##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938698_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 974788 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.557080103571238 31.0 31.0 34.0 30.0 34.0 2 31.708913117518886 31.0 31.0 34.0 30.0 34.0 3 31.705880663282684 31.0 31.0 34.0 30.0 34.0 4 35.493195443522076 37.0 35.0 37.0 33.0 37.0 5 35.43139533929429 37.0 35.0 37.0 33.0 37.0 6 35.5466429623672 37.0 35.0 37.0 33.0 37.0 7 35.46192505447338 37.0 35.0 37.0 33.0 37.0 8 35.49057641251226 37.0 35.0 37.0 33.0 37.0 9 36.97813370702142 39.0 37.0 39.0 33.0 39.0 10-11 36.87668241709993 39.0 37.0 39.0 32.0 39.0 12-13 36.75660964230171 39.0 37.0 39.0 32.0 39.0 14-15 37.58596792328178 39.0 36.0 41.0 32.0 41.0 16-17 37.53549746201225 39.0 36.5 40.5 32.0 41.0 18-19 37.8005627890372 39.5 37.0 41.0 33.0 41.0 20-21 37.829513699389 40.0 37.0 41.0 33.0 41.0 22-23 37.654082220954706 39.5 36.0 41.0 32.0 41.0 24-25 37.466022355630145 39.0 36.0 41.0 32.0 41.0 26-27 37.11910076857737 39.0 35.5 41.0 31.5 41.0 28-29 36.81540447769156 39.0 35.0 40.5 31.0 41.0 30-31 36.34680104802275 38.0 35.0 40.0 30.5 41.0 32-33 35.829184909949646 38.0 35.0 40.0 30.0 41.0 34-35 35.52316247225038 38.0 35.0 40.0 30.0 41.0 36-37 35.23084660459505 37.0 34.5 40.0 28.0 41.0 38-39 34.97157176739968 37.0 34.0 40.0 27.0 41.0 40-41 34.68311520043332 37.0 33.5 40.0 25.5 41.0 42-43 34.31858516928809 37.0 33.0 40.0 24.0 41.0 44-45 33.81407854836128 36.0 33.0 40.0 23.0 41.0 46-47 33.1613673947566 36.0 33.0 40.0 20.0 41.0 48-49 32.60782806107584 36.0 32.5 40.0 16.5 41.0 50-51 32.31383285391286 35.0 32.0 39.5 13.5 41.0 52-53 32.066271332843655 35.0 31.0 39.0 10.5 41.0 54-55 31.78039789164413 35.0 31.0 39.0 8.0 40.0 56-57 31.477570507638582 35.0 31.0 39.0 6.5 40.0 58-59 31.154850593154613 35.0 30.5 39.0 2.0 40.0 60-61 30.678327492747144 35.0 29.5 38.0 2.0 40.0 62-63 30.27120922703193 35.0 28.5 38.0 2.0 40.0 64-65 29.813919026496016 34.5 28.0 37.5 2.0 40.0 66-67 29.335865336873248 34.0 28.0 37.0 2.0 39.0 68-69 29.012072881488077 34.0 27.5 36.0 2.0 39.0 70-71 28.542134289712223 34.0 26.0 36.0 2.0 39.0 72-73 27.986390374112116 33.0 26.0 35.0 2.0 38.0 74-75 27.432442746525396 33.0 25.5 35.0 2.0 37.5 76-77 25.707607192538276 30.5 23.0 34.0 2.0 35.5 78-79 26.6945279383825 32.5 24.5 35.0 2.0 36.0 80-81 26.707032708650495 33.0 25.0 35.0 2.0 36.0 82-83 26.28814880774076 33.0 24.0 35.0 2.0 36.0 84-85 25.760695146021494 32.0 21.5 35.0 2.0 35.0 86-87 25.008867056221455 31.5 19.0 35.0 2.0 35.0 88-89 24.179926301924112 31.0 11.5 34.0 2.0 35.0 90-91 23.42046065400887 30.5 4.5 34.0 2.0 35.0 92-93 22.931218890671612 30.0 2.0 34.0 2.0 35.0 94-95 22.35002585177495 30.0 2.0 34.0 2.0 35.0 96-97 21.390606470329956 29.0 2.0 34.0 2.0 35.0 98-99 20.244587028153816 29.0 2.0 34.0 2.0 35.0 100-101 16.78736607344366 21.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.030596997660413905 1101 2 0.3088073952589845 1101 3 0.05887065345943654 1101 4 0.12749342347742498 1101 5 0.03642982982019305 1101 6 0.11257813765733715 1101 7 0.18918426028032798 1101 8 0.2884057356960028 1101 9 0.2532580437903249 1101 10-11 0.34964053512846505 1101 12-13 0.2333486360422441 1101 14-15 0.43879799365868877 1101 16-17 0.4224325092115251 1101 18-19 0.4000186675059254 1101 20-21 0.32320763517874695 1101 22-23 0.36796456519059717 1101 24-25 0.3384132243988205 1101 26-27 0.30565370459414254 1101 28-29 0.28544038265057736 1101 30-31 0.3519173446418691 1101 32-33 0.38156355794020413 1101 34-35 0.5687599091299731 1101 36-37 0.6661189419364106 1101 38-39 0.8820594245836446 