##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938697_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1572596 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.778320051685238 31.0 31.0 34.0 30.0 34.0 2 31.99764593067768 33.0 31.0 34.0 30.0 34.0 3 32.052414606167126 33.0 31.0 34.0 30.0 34.0 4 35.75124443913122 37.0 35.0 37.0 33.0 37.0 5 35.67448855268613 37.0 35.0 37.0 33.0 37.0 6 35.73009851226889 37.0 35.0 37.0 33.0 37.0 7 35.67034317777738 37.0 35.0 37.0 33.0 37.0 8 35.655385744336115 37.0 35.0 37.0 33.0 37.0 9 37.22346553088015 39.0 37.0 39.0 33.0 39.0 10-11 37.146094101727336 39.0 37.0 39.0 33.0 39.0 12-13 37.09477195668818 39.0 37.0 39.0 33.0 39.0 14-15 38.13661423531536 40.0 37.0 41.0 33.0 41.0 16-17 38.084203762441206 40.0 37.0 41.0 33.0 41.0 18-19 38.17279422051182 40.0 37.0 41.0 33.0 41.0 20-21 38.25565243711672 40.0 37.0 41.0 33.0 41.0 22-23 38.17017053330925 40.0 37.0 41.0 33.0 41.0 24-25 38.06371661889004 40.0 37.0 41.0 33.0 41.0 26-27 37.82933061002318 40.0 37.0 41.0 33.0 41.0 28-29 37.63830888543529 40.0 36.0 41.0 32.0 41.0 30-31 37.328948757341365 39.0 36.0 41.0 32.0 41.0 32-33 36.979572948169775 39.0 35.0 41.0 31.0 41.0 34-35 36.79749821314566 39.0 35.0 41.0 31.0 41.0 36-37 36.628253537462896 39.0 35.0 41.0 30.5 41.0 38-39 36.46067203528433 39.0 35.0 41.0 30.0 41.0 40-41 36.30210619892204 39.0 35.0 41.0 30.0 41.0 42-43 36.05219617753066 39.0 35.0 41.0 30.0 41.0 44-45 35.70738797504254 39.0 35.0 41.0 28.5 41.0 46-47 35.28885931288137 38.0 35.0 40.0 26.5 41.0 48-49 34.92529613454441 38.0 35.0 40.0 24.0 41.0 50-51 34.6895238828027 38.0 34.0 40.0 24.0 41.0 52-53 34.51746570638613 38.0 34.0 40.0 23.5 41.0 54-55 34.31360788149023 38.0 34.0 40.0 23.0 41.0 56-57 34.052257223088446 38.0 33.5 40.0 22.5 41.0 58-59 33.774906587578755 38.0 33.0 40.0 22.0 41.0 60-61 33.39582162233657 37.0 33.0 40.0 20.0 41.0 62-63 33.04257673299436 37.0 33.0 40.0 18.5 41.0 64-65 32.68221335931161 36.0 32.0 39.0 15.0 41.0 66-67 32.249228345996045 36.0 31.5 39.0 11.0 40.0 68-69 31.771005712846783 35.5 31.0 39.0 8.0 40.0 70-71 31.257495249892536 35.0 31.0 38.0 6.0 40.0 72-73 30.885441969838407 35.0 30.5 37.0 2.0 39.5 74-75 30.44281461990238 35.0 30.0 37.0 2.0 39.0 76-77 28.39253692620355 32.5 27.0 35.0 2.0 37.5 78-79 29.437540220120106 34.0 29.0 36.0 2.0 38.0 80-81 29.442738948846365 34.0 29.0 36.0 2.0 37.0 82-83 29.085598271902 34.0 29.0 35.0 2.0 37.0 84-85 28.638602667182163 34.0 29.0 35.0 2.0 36.5 86-87 28.101183965875535 34.0 28.5 35.0 2.0 36.0 88-89 27.505709349381533 33.5 26.5 35.0 2.0 35.5 90-91 27.012042826002357 33.0 25.5 35.0 2.0 35.0 92-93 26.70340252677738 33.0 25.0 35.0 2.0 35.0 94-95 26.259862354985007 33.0 25.0 35.0 2.0 35.0 96-97 25.654335887920354 32.5 23.0 35.0 2.0 35.0 98-99 24.87275593985995 32.0 10.5 34.0 2.0 35.0 100-101 21.735876855848545 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03750138496496902 1101 2 0.05591241393546298 1101 3 0.03785995274048304 1101 4 0.12237642136250315 1101 5 0.08336016428280857 1101 6 0.16046847426861177 1101 7 0.06779448350444994 1101 8 0.13413701034592407 1101 9 0.2285743073465767 1101 10-11 0.19510650517820238 1101 12-13 0.15368791486022815 1101 14-15 0.2413066490335254 1101 16-17 0.13582919319200926 1101 18-19 0.22564624021134705 1101 20-21 0.22336031645301802 1101 22-23 0.14934869661770023 1101 24-25 0.18683713362585053 1101 26-27 0.0733850025672993 1101 28-29 0.12853078534246976 1101 30-31 0.18113068405695287 1101 32-33 0.1008914603646005 1101 34-35 0.17031619986941848 1101 36-37 0.13261715213797487 1101 38-39 0.1748088608751175 1101 