##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938699_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 993499 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.531435864555476 31.0 31.0 34.0 30.0 34.0 2 31.696486861083905 31.0 31.0 34.0 30.0 34.0 3 31.700635833553935 31.0 31.0 34.0 30.0 34.0 4 35.488783582067015 37.0 35.0 37.0 33.0 37.0 5 35.431882669232685 37.0 35.0 37.0 33.0 37.0 6 35.546571259759695 37.0 35.0 37.0 33.0 37.0 7 35.47753646455608 37.0 35.0 37.0 33.0 37.0 8 35.48953043737336 37.0 35.0 37.0 33.0 37.0 9 36.98529741851778 39.0 37.0 39.0 33.0 39.0 10-11 36.88840602758533 39.0 37.0 39.0 32.0 39.0 12-13 36.78624940739749 39.0 37.0 39.0 32.0 39.0 14-15 37.62163877366761 39.0 36.5 41.0 32.0 41.0 16-17 37.57254561906957 39.0 36.5 41.0 32.0 41.0 18-19 37.71420957645654 39.5 36.5 41.0 32.0 41.0 20-21 37.813125126447034 40.0 37.0 41.0 33.0 41.0 22-23 37.67080037322634 40.0 36.0 41.0 32.0 41.0 24-25 37.49997735277036 39.0 36.0 41.0 32.0 41.0 26-27 37.17670677071642 39.0 35.5 41.0 31.5 41.0 28-29 36.88559726783822 39.0 35.0 40.5 31.0 41.0 30-31 36.41176941295362 38.0 35.0 40.0 30.5 41.0 32-33 35.89238841709957 38.0 35.0 40.0 30.0 41.0 34-35 35.55281182970491 38.0 35.0 40.0 30.0 41.0 36-37 35.27932338130184 37.5 34.5 40.0 28.0 41.0 38-39 35.05635838586652 37.0 34.0 40.0 27.0 41.0 40-41 34.78116837560984 37.0 34.0 40.0 25.5 41.0 42-43 34.44268942394507 37.0 33.0 40.0 24.5 41.0 44-45 33.94988671352462 37.0 33.0 40.0 23.5 41.0 46-47 33.333229323834246 36.0 33.0 40.0 20.5 41.0 48-49 32.82489866622916 36.0 33.0 40.0 17.5 41.0 50-51 32.53291296719976 36.0 32.5 40.0 15.0 41.0 52-53 32.30738229228213 35.0 32.0 39.5 12.5 41.0 54-55 32.079562737355545 35.0 31.0 39.0 9.0 41.0 56-57 31.81181259367146 35.0 31.0 39.0 8.0 40.5 58-59 31.50109461609926 35.0 31.0 39.0 6.0 40.0 60-61 31.0688103359943 35.0 30.5 38.5 2.0 40.0 62-63 30.6519437865564 35.0 29.5 38.0 2.0 40.0 64-65 30.26170433991378 35.0 29.0 38.0 2.0 40.0 66-67 29.810659598046904 34.5 28.5 37.0 2.0 39.5 68-69 29.3380406019533 34.0 28.0 36.5 2.0 39.0 70-71 28.796803519681447 34.0 26.5 36.0 2.0 39.0 72-73 28.445132808387328 33.5 26.0 35.5 2.0 38.5 74-75 28.02646253292656 33.0 26.0 35.0 2.0 38.0 76-77 26.13096842573571 30.5 24.0 34.0 2.0 36.0 78-79 27.249380220815524 33.0 26.0 35.0 2.0 36.5 80-81 27.301957525875718 33.0 26.0 35.0 2.0 36.0 82-83 26.926923932485085 33.0 25.5 35.0 2.0 36.0 84-85 26.449336134208487 33.0 24.5 35.0 2.0 35.5 86-87 25.80372551960294 32.5 22.0 35.0 2.0 35.0 88-89 25.111822961069915 32.0 19.0 35.0 2.0 35.0 90-91 24.482407632015736 31.0 15.5 34.0 2.0 35.0 92-93 24.04538051875241 31.0 8.5 34.0 2.0 35.0 94-95 23.48604075092174 31.0 2.0 34.0 2.0 35.0 96-97 22.671945819774354 31.0 2.0 34.0 2.0 35.0 98-99 21.675805914248528 30.0 2.0 34.0 2.0 35.0 100-101 18.562872232382723 24.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05573719214692474 1101 2 0.11064130858067855 1101 3 0.045626987248848394 1101 4 0.11319786194547277 1101 5 -0.02377578179699924 1101 6 0.21295503387698744 1101 7 0.16810014020588682 1101 8 0.18460805841031203 1101 9 0.25100907417593277 1101 10-11 0.009027513478521598 1101 12-13 0.0033807911707341987 1101 14-15 0.12008916929605817 1101 16-17 0.1108015504392128 1101 18-19 0.23711554891317377 1101 20-21 0.32246201770604443 1101 22-23 0.2576485108761162 1101 24-25 0.3998820033587549 1101 26-27 0.3480287947737821 1101 28-29 0.39376815374464513 1101 30-31 0.28185744357610076 1101 32-33 0.4588419181775123 1101 34-35 0.29308419955496134 1101 36-37 0.21110898172371861 1101 38-39 0.13653123263810585 1101 40-41 