162625798 1104 44-45 -0.19034199174343058 1104 46-47 -0.3451243040181353 1104 48-49 -0.4315984976793601 1104 50-51 -0.4579729262059118 1104 52-53 -0.3575458141416945 1104 54-55 -0.5476299740907322 1104 56-57 -0.5269980050639802 1104 58-59 -0.4419292742541998 1104 60-61 -0.5173426135482657 1104 62-63 -0.5129348312247046 1104 64-65 -0.43252639183939223 1104 66-67 -0.5171595091822923 1104 68-69 -0.479442907596475 1104 70-71 -0.48603212844454546 1104 72-73 -0.5714300217463197 1104 74-75 -0.603997314232771 1104 76-77 -0.5435267044682952 1104 78-79 -0.5006938350398933 1104 80-81 -0.5631372029229524 1104 82-83 -0.4446194977398541 1104 84-85 -0.4170827980277245 1104 86-87 -0.28589443887112154 1104 88-89 -0.10725746696958893 1104 90-91 -0.10704525416864996 1104 92-93 -0.20997689214201998 1104 94-95 -0.1260294712811323 1104 96-97 -0.09752969445241177 1104 98-99 0.1389553498259417 1104 100-101 1.0350409610378755 1105 1 0.027989666048618744 1105 2 0.015453829177104694 1105 3 -0.007477753479705029 1105 4 0.04974630413222769 1105 5 0.15917779211044802 1105 6 0.0402096714698672 1105 7 -0.08522494807653658 1105 8 -0.02886301843250294 1105 9 0.17626636542280494 1105 10-11 0.07197519298355814 1105 12-13 0.02616702534475479 1105 14-15 0.18125247412208978 1105 16-17 0.10232379844374151 1105 18-19 -0.06080950725198875 1105 20-21 0.02374347188261794 1105 22-23 0.03336057878827603 1105 24-25 -0.03511772477668984 1105 26-27 0.1630234601518552 1105 28-29 0.2717073930756939 1105 30-31 0.1912149143372659 1105 32-33 0.0634884855281257 1105 34-35 0.04562886654958476 1105 36-37 0.12284251825109749 1105 38-39 0.18360279168732063 1105 40-41 0.19267866806077194 1105 42-43 0.11671785920490407 1105 44-45 0.25079841498924793 1105 46-47 0.08673473138642862 1105 48-49 -0.053552933251843626 1105 50-51 0.3066512489742692 1105 52-53 0.19687848838898248 1105 54-55 0.19058644285251347 1105 56-57 0.16609238560901218 1105 58-59 0.2033691823254813 1105 60-61 0.12080674716354878 1105 62-63 0.10708173560895062 1105 64-65 0.26073135766332456 1105 66-67 0.3298559250208939 1105 68-69 0.5184349599026667 1105 70-71 0.2923853444934785 1105 72-73 0.15244537763270571 1105 74-75 0.3539234917944043 1105 76-77 0.21386497717801944 1105 78-79 0.411282182496155 1105 80-81 0.4501111925172232 1105 82-83 0.25863864621127775 1105 84-85 0.2292553118881422 1105 86-87 0.26782492798281154 1105 88-89 0.6545125921413266 1105 90-91 0.6777825690959141 1105 92-93 0.6612001982937308 1105 94-95 0.6326171198392672 1105 96-97 0.5489160435324081 1105 98-99 0.5462115102650209 1105 100-101 0.8789436337076708 1106 1 0.08077929144098661 1106 2 -0.03602335928638212 1106 3 0.01968199696771933 1106 4 0.015076559873442363 1106 5 0.057122161287693984 1106 6 0.04913134340523584 1106 7 0.07124273441854712 1106 8 0.11629005274796356 1106 9 0.16924031182140453 1106 10-11 0.05030046993896775 1106 12-13 0.0357365527225042 1106 14-15 0.07225609252297005 1106 16-17 -0.1061451552306849 1106 18-19 -0.19813590485235721 1106 20-21 -0.22665711687413648 1106 22-23 -0.18932105211253258 1106 24-25 -0.17081835088827546 1106 26-27 0.02274937604214955 1106 28-29 -0.047426643866565144 1106 30-31 -0.1085754525763889 1106 32-33 0.03580967017807524 1106 34-35 -0.08125880691342502 1106 36-37 0.06270068139693308 1106 38-39 0.1427550042641883 1106 40-41 0.16400353801577694 1106 42-43 0.35500265913353957 1106 44-45 0.2764321926778166 1106 46-47 0.17795523082610032 1106 48-49 0.26935784445345234 1106 50-51 0.3877023135869777 1106 52-53 0.13455446031152718 1106 54-55 0.058241061192063626 1106 56-57 -0.1593919727520614 