##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938700_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 968725 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99234044749542 31.0 31.0 34.0 30.0 34.0 2 32.09422178636868 33.0 31.0 34.0 30.0 34.0 3 32.18799865802988 34.0 31.0 34.0 30.0 34.0 4 35.772930398203826 37.0 35.0 37.0 35.0 37.0 5 35.725785955766604 37.0 35.0 37.0 33.0 37.0 6 35.817684069266306 37.0 35.0 37.0 35.0 37.0 7 35.78180391752045 37.0 35.0 37.0 33.0 37.0 8 35.813166791401066 37.0 35.0 37.0 33.0 37.0 9 37.40180959508633 39.0 37.0 39.0 34.0 39.0 10-11 37.30272316704948 39.0 37.0 39.0 33.5 39.0 12-13 37.210819375983895 39.0 37.0 39.0 33.0 39.0 14-15 38.171973986425456 40.0 37.0 41.0 33.0 41.0 16-17 38.13625177423934 40.0 37.5 41.0 33.0 41.0 18-19 38.48496270871506 40.0 38.0 41.0 34.0 41.0 20-21 38.48027871687012 40.0 38.0 41.0 34.0 41.0 22-23 38.35200960024775 40.0 37.5 41.0 34.0 41.0 24-25 38.21789207463418 40.0 37.0 41.0 33.5 41.0 26-27 37.80442592066892 40.0 36.5 41.0 32.5 41.0 28-29 37.532948463186145 39.5 36.0 41.0 32.5 41.0 30-31 37.302362383545386 39.0 35.5 41.0 32.5 41.0 32-33 37.01372835428011 39.0 35.0 41.0 32.0 41.0 34-35 36.86946347002503 39.0 35.0 41.0 31.0 41.0 36-37 36.55613615835247 39.0 35.0 41.0 31.0 41.0 38-39 36.281580427882005 39.0 35.0 41.0 30.0 41.0 40-41 36.02202895558595 38.5 35.0 40.5 30.0 41.0 42-43 35.90238354538182 39.0 35.0 41.0 30.0 41.0 44-45 35.64265555240135 39.0 35.0 41.0 29.0 41.0 46-47 35.14402023278021 38.0 35.0 40.5 25.5 41.0 48-49 34.73359106041447 38.0 35.0 40.0 23.5 41.0 50-51 34.49718289504246 38.0 34.0 40.0 23.0 41.0 52-53 34.06917907558905 38.0 34.0 40.0 22.0 41.0 54-55 33.856130996928954 38.0 33.5 40.0 22.0 41.0 56-57 33.76359751219386 38.0 33.0 40.0 20.5 41.0 58-59 33.574418436604816 38.0 33.0 40.0 20.0 41.0 60-61 33.13781775013548 37.0 33.0 40.0 16.5 41.0 62-63 32.60771271516684 36.0 32.0 39.0 13.5 41.0 64-65 32.03984722186379 36.0 31.0 39.0 8.5 40.0 66-67 31.646632429223978 35.0 31.0 39.0 8.0 40.0 68-69 31.476734367338512 35.0 31.0 38.0 6.5 40.0 70-71 31.037388835840922 35.0 31.0 37.5 2.0 39.5 72-73 30.431079511729337 35.0 30.0 37.0 2.0 39.0 74-75 29.947435030581435 34.5 29.5 36.0 2.0 39.0 76-77 27.898596092802393 32.0 26.5 34.5 2.0 36.0 78-79 28.775006322743813 34.0 29.0 35.0 2.0 37.0 80-81 28.93588221631526 34.0 29.0 35.0 2.0 37.0 82-83 28.675861570621176 34.0 29.0 35.0 2.0 36.0 84-85 28.332116441714625 34.0 29.0 35.0 2.0 36.0 86-87 27.624817672714137 34.0 27.0 35.0 2.0 35.5 88-89 26.851349454179463 33.0 25.5 35.0 2.0 35.0 90-91 26.212681617590132 32.5 24.5 35.0 2.0 35.0 92-93 26.02360164133268 32.5 24.0 35.0 2.0 35.0 94-95 25.78241915920411 33.0 23.5 35.0 2.0 35.0 96-97 24.990460657049212 32.0 19.0 34.5 2.0 35.0 98-99 24.1093174017394 32.0 2.0 34.0 2.0 35.0 100-101 20.62266174610958 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.010812004560907695 1101 2 0.16461921657257506 1101 3 0.04185262904050546 1101 4 0.05291142231234147 1101 5 -0.08648354116036927 1101 6 0.12051620817864261 1101 7 -0.03611154735566657 1101 8 0.06964281619720936 1101 9 0.10940852777064691 1101 10-11 0.09784835281854498 1101 12-13 0.08311952319781568 1101 14-15 0.31581858621518677 1101 16-17 0.3541183066316762 1101 18-19 0.3973247551002075 1101 20-21 0.32483310953575995 1101 22-23 0.24425448268168992 1101 24-25 0.34873261526690413 1101 26-27 0.30151335455305883 1101 28-29 0.2517123973614517 1101 30-31 0.13991894384676584 1101 32-33 0.27758349530893867 1101 34-35 0.45167911324763566 1101 36-37 0.5190044006557741 1101 38-39 0.4872285160069225 1101 40-41 0.345979272826078 1101 42-43 0.39494163582130426 1101 44-45 0.2829236900099943 1101 46-47 0.1324101294380995 1101 48-49 0.2398981439781025 1101 50-51 0.13005160015546124 1101 52-53 0.10548706775306727 1101 54-55 0.10309080344951127 1101 56-57 0.008533633913053507 1101 58-59 -0.10172885145198052 1101 60-61 