##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938698_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 974788 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.273573330816546 33.0 31.0 34.0 31.0 34.0 2 32.29669630729964 34.0 31.0 34.0 31.0 34.0 3 32.29548373595079 34.0 31.0 34.0 31.0 34.0 4 35.85244176169588 37.0 35.0 37.0 35.0 37.0 5 35.90905612297238 37.0 35.0 37.0 35.0 37.0 6 36.04083759750838 37.0 37.0 37.0 35.0 37.0 7 36.01211442898353 37.0 37.0 37.0 35.0 37.0 8 36.00332482550052 37.0 37.0 37.0 35.0 37.0 9 37.656238074330005 39.0 38.0 39.0 35.0 39.0 10-11 37.587727280188105 39.0 38.0 39.0 35.0 39.0 12-13 37.48631497310184 39.0 37.5 39.0 35.0 39.0 14-15 38.633706508492104 40.0 38.0 41.0 35.0 41.0 16-17 38.582290713467955 40.0 38.0 41.0 35.5 41.0 18-19 38.61727985982593 40.0 38.0 41.0 35.0 41.0 20-21 38.57138116185263 40.0 38.0 41.0 35.0 41.0 22-23 38.51676980020271 40.0 38.0 41.0 35.0 41.0 24-25 38.42088074535181 40.0 38.0 41.0 35.0 41.0 26-27 38.22883385925965 40.0 38.0 41.0 35.0 41.0 28-29 38.01855070025482 40.0 37.0 41.0 34.5 41.0 30-31 37.634951394559636 39.5 36.5 41.0 34.0 41.0 32-33 37.26173947566035 39.0 35.0 41.0 33.5 41.0 34-35 36.92782584520942 39.0 35.0 41.0 33.0 41.0 36-37 36.66489328961785 39.0 35.0 41.0 33.0 41.0 38-39 36.462136895407 38.0 35.0 41.0 33.0 41.0 40-41 36.27018849226704 38.0 35.0 40.5 32.5 41.0 42-43 36.09335004123973 39.0 35.0 41.0 31.5 41.0 44-45 35.76756228020862 38.5 35.0 41.0 30.5 41.0 46-47 35.26695086521377 38.0 35.0 41.0 28.0 41.0 48-49 34.85120969892941 38.0 35.0 41.0 24.5 41.0 50-51 33.69191095910085 37.0 34.0 39.5 23.0 40.5 52-53 33.84279915222592 37.5 34.0 39.5 23.0 40.5 54-55 34.33013691182083 38.0 35.0 40.0 23.0 41.0 56-57 34.230054124589145 38.0 35.0 40.0 23.0 41.0 58-59 34.052639650877936 37.5 35.0 40.0 22.5 41.0 60-61 33.75843568037358 37.0 35.0 40.0 21.5 41.0 62-63 33.41760310959921 36.0 34.0 40.0 19.5 41.0 64-65 33.05501555209953 36.0 34.0 39.5 15.5 41.0 66-67 32.66836378781848 35.0 33.5 39.0 9.5 41.0 68-69 32.29545603762049 35.0 33.0 39.0 4.5 40.5 70-71 31.866219629293752 35.0 33.0 38.0 2.0 40.0 72-73 31.451322236219568 35.0 33.0 37.0 2.0 40.0 74-75 31.022126862456247 35.0 32.0 37.0 2.0 39.0 76-77 30.568541570064465 35.0 31.5 36.0 2.0 39.0 78-79 30.15523529218661 35.0 31.0 36.0 2.0 38.5 80-81 29.704509083000612 35.0 31.0 35.0 2.0 37.0 82-83 29.31649035482587 35.0 30.0 35.0 2.0 37.0 84-85 29.00030262990517 34.5 30.0 35.0 2.0 36.5 86-87 28.61718291566987 34.0 29.5 35.0 2.0 36.0 88-89 28.148693356914528 34.0 29.0 35.0 2.0 36.0 90-91 27.677956129948257 34.0 28.0 35.0 2.0 35.0 92-93 27.260304291804985 34.0 27.0 35.0 2.0 35.0 94-95 26.791087395413157 34.0 25.5 35.0 2.0 35.0 96-97 26.235276285715457 34.0 24.5 35.0 2.0 35.0 98-99 25.590975678814267 33.0 20.5 35.0 2.0 35.0 100-101 23.27802352921866 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08410244368464959 1101 2 0.16577496120109458 1101 3 0.2257309299844792 1101 4 0.15525668175362028 1101 5 -0.02201421548804916 1101 6 0.1372569820307632 1101 7 0.1447278030357424 1101 8 0.13995377645111517 1101 9 0.27306234051886236 1101 10-11 0.2521262933065742 1101 12-13 0.1759895786222856 1101 14-15 0.2023538523547117 1101 16-17 0.2787807193744243 1101 18-19 0.29554465247307604 1101 20-21 0.3473448019759431 1101 22-23 0.43370625198112833 1101 24-25 0.26542525688020646 1101 26-27 0.3594515607774653 1101 28-29 0.39608901994347434 1101 30-31 0.388366553754274 1101 32-33 0.13996863464119258 1101 34-35 0.3298061658941407 1101 36-37 0.24966701313984885 1101 38-39 0.02910389882224962 1101 40-41 0.14658073635036573 1101 42-43 0.31644572962174067 1101 44-45 0.151359167082866 1101 46-47 0.08079566495772639 1101 48-49 