##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938699_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 993499 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.287881517746875 33.0 31.0 34.0 31.0 34.0 2 32.312127138527565 33.0 31.0 34.0 31.0 34.0 3 32.30335108540623 34.0 31.0 34.0 31.0 34.0 4 35.866054218474304 37.0 35.0 37.0 35.0 37.0 5 35.92670450599346 37.0 35.0 37.0 35.0 37.0 6 36.08212086776132 37.0 37.0 37.0 35.0 37.0 7 36.055154559793216 37.0 37.0 37.0 35.0 37.0 8 36.04403929948596 37.0 37.0 37.0 35.0 37.0 9 37.680602597486256 39.0 38.0 39.0 35.0 39.0 10-11 37.626159160703736 39.0 38.0 39.0 35.0 39.0 12-13 37.52538955751339 39.0 37.5 39.0 35.0 39.0 14-15 38.662503938101594 40.0 38.0 41.0 35.0 41.0 16-17 38.65683558815862 40.0 38.0 41.0 35.5 41.0 18-19 38.68847678759616 40.0 38.0 41.0 35.0 41.0 20-21 38.62766092366475 40.0 38.0 41.0 35.0 41.0 22-23 38.504548570255224 40.0 38.0 41.0 35.0 41.0 24-25 38.4655122954326 40.0 38.0 41.0 35.0 41.0 26-27 38.307070263784865 40.0 38.0 41.0 35.0 41.0 28-29 38.13533934105621 40.0 37.0 41.0 35.0 41.0 30-31 37.7793706888482 39.5 36.5 41.0 34.0 41.0 32-33 37.37611764078273 39.0 35.0 41.0 33.5 41.0 34-35 37.008312539821375 39.0 35.0 41.0 33.0 41.0 36-37 36.70616175758607 39.0 35.0 41.0 33.0 41.0 38-39 36.49965978828364 38.0 35.0 41.0 33.0 41.0 40-41 36.290882527310046 38.0 35.0 40.5 32.5 41.0 42-43 35.97994009052853 38.0 35.0 40.0 31.0 41.0 44-45 35.647419373346125 38.0 35.0 41.0 30.5 41.0 46-47 35.22004450935532 38.0 35.0 41.0 28.0 41.0 48-49 34.860111585416796 38.0 35.0 41.0 25.0 41.0 50-51 33.69548132408789 37.0 34.0 40.0 23.0 40.5 52-53 33.87683278996758 37.5 34.0 39.5 23.0 40.5 54-55 34.38232801442176 38.0 35.0 40.0 23.0 41.0 56-57 34.29411604843085 38.0 35.0 40.0 23.0 41.0 58-59 34.11645306135185 37.5 35.0 40.0 22.5 41.0 60-61 33.8355146809408 37.0 35.0 40.0 22.0 41.0 62-63 33.515886276684725 36.0 34.5 40.0 20.0 41.0 64-65 33.151499900855455 36.0 34.0 39.5 17.5 41.0 66-67 32.76142401753802 35.0 33.5 39.0 11.5 41.0 68-69 32.40172008225474 35.0 33.0 39.0 7.5 40.5 70-71 31.992707088784186 35.0 33.0 38.5 2.0 40.0 72-73 31.583107280430077 35.0 33.0 37.0 2.0 40.0 74-75 31.17643097778659 35.0 32.5 37.0 2.0 39.0 76-77 30.75302944441816 35.0 32.0 36.0 2.0 39.0 78-79 30.347961095079107 35.0 31.5 36.0 2.0 38.5 80-81 29.92637788261488 35.0 31.0 35.5 2.0 37.0 82-83 29.487681920162977 35.0 30.5 35.0 2.0 37.0 84-85 29.08972933037678 34.5 30.0 35.0 2.0 36.5 86-87 28.762939368836808 34.0 30.0 35.0 2.0 36.0 88-89 28.411240474323577 34.0 29.0 35.0 2.0 36.0 90-91 27.99840563503335 34.0 29.0 35.0 2.0 35.0 92-93 27.59828746682181 34.0 28.0 35.0 2.0 35.0 94-95 27.152430450357777 34.0 27.0 35.0 2.0 35.0 96-97 26.629066058445957 34.0 25.0 35.0 2.0 35.0 98-99 26.073682509997496 34.0 24.5 35.0 2.0 35.0 100-101 24.05079270336457 31.0 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1382078086215941 1101 2 0.10245505447174708 1101 3 0.1429041752664375 1101 4 0.10888200526427028 1101 5 0.05109533809528699 1101 6 0.054698354172806773 1101 7 0.03354141917590425 1101 8 0.0719555938924259 1101 9 0.13697177528018756 1101 10-11 0.28376564711927443 1101 12-13 0.2119584298414665 1101 14-15 0.2752015403969139 1101 16-17 0.27705513396215764 1101 18-19 0.22147863169296755 1101 20-21 0.27326112728005114 1101 22-23 0.29974340480176664 1101 24-25 0.21791726343774798 1101 26-27 0.22977977896410096 1101 28-29 0.2424477861421792 1101 30-31 0.17505564722778644 1101 32-33 0.21113462788635928 1101 34-35 0.2201685933532005 1101 36-37 0.22465161030394398 1101 38-39 0.27434455825130755 1101 40-41 0.2578980789538079 1101 42-43 0.29145650268817747 