##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938695_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1827410 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87867309470781 31.0 31.0 34.0 30.0 34.0 2 32.06625059510455 33.0 31.0 34.0 30.0 34.0 3 32.12825912083222 34.0 31.0 34.0 30.0 34.0 4 35.790832380254024 37.0 35.0 37.0 35.0 37.0 5 35.718802020345734 37.0 35.0 37.0 33.0 37.0 6 35.78678949989329 37.0 35.0 37.0 33.0 37.0 7 35.727143881230816 37.0 35.0 37.0 33.0 37.0 8 35.721868108415734 37.0 35.0 37.0 33.0 37.0 9 37.31201427156467 39.0 37.0 39.0 34.0 39.0 10-11 37.22788728309465 39.0 37.0 39.0 33.0 39.0 12-13 37.147918365336736 39.0 37.0 39.0 33.0 39.0 14-15 38.18290421963325 40.0 37.0 41.0 33.0 41.0 16-17 38.12826815000465 40.0 37.0 41.0 33.0 41.0 18-19 38.25572531615784 40.0 37.5 41.0 33.5 41.0 20-21 38.35307320196343 40.0 38.0 41.0 34.0 41.0 22-23 38.2868009915673 40.0 37.5 41.0 33.5 41.0 24-25 38.18076512659994 40.0 37.0 41.0 33.0 41.0 26-27 37.90620112618406 40.0 37.0 41.0 33.0 41.0 28-29 37.691146486010254 40.0 36.5 41.0 33.0 41.0 30-31 37.39990013188064 39.0 36.0 41.0 32.0 41.0 32-33 37.081971478759556 39.0 35.0 41.0 31.5 41.0 34-35 36.969921911339 39.0 35.0 41.0 31.0 41.0 36-37 36.78524441696171 39.0 35.0 41.0 31.0 41.0 38-39 36.60959144362786 39.0 35.0 41.0 30.5 41.0 40-41 36.39412912263805 39.0 35.0 41.0 30.0 41.0 42-43 36.21401519089859 39.0 35.0 41.0 30.0 41.0 44-45 35.90117789658588 39.0 35.0 41.0 29.5 41.0 46-47 35.4664497841207 38.0 35.0 41.0 27.0 41.0 48-49 35.10311232837732 38.0 35.0 40.0 26.0 41.0 50-51 34.87473117691158 38.0 34.0 40.0 24.5 41.0 52-53 34.60181103310149 38.0 34.0 40.0 24.0 41.0 54-55 34.388375077295194 38.0 34.0 40.0 24.0 41.0 56-57 34.1940738531583 38.0 34.0 40.0 23.0 41.0 58-59 33.93973410455234 37.5 33.0 40.0 23.0 41.0 60-61 33.556078821939245 37.0 33.0 40.0 22.0 41.0 62-63 33.12260576444257 36.0 33.0 39.0 21.5 41.0 64-65 32.68649536776093 36.0 32.0 39.0 20.0 40.5 66-67 32.281082242080316 35.0 32.0 39.0 18.5 40.0 68-69 31.88794112979572 35.0 31.0 38.0 17.5 40.0 70-71 31.38804181874894 35.0 31.0 37.0 13.5 39.5 72-73 31.001010720090182 35.0 31.0 37.0 10.0 39.0 74-75 30.57938858822049 35.0 30.0 36.0 7.5 39.0 76-77 28.510290794074674 32.5 27.0 34.5 4.0 36.5 78-79 29.567606065414985 34.0 29.0 35.0 2.0 37.0 80-81 29.67273272007924 34.0 30.0 35.0 2.0 37.0 82-83 29.38413765931017 34.0 29.5 35.0 2.0 36.5 84-85 29.013555797549536 34.0 29.0 35.0 2.0 36.0 86-87 28.43776766024045 34.0 29.0 35.0 2.0 36.0 88-89 27.85140581478705 33.5 27.0 35.0 2.0 35.0 90-91 27.35544869514778 33.0 26.0 35.0 2.0 35.0 92-93 27.084516063718596 33.0 26.0 35.0 2.0 35.0 94-95 26.71659698699252 33.0 25.0 35.0 2.0 35.0 96-97 26.10934656152697 33.0 24.5 35.0 2.0 35.0 98-99 25.3244277419955 32.5 21.0 35.0 2.0 35.0 100-101 22.17512763966488 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.00912876178305666 1101 2 0.09451145767769731 1101 3 0.11083825723634533 1101 4 0.10125297101564001 1101 5 0.037300014380477364 