0.11761185514217942 1101 62-63 0.12394083055080074 1101 64-65 0.06788632976734021 1101 66-67 0.1440544706385971 1101 68-69 0.0947920053222262 1101 70-71 0.05858943549865714 1101 72-73 0.041367594494460036 1101 74-75 0.001893102640377009 1101 76-77 0.04374051631234366 1101 78-79 -0.18064467144534646 1101 80-81 0.19271009954663043 1101 82-83 0.33028104488039745 1101 84-85 0.3743848527121152 1101 86-87 0.2360963736850934 1101 88-89 0.1720297107159574 1101 90-91 0.12644876082551804 1101 92-93 0.05823715128328999 1101 94-95 0.06650152169509482 1101 96-97 0.01574361932706836 1101 98-99 -0.10692444903940412 1101 100-101 0.25776939973690816 1102 1 0.23286371373560044 1102 2 0.13824871795307558 1102 3 0.10737008148812777 1102 4 0.021520626081986904 1102 5 -0.0022315141318642873 1102 6 -0.033554353618129085 1102 7 0.08408484869804767 1102 8 0.13280915604720178 1102 9 0.34970294696604043 1102 10-11 0.14532148061807248 1102 12-13 0.23591670229991024 1102 14-15 0.25958644363129935 1102 16-17 0.2399450653753945 1102 18-19 0.3713165967067056 1102 20-21 0.3624060967713305 1102 22-23 0.3199081706821616 1102 24-25 0.47502539928589016 1102 26-27 0.34586532846019225 1102 28-29 0.3519325340779389 1102 30-31 0.3451915133253678 1102 32-33 0.5773951658340124 1102 34-35 0.6111230221062272 1102 36-37 0.6515919580126521 1102 38-39 0.5860184198856544 1102 40-41 0.5958967488831988 1102 42-43 0.61446133554346 1102 44-45 0.664366842490189 1102 46-47 0.7804848622463325 1102 48-49 0.8943321390332244 1102 50-51 0.9286767840581547 1102 52-53 0.8048020643755862 1102 54-55 0.8790765546797203 1102 56-57 0.7547391720893941 1102 58-59 0.9150562999220782 1102 60-61 0.933791138681336 1102 62-63 0.7732741901169149 1102 64-65 0.7523819937079921 1102 66-67 1.036586436369845 1102 68-69 0.9306261966859637 1102 70-71 0.8848748962008663 1102 72-73 0.8803743122713215 1102 74-75 0.7769699974666189 1102 76-77 0.6273820847700513 1102 78-79 0.9292777669786751 1102 80-81 1.1169022939839692 1102 82-83 1.156097594701908 1102 84-85 1.3429290210565874 1102 86-87 1.5469411128110622 1102 88-89 1.593678418861014 1102 90-91 1.436796716745711 1102 92-93 1.8214715870490572 1102 94-95 1.9237296353617168 1102 96-97 1.6020653031215524 1102 98-99 1.9898602869823492 1102 100-101 2.4514869776646506 1103 1 0.15465081192173002 1103 2 0.12280696406850211 1103 3 0.20419054347485144 1103 4 0.05211555306782856 1103 5 -0.10143035380110632 1103 6 0.12039973950871996 1103 7 0.0724954873519934 1103 8 0.1355640833763445 1103 9 0.20693162739017623 1103 10-11 0.25823541703159947 1103 12-13 0.22223164370406323 1103 14-15 0.2913989884203261 1103 16-17 0.21422972832591824 1103 18-19 0.23684063366220443 1103 20-21 0.22423329588563234 1103 22-23 0.2739734049582623 1103 24-25 0.24596842550061382 1103 26-27 0.14649355487916438 1103 28-29 0.14523091587997072 1103 30-31 0.4066710208713715 1103 32-33 0.3111944123056034 1103 34-35 0.30637474179022206 1103 36-37 0.32378349841180665 1103 38-39 0.28451479599760177 1103 40-41 0.49661208593268213 1103 42-43 0.3874002894434838 1103 44-45 0.4928972904448088 1103 46-47 0.5918887380257232 1103 48-49 0.3627046506944609 1103 50-51 0.2543042595234226 1103 52-53 0.21959724711481243 1103 54-55 0.14950356841573154 1103 56-57 -7.256253462060158E-4 1103 58-59 0.17280721501280283 1103 60-61 0.4142963803382358 1103 62-63 0.36402158216194636 1103 64-65 0.519998294982031 1103 66-67 0.6825085431597557 1103 68-69 0.6080985508614702 1103 70-71 0.5697704278117257 1103 72-73 0.6791112081290613 1103 74-75 0.7421970858688915 1103 76-77 0.8320781201635761 1103 78-79 0.5625613228088646 1103 80-81 0.7142956263732465 1103 82-83 0.733932337682635 1103 84-85 0.7116295922105245 1103 86-87 0.944594704300826 1103 88-89 0.9122560481645152 1103 90-91 0.5855488462358736 1103 92-93 0.7248941122274637 1103 94-95 0.9601170974385553 1103 96-97 0.8698763159650049 1103 98-99 0.9608117882460974 1103 100-101 