1101 44-45 0.42607284971141013 1101 46-47 0.546638731940746 1101 48-49 0.3957249166458894 1101 50-51 0.38320115820331324 1101 52-53 0.5489845505811957 1101 54-55 0.5571257776877516 1101 56-57 0.416041383192443 1101 58-59 0.3548046596167538 1101 60-61 0.3496446290381314 1101 62-63 0.3753502113329148 1101 64-65 0.42097188517698214 1101 66-67 0.4354255258111408 1101 68-69 0.467977951374003 1101 70-71 0.31502954882503786 1101 72-73 0.1456895651788379 1101 74-75 0.04810153479456858 1101 76-77 0.12056794340561439 1101 78-79 0.2486180906730162 1101 80-81 0.23833946749131485 1101 82-83 0.33428196518748976 1101 84-85 0.24244947248931226 1101 86-87 0.2031467369256852 1101 88-89 0.08811371093224807 1101 90-91 0.25196284241751954 1101 92-93 0.32485412699089977 1101 94-95 0.1827893262632756 1101 96-97 0.1488084129083127 1101 98-99 0.12624605179630422 1101 100-101 0.4777845045942648 1102 1 0.2363294054563525 1102 2 0.16941782252352766 1102 3 0.22106245666198276 1102 4 0.12882515379832427 1102 5 0.1464108541279714 1102 6 0.1963545372498885 1102 7 0.055974700331368865 1102 8 0.07624442238729756 1102 9 0.23759502868215066 1102 10-11 0.18045395319527557 1102 12-13 0.11555053977991037 1102 14-15 0.012912692695458361 1102 16-17 0.16928895482242012 1102 18-19 0.2480067549223577 1102 20-21 0.1578252820466446 1102 22-23 0.08964458696654987 1102 24-25 -0.010999197558497542 1102 26-27 0.06948985569437127 1102 28-29 -0.026318959591563384 1102 30-31 0.021612868420326947 1102 32-33 -0.023231218979610446 1102 34-35 -0.20323750089347214 1102 36-37 -0.16771905741267545 1102 38-39 0.08301632746508147 1102 40-41 0.10988451911091346 1102 42-43 -0.012444352964806171 1102 44-45 0.008711545200746684 1102 46-47 0.06819043124258428 1102 48-49 -0.05574774759434575 1102 50-51 -0.026582191615666773 1102 52-53 -0.07285572753002612 1102 54-55 -0.06324855848495048 1102 56-57 -0.09103191870612903 1102 58-59 -0.160330122532649 1102 60-61 -0.12710869129875135 1102 62-63 -0.1461030542652253 1102 64-65 -0.19504207800401474 1102 66-67 -0.2904178829720294 1102 68-69 -0.3893047022065801 1102 70-71 -0.5234532276776349 1102 72-73 -0.3885307918714176 1102 74-75 -0.43366182097844685 1102 76-77 -0.2635703055308234 1102 78-79 -0.5649585579714795 1102 80-81 -0.6709651584125957 1102 82-83 -0.6682403384492765 1102 84-85 -0.5814833621132536 1102 86-87 -0.5877834375660598 1102 88-89 -0.7224763150926634 1102 90-91 -0.5385615931492183 1102 92-93 -0.6360984231622737 1102 94-95 -0.5938698818495851 1102 96-97 -0.598906004508212 1102 98-99 -0.7432078092057033 1102 100-101 -0.3795629711814392 1103 1 -0.09960971089943627 1103 2 -4.0286341397433034E-4 1103 3 -0.07077701672751857 1103 4 -0.016048726556469717 1103 5 -0.009418893366998304 1103 6 0.05989685836765091 1103 7 -0.06828764827096023 1103 8 -0.08506325867776354 1103 9 0.011862786886240428 1103 10-11 -0.028235670429310744 1103 12-13 0.08740068052446048 1103 14-15 0.19878566006131848 1103 16-17 0.19662073044352724 1103 18-19 0.10607493868178608 1103 20-21 0.12319718742227082 1103 22-23 0.10084103902703845 1103 24-25 0.026338917736254075 1103 26-27 -0.02309073313602994 1103 28-29 0.030972519241700525 1103 30-31 0.03655997769997299 1103 32-33 0.07589214081964002 1103 34-35 0.08281512219394926 1103 36-37 0.12517766095206184 1103 38-39 0.0861928076555003 1103 40-41 0.19123496450145439 1103 42-43 0.1468119501703029 1103 44-45 0.22182375552606004 1103 46-47 0.37119551247202764 1103 48-49 0.3642916950458428 1103 50-51 0.38775154474881646 1103 52-53 0.1852762325869719 1103 54-55 0.26897596466339024 1103 56-57 0.15602317002083055 1103 58-59 0.025979409185644897 1103 60-61 -0.046236094063530686 