40-41 0.20682595633227407 1101 42-43 0.1446708007110118 1101 44-45 0.25088034488538113 1101 46-47 0.3653117448868528 1101 48-49 0.26655736246385686 1101 50-51 0.27900003895558 1101 52-53 0.3068793892364283 1101 54-55 0.12949333091297888 1101 56-57 0.058582142019325545 1101 58-59 0.21112962263421053 1101 60-61 0.1358330541617221 1101 62-63 0.2275928914500227 1101 64-65 0.2624583269486678 1101 66-67 0.2068998273536593 1101 68-69 0.19000969298850734 1101 70-71 0.11954765419767455 1101 72-73 0.25164224505690314 1101 74-75 0.2521798967065756 1101 76-77 0.19302436912577647 1101 78-79 0.3161299194642879 1101 80-81 0.321160772753192 1101 82-83 0.39812017609496664 1101 84-85 0.3606656097796481 1101 86-87 0.1514449664053963 1101 88-89 0.521264127644109 1101 90-91 0.6714897358081302 1101 92-93 0.5905635066700334 1101 94-95 0.45955815228180796 1101 96-97 0.548048876474251 1101 98-99 0.7718838160256354 1101 100-101 1.3137395488194556 1102 1 0.13436582406983177 1102 2 0.14082960315511528 1102 3 0.15539732509866866 1102 4 0.1397949893446082 1102 5 0.1201842568708571 1102 6 0.2493288025962883 1102 7 0.23520215906079045 1102 8 0.15191757939216188 1102 9 0.18089747144643553 1102 10-11 0.2618132997033271 1102 12-13 0.20734505693067007 1102 14-15 0.31449119749995447 1102 16-17 0.37327739343123767 1102 18-19 0.3202599154291761 1102 20-21 0.2694800213776105 1102 22-23 0.34281801925458666 1102 24-25 0.32304509554365524 1102 26-27 0.40348684877272234 1102 28-29 0.36228244451401537 1102 30-31 0.2490143869634096 1102 32-33 0.5026579769668444 1102 34-35 0.6061148657294808 1102 36-37 0.5843238211369979 1102 38-39 0.6474678599219317 1102 40-41 0.5270642858471817 1102 42-43 0.6936316470970354 1102 44-45 0.578251796039936 1102 46-47 0.6919966492805116 1102 48-49 0.6699012561861792 1102 50-51 0.6009681990588547 1102 52-53 0.7201692014574874 1102 54-55 0.6913888316527732 1102 56-57 0.6392079584451125 1102 58-59 0.46438155836809614 1102 60-61 0.5023449592996272 1102 62-63 0.6241506824498728 1102 64-65 0.5477121285672197 1102 66-67 0.5814229294193254 1102 68-69 0.5645631642709752 1102 70-71 0.6346555297049399 1102 72-73 0.6248131071296399 1102 74-75 0.6743205134689241 1102 76-77 0.6606724474022307 1102 78-79 0.6255772564079152 1102 80-81 0.8129515685319539 1102 82-83 0.8619014432282661 1102 84-85 0.9529364011528436 1102 86-87 0.8624199154458516 1102 88-89 1.052736961772304 1102 90-91 1.0501944765643465 1102 92-93 1.0756985848970864 1102 94-95 1.216676089313058 1102 96-97 1.3471781707827368 1102 98-99 1.5003411084034468 1102 100-101 1.346240279705274 1103 1 -0.013127974130412667 1103 2 0.01519963403459812 1103 3 0.032137232640941704 1103 4 -0.029014445824749657 1103 5 0.04881687297613979 1103 6 0.07950009154654936 1103 7 0.1361480615356072 1103 8 0.08616344174654955 1103 9 0.22560826095151043 1103 10-11 0.08778218407815785 1103 12-13 0.17537099906808606 1103 14-15 0.1652202930070743 1103 16-17 0.03422478988015598 1103 18-19 0.12951074765709336 1103 20-21 0.08338506486551012 1103 22-23 0.06299787445957605 1103 24-25 0.18989525596514767 1103 26-27 0.23216416361933767 1103 28-29 0.309522267116634 1103 30-31 0.23215476872198337 1103 32-33 0.26978476356106995 1103 34-35 0.1996816204683327 1103 36-37 0.14037254878912364 1103 38-39 0.40822718430828786 1103 40-41 0.3653765288444859 1103 42-43 0.3496923024456464 1103 44-45 0.41056585349287644 1103 46-47 0.4592131948027429 1103 48-49 0.4614527121482723 1103 50-51 0.39328049747776817 1103 52-53 0.37917021470927637 1103 54-55 0.23756106518020204 1103 56-57 0.5123912437664515 1103 58-59 0.37992864807039695 