1106 58-59 0.10114573352668543 1106 60-61 0.27366550687162317 1106 62-63 0.12908763432717052 1106 64-65 0.23769401955748748 1106 66-67 0.22298988390827645 1106 68-69 0.1121565604735828 1106 70-71 0.14912900671659202 1106 72-73 0.22899156102820584 1106 74-75 0.1650630371805235 1106 76-77 0.07405810723416195 1106 78-79 0.13888684571725918 1106 80-81 0.009782469996721943 1106 82-83 0.30956945056674456 1106 84-85 0.341703948994315 1106 86-87 0.4613313201193421 1106 88-89 0.44944683091118165 1106 90-91 0.3790481731012463 1106 92-93 0.6304214894328304 1106 94-95 0.738789587655571 1106 96-97 0.9190817986296693 1106 98-99 1.4030415903827702 1106 100-101 1.5816499076468205 1107 1 -0.1705975165921707 1107 2 -0.05035657795396986 1107 3 -0.07283360576460751 1107 4 -0.03403641865129714 1107 5 -0.07383267678788741 1107 6 -0.0972050859033331 1107 7 -0.1598480644741116 1107 8 -0.06482613641775714 1107 9 -0.17131041139185754 1107 10-11 -0.13193289124175323 1107 12-13 -0.0473273160998815 1107 14-15 -0.15568467442708567 1107 16-17 -0.1788776400374772 1107 18-19 -0.10274219181158628 1107 20-21 -0.2191432341280546 1107 22-23 -0.32817636031768416 1107 24-25 -0.4188272620550748 1107 26-27 -0.2649486271357304 1107 28-29 -0.21703530604488463 1107 30-31 -0.24633979808067608 1107 32-33 -0.07605495871441548 1107 34-35 -0.26753495479947986 1107 36-37 -0.34292613073142775 1107 38-39 -0.12186078357140673 1107 40-41 -0.16078883507380937 1107 42-43 -0.28565126325413104 1107 44-45 -0.2699648456696835 1107 46-47 -0.3105994893075632 1107 48-49 -0.4986029070986291 1107 50-51 -0.39403634678945565 1107 52-53 -0.2928211683024955 1107 54-55 -0.3193909272758404 1107 56-57 -0.3830163932805064 1107 58-59 -0.3797001720870199 1107 60-61 -0.29793178701669376 1107 62-63 -0.18856106257849348 1107 64-65 -0.3209493208195937 1107 66-67 -0.5014732346724884 1107 68-69 -0.5412498313228973 1107 70-71 -0.4677940198039572 1107 72-73 -0.3905687777271787 1107 74-75 -0.2277425060894167 1107 76-77 -0.2700488922907951 1107 78-79 -0.20629473568723355 1107 80-81 -0.4125789892068461 1107 82-83 -0.2389341614043623 1107 84-85 -0.279846659553197 1107 86-87 -0.4063935476055285 1107 88-89 0.09923469927490203 1107 90-91 0.1205276888841702 1107 92-93 -0.2155496475599783 1107 94-95 0.08239533404769261 1107 96-97 0.013278073630900167 1107 98-99 -0.14834176997891646 1107 100-101 -0.005769621568372685 1108 1 -0.13461624346674483 1108 2 -0.18891405486000679 1108 3 -0.1373834308294164 1108 4 -0.059323362697206505 1108 5 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#Quality Count 3 6.0 4 28.0 5 71.0 6 252.0 7 495.0 8 781.0 9 1193.0 10 1979.0 11 3305.0 12 5123.0 13 7228.0 14 6744.0 15 8099.0 16 10359.0 17 14173.0 18 13044.0 19 8045.0 20 6862.0 21 7027.0 22 7384.0 23 8251.0 24 9464.0 25 10668.0 26 12716.0 27 15314.0 28 18376.0 29 21750.0 30 26476.0 31 32069.0 32 39014.0 33 49126.0 34 67810.0 35 106743.0 36 124989.0 37 175197.0 38 133086.0 39 15302.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.37555891370186 17.153701527127396 12.594706981005743 16.876032578165002 2 12.93659110737129 25.277552642854562 40.781060445715525 21.004795804058624 3 23.692923995023516 33.41462281439877 28.906050766347807 13.98640242422991 4 10.44824506610363 17.069552064673662 39.627176567154166 32.855026302068545 5 10.499765114010046 43.88929900728405 31.7830169685769 13.827918910129005 6 21.823128268399834 36.46855367015812 22.992395888720825 18.715922172721218 7 24.84825332583075 38.183995746240534 21.36605561893749 15.601695308991228 8 22.468625942770416 31.17726923096778 21.925961871034687 