0.09828098851662759 1101 50-51 0.32719948877304716 1101 52-53 0.3195578995258188 1101 54-55 0.19899943355091665 1101 56-57 0.17747788754154925 1101 58-59 0.2366697626980354 1101 60-61 0.13566266581602804 1101 62-63 -0.011564514582396157 1101 64-65 -0.021405072265153535 1101 66-67 0.11684918768447972 1101 68-69 0.08031734369097876 1101 70-71 0.18465016051338168 1101 72-73 0.24295141029051237 1101 74-75 0.20223467083780733 1101 76-77 0.20838363612735833 1101 78-79 0.03200814977080313 1101 80-81 0.0767918428202492 1101 82-83 0.13187511097299875 1101 84-85 0.05039722806356295 1101 86-87 -0.01937569494224789 1101 88-89 0.021885091447110483 1101 90-91 0.1478863293152486 1101 92-93 0.2800622389574201 1101 94-95 0.11558172780449993 1101 96-97 -0.01797888728679098 1101 98-99 0.13022896355941427 1101 100-101 0.11471123180237086 1102 1 0.08300721526742194 1102 2 0.16601583617945437 1102 3 0.11540642622456687 1102 4 0.24409250287383344 1102 5 0.2174625588949013 1102 6 0.165493927736037 1102 7 0.1801345430187311 1102 8 0.1507743321195818 1102 9 0.244099006206973 1102 10-11 0.3390417010251099 1102 12-13 0.2794896049679849 1102 14-15 0.3211720218554248 1102 16-17 0.3138393197402536 1102 18-19 0.39568183830058956 1102 20-21 0.3140588909039721 1102 22-23 0.27478428041943204 1102 24-25 0.20377255343494483 1102 26-27 0.1641320627007019 1102 28-29 0.2597951825792535 1102 30-31 0.264670665941793 1102 32-33 0.3693164280005732 1102 34-35 0.47213316873194344 1102 36-37 0.5454633684003412 1102 38-39 0.3479207510379254 1102 40-41 0.5384090527095466 1102 42-43 0.5211377107431545 1102 44-45 0.5328976054602705 1102 46-47 0.6884658393662662 1102 48-49 0.6057302354059573 1102 50-51 0.6539398104916927 1102 52-53 0.7060060474230596 1102 54-55 0.5444913077840638 1102 56-57 0.6052360942273296 1102 58-59 0.5006235749209296 1102 60-61 0.5366498768601389 1102 62-63 0.3865265512141107 1102 64-65 0.40224132326662243 1102 66-67 0.4425375632085675 1102 68-69 0.7239475177984218 1102 70-71 0.5348983746512381 1102 72-73 0.6201446898786109 1102 74-75 0.6666068151520292 1102 76-77 0.6066572399934032 1102 78-79 0.5978724016658106 1102 80-81 0.6595502753263283 1102 82-83 0.6969668692850668 1102 84-85 0.870617929762485 1102 86-87 0.7473453567781263 1102 88-89 0.6204612945047145 1102 90-91 0.3515480053534574 1102 92-93 0.36709620232936757 1102 94-95 0.45970927110553816 1102 96-97 0.5429860051851172 1102 98-99 0.3339263944772206 1102 100-101 0.7214113198722885 1103 1 0.22530999550123454 1103 2 0.3150481576255686 1103 3 0.34658499648359964 1103 4 0.137836158111746 1103 5 0.3341652474366654 1103 6 0.24733726532650024 1103 7 0.19370005371067833 1103 8 0.2615252244796693 1103 9 0.3892753496591794 1103 10-11 0.34728989174456615 1103 12-13 0.3015243497038895 1103 14-15 0.3705588312997037 1103 16-17 0.396869741851134 1103 18-19 0.4624492024817499 1103 20-21 0.5589830812304584 1103 22-23 0.3800131830621325 1103 24-25 0.357970739242333 1103 26-27 0.47554935975018253 1103 28-29 0.5128734155425434 1103 30-31 0.35811059127826894 1103 32-33 0.3178256135787194 1103 34-35 0.5167675466996542 1103 36-37 0.47246995057933105 1103 38-39 0.5722797253968963 1103 40-41 0.3458066359691969 1103 42-43 0.5698040826853941 1103 44-45 0.649895018434691 1103 46-47 0.5867408827562528 1103 48-49 0.8316324196414726 1103 50-51 1.0228160917242946 1103 52-53 1.0380294943890362 1103 54-55 0.9645145582670906 1103 56-57 1.0577936473595955 1103 58-59 0.9370449653653097 1103 60-61 0.8569744012076512 1103 62-63 0.8980617380366525 1103 64-65 0.8170250282802058 1103 66-67 0.8008528856043426 1103 68-69 0.9478111913747398 1103 70-71 0.911331062865532 1103 72-73 0.8592726330295513 1103 74-75 0.7785243250580436 1103 76-77 