1101 40-41 0.9352475250331835 1101 42-43 0.8809587342257643 1101 44-45 0.7927304582066412 1101 46-47 0.9560893571861726 1101 48-49 0.8673981606327743 1101 50-51 0.7289256070658325 1101 52-53 0.44971446767373635 1101 54-55 0.4682738554110095 1101 56-57 0.25456778577913397 1101 58-59 0.4973476310338505 1101 60-61 0.6010099336848 1101 62-63 0.23992000223026366 1101 64-65 0.38427934168040423 1101 66-67 0.1661477661722408 1101 68-69 0.18159207254590015 1101 70-71 0.5652598960523427 1101 72-73 0.35645683302415065 1101 74-75 0.15906641245029718 1101 76-77 0.27156578347148397 1101 78-79 0.06944031054402444 1101 80-81 0.22743494405351683 1101 82-83 0.18013909437115672 1101 84-85 0.2910331980138139 1101 86-87 0.17698026695540747 1101 88-89 0.4875553742535885 1101 90-91 0.6185831439212528 1101 92-93 0.5516352620393796 1101 94-95 0.6036428837844916 1101 96-97 0.2672688237340921 1101 98-99 0.3495943747310015 1101 100-101 0.47517498957427406 1102 1 0.2261536070440009 1102 2 0.20069216669121204 1102 3 0.24550359762913487 1102 4 0.2579441607806814 1102 5 -0.03138588576667445 1102 6 -0.10248369946601343 1102 7 0.24131487473734126 1102 8 0.21295729423238896 1102 9 0.16837407778380964 1102 10-11 0.2756476936317185 1102 12-13 0.3686385140240134 1102 14-15 0.55656515788273 1102 16-17 0.5492814128540076 1102 18-19 0.2524159380913247 1102 20-21 0.28499225531137995 1102 22-23 0.26467718628673254 1102 24-25 0.5157790683268928 1102 26-27 0.36476216608049583 1102 28-29 0.46072491621875855 1102 30-31 0.5111375871728612 1102 32-33 0.6212313386536437 1102 34-35 0.7690935586117149 1102 36-37 0.7727108244496392 1102 38-39 0.7104905757402165 1102 40-41 0.8131690750636622 1102 42-43 0.6687535237988769 1102 44-45 1.0513135111464393 1102 46-47 1.1371821768536137 1102 48-49 1.1992297890229011 1102 50-51 1.1372077544662673 1102 52-53 1.0232735460258482 1102 54-55 1.0839644138645887 1102 56-57 1.0204664752687016 1102 58-59 0.9743929983305541 1102 60-61 0.8796702924379716 1102 62-63 1.073288149525311 1102 64-65 1.2412438276757811 1102 66-67 1.2665841638869395 1102 68-69 1.1970325350258584 1102 70-71 1.557739139403818 1102 72-73 1.6583700954499072 1102 74-75 1.3355477935672049 1102 76-77 1.1428021119346283 1102 78-79 1.4605121289242895 1102 80-81 1.7695955926093951 1102 82-83 1.6616143030667416 1102 84-85 1.873116992396909 1102 86-87 1.7518077026154408 1102 88-89 1.831890860407043 1102 90-91 1.836456443682632 1102 92-93 1.773533808509434 1102 94-95 1.8811929594342907 1102 96-97 2.380677656318454 1102 98-99 2.5508143688596725 1102 100-101 2.8866242665729693 1103 1 0.25949021509325476 1103 2 0.2947256342879001 1103 3 0.29649092447852965 1103 4 0.0854403129894763 1103 5 0.16474503737574508 1103 6 0.224406630297878 1103 7 0.35982684382402397 1103 8 0.19380839289957663 1103 9 0.3600350136976189 1103 10-11 0.3942128768520803 1103 12-13 0.3167547336919583 1103 14-15 0.24547533242509445 1103 16-17 0.24334926496783993 1103 18-19 0.20943119826750234 1103 20-21 0.21345199267916115 1103 22-23 0.29109186356347294 1103 24-25 0.3352153259815367 1103 26-27 0.44000695109539123 1103 28-29 0.3140544836287873 1103 30-31 0.632924272177938 1103 32-33 0.6919938562552446 1103 34-35 0.5706662409315229 1103 36-37 0.8137143611428357 1103 38-39 0.8385892756779967 1103 40-41 0.988456431385508 1103 42-43 1.1165136181107016 1103 44-45 1.0139162553583816 1103 46-47 0.746418513101446 1103 48-49 0.707146414755627 1103 50-51 0.6876015390554571 1103 52-53 0.6507827970983229 1103 54-55 0.5277498217304561 1103 56-57 0.48982741445722766 1103 58-59 0.5473782293617653 