24.428142955227116 9 27.444698545771796 21.568831919715453 23.274465569843734 27.71200396466902 10-11 22.09962263371724 26.66838744314985 28.36885876382859 22.863131159304327 12-13 31.77594457723103 24.08949414333724 26.994130431415876 17.140430848015857 14-15 26.77449079941459 25.293208163728863 31.489413325555404 16.442887711301143 16-17 17.550803585625285 29.124326043277776 34.558208591541444 18.766661779555495 18-19 16.45545834305573 32.228765768351586 34.85236754997656 16.463408338616123 20-21 18.995100949352388 29.9674257011734 31.111810893547602 19.925662455926613 22-23 17.83419630273965 28.682573524477185 29.28692743313493 24.19630273964824 24-25 19.21842332133952 29.28646282348447 32.0551233538622 19.439990501313815 26-27 22.67389876847087 25.080429341945504 34.443633382616525 17.8020385069671 28-29 17.986330151617615 26.5058773120783 37.38548662698556 18.12230590931852 30-31 17.35559673947272 32.02229093856312 34.05924289276293 16.562869429201232 32-33 17.365250295543362 30.92622514983661 32.114593389120905 19.59393116549912 34-35 19.303425616540764 27.230481544046885 32.56762226526844 20.898470574143907 36-37 21.906192880820345 27.111692844677137 34.438592686679684 16.543521587822838 38-39 23.592391006915296 24.560552975786656 35.11822707404642 16.728828943251624 40-41 18.56494926262368 27.55802337197686 37.589697124580844 16.287330240818612 42-43 17.168663969408822 31.364269714082756 33.56759243150206 17.899473885006365 44-45 17.43982249397412 32.145864275828174 30.51163592679838 19.902677303399326 46-47 17.876404244961343 29.435442030321006 28.779400673077372 23.908753051640282 48-49 20.139186798471332 29.25178707031974 29.787585520484395 20.82144061072454 50-51 22.45118465992393 30.416148709201817 30.447329404428068 16.685337226446183 52-53 18.310885804110246 30.613800971550404 33.00576632199514 18.069546902344214 54-55 15.886552648016892 30.1473328824899 34.151338850142736 19.814775619350474 56-57 19.17392404148448 32.53594271879243 29.317178683709237 18.972954556013853 58-59 18.337603446789466 30.03112373245257 30.402760405078293 21.22851241567967 60-61 18.53177051202961 27.815672437335287 32.54873922032734 21.103817830307754 62-63 23.842115316115244 26.641388460128752 30.80614936684029 18.710346856915713 64-65 21.59841206746132 28.40107169959373 32.79586600657681 17.204650226368145 66-67 17.774199660004246 29.173040081400746 34.05285163309987 18.999908625495138 68-69 16.841756939253397 33.06552695374933 31.568182475210314 18.524533631786962 70-71 17.608556041514376 32.47548867771018 28.48016379427835 21.43579148649709 72-73 17.931867575924958 30.08553979897889 27.71076500560113 24.27182761949502 74-75 18.67539788654232 31.047075282250365 29.524859197464266 20.752667633743048 76-77 21.211626404857252 27.658597080480558 31.526727812944067 19.603048701718123 78-79 18.33046373826814 27.90186294731341 33.99137778778493 19.776295526633525 80-81 17.187322544741956 32.827751472814114 31.356331190620246 18.628594791823687 82-83 17.28058809257089 32.02182632891265 29.09555988044045 21.602025698075998 84-85 18.50209848692124 28.812870719783596 29.47633330063858 23.208697492656587 86-87 20.45572403010517 28.58682844635352 31.17307679382138 19.784370729719935 88-89 21.954696157206914 25.912372653190396 32.07324154055649 20.059689649046202 90-91 18.202018926007575 27.960230326136593 34.30817769613807 19.52957305171776 92-93 16.822895963574602 31.5874163057338 31.680854468770487 19.90883326192111 94-95 16.41115703865916 32.697127523541525 29.464836516685434 21.426878921113886 96-97 16.756517057119655 