##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938696_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1548929 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.821598020309516 31.0 31.0 34.0 30.0 34.0 2 32.0268889019445 33.0 31.0 34.0 30.0 34.0 3 32.0723441810438 33.0 31.0 34.0 30.0 34.0 4 35.75597590334999 37.0 35.0 37.0 33.0 37.0 5 35.68462660328524 37.0 35.0 37.0 33.0 37.0 6 35.73949483804616 37.0 35.0 37.0 33.0 37.0 7 35.65594614084958 37.0 35.0 37.0 33.0 37.0 8 35.65930136242526 37.0 35.0 37.0 33.0 37.0 9 37.220493644318104 39.0 37.0 39.0 33.0 39.0 10-11 37.1482721286773 39.0 37.0 39.0 33.0 39.0 12-13 37.0844670737006 39.0 37.0 39.0 33.0 39.0 14-15 38.126339554621296 40.0 37.0 41.0 33.0 41.0 16-17 38.067938556254035 40.0 37.0 41.0 33.0 41.0 18-19 38.263872972873514 40.0 37.5 41.0 33.0 41.0 20-21 38.28544497520545 40.0 37.5 41.0 33.0 41.0 22-23 38.166261978438 40.0 37.0 41.0 33.0 41.0 24-25 38.05681635504274 40.0 37.0 41.0 33.0 41.0 26-27 37.80985571320571 40.0 37.0 41.0 33.0 41.0 28-29 37.60948597385678 40.0 36.0 41.0 32.0 41.0 30-31 37.30155223383383 39.0 36.0 41.0 32.0 41.0 32-33 36.9874193717078 39.0 35.0 41.0 31.0 41.0 34-35 36.8300073792924 39.0 35.0 41.0 31.0 41.0 36-37 36.628254748926516 39.0 35.0 41.0 30.5 41.0 38-39 36.44198798008172 39.0 35.0 41.0 30.0 41.0 40-41 36.26930801863739 39.0 35.0 41.0 30.0 41.0 42-43 35.99902803808309 39.0 35.0 41.0 30.0 41.0 44-45 35.65241725088755 38.5 35.0 40.0 28.5 41.0 46-47 35.21574713882947 38.0 35.0 40.0 26.0 41.0 48-49 34.81351824389627 38.0 34.5 40.0 24.0 41.0 50-51 34.58036456157771 38.0 34.0 40.0 24.0 41.0 52-53 34.3931545603446 38.0 34.0 40.0 23.0 41.0 54-55 34.14563998737192 38.0 34.0 40.0 23.0 41.0 56-57 33.8616686110209 38.0 33.0 40.0 21.5 41.0 58-59 33.575101570181715 38.0 33.0 40.0 20.0 41.0 60-61 33.173320081165755 37.0 33.0 40.0 18.5 41.0 62-63 32.82341475948866 36.5 32.0 39.5 16.5 41.0 64-65 32.416877726480685 36.0 31.5 39.0 13.0 40.5 66-67 31.970860187910485 36.0 31.0 39.0 8.0 40.0 68-69 31.61995256076941 35.0 31.0 38.5 7.5 40.0 70-71 31.16381641766666 35.0 31.0 38.0 4.0 40.0 72-73 30.63223943770179 35.0 30.0 37.0 2.0 39.5 74-75 30.109851387636233 34.5 29.0 37.0 2.0 39.0 76-77 28.177487154027073 32.0 27.0 35.0 2.0 37.0 78-79 29.111874075570928 34.0 29.0 36.0 2.0 38.0 80-81 29.103976360440022 34.0 29.0 35.5 2.0 37.0 82-83 28.72572274132643 34.0 29.0 35.0 2.0 37.0 84-85 28.251670024901077 34.0 28.5 35.0 2.0 36.0 86-87 27.651378791410064 33.5 26.5 35.0 2.0 36.0 88-89 26.95193549865746 33.0 25.5 35.0 2.0 35.5 90-91 26.34377592517152 32.5 24.5 35.0 2.0 35.0 92-93 26.0011727458134 32.0 24.0 35.0 2.0 35.0 94-95 25.574931452635983 32.0 22.0 34.5 2.0 35.0 96-97 24.837625223622258 32.0 18.0 34.0 2.0 35.0 98-99 23.919859464184608 31.5 2.0 34.0 2.0 35.0 100-101 20.264605091647198 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.01835962004666669 1101 2 0.11327513690503821 1101 3 0.016958343201771697 1101 4 0.07062813774189891 1101 5 0.04136851262430241 1101 6 0.0812072093350551 1101 7 0.11029775862294144 1101 8 0.1218319057442443 1101 9 0.12529885268025964 1101 10-11 0.11074421323278472 1101 12-13 0.12470071524536763 1101 14-15 0.280604276873774 1101 16-17 0.32920590187525534 1101 18-19 0.35120620202584263 1101 20-21 0.3177015975113804 1101 22-23 0.1964706643804064 1101 24-25 0.1405962693342957 1101 26-27 -0.020750929333338775 1101 28-29 0.17276961139442193 1101 30-31 0.33446454474439236 