0.5288215148012156 1101 42-43 0.1602328735456311 1101 44-45 0.2736203614003898 1101 46-47 0.3635854299339556 1101 48-49 0.26849871182376006 1101 50-51 0.16612130390899438 1101 52-53 0.12228022935574501 1101 54-55 0.4204858287779274 1101 56-57 0.34159922632522566 1101 58-59 0.4152266027209883 1101 60-61 0.42175130686311135 1101 62-63 0.37067369297812647 1101 64-65 0.2981753185313245 1101 66-67 0.3385816451462631 1101 68-69 0.7143265890775332 1101 70-71 0.7427603774771789 1101 72-73 0.7215299364043197 1101 74-75 0.515938503563973 1101 76-77 0.4057033649116377 1101 78-79 0.518921176443186 1101 80-81 0.6144425756419061 1101 82-83 0.7217903515155939 1101 84-85 0.503763259142648 1101 86-87 0.7257207756138193 1101 88-89 0.9835296112200425 1101 90-91 0.8989743545991224 1101 92-93 1.0297199545457758 1101 94-95 1.0630231773611811 1101 96-97 1.2080013541748293 1101 98-99 1.319425183726004 1101 100-101 1.895395131970485 1102 1 0.0723130522567601 1102 2 0.13692262730421234 1102 3 0.14685332714762822 1102 4 0.17190276250342862 1102 5 0.20659941867164378 1102 6 0.13636112812366008 1102 7 0.13468357837511746 1102 8 0.22597902382887014 1102 9 0.17782817703675136 1102 10-11 0.25212274928725975 1102 12-13 0.1690490359777499 1102 14-15 0.3442539233078321 1102 16-17 0.2958636356972093 1102 18-19 0.3087272687660416 1102 20-21 0.2142497658310063 1102 22-23 0.2687393483788654 1102 24-25 0.1792891923802813 1102 26-27 0.2875813775781353 1102 28-29 0.3585989918235981 1102 30-31 0.4958158229251879 1102 32-33 0.5109896935758087 1102 34-35 0.4772738260830849 1102 36-37 0.491318525471506 1102 38-39 0.6868342163797365 1102 40-41 0.8948321168313456 1102 42-43 0.9792637097039361 1102 44-45 0.6466082324474414 1102 46-47 0.7182646320927688 1102 48-49 0.829324978737553 1102 50-51 0.9223826477931425 1102 52-53 1.0718701689510652 1102 54-55 1.1415789710698476 1102 56-57 1.1028393949589663 1102 58-59 1.1333244794339699 1102 60-61 0.9036241401212202 1102 62-63 0.9257672770213041 1102 64-65 0.9975174932919622 1102 66-67 0.9306997463841284 1102 68-69 1.0408609544442378 1102 70-71 1.0986044584905166 1102 72-73 1.1505491525839382 1102 74-75 0.8639122097609722 1102 76-77 0.8312505122548863 1102 78-79 1.2227173562551528 1102 80-81 1.1729261204076344 1102 82-83 1.1158497574561892 1102 84-85 1.3168357351584348 1102 86-87 1.5187108554171438 1102 88-89 1.7964061801572448 1102 90-91 1.6895348024964818 1102 92-93 1.537895713925785 1102 94-95 1.5151344602644485 1102 96-97 1.5428660918610149 1102 98-99 1.854393156759972 1102 100-101 1.764583462054702 1103 1 -0.05153266210487928 1103 2 0.047373788090862234 1103 3 -0.19892746819669682 1103 4 -0.08555821718493917 1103 5 0.033124878914868816 1103 6 0.026922986445235608 1103 7 0.11263328236550052 1103 8 0.08460999598175789 1103 9 0.33462690758354086 1103 10-11 0.020982329312353443 1103 12-13 0.09482835079949581 1103 14-15 0.2465157062746144 1103 16-17 -0.05222667997678343 1103 18-19 0.21752185764579934 1103 20-21 0.1685946250959418 1103 22-23 0.005026007921308917 1103 24-25 0.1975162286208132 1103 26-27 0.28201186039932935 1103 28-29 0.4287083796654798 1103 30-31 0.15540571816872273 1103 32-33 0.14119548621533085 1103 34-35 -0.018615825633467864 1103 36-37 -0.2512779125430029 1103 38-39 0.14693792888503054 1103 40-41 0.23352206313393253 1103 42-43 0.49566524261424405 1103 44-45 0.4164358464714013 1103 46-47 0.37514498119239903 1103 48-49 0.4568335629213962 1103 50-51 0.4311229508447241 1103 52-53 0.35090881628488546 1103 54-55 0.18981601032837148 1103 56-57 0.2491131284003636 1103 58-59 0.19422235943951804 1103 