##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938695_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1827410 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.782743883419702 33.0 31.0 34.0 30.0 34.0 2 31.79395537947149 33.0 31.0 34.0 30.0 34.0 3 31.785448804592292 33.0 31.0 34.0 30.0 34.0 4 35.35526072419435 37.0 35.0 37.0 33.0 37.0 5 35.412974647178245 37.0 35.0 37.0 33.0 37.0 6 35.54350036390301 37.0 35.0 37.0 33.0 37.0 7 35.48961973503483 37.0 35.0 37.0 33.0 37.0 8 35.47604642636299 37.0 35.0 37.0 33.0 37.0 9 37.029536338314884 39.0 37.0 39.0 33.0 39.0 10-11 36.934613742947676 39.0 37.0 39.0 33.0 39.0 12-13 36.81396320475427 39.0 37.0 39.0 33.0 39.0 14-15 37.86075128186887 40.0 38.0 41.0 33.0 41.0 16-17 37.82463459212765 40.0 37.5 41.0 32.5 41.0 18-19 37.88281474874276 40.0 37.5 41.0 33.0 41.0 20-21 37.805893860709965 40.0 37.5 41.0 33.0 41.0 22-23 37.70787863697802 40.0 37.0 41.0 32.5 41.0 24-25 37.69905056883786 40.0 37.0 41.0 32.5 41.0 26-27 37.523565045611 40.0 37.0 41.0 32.0 41.0 28-29 37.37531779950859 40.0 36.5 41.0 32.0 41.0 30-31 37.12493392287445 39.0 36.0 41.0 32.0 41.0 32-33 36.835508178241334 39.0 35.0 41.0 31.0 41.0 34-35 36.49874686031049 39.0 35.0 41.0 30.5 41.0 36-37 36.195303188665925 39.0 35.0 41.0 30.0 41.0 38-39 35.968156571322254 38.0 35.0 40.5 30.0 41.0 40-41 35.75866636387018 38.0 35.0 40.0 30.0 41.0 42-43 35.51530827783584 38.0 35.0 40.0 28.5 41.0 44-45 35.362636463628846 38.0 35.0 40.5 27.0 41.0 46-47 35.02982417738767 38.0 35.0 41.0 24.5 41.0 48-49 34.738285606404695 38.0 35.0 41.0 24.0 41.0 50-51 33.566224602032385 37.0 33.0 39.5 21.5 40.5 52-53 33.65244581128482 37.5 33.5 39.5 22.5 40.5 54-55 34.19415511571022 38.0 34.0 40.0 23.0 41.0 56-57 34.156051734421936 38.0 34.0 40.0 22.0 41.0 58-59 33.97695098527424 38.0 34.0 40.0 22.0 41.0 60-61 33.66733026523878 37.5 34.0 40.0 20.5 41.0 62-63 33.25388965804061 37.0 33.0 40.0 18.5 41.0 64-65 32.812792969284395 36.0 33.0 39.0 16.0 41.0 66-67 32.41202439518226 36.0 32.0 39.0 12.5 41.0 68-69 32.04230851314155 35.0 32.0 39.0 8.0 40.0 70-71 31.594861032827882 35.0 31.5 37.5 7.0 40.0 72-73 31.128679661378673 35.0 31.0 37.0 2.0 39.5 74-75 30.685855390963166 35.0 31.0 37.0 2.0 39.0 76-77 30.213473440552477 35.0 30.5 36.0 2.0 39.0 78-79 29.80515948801856 34.5 30.0 36.0 2.0 37.5 80-81 29.428983096294758 34.0 29.5 35.0 2.0 37.0 82-83 29.001308135557977 34.0 29.0 35.0 2.0 37.0 84-85 28.612348077333493 34.0 29.0 35.0 2.0 36.0 86-87 28.225234895288963 34.0 29.0 35.0 2.0 36.0 88-89 27.814510427326105 34.0 27.5 35.0 2.0 35.5 90-91 27.376832511587438 34.0 26.5 35.0 2.0 35.0 92-93 26.97201996267942 34.0 25.5 35.0 2.0 35.0 94-95 26.51551293907771 33.5 25.0 35.0 2.0 35.0 96-97 25.915296238939263 33.0 23.5 35.0 2.0 35.0 98-99 25.253559135607226 33.0 19.0 35.0 2.0 35.0 100-101 23.14395100169092 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1824938958548472 1101 2 0.022404161751385487 1101 3 0.22880699147327377 1101 4 0.26015831909203513 1101 5 0.031195918505076747 1101 6 -0.024504749494610678 1101 7 0.1574340303222641 1101 8 0.08042358529718285 1101 9 0.20771005457834235 1101 10-11 0.24039223488326655 1101 12-13 0.2211806164382395 1101 14-15 0.3620811464165712 1101 16-17 0.30636568523569707 1101 18-19 0.34176722868408405 1101 20-21 0.44089022908963926 1101 22-23 0.45977569351479275 1101 24-25 0.3870474639606627 1101 26-27 0.40680721573804846 1101 28-29 0.34423366710862524 1101 30-31 0.43525807566494024 1101 32-33 0.38380692194088084 1101 34-35 0.43614411597407354 1101 36-37 0.556755888077646 1101 38-39 0.49067252685571816 1101 