1101 32-33 0.4007334246132572 1101 34-35 0.388952862096545 1101 36-37 0.36208313220824806 1101 38-39 0.4804281743693508 1101 40-41 0.43762983072814876 1101 42-43 0.6032982335444572 1101 44-45 0.4587539206691176 1101 46-47 0.586264680421877 1101 48-49 0.48208587515983226 1101 50-51 0.3504724376607413 1101 52-53 0.3239094214603355 1101 54-55 0.3802024949835001 1101 56-57 0.4351157438120197 1101 58-59 0.5087738255538312 1101 60-61 0.4909507305335836 1101 62-63 0.3747115664209417 1101 64-65 0.3709813046516288 1101 66-67 0.35711229860608285 1101 68-69 0.31875568329870774 1101 70-71 0.41897017482503074 1101 72-73 0.3501148408177208 1101 74-75 0.35091341638039 1101 76-77 0.43712560989600036 1101 78-79 0.2820927773567874 1101 80-81 0.37945396951912613 1101 82-83 0.6035979707085097 1101 84-85 0.6183905035846777 1101 86-87 0.7036867183917579 1101 88-89 0.671304786648939 1101 90-91 0.6395587079075966 1101 92-93 0.46300719785443434 1101 94-95 0.5529167004800293 1101 96-97 0.6019061391434768 1101 98-99 0.22772579527859094 1101 100-101 0.7987445798792407 1102 1 0.21144807226102813 1102 2 0.19098347023837192 1102 3 0.20452244576586764 1102 4 0.22160570184446016 1102 5 -0.0019167437859550773 1102 6 0.0792040042068507 1102 7 0.18093878426396515 1102 8 0.23579023907758057 1102 9 0.3570456475520558 1102 10-11 0.33862081579688663 1102 12-13 0.33094270242484924 1102 14-15 0.36587671277121103 1102 16-17 0.5332603890547389 1102 18-19 0.40642254817968393 1102 20-21 0.3661310846908705 1102 22-23 0.28212771566246175 1102 24-25 0.2346026795907079 1102 26-27 0.30549907066666293 1102 28-29 0.447080508830318 1102 30-31 0.3676135832059302 1102 32-33 0.5411420784594085 1102 34-35 0.5380073492760289 1102 36-37 0.5872834527210742 1102 38-39 0.5484970846257653 1102 40-41 0.6844727794460894 1102 42-43 0.7603495155957418 1102 44-45 0.6348276386178426 1102 46-47 0.8231797445244382 1102 48-49 0.9003230546470178 1102 50-51 0.9863458350966354 1102 52-53 0.8455360240244403 1102 54-55 0.9281191616501658 1102 56-57 0.9708689489402289 1102 58-59 0.9636616460666474 1102 60-61 0.9482904741233256 1102 62-63 0.8712820792414533 1102 64-65 1.060260150805476 1102 66-67 1.1468318498881303 1102 68-69 1.0866723499653723 1102 70-71 1.0729445337993901 1102 72-73 1.0035523408177234 1102 74-75 0.9551842497137208 1102 76-77 0.8440647124601064 1102 78-79 0.7963876491516579 1102 80-81 1.0811206361857906 1102 82-83 1.2364905989136368 1102 84-85 1.235281529225702 1102 86-87 1.1886867183917609 1102 88-89 1.0230355558797086 1102 90-91 1.085263836112727 1102 92-93 1.2071498260595597 1102 94-95 1.2464102902236185 1102 96-97 1.3766256904255272 1102 98-99 1.6983427824580772 1102 100-101 1.9791291952638552 1103 1 0.2290462356555807 1103 2 0.23316641830677298 1103 3 0.19437995051571022 1103 4 0.09793526169247002 1103 5 0.04080056147054023 1103 6 0.13454955518848521 1103 7 0.2251091452519347 1103 8 0.12180551773495552 1103 9 0.2920199192430033 1103 10-11 0.2933431479691464 1103 12-13 0.21508634555341644 1103 14-15 0.25710494266354544 1103 16-17 0.14486068987805112 1103 18-19 0.1830946903582813 1103 20-21 0.20631395359524163 1103 22-23 0.21620585055796937 1103 24-25 0.24932481385290117 1103 26-27 0.3456597736432272 1103 28-29 0.23102723143956183 1103 30-31 0.15661037706280467 1103 32-33 0.17384018643914345 1103 34-35 0.11044995535582558 1103 36-37 0.13071976526065754 1103 38-39 0.12903163972393372 1103 40-41 0.165432643283971 1103 42-43 