0.7466595322945437 1103 78-79 0.7325041598607882 1103 80-81 0.8488165752248129 1103 82-83 0.7885320197582644 1103 84-85 0.8595821371174956 1103 86-87 0.8094462507755722 1103 88-89 0.9259677293214956 1103 90-91 0.8556004663024694 1103 92-93 0.9913348308783405 1103 94-95 0.9279281531175236 1103 96-97 1.0342220467085141 1103 98-99 1.2308792713151568 1103 100-101 1.4307066010066514 1104 1 0.15379172592471235 1104 2 0.07895710017528756 1104 3 0.05768965354295119 1104 4 0.09397718836280688 1104 5 0.0570061366206005 1104 6 0.07892062862507743 1104 7 -0.04931343525750265 1104 8 0.0285855883037911 1104 9 0.194240359478556 1104 10-11 0.19383190301149966 1104 12-13 0.19994956107408512 1104 14-15 0.19516766966258814 1104 16-17 0.290588566476103 1104 18-19 0.22315924037625479 1104 20-21 0.06900253558648473 1104 22-23 -0.020661726350645893 1104 24-25 -0.017337441729281977 1104 26-27 0.11176811522460639 1104 28-29 0.13251586107495683 1104 30-31 0.30555616979180655 1104 32-33 0.2968868256496364 1104 34-35 0.3317879691634431 1104 36-37 0.398760991433484 1104 38-39 0.32828752557945506 1104 40-41 0.2882844386411776 1104 42-43 -0.037647581076946324 1104 44-45 0.09392502055531082 1104 46-47 0.3306049235458559 1104 48-49 0.3197269991600038 1104 50-51 0.3527559768421753 1104 52-53 0.14832647297080825 1104 54-55 0.14497860458873646 1104 56-57 0.046109508652811826 1104 58-59 0.23965009725846897 1104 60-61 0.20868610793554154 1104 62-63 0.34035052919276154 1104 64-65 0.22708044092461677 1104 66-67 0.15744754349350387 1104 68-69 0.1330954923548333 1104 70-71 0.2903600046776482 1104 72-73 0.09984003638011885 1104 74-75 0.1273524536596362 1104 76-77 0.08234075745797043 1104 78-79 0.05318616873050175 1104 80-81 0.02603746053631184 1104 82-83 0.12330414563071557 1104 84-85 0.19863191629672272 1104 86-87 0.3355339889992308 1104 88-89 0.30597630399026343 1104 90-91 0.48636554803842813 1104 92-93 0.43246558074695685 1104 94-95 0.5354932237893912 1104 96-97 0.4177107382878198 1104 98-99 0.5528175152598713 1104 100-101 0.9912137972743622 1105 1 0.04260862786836128 1105 2 -0.10996619889563419 1105 3 -0.015000483823342847 1105 4 -0.11092657923546767 1105 5 -0.10962316438827457 1105 6 -0.03465640479502241 1105 7 0.06192856120858892 1105 8 0.015130151600580177 1105 9 -0.08675065376408497 1105 10-11 0.0115707740619726 1105 12-13 0.12060105348378158 1105 14-15 0.03620846156120905 1105 16-17 0.06014473845945645 1105 18-19 0.09624361709808227 1105 20-21 -0.0403566117165397 1105 22-23 0.12320428926692983 1105 24-25 0.01909597456847223 1105 26-27 0.02493950959695468 1105 28-29 0.14446463845796842 1105 30-31 0.23367161067491082 1105 32-33 0.1466958808051544 1105 34-35 0.267686549901768 1105 36-37 0.27251984994430245 1105 38-39 0.30459611524753427 1105 40-41 0.232508594588964 1105 42-43 0.18411823124612425 1105 44-45 0.25975539313525076 1105 46-47 0.1567263682668525 1105 48-49 0.1894712660647926 1105 50-51 0.3085508203813987 1105 52-53 0.2964436238077326 1105 54-55 0.3070746279961085 1105 56-57 0.24538087832582534 1105 58-59 0.3612848131211379 1105 60-61 0.23635221095076275 1105 62-63 0.2221186214542641 1105 64-65 0.18829488813454276 1105 66-67 0.17337766338026483 1105 68-69 0.05678880263545949 1105 70-71 0.03963422427372265 1105 72-73 0.14725515617188734 1105 74-75 -0.11124500420900318 1105 76-77 -0.08477493041845463 1105 78-79 -0.1281962339446281 1105 80-81 0.0493042323334727 1105 82-83 -0.06555740510741614 1105 84-85 0.0987278637061415 1105 86-87 0.20634412712550088 1105 88-89 0.21109381581744202 1105 90-91 0.3672710635895058 1105 92-93 0.510810915422919 1105 94-95 0.6258585839246678 1105 96-97 0.7445000149773513 1105 98-99 0.811007244137194 1105 100-101 1.103555864038558 