FastQCFastQC Report
Thu 26 May 2016
SRR938695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938695_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827410
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247791.355962810754018No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA238971.307697779918026No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA214931.176145473648497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205681.1255273857536074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169020.9249155909182942No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA161790.8853513989745049No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT130620.7147821233330233No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG88120.48221253030244987No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA83660.4578064035985356No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82920.4537569565669444No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT71840.3931246956074444No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71050.38880163728993494No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC64300.35186411369096154No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG56630.309892142431091No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55470.30354436059778594No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54220.2967040784498279No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52670.2882221285863599No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50160.2744868420332602No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG48910.26764655988530217No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45920.25128460498738653No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44750.24488210089689777No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT39610.21675486070449435No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG36450.19946262743445642No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35960.19678123683245685No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA34990.19147317788564142No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG34360.18802567568307058No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT30600.1674501069820128No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA28620.15661510005964724No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG27660.1513617633700155No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.14430259219332278No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT26170.14320814704964951No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24950.13653203167324246No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23870.1306220278974067No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC19900.10889729179549199No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19640.10747451310871671No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT19490.10665367925096175No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18490.1011814535325953No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG18480.10112673127541164No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC18450.10096256450386067No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1152.5302143E-937.13582
TACCTGG14850.024.9239142
GTCTATA3650.023.4938491
CCGTATG1252.2723389E-422.7766239
ATGGGAT42950.021.985585
CATGGGG41900.021.7437924
GAGTACT153100.021.46399312-13
AGTACTT156200.021.053212-13
GTACCGT1150.003746305620.6309996
ATATAGG9800.020.3362712
GTACATG235300.019.9631711
TAACGCA2401.4296893E-719.7713744
GTACTTT166250.019.69514814-15
TACATGG236050.019.4388482
GCTTATG3950.019.2973381
CCCTATA3951.8189894E-1219.220782
CTTACTA8950.019.0865559
ACATGGG234350.018.9116743
GATATAG9100.018.8467141
TGGGATA13200.018.6929346
Produced by FastQC (version 0.11.5)