1.1625950849609161 1104 1 0.04545802939561483 1104 2 0.11803963263450612 1104 3 0.02656154695696955 1104 4 0.07933619251069501 1104 5 -0.040790910939442426 1104 6 0.08465425042989239 1104 7 0.18165977984961756 1104 8 0.03930577378469735 1104 9 0.27172428329394904 1104 10-11 0.20216862374259392 1104 12-13 0.1019261672874805 1104 14-15 0.17408338221583364 1104 16-17 0.3577419157269901 1104 18-19 0.30742151368752957 1104 20-21 0.2292807792664533 1104 22-23 0.23001645655686787 1104 24-25 0.23117576959685238 1104 26-27 0.21950400122295122 1104 28-29 0.06521667086572336 1104 30-31 -0.12059710195229911 1104 32-33 0.12094908150471184 1104 34-35 -0.12441506511069633 1104 36-37 -0.21685594444985412 1104 38-39 -0.07666279184665115 1104 40-41 -0.08241292198120931 1104 42-43 0.09471747842733436 1104 44-45 0.08782289981486002 1104 46-47 0.2346823435971075 1104 48-49 -0.00940519419316388 1104 50-51 0.00841315474342963 1104 52-53 0.08179572764662879 1104 54-55 0.06741429965979506 1104 56-57 0.2297302151096332 1104 58-59 0.35509907952689446 1104 60-61 0.19925997641294657 1104 62-63 0.05325868251016885 1104 64-65 -0.06738378795560607 1104 66-67 -0.0980580918513212 1104 68-69 -0.113748552652293 1104 70-71 -8.848428435435096E-4 1104 72-73 0.15208683332690498 1104 74-75 0.13600050467230673 1104 76-77 0.14106514137281678 1104 78-79 0.062479018282115106 1104 80-81 -0.17081675096135385 1104 82-83 -0.126822648072352 1104 84-85 -0.20858724856187294 1104 86-87 -0.058241636313294265 1104 88-89 -0.0019019132156685714 1104 90-91 -0.051890216758742724 1104 92-93 -0.32416729961172663 1104 94-95 -0.12996678986755938 1104 96-97 -0.1903215314550657 1104 98-99 0.039977663523082185 1104 100-101 0.6625444360213777 1105 1 0.1928323571954742 1105 2 0.13472110718831942 1105 3 0.14143334182876544 1105 4 0.06194393937376219 1105 5 -0.1443713021794082 1105 6 -0.06831278253714146 1105 7 0.021001997915242043 1105 8 0.11301164238418693 1105 9 0.021383701889533313 1105 10-11 0.11707471056357122 1105 12-13 0.15233377386529412 1105 14-15 0.0032393326483841633 1105 16-17 0.05883146447611409 1105 18-19 0.1356063784681396 1105 20-21 0.03487816060851401 1105 22-23 -0.016594096011125714 1105 24-25 0.13339071776924527 1105 26-27 0.013693074560954699 1105 28-29 0.08437028321083062 1105 30-31 -0.09579311161640192 1105 32-33 0.09443049370953105 1105 34-35 0.26625284853594167 1105 36-37 0.32011857039700686 1105 38-39 0.24729013465947247 1105 40-41 0.1883578675129769 1105 42-43 0.4417785025096421 1105 44-45 0.34979936306792325 1105 46-47 0.30072240282865437 1105 48-49 0.2167720328351308 1105 50-51 0.29562225769721096 1105 52-53 0.43106390627650626 1105 54-55 0.32206163813692257 1105 56-57 0.10005676798937913 1105 58-59 0.25504920032808087 1105 60-61 0.30760694858970794 1105 62-63 0.22575498053837606 1105 64-65 0.1243674395403005 1105 66-67 0.32763281298851865 1105 68-69 0.19674634252345058 1105 70-71 0.3823900383036758 1105 72-73 0.708246135018122 1105 74-75 0.31240140873278577 1105 76-77 0.38558544376546067 1105 78-79 0.21058438808961455 1105 80-81 0.3344345934389281 1105 82-83 0.4044687555594777 1105 84-85 0.5950570504015751 1105 86-87 0.529876203293906 1105 88-89 0.5069672318236904 1105 90-91 0.4826963314448278 1105 92-93 0.47952999600046553 1105 94-95 0.7091961748273228 1105 96-97 0.9063864414231197 1105 98-99 0.7885054477955507 1105 100-101 1.5463368138563105 1106 1 0.14361045667847705 1106 2 0.05201449834849825 1106 3 -0.025892086366226863 1106 4 0.16199860256440957 1106 5 0.05467579626640173 1106 6 0.09270735674387254 1106 7 0.11380227503559581 1106 8 0.20174521839805237 1106 9 -0.0039005266786915627 1106 10-11 0.13847094461012688 1106 12-13 0.00948013505882983 1106 14-15 0.014288105029244491 1106 16-17 0.11952462577056622 1106 18-19 0.0934461412338976 1106 20-21 0.07382217989481177 1106 22-23 0.01826820480862068 1106 24-25 0.11407989445749678 1106 26-27 0.09112708828082106 