1106 1 0.2091881900619228 1106 2 0.220337834428598 1106 3 0.13923056756635788 1106 4 0.24236722802362465 1106 5 0.2689735244803586 1106 6 0.21857952638082878 1106 7 0.16162834112462576 1106 8 0.13302702377573183 1106 9 0.1281815692775652 1106 10-11 0.15999824797418682 1106 12-13 0.19979014955227825 1106 14-15 0.3638533888870441 1106 16-17 0.4049235995587992 1106 18-19 0.34445652365820933 1106 20-21 0.4007859279789088 1106 22-23 0.3468692196511043 1106 24-25 0.24347661337332482 1106 26-27 0.18452555042766505 1106 28-29 -0.06641819441178143 1106 30-31 -0.1218491676132416 1106 32-33 -0.01696181022947485 1106 34-35 -0.05869439473656257 1106 36-37 0.025015877012499743 1106 38-39 -0.040720857930097054 1106 40-41 0.012993224520776891 1106 42-43 -0.12367026360794142 1106 44-45 0.022781867088113472 1106 46-47 -0.1734595901049545 1106 48-49 -0.3160056696385567 1106 50-51 -0.2226490340573548 1106 52-53 -0.16502184120433583 1106 54-55 -0.172827950489868 1106 56-57 -0.24160668121975704 1106 58-59 -0.10556467615629828 1106 60-61 0.033082792944142625 1106 62-63 0.015569720035237822 1106 64-65 -0.15065668371406105 1106 66-67 -0.30486315532435526 1106 68-69 -0.22956812052485276 1106 70-71 -0.31697100470458395 1106 72-73 -0.12608642305156437 1106 74-75 -0.21316256079303386 1106 76-77 -0.2105687909080629 1106 78-79 -0.11806549980877179 1106 80-81 0.01537897571401814 1106 82-83 -0.1187598494524984 1106 84-85 0.06245030239911031 1106 86-87 -0.023282620528970455 1106 88-89 0.23909596826575807 1106 90-91 0.47355932906845766 1106 92-93 0.6344697484939736 1106 94-95 0.8382521954890443 1106 96-97 0.9936991377385205 1106 98-99 1.1245939934176157 1106 100-101 1.573614669236182 1107 1 0.12132139151263033 1107 2 0.030070172647583604 1107 3 0.1047132879452235 1107 4 0.2100799636888837 1107 5 0.20198014858490154 1107 6 0.07146745612335792 1107 7 0.059218485895776496 1107 8 0.01266914229025673 1107 9 0.15801401839785 1107 10-11 0.15862546974680924 1107 12-13 0.0893729208857863 1107 14-15 0.1842772116847513 1107 16-17 0.20971183802656412 1107 18-19 0.3872614272939714 1107 20-21 0.35554269445673725 1107 22-23 0.32798003102897866 1107 24-25 0.13536433330259 1107 26-27 0.1464204639546267 1107 28-29 0.17903934837514157 1107 30-31 0.046131829299504545 1107 32-33 0.12317396892707677 1107 34-35 0.08324427984305771 1107 36-37 0.03604156415094906 1107 38-39 0.03478943790661049 1107 40-41 0.043320233482795345 1107 42-43 0.14338605626736722 1107 44-45 0.21149391336692247 1107 46-47 0.6204842998330022 1107 48-49 0.590831245506962 1107 50-51 0.547818250059791 1107 52-53 0.47446651415938845 1107 54-55 0.4316560587419147 1107 56-57 0.48073243696504875 1107 58-59 0.8043504993375095 1107 60-61 0.6454273063272282 1107 62-63 0.5699817932900473 1107 64-65 0.628904506826359 1107 66-67 0.46138987947468024 1107 68-69 0.5286235888537973 1107 70-71 0.6654016836028269 1107 72-73 0.6753867658103481 1107 74-75 0.5831776963780833 1107 76-77 0.7818749341766136 1107 78-79 0.7406972013871638 1107 80-81 0.6794649392065075 1107 82-83 0.7646349291945072 1107 84-85 0.9026625135312045 1107 86-87 0.9156752070045293 1107 88-89 0.9253202286739963 1107 90-91 0.8938677545697615 1107 92-93 0.9818036213471331 1107 94-95 1.154449779639151 1107 96-97 1.2110372068570072 1107 98-99 1.2784230855256347 1107 100-101 1.7119895156229 1108 1 0.1386825026237446 1108 2 0.07058959096872996 1108 3 -0.031145297913361958 1108 4 -0.012971673371374948 1108 5 0.08851140946961067 1108 6 0.028041820456344624 1108 7 0.08111634378501265 1108 8 0.06863831679391552 1108 9 -0.06756287118766124 1108 10-11 0.06012647114005176 1108 12-13 0.04307916435496395 1108 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-1.1663285881234557 