60-61 0.21623600973653012 1103 62-63 0.31653697793676017 1103 64-65 0.37618582016856905 1103 66-67 0.33961533951374534 1103 68-69 0.37900756793863266 1103 70-71 0.42077281668587574 1103 72-73 0.4032150422925973 1103 74-75 0.5867441457720268 1103 76-77 0.23900731174467538 1103 78-79 0.20454536508514565 1103 80-81 0.2794112838630234 1103 82-83 0.29197500207458305 1103 84-85 0.3046593859373239 1103 86-87 0.591221347197397 1103 88-89 0.6187410971435874 1103 90-91 0.4351459071751229 1103 92-93 0.7355791706043675 1103 94-95 0.7807345179668665 1103 96-97 0.7268694249349394 1103 98-99 0.9809023881979186 1103 100-101 1.381351614115765 1104 1 0.14228260983327345 1104 2 0.2185621006598879 1104 3 0.023844809031025704 1104 4 0.08547508966825035 1104 5 0.12473906968815385 1104 6 0.16444018239183578 1104 7 -0.05071174098222997 1104 8 0.036093206925166044 1104 9 0.0034843217006894633 1104 10-11 0.2679384045676372 1104 12-13 0.1429847515667717 1104 14-15 0.16414857523665205 1104 16-17 0.24793026331050072 1104 18-19 0.17671950930920843 1104 20-21 0.17048181968623766 1104 22-23 0.2081435603810604 1104 24-25 0.19493150830925288 1104 26-27 0.1401080026945749 1104 28-29 0.2992136982990985 1104 30-31 0.1962138792196626 1104 32-33 0.035574591444841985 1104 34-35 0.3465495460896122 1104 36-37 0.20318818964451424 1104 38-39 0.2904916286777137 1104 40-41 0.2525838910388316 1104 42-43 0.38449029928820977 1104 44-45 0.16074907427167773 1104 46-47 0.27398146953790814 1104 48-49 0.4744393058831733 1104 50-51 0.5161213039090029 1104 52-53 0.36386438777158503 1104 54-55 0.26405018521356993 1104 56-57 0.4391239788004775 1104 58-59 0.38502858291900566 1104 60-61 0.2885829900314256 1104 62-63 0.03998062367119459 1104 64-65 0.4947099719966701 1104 66-67 0.2742252095027027 1104 68-69 0.44898005442407296 1104 70-71 0.3100871101504481 1104 72-73 0.35420320373104985 1104 74-75 0.16742365207882415 1104 76-77 0.2581786124363923 1104 78-79 0.3347627606016026 1104 80-81 0.2768188132656739 1104 82-83 0.27377054953539925 1104 84-85 0.503763259142648 1104 86-87 0.6786910726435202 1104 88-89 0.6988761458735091 1104 90-91 0.8212515823218958 1104 92-93 1.0198189644467632 1104 94-95 0.8338152565691033 1104 96-97 0.9916647205114621 1104 98-99 1.2283360748151146 1104 100-101 1.7612367161288986 1105 1 0.005867872742033597 1105 2 -0.02299444543296758 1105 3 0.07796496208218784 1105 4 0.03208666317619446 1105 5 0.021467009414394056 1105 6 -0.020548575448088968 1105 7 -0.0471951144191749 1105 8 0.08012500054277183 1105 9 0.08793266693361801 1105 10-11 0.17923776159496896 1105 12-13 0.09876953323218629 1105 14-15 0.018391997586853392 1105 16-17 -0.07240818487694867 1105 18-19 -0.053957781527444126 1105 20-21 0.040964246414965544 1105 22-23 -0.09385537457219328 1105 24-25 -0.11166290611227225 1105 26-27 -0.09446657030056826 1105 28-29 0.043315508878563946 1105 30-31 -0.05268754478727544 1105 32-33 -0.04407907113257181 1105 34-35 0.028670566956641608 1105 36-37 0.07269235121605533 1105 38-39 0.12924256552437896 1105 40-41 0.26328169388685296 1105 42-43 0.17227874944796184 1105 44-45 0.29132084658834856 1105 46-47 0.24913629577751095 1105 48-49 0.07528246788860571 1105 50-51 0.2219820194626294 1105 52-53 0.12518938481312603 1105 54-55 0.4437649893633022 1105 56-57 0.5789103777590974 1105 58-59 0.49260027634332815 1105 60-61 0.5624568017153884 1105 62-63 0.5722811246376267 1105 64-65 0.7709209695904526 1105 66-67 0.8587228624552452 1105 68-69 0.7537668478444495 1105 70-71 0.6367201818241455 1105 72-73 0.5660607241444495 1105 74-75 0.5374208908465228 1105 76-77 