40-41 0.4447964604506751 1101 42-43 0.296002783960013 1101 44-45 0.5336768689568885 1101 46-47 0.6567516605015626 1101 48-49 0.7460447113300859 1101 50-51 0.6037516361776909 1101 52-53 0.6384821110445955 1101 54-55 0.5742488844568001 1101 56-57 0.5763023743337143 1101 58-59 0.5608610944541041 1101 60-61 0.3650275701221588 1101 62-63 0.3739101927091113 1101 64-65 0.36022416289198134 1101 66-67 0.18466523994085549 1101 68-69 0.13953179296606777 1101 70-71 0.03885665554022921 1101 72-73 0.1139320381960367 1101 74-75 0.15940404411270848 1101 76-77 0.17178671357277508 1101 78-79 0.1548797262318793 1101 80-81 0.10176872441239126 1101 82-83 -0.08221507137615447 1101 84-85 -0.08547173015216813 1101 86-87 -0.0997537378649831 1101 88-89 0.036381700250963434 1101 90-91 -0.16818080415498926 1101 92-93 -0.3559884252108567 1101 94-95 -0.6218355498073045 1101 96-97 -0.7358619851114945 1101 98-99 -0.5529439379483669 1101 100-101 -0.43433255997792486 1102 1 0.35170754253092795 1102 2 0.3526582890005052 1102 3 0.4125633945952849 1102 4 0.3801246447344866 1102 5 0.3303298026139174 1102 6 0.30203473464086983 1102 7 0.33175971082491174 1102 8 0.32200190433793807 1102 9 0.5066299067536164 1102 10-11 0.3062926672277726 1102 12-13 0.3761820670991156 1102 14-15 0.3122407191137029 1102 16-17 0.3467720605335529 1102 18-19 0.46466008429813854 1102 20-21 0.40454094799912355 1102 22-23 0.39132542740176035 1102 24-25 0.404337439180523 1102 26-27 0.47298779210226627 1102 28-29 0.3976660258832183 1102 30-31 0.3350878013711309 1102 32-33 0.49102122498155865 1102 34-35 0.45981366969370896 1102 36-37 0.41072760732483005 1102 38-39 0.27269241755674045 1102 40-41 0.4462804151900528 1102 42-43 0.2078853612849869 1102 44-45 0.4001066736170742 1102 46-47 0.5627388471231427 1102 48-49 0.43455785526893465 1102 50-51 0.4271809985052073 1102 52-53 0.4665474050531273 1102 54-55 0.354884226360916 1102 56-57 0.4221437338645515 1102 58-59 0.3819950832007777 1102 60-61 0.43844670647952455 1102 62-63 0.499721968270471 1102 64-65 0.5123780375834173 1102 66-67 0.4643540850721024 1102 68-69 0.4041936938550137 1102 70-71 0.13664594347977044 1102 72-73 0.2786248548087187 1102 74-75 0.20936860993703732 1102 76-77 0.2990929076569344 1102 78-79 0.23611293067042638 1102 80-81 0.33165424110926267 1102 82-83 0.1487777038570215 1102 84-85 0.16628452076935574 1102 86-87 0.028523685571041568 1102 88-89 0.0043620473631946766 1102 90-91 0.07893428319382068 1102 92-93 -0.0412411493617455 1102 94-95 -0.17131402532918827 1102 96-97 0.03913195065038266 1102 98-99 0.0766053107995468 1102 100-101 -0.07493061276295876 1103 1 0.10988534498542535 1103 2 0.17307860807800424 1103 3 0.14676246595048426 1103 4 0.30359990166405737 1103 5 0.07444156175071726 1103 6 0.15479485589195008 1103 7 0.159937484999638 1103 8 0.2237897199676624 1103 9 0.17827475557782435 1103 10-11 0.3428332721069083 1103 12-13 0.3878957048924576 1103 14-15 0.47454098931119404 1103 16-17 0.529440677875904 1103 18-19 0.45381604942855347 1103 20-21 0.44269020045482677 1103 22-23 0.5232711352711092 1103 24-25 0.4257950287951857 1103 26-27 0.35075634229587394 1103 28-29 0.2558693743965037 1103 30-31 0.24647455644663552 1103 32-33 0.1600649634163105 1103 34-35 0.4348877234133326 1103 36-37 0.42707928448800203 1103 38-39 0.3796058157890059 1103 40-41 0.47779727606019406 1103 42-43 0.5151075117603909 1103 44-45 0.5103861500139928 1103 46-47 0.41192016349615557 1103 48-49 0.41189303370015296 1103 50-51 0.4173925846012452 1103 52-53 0.438306441303709 1103 54-55 0.3974968120525517 1103 56-57 0.5117118923519328 1103 58-59 