2316 50-51 -1.2430908408493053 2316 52-53 -1.4663138383856733 2316 54-55 -1.3786925251360742 2316 56-57 -1.1873602546570439 2316 58-59 -0.9729321308862922 2316 60-61 -1.0126138899492645 2316 62-63 -1.2333555012237198 2316 64-65 -1.483097869881906 2316 66-67 -1.1634368188814364 2316 68-69 -1.1902118240992579 2316 70-71 -0.9535228559099522 2316 72-73 -0.8882694037281773 2316 74-75 -1.188935593382265 2316 76-77 -1.1160157372434654 2316 78-79 -1.0494493622888115 2316 80-81 -0.9141575602983458 2316 82-83 -0.7789083467017086 2316 84-85 -0.8361471517490493 2316 86-87 -0.8343445988976335 2316 88-89 -0.7828893177708451 2316 90-91 -1.2211550222177223 2316 92-93 -0.8197511419723362 2316 94-95 -0.783735999723099 2316 96-97 -0.8887152193660093 2316 98-99 -1.2982950764866423 2316 100-101 -1.380910068453158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4198.0 3 681.0 4 568.0 5 621.0 6 714.0 7 782.0 8 986.0 9 1114.0 10 1390.0 11 2013.0 12 3350.0 13 5187.0 14 5422.0 15 7225.0 16 10232.0 17 14535.0 18 12609.0 19 6926.0 20 6155.0 21 6033.0 22 6331.0 23 6909.0 24 7660.0 25 8445.0 26 9520.0 27 11024.0 28 13101.0 29 15637.0 30 19195.0 31 24039.0 32 31146.0 33 42774.0 34 67188.0 35 140723.0 36 132541.0 37 164604.0 38 155942.0 39 27250.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.832073062140246 20.3237495141598 15.06217726329557 16.782000160404383 2 14.320907244539551 24.658699477814565 40.690706578632415 20.329686699013465 3 20.26437693442778 32.95268178996995 29.86109113030182 16.921850145300446 4 10.647994049747073 19.087141618485234 38.95299382373609 31.311870508031607 5 10.218615690946677 42.341255115372796 32.49393762149171 14.94619157218882 6 24.062418757687514 38.66748735317299 19.926035457356328 17.344058431783175 7 23.125704158751308 39.37614618541271 21.748225725540507 15.749923930295479 8 25.209951856797876 32.236001410473484 21.303392641070477 21.250654091658163 9 27.705344899311278 19.049042417145586 22.76607655526966 30.479536128273477 10-11 22.90212835489127 29.9100121213616 26.07546896412457 21.112390559622558 12-13 27.044792716334037 28.93158613871256 27.40845187669606 16.61516926825734 14-15 23.3657884571128 28.15844141434066 30.921904280501032 17.5538658480455 16-17 16.977928343479036 32.58724120234528 30.8666372528753 19.568193201300378 18-19 16.424859574743653 30.31823557316478 36.60693314529446 16.6499717067971 20-21 18.87492960988462 28.18836394875189 35.81228137960516 17.124425061758338 22-23 17.462912808798635 29.029089434346123 35.83459737247768 17.673400384377555 24-25 17.60360298316151 29.283746230831653 36.42115185065074 16.691498935356098 26-27 17.50957028506871 28.579821656496605 37.5785905481956 16.332017510239087 28-29 16.149377875064804 30.09102486030991 37.49429101621969 16.2653062484056 30-31 16.332141889310375 30.41128729947411 36.66567422516287 16.590896586052647 32-33 16.03020138193517 30.681807089083907 36.422504920620156 16.865486608360765 34-35 16.96846921480225 30.16423506020786 36.133565636908685 16.7337300880812 36-37 17.11240048715354 29.805416546130825 36.76681179010395 16.31537117661169 38-39 17.182989572670422 30.289640038145883 36.44959231977075 16.077778069412943 40-41 16.311898748764637 31.06192691583679 36.61050572627574 16.015668609122837 42-43 15.792816071694157 31.628037668869457 35.253144004327005 17.326002255109376 44-45 16.295461388217674 31.30002907881904 34.69705274588972 17.707456787073564 46-47 16.7832027460138 31.64581198411232 34.16942501604569 17.40156025382819 48-49 16.870273923874173 31.543947199218348 34.01374587075628 17.5720330061512 50-51 16.62659346938145 32.04872612530531 34.50835051307591 16.81632989223733 52-53 16.476656200133835 