0.20643213300757068 1105 78-79 0.11443516011247112 1105 80-81 0.21872643032219585 1105 82-83 0.1636585223964282 1105 84-85 0.23532907508679202 1105 86-87 0.2311781540209843 1105 88-89 0.2673014545398331 1105 90-91 0.36786512813862515 1105 92-93 0.526040612507984 1105 94-95 0.40839121018040103 1105 96-97 0.5216053540170442 1105 98-99 0.8004860886212732 1105 100-101 1.1331204682491318 1106 1 -0.020072018486700927 1106 2 -0.10303934519898306 1106 3 -0.11857032533955447 1106 4 -0.1614170995506754 1106 5 -0.07456157466199897 1106 6 -0.22303624382221443 1106 7 0.04139496968883094 1106 8 -0.21383803619914943 1106 9 -0.12654671711747767 1106 10-11 -0.08690797292725705 1106 12-13 0.037200333839287225 1106 14-15 0.05656272736614909 1106 16-17 -0.10759877173585863 1106 18-19 -0.3383598557701575 1106 20-21 -0.3404426656194275 1106 22-23 -0.4146100542661131 1106 24-25 -0.2902936213465779 1106 26-27 -0.38155630659349526 1106 28-29 -0.5679892719979307 1106 30-31 -0.21281291631703425 1106 32-33 -0.12529587060136294 1106 34-35 -0.07876477105638457 1106 36-37 -0.009615322849590768 1106 38-39 0.09624756141383983 1106 40-41 -0.06231670616374174 1106 42-43 -0.09949131315875093 1106 44-45 0.2135248875672886 1106 46-47 0.32228022798518197 1106 48-49 0.34604860967068873 1106 50-51 0.29743220285603655 1106 52-53 0.24014424790046007 1106 54-55 0.27411422733207047 1106 56-57 -0.05001439932305374 1106 58-59 0.1287461689633247 1106 60-61 0.2912183428524244 1106 62-63 0.21389917624073718 1106 64-65 0.20397446987503187 1106 66-67 0.14288779004646912 1106 68-69 0.0015613839855994627 1106 70-71 -0.35345799436000647 1106 72-73 -0.3104115613168794 1106 74-75 -0.14425715885941415 1106 76-77 -0.13251367107767464 1106 78-79 -0.12446637659783022 1106 80-81 -0.25486926625656636 1106 82-83 -0.23782087746444347 1106 84-85 -0.3952142278917705 1106 86-87 -0.29958371912136883 1106 88-89 0.09031100364499878 1106 90-91 0.14873174105205322 1106 92-93 0.3765473158533368 1106 94-95 0.09832393958026131 1106 96-97 0.21324553930763201 1106 98-99 0.784202018552346 1106 100-101 1.2400301311846889 1107 1 -0.13398471689940195 1107 2 -0.05330918646882665 1107 3 -0.10492746819669563 1107 4 -0.09386154399511781 1107 5 -0.06873617783659824 1107 6 -0.2604410057269746 1107 7 -0.008493919200056155 1107 8 -0.07656025842137382 1107 9 -0.20287211394287397 1107 10-11 0.005012661993376355 1107 12-13 -0.03016871377976571 1107 14-15 -0.37308409803067377 1107 16-17 -0.12893607332316748 1107 18-19 -0.05928049069078867 1107 20-21 -0.13207758625435417 1107 22-23 -0.13553465744072213 1107 24-25 -0.1121031451560981 1107 26-27 -0.392080116117306 1107 28-29 -0.2244892719979319 1107 30-31 -0.3007811702852905 1107 32-33 -0.10121253726804014 1107 34-35 -0.28703461232623084 1107 36-37 -0.25234151332578136 1107 38-39 -0.32599847033219476 1107 40-41 -0.33751511886215013 1107 42-43 -0.47677702744446293 1107 44-45 -0.41176082671842096 1107 46-47 -0.31709278788783024 1107 48-49 -0.5816498030277231 1107 50-51 -0.6465717653979333 1107 52-53 -0.5976851171789086 1107 54-55 -0.6017270425092036 1107 56-57 -0.6316334469421001 1107 58-59 -0.7186347834176274 1107 60-61 -0.9191348317507497 1107 62-63 -0.8099698713783106 1107 64-65 -0.7585969586963976 1107 66-67 -0.5197153845567044 1107 68-69 -0.22601004458583063 1107 70-71 -0.6481802165822259 1107 72-73 -0.5624552121105317 1107 74-75 -0.5334476350498889 1107 76-77 -0.6154104964744995 1107 78-79 -0.7482520908835468 1107 80-81 -0.5258891075264067 1107 82-83 -0.4016502425438091 1107 84-85 -0.5160832755108196 1107 86-87 -0.5928138778515297 1107 88-89 -0.7752921709581777 1107 90-91 -0.6232484176781057 1107 92-93 -0.276270144464128 1107 94-95 -0.18556098105466035 1107 96-97 0.07743204724414099 1107 98-99 0.24240043125075772 1107 100-101 0.5943555280100874 1108 1 -0.16448225384521464 1108 2 -0.24307765937522774 1108 3 -0.14129200021640287 1108 4 -0.07889110064536453 1108 5 -0.13315489704842065 1108 6 -0.05384494661367256 1108 7 -0.21732150540695017 1108 8 -0.21580163773171535 1108 9 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-1.8569794211210962 2316 86-87 -2.1578588457316137 2316 88-89 -2.513628359395007 2316 90-91 -3.0451295739950197 2316 92-93 -3.269664148746781 2316 94-95 -2.967319011033247 2316 96-97 -3.018579730057791 2316 98-99 -3.660968947764232 2316 100-101 -3.738010639013467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 34.0 5 161.0 6 490.0 7 865.0 8 1326.0 9 2023.0 10 3060.0 11 4318.0 12 5673.0 13 7549.0 14 8973.0 15 11756.0 16 14770.0 17 19144.0 18 16741.0 19 11029.0 20 9894.0 21 10343.0 22 11011.0 23 12568.0 24 14254.0 25 16239.0 26 18714.0 27 22477.0 28 26533.0 29 31149.0 30 36451.0 31 42683.0 32 51647.0 33 63659.0 34 88393.0 35 135870.0 36 117165.0 37 116871.0 38 56983.0 39 2669.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.64467706314736 22.434868838933987 16.54496597396066 15.375488123957986 2 15.913151397233413 27.20053064975405 38.119011695029386 18.767306257983147 3 21.207973032685487 32.67582554184755 30.78291976136866 15.333281664098303 4 11.885467423721614 21.37445533412716 37.23496450424208 29.505112737909144 5 9.981590318661619 43.29314876008934 32.414023567210435 14.311237354038605 6 23.791065718234243 40.614937710053056 18.144054498293404 17.4499420734193 7 22.464843950522344 39.99691997676897 21.35975979844972 16.17847627425896 8 25.34486698023853 33.721322316378775 20.22347279665103 20.710337906731663 9 26.604153602570307 19.687186398778458 21.83655947313485 31.872100525516384 10-11 23.268367658145607 30.763644452586263 25.39911967702031 20.568868212247825 12-13 27.60088334261031 29.548343782932847 26.928310949482587 15.922461924974257 14-15 23.02647968414616 29.286686680137596 30.64894476332351 17.037888872392735 16-17 16.505414820876577 32.912186190520764 31.241488227709347 19.34091076089331 18-19 16.167159287586102 30.101646157431013 37.254371056537245 16.47682349844564 20-21 18.56988208839772 27.70452094291033 37.18772600059285 16.537870968099096 22-23 17.44807996787113 28.49977705060599 37.03672575412758 17.015417227395297 24-25 17.555880780957 29.035157559292962 37.65771279085334 15.751248868896697 26-27 17.507359109971702 28.116505694786042 38.78196853235014 15.59416666289212 28-29 16.081898421639078 29.651615150090738 38.711261913700966 15.555224514569217 30-31 16.365240428022577 29.813769314312346 37.72942901804632 16.091561239618763 32-33 16.167052005085058 30.113618634744473 37.625352416056785 16.093976944113685 34-35 17.042100685706462 29.353712599201952 37.57232532201629 16.031861393075292 36-37 17.268758522482745 29.190293040016467 37.8342109382747 15.706737499226087 38-39 17.034145703097476 29.538751145068904 37.98135676823806 15.445746383595566 40-41 16.47341385561755 30.224494705721888 37.46827912557591 15.833812313084653 42-43 15.84814935231609 30.99088743113734 36.41046521108486 16.750498005461704 44-45 16.785532925307766 30.162956908695516 35.67965648146144 17.371853684535274 46-47 17.18782162745695 31.028226465992105 34.85884250603307 16.925109400517876 48-49 17.29700648767965 30.93889925349661 34.661904371269806 17.10218988755393 50-51 16.866483340614035 31.499636635953514 34.94711593628145 