1103 60-61 0.560440070028406 1103 62-63 0.5888922911949308 1103 64-65 0.6053289266192721 1103 66-67 0.5058730204433104 1103 68-69 0.44826209957346563 1103 70-71 0.2966547321155417 1103 72-73 0.3894411015537429 1103 74-75 0.49781289480340263 1103 76-77 0.24398620244589608 1103 78-79 0.31791979575425344 1103 80-81 0.3457912880021716 1103 82-83 0.41472427115257204 1103 84-85 0.30275164233929885 1103 86-87 0.4078084149777794 1103 88-89 0.5498146761365028 1103 90-91 0.6454732774332648 1103 92-93 0.3980241001675502 1103 94-95 0.4980667141436328 1103 96-97 0.393878716926654 1103 98-99 0.6032205025246569 1103 100-101 0.9202401905487534 1104 1 0.09895002158736332 1104 2 0.041632711518293064 1104 3 0.15617294320001918 1104 4 0.11329756778389566 1104 5 0.12000098845872742 1104 6 0.1682668930539819 1104 7 -0.03379700723156276 1104 8 -0.030514670612134864 1104 9 -0.04868875920504223 1104 10-11 0.08876598781730394 1104 12-13 -0.027119672490705682 1104 14-15 0.15776684219159876 1104 16-17 0.2522476294956846 1104 18-19 0.04094556019445861 1104 20-21 -0.04361156128421584 1104 22-23 0.020639309644202797 1104 24-25 0.11262207277665226 1104 26-27 0.10719459783572205 1104 28-29 0.15614332088065197 1104 30-31 0.08936591868642552 1104 32-33 0.0833621449323445 1104 34-35 0.0665022984253838 1104 36-37 -0.11421927680279254 1104 38-39 0.03451765574946819 1104 40-41 0.009042005644509743 1104 42-43 0.21792023430212737 1104 44-45 0.13750844626972025 1104 46-47 0.19414330328202567 1104 48-49 0.2817749629779911 1104 50-51 0.14128356799698594 1104 52-53 0.08489318204739504 1104 54-55 0.15244899850884508 1104 56-57 0.3073665445293088 1104 58-59 0.034317888958121046 1104 60-61 0.22163727323514593 1104 62-63 0.08178888442312626 1104 64-65 0.2634867526010751 1104 66-67 -0.07371275874947258 1104 68-69 0.06963618118811965 1104 70-71 0.12465267431762683 1104 72-73 0.06429710018835166 1104 74-75 0.1832990293384107 1104 76-77 0.025673580557366904 1104 78-79 0.07561206922459363 1104 80-81 0.11806913440017652 1104 82-83 0.1933403211674154 1104 84-85 0.2061554617222363 1104 86-87 0.422152940822798 1104 88-89 0.757540639576618 1104 90-91 0.672902291898513 1104 92-93 0.7139692837960823 1104 94-95 0.6880162641216714 1104 96-97 0.7718411491726052 1104 98-99 0.7596491392416311 1104 100-101 1.2527508900463573 1105 1 -0.12293971941928561 1105 2 -0.05655153172654437 1105 3 -0.040962206913363275 1105 4 -0.06641476774494492 1105 5 -0.018799057751138548 1105 6 0.009262957944905281 1105 7 -0.06624448996547727 1105 8 0.01538739517875598 1105 9 0.07276239204258417 1105 10-11 0.010091475707007191 1105 12-13 0.054528589671086536 1105 14-15 0.04559886449512618 1105 16-17 -0.08517060449587177 1105 18-19 -0.12479212936090534 1105 20-21 -0.0929300869957288 1105 22-23 0.0095374306316387 1105 24-25 -0.11720998830438845 1105 26-27 0.06659495216519673 1105 28-29 0.033018475889690535 1105 30-31 0.028186323087354026 1105 32-33 0.0573955549858951 1105 34-35 0.18522852195670225 1105 36-37 0.23571136801059112 1105 38-39 0.06888947225753839 1105 40-41 0.1468122262966034 1105 42-43 0.1956722456833262 1105 44-45 0.32392161575731393 1105 46-47 0.35605696516316954 1105 48-49 0.3285210454399703 1105 50-51 0.306687811387242 1105 52-53 0.4174173715369136 1105 54-55 0.43355744546161645 1105 56-57 0.4261052899638571 1105 58-59 0.5732713082859675 1105 60-61 0.4031889147758676 1105 62-63 0.37919923365496544 1105 64-65 0.4483049951559934 1105 66-67 0.4656284356462095 1105 68-69 0.45997621952937706 1105 70-71 0.39575107143322796 1105 72-73 0.3623941344361583 1105 74-75 