32.71853605806352 33.423997529211924 17.380810212590724 54-55 15.71615562511163 31.939209294640662 34.14798355154965 18.19665152869806 56-57 17.0501972545948 32.18327334556761 32.788211451985255 17.97831794785233 58-59 17.427100247578238 31.644224100496597 33.134684209118205 17.793991442806956 60-61 16.918480281900948 31.553896171695822 34.37943781313827 17.14818573326496 62-63 17.262728830743733 31.785458793739675 33.87683154660505 17.074980828911546 64-65 16.746663234442373 32.62886929660853 33.81643606409679 16.808031404852308 66-67 16.698544861243615 32.206638661908706 33.141926870820974 17.952889606026705 68-69 16.328680510145762 32.66781313877633 33.274756846652494 17.728749504425416 70-71 16.997700543404946 32.42228650220009 32.67406309758326 17.905949856811716 72-73 16.823656848430684 31.610937554701156 33.329403430444835 18.236002166423322 74-75 16.349785222129327 33.023178712485944 32.87244294898459 17.754593116400134 76-77 16.612474424470154 31.879296367309646 33.26319311294411 18.245036095276088 78-79 16.486457695963423 31.664048143095407 33.79372346847248 18.055770692468688 80-81 16.633522727272727 32.49814723320158 32.88177289196311 17.986557147562582 82-83 16.363940509922305 31.972339533004433 33.194670701986006 18.469049255087256 84-85 16.440649788017495 31.810964207717635 32.86117449168721 18.88721151257766 86-87 16.605607619016247 32.0649041034974 32.95937883441898 18.37010944306737 88-89 16.905896026593012 31.31241745017156 32.617669858336 19.164016664899428 90-91 16.549868254273886 31.075825611698647 33.116571542717224 19.25773459131024 92-93 16.846151786854254 31.834712377626122 32.98580936651826 18.333326469001364 94-95 16.563049140708223 31.597347185387587 32.85069448198703 18.988909191917163 96-97 16.050447426141222 31.17648876028462 33.12817291552965 19.644890898044505 98-99 16.835361116302632 31.263743590614894 32.164803124592936 19.736092168489535 100-101 17.252415735571088 30.330458934425515 33.066276573348645 19.350848756654756 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23110.0 1 14253.0 2 9036.5 3 11101.5 4 7399.0 5 6176.0 6 9901.5 7 18024.5 8 24665.0 9 23067.5 10 15258.5 11 9033.0 12 7564.0 13 7108.5 14 6394.0 15 5550.0 16 4782.0 17 4353.5 18 4173.5 19 4066.0 20 4133.0 21 4357.5 22 4645.5 23 5131.0 24 5572.5 25 6103.0 26 7112.0 27 8263.5 28 9384.5 29 10668.5 30 11946.5 31 13439.5 32 15857.5 33 18214.5 34 19963.0 35 21445.5 36 23778.5 37 25934.0 38 25646.5 39 24950.5 40 24233.5 41 23224.0 42 22768.5 43 23044.5 44 24025.5 45 24777.0 46 25661.0 47 32246.5 48 55205.5 49 53922.0 50 32075.0 51 24006.5 52 17580.0 53 15252.0 54 14062.5 55 12785.5 56 11733.0 57 10665.0 58 9872.0 59 9128.5 60 8195.5 61 7482.5 62 6445.5 63 4405.5 64 2645.5 65 1943.0 66 1498.5 67 1175.5 68 952.5 69 727.5 70 556.5 71 459.5 72 379.5 73 356.0 74 302.0 75 229.0 76 184.0 77 136.0 78 101.5 79 70.0 80 58.5 81 49.5 82 42.0 83 35.5 84 22.5 85 13.5 86 10.0 87 6.5 88 5.5 89 5.0 90 5.5 91 3.5 92 1.0 93 1.5 94 1.5 95 0.5 96 0.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23040907356266183 2 0.14146665736549896 3 0.16742101872407128 4 0.1419795894081585 5 0.20363402093583424 6 0.1660873954131565 7 0.20476247142968523 8 0.21184093361838677 9 0.1275149058051597 10-11 0.17567922461088975 12-13 0.18070595862895317 14-15 0.14813477392007288 16-17 0.2518496329458303 18-19 0.1989663393476325 20-21 0.16824170999232652 22-23 0.2099430850605465 24-25 0.19773530244524962 26-27 0.2974492915382627 28-29 0.2704177728901054 30-31 0.2501569572050538 32-33 0.16285592354440145 34-35 0.3150428606014846 36-37 0.35197396767297096 38-39 0.33386746656708943 40-41 0.29837256921504984 42-43 0.329969183042877 44-45 0.33756057727423805 46-47 