0.37209559264512393 1103 60-61 0.5514429261027303 1103 62-63 0.4672841295613068 1103 64-65 0.4041155372181393 1103 66-67 0.416315554199862 1103 68-69 0.35472577772527103 1103 70-71 0.3200085149529599 1103 72-73 0.19784007862581632 1103 74-75 0.2341207927424982 1103 76-77 0.22793346537170223 1103 78-79 0.27740422266942133 1103 80-81 0.20588891983693358 1103 82-83 0.18559843556778333 1103 84-85 0.32627433376780957 1103 86-87 0.1673435770584213 1103 88-89 0.26281243537734866 1103 90-91 0.14719190686989592 1103 92-93 0.029247433938042633 1103 94-95 0.1634135006591393 1103 96-97 0.20132208685518194 1103 98-99 0.22047973982313707 1103 100-101 0.27716383412716183 1104 1 0.12040588207332803 1104 2 0.24071497673071818 1104 3 0.18224121842220953 1104 4 0.16524618575680705 1104 5 0.08654883260312829 1104 6 0.2273977231741 1104 7 0.2149696507673795 1104 8 0.23080759165009113 1104 9 0.18612565464857767 1104 10-11 0.16690741875547133 1104 12-13 0.23109217237978186 1104 14-15 0.31431787466662087 1104 16-17 0.33857096530326913 1104 18-19 0.293917743355415 1104 20-21 0.2541063752319346 1104 22-23 0.3507987244045694 1104 24-25 0.2768867586861319 1104 26-27 0.29915034261754414 1104 28-29 0.2815016616225918 1104 30-31 0.23379228663668528 1104 32-33 0.28954115316094686 1104 34-35 0.2011235275387122 1104 36-37 0.3191367116466992 1104 38-39 0.34262419663081545 1104 40-41 0.2272938384505565 1104 42-43 0.3711202796216426 1104 44-45 0.47629896399431004 1104 46-47 0.4827704439884215 1104 48-49 0.3502576288134094 1104 50-51 0.4176423047046711 1104 52-53 0.5380687604369072 1104 54-55 0.38854357037835285 1104 56-57 0.3369239663267649 1104 58-59 0.38915196166779253 1104 60-61 0.4292111275317012 1104 62-63 0.5104593716236465 1104 64-65 0.35556198565900843 1104 66-67 0.15498119293309287 1104 68-69 0.33622783552551994 1104 70-71 0.33901342188244854 1104 72-73 0.3986923248996881 1104 74-75 0.2825257349745378 1104 76-77 0.21239093629804273 1104 78-79 0.25758588346330313 1104 80-81 0.2565874307025027 1104 82-83 0.2835780698195407 1104 84-85 0.3138656117171017 1104 86-87 0.3094060989461589 1104 88-89 0.5018663708439064 1104 90-91 0.2963234044913641 1104 92-93 0.23398327458871293 1104 94-95 0.3261828824976334 1104 96-97 0.24927257925682866 1104 98-99 0.2819996119313295 1104 100-101 0.44753047591065 1105 1 0.019005692433200494 1105 2 0.03323795727736112 1105 3 0.12736352829935882 1105 4 0.03597924433059774 1105 5 0.12146996318843861 1105 6 0.052932190837502446 1105 7 0.057767283068166364 1105 8 0.1007809194648317 1105 9 0.16312339701331524 1105 10-11 0.0295043983714649 1105 12-13 0.05521126810615584 1105 14-15 0.15769908612768546 1105 16-17 0.018066980797222243 1105 18-19 0.11565263335151599 1105 20-21 0.08263357233048652 1105 22-23 0.23811805738128555 1105 24-25 0.10216993315967215 1105 26-27 0.07386307231137579 1105 28-29 -0.0015674905061757727 1105 30-31 -0.004568234710454533 1105 32-33 0.13048411237846835 1105 34-35 0.04669585547755872 1105 36-37 0.19847979552534412 1105 38-39 0.30204388797749004 1105 40-41 0.1768171666810332 1105 42-43 0.25695864574782235 1105 44-45 0.1824602357103018 1105 46-47 0.3218710484162912 1105 48-49 0.43026663964519685 1105 50-51 0.4730663111495943 1105 52-53 0.3711313992679308 1105 54-55 0.23060841818737998 1105 56-57 0.16328560740679876 1105 58-59 0.31604073332758276 1105 60-61 0.4076924543843816 1105 62-63 0.19121231500291458 1105 64-65 0.33093679283922484 1105 66-67 0.1848084011489064 1105 68-69 0.09367801649665353 1105 70-71 0.09557314420347751 1105 72-73 0.13878141079915096 1105 74-75 0.3817945518609527 1105 76-77 