1106 28-29 0.08164212946509508 1106 30-31 -0.024474166916320428 1106 32-33 0.11436704035745748 1106 34-35 0.01839632639634914 1106 36-37 -0.10010921292050767 1106 38-39 0.017246704454151995 1106 40-41 0.03795750469355852 1106 42-43 -0.09485584679816128 1106 44-45 0.09124997998107176 1106 46-47 -0.019622916360326315 1106 48-49 0.0380063797401462 1106 50-51 0.13630258415459906 1106 52-53 -0.07627207411682235 1106 54-55 0.12134761272354666 1106 56-57 0.19864123162934533 1106 58-59 0.09305843900799715 1106 60-61 0.1567470545522056 1106 62-63 0.22032820718838053 1106 64-65 0.2179886656777157 1106 66-67 0.08316771133100076 1106 68-69 0.021623900981026623 1106 70-71 0.27610917762873655 1106 72-73 0.18587977951115775 1106 74-75 0.4672739413805296 1106 76-77 0.21465184452474162 1106 78-79 0.06463316847974454 1106 80-81 -0.08507285717398005 1106 82-83 0.02792296862978816 1106 84-85 0.14807333722914606 1106 86-87 0.09574184397453323 1106 88-89 0.2954377036891671 1106 90-91 0.07628552054742954 1106 92-93 0.1303963975606699 1106 94-95 0.3661541182098702 1106 96-97 0.3437840730853239 1106 98-99 0.44136815708748856 1106 100-101 0.8671486723647455 1107 1 0.05655472386579774 1107 2 0.14270135645285364 1107 3 0.0018751088970390128 1107 4 0.11468682581313772 1107 5 0.03108737142076734 1107 6 0.08142904190348332 1107 7 0.061934725787217815 1107 8 -0.036940750775080744 1107 9 0.030609053142569564 1107 10-11 0.018957316977065375 1107 12-13 0.14453815369463996 1107 14-15 0.010714522160220952 1107 16-17 0.062517942912649 1107 18-19 0.014482063579407622 1107 20-21 -0.010836613381059124 1107 22-23 -0.12197458453055532 1107 24-25 -0.11786964282205759 1107 26-27 0.007418737089487593 1107 28-29 0.043713736049539875 1107 30-31 0.09975198671003938 1107 32-33 0.20033740402556788 1107 34-35 0.17518950702400105 1107 36-37 0.13002079327025484 1107 38-39 0.10511199962693496 1107 40-41 -0.03591307644522601 1107 42-43 -0.029192005662871168 1107 44-45 0.19864001442715562 1107 46-47 0.11979046840402674 1107 48-49 0.06254723089178782 1107 50-51 -0.04283491445644927 1107 52-53 -0.14220180092921453 1107 54-55 0.1652703392025856 1107 56-57 0.3632859766172061 1107 58-59 0.41816519012312625 1107 60-61 0.2513606869473932 1107 62-63 0.2255169463346931 1107 64-65 0.34622294667522624 1107 66-67 0.2322879075308215 1107 68-69 0.025360190010665917 1107 70-71 0.016948027097758 1107 72-73 0.19538618457806578 1107 74-75 0.2318222532048999 1107 76-77 0.4225387280271278 1107 78-79 0.5533826327399467 1107 80-81 0.352973795841244 1107 82-83 0.39368244923997153 1107 84-85 0.2616382688925576 1107 86-87 0.3008964545115447 1107 88-89 0.40730414177351904 1107 90-91 0.4454638147519141 1107 92-93 0.534955344812122 1107 94-95 0.4418898675312697 1107 96-97 0.5600939767315296 1107 98-99 1.0571911327847445 1107 100-101 1.642620123386223 1108 1 0.15468484080544798 1108 2 0.17357125279677632 1108 3 0.05176042745904397 1108 4 -0.03245189444828611 1108 5 0.0966495097203861 1108 6 0.09039987562425011 1108 7 0.19771157076359458 1108 8 0.08571857450841236 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-1.1329212739095667 2316 58-59 -1.0742650690300835 2316 60-61 -1.0477217414207871 2316 62-63 -1.1850418809025065 2316 64-65 -1.0261143847131073 2316 66-67 -1.0741440985202892 2316 68-69 -1.1114926023245957 2316 70-71 -0.8952852107865148 2316 72-73 -1.107547523347808 2316 74-75 -1.389031115426576 2316 76-77 -1.2120129315258836 2316 78-79 -1.3930366767851474 2316 80-81 -1.4297382181339415 2316 82-83 -1.4036652374709924 2316 84-85 -1.5148974704774751 2316 86-87 -1.7497122583668734 2316 88-89 -2.005972282308459 2316 90-91 -2.438050961843256 2316 92-93 -2.24442945897702 2316 94-95 -2.1965184743564627 2316 96-97 -2.034483886290065 2316 98-99 -2.2099797930711453 2316 100-101 -2.2930287501347664 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 19.0 5 87.0 6 262.0 7 482.0 8 716.0 9 1198.0 10 2045.0 11 3348.0 12 5198.0 13 7234.0 14 6812.0 15 8077.0 16 10388.0 17 14437.0 18 13334.0 19 8278.0 20 7334.0 21 7460.0 22 8003.0 23 8749.0 24 9851.0 25 11463.0 26 13347.0 27 15972.0 28 19171.0 29 23093.0 30 27557.0 31 32766.0 32 39991.0 33 50973.0 34 70739.0 35 109673.0 36 127944.0 37 169064.0 38 121430.0 39 12219.