1103 62-63 -0.018901014181025744 1103 64-65 0.033294839585927605 1103 66-67 0.18711993204237132 1103 68-69 0.15508535095936082 1103 70-71 0.10711747581734699 1103 72-73 -0.04659793167261128 1103 74-75 0.0011638664680511113 1103 76-77 -0.01993892528517094 1103 78-79 0.022284557123967375 1103 80-81 -0.0248660507121663 1103 82-83 0.27845278148634023 1103 84-85 0.30627404477041864 1103 86-87 0.15257418456281968 1103 88-89 -0.03676373767367025 1103 90-91 -0.07109038267015322 1103 92-93 -0.05311181050304725 1103 94-95 -0.07565945403550245 1103 96-97 -0.10134174887890524 1103 98-99 -0.08882631649727912 1103 100-101 -0.08046276827392163 1104 1 0.14315830367110038 1104 2 0.11730653962026594 1104 3 0.2191417990288116 1104 4 0.1762087379375359 1104 5 0.17584781334281274 1104 6 0.10222310664805434 1104 7 0.06819488452243405 1104 8 0.05116351468450375 1104 9 0.1597440525079108 1104 10-11 0.14168643919848734 1104 12-13 0.042651499148398386 1104 14-15 0.16480550079294431 1104 16-17 0.17408483693245813 1104 18-19 0.18484496832662956 1104 20-21 0.16633043421074944 1104 22-23 0.19281271173246495 1104 24-25 0.08177864957636416 1104 26-27 0.042156016587867384 1104 28-29 0.06868540990454619 1104 30-31 0.11416455811887971 1104 32-33 0.11658017244081265 1104 34-35 0.2612577022640963 1104 36-37 0.2998991350564211 1104 38-39 0.30553267706319076 1104 40-41 0.20096738588449625 1104 42-43 0.36818917595550005 1104 44-45 0.6666669091173532 1104 46-47 0.45406447451500753 1104 48-49 0.3180021443686627 1104 50-51 0.6084486829557889 1104 52-53 0.5039350456307048 1104 54-55 0.4922742925392356 1104 56-57 0.3843582148756113 1104 58-59 0.3874779269434967 1104 60-61 0.31400106468169753 1104 62-63 0.49614229054083836 1104 64-65 0.2962194099294564 1104 66-67 0.30423740699925617 1104 68-69 0.3640175553343994 1104 70-71 0.32542558842899894 1104 72-73 0.15856085230754857 1104 74-75 0.0020619308341736087 1104 76-77 0.0789837849897701 1104 78-79 0.13921215007895782 1104 80-81 0.15319095263982874 1104 82-83 0.16596513350432218 1104 84-85 0.30977620516257787 1104 86-87 0.39671109336132915 1104 88-89 0.2866285624173983 1104 90-91 0.18562620875415448 1104 92-93 0.3441610576839693 1104 94-95 0.2550665539860475 1104 96-97 0.3017787099380165 1104 98-99 0.4915925864497659 1104 100-101 0.6465963857823844 1105 1 0.18454720351726195 1105 2 0.1299056481366918 1105 3 0.126660240115946 1105 4 0.14358280602163376 1105 5 0.11874750171801907 1105 6 0.14219194416925518 1105 7 0.17849625330472918 1105 8 0.19221120511091527 1105 9 0.25443262495080887 1105 10-11 0.24037189539350834 1105 12-13 0.1914395154002264 1105 14-15 0.33600683407369303 1105 16-17 0.26669854053389486 1105 18-19 0.25133051130324446 1105 20-21 0.2786721564307797 1105 22-23 0.27722259694409246 1105 24-25 0.20013492743522932 1105 26-27 0.36246586629793853 1105 28-29 0.24624645286143476 1105 30-31 0.22363262643965243 1105 32-33 0.28295625227987387 1105 34-35 0.3032737963037775 1105 36-37 0.3059077737689009 1105 38-39 0.4697086464529505 1105 40-41 0.4160802019469827 1105 42-43 0.4539698157724672 1105 44-45 0.4050283149790488 1105 46-47 0.5552557490209438 1105 48-49 0.7005679208666322 1105 50-51 0.7377078837762738 1105 52-53 0.6835512343412091 1105 54-55 0.8975088000537355 1105 56-57 0.8202936809321741 1105 58-59 0.8438780689497278 1105 60-61 0.9599982640032252 1105 62-63 0.9012091518086365 1105 64-65 0.8692816165485269 1105 66-67 1.0418838509265171 1105 68-69 0.9763326513858388 1105 70-71 0.9161182632741713 1105 72-73 1.0087462493333028 1105 74-75 0.9913138341121517 1105 76-77 0.8604075947014174 1105 78-79 0.8369213134255702 1105 80-81 0.8787757421944811 