0.14382798298016297 1103 44-45 0.2412054885228443 1103 46-47 0.11593880880563745 1103 48-49 0.045676917851572796 1103 50-51 0.1300172647989868 1103 52-53 0.06427434733032555 1103 54-55 0.032595887425976855 1103 56-57 -0.09691564573994782 1103 58-59 -0.056281751653429524 1103 60-61 0.18431089844250437 1103 62-63 2.5373851947563253E-4 1103 64-65 0.09056770305373618 1103 66-67 0.1691052428583646 1103 68-69 0.0691145602250316 1103 70-71 0.039336237409269614 1103 72-73 0.26526683575122334 1103 74-75 0.2481004545902259 1103 76-77 0.20372549460070033 1103 78-79 0.11021285497813338 1103 80-81 0.23289232712943075 1103 82-83 0.2113708633214877 1103 84-85 0.23059739369688614 1103 86-87 0.27248817500987244 1103 88-89 0.13126544821662733 1103 90-91 0.26562640736034737 1103 92-93 0.22410497963777232 1103 94-95 0.5348230514649117 1103 96-97 0.7838009200012053 1103 98-99 0.869362217226918 1103 100-101 1.3716576943138854 1104 1 0.1026081218888173 1104 2 0.14902782507708423 1104 3 0.08205346313560113 1104 4 0.054829957427585896 1104 5 -0.036815937334338855 1104 6 0.020309773437617196 1104 7 0.05740280411508536 1104 8 0.043564742799667044 1104 9 0.14796221082749383 1104 10-11 0.08272518924601968 1104 12-13 0.09882188976976636 1104 14-15 0.12850543485556898 1104 16-17 0.2202625602706263 1104 18-19 0.24717394396132164 1104 20-21 0.32575577451717663 1104 22-23 0.13903683476916484 1104 24-25 0.235002803660187 1104 26-27 0.17109150242844606 1104 28-29 0.25615464034396496 1104 30-31 0.07717546285854127 1104 32-33 0.09660811443956163 1104 34-35 0.21059264704278036 1104 36-37 0.3289669204630101 1104 38-39 0.42250840596571493 1104 40-41 0.35588872237413227 1104 42-43 0.3523780515758901 1104 44-45 0.43991603812155944 1104 46-47 0.48464143807282767 1104 48-49 0.5688311191631428 1104 50-51 0.3848810398112761 1104 52-53 0.39418444213858095 1104 54-55 0.4150143229404861 1104 56-57 0.3553183906275663 1104 58-59 0.702580690731665 1104 60-61 0.8600057346692296 1104 62-63 0.721061855916389 1104 64-65 0.6772468133364953 1104 66-67 0.5507950943050091 1104 68-69 0.7364769405360967 1104 70-71 0.4954693476951739 1104 72-73 0.548129729155189 1104 74-75 0.5047182137335717 1104 76-77 0.5910854940978219 1104 78-79 0.6049112223526514 1104 80-81 0.6447868892875306 1104 82-83 0.7024296497821219 1104 84-85 0.8457995193001473 1104 86-87 0.9247475124364222 1104 88-89 0.8772911390062568 1104 90-91 0.8071558956660745 1104 92-93 0.8784021523622876 1104 94-95 0.7976230693799486 1104 96-97 0.795030622187312 1104 98-99 1.2082985827062167 1104 100-101 1.8897805600281217 1105 1 0.1405941500868444 1105 2 0.2019975993304044 1105 3 0.20488377992275986 1105 4 0.05560778646459852 1105 5 0.04127388314424962 1105 6 0.1096336675793026 1105 7 0.1821401431274623 1105 8 0.18686690677368745 1105 9 0.25936150610672115 1105 10-11 0.13169929788459456 1105 12-13 0.22458094362312409 1105 14-15 0.14059837737899272 1105 16-17 0.22785079980211975 1105 18-19 0.24583132674670338 1105 20-21 0.18589559982367376 1105 22-23 0.19166099237649092 1105 24-25 0.2592452058414807 1105 26-27 0.2133827951879681 1105 28-29 0.28109957924414886 1105 30-31 0.22094183366917974 1105 32-33 0.43062188852735517 1105 34-35 0.4453031337108655 1105 36-37 0.3593022529063674 1105 38-39 0.49003603613904545 1105 40-41 0.5677406145294768 1105 42-43 0.6451626718650445 1105 44-45 0.6386235774689837 1105 46-47 0.4938740388419234 1105 48-49 0.5414371682974135 1105 50-51 