0.2795456090411328 1105 76-77 0.35053254757306007 1105 78-79 0.3082201554657722 1105 80-81 0.5355350485677839 1105 82-83 0.5601601859147038 1105 84-85 0.521258048424297 1105 86-87 0.6152215018783842 1105 88-89 0.7461586334707775 1105 90-91 0.986427926658024 1105 92-93 0.6912363743762988 1105 94-95 0.7605310096725582 1105 96-97 0.9454676377643487 1105 98-99 1.0098646371569373 1105 100-101 1.447483450468578 1106 1 -0.1020323564573502 1106 2 -0.11784427548691667 1106 3 -0.005443538884350119 1106 4 0.0339963285037399 1106 5 -0.0833201912409649 1106 6 -0.05089875267491806 1106 7 0.001298847028955663 1106 8 -0.11371987828958652 1106 9 -0.05327362394502444 1106 10-11 -0.08266939373682902 1106 12-13 -0.09501325416817252 1106 14-15 0.04614743152080081 1106 16-17 -0.031372949355692015 1106 18-19 -0.08934684288966821 1106 20-21 0.04250514939259631 1106 22-23 -0.08781397370791666 1106 24-25 0.09433508432341142 1106 26-27 0.07367407902037115 1106 28-29 -0.002334286008689901 1106 30-31 0.05153142937495403 1106 32-33 0.10292103473776848 1106 34-35 0.055731608369072205 1106 36-37 0.13009357106853514 1106 38-39 0.07406008039124146 1106 40-41 0.30412433959772756 1106 42-43 0.23170777511929685 1106 44-45 0.33280895991124027 1106 46-47 0.4277611162462236 1106 48-49 0.33583666829386516 1106 50-51 0.3859490664830716 1106 52-53 0.5041788998195216 1106 54-55 0.5197396981318718 1106 56-57 0.292484851285721 1106 58-59 0.40163543395667034 1106 60-61 0.3490486794284564 1106 62-63 0.31538798697408055 1106 64-65 0.3659750748627246 1106 66-67 0.42286852335312375 1106 68-69 0.4034193779354318 1106 70-71 0.4105087408030492 1106 72-73 0.366088337532382 1106 74-75 0.5296381336139859 1106 76-77 0.4143856695994117 1106 78-79 0.44488891988783763 1106 80-81 0.22648662391827656 1106 82-83 0.5196421998556033 1106 84-85 0.29519140361257357 1106 86-87 0.49154905003303995 1106 88-89 0.7006654575568376 1106 90-91 0.622345128332082 1106 92-93 0.6495359083236458 1106 94-95 0.8092194738523872 1106 96-97 0.7513650423703133 1106 98-99 0.7973213360556528 1106 100-101 1.266847966178215 1107 1 -0.08731818380736911 1107 2 -0.08405228059773862 1107 3 -0.08965944584587149 1107 4 -0.07919092258677551 1107 5 0.06283202493798257 1107 6 0.038418093827239375 1107 7 0.010250298165807692 1107 8 0.025212035651875908 1107 9 0.03597838848097723 1107 10-11 -0.04141574780837942 1107 12-13 -0.08953773970582546 1107 14-15 -0.0928131438262767 1107 16-17 -0.014904501016985705 1107 18-19 0.02739086452995565 1107 20-21 -0.01662629537153748 1107 22-23 -0.10455618788650156 1107 24-25 0.0046663943591767065 1107 26-27 -0.03808899670820409 1107 28-29 0.07376668753030913 1107 30-31 0.050091836153441704 1107 32-33 0.20934083379957258 1107 34-35 0.22012591973234663 1107 36-37 0.08232330982674085 1107 38-39 0.08061583334810507 1107 40-41 -0.012907572208952445 1107 42-43 0.05031428525120418 1107 44-45 -0.05507453957918784 1107 46-47 -0.0871511165998271 1107 48-49 -0.36064794112431287 1107 50-51 -0.25535719783280797 1107 52-53 -0.3218916333300541 1107 54-55 -0.29393863155256383 1107 56-57 -0.3905202002376029 1107 58-59 -0.12894727416269092 1107 60-61 -0.13199703887380565 1107 62-63 -0.29702046454307407 1107 64-65 0.008580195015291281 1107 66-67 -0.1648001934935337 1107 68-69 0.011212750153401174 1107 70-71 -0.1729568303258624 1107 72-73 0.01463071727505394 1107 74-75 0.06137263490948186 1107 76-77 0.038694336729403034 1107 78-79 -0.046585399097399005 1107 80-81 -0.040969358092638686 1107 82-83 -0.031138892630206527 1107 84-85 -0.12073342681073385 1107 86-87 0.14370059257012358 1107 88-89 