1105 82-83 1.062398213067258 1105 84-85 0.9153933405813035 1105 86-87 0.9091652371820871 1105 88-89 0.823733607583268 1105 90-91 1.0030387368595548 1105 92-93 0.8608507346197882 1105 94-95 0.9398284963157373 1105 96-97 0.8030183060091751 1105 98-99 0.8243555938262013 1105 100-101 1.1732669313229955 1106 1 0.0011458881438315416 1106 2 -0.012769682287640194 1106 3 0.01780202219398319 1106 4 0.14355118646810183 1106 5 0.012379323636693584 1106 6 -0.019427451264881768 1106 7 -0.0168770155247131 1106 8 0.03747696745835327 1106 9 0.05030864468141516 1106 10-11 0.019193118437833334 1106 12-13 0.021448843168521137 1106 14-15 -0.028519188818869168 1106 16-17 0.06015764064139972 1106 18-19 0.10672045944716757 1106 20-21 0.009184630344648781 1106 22-23 0.06128080854214346 1106 24-25 0.08287974182844948 1106 26-27 -0.0722318507702937 1106 28-29 -0.040517206785693816 1106 30-31 -0.17224220755768727 1106 32-33 -0.06743385396182333 1106 34-35 -0.014971081328546632 1106 36-37 0.05338216193053569 1106 38-39 -0.09911921669762336 1106 40-41 -0.09765256539634493 1106 42-43 -0.03190217246505256 1106 44-45 -0.03560992570898236 1106 46-47 0.07198644528351394 1106 48-49 0.18939633735938344 1106 50-51 0.11002812661444494 1106 52-53 0.09250470616818518 1106 54-55 0.043648447811904134 1106 56-57 0.22204975188645193 1106 58-59 -0.010264883051796403 1106 60-61 -0.09238743131075466 1106 62-63 -0.2557799552551714 1106 64-65 -0.10660649766129637 1106 66-67 -0.1380254800037264 1106 68-69 0.17362878431443818 1106 70-71 0.02799021989214623 1106 72-73 0.08716076587033328 1106 74-75 -0.0024986145060772458 1106 76-77 -0.18422735755305197 1106 78-79 0.06877564214245169 1106 80-81 -0.06242176934665267 1106 82-83 0.039317915211061205 1106 84-85 0.06111205303308509 1106 86-87 0.12224150354520447 1106 88-89 0.26620973482035026 1106 90-91 0.3323465057838568 1106 92-93 0.42939797423276715 1106 94-95 0.5907150295478871 1106 96-97 0.7277855070462955 1106 98-99 0.8653009394279216 1106 100-101 1.0071377970663669 1107 1 0.07341572941367502 1107 2 -0.031277618795577666 1107 3 0.056389323781289136 1107 4 0.11661467853159735 1107 5 -0.019033374776000755 1107 6 -0.03989570523313546 1107 7 -0.08556749171518874 1107 8 -0.03181668333530041 1107 9 -0.14381040293763903 1107 10-11 0.04182010256482016 1107 12-13 0.07179011300978999 1107 14-15 -0.0030509348506129186 1107 16-17 0.08939176762552137 1107 18-19 -0.08740255642584316 1107 20-21 -0.006184417274397447 1107 22-23 0.06189191965325591 1107 24-25 -0.017171845473136216 1107 26-27 -0.11085883489728587 1107 28-29 -0.13119577821426276 1107 30-31 -0.1508255408910273 1107 32-33 0.06264947937150822 1107 34-35 0.15806463295716355 1107 36-37 -0.06054640949803769 1107 38-39 0.18958713250872705 1107 40-41 -0.05657716857094641 1107 42-43 0.03272877991589951 1107 44-45 0.06719166159260226 1107 46-47 0.18889120718827712 1107 48-49 0.11732887704192763 1107 50-51 0.40890907899539286 1107 52-53 0.22032613473961504 1107 54-55 0.1762000351134887 1107 56-57 0.12532356141026213 1107 58-59 0.20185416456725847 1107 60-61 0.14780304487972273 1107 62-63 0.16745417172895571 1107 64-65 0.1689768356720336 1107 66-67 0.13323245650421 1107 68-69 0.21695814939380398 1107 70-71 0.3403513310032622 1107 72-73 0.22920441666398617 1107 74-75 0.3040767823193242 1107 76-77 0.2608996265739272 1107 78-79 0.40271215007895833 1107 80-81 0.39250283382795104 1107 82-83 0.2776631533063032 1107 84-85 0.24550887842990932 1107 86-87 0.41738436068806095 1107 88-89 0.3527176713282856 1107 90-91 0.3340965057838581 1107 92-93 0.2747551170899101 1107 94-95 0.33723487081772774 1107 96-97 0.3608529673637584 1107 98-99 