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.44530006684478 17.18831809837979 12.530188800291386 16.836193034484044 2 12.867635293814034 25.246380551756175 40.800330331105314 21.085653823324474 3 23.624145139229398 33.35600918733387 29.021961857080182 13.997883816356552 4 10.476347776716818 17.031252419417275 39.695372783813774 32.797027020052134 5 10.503218558391485 43.929759635539824 31.78339318867547 13.783628617393227 6 21.834042201759093 36.27431143411653 23.07769335590701 18.813953008217368 7 24.852667165604274 38.24439340370074 21.308627319414693 15.594312111280292 8 22.419675346460554 31.24839350692921 21.91148158662159 24.420449559988644 9 27.43389506825983 21.54058169243077 23.355441430746602 27.670081808562802 10-11 22.11659655733051 26.667165604273656 28.33621512813234 22.88002271026349 12-13 31.75927120699889 24.09393790807505 26.973238019045652 17.17355286588041 14-15 26.76931017574647 25.295878603318794 31.5008387313221 16.433972489612636 16-17 17.5941955377448 29.056687137337246 34.583148808251856 18.7659685166661 18-19 16.535394461792563 32.20785052517484 34.81400810343493 16.442746909597666 20-21 18.948256729205916 29.98828356860822 31.101034865415883 19.962424836769983 22-23 17.868848228341378 28.67201734238303 29.21887016439134 24.240264264884257 24-25 19.16912179139113 29.306550279646792 32.03256455412389 19.49176337483819 26-27 22.804321805524946 25.05756334032025 34.318796385522894 17.819318468631913 28-29 18.054865932024054 26.46308291826886 37.3775323234148 18.10451882629229 30-31 17.3792355931766 31.976980051098092 34.10575756793724 16.53802678778807 32-33 17.36256419520504 30.921210869957932 32.100286459005396 19.615938475831634 34-35 19.29411436780156 27.205028000150723 32.57401625242411 20.92684137962361 36-37 21.975316034872243 27.098054439355586 34.37392882659277 16.552700699179404 38-39 23.629995560648766 24.502121596927555 35.11950114081003 16.748381701613653 40-41 18.583463712181317 27.478200364114443 37.65600941102141 16.282326512682836 42-43 17.225458051675083 31.325541878125424 33.53295453137711 17.916045538822384 44-45 17.450702982209563 32.18228244301996 30.507141312353898 19.859873262416585 46-47 17.808612296954898 29.471018184314445 28.731846525232534 23.98852299349812 48-49 20.13331158615393 29.257113598731504 29.70693955247608 20.902635262638483 50-51 22.538284858964104 30.321040542981752 30.41544297917366 16.725231618880485 52-53 18.34225399365145 30.576376164546183 33.004206560169294 18.077163281633073 54-55 15.888151952308446 30.142042375287105 34.251464553923974 19.718341118480474 56-57 19.2219371362121 32.4796753632524 29.342421675758622 18.955965824776882 58-59 18.433654719630027 29.974295978198036 30.36986951853993 21.222179783632008 60-61 18.608022950144445 27.696618745473998 32.52345777909454 21.17190052528702 62-63 23.92627808921346 26.605892405608838 30.72828465147104 18.739544853706665 64-65 21.677495478532318 28.20771292190042 32.83881070947815 17.27598089008911 66-67 17.881548445840167 29.07478208624259 34.076049281776285 18.96762018614096 68-69 16.932540606272067 32.968695401732276 31.632297554462134 18.466466437533516 70-71 17.597821879274303 32.41513329376242 28.566982373738675 21.4200624532246 72-73 17.99984515729438 30.051407778265244 27.64861028671708 24.300136777723296 74-75 18.691191787934812 30.91055112875637 29.555310558974153 20.842946524334664 76-77 21.20106760646885 27.63371678061286 31.482254244448416 19.68296136846987 78-79 18.334091402462345 27.76765870349504 34.08018648500574 19.818063409036874 80-81 17.234094497495906 