29.999499253784894 27.762505878605698 25.481477810489757 98-99 18.86819822973033 29.482743845716236 28.293443931307838 23.355613993245594 100-101 20.908368179237 29.623612616798304 29.593051468690312 19.874967735274378 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8616.0 1 5582.5 2 8048.5 3 10364.0 4 5226.5 5 3580.5 6 5146.5 7 8458.0 8 10262.0 9 9169.5 10 6686.0 11 4517.0 12 4013.5 13 4049.0 14 4117.5 15 4014.5 16 3676.5 17 3619.0 18 3779.5 19 3895.0 20 4035.5 21 4283.0 22 4588.0 23 5020.5 24 5657.5 25 6412.5 26 7543.0 27 8775.5 28 9624.0 29 10637.0 30 11826.0 31 12918.0 32 14355.5 33 16016.0 34 17774.5 35 18733.5 36 19589.0 37 20814.5 38 20780.5 39 20025.5 40 19087.5 41 18672.5 42 18487.5 43 18652.0 44 22006.5 45 24715.5 46 29539.5 47 47279.5 48 117156.0 49 115180.0 50 50925.5 51 34569.0 52 18632.0 53 12500.0 54 10402.5 55 9105.5 56 7996.0 57 6919.5 58 6223.0 59 5697.5 60 5168.5 61 4552.0 62 3710.0 63 2491.0 64 1442.0 65 1074.5 66 847.5 67 639.0 68 523.0 69 458.0 70 376.0 71 299.5 72 270.5 73 286.5 74 243.0 75 177.5 76 123.0 77 68.5 78 45.0 79 31.5 80 22.0 81 18.5 82 11.0 83 4.5 84 1.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38583250305868017 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.5811647247704053E-4 16-17 0.0021681783688071404 18-19 1.0324658899081621E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.0973976697244867E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00898245324220101 36-37 0.019100618963301 38-39 0.012596083856879577 40-41 0.009034076536696418 42-43 0.004181486854128056 44-45 0.0025295414302749972 46-47 0.0019100618963301 48-49 5.1623294495408105E-5 50-51 7.227261229357135E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.5486988348622433E-4 60-61 7.743494174311217E-4 62-63 0.0 64-65 0.0 66-67 0.001393828951376019 68-69 7.227261229357135E-4 70-71 0.002374671546788773 72-73 0.0 74-75 0.0 76-77 0.09601932776145908 78-79 0.03696227885871221 80-81 1.0324658899081621E-4 82-83 0.0 84-85 0.0 86-87 0.15265008182292178 88-89 0.3333316125568502 90-91 0.2884709696403405 92-93 0.0 94-95 5.1623294495408105E-5 96-97 5.1623294495408105E-5 98-99 2.0649317798163242E-4 100-101 5.162329449540811E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 968555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.77913073430337 #Duplication Level Percentage of deduplicated Percentage of total 1 83.89253133898438 35.888495657820556 2 10.092868253866625 8.635282610325213 3 2.576404578300128 3.3064904483867674 4 1.045421531417218 1.7888889745981122 5 0.544431810893511 1.164515980706352 6 0.3146364842587379 0.807592517633168 7 0.24246017452207386 0.7260564852619267 8 0.16648533535160848 0.5697678341080638 9 0.12617815185267978 0.4858012488526714 >10 0.841249879241994 6.838905249244842 >50 0.0785458551250449 2.316525616500826 >100 0.05493378046019468 4.783423211479064 >500 0.00891469244297779 2.6476884969639505 >1k 0.01108313114532374 10.322872393196453 >5k 0.0024093763359399434 7.669927273944076 >10k+ 0.001445625801563966 12.047766000977976 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 33017 3.4088926287097787 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 30668 3.166366391170352 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 17540 1.8109451708989164 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13979 1.44328406750262 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11253 1.161833865913655 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10431 1.0769651697632039 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9784 1.010164626686146 