0.4200826854596684 1107 100-101 0.34822509865367124 1108 1 -0.005736979945929477 1108 2 -0.032652003032026755 1108 3 0.016556811466017507 1108 4 -0.021035567773822095 1108 5 0.09844938384468804 1108 6 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2316 48-49 -1.2961486390180283 2316 50-51 -1.7957279659724854 2316 52-53 -1.5928826447036357 2316 54-55 -1.2509423631734435 2316 56-57 -1.0702064171764576 2316 58-59 -1.2438289189445229 2316 60-61 -0.9475674047990807 2316 62-63 -1.0398638154230824 2316 64-65 -1.0762041065121224 2316 66-67 -1.2203210766863677 2316 68-69 -1.3352881032828563 2316 70-71 -1.0819238295963132 2316 72-73 -1.16468209297199 2316 74-75 -1.1373247166100136 2316 76-77 -1.0249130072590482 2316 78-79 -0.920209691462798 2316 80-81 -0.825748772167767 2316 82-83 -0.5102608295630766 2316 84-85 -0.6557823421268374 2316 86-87 -0.6801445472348497 2316 88-89 -0.7691774036181798 2316 90-91 -1.0981272629527616 2316 92-93 -1.1880500220963839 2316 94-95 -1.2988100970623577 2316 96-97 -1.2793087028717878 2316 98-99 -1.4569890490157071 2316 100-101 -1.6417567000615314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2983.0 3 601.0 4 585.0 5 555.0 6 580.0 7 712.0 8 850.0 9 1101.0 10 1371.0 11 2137.0 12 3521.0 13 5737.0 14 6407.0 15 7052.0 16 10463.0 17 15403.0 18 12316.0 19 6715.0 20 6055.0 21 6001.0 22 6175.0 23 6765.0 24 7324.0 25 8151.0 26 9340.0 27 10785.0 28 12870.0 29 15563.0 30 18711.0 31 23542.0 32 30906.0 33 42812.0 34 67679.0 35 143715.0 36 134265.0 37 168182.0 38 164800.0 39 30758.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.81284394792754 20.377541435016266 15.104681118695042 16.704933498361143 2 14.402278205842386 24.557499614691487 40.65483307830778 20.385389101158342 3 20.294509005601494 32.93831105058468 29.81573796978357 16.951441974030264 4 10.688276402599534 19.14542950659807 38.833755272561326 31.332538818241073 5 10.297481223538137 42.327999145562664 32.49783871689307 14.876680914006121 6 24.189909091000622 38.606471464199736 19.927184197198727 17.276435247600915 7 23.244520608633316 39.2702803934046 21.724346943542734 15.760852054419358 8 25.22416298222102 32.21251003132492 21.293293285229964 21.270033701224097 9 27.701359024265486 19.06923401457399 22.78714258527759 30.44226437588293 10-11 22.97462959858349 29.81340599136783 26.12260741250904 21.089356997539635 12-13 27.136362492133415 28.921686595342983 27.381397105097545 16.560553807426054 14-15 23.347681835802785 28.166060001913134 30.92897743005734 17.557280732226737 16-17 17.012803679597887 32.518990043820075 30.90020460110839 19.568001675473642 18-19 16.441702471300072 30.356270786741995 36.548164825836935 16.653861916120995 20-21 18.944077801295748 28.11299836672057 35.81936855927032 17.123555272713357 22-23 17.508177273624465 29.04880785018268 35.75200705743024 17.691007818762614 24-25 17.66948928487797 29.23315934321879 36.345754364231055 16.751597007672185 26-27 17.552314751165948 28.54947900535876 37.553865825058004 16.344340418417293 28-29 16.216431247095127 30.0444587229787 37.43939106303632 16.29971896688986 30-31 16.314996326959513 30.395656195354398 36.626733994833074 16.66261348285302 32-33 16.07695866849863 30.588472814290945 36.3974284680011 16.937140049209322 34-35 17.021491292686424 30.081452584545403 36.120921568587875 16.776134554180295 36-37 17.143760825058962 29.718412048480097 36.793882793284574 16.34394433317637 38-39 17.232039256236963 30.227686376451107 36.41426160418547 16.126012763126457 40-41 16.280752861462442 31.03975264883822 36.56969958369086 16.109794906008478 42-43 15.899576305649981 31.548886507807804 35.20113483604135 17.35040235050086 