0.34299765692642914 48-49 0.36053993278538515 50-51 0.352948538554024 52-53 0.3526920725326943 54-55 0.34771663171889683 56-57 0.32837909371063245 58-59 0.30509197897388973 60-61 0.27426476321005183 62-63 0.33576531512492974 64-65 0.3693623639191291 66-67 0.37377357948600104 68-69 0.3788516067083304 70-71 0.37844126107420284 72-73 0.36951624353192697 74-75 0.3643869231053316 76-77 0.3488450822127478 78-79 0.32668641796985604 80-81 0.3352523830822702 82-83 0.34525455791413107 84-85 0.3450493850970673 86-87 0.340997221960057 88-89 0.34930672105114136 90-91 0.36920848430633124 92-93 0.3676696881783526 94-95 0.3763382396992987 96-97 0.3773128105803518 98-99 0.3747994435713201 100-101 0.3484347365786202 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 974788.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.81836154766615 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4218080410662 30.377762676512482 2 13.487991649818603 11.011154394283956 3 4.876348707471959 5.971336937222549 4 2.2510608697697254 3.6753846579225793 5 1.274533762763371 2.6012189966591315 6 0.8059324661761997 1.9738105672429407 7 0.5428558659482916 1.5510940903184132 8 0.37216935083044184 1.2153074495325744 9 0.26896621836456874 0.9880884310782141 >10 1.4924906012018149 11.188587593787545 >50 0.12057962245437448 3.432119130910772 >100 0.06937065721168684 5.141355627950758 >500 0.006810937252583417 2.0504877827231405 >1k 0.006306423382021683 5.409379113455868 >5k 0.001513541611685204 3.7965017091887563 >10k+ 0.0012612846764043365 9.616410841210316 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26488 2.7173087891931376 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22058 2.262850999396792 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18264 1.873638165426739 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16191 1.660976540540097 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10392 1.0660779574635717 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7943 0.8148438429689326 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6957 0.713693644156473 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5826 0.5976684161068868 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5497 0.5639174876998897 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5465 0.5606347226268686 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5183 0.5317053554208709 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4517 0.46338280733862136 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4029 0.413320639975051 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3961 0.4063447641948813 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3806 0.3904438708724358 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3565 0.3657205464162464 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2706 0.2775988214873388 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2457 0.2520548057628941 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2108 0.21625214918525876 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2096 0.21502111228287588 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1900 0.1949141762106222 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1886 0.19347796649117552 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1865 0.19132365191200548 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1834 0.18814347324751637 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1602 0.1643434264681141 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1570 0.1610606613950931 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1546 0.15859858759032733 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1502 