0.1371037501435346 1107 90-91 0.2610163223148483 1107 92-93 0.3754504443607196 1107 94-95 0.3480019136219248 1107 96-97 0.5768471272128188 1107 98-99 0.5132393668075608 1107 100-101 0.8920532411419053 1108 1 -0.1971229331726292 1108 2 -0.18202783918531296 1108 3 -0.09451683163126745 1108 4 -0.032795164161235846 1108 5 0.00722897127482014 1108 6 -0.06227684607160455 1108 7 -0.03524450305166482 1108 8 -0.17218564457985508 1108 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-0.7530508415153037 2316 70-71 -0.7052558511595954 2316 72-73 -0.895315116665163 2316 74-75 -1.1653883116637083 2316 76-77 -1.2048481950323549 2316 78-79 -1.1181486299338523 2316 80-81 -1.2363340514951524 2316 82-83 -1.3136598266718273 2316 84-85 -1.3446953814057814 2316 86-87 -1.756710530846597 2316 88-89 -1.914004991450561 2316 90-91 -2.183175597571047 2316 92-93 -2.439663562691038 2316 94-95 -2.328833370440723 2316 96-97 -2.304375030580527 2316 98-99 -2.8406702904705057 2316 100-101 -3.6997789787321764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 33.0 5 159.0 6 376.0 7 789.0 8 1157.0 9 1970.0 10 3201.0 11 4631.0 12 6236.0 13 8455.0 14 9989.0 15 12954.0 16 16976.0 17 22519.0 18 19619.0 19 13123.0 20 11778.0 21 12197.0 22 13254.0 23 14463.0 24 16759.0 25 19258.0 26 21993.0 27 26065.0 28 31232.0 29 37256.0 30 44421.0 31 52829.0 32 63634.0 33 78821.0 34 105411.0 35 158015.0 36 188890.0 37 286971.0 38 245865.0 39 21286.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.36448327318821 23.934525002457292 16.60311750446461 19.09787421988989 2 14.266855568753831 25.845608153651668 39.066422654006494 20.821113623588 3 18.503035744717653 30.241714973203543 32.64048744877896 18.61476183329984 4 10.879335824331234 21.136388493929783 40.72514491961063 27.259130762128354 5 9.755398080625922 42.105283238670324 35.72322452810512 12.416094152598633 6 24.14078377409074 42.508565454827554 18.48357747317175 14.867073297909952 7 22.695784549878038 37.7452950408115 23.411925249714486 16.14699515959598 8 23.354440682794564 35.04650908434207 21.668693040043344 19.930357192820026 9 24.52212774291681 19.415794011939493 23.87339151314133 32.18868673200237 10-11 21.88979241966786 29.5219814879346 28.83858918628815 19.749636906109387 12-13 24.806084970329316 28.71379553299131 28.517972829639653 17.962146667039722 14-15 21.442822245955163 29.804847603462182 29.652360450886324 19.099969699696327 16-17 17.10236645075628 32.79992699829451 30.52240732662413 19.575299224325086 18-19 16.726085459411184 31.8954441211641 33.97420631955663 17.404264099868083 20-21 18.41547352276109 29.927998036367892 33.44250525882045 18.21402318205057 22-23 17.929843392708616 29.99998092326319 33.4616137901915 18.60856189383669 24-25 18.372709837745997 30.697076680851282 33.651808856184296 17.27840462521843 26-27 18.07884420337086 29.82532056886865 35.15736728146789 16.938467946292594 28-29 16.80209030164136 31.074573507754057 34.81625287104889 17.30708331955569 30-31 17.547100463183167 30.62000666414006 34.02940106677112 17.80349180590565 32-33 17.42818880373599 31.215328030848355 33.97996052387263 17.376522641543026 34-35 18.267464813453433 30.169395272169496 34.012061233367 17.551078681010075 36-37 18.208437597287848 29.721467769360345 34.486048266539214 17.584046366812597 38-39 17.500374422030706 30.833360096691507 34.36008174642519 17.306183734852603 40-41 17.12970676706074 30.898649894751568 34.59134355504398 17.380299783143716 42-43 16.97763875305079 31.11958217968105 33.177841990865744 18.724937076402412 44-45 17.68297995056478 30.403710375991295 33.065702296187816 