44-45 16.345138068261907 31.15158686172763 34.68416604061845 17.819109029392017 46-47 16.877290515409545 31.567104081222297 34.137988175964125 17.417617227404037 48-49 16.917292473242156 31.525694703407602 33.955956556846076 17.601056266504163 50-51 16.66550461737759 31.978232290621456 34.4739099725049 16.882353119496056 52-53 16.527909219938426 32.70035616965782 33.41810194769071 17.353632662713046 54-55 15.759103712217856 31.89086913137386 34.11077288401056 18.23925427239772 56-57 17.080332603072147 32.138749288386826 32.72294139843417 18.057976710106853 58-59 17.459959919515804 31.581007426202618 33.08488416255222 17.87414849172936 60-61 16.952498669550735 31.597134914119124 34.322763456724296 17.127602959605845 62-63 17.350051754631014 31.695859579001713 33.85247934740164 17.101609318965636 64-65 16.763616866555388 32.68998019557814 33.71254328900963 16.833859648856837 66-67 16.79301396549938 32.06799052963783 33.0877474031044 18.051248101758386 68-69 16.433095845658787 32.537883554724004 33.290677534798085 17.738343064819126 70-71 17.096035187076644 32.357616007835524 32.58534346870466 17.961005336383177 72-73 16.868339307290483 31.62228689447042 33.24493258010771 18.264441218131385 74-75 16.391975587806233 33.03604679306987 32.804415943115025 17.767561676008874 76-77 16.668359142324068 31.80752863694073 33.201774927008834 18.32233729372637 78-79 16.544289047232525 31.575951320359973 33.76202961812829 18.117730014279214 80-81 16.75082006629227 32.40082678442081 32.89483653732501 17.953516611961906 82-83 16.42972865246672 31.87203174957603 33.11992830480159 18.578311293155664 84-85 16.486707397038273 31.723163470777287 32.80110046768866 18.989028664495773 86-87 16.68312004567202 31.945809945789737 32.89936493661997 18.471705071918276 88-89 16.9636780261785 31.20021423825416 32.66739258402156 19.168715151545772 90-91 16.642741106235047 30.989588465201827 33.081546495675966 19.28612393288716 92-93 16.95815035070512 31.749766171318733 32.98235029279747 18.309733185178672 94-95 16.65449675960433 31.524590371034776 32.78517376858648 19.03573910077441 96-97 16.140592903651264 31.060106473024224 33.11103868048239 19.68826194284213 98-99 16.902364252187574 31.135982019374246 32.231322690686646 19.730331037751533 100-101 17.35939084396566 30.27050978066078 33.03744878775585 19.332650587617714 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23427.0 1 14343.5 2 9015.0 3 11085.0 4 7259.0 5 6015.5 6 9951.0 7 18261.5 8 25028.0 9 23454.5 10 15371.5 11 9098.5 12 7620.0 13 7137.5 14 6487.0 15 5622.5 16 4948.5 17 4620.5 18 4395.5 19 4184.0 20 4151.0 21 4448.5 22 4791.5 23 5187.0 24 5673.5 25 6286.0 26 7239.5 27 8408.5 28 9540.0 29 10830.0 30 12197.5 31 13582.0 32 15928.5 33 18428.5 34 20385.0 35 21762.0 36 24025.0 37 26305.5 38 26123.0 39 25465.5 40 24718.0 41 23857.0 42 23338.5 43 23700.5 44 24783.5 45 25461.5 46 26028.5 47 32559.5 48 56700.5 49 55376.0 50 32800.0 51 24711.0 52 17962.0 53 15545.0 54 14331.0 55 13115.5 56 12045.5 57 10804.5 58 10109.0 59 9407.0 60 8542.5 61 7817.0 62 6675.5 63 4615.5 64 2667.0 65 1943.5 66 1534.0 67 1172.0 68 968.5 69 736.0 70 560.5 71 459.0 72 401.5 73 412.5 74 345.5 75 251.5 76 185.0 77 115.0 78 84.5 79 67.5 80 56.0 81 43.5 82 29.5 83 24.0 84 18.5 85 13.0 86 10.5 87 7.5 88 4.5 89 3.0 90 3.5 91 3.5 92 1.5 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13517879736164806 2 0.07931563091658873 3 0.07327636967928502 4 0.0865627444013532 5 0.1039759476355789 6 0.031001541018159055 7 0.03251135632748498 8 0.036235567423822265 9 0.036235567423822265 10-11 0.03558131412311437 12-13 0.03764472837919313 14-15 0.03663818483964251 16-17 