1103 60-61 0.7561070740471685 1103 62-63 0.8051830638914907 1103 64-65 0.8075199844691809 1103 66-67 0.7460639752633007 1103 68-69 0.7011586770830327 1103 70-71 0.5985052591929296 1103 72-73 0.8289132070849767 1103 74-75 0.9149883083525481 1103 76-77 0.8899080817417477 1103 78-79 0.9082804100259025 1103 80-81 0.9039653735308981 1103 82-83 1.0041987088695379 1103 84-85 1.101495680545714 1103 86-87 1.1011052760509479 1103 88-89 1.044956158242453 1103 90-91 1.039398775935311 1103 92-93 1.1727789864902434 1103 94-95 1.4207105937795106 1103 96-97 1.1577268098043874 1103 98-99 1.5350015843687252 1103 100-101 1.6049454834574028 1104 1 0.16763707243104875 1104 2 0.21566073418740217 1104 3 0.09980185303217581 1104 4 0.02332190879987195 1104 5 -0.06885752211678664 1104 6 0.014882293912698685 1104 7 0.11379562465364756 1104 8 0.027120520062837272 1104 9 0.24006064550318484 1104 10-11 0.008939178957021454 1104 12-13 0.14268065961788068 1104 14-15 0.04557122119411616 1104 16-17 0.1701607789961841 1104 18-19 0.28242467967522344 1104 20-21 0.19498648958582265 1104 22-23 0.07072840824856286 1104 24-25 0.018623802917517196 1104 26-27 0.14547860397804868 1104 28-29 0.0993673668381092 1104 30-31 0.030622019713881343 1104 32-33 -0.04313554748257786 1104 34-35 -0.11216690339029611 1104 36-37 0.18894878152288896 1104 38-39 0.19253685033755374 1104 40-41 0.31547908510718514 1104 42-43 0.1590578053150793 1104 44-45 0.24962099171036556 1104 46-47 0.4467439817117054 1104 48-49 0.6760683115092334 1104 50-51 0.4043161501066308 1104 52-53 0.5112733972329337 1104 54-55 0.5868463466845846 1104 56-57 0.66719582095185 1104 58-59 0.8782820732105954 1104 60-61 0.9618783793330365 1104 62-63 1.04161664708883 1104 64-65 0.7721453267644236 1104 66-67 0.7577932923324262 1104 68-69 0.7437070133206909 1104 70-71 0.8639447340175153 1104 72-73 0.9214071448863557 1104 74-75 1.1033889943260498 1104 76-77 0.9659589396696298 1104 78-79 1.1071680081044413 1104 80-81 1.290212711234549 1104 82-83 1.2677761897609372 1104 84-85 1.492129007424996 1104 86-87 1.8017165669534982 1104 88-89 1.7289233716595085 1104 90-91 1.6115075912480101 1104 92-93 1.5167190928898933 1104 94-95 1.6981218736179748 1104 96-97 1.2646842776032798 1104 98-99 1.5203518088775247 1104 100-101 1.5633242256549167 1105 1 0.006836814038024386 1105 2 0.0759499063797513 1105 3 0.11567402448354613 1105 4 0.1515323471180423 1105 5 -0.022097884626994357 1105 6 -0.0063477328765770835 1105 7 0.06144443813568046 1105 8 0.11615401615843979 1105 9 0.15945722179561983 1105 10-11 0.15609115585364464 1105 12-13 0.097377332807703 1105 14-15 0.05131476611840213 1105 16-17 -0.01570835253726699 1105 18-19 0.09159668203115956 1105 20-21 0.06193308202782788 1105 22-23 -0.03766271519109665 1105 24-25 -0.011516033306904205 1105 26-27 0.03348137790315775 1105 28-29 0.2493493173254535 1105 30-31 0.22587879778087228 1105 32-33 0.4683410926481386 1105 34-35 0.23384320433680017 1105 36-37 0.42590848361093236 1105 38-39 0.5665968507175378 1105 40-41 0.5125921130554332 1105 42-43 0.36642580637905553 1105 44-45 0.6601790066439648 1105 46-47 0.5124736570104744 1105 48-49 0.5368144213642694 1105 50-51 0.46462432178599755 1105 52-53 0.48001639117209294 1105 54-55 0.3792750511095697 1105 56-57 0.5032653970732959 1105 58-59 0.5997163444852696 1105 60-61 0.6318952888764784 1105 62-63 0.5315858679198655 1105 64-65 0.5343125982480927 1105 66-67 0.5615798900942863 1105 68-69 0.37858437163201586 1105 70-71 0.21273066679733077 1105 72-73 0.32397112565887554 1105 74-75 0.5151173776597027 1105 76-77 