16.686764087151005 52-53 16.98798891594254 32.274566959805696 33.7963098100753 16.941134314176463 54-55 16.0137554240115 31.464198756113493 34.26002441874627 18.262021401128738 56-57 17.38899586209951 31.590620624681055 33.256248873929415 17.764134639290024 58-59 18.103293823024956 30.91386376995982 33.305670078188534 17.67717232882669 60-61 17.06014329225944 31.338588859117415 34.527586477623416 17.073681370999726 62-63 17.631220564892367 31.034907936495156 34.35730685184384 16.976564646768644 64-65 17.153816964083507 32.130228616234135 33.948700501963266 16.767253917719092 66-67 17.183861449272737 31.437933858052137 33.5222139852207 17.85599070745442 68-69 16.942722537804915 31.930414896599206 33.355695611977396 17.771166953618486 70-71 17.506146308566077 31.947568276240396 32.92574353347945 17.62054188171407 72-73 17.357943993904374 30.94290985698023 33.43818161870319 18.26096453041221 74-75 16.71878884628973 32.7070787187506 32.87919766401375 17.69493477094592 76-77 17.099722779617853 31.18492573878426 33.564165639178334 18.151185842419554 78-79 16.870727682252834 31.029373710564283 34.02469071163259 18.075207895550296 80-81 16.81653268726626 31.81474355522429 33.441620696961294 17.927103060548156 82-83 16.63982550561198 31.4762772785881 33.455745803468346 18.428151412331566 84-85 16.41818461820294 31.386141304621347 33.24623376571088 18.949440311464834 86-87 16.81794039956019 31.444124965608154 32.81511245755819 18.922822177273467 88-89 17.010242932084104 30.92388289551987 32.91962177924066 19.14625239315536 90-91 17.042963988472437 30.491384599239566 33.248151392106685 19.21750002018131 92-93 16.88431493136883 31.56414852959087 33.182066615064535 18.369469923975768 94-95 16.616171732432544 31.311002829393892 32.90828677230677 19.1645386658668 96-97 16.049538046842525 31.032039287407436 33.15690302657577 19.76151963917427 98-99 16.932126551088277 31.015373992446875 32.211503619541496 19.84099583692335 100-101 17.051126100849075 30.299865776811046 32.98171253086957 19.66729559147031 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23111.0 1 14459.5 2 8776.5 3 10689.0 4 7520.0 5 6002.0 6 9704.5 7 18787.5 8 24925.0 9 23288.5 10 16169.5 11 9192.0 12 7269.0 13 6667.5 14 6015.0 15 5354.0 16 4750.5 17 4424.5 18 4273.5 19 4267.0 20 4368.0 21 4587.0 22 4900.0 23 5295.0 24 5778.0 25 6529.0 26 7525.5 27 8536.5 28 9829.0 29 11165.5 30 12505.5 31 14089.0 32 16375.0 33 18499.0 34 20285.0 35 22015.5 36 24280.0 37 26392.5 38 26563.5 39 26108.0 40 25271.0 41 24268.0 42 23871.0 43 24129.0 44 24898.5 45 25741.5 46 26501.5 47 33508.5 48 54714.5 49 53885.5 50 34639.0 51 25355.0 52 17818.0 53 14993.0 54 13765.5 55 12564.5 56 11388.0 57 10392.5 58 9661.0 59 8988.0 60 8105.5 61 7197.0 62 6118.5 63 4349.0 64 2682.0 65 1926.5 66 1520.5 67 1176.0 68 891.5 69 693.5 70 549.0 71 440.0 72 369.5 73 352.5 74 314.5 75 258.5 76 186.5 77 112.0 78 71.0 79 44.0 80 28.0 81 15.5 82 9.0 83 6.0 84 4.5 85 3.5 86 1.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3249122545669397 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.5098153093259277E-4 16-17 0.0022143957870113607 18-19 3.522902388427165E-4 20-21 5.0327176977530925E-5 22-23 0.0 24-25 0.0 26-27 2.5163588488765463E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007146459130809393 36-37 0.017161567349338047 38-39 0.010971324581101742 40-41 0.006844496068944206 42-43 0.0036235567423822273 44-45 0.0015601424863034587 46-47 0.0016607968402585205 48-49 5.0327176977530925E-5 50-51 5.032717697753093E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.0196306186518554E-4 60-61 5.032717697753093E-4 62-63 0.0 64-65 0.0 66-67 0.0014594881323483969 68-69 0.0010568707165281494 70-71 0.0016607968402585205 72-73 0.0 74-75 0.0 76-77 0.07921497656263368 78-79 0.033417245513080535 80-81 5.0327176977530925E-5 82-83 0.0 84-85 0.0 86-87 0.12662317727546782 88-89 0.2932564602480727 90-91 0.2499247608704186 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.0196306186518554E-4 100-101 4.529445927977784E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 993499.