0.034927060822406465 18-19 0.03804734579501338 20-21 0.04076501335180006 22-23 0.050629140039396114 24-25 0.06305995275284626 26-27 0.064267805000307 28-29 0.055913493622036854 30-31 0.04554609516466549 32-33 0.0397081426352719 34-35 0.25093130440996925 36-37 0.39225001736287607 38-39 0.37186751068697604 40-41 0.3512333681261884 42-43 0.3777054632163696 44-45 0.38505323105508915 46-47 0.38449963210833626 48-49 0.40020171132532595 50-51 0.38933104109817923 52-53 0.3968297904678314 54-55 0.38867678779747133 56-57 0.36945180619205453 58-59 0.35138434965712095 60-61 0.32531487198275993 62-63 0.37569237613726836 64-65 0.4100155108359445 66-67 0.4123305609769109 68-69 0.42118814412495637 70-71 0.4185711309221247 72-73 0.409210276004304 74-75 0.40196316251953956 76-77 0.3849525767011341 78-79 0.3622046927072901 80-81 0.3663818483964252 82-83 0.3778061175703247 84-85 0.37624597508402124 86-87 0.3859087930637072 88-89 0.39723240788365166 90-91 0.3997487667325282 92-93 0.4018625081655845 94-95 0.4062912997396072 96-97 0.40729784327915786 98-99 0.4015605451037193 100-101 0.3892807139212017 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 993499.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.61482946214877 #Duplication Level Percentage of deduplicated Percentage of total 1 74.48109839301965 30.250371093860156 2 13.382318826967929 10.870411939308104 3 4.787520817899229 5.833330246963925 4 2.323166169877955 3.774199912073059 5 1.3096104884539586 2.6594803325199434 6 0.809496881378836 1.9726546672405618 7 0.5337492331503417 1.5174693855967667 8 0.3683976772926913 1.1969927069995496 9 0.27201314504302093 0.9942990747646531 >10 1.5162108013747229 11.262521722114961 >50 0.12940484211807507 3.5828677381266076 >100 0.07139505512003423 5.261844324379338 >500 0.006693286415818169 2.0632156652091727 >1k 0.0061974874220538595 5.396660143236954 >5k 0.0014873969812929265 3.8523695970248566 >10k+ 0.0012394974844107721 9.511311450581442 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26809 2.6984425751812533 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22210 2.235533201341924 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18576 1.8697552790692291 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16194 1.6299966079482717 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10678 1.0747871915321505 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8226 0.8279827156343388 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7377 0.7425271691264913 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5785 0.5822854376300328 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5767 0.5804736592588418 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5589 0.5625571842548407 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5518 0.5554107251240313 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4690 0.47206892004924017 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4021 0.4047311572533037 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3987 0.4013089092188316 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3960 0.39859124166204496 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3643 0.3666838114582903 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2742 0.2759942385447796 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2369 0.23845016451954157 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2193 0.22073499822345063 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2088 0.21016629105816914 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2009 0.20221459709571926 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1921 