0.5438178874780384 1105 78-79 0.08619162446679951 1105 80-81 0.05658076929698197 1105 82-83 0.3834222673168419 1105 84-85 0.6906185482073965 1105 86-87 0.8323874388399553 1105 88-89 0.71019367736125 1105 90-91 0.5149341487309584 1105 92-93 0.752397279578858 1105 94-95 0.7648670714976298 1105 96-97 1.1287619475061916 1105 98-99 1.2088181702857703 1105 100-101 1.6536791360723377 1106 1 0.24028551240684948 1106 2 0.028852517771856157 1106 3 0.11936241500580635 1106 4 0.1425838142477147 1106 5 0.1454078884639145 1106 6 0.20006470486791983 1106 7 0.07193665583492503 1106 8 0.1749756668462794 1106 9 0.29234090248223765 1106 10-11 0.17843684060126463 1106 12-13 0.16800212351501642 1106 14-15 0.2746177290254437 1106 16-17 0.2182929753220364 1106 18-19 0.043181596310681414 1106 20-21 0.041223993547106375 1106 22-23 0.0510401249954171 1106 24-25 0.09803090729365493 1106 26-27 0.1458171133842896 1106 28-29 0.08185370642306111 1106 30-31 0.04768385409875009 1106 32-33 0.16783791914183865 1106 34-35 0.1335457909525175 1106 36-37 0.1073814589642339 1106 38-39 0.31277635174440377 1106 40-41 0.13632982406601712 1106 42-43 0.17719230638582673 1106 44-45 0.4463588173756179 1106 46-47 0.445858575548435 1106 48-49 0.3186830445909692 1106 50-51 0.42299370669292813 1106 52-53 0.4725186316927079 1106 54-55 0.5723038865614747 1106 56-57 0.5349380352355055 1106 58-59 0.7294579732614856 1106 60-61 0.7584150199752919 1106 62-63 0.8752865692076561 1106 64-65 0.7528901434891893 1106 66-67 0.4868892561479292 1106 68-69 0.5304340835097747 1106 70-71 0.552399882721577 1106 72-73 0.8199589968449068 1106 74-75 1.0118882212761982 1106 76-77 0.9867755876102429 1106 78-79 0.9774920168240584 1106 80-81 1.033392106815203 1106 82-83 1.1846426009850433 1106 84-85 1.1809605977950035 1106 86-87 1.2028747012625054 1106 88-89 1.187665557133851 1106 90-91 1.2391333198835035 1106 92-93 1.3084969154821593 1106 94-95 1.4968432203572384 1106 96-97 1.5130349445388305 1106 98-99 1.6504383328420715 1106 100-101 1.8281624613727026 1107 1 -0.14316022089051472 1107 2 -0.11961434671874827 1107 3 -0.19908017109256448 1107 4 -0.09975350553767726 1107 5 -0.11942141345737411 1107 6 -0.16641612719154608 1107 7 -0.10490595424210625 1107 8 -0.15271668695883278 1107 9 -0.05174086474492867 1107 10-11 -0.12444809180889393 1107 12-13 -0.07701631007908816 1107 14-15 0.06966163994473362 1107 16-17 -0.03871928376737088 1107 18-19 -0.08235417685120439 1107 20-21 -0.053905341344837154 1107 22-23 0.037012609484222025 1107 24-25 -0.12128989719035133 1107 26-27 0.022947467369242247 1107 28-29 -0.05325105034341249 1107 30-31 -0.05547683288983052 1107 32-33 -0.3051096575149401 1107 34-35 -0.1352864483544991 1107 36-37 -0.24755215012367415 1107 38-39 -0.5514564810344282 1107 40-41 -0.8468971039680326 1107 42-43 -0.6640225124254258 1107 44-45 -0.44689268878389754 1107 46-47 -0.5518634518471828 1107 48-49 -0.5477573234814486 1107 50-51 -0.4258103576897767 1107 52-53 -0.39248751283865957 1107 54-55 -0.19516306784909787 1107 56-57 -0.4107682861384703 1107 58-59 -0.42926426394739536 1107 60-61 -0.30550908756899275 1107 62-63 -0.46722909185399786 1107 64-65 -0.2928296658804683 1107 66-67 -0.4125607333614063 1107 68-69 -0.36263308670872973 1107 70-71 0.024849902204234553 1107 72-73 0.1866616315000691 1107 74-75 0.04069408405832675 1107 76-77 -0.013981238814242403 1107 78-79 0.04334041175257397 1107 80-81 0.044900364465892295 1107 82-83 -0.15550516750100485 1107 84-85 0.10200936439273178 1107 86-87 0.17113894608461422 1107 88-89 -0.18997434335471297 1107 90-91 0.0453557818923187 