18.84760737725611 46-47 18.431727406902453 31.02057077232125 32.51497070720495 18.032731113571344 48-49 18.046121841249068 30.771358559782218 32.66733244499301 18.515187153975702 50-51 17.16939936925648 30.94846490356505 33.327186355489694 18.554949371688778 52-53 17.535686215658693 31.961164850985252 32.169228460456466 18.333920472899585 54-55 17.32097754286543 30.629227086931415 32.52198911862932 19.527806251573832 56-57 18.349658780767598 30.915727879251886 31.610725195790906 19.123888144189607 58-59 18.608020664026434 30.367426179011918 32.003868781906 19.02068437505564 60-61 18.08011527524126 31.127971542378656 32.38472475874103 18.407188423639056 62-63 17.82762387796993 30.956202355849815 32.58185827765046 18.634315488529793 64-65 17.459188501051763 31.457380026402205 32.19663537233975 18.886796100206283 66-67 17.67775026811062 30.85063798087027 31.243782106902774 20.227829644116333 68-69 17.23275743386599 31.10356302224969 31.77863051469127 19.885049029193045 70-71 18.334721192853333 30.83588569124784 31.356913273246807 19.472479842652024 72-73 18.07075688860966 29.983288778554694 31.992228137423723 19.953726195411917 74-75 17.30511205675202 31.924060597890367 30.90879666487769 19.86203068047992 76-77 17.670973361376337 30.256813682387868 31.573930956042943 20.49828200019285 78-79 17.545683751046816 30.393621497801725 31.86057776868833 20.200116982463125 80-81 17.971786133179194 30.72182261744994 31.063200931199408 20.243190318171457 82-83 17.673674611915587 30.428698788500036 31.63298138873557 20.264645210848816 84-85 17.300915174653884 30.35840101335626 31.213229589799283 21.12745422219057 86-87 17.322956990308636 30.81050480556456 31.087159880461062 20.779378323665743 88-89 17.6551508130714 30.0770714630182 30.858789923170942 21.408987800739457 90-91 17.59616727249078 29.431702762137153 31.52923991336322 21.44289005200885 92-93 18.076607087897973 30.651292512507982 31.022176070650058 20.249924328943987 94-95 17.527516655612736 29.77951065533167 31.12086216840594 21.572110520649655 96-97 17.043670797735334 29.710182942784712 31.254508867664953 21.991637391814997 98-99 17.499558055035184 30.13279333381661 30.125639548414913 22.242009062733292 100-101 17.385747179647296 29.29427751850931 31.095036395442673 22.224938906400727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 20453.0 1 12030.0 2 4101.5 3 4451.5 4 3441.0 5 3332.0 6 6171.5 7 11890.5 8 15929.0 9 15128.5 10 10830.0 11 6909.0 12 5961.5 13 5603.5 14 5136.5 15 4590.5 16 4269.0 17 4315.0 18 4353.5 19 4514.5 20 4906.5 21 5465.5 22 6141.5 23 6944.0 24 7866.5 25 9699.0 26 13376.5 27 17217.0 28 18414.0 29 20663.5 30 26196.5 31 30979.0 32 38068.5 33 47146.0 34 55486.0 35 62293.5 36 69291.5 37 74879.0 38 72097.0 39 66650.0 40 61860.5 41 56327.0 42 50504.5 43 48123.0 44 48730.5 45 47929.5 46 46199.0 47 54299.0 48 83889.0 49 80403.5 50 46741.5 51 29859.5 52 19964.5 53 15868.0 54 13687.5 55 12172.0 56 10848.0 57 9383.5 58 8333.0 59 7312.5 60 5826.5 61 4205.5 62 3100.5 63 2300.5 64 1636.0 65 1386.0 66 1159.5 67 847.0 68 643.5 69 461.5 70 356.5 71 306.5 72 253.5 73 212.0 74 170.0 75 110.0 76 73.0 77 54.5 78 33.5 79 22.5 80 14.5 81 8.5 82 7.0 83 4.0 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37072458533533087 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.8615105214562414E-4 16-17 0.001907673680970828 18-19 9.53836840485414E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.358912269902759E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007630694723883312 36-37 0.0168193229538928 38-39 0.01096912366558226 40-41 