32.79555823264567 31.336354866531746 18.63399240332668 82-83 17.3404258613695 31.961177596392766 29.00128933158119 21.697107210656547 84-85 18.521974760638983 28.72430256264678 29.483960876409714 23.269761800304526 86-87 20.631289932903936 28.526263360052578 31.070653920516015 19.771792786527467 88-89 22.040310421217125 25.852412663602784 31.954552494034445 20.152724421145646 90-91 18.285803701471874 27.912594320485763 34.23568735365849 19.565914624383872 92-93 16.863876769535928 31.625394914843945 31.580387139131318 19.930341176488806 94-95 16.41179452081662 32.68304289883259 29.43210687081213 21.47305570953866 96-97 16.767563946199356 29.932194344212416 27.712316556461612 25.587925153126612 98-99 18.86887403545898 29.410513819711475 28.243619190172648 23.476992954656893 100-101 20.99397809569083 29.4200504070605 29.63621339023354 19.94975810701513 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8474.0 1 5450.5 2 7943.5 3 10365.0 4 5279.5 5 3584.0 6 5131.0 7 8360.5 8 10087.5 9 9102.0 10 6716.0 11 4544.5 12 3919.5 13 3926.0 14 4154.0 15 4038.5 16 3605.0 17 3528.0 18 3713.0 19 3882.0 20 4079.0 21 4327.0 22 4616.5 23 5049.0 24 5601.0 25 6396.0 26 7544.0 27 8683.5 28 9667.5 29 10632.5 30 11745.5 31 12888.5 32 14347.5 33 15882.0 34 17477.5 35 18672.0 36 19751.5 37 20842.5 38 20664.5 39 19928.5 40 19164.0 41 18636.0 42 18204.5 43 18595.0 44 21997.0 45 24763.0 46 29789.0 47 47837.0 48 117030.5 49 115097.5 50 51395.5 51 34704.5 52 18781.5 53 12507.0 54 10536.5 55 9256.5 56 8074.0 57 7015.5 58 6263.0 59 5687.5 60 5198.0 61 4588.0 62 3672.0 63 2467.5 64 1459.0 65 1093.0 66 856.0 67 640.0 68 538.5 69 475.5 70 390.0 71 311.0 72 265.0 73 280.0 74 239.5 75 175.0 76 130.0 77 84.5 78 49.0 79 31.0 80 25.5 81 19.5 82 15.5 83 10.0 84 4.0 85 3.0 86 4.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23814808124080622 2 0.0 3 0.0 4 0.0 5 0.028800743244986965 6 0.004645281168546285 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0022710263490670728 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.09685411236419E-4 26-27 9.806704689153268E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007174378693643707 36-37 0.016619783736354487 38-39 0.011871274097396061 40-41 0.007587292575292265 42-43 0.005884022813491961 44-45 0.00314846834757026 46-47 5.677565872667682E-4 48-49 0.0017548839970063744 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.001187127409739606 58-59 5.161423520606983E-4 60-61 0.0017032697618003046 62-63 3.612996464424889E-4 64-65 0.0013419701153578156 66-67 0.005213037755813053 68-69 1.548427056182095E-4 70-71 0.0 72-73 0.0 74-75 4.129138816485586E-4 76-77 0.059666055898216726 78-79 0.13987457740844925 80-81 0.001187127409739606 82-83 6.19370822472838E-4 84-85 0.0 86-87 0.1040542981754368 88-89 0.3500993574027717 90-91 0.385093808872487 92-93 3.612996464424889E-4 94-95 4.129138816485586E-4 96-97 5.161423520606983E-5 98-99 0.0 100-101 0.001187127409739606 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 968725.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.52080963143218 #Duplication Level Percentage of deduplicated Percentage of total 1 83.82944591090114 35.644959110858686 2 10.128456502620129 8.613403416163038 3 2.614563312188652 3.3352004660070134 4 1.0186795726242963 1.732603207319456 5 0.5485696843863038 1.1662813559682432 6 0.32556815017584 0.830605280141065 7 0.2338392354611698 0.6960123530782834 8 0.1586618691493447 0.5397144907093191 9 0.13566815428910184 0.5191847785417202 >10 0.8510948817554915 6.929441492080447 >50 0.0771182515278258 2.258564872546571 >100 0.05425027300429293 4.738696366300499 >500 0.009244441139639048 2.8025649563727018 >1k 0.010947364507467293 10.305552590838474 >5k 0.002432747668326065 7.748559893220018 >10k+ 0.0014596486009956391 12.13865536985448 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 33042 3.4108751193579185 