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.668470793448904 #Duplication Level Percentage of deduplicated Percentage of total 1 75.16111150095699 30.566874678798268 2 12.999683960217748 10.573545349203634 3 4.66444591072529 5.690876468638609 4 2.2823364850515375 3.712765387325652 5 1.2586223815176827 2.55931237813665 6 0.770639462003386 1.8804437071658329 7 0.5306371678859776 1.5106141514862526 8 0.37660132036630956 1.2252639838473245 9 0.2594233395856432 0.9495315458169938 >10 1.4847827685850297 11.169115403253846 >50 0.12613948167764488 3.553102941053201 >100 0.06925243352737961 5.091631809100497 >500 0.0066779132315510185 1.9294287109813422 >1k 0.006925243351238092 5.5145933207982525 >5k 0.0014839807181224484 4.019894611321517 >10k+ 0.0012366505984353737 10.053005553072165 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26061 2.623153118422867 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23084 2.323505106698648 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21385 2.152493359328998 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19014 1.913841886101546 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10401 1.0469059354865984 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9254 0.9314553915001424 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8487 0.85425350201661 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6428 0.6470061872231376 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5388 0.5423256591098733 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5365 0.5400106089689068 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5043 0.5075999069953769 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4809 0.4840467881698925 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3130 0.3150481278793436 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2778 0.27961779528716185 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2633 0.2650229139636779 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2582 0.2598895419119697 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2545 0.2561653308156324 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2451 0.24670382154385662 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2255 0.22697556816866446 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2104 0.21177676072145013 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2095 0.21087087153585457 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1978 0.19909431212311235 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1951 0.19637664456632567 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1944 0.19567206408864024 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1878 0.18902887672760615 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1857 0.18691513529454987 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1673 0.1683947341668185 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1626 0.1636639795309306 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1597 0.1607450032662338 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1587 