1101 6 0.17361950437324936 1101 7 0.23397218634286077 1101 8 -2.895811709180407E-4 1101 9 0.23355640486148133 1101 10-11 0.06644510343835464 1101 12-13 0.1711999563973734 1101 14-15 0.22571872542982874 1101 16-17 0.24897663329521436 1101 18-19 0.13296208362373818 1101 20-21 0.15692502674249909 1101 22-23 0.09340376297840436 1101 24-25 0.17806330947944815 1101 26-27 0.15073551383054706 1101 28-29 0.19731064000341547 1101 30-31 0.20589692939959292 1101 32-33 0.3302639026787162 1101 34-35 0.13199127410460676 1101 36-37 0.13497658771498067 1101 38-39 0.16240787865147865 1101 40-41 0.41158071283707187 1101 42-43 0.3978453852897559 1101 44-45 0.3926517475633702 1101 46-47 0.4715920826897886 1101 48-49 0.576602165521571 1101 50-51 0.42926609940746374 1101 52-53 0.44457892660665976 1101 54-55 0.42001765879617636 1101 56-57 0.2375486471029049 1101 58-59 0.2488087251648352 1101 60-61 0.3289616053934239 1101 62-63 0.45714789567911396 1101 64-65 0.270122170814183 1101 66-67 0.23622339893030642 1101 68-69 0.1185593724027818 1101 70-71 0.2669921689717363 1101 72-73 0.43148474551292537 1101 74-75 0.4472637014732648 1101 76-77 0.3053444058584347 1101 78-79 0.3487719392448483 1101 80-81 0.3696551103864856 1101 82-83 0.44801222847162236 1101 84-85 0.4058908488408228 1101 86-87 0.6203128433403933 1101 88-89 0.6867591803201698 1101 90-91 0.6384318425840014 1101 92-93 0.7403326154955963 1101 94-95 0.8626224313153124 1101 96-97 0.6615559418661334 1101 98-99 0.8016715076297523 1101 100-101 1.4633401994861224 1102 1 0.06648456371314992 1102 2 0.07527807250107088 1102 3 0.12485211699311094 1102 4 0.21099808276408538 1102 5 0.09114759510618597 1102 6 0.15882371461919575 1102 7 0.0697992295154819 1102 8 0.13324446655344246 1102 9 0.13076495282128775 1102 10-11 0.15477560679389768 1102 12-13 0.1087406819180643 1102 14-15 0.24825153176897885 1102 16-17 0.295672693680757 1102 18-19 0.23953191273112395 1102 20-21 0.2216577959827788 1102 22-23 0.2773261597555674 1102 24-25 0.21594505921429175 1102 26-27 0.42505834057406844 1102 28-29 0.3679692400443173 1102 30-31 0.34888928608143743 1102 32-33 0.47075636637652707 1102 34-35 0.4696542356406894 1102 36-37 0.5204333556425382 1102 38-39 0.3990225593396133 1102 40-41 0.6195189194397202 1102 42-43 0.6352067996127673 1102 44-45 0.4421321255913355 1102 46-47 0.35613659895383876 1102 48-49 0.5461016637355911 1102 50-51 0.6278625255497943 1102 52-53 0.7333422500945233 1102 54-55 0.68282651876698 1102 56-57 0.6256836679591302 1102 58-59 0.5529433464947857 1102 60-61 0.5561203552566809 1102 62-63 0.602309109287738 1102 64-65 0.6797136551969842 1102 66-67 0.6991169204214884 1102 68-69 0.6570080689483042 1102 70-71 0.48180365440091677 1102 72-73 0.48448666228780013 1102 74-75 0.4854793410244653 1102 76-77 0.3919464824438137 1102 78-79 0.4632718893040604 1102 80-81 0.3773293442710717 1102 82-83 0.49916966560568454 1102 84-85 0.6599015694625656 1102 86-87 0.5947417347976653 1102 88-89 0.8558253827753539 1102 90-91 