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 30851 3.184701540684921 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 17845 1.8421120545046323 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14071 1.4525278071692171 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11001 1.1356164030039484 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10537 1.0877183927327156 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9765 1.0080260135745438 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9405 0.9708637642261736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9354 0.9655991122351545 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7960 0.8216986244806318 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7481 0.7722521871532169 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 6943 0.7167152700714857 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6846 0.7067021084415082 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6415 0.662210637693876 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5529 0.5707502129087203 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5209 0.5377171023768356 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4799 0.49539342950785825 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4775 0.4929159462179669 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4407 0.45492786910629956 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4284 0.44223076724560634 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4262 0.4399597408965393 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3984 0.41126222612196445 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3371 0.3479831737593228 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3324 0.34313143564995224 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3201 0.33043433378925907 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3048 0.3146403778162017 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2878 0.29709153784613795 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2841 0.2932720844408888 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2463 0.2542517226251 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2380 0.24568375958089242 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2190 0.22607035020258587 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2165 0.2234896384422824 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2136 0.22049601280033035 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 2085 0.2152313608093112 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1974 0.2037730005935637 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1935 0.19974709024749024 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1921 0.19830189166172033 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1851 0.1910758987328705 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1850 0.19097267026245837 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1802 0.1860177036826757 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1717 0.1772432836976438 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1704 0.175901313582286 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1664 0.1717721747658004 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1586 0.1637203540736535 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1574 0.16248161242870784 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1571 0.1621719270174714 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1540 0.1589718444346951 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1539 0.15886861596428295 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1472 0.15195230844666957 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1438 0.14844254045265684 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1417 0.14627474257400191 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1410 