0.19335701394767382 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1882 0.18943149414342642 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1822 0.1833922329061227 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1714 0.17252156267897603 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1706 0.17171632784733554 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1535 0.15450443332101993 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1502 0.15118283964050291 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1477 0.14866648079162637 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1395 0.1404128237673113 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1324 0.1332663646365019 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1283 0.12913953612434437 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1130 0.1137394199692199 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1083 0.10900866533333198 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1081 0.10880735662542186 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1043 0.1049824911751295 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 999 0.10055369960110679 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 998 0.10045304524715173 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.0065435395506186E-4 0.0 14-15 0.0 0.0 0.0 1.0065435395506186E-4 0.0 16-17 0.0 0.0 0.0 1.509815309325928E-4 0.0 18-19 0.0 0.0 0.0 2.0130870791012373E-4 0.0 20-21 0.0 0.0 0.0 0.0010568707165281494 0.0 22-23 0.0 0.0 0.0 0.0029189762646967938 0.0 24-25 0.0 0.0 0.0 0.004982390520775562 0.0 26-27 0.0 0.0 0.0 0.008253657024315072 0.0 28-29 0.0 0.0 0.0 0.014041282376731128 0.0 30-31 0.0 0.0 0.0 0.030598923602338804 0.0 32-33 0.0 0.0 0.0 0.051082084632193894 0.0 34-35 0.0 0.0 0.0 0.08117773646475739 0.0 36-37 0.0 0.0 0.0 0.126069578328715 0.0 38-39 0.0 0.0 0.0 0.18902887672760615 0.0 40-41 0.0 0.0 0.0 0.26446931501692506 0.0 42-43 0.0 0.0 0.0 0.33724241292643475 0.0 44-45 0.0 0.0 0.0 0.4240064660356981 0.0 46-47 0.0 0.0 0.0 0.5331661128999627 0.0 48-49 0.0 0.0 0.0 0.6552598442474526 0.0 50-51 0.0 0.0 0.0 0.7890294806537299 0.0 52-53 0.0 0.0 0.0 0.9204337397420632 0.0 54-55 0.0 0.0 0.0 1.055864172988599 0.0 56-57 0.0 0.0 0.0 1.2175653926174057 0.0 58-59 0.0 0.0 0.0 1.408406047716203 0.0 60-61 0.0 0.0 0.0 1.6390554998042273 0.0 62-63 0.0 0.0 0.0 1.8647728885484534 0.0 64-65 0.0 0.0 0.0 2.0745868893677804 0.0 66-67 0.0 0.0 0.0 2.3121814918787034 0.0 68-69 0.0 0.0 0.0 2.5903901262104947 0.0 70-71 0.0 0.0 0.0 2.911427188150164 0.0 72-73 0.0 0.0 0.0 3.238151221088295 0.0 74-75 0.0 0.0 0.0 3.5577287948956164 0.0 76-77 0.0 0.0 0.0 3.8772057143489826 0.0 78-79 0.0 0.0 0.0 4.265429557553657 0.0 80-81 0.0 0.0 0.0 4.703275997258175 0.0 82-83 0.0 0.0 0.0 5.135989064910986 0.0 84-85 0.0 0.0 0.0 5.574892375332033 0.0 86-87 0.0 0.0 0.0 6.036543569746925 0.0 88-89 0.0 0.0 0.0 6.511632120414816 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 60 1.5127534E-6 47.405216 2 TATAACG 70 2.0773627E-4 33.860867 2 TACCTGG 580 0.0 28.606596 2 ATAACGC 85 6.4818206E-4 27.88542 3 ATCACGC 105 6.9994305E-5 27.088694 3 GTCGGAT 45 8.8830775E-4 26.422754 66-67 ATGGGAG 2790 0.0 24.63712 5 ATGGGAT 3525 0.0 24.341402 5 CATGGGG 2065 0.0 24.333912 4 GTACATG 14525 0.0 24.23251 1 GTATATG 360 0.0 23.718142 1 GAGTACT 12575 0.0 23.716541 12-13 CACGCAG 120 1.7339096E-4 23.702608 5 TACATGG 14295 0.0 23.677736 2 ACATGGG 14375 0.0 22.820457 3 AGTACTT 12815 0.0 22.66238 12-13 ACGTATA 130 2.9953537E-4 21.863909 7 TATATGG 350 0.0 21.670956 2 CATGGGA 11435 0.0 21.30851 4 GTACTAG 135 3.829998E-4 21.08279 1 >>END_MODULE