1107 92-93 0.3660277130723024 1107 94-95 0.13808951115843016 1107 96-97 0.3540144727587169 1107 98-99 0.7017286844291597 1107 100-101 0.9029339147791688 1108 1 0.08594766834668377 1108 2 -0.06666894441989868 1108 3 -0.083040463673548 1108 4 -0.005346069104376738 1108 5 0.06104706442489771 1108 6 -0.1285852320330676 1108 7 -0.10135753905576195 1108 8 0.04317760074579979 1108 9 0.06301009310947592 1108 10-11 0.07880861317195098 1108 12-13 0.20173238375581093 1108 14-15 0.08176870194403563 1108 16-17 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-1.675281968157197 2316 92-93 -1.2261093437706378 2316 94-95 -1.087836414767498 2316 96-97 -1.3440049370253995 2316 98-99 -0.9506975385852989 2316 100-101 -0.952146299124573 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 53.0 5 164.0 6 522.0 7 827.0 8 1268.0 9 2024.0 10 3119.0 11 4335.0 12 5828.0 13 7555.0 14 8928.0 15 11791.0 16 15283.0 17 19843.0 18 17110.0 19 11392.0 20 10789.0 21 11185.0 22 12181.0 23 13517.0 24 15349.0 25 17761.0 26 20214.0 27 23524.0 28 27847.0 29 32908.0 30 38197.0 31 44596.0 32 53397.0 33 66326.0 34 90896.0 35 134216.0 36 108371.0 37 99742.0 38 42064.0 39 1650.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.555148963464276 22.65916024570954 16.563602424202553 15.222088366623632 2 15.930951140145345 27.31055367936413 38.01288074945526 18.745614431035264 3 21.27898578973069 32.72547466731228 30.709036221209125 15.286503321747908 4 11.889867335256486 21.455844758039696 37.28585087218965 29.368437034514173 5 9.96621153650806 43.33952737193885 32.451289923075585 14.242971168477508 6 23.816646507672214 40.617244814316685 18.177360166852868 17.388748511158234 7 22.446829464457913 39.95320008042774 21.407423973212637 16.192546481901708 8 25.326942883991187 33.83853719988346 20.242247545107244 20.592272371018108 9 26.69616367866654 19.695974919674843 21.821873063681537 31.785988337977077 10-11 23.298963466928193 30.75355872251197 25.500313914410107 20.44716389614973 12-13 27.546758885008842 29.576123218587014 26.99443366147306 15.882684234931082 14-15 22.895747588193537 29.360794347078546 30.73119488545202 17.012263179275905 16-17 16.480193606432756 32.94520400385325 31.295414978646047 19.279187411067944 18-19 16.18326241192957 30.05761252703152 37.31760136563027 16.44152369540864 20-21 18.43026381120818 27.768499407050555 37.36504757957628 16.436189202164982 22-23 17.411119135647958 28.47044690743013 37.180443337423114 16.937990619498805 24-25 17.499098518698236 29.09119913170552 37.75793521542454 15.651767134171706 26-27 17.485018329880397 28.147843424299673 38.903407916395025 15.463730329424907 28-29 16.05472164203909 29.745236913051865 38.74473218792189 15.45530925698716 30-31 16.342579104379617 29.867571205226163 37.75682507375963 16.03302461663459 32-33 16.120274357090977 30.221289141844178 37.69445253737223 15.963983963692618 34-35 17.02298138908111 29.277636170667886 37.74670457827271 15.952677861978296 36-37 17.262841013675946 29.19905071765622 37.940410268091206 15.59769800057663 38-39 16.93617021276596 29.58095751093327 38.162953842996934 15.319918433303837 40-41 16.392919560465717 30.270176826318707 37.517075530399445 15.819828082816132 42-43 15.812392852416163 30.99690471085157 36.51056163544532 16.68014080128695 44-45 16.755937419851243 30.180456527314696 35.84221595280842 17.221390100025648 46-47 17.16105020296825 31.080404561835813 34.96388426962719 16.79466096556875 48-49 17.252529563293198 30.93217600405428 34.856320394594434 16.95897403805809 50-51 16.78272609018576 31.49351448725261 