0.5248385389261614 1105 52-53 0.4308512263097981 1105 54-55 0.46963784602323955 1105 56-57 0.6288325839000706 1105 58-59 0.7426204941209988 1105 60-61 0.7488625865383938 1105 62-63 0.750093845763999 1105 64-65 0.6236515189339471 1105 66-67 0.7494459249344629 1105 68-69 0.8178188910401083 1105 70-71 0.7079862262021095 1105 72-73 0.5734162614477398 1105 74-75 0.5760472438459026 1105 76-77 0.7317913183896927 1105 78-79 0.5807174823820453 1105 80-81 0.731366158112909 1105 82-83 0.9067490532064078 1105 84-85 0.7472974341052598 1105 86-87 0.8807327344510547 1105 88-89 0.9174734805245528 1105 90-91 0.7193543240682523 1105 92-93 0.8301483977087258 1105 94-95 1.1922124829351688 1105 96-97 1.3537672515743822 1105 98-99 1.401679525818178 1105 100-101 1.9171789173145015 1106 1 0.1124002355760787 1106 2 -0.00706307150446861 1106 3 0.05541519409006668 1106 4 0.10858810586640288 1106 5 -0.0922355280638314 1106 6 0.03779442163524749 1106 7 0.011136834233496984 1106 8 -0.06471593094070016 1106 9 -0.004966463965310197 1106 10-11 -0.012087784142174485 1106 12-13 -0.036329730542853156 1106 14-15 -5.0694297655695664E-5 1106 16-17 0.02606698564219556 1106 18-19 -0.05125360203359719 1106 20-21 -0.011762730056233295 1106 22-23 0.07624905935533377 1106 24-25 0.11353549494719317 1106 26-27 0.005871556955511892 1106 28-29 0.08654463131660606 1106 30-31 -0.07813272240040448 1106 32-33 -0.1267504032590523 1106 34-35 -0.10938715102866325 1106 36-37 -0.3176097145549761 1106 38-39 -0.395330040602758 1106 40-41 -0.17225977692197603 1106 42-43 -0.26083421383753347 1106 44-45 -0.3366124131798429 1106 46-47 -0.3084307445066372 1106 48-49 -0.2625585724096524 1106 50-51 -0.32299355399538143 1106 52-53 -0.460081929967032 1106 54-55 -0.29817197180503996 1106 56-57 -0.3807950516691605 1106 58-59 -0.20327733930812286 1106 60-61 -0.1511618415378564 1106 62-63 -0.06386188785177893 1106 64-65 -0.21500988271067456 1106 66-67 -0.3014852966688437 1106 68-69 -0.63410842395297 1106 70-71 -0.5980380987417426 1106 72-73 -0.39700581731755946 1106 74-75 -0.0322828054355746 1106 76-77 -0.047916929831181676 1106 78-79 -0.2661534843035547 1106 80-81 -0.21692780988367488 1106 82-83 -0.08171762016009865 1106 84-85 0.016098866215344287 1106 86-87 0.0940660602564769 1106 88-89 0.1764206868973801 1106 90-91 0.16013129497926926 1106 92-93 0.13564407499313447 1106 94-95 0.5099820452510393 1106 96-97 0.6251641197369224 1106 98-99 1.073444283067463 1106 100-101 1.3552093293284493 1107 1 0.16772916678839067 1107 2 0.11153384337269756 1107 3 0.06628861492009719 1107 4 0.010850104829536633 1107 5 -0.06504174378595451 1107 6 -0.03125930425085954 1107 7 -0.018538827676330527 1107 8 0.15417021420196164 1107 9 0.09479751322369623 1107 10-11 0.055970007339176675 1107 12-13 0.0725767198377838 1107 14-15 0.11177534461199912 1107 16-17 0.08516648358210688 1107 18-19 0.027708306886154332 1107 20-21 0.06333879613365667 1107 22-23 0.10907112362266247 1107 24-25 0.09090243083448968 1107 26-27 0.043614742308449195 1107 28-29 0.06398660335768369 1107 30-31 0.06073702847956497 1107 32-33 0.21222883219075328 1107 34-35 0.2126373244004114 1107 36-37 0.06120291789520138 1107 38-39 0.053433340844669885 1107 40-41 0.1071811127794291 1107 42-43 0.08771269967534323 1107 44-45 0.1951727878715701 1107 46-47 0.09153639875330555 1107 48-49 0.17121235812960123 1107 50-51 0.16283370823096277 1107 52-53 0.3165854643229409 1107 54-55 0.422366674087975 1107 56-57 