0.13341050298359036 1105 78-79 0.07333377480240344 1105 80-81 0.15539634409658376 1105 82-83 0.18810836866442315 1105 84-85 -0.053798497979766324 1105 86-87 0.0628020215293823 1105 88-89 -0.10642113073822657 1105 90-91 0.0015780497968762575 1105 92-93 0.06511979423346759 1105 94-95 0.2067990620219824 1105 96-97 0.14919568230889624 1105 98-99 0.2985258346528532 1105 100-101 0.5128001864892617 1106 1 0.13654901841217182 1106 2 0.21284713776704578 1106 3 0.17365996033505127 1106 4 0.11127470148595364 1106 5 0.15660444292240783 1106 6 0.16257935256746237 1106 7 0.20463428886613855 1106 8 0.1251987399842278 1106 9 0.23042740086396662 1106 10-11 0.33883344125398906 1106 12-13 0.20143934856921675 1106 14-15 0.21086106408414906 1106 16-17 0.07410778772449333 1106 18-19 0.25256865193043865 1106 20-21 0.31840099129203026 1106 22-23 0.3033183720795023 1106 24-25 0.23029629988571543 1106 26-27 0.19441624971272375 1106 28-29 0.1998372289897219 1106 30-31 0.06791665342263542 1106 32-33 0.2484625000876477 1106 34-35 0.16161460934103644 1106 36-37 0.22313828199308006 1106 38-39 0.24901339059305627 1106 40-41 0.22174824703902374 1106 42-43 0.18238091064650774 1106 44-45 0.03470523975397555 1106 46-47 -0.03416902551824563 1106 48-49 0.01912979234468537 1106 50-51 -0.09669077505768087 1106 52-53 -0.04131475630733661 1106 54-55 0.07745981734834828 1106 56-57 0.061579963851393416 1106 58-59 -0.20701906544147164 1106 60-61 -0.05164584253142834 1106 62-63 -0.10987010147866982 1106 64-65 -0.14058017838592463 1106 66-67 -0.11107694412731917 1106 68-69 0.17921796337911644 1106 70-71 0.1127352528769805 1106 72-73 0.0766133954258521 1106 74-75 -0.019587383777402323 1106 76-77 -0.015176745141783243 1106 78-79 0.0964905424021687 1106 80-81 0.01520171009207516 1106 82-83 0.0940191304637743 1106 84-85 0.045562790474864556 1106 86-87 0.23426643106708056 1106 88-89 0.17170792362784582 1106 90-91 0.2623509051212132 1106 92-93 0.347994060324492 1106 94-95 0.3632876664282456 1106 96-97 0.5690468970000282 1106 98-99 0.5518101002307816 1106 100-101 0.6273816109873458 1107 1 0.14648734921000184 1107 2 0.03269694225692987 1107 3 0.12598829716521465 1107 4 0.1002747038892764 1107 5 0.07476747697025132 1107 6 0.18212675926455546 1107 7 0.01694788132547842 1107 8 0.0775018419499034 1107 9 0.04615340791531253 1107 10-11 0.07181613013694488 1107 12-13 0.06070901554110719 1107 14-15 0.13966494953228192 1107 16-17 0.15695670178416066 1107 18-19 0.2764098909863222 1107 20-21 0.2343860162024427 1107 22-23 0.3122761481429066 1107 24-25 0.32695168897127047 1107 26-27 0.38748249003034374 1107 28-29 0.27823985005097995 1107 30-31 0.36869445803749556 1107 32-33 0.38775309397215807 1107 34-35 0.3157282827922856 1107 36-37 0.2898346296670269 1107 38-39 0.43255286873605314 1107 40-41 0.37069207783565616 1107 42-43 0.3744837198877562 1107 44-45 0.42274760909145925 1107 46-47 0.3883210367517904 1107 48-49 0.46787898422819296 1107 50-51 0.6147900067905283 1107 52-53 0.5564490141178808 1107 54-55 0.44821990949164814 1107 56-57 0.42987575131135003 1107 58-59 0.4384630826943905 1107 60-61 0.41760682695673523 1107 62-63 0.5005635236177639 1107 64-65 0.4360925025901139 1107 66-67 0.4484647186339501 1107 68-69 0.41774601373347764 1107 70-71 0.4779849819025941 1107 72-73 0.2980867329890309 1107 74-75 0.36228941721084595 1107 76-77 0.4175586721106903 1107 78-79 0.3733497510848842 1107 80-81 0.20112618334431787 1107 82-83 0.42481651012351307 1107 84-85 0.3251180740546573 1107 86-87 0.3661249067370669 1107 88-89 0.1475958604012959 1107 90-91 0.3103365020218867 1107 92-93 