35.029616696143165 16.694142726418463 52-53 16.905573314402723 32.31333377103534 33.86038810490076 16.920704809661178 54-55 15.998555583367871 31.52136669716902 34.29304628288407 18.18703143657903 56-57 17.33015107029498 31.562981519894784 33.36021124809957 17.746656161710668 58-59 18.043176744796796 31.053289754448166 33.37154692731929 17.531986573435756 60-61 17.058222780989666 31.432823004941195 34.54413860988987 16.964815604179265 62-63 17.565628810148738 31.07675925322752 34.44592454821927 16.911687388404477 64-65 17.101657722685637 32.2138738226722 33.96315647470021 16.721311979941955 66-67 17.172305258525085 31.46815410452623 33.55363116971585 17.805909467232834 68-69 16.97807673782926 31.859950871293357 33.46189139878322 17.700080992094165 70-71 17.563272797031555 31.895326876538157 33.079893351060285 17.461506975370003 72-73 17.39942428507532 30.92508319757732 33.51980122857483 18.155691288772534 74-75 16.69135592229245 32.80355730454881 32.87618885433989 17.628897918818858 76-77 17.147848256651166 31.265786926654233 33.55188273052633 18.034482086168264 78-79 16.94208424408763 31.163101714135454 33.95062616069814 17.944187881078772 80-81 16.844587337232337 31.841217342597655 33.452710819580645 17.861484500589363 82-83 16.70858208591575 31.518326433489385 33.485025603199894 18.288065877394963 84-85 16.408285699044306 31.401186719573897 33.251435183855364 18.939092397526437 86-87 16.796145813439132 31.469439430250684 32.836979490749584 18.8974352655606 88-89 17.012466708105727 30.905683600423174 32.90319725678907 19.17865243468202 90-91 17.05178167270878 30.494200755319902 33.20689763443937 19.247119937531945 92-93 16.851049205722287 31.55675354052432 33.23697020368594 18.35522705006745 94-95 16.577416125008206 31.373155488805725 32.92385800341409 19.12557038277198 96-97 16.035921712087724 31.02099793134189 33.125048408036015 19.81803194853437 98-99 16.886902588049914 31.04039032076718 32.197257249781494 19.875449841401412 100-101 17.188187657279563 30.06364532605919 33.031965259746585 19.716201756914657 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22658.0 1 14218.0 2 8767.0 3 10658.5 4 7511.5 5 6134.5 6 9776.5 7 18792.0 8 24651.0 9 22724.0 10 15822.5 11 8934.5 12 7031.0 13 6507.5 14 5937.5 15 5359.5 16 4733.5 17 4340.5 18 4217.5 19 4262.5 20 4320.5 21 4511.5 22 4835.0 23 5256.5 24 5759.5 25 6477.5 26 7454.5 27 8531.0 28 9676.5 29 10958.0 30 12458.0 31 13967.0 32 16102.0 33 18306.5 34 20077.5 35 21571.5 36 23916.0 37 25981.5 38 26067.5 39 25587.5 40 24750.5 41 23919.5 42 23428.5 43 23510.5 44 24300.5 45 25048.5 46 25907.5 47 32995.5 48 52295.5 49 51886.5 50 34226.5 51 24776.0 52 17519.0 53 14770.0 54 13468.0 55 12248.5 56 11295.5 57 10227.5 58 9489.0 59 8781.0 60 7772.5 61 6923.0 62 5893.5 63 4211.5 64 2690.0 65 1901.5 66 1469.5 67 1188.0 68 936.5 69 728.5 70 535.5 71 419.5 72 348.5 73 327.0 74 286.5 75 227.5 76 188.0 77 123.0 78 83.0 79 53.0 80 34.0 81 25.0 82 20.0 83 18.0 84 9.0 85 4.0 86 2.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19819694128364324 2 0.0 3 0.0 4 0.0 5 0.020004349663721752 6 0.003795697115680538 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0027698330303614735 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.5905242986167247E-4 26-27 0.0013849165151807368 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006616823350307965 36-37 0.01610606613950931 38-39 0.012874594270754255 40-41 0.007642687435627029 42-43 0.008309499091084421 44-45 0.003898283524212444 46-47 5.129320426595322E-4 48-49 0.0015387961279785963 