0.3885648444626142 1107 58-59 0.4278562978079421 1107 60-61 0.5495280363123811 1107 62-63 0.5505295916792647 1107 64-65 0.3625331607557243 1107 66-67 0.21075753396773678 1107 68-69 0.29095717583602365 1107 70-71 0.4039395586750132 1107 72-73 0.35372180599184944 1107 74-75 0.4826920233455638 1107 76-77 0.2967792646989089 1107 78-79 0.4242234700471812 1107 80-81 0.29655595956887737 1107 82-83 0.09536031284398305 1107 84-85 0.20186735012122625 1107 86-87 0.3988981611778293 1107 88-89 0.2991798344866723 1107 90-91 0.6533359754162085 1107 92-93 0.9399685449650299 1107 94-95 0.8162921310196403 1107 96-97 0.8584742600772692 1107 98-99 1.0391590138013633 1107 100-101 1.489067006209126 1108 1 0.16980442585218825 1108 2 0.08555874648146755 1108 3 0.13430145129073168 1108 4 0.08417821565661399 1108 5 -0.042251688537334076 1108 6 0.10397955669304082 1108 7 0.15361834227501703 1108 8 0.06497186891183304 1108 9 0.04157948733106309 1108 10-11 0.06724477988528577 1108 12-13 0.1224465007673956 1108 14-15 0.1523442542076694 1108 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-1.7342285935257102 2316 74-75 -1.8655007250337512 2316 76-77 -1.7196537218833292 2316 78-79 -1.5739532599392483 2316 80-81 -1.5800788587637022 2316 82-83 -1.806517608157069 2316 84-85 -1.966132612188435 2316 86-87 -2.1282577260526843 2316 88-89 -2.4028356562415034 2316 90-91 -2.8954306083317185 2316 92-93 -2.6702144416172082 2316 94-95 -2.356960921897592 2316 96-97 -2.6941602944229572 2316 98-99 -2.509715929663134 2316 100-101 -2.7200248451401876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 26.0 5 146.0 6 408.0 7 726.0 8 1179.0 9 1872.0 10 3075.0 11 4492.0 12 6392.0 13 8323.0 14 10051.0 15 13185.0 16 17168.0 17 22510.0 18 20374.0 19 13492.0 20 12372.0 21 12889.0 22 14065.0 23 15315.0 24 17263.0 25 20144.0 26 22807.0 27 27397.0 28 32343.0 29 38879.0 30 45999.0 31 54183.0 32 65425.0 33 80964.0 34 108705.0 35 160529.0 36 191520.0 37 277911.0 38 211853.0 39 14930.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.2667453495565 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16.719488110817217 31.919313280337576 33.92440841381367 17.436790195031534 20-21 18.39219228253845 29.95708647717229 33.46499419921765 18.185727041071605 22-23 17.961152512477977 29.95941066375541 33.48423329926678 18.59520352449983 24-25 18.3564440301061 30.724110058479383 33.63720058208225 17.282245329332273 26-27 18.09943737654581 29.773397908005307 35.17992878192297 16.94723593352592 28-29 16.800382716057353 31.06730521541013 34.81679922062277 17.31551284790975 30-31 17.553354608248668 30.605114889062058 34.02615613756344 17.815374365125837 32-33 17.391952762198912 31.215052465284078 33.99933115074997 17.39366362176704 34-35 18.300223586828583 30.193777403590687 33.97293069195701 17.53306831762372 36-37 18.22356600810038 29.770338527499813 34.42491004508642 17.58118541931339 38-39 17.498397878258118 30.883160530177197 34.32550271109669 17.292938880467997 40-41 17.1372620927701 30.922258134913207 34.53740207048214 17.40307770183456 42-43 16.985116276667316 31.159486727195013 33.15831679371077 18.697080202426903 44-45 17.690164196912335 30.40005888156026 33.02703515848953 18.882741763037874 46-47 18.464889684282834 31.062845160298995 32.491423044990114 17.980842110428053 48-49 18.073571063795722 30.803855905202216 32.634868554849035 18.48770447615303 50-51 17.134258574795876 31.037865518690655 33.27625088044707 18.551625026066397 