0.008012229460077477 42-43 0.0034338126257474903 44-45 0.0020984410490679107 46-47 0.0015897280674756899 48-49 3.1794561349513796E-5 50-51 4.76918420242707E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.087129815922207E-4 60-61 7.630694723883312E-4 62-63 0.0 64-65 0.0 66-67 0.001112809647232983 68-69 7.948640337378449E-4 70-71 0.0019394682423203417 72-73 0.0 74-75 0.0 76-77 0.09140936387985217 78-79 0.0359596488863001 80-81 3.1794561349513796E-5 82-83 0.0 84-85 0.0 86-87 0.14174015449613253 88-89 0.31540204858717685 90-91 0.2676466174402071 92-93 0.0 94-95 1.907673680970828E-4 96-97 3.1794561349513796E-5 98-99 1.2717824539805518E-4 100-101 5.723021042912483E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1572596.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.670909257590566 #Duplication Level Percentage of deduplicated Percentage of total 1 71.01741861385219 17.5206429033067 2 11.973563104059641 5.907973776605797 3 5.03670555330188 3.727803169881395 4 2.714210766012619 2.678481900570908 5 1.7183857154584359 2.1197069027807456 6 1.2268710487108843 1.816081458810672 7 0.8603925813243596 1.485866710983017 8 0.664308621640216 1.3111278178816665 9 0.5284159601113323 1.1732851981952346 >10 3.405527475645688 16.232183585150583 >50 0.43289293318310784 7.515777950670382 >100 0.3739812511050278 18.132971413990138 >500 0.03215107037683616 5.470515136981834 >1k 0.012346011024705087 6.633594500695324 >5k 0.001543251378088136 2.485051778151984 >10k+ 0.0012860428150734465 5.78893579534368 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21755 1.3833813643173454 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20804 1.32290810863057 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17555 1.1163070489814293 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16486 1.0483302768161689 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14628 0.9301816868413757 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8649 0.5499823222238897 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8296 0.5275353619111329 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6327 0.4023283793167476 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5690 0.361822108157467 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5128 0.3260850212006135 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5072 0.32252403032946797 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4916 0.3126041271884197 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4408 0.28030085285731365 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4340 0.27597679251377977 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4206 0.26745585007211004 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4109 0.26128770517030436 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3711 0.23597923433609141 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3654 0.2323546543422468 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3579 0.22758547013981978 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3434 0.21836504734846074 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3365 0.21397739788222786 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3153 0.20049650387003404 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2947 0.1873971445940343 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2860 0.18186489091921892 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2637 0.16768451655733577 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2483 0.15789179166168552 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2351 