0.15973845972668316 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1344 0.13527945171560313 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1329 0.1337696364062772 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1314 0.13225982109695128 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1313 0.13215916674299621 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1289 0.12974346224807473 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1263 0.12712644904524312 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1229 0.12370420101077102 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1217 0.12249634876331028 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1054 0.1060896890686352 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.0065435395506186E-4 0.0 14-15 0.0 0.0 0.0 1.0065435395506186E-4 0.0 16-17 0.0 0.0 0.0 1.509815309325928E-4 0.0 18-19 0.0 0.0 0.0 2.0130870791012373E-4 0.0 20-21 0.0 0.0 0.0 0.0010568707165281494 0.0 22-23 0.0 0.0 0.0 0.0029189762646967938 0.0 24-25 0.0 0.0 0.0 0.004982390520775562 0.0 26-27 0.0 0.0 0.0 0.007800712431517294 0.0 28-29 0.0 0.0 0.0 0.013336701899045695 0.0 30-31 0.0 0.0 0.0 0.029491725708833124 0.0 32-33 0.0 0.0 0.0 0.049622596499845496 0.0 34-35 0.0 0.0 0.0 0.07916464938565615 0.0 36-37 0.0 0.0 0.0 0.12330158359495078 0.0 38-39 0.0 0.0 0.0 0.18585826457802174 0.0 40-41 0.0 0.0 0.0 0.25963790602708203 0.0 42-43 0.0 0.0 0.0 0.33130380604308607 0.0 44-45 1.0065435395506186E-4 0.0 0.0 0.41776589609048426 0.0 46-47 1.0065435395506186E-4 0.0 0.0 0.5266739070698612 0.0 48-49 1.0065435395506186E-4 0.0 0.0 0.6481133851166433 0.0 50-51 1.0065435395506186E-4 0.0 0.0 0.7797189529128867 0.0 52-53 1.0065435395506186E-4 0.0 0.0 0.9091604520990962 0.0 54-55 1.0065435395506186E-4 0.0 0.0 1.0423764895586207 0.0 56-57 1.0065435395506186E-4 0.0 0.0 1.200907097037843 0.0 58-59 1.0065435395506186E-4 0.0 0.0 1.3882248497482133 0.0 60-61 1.0065435395506186E-4 0.0 0.0 1.6150997635629225 0.0 62-63 1.0065435395506186E-4 0.0 0.0 1.8364386879101036 0.0 64-65 1.0065435395506186E-4 0.0 0.0 2.0422265145712277 0.0 66-67 1.0065435395506186E-4 0.0 0.0 2.274788399384398 0.0 68-69 1.0065435395506186E-4 0.0 0.0 2.546001556116312 0.0 70-71 1.0065435395506186E-4 0.0 0.0 2.8589359425625993 0.0 72-73 1.0065435395506186E-4 0.0 0.0 3.1768527195296623 0.0 74-75 1.0065435395506186E-4 0.0 0.0 3.4898877603299048 0.0 76-77 1.0065435395506186E-4 0.0 0.0 3.8018156032366415 0.0 78-79 1.0065435395506186E-4 0.0 0.0 4.180426955638607 0.0 80-81 1.0065435395506186E-4 0.0 0.0 4.6060942185145635 0.0 82-83 1.0065435395506186E-4 0.0 0.0 5.026275819099969 0.0 84-85 1.0065435395506186E-4 0.0 0.0 5.450080976427756 0.0 86-87 1.0065435395506186E-4 0.0 0.0 5.893866023015624 0.0 88-89 1.0065435395506186E-4 0.0 0.0 6.348723048538549 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 45 9.6671644E-4 42.184242 2 ATCACGC 50 0.0016228693 37.965816 3 GAGCGGT 60 0.003968139 31.638182 7 TAGGGTC 160 1.9226718E-9 29.660791 4 TCACGCA 65 0.005869451 29.204473 4 CTCGATT 35 0.00842994 27.11844 22-23 GTAAGCG 135 1.8189894E-12 24.609331 94-95 CCGGTCA 50 0.0015994735 23.887331 84-85 ATACGTC 140 1.7916007E-5 23.728634 5 GTGTAGC 290 3.6379788E-12 22.98096 1 CGTCACT 105 0.0021684277 22.668295 1 GAGTACT 15035 0.0 22.585558 12-13 CATATAG 300 7.2759576E-12 22.146727 3 TACCTGG 580 0.0 22.092178 2 AGTACTT 15270 0.0 21.709608 12-13 GTACTTT 16495 0.0 20.629631 14-15 TGCACTG 490 0.0 20.338829 5 CATGGGT 635 0.0 20.17868 4 CATGGGG 1855 0.0 19.44341 4 ACTTTTT 17690 0.0 19.383608 16-17 >>END_MODULE