0.14555214328111693 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1397 0.14421017316575913 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1359 0.1402874912900978 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1295 0.13368086918372088 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1291 0.13326795530207233 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1206 0.12449353531704044 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1187 0.12253219437920977 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1167 0.12046762497096698 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1155 0.11922888332602131 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1011 0.1043639835866732 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 983 0.10147358641513328 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.1614235206069835E-5 0.0 12-13 0.0 1.0322847041213967E-4 0.0 1.0322847041213967E-4 0.0 14-15 0.0 1.0322847041213967E-4 0.0 1.0322847041213967E-4 0.0 16-17 0.0 1.0322847041213967E-4 0.0 2.5807117603034917E-4 0.0 18-19 0.0 1.0322847041213967E-4 0.0 4.645281168546285E-4 0.0 20-21 0.0 1.0322847041213967E-4 0.0 0.0010838989393274665 0.0 22-23 0.0 1.0322847041213967E-4 0.0 0.003974296110867377 0.0 24-25 0.0 1.0322847041213967E-4 0.0 0.006709850576789078 0.0 26-27 0.0 1.0322847041213967E-4 0.0 0.009290562337092571 0.0 28-29 0.0 1.0322847041213967E-4 0.0 0.015277813620996672 0.0 30-31 0.0 1.548427056182095E-4 0.0 0.03184598312214509 0.0 32-33 0.0 2.0645694082427934E-4 0.0 0.06007896977986528 0.0 34-35 0.0 2.0645694082427934E-4 0.0 0.1163384861544814 0.0 36-37 0.0 2.0645694082427934E-4 0.0 0.1978373635448657 0.0 38-39 0.0 2.0645694082427934E-4 0.0 0.29156881467908846 0.0 40-41 0.0 2.0645694082427934E-4 0.0 0.3972747683811195 0.0 42-43 0.0 2.0645694082427934E-4 0.0 0.49699347079924644 0.0 44-45 0.0 2.0645694082427934E-4 0.0 0.611215773310279 0.0 46-47 0.0 2.0645694082427934E-4 0.0 0.7563550027097474 0.0 48-49 0.0 2.0645694082427934E-4 0.0 0.9262174507729232 0.0 50-51 0.0 2.0645694082427934E-4 0.0 1.0922088311956437 0.0 52-53 0.0 2.0645694082427934E-4 0.0 1.2523678030400784 0.0 54-55 0.0 2.0645694082427934E-4 0.0 1.4319853415572015 0.0 56-57 0.0 2.0645694082427934E-4 0.0 1.6375648403829777 0.0 58-59 0.0 2.0645694082427934E-4 0.0 1.930630467883042 0.0 60-61 0.0 2.0645694082427934E-4 0.0 2.333376345196005 0.0 62-63 0.0 2.0645694082427934E-4 0.0 2.6762497096699267 0.0 64-65 0.0 2.0645694082427934E-4 0.0 2.9595086324808384 0.0 66-67 0.0 2.0645694082427934E-4 0.0 3.270794085008645 0.0 68-69 0.0 2.0645694082427934E-4 0.0 3.597460579627861 0.0 70-71 0.0 2.0645694082427934E-4 0.0 3.9887480967250766 0.0 72-73 0.0 2.0645694082427934E-4 0.0 4.4201398745774085 0.0 74-75 0.0 2.0645694082427934E-4 0.0 4.816124287078376 0.0 76-77 0.0 2.0645694082427934E-4 0.0 5.194353402668456 0.0 78-79 0.0 2.0645694082427934E-4 0.0 5.622596712173218 0.0 80-81 0.0 2.0645694082427934E-4 0.0 6.099357402771684 0.0 82-83 0.0 2.0645694082427934E-4 0.0 6.598570285684792 0.0 84-85 0.0 2.0645694082427934E-4 0.0 7.318124338692611 0.0 86-87 0.0 2.0645694082427934E-4 0.0 8.101628429120751 0.0 88-89 0.0 2.0645694082427934E-4 0.0 8.687295156004026 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 95 0.0 74.94737 3 TACCTGG 1610 0.0 58.375153 2 TATAACG 125 0.0 56.96 2 ACCTGGG 1595 0.0 56.24577 3 TAACGCA 145 0.0 49.103447 4 TATCACG 70 7.963172E-8 47.466667 2 CCTGGGA 1215 0.0 42.583267 4 AGTACTC 755 0.0 40.86534 5 CCTGGGG 665 0.0 36.403008 4 AAGTACT 810 0.0 35.746502 4 ATCACGC 95 8.7459375E-7 34.97544 3 TCACGCA 100 1.306058E-6 33.226665 4 AAAGTAC 860 0.0 33.11628 3 GTACCTG 2875 0.0 33.086926 1 GTAAGCG 60 1.5212026E-7 31.63219 94-95 AGCGTGG 60 0.0039732745 31.629742 8 CATTCCG 110 2.7578317E-6 30.192028 9 GAGCGTG 65 0.005877033 29.196688 7 GTACGTG 85 6.3574023E-4 27.977913 1 GTGTAGC 335 0.0 25.555944 1 >>END_MODULE