0.8802180104135751 1102 92-93 0.8198150387175822 1102 94-95 0.8926449284496663 1102 96-97 1.134164610635068 1102 98-99 1.1387308991131846 1102 100-101 1.500943707707325 1103 1 0.09131388744644298 1103 2 0.09232009026893451 1103 3 0.0057446390992552665 1103 4 0.19589134391488017 1103 5 0.09699490885797957 1103 6 0.11971656351379067 1103 7 0.10092520112196013 1103 8 0.18332165461422534 1103 9 0.20869205825800918 1103 10-11 0.25190231933034823 1103 12-13 0.1749128296754634 1103 14-15 0.21732776051712221 1103 16-17 0.22017403492740328 1103 18-19 0.18312335552413117 1103 20-21 0.23928630475595014 1103 22-23 0.15891876327602006 1103 24-25 0.11506656387401648 1103 26-27 0.1832881463831768 1103 28-29 0.19894781120580518 1103 30-31 0.293812234706202 1103 32-33 0.2576715996516299 1103 34-35 0.11466833939037002 1103 36-37 0.17553281087990058 1103 38-39 0.32537936770992815 1103 40-41 0.20409287056661896 1103 42-43 0.22875360750232687 1103 44-45 0.3043596853582713 1103 46-47 0.38898370877706867 1103 48-49 0.4111326913564426 1103 50-51 0.5308638575269526 1103 52-53 0.45837961866821786 1103 54-55 0.4692537689018579 1103 56-57 0.28621173924425847 1103 58-59 0.18388009501882152 1103 60-61 0.16946425893955563 1103 62-63 0.161010056063013 1103 64-65 0.1651044831877968 1103 66-67 0.19555649739383796 1103 68-69 0.08828964213305213 1103 70-71 0.15760091610222204 1103 72-73 0.07319794374786426 1103 74-75 0.19471303153128972 1103 76-77 0.23456092203146994 1103 78-79 0.10624152280138333 1103 80-81 0.3115449435806674 1103 82-83 0.36889199282964213 1103 84-85 0.3366444752466258 1103 86-87 0.42737341561835507 1103 88-89 0.6491558818473067 1103 90-91 0.795819865189415 1103 92-93 0.6375157865918091 1103 94-95 0.43748203226187243 1103 96-97 0.6841211383443735 1103 98-99 0.703894244635098 1103 100-101 1.032566303494832 1104 1 0.0016084066780273076 1104 2 0.026059141696229915 1104 3 0.041517161485494114 1104 4 0.011643368246680552 1104 5 0.0732211787858148 1104 6 -0.008198130970207274 1104 7 0.07798920126492703 1104 8 -0.03859909899182412 1104 9 -0.052937170249528265 1104 10-11 0.13666503506861005 1104 12-13 0.051491215922396805 1104 14-15 0.08761564562769308 1104 16-17 0.20749160835655545 1104 18-19 0.19339511307397572 1104 20-21 0.2028246614816709 1104 22-23 0.12110521405576691 1104 24-25 0.09008812633491914 1104 26-27 0.19855383571882612 1104 28-29 0.16262066877818881 1104 30-31 0.20666415115515946 1104 32-33 0.11604941911387101 1104 34-35 0.06077284686174522 1104 36-37 0.022745649220858866 1104 38-39 0.13185663377485923 1104 40-41 0.3048620649190923 1104 42-43 0.20929628863405725 1104 44-45 0.15088324605028447 1104 46-47 0.07574346424261336 1104 48-49 -0.026782627035906614 1104 50-51 5.1876856197452526E-5 1104 52-53 -0.04458378926344864 1104 54-55 7.043934529349372E-4 1104 56-57 0.15288691280208155 1104 58-59 0.04171975433349928 1104 60-61 0.1679187532900528 1104 62-63 -0.2211615588657665 1104 64-65 0.20212623313474154 1104 66-67 -0.008882438124452108 