0.14949802746541388 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2348 0.1493072600973168 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2328 0.14803547764333624 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2219 0.14110426326914224 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2193 0.13945094607896752 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2093 0.13309203380906476 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1994 0.12679671066186102 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1900 0.12081933312815243 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1893 0.12037420926925924 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1728 0.10988200402391969 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 1707 0.1085466324472401 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1650 0.10492205245339553 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.1794561349513796E-5 0.0 16-17 0.0 0.0 0.0 5.087129815922208E-4 0.0 18-19 0.0 0.0 0.0 7.948640337378449E-4 0.0 20-21 0.0 0.0 0.0 0.0015897280674756899 0.0 22-23 0.0 0.0 0.0 0.0034656071870970036 0.0 24-25 0.0 0.0 0.0 0.00753531103983477 0.0 26-27 0.0 0.0 0.0 0.013608072257591905 0.0 28-29 0.0 0.0 0.0 0.0258171838158052 0.0 30-31 0.0 0.0 0.0 0.05856558200580442 0.0 32-33 0.0 0.0 0.0 0.11515990120793898 0.0 34-35 0.0 0.0 0.0 0.2009734222902767 0.0 36-37 0.0 0.0 0.0 0.32436811488773976 0.0 38-39 0.0 0.0 0.0 0.4902085468868037 0.0 40-41 0.0 0.0 0.0 0.673027274646508 0.0 42-43 0.0 0.0 0.0 0.8643033557251831 0.0 44-45 0.0 0.0 0.0 1.0826048139509448 0.0 46-47 0.0 0.0 0.0 1.3489796489371715 0.0 48-49 0.0 0.0 0.0 1.6241933719785628 3.1794561349513796E-5 50-51 0.0 0.0 0.0 1.9060839529033522 6.358912269902759E-5 52-53 0.0 0.0 0.0 2.176369518935569 6.358912269902759E-5 54-55 0.0 0.0 0.0 2.4731399545719306 6.358912269902759E-5 56-57 0.0 0.0 0.0 2.8363610234287764 6.358912269902759E-5 58-59 0.0 0.0 0.0 3.2182772943591362 6.358912269902759E-5 60-61 0.0 0.0 0.0 3.649856670117436 6.358912269902759E-5 62-63 0.0 0.0 0.0 4.070148976596659 6.358912269902759E-5 64-65 0.0 0.0 0.0 4.498517101658658 6.358912269902759E-5 66-67 0.0 0.0 0.0 4.978742156281715 6.358912269902759E-5 68-69 0.0 0.0 0.0 5.516229215895246 6.358912269902759E-5 70-71 0.0 0.0 0.0 6.1117731445329895 6.358912269902759E-5 72-73 0.0 0.0 0.0 6.700036118621693 6.358912269902759E-5 74-75 0.0 0.0 0.0 7.325212578437183 6.358912269902759E-5 76-77 0.0 0.0 0.0 7.946351128961284 9.538368404854138E-5 78-79 0.0 0.0 0.0 8.67059944194186 1.2717824539805518E-4 80-81 0.0 0.0 0.0 9.45074259377488 1.2717824539805518E-4 82-83 0.0 0.0 0.0 10.191651256902599 1.2717824539805518E-4 84-85 0.0 0.0 0.0 10.969886735054649 1.2717824539805518E-4 86-87 0.0 0.0 0.0 11.781124967887493 1.2717824539805518E-4 88-89 0.0 0.0 0.0 12.602664638597581 1.2717824539805518E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 455 0.0 35.46136 9 TTGGCGC 85 1.6430553E-5 33.4981 4 GTAAGCG 85 1.09139364E-10 30.70708 94-95 CGTCAGA 85 6.443338E-4 27.915085 2 GTATGGT 240 0.0 27.77873 1 CGGTCAA 35 0.0081839375 27.284075 88-89 TTCGGAC 35 0.008432084 27.117512 64-65 CCTTTAG 630 0.0 26.455936 1 TTAGGCA 700 0.0 25.761635 4 CTTTAGG 670 0.0 24.790264 2 GTACATG 10185 0.0 24.546745 1 TGACCGT 215 1.6807462E-9 24.279633 5 GGCATTC 685 0.0 24.247412 7 CATGGGG 2670 0.0 24.172163 4 CGTGCAA 220 2.1937012E-9 23.727821 9 ACCGTGC 220 2.1937012E-9 23.727821 7 ATGGGCG 120 1.7220798E-4 23.72782 5 ACATGGG 9980 0.0 23.347416 3 TAGGCAT 725 0.0 22.909622 5 CATATAG 480 0.0 22.73916 3 >>END_MODULE