52-53 17.55661492553887 32.03971905749069 32.10024474975935 18.303421267211085 54-55 17.338044545618295 30.683523905873027 32.48160503160571 19.49682651690297 56-57 18.37028447987284 30.949384230052722 31.549869279421166 19.130462010653268 58-59 18.687357622707154 30.440212754487554 31.92747340649607 18.94495621630922 60-61 18.09893475293105 31.154837062295275 32.35682726237088 18.389400922402793 62-63 17.861720936253263 30.89688062993631 32.63496433156425 18.60643410224618 64-65 17.521909125068273 31.475733069575917 32.16806400421461 18.8342938011412 66-67 17.7117962855953 30.880887893158405 31.23715566861544 20.17016015263085 68-69 17.335506883806513 31.005955743499523 31.82826799231725 19.830269380376713 70-71 18.354926231214826 30.812804343650686 31.39301226171012 19.43925716342437 72-73 18.074295206558855 29.919092482612182 32.07929479014209 19.927317520686874 74-75 17.288744874657908 31.946206423697525 30.91594083881618 19.849107862828383 76-77 17.67510754718139 30.268245392646126 31.565400539972604 20.49124652019988 78-79 17.584039833827465 30.37804510656014 31.84382083052651 20.19409422908588 80-81 18.026896970483445 30.750872952310672 31.00053230964604 20.221697767559842 82-83 17.720765090648968 30.397901239868307 31.562103653881472 20.319230015601246 84-85 17.34027189109378 30.407171665066635 31.12944492613929 21.123111517700295 86-87 17.364360872859574 30.80266310379869 31.092722346710783 20.740253676630957 88-89 17.693574329186966 30.059866779896627 30.834565713215973 21.411993177700435 90-91 17.643435937493418 29.41008952606876 31.46928954213829 21.477184994299527 92-93 18.088577230772806 30.641941043417688 31.001611123108724 20.267870602700786 94-95 17.608848212596754 29.835472776890782 30.99753828950826 21.558140721004207 96-97 17.068401889954107 29.69889145805175 31.15293075559105 22.07977589640309 98-99 17.565524307440818 30.087111804349973 30.10748071732145 22.239883170887754 100-101 17.43941538167535 29.1268751920708 31.20717950969328 22.22652991656057 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 20388.0 1 11920.0 2 4032.5 3 4362.5 4 3288.0 5 3193.5 6 6017.5 7 11598.0 8 15442.5 9 14761.0 10 10651.5 11 6768.0 12 5815.0 13 5458.5 14 5068.0 15 4565.0 16 4139.0 17 4085.0 18 4150.0 19 4406.0 20 4862.0 21 5383.0 22 5989.0 23 6777.0 24 7644.5 25 9523.0 26 13271.0 27 16881.5 28 18214.0 29 20522.5 30 25894.0 31 30712.0 32 37557.0 33 46745.0 34 55267.0 35 61810.0 36 68623.0 37 74030.0 38 71022.0 39 65871.5 40 61311.0 41 55542.5 42 49701.0 43 47291.5 44 47905.0 45 46971.0 46 45322.5 47 53563.0 48 81771.5 49 78202.0 50 45841.5 51 29234.5 52 19603.0 53 15773.0 54 13667.0 55 12072.5 56 10639.5 57 9186.5 58 8269.5 59 7195.0 60 5695.5 61 4220.5 62 3134.5 63 2363.0 64 1698.5 65 1362.0 66 1170.5 67 901.5 68 664.5 69 485.5 70 383.0 71 319.5 72 256.0 73 198.0 74 139.0 75 104.0 76 74.5 77 51.0 78 41.5 79 32.5 80 19.5 81 12.5 82 7.5 83 5.5 84 5.0 85 3.0 86 1.5 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2249941733933576 2 0.0 3 0.0 4 0.0 5 0.02317730509274473 6 0.0028406724904756772 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002614709906006021 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.519251689393123E-4 26-27 0.001581738091287593 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.005810466457791158 36-37 0.015268614636306762 38-39 0.013008988791610204 40-41 0.007166241964609094 42-43 0.007940970825647915 44-45 0.0036799620899343995 