0.2520554615564343 1107 94-95 0.23835721251311526 1107 96-97 0.2170749125062521 1107 98-99 0.35251159942206556 1107 100-101 0.4133791451805884 1108 1 0.147218479447357 1108 2 0.07403638696851544 1108 3 0.008386321995995871 1108 4 -0.01957536441523189 1108 5 0.13614212533807546 1108 6 0.23593865057142693 1108 7 0.23611872787488863 1108 8 0.27187043334780014 1108 9 0.19702974955841057 1108 10-11 0.39552389567530355 1108 12-13 0.24858095893291932 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2316 72-73 -1.7810764577093217 2316 74-75 -1.8830882584692823 2316 76-77 -1.8017482218455676 2316 78-79 -1.7348280912786258 2316 80-81 -1.8077351913695487 2316 82-83 -1.6746096391070466 2316 84-85 -1.593226639252368 2316 86-87 -1.542909104338733 2316 88-89 -1.6284094793384227 2316 90-91 -1.6781983069976043 2316 92-93 -1.9697875641221039 2316 94-95 -1.9392131896064697 2316 96-97 -2.0300898797878233 2316 98-99 -2.174867206508406 2316 100-101 -2.4584622087712518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6469.0 3 1723.0 4 1656.0 5 1746.0 6 2039.0 7 2367.0 8 2836.0 9 3507.0 10 4386.0 11 6230.0 12 8437.0 13 11845.0 14 12872.0 15 13153.0 16 17535.0 17 23060.0 18 19060.0 19 12707.0 20 12116.0 21 13046.0 22 13894.0 23 15554.0 24 17446.0 25 19886.0 26 23096.0 27 26861.0 28 31694.0 29 38088.0 30 45184.0 31 55253.0 32 68797.0 33 88584.0 34 123426.0 35 203218.0 36 239757.0 37 324280.0 38 270977.0 39 44612.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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20.832643291658922 18-19 19.180209170258458 29.359883164682227 32.86899415686538 18.59091350819394 20-21 21.061904555939233 27.61442408401329 31.408923099328597 19.914748260718884 22-23 20.194795487736165 27.524267299229066 30.72384916847504 21.557088044559727 24-25 20.509012165458117 27.833342506647913 31.88041721877698 19.777228109116987 26-27 21.39594974782451 26.356706323583133 32.86671694293972 19.38062698565264 28-29 19.605885675686554 26.888285354345026 34.00612618897872 19.499702780989704 30-31 19.521068873829872 29.02703066753692 32.56153438434953 18.890366074283687 32-33 19.25051286922615 28.81958226958936 31.78280843738999 20.1470964237945 34-35 20.000647614719732 27.460510594812167 32.0021009060569 20.536740884411202 36-37 21.115881503561486 27.292960951349237 32.58555851112811 19.00559903396117 38-39 21.764801099723446 26.579746301803404 32.74106673943925 18.9143858590339 40-41 19.71963564338155 27.601143086052094 33.70221789576442 18.97700337480194 42-43 19.195166668636308 29.10041233265733 31.959422486929608 19.74499851177675 44-45 19.447832104018328 29.29373138676902 30.81934635349025 20.439090155722393 46-47 19.655293541412767 28.599157941260383 29.963339275789902 21.782209241536947 48-49 20.45545387319975 28.43554563472973 30.23143808295924 20.877562409111277 50-51 21.2474897321593 28.941182356081185 30.46669404391024 19.344633867849275 52-53 19.726175236083556 29.277192457751262 31.059038138699723 19.937594167465456 54-55 18.699235707936314 29.034247738815843 31.539430334968056 20.72708621827979 56-57 20.100915139441902 30.00195938386858 29.36151841532288 20.53560706136664 58-59 19.86510709336229 28.71123092799594 30.211329289696636 21.212332688945136 60-61 19.974032979818563 28.12900670517132 31.032429385855526 20.864530929154586 62-63 21.66464854775137 27.767511601664552 30.496176933422962 20.07166291716112 64-65 20.813414756187818 28.28814606846602 31.30059373580681 19.597845439539352 66-67 19.65122158226266 28.668162868837065 31.175634944266335 20.50498060463394 68-69 19.309223749716104 29.96974891212117 