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0010258640853190643 58-59 4.103456341276257E-4 60-61 0.002410780600499801 62-63 6.668116554573917E-4 64-65 0.0017439689450424094 66-67 0.004821561200999602 68-69 1.5387961279785965E-4 70-71 1.0258640853190642E-4 72-73 0.0 74-75 5.129320426595322E-4 76-77 0.04975440813797462 78-79 0.12679680094543636 80-81 9.232776767871577E-4 82-83 7.693980639892981E-4 84-85 0.0 86-87 0.09089155795926909 88-89 0.3159661382782718 90-91 0.34997353270659876 92-93 3.077592255957193E-4 94-95 4.103456341276257E-4 96-97 1.5387961279785965E-4 98-99 0.0 100-101 0.0011284504938509706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 974788.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.87022050565899 #Duplication Level Percentage of deduplicated Percentage of total 1 75.33574797303314 30.78988631616617 2 12.978065785639176 10.608328207920433 3 4.662116883555675 5.716252351622283 4 2.2250147513880485 3.637473740702943 5 1.2200912393819412 2.4932698995281353 6 0.7687254910609284 1.885078819678868 7 0.5090749183761003 1.4564202917552103 8 0.3520829314540349 1.1511765635844182 9 0.2627501399079357 0.9664790540337075 >10 1.4795410252771266 11.133408332584853 >50 0.12286758915460758 3.4934648892071736 >100 0.06758928774553495 5.042849116410642 >500 0.006281532317629715 1.760124500570015 >1k 0.007537838781155658 6.099642653725656 >5k 0.0012563064635259431 3.5882304725156042 >10k+ 0.0012563064635259431 10.177914789993894 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25440 2.6097982330516993 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22996 2.3590770505997205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21171 2.1718568550289907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18912 1.9401141581554142 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10593 1.0866978255784847 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9436 0.9680053509070691 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8329 0.8544421966622486 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6446 0.6612719893966689 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5414 0.5554028157917413 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5317 0.5454519341641465 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4728 0.48502853953885355 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4581 0.46994833748466336 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3232 0.33155927237512156 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2784 0.2856005613528275 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2584 0.26508327964644623 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2496 0.2560556756956384 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2460 0.2523625649884898 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2452 0.2515418737202345 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2317 0.23769270856842717 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2050 0.2103021374904082 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2016 0.20681419960032332 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1958 0.20086418790547278 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1911 0.19604262670447317 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1841 0.18886157810723972 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1781 0.18270639359532534 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1749 0.17942362852230434 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1701 0.17449948091277284 