46-47 7.101681226189193E-4 48-49 0.002065943629436856 50-51 0.0 52-53 0.0 54-55 0.0 56-57 9.361307070885754E-4 58-59 4.8420553814926314E-4 60-61 0.002550149167586119 62-63 6.778877534089684E-4 64-65 0.0020013828910169546 66-67 0.006068709411470765 68-69 2.58242953679607E-4 70-71 9.684110762985262E-5 72-73 0.0 74-75 5.16485907359214E-4 76-77 0.057297655347662804 78-79 0.1357712328970534 80-81 0.0011943736607681822 82-83 9.361307070885754E-4 84-85 0.0 86-87 0.09596953766118396 88-89 0.3336498961540522 90-91 0.3638320413653563 92-93 6.133270149890666E-4 94-95 3.873644305194105E-4 96-97 1.6140184604975438E-4 98-99 0.0 100-101 0.0013557755068179368 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1548929.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.48044276674955 #Duplication Level Percentage of deduplicated Percentage of total 1 70.77818577526406 17.326813260057193 2 11.944207226804341 5.847989628199601 3 5.0849122405555 3.7344270923658947 4 2.8495084055516027 2.7902890974191115 5 1.7619949242987047 2.1567207949798823 6 1.224619810189617 1.7987541104624774 7 0.8585152659984925 1.4711783683559803 8 0.6424108679763046 1.2581201988985464 9 0.518267584041444 1.1418677936089323 >10 3.47334761877481 16.442492647430264 >50 0.4374245549367322 7.518402102750035 >100 0.3786191730738464 18.132570156376467 >500 0.03243047231727773 5.469023163396545 >1k 0.013183118828161679 7.247451309188109 >5k 0.0010546495062529343 1.8331629139703918 >10k+ 0.0013183118828161678 5.830737362540591 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21417 1.3826973347390359 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20829 1.3447356205481336 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17366 1.1211617834000138 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16378 1.057375773841151 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14345 0.9261237926334907 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8713 0.562517713852604 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8150 0.5261700181221992 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6171 0.39840431678921373 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5367 0.3464974830996127 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4959 0.3201567018242928 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4896 0.31608937530383896 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4825 0.31150556287602593 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4448 0.287166164491723 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4326 0.27928975440449494 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4104 0.2649572704752768 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3978 0.2568226174343692 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3827 0.247073945932964 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3612 0.23319338717268512 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3544 0.22880325696013182 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3471 0.224090323055479 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3289 0.21234026866305689 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3038 0.19613552331966153 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2799 0.180705506837305 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2706 0.17470135816425414 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2496 