30.646507706850954 20.074519631311773 70-71 19.681227074225003 29.671853987713533 29.372597098329972 21.274321839731495 72-73 19.735620494827685 28.859523163448525 29.006635880492016 22.398220461231773 74-75 19.906350675566642 29.4691601333072 29.636550122291734 20.987939068834425 76-77 20.825330405920205 27.990831861014577 30.493599673689452 20.690238059375766 78-79 19.8494325761912 27.961591210296206 31.44986201512599 20.73911419838661 80-81 19.517614066521112 29.990478140255757 30.145218666142714 20.346689127080417 82-83 19.489620444644522 29.48638563690396 29.462804795430703 21.56118912302081 84-85 19.69779585554884 28.353625020612323 29.4762546034189 22.472324520419942 86-87 20.59200976742179 28.32370911355755 30.11887416281107 20.965406956209595 88-89 21.290156582702508 27.045440925916658 30.461330983209184 21.203071508171647 90-91 20.004354384196883 27.71439088240331 31.344473849366345 20.936780884033457 92-93 19.575746346205232 29.20712845984757 30.314235696840875 20.902889497106326 94-95 19.449884754619713 29.23089653867978 29.605856400737217 21.713362305963287 96-97 19.5153446606893 28.11989589235575 29.05037102163118 23.31438842532377 98-99 20.362677094008824 27.848321538062294 29.151757634670624 22.637243733258266 100-101 21.36187645302573 27.570285352401175 29.70945257659359 21.358385617979504 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15701.0 1 9749.5 2 7061.0 3 8953.0 4 5817.0 5 4833.5 6 8117.0 7 15257.5 8 21309.0 9 20534.5 10 14273.5 11 8985.5 12 7746.0 13 7583.5 14 7276.5 15 6730.0 16 6352.5 17 6061.0 18 5648.0 19 5574.5 20 5814.0 21 6200.0 22 6546.5 23 7158.0 24 7815.5 25 8591.0 26 10407.5 27 12425.5 28 13645.5 29 15369.0 30 18090.0 31 20706.5 32 24503.0 33 29464.0 34 33727.5 35 36909.0 36 42008.5 37 46978.0 38 48173.5 39 48296.0 40 47625.0 41 46334.0 42 45097.0 43 45883.5 44 50111.0 45 53133.5 46 55897.0 47 67509.0 48 127617.5 49 128551.5 50 76807.0 51 64293.0 52 48983.0 53 43329.0 54 40472.0 55 37462.5 56 34530.5 57 30623.5 58 27302.5 59 24598.5 60 21456.5 61 18416.5 62 15259.5 63 11637.5 64 8351.0 65 6264.0 66 4813.5 67 3712.0 68 2808.0 69 2116.0 70 1653.5 71 1313.5 72 1053.5 73 952.0 74 823.5 75 608.5 76 437.5 77 293.5 78 197.5 79 148.0 80 106.0 81 78.5 82 49.0 83 31.5 84 26.5 85 19.5 86 14.0 87 10.0 88 8.5 89 7.5 90 5.5 91 5.0 92 5.5 93 6.5 94 4.5 95 2.5 96 4.0 97 3.0 98 2.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1554659326587903 2 0.09198811432573971 3 0.085093109920598 4 0.0990472855024324 5 0.11913035388883719 6 0.03792252422827937 7 0.041643637716768545 8 0.04624030732019634 9 0.045911973777094356 10-11 0.04427030606158443 12-13 0.0486754477648694 14-15 0.04659600199189016 16-17 0.04539211233384955 18-19 0.04966044839417536 20-21 0.051274754981093455 22-23 0.06495531927700954 24-25 0.0799765788739254 26-27 0.0820286635183128 28-29 0.07198712932511041 30-31 0.06096059450260205 32-33 0.050399198866154835 34-35 0.29216213110358374 36-37 0.4616096004728003 38-39 0.44015847565680394 40-41 0.41586179346725693 42-43 0.444700423003048 44-45 0.4546051515532913 46-47 0.4540305678528628 48-49 0.46869613277808486 50-51 0.4586272374562906 52-53 0.4656590475043914 54-55 0.456438347168944 56-57 0.43561652831055975 58-59 0.41567026556711417 60-61 0.38436913445805815 62-63 0.4410066706431507 64-65 0.4791480839001648 66-67 0.4818568356307561 68-69 0.49047559113718325 70-71 0.48732906134912257 72-73 0.47808099988508324 74-75 0.4708029396796559 76-77 0.45331917850947523 78-79 0.4282837458479487 80-81 0.43298985996574385 82-83 0.4452476455748847 84-85 0.44379750575951754 86-87 0.45427681801018926 88-89 0.465084463803963 90-91 0.46806682682047274 92-93 0.4714869678944517 94-95 0.4750986368685735 96-97 0.4769318324842263 98-99 0.4707208562938804 100-101 0.45753279231261734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1827410.