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9304 0.960606263970554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9290 0.9591608117246827 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7816 0.8069753395522196 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7530 0.7774468151008461 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6822 0.7043482300953482 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 6780 0.700011873357734 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6122 0.6320756178017768 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5581 0.5762192131577453 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5385 0.5559828817155453 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4831 0.49878427141463316 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4723 0.487633639803625 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4373 0.4514973336568393 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4240 0.43776553732106077 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4172 0.4307447692696853 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3839 0.3963636551357435 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3471 0.3583689103871231 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3417 0.352793594581619 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3266 0.3372033596440058 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2961 0.3057131500018068 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2739 0.28279240724584565 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2733 0.2821729277119007 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2408 0.24861778628988543 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2364 0.24407493637428954 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2163 0.22332237198713548 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2161 0.22311587880915382 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2129 0.21981198796144774 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 2063 0.21299771308805385 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2015 0.20804187681649466 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1958 0.20215682124401813 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1888 0.19492956001466102 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1859 0.19193540893392735 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1858 0.19183216234493652 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1853 0.19131592939998246 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1774 0.18315944886970797 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1712 0.17675816035227737 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1666 0.17200881725869982 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1620 0.16725947416512227 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1587 0.16385233672842534 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1535 0.1584835141009029 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1534 0.1583802675119121 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1503 0.15517962325319676 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1487 0.15352767782934373 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1464 0.15115300628255493 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1448 0.14950106085870188 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1442 0.14888158132475698 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1391 0.14361600528622537 