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1618 0.1659848090046246 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1577 0.16177876625481644 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1516 0.15552099533437014 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1279 0.13120801651230832 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1275 0.1307976708781807 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1271 0.13038732524405305 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1267 0.12997697960992544 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1263 0.12956663397579782 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1232 0.1263864553113087 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1224 0.12556576404305347 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1210 0.12412955432360678 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1015 0.10412520465988503 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1010 0.1036122726172255 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 991 0.10166313085511927 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0517281706381284E-4 0.0 12-13 0.0 0.0 0.0 2.5646602132976603E-4 0.0 14-15 0.0 0.0 0.0 4.103456341276257E-4 0.0 16-17 0.0 0.0 0.0 5.642252469254853E-4 0.0 18-19 0.0 0.0 0.0 7.693980639892981E-4 0.0 20-21 0.0 0.0 0.0 0.0014362097194466899 0.0 22-23 0.0 0.0 0.0 0.0036418175028826777 0.0 24-25 0.0 0.0 0.0 0.0052319068351272275 0.0 26-27 0.0 0.0 0.0 0.007591394231361076 0.0 28-29 0.0 0.0 0.0 0.012515541840892583 0.0 30-31 0.0 0.0 0.0 0.024825910864721357 0.0 32-33 0.0 0.0 0.0 0.04477896732417715 0.0 34-35 0.0 0.0 0.0 0.07293893646618546 0.0 36-37 0.0 0.0 0.0 0.11233211734243753 0.0 38-39 0.0 0.0 0.0 0.16967791971177323 0.0 40-41 0.0 0.0 0.0 0.23805176099828887 0.0 42-43 0.0 0.0 0.0 0.3053997381994854 0.0 44-45 0.0 0.0 0.0 0.3822882513941493 0.0 46-47 0.0 0.0 0.0 0.4774371453074925 0.0 48-49 0.0 0.0 0.0 0.5861274451470473 0.0 50-51 0.0 0.0 0.0 0.7040505217544738 0.0 52-53 0.0 0.0 0.0 0.8234611012856128 0.0 54-55 0.0 0.0 0.0 0.9509760070907726 0.0 56-57 0.0 0.0 0.0 1.10742028010193 0.0 58-59 0.0 0.0 0.0 1.2855102853133193 0.0 60-61 0.0 0.0 0.0 1.496530527663451 0.0 62-63 0.0 0.0 0.0 1.7106796554738057 0.0 64-65 0.0 0.0 0.0 1.902157186998609 0.0 66-67 0.0 0.0 0.0 2.125692971189633 0.0 68-69 0.0 0.0 0.0 2.3896990935464943 0.0 70-71 0.0 0.0 0.0 2.6867380394506295 0.0 72-73 0.0 0.0 0.0 2.990598981522136 0.0 74-75 0.0 0.0 0.0 3.2969732906026747 0.0 76-77 0.0 0.0 0.0 3.600064834610192 0.0 78-79 0.0 0.0 0.0 3.9650672761667156 0.0 80-81 0.0 0.0 0.0 4.3753103238858095 0.0 82-83 0.0 0.0 0.0 4.790631398827232 0.0 84-85 0.0 0.0 0.0 5.209542998067272 0.0 86-87 0.0 0.0 0.0 5.636097284742939 0.0 88-89 0.0 0.0 0.0 6.068755462726254 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACCGA 45 1.5252066E-5 52.72263 6 TCGACCG 35 2.067441E-4 33.89051 18-19 TATAACG 85 1.6413609E-5 33.501247 2 GTAAGCG 110 0.0 32.350864 94-95 GTACTAA 190 1.2732926E-11 30.033363 1 CGACCGT 40 4.5244177E-4 29.654196 18-19 CGTCACT 165 2.6157068E-9 28.819895 1 GTTCGAC 45 9.008628E-4 26.359287 16-17 TAACGCA 135 1.3512239E-5 24.608944 4 CATATAG 260 2.5465852E-11 23.730055 3 TAGGCCC 120 1.7203283E-4 23.730053 4 CCGATCG 165 2.4536057E-6 23.003878 9 GCCGATC 165 2.4536057E-6 23.003878 8 ATGGGAT 3110 0.0 22.585516 5 AGTACTT 14460 0.0 22.280184 12-13 GAGTACT 14135 0.0 22.25538 12-13 TACCTGG 585 0.0 21.09338 2 CATGGGG 1915 0.0 21.065844 4 GTACATG 12875 0.0 20.572367 1 GATTGGC 255 5.638867E-11 20.466686 9 >>END_MODULE