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.46515243085338 #Duplication Level Percentage of deduplicated Percentage of total 1 68.46626190161255 23.597001528098062 2 14.131205408382305 9.740682968631917 3 6.161391947594148 6.370599379801946 4 3.2885054613641884 4.533553679824423 5 2.0255812352606832 3.490598301716786 6 1.3303879148233935 2.751121336593206 7 0.8620300954073293 2.0796999046737685 8 0.6471721969279602 1.7843910732905928 9 0.45478675493994086 1.4106865349284399 >10 2.3226389724427277 14.30993005456317 >50 0.18402065488615707 4.4264969126639135 >100 0.10671616916924614 6.746973007711676 >500 0.009411371439458428 2.1381658217955746 >1k 0.007337679427374368 5.593140955625696 >5k 0.0014356329314428112 3.4864219827982144 >10k+ 0.0011166033911221864 7.540536557282665 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26686 1.4603181552032658 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 24368 1.333471963051532 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 21162 1.1580324065207042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19380 1.0605173442194142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15706 0.8594677713266317 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15117 0.8272363618454533 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 14739 0.8065513486300283 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9418 0.5153742181557505 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 8391 0.4591744600281273 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8089 0.44264833835866063 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7484 0.4095413727625437 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6513 0.3564060610372057 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6046 0.3308507669324344 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5940 0.325050207670966 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5821 0.31853825906610994 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5714 0.3126829775474579 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4962 0.2715318401453423 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4880 0.2670446150562818 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4857 0.26578600314105755 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4536 0.24822015858510132 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4429 0.24236487706644924 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4012 0.2195456958208612 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3739 0.20460651960972084 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3499 0.19147317788564142 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3381 0.18501595153796904 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3120 0.17073344241303265 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2836 0.15519232137287198 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2806 0.15355065365736206 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2773 0.15174481917030114 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2737 0.14977481791168923 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2702 0.147859538910261 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2677 0.14649148248066934 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2402 