0.16114360309607478 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2459 0.1587548557745384 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2447 0.15798012691349958 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2238 0.14448693258374012 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2170 0.1400968023711868 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2152 0.13893470907962857 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2076 0.13402809295971604 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2033 0.13125198120766027 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1858 0.11995385198417745 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1839 0.11872719795419932 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1838 0.11866263721577942 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1711 0.11046342343645191 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1585 0.10232877039554426 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.456073841990175E-5 0.0 10-11 0.0 0.0 0.0 6.456073841990175E-5 0.0 12-13 0.0 0.0 0.0 1.9368221525970525E-4 0.0 14-15 0.0 0.0 0.0 4.8420553814926314E-4 0.0 16-17 0.0 0.0 0.0 5.487662765691649E-4 0.0 18-19 0.0 0.0 0.0 6.778877534089683E-4 0.0 20-21 0.0 0.0 0.0 0.0012589343991880842 0.0 22-23 0.0 0.0 0.0 0.003098915444155284 0.0 24-25 0.0 0.0 0.0 0.0059718683038409125 0.0 26-27 0.0 0.0 0.0 0.011588652546372365 0.0 28-29 0.0 0.0 0.0 0.02288678176985517 0.0 30-31 0.0 0.0 0.0 0.05529627245664585 0.0 32-33 0.0 0.0 0.0 0.10859116202227476 0.0 34-35 0.0 0.0 0.0 0.18670965551035584 0.0 36-37 0.0 0.0 0.0 0.2989162188841451 0.0 38-39 0.0 0.0 0.0 0.45809071945841284 0.0 40-41 0.0 0.0 0.0 0.6278854615027545 0.0 42-43 0.0 0.0 0.0 0.8007791189912514 0.0 44-45 0.0 0.0 0.0 1.0083741733804454 0.0 46-47 0.0 0.0 0.0 1.2602256139564822 0.0 48-49 0.0 0.0 0.0 1.5183394461592494 0.0 50-51 0.0 0.0 0.0 1.7920446966904229 0.0 52-53 0.0 0.0 0.0 2.0616180599627225 0.0 54-55 0.0 0.0 0.0 2.3482031778086663 0.0 56-57 0.0 0.0 0.0 2.6967988849069258 0.0 58-59 0.0 0.0 0.0 3.0706055603581572 0.0 60-61 0.0 0.0 0.0 3.500450956757863 0.0 62-63 0.0 0.0 0.0 3.912380748246046 0.0 64-65 0.0 0.0 0.0 4.325504913394997 0.0 66-67 0.0 0.0 0.0 4.792537295124566 0.0 68-69 0.0 0.0 0.0 5.319417481369385 0.0 70-71 0.0 0.0 0.0 5.900109043087191 0.0 72-73 0.0 0.0 0.0 6.486159146093849 0.0 74-75 0.0 0.0 0.0 7.087251901152344 0.0 76-77 0.0 0.0 0.0 7.691120767962896 0.0 78-79 0.0 0.0 0.0 8.420818513953835 0.0 80-81 0.0 0.0 0.0 9.196612627176584 0.0 82-83 0.0 0.0 0.0 9.930635942641658 0.0 84-85 0.0 0.0 0.0 10.70433183186576 0.0 86-87 0.0 0.0 0.0 11.502270278366536 0.0 88-89 0.0 0.0 0.0 12.316155227257028 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGTCG 25 0.005044225 56.935307 9 TAGCGCG 20 5.419861E-4 47.44609 12-13 CGTCTAG 70 2.0385615E-4 33.971214 1 GCGCGTT 30 0.003974006 31.630726 14-15 AGCGCGT 30 0.003974006 31.630726 12-13 GTAAGCG 110 0.0 30.193941 94-95 GGGACCG 65 0.0058772136 29.197594 7 GTATGGT 275 0.0 27.671099 1 CGCGTTC 35 0.008440348 27.112053 14-15 CTTTGCG 220 7.8216544E-11 25.885536 2 CATGGGT 865 0.0 25.785786 4 CATGGGG 2390 0.0 25.614767 4 CATTCCG 390 0.0 24.331327 9 GTACATG 9855 0.0 24.322771 1 TTGACGG 50 0.0016636824 23.726875 30-31 TCGTATC 90 1.9741674E-7 23.723812 94-95 ACATGGG 9615 0.0 23.099104 3 TACATGG 10100 0.0 23.023603 2 GTACACG 105 0.002180586 22.647476 1 GAGTACT 12740 0.0 22.643032 12-13 >>END_MODULE