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1276 0.1317426475522815 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1272 0.13132966119631823 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1266 0.13071018166237333 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1184 0.1222439613651264 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1154 0.11914656369540191 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1151 0.11883682392842947 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1140 0.1177011114495305 No Hit GTACCAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1035 0.10686021960549477 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1028 0.10613749348255908 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.5811647247704053E-4 0.0 14-15 0.0 0.0 0.0 3.0973976697244867E-4 0.0 16-17 0.0 0.0 0.0 5.678562394494891E-4 0.0 18-19 0.0 0.0 0.0 8.775960064219379E-4 0.0 20-21 0.0 0.0 0.0 0.001445452245871427 0.0 22-23 0.0 0.0 0.0 0.003716877203669384 0.0 24-25 0.0 0.0 0.0 0.008259727119265297 0.0 26-27 0.0 0.0 0.0 0.012183097500916314 0.0 28-29 0.0 0.0 0.0 0.01884250249082396 0.0 30-31 0.0 1.0324658899081621E-4 0.0 0.032884038593574964 0.0 32-33 0.0 1.0324658899081621E-4 0.0 0.06019276138164586 0.0 34-35 0.0 1.0324658899081621E-4 0.0 0.12007578299631927 0.0 36-37 0.0 1.0324658899081621E-4 0.0 0.21051979495227424 0.0 38-39 0.0 1.0324658899081621E-4 0.0 0.307881328370614 0.0 40-41 0.0 1.0324658899081621E-4 0.0 0.4164451166944572 0.0 42-43 0.0 1.0324658899081621E-4 0.0 0.5181946301449065 0.0 44-45 0.0 1.0324658899081621E-4 0.0 0.6348632757045289 0.0 46-47 0.0 1.0324658899081621E-4 0.0 0.7835383638513043 0.0 48-49 0.0 1.0324658899081621E-4 0.0 0.9636004150512878 0.0 50-51 0.0 1.0324658899081621E-4 0.0 1.1316342386338412 0.0 52-53 0.0 1.0324658899081621E-4 0.0 1.2966222878411653 0.0 54-55 0.0 1.0324658899081621E-4 0.0 1.478078167992525 0.0 56-57 0.0 1.0324658899081621E-4 0.0 1.686946017520946 0.0 58-59 0.0 1.0324658899081621E-4 0.0 1.9831604813355979 0.0 60-61 0.0 1.0324658899081621E-4 0.0 2.38695789087868 0.0 62-63 0.0 1.0324658899081621E-4 0.0 2.7271554016034196 0.0 64-65 0.0 1.0324658899081621E-4 0.0 3.0166072138391726 0.0 66-67 0.0 1.0324658899081621E-4 0.0 3.3271213302290525 0.0 68-69 0.0 1.0324658899081621E-4 0.0 3.6650990392904896 0.0 70-71 0.0 1.0324658899081621E-4 0.0 4.064973078451921 0.0 72-73 0.0 1.0324658899081621E-4 0.0 4.497524663028946 0.0 74-75 0.0 1.0324658899081621E-4 0.0 4.906794141788541 0.0 76-77 0.0 1.0324658899081621E-4 0.0 5.289632493766487 0.0 78-79 0.0 1.0324658899081621E-4 0.0 5.717744475016906 0.0 80-81 0.0 1.0324658899081621E-4 0.0 6.20393266257466 0.0 82-83 0.0 1.0324658899081621E-4 0.0 6.708808482739752 0.0 84-85 0.0 1.0324658899081621E-4 0.0 7.44160114810207 0.0 86-87 0.0 1.0324658899081621E-4 0.0 8.249195967188234 0.0 88-89 0.0 1.0324658899081621E-4 0.0 8.835326852889096 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1720 0.0 58.210754 2 ACCTGGG 1755 0.0 54.88683 3 TATCACG 45 1.5249991E-5 52.7238 2 ATAACGC 100 1.2732926E-11 47.451424 3 TATAACG 100 1.2732926E-11 47.451424 2 TAACGCA 105 2.0008883E-11 45.191833 4 CCTGGGA 1265 0.0 44.262985 4 AGTACTC 805 0.0 41.262108 5 AAGTACT 815 0.0 39.59137 4 GTACCAG 350 0.0 39.475845 1 ACACGGG 145 0.0 39.27014 3 CCTGGGG 760 0.0 38.710373 4 GTACCCG 75 6.779561E-6 38.11461 1 CATTCCG 90 5.7402394E-7 36.908566 9 GTACCTG 2735 0.0 36.755863 1 AAAGTAC 910 0.0 34.93676 3 GTACACG 165 1.8189894E-12 34.649647 1 ATCACGC 85 1.6434067E-5 33.495125 3 TACCAGC 445 0.0 31.989723 2 TCACGCA 80 4.5201273E-4 29.65714 4 >>END_MODULE