0.13144286175516168 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1947 0.10654423473659441 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1928 0.10550451185010479 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1852 0.1013456203041463 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.4722257183664314E-5 0.0 3 0.0 0.0 0.0 5.4722257183664314E-5 0.0 4 0.0 0.0 0.0 5.4722257183664314E-5 0.0 5 0.0 0.0 0.0 5.4722257183664314E-5 0.0 6 0.0 0.0 0.0 5.4722257183664314E-5 0.0 7 0.0 0.0 0.0 5.4722257183664314E-5 0.0 8 0.0 0.0 0.0 2.1888902873465726E-4 0.0 9 0.0 0.0 0.0 2.1888902873465726E-4 0.0 10-11 0.0 0.0 0.0 2.462501573264894E-4 0.0 12-13 0.0 0.0 0.0 3.009724145101537E-4 0.0 14-15 0.0 0.0 0.0 3.830558002856502E-4 0.0 16-17 0.0 0.0 0.0 5.745837004284752E-4 0.0 18-19 0.0 0.0 0.0 7.934727291631326E-4 0.0 20-21 0.0 0.0 0.0 0.0013680564295916078 0.0 22-23 0.0 0.0 0.0 0.002489862701856726 5.4722257183664314E-5 24-25 0.0 0.0 0.0 0.0042136138031421515 5.4722257183664314E-5 26-27 0.0 0.0 0.0 0.007442226976978347 5.4722257183664314E-5 28-29 0.0 0.0 0.0 0.0139541755818344 5.4722257183664314E-5 30-31 0.0 0.0 0.0 0.03119168659468866 5.4722257183664314E-5 32-33 0.0 0.0 0.0 0.05830656502919432 5.4722257183664314E-5 34-35 0.0 0.0 0.0 0.10120881466118714 5.4722257183664314E-5 36-37 0.0 0.0 0.0 0.16597260603805386 5.4722257183664314E-5 38-39 0.0 0.0 0.0 0.24614071281212208 5.4722257183664314E-5 40-41 0.0 0.0 0.0 0.33930535566731057 5.4722257183664314E-5 42-43 0.0 0.0 0.0 0.4365194455540902 5.4722257183664314E-5 44-45 0.0 0.0 0.0 0.5442949310773171 5.4722257183664314E-5 46-47 0.0 0.0 0.0 0.6836177978669264 5.4722257183664314E-5 48-49 0.0 0.0 0.0 0.8354994226801866 5.4722257183664314E-5 50-51 0.0 0.0 0.0 0.9925523007973033 5.4722257183664314E-5 52-53 0.0 0.0 0.0 1.14960517891442 5.4722257183664314E-5 54-55 0.0 0.0 0.0 1.3140182006227392 5.4722257183664314E-5 56-57 0.0 0.0 0.0 1.4978302625026676 5.4722257183664314E-5 58-59 0.0 0.0 0.0 1.734996525136669 5.4722257183664314E-5 60-61 0.0 0.0 0.0 2.04354797226676 5.4722257183664314E-5 62-63 0.0 0.0 0.0 2.323917456947264 5.4722257183664314E-5 64-65 0.0 0.0 0.0 2.565871917084836 5.4722257183664314E-5 66-67 0.0 0.0 0.0 2.844818623078565 8.208338577549647E-5 68-69 0.0 0.0 0.0 3.1625907705441034 1.0944451436732863E-4 70-71 0.0 0.0 0.0 3.5302969776897357 1.0944451436732863E-4 72-73 0.0 0.0 0.0 3.9206034770522216 1.0944451436732863E-4 74-75 0.0 0.0 0.0 4.303932888623789 1.0944451436732863E-4 76-77 0.0 0.0 0.0 4.668082149052484 1.0944451436732863E-4 78-79 0.0 0.0 0.0 5.089990751938537 1.0944451436732863E-4 80-81 0.0 0.0 0.0 5.56166924773313 1.0944451436732863E-4 82-83 0.0 0.0 0.0 6.045632890265457 1.0944451436732863E-4 84-85 0.0 0.0 0.0 6.614935892875709 1.0944451436732863E-4 86-87 0.0 0.0 0.0 7.212667108092875 1.0944451436732863E-4 88-89 0.0 0.0 0.0 7.756688427884273 1.0944451436732863E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 90 0.0 63.179344 2 TACCTGG 1480 0.0 27.854408 2 ATAACGC 265 1.8189894E-12 25.033327 3 TAACGCA 210 1.3023964E-9 24.820456 4 CATGGGG 4460 0.0 23.373524 4 GAGTACT 14350 0.0 22.638153 12-13 TATCACG 150 3.092041E-5 22.11277 2 AGTACTT 14755 0.0 21.711876 12-13 CATGGGT 1465 0.0 21.347288 4 GTACTTT 15700 0.0 20.706924 14-15 GTACATG 24220 0.0 20.298649 1 ACCTGGG 1920 0.0 19.99034 3 TACATGG 24440 0.0 19.67892 2 ATGGGAG 3835 0.0 19.275055 5 ACATGGG 24110 0.0 19.04421 3 ATGGGGG 2390 0.0 19.03311 5 GATATAG 850 0.0 18.963675 1 ACTTTTT 17570 0.0 18.96203 16-17 TACTTTT 16990 0.0 18.828108 14-15 GCTTATA 3205 0.0 18.786167 1 >>END_MODULE