0.15408478561492345 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1461 0.1498787428651153 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1358 0.13931234278632892 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1318 0.1352088864450527 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1192 0.12228299897003246 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1126 0.11551229600692663 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1087 0.11151142607418228 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1030 0.1056640007878636 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1013 0.10392003184282121 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 982 0.1007398531783321 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0258640853190642E-4 0.0 12-13 0.0 0.0 0.0 1.5387961279785962E-4 0.0 14-15 0.0 0.0 0.0 3.0775922559571925E-4 0.0 16-17 0.0 0.0 0.0 4.6163883839357895E-4 0.0 18-19 0.0 0.0 0.0 6.668116554573917E-4 0.0 20-21 0.0 0.0 0.0 0.0013849165151807368 0.0 22-23 0.0 0.0 0.0 0.0035905242986167245 0.0 24-25 0.0 0.0 0.0 0.005129320426595321 0.0 26-27 0.0 0.0 0.0 0.0074375146185632155 0.0 28-29 0.0 0.0 0.0 0.01261812824942449 0.0 30-31 0.0 0.0 0.0 0.025544015724444702 0.0 32-33 0.0 0.0 0.0 0.04580483140949622 0.0 34-35 0.0 0.0 0.0 0.07442643938989811 0.0 36-37 0.0 0.0 0.0 0.11525582998559687 0.0 38-39 0.0 0.0 0.0 0.17367878964451758 0.0 40-41 0.0 0.0 0.0 0.24318108142488418 0.0 42-43 0.0 0.0 0.0 0.3116062159156658 0.0 44-45 0.0 0.0 0.0 0.39018740485110603 0.0 46-47 0.0 1.0258640853190642E-4 0.0 0.4862082832369705 0.0 48-49 0.0 1.0258640853190642E-4 0.0 0.596386086000238 0.0 50-51 0.0 1.0258640853190642E-4 0.0 0.7169251160252281 0.0 52-53 0.0 1.0258640853190642E-4 0.0 0.8377206120715479 0.0 54-55 0.0 1.0258640853190642E-4 0.0 0.9684156965411966 0.0 56-57 0.0 1.0258640853190642E-4 0.0 1.1279375618083112 0.0 58-59 0.0 1.0258640853190642E-4 0.0 1.307976708781807 0.0 60-61 0.0 1.0258640853190642E-4 0.0 1.5232029938817466 0.0 62-63 0.0 1.0258640853190642E-4 0.0 1.7425840285272285 0.0 64-65 0.0 1.0258640853190642E-4 0.0 1.9374469115335846 0.0 66-67 0.0 1.0258640853190642E-4 0.0 2.1660094297426724 0.0 68-69 0.0 1.0258640853190642E-4 0.0 2.438325051190618 0.0 70-71 0.0 1.0258640853190642E-4 0.0 2.7470075544631243 0.0 72-73 0.0 1.0258640853190642E-4 0.0 3.0596396344641095 1.0258640853190642E-4 74-75 0.0 1.0258640853190642E-4 0.0 3.3742721494314663 1.0258640853190642E-4 76-77 0.0 1.0258640853190642E-4 0.0 3.684442155627685 1.0258640853190642E-4 78-79 0.0 1.0258640853190642E-4 0.0 4.060677808918452 1.0258640853190642E-4 80-81 0.0 1.0258640853190642E-4 0.0 4.485077781014949 1.0258640853190642E-4 82-83 0.0 1.0258640853190642E-4 0.0 4.916966560934275 1.0258640853190642E-4 84-85 0.0 1.0258640853190642E-4 0.0 5.355779923429505 1.0258640853190642E-4 86-87 0.0 1.0258640853190642E-4 0.0 5.807108827765627 1.0258640853190642E-4 88-89 0.0 1.0258640853190642E-4 0.0 6.26464420981793 1.0258640853190642E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATAGTC 130 0.0 47.4537 7 AGATAGT 170 0.0 39.0755 6 CGACCGT 70 1.0913936E-11 37.297516 18-19 TCGACCG 80 1.8189894E-12 35.602173 18-19 CGATCCG 55 7.004019E-8 34.55264 70-71 TACCTGG 610 0.0 30.336132 2 GTAAGCG 65 3.0304363E-7 29.23986 94-95 CATGGGG 2105 0.0 27.27462 4 TAGTCAA 180 6.7739165E-9 26.360458 9 GTACATG 13825 0.0 25.681377 1 TACATGG 13655 0.0 25.33152 2 ATGGGAG 2675 0.0 25.010406 5 GTTCGAC 95 1.1999873E-8 24.984623 16-17 GGATATG 405 0.0 24.644901 1 ACATGGG 13550 0.0 24.232166 3 CATGGGT 650 0.0 24.089403 4 CGCTCCG 100 2.0334483E-8 23.77452 34-35 CTACACG 140 1.7940207E-5 23.724411 4 GATTGGC 325 0.0 23.35942 9 GATATGA 375 0.0 22.775436 2 >>END_MODULE