1104 68-69 0.08403154598156348 1104 70-71 0.03612501855988626 1104 72-73 -0.13855247660647052 1104 74-75 -0.20967316962240545 1104 76-77 -0.11516667456380958 1104 78-79 -0.022479549719022884 1104 80-81 -0.135932162274635 1104 82-83 -0.0142868108401899 1104 84-85 0.09969897379831139 1104 86-87 0.30444653924532616 1104 88-89 0.3374605159187887 1104 90-91 0.33265632376017606 1104 92-93 0.45212204555265245 1104 94-95 0.5722112795493608 1104 96-97 0.5885512325482622 1104 98-99 0.8618629808695317 1104 100-101 1.2980005891714939 1105 1 -0.21125417074629738 1105 2 -0.12658822897939714 1105 3 -0.10566889371339983 1105 4 -0.18892247257078765 1105 5 -0.09627792552026193 1105 6 -0.11167518491478035 1105 7 -0.0926382587367982 1105 8 -0.11647011598706314 1105 9 -0.015530823926241055 1105 10-11 -0.015454958661372586 1105 12-13 -0.15063781635554108 1105 14-15 -0.025851544443270313 1105 16-17 -0.14926419040317995 1105 18-19 -0.24640184449228286 1105 20-21 -0.13920061380909488 1105 22-23 -0.12230272810246134 1105 24-25 -0.1717435176984239 1105 26-27 -0.14241360610726161 1105 28-29 -0.1181645177346411 1105 30-31 -0.2912777699381621 1105 32-33 -0.27688135854308626 1105 34-35 -0.24101857178377628 1105 36-37 -0.2213876864529496 1105 38-39 -0.29357242009088225 1105 40-41 -0.48214247406415467 1105 42-43 -0.3871710403256756 1105 44-45 -0.2263697313582682 1105 46-47 -0.3425500640646675 1105 48-49 -0.3114396072123853 1105 50-51 -0.24722370154157858 1105 52-53 -0.4329457118164797 1105 54-55 -0.4390553012751113 1105 56-57 -0.503135208389601 1105 58-59 -0.38733426538746585 1105 60-61 -0.42750506222132856 1105 62-63 -0.5011884004275657 1105 64-65 -0.5964662786361643 1105 66-67 -0.4188310923970633 1105 68-69 -0.13092652716631292 1105 70-71 -0.34071483407636904 1105 72-73 -0.3824796348408057 1105 74-75 -0.32772338083494645 1105 76-77 -0.37772417404875114 1105 78-79 -0.6049519113176061 1105 80-81 -0.32459853145451234 1105 82-83 -0.45984457239682897 1105 84-85 -0.35944802132333464 1105 86-87 -0.33148259730695884 1105 88-89 -0.06825264707102363 1105 90-91 0.01153365295702713 1105 92-93 0.05909811080019267 1105 94-95 0.044668498885506835 1105 96-97 -0.03318122458484396 1105 98-99 0.024915768145227446 1105 100-101 0.22695544662882128 1106 1 -0.02341042769445778 1106 2 -0.12133847392113495 1106 3 -0.040984728066810305 1106 4 -0.04460750314880357 1106 5 0.031893184304045974 1106 6 -0.09232173337415617 1106 7 0.0012767409822203035 1106 8 -0.11652356337406644 1106 9 -0.06294415364347827 1106 10-11 -0.16085292485086455 1106 12-13 -0.0342961403432156 1106 14-15 -0.0367566438737299 1106 16-17 -0.036164938300096594 1106 18-19 -0.08615023862735427 1106 20-21 -0.19573634354001967 1106 22-23 -0.17459076492801273 1106 24-25 -0.289795357388023 1106 26-27 -0.1067777898831963 1106 28-29 -0.14336373905202038 1106 30-31 -0.0748169215234995 1106 32-33 -0.18868981043799238 1106 34-35 -0.17985185502927692 1106 36-37 -0.34401813472687337 1106 38-39 -0.2142966596720015 1106 40-41 -0.09850736151311423 1106 42-43 -0.060516187058603066 1106 44-45 -0.10126619613430421 1106 46-47 -0.22044278935609185 1106 48-49 -0.3088718420339376 1106 50-51 -0.28507147605346717 1106 52-53 -0.27537899726095816 1106 54-55 -0.22845335542373135 1106 56-57 -0.3694474119856608 1106 58-59 -0.3958534734621182 1106 60-61 -0.3935452033468678 1106 62-63 -0.38562354400862375 1106 64-65 -0.39862665256018914 1106 66-67 -0.3479687142265675 1106 68-69 -0.5222572999116899 1106 70-71 -0.3433171109961357 1106 72-73 -0.2203283561349778 1106 74-75 -0.26905311517394637 1106 76-77 -0.423362592397023 1106 78-79 -0.46822983040009447 1106 80-81 -0.4056133171396503 1106 82-83 -0.11274762066928545 1106 84-85 -0.1660307847548168 1106 86-87 -0.08754230933854501 1106 88-89 0.04601985153018262 1106 90-91 0.05560163436833321 1106 92-93 0.20299420541489255 1106 94-95 0.29195398245303394 1106 96-97 0.3037895852010948 1106 98-99 0.09266216365344704 1106 100-101 0.6527869658051344 1107 1 0.04107396394102736 1107 2 -0.12373428397230057 1107 3 -0.06978852339042874 1107 4 -0.03141357441686665 1107 5 -0.0395503220443274 1107 6 -0.07099466790049291 1107 7 0.01892702661685064 1107 8 0.022817650446945947 1107 9 -0.11016669630842557 1107 10-11 0.07427683041901645 1107 12-13 0.023657979068161694 1107 14-15 -0.07185052958623572 1107 16-17 0.037992818056082456 1107 18-19 0.009673000760180628 1107 20-21 0.010656008133054229 1107 22-23 -0.10466255210918263 1107 24-25 0.020901401253716756 1107 26-27 -0.021429122163880265 1107 28-29 0.003269299153558336 1107 30-31 -0.0364501640113275 1107 32-33 0.0736233013572658 1107 34-35 0.06591259313230324 1107 36-37 0.19690906028575483 1107 38-39 0.20562785804167305 1107 40-41 0.16792429045724333 1107 42-43 0.19980937874311167 1107 44-45 0.21471636537267358 1107 46-47 0.09869398000445528 1107 48-49 -0.12459199099173901 1107 50-51 0.06884299217583845 1107 52-53 0.1602908742335032 1107 54-55 -0.034313431705122355 1107 56-57 -0.057110987982262884 1107 58-59 0.13427274892673324 1107 60-61 -0.10856036829876103 1107 62-63 -0.12471547341829847 1107 64-65 0.12258625519316269 1107 66-67 -0.03408635435817331 1107 68-69 0.07824295123529268 1107 70-71 0.08763834706472196 1107 72-73 0.2494395251698336 1107 74-75 0.12610833989237236 1107 76-77 0.050813278986879595 1107 78-79 -0.0932175133829034 1107 80-81 -0.004806913011709213 1107 82-83 0.17341078578506952 1107 84-85 0.06288909094545048 1107 86-87 0.12535012284575942 1107 88-89 0.17460545540048855 1107 90-91 0.20868097879058567 1107 92-93 0.1861894379481619 1107 94-95 0.2584883463301644 1107 96-97 0.26081641346702966 1107 98-99 0.3150800061446333 1107 100-101 1.0342197533443986 1108 1 -0.11664665078480851 1108 2 -0.07584201833616078 1108 3 -0.1343126784806259 1108 4 -0.12245134702830285 1108 5 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-0.942724672122452 2316 62-63 -1.0477707863696182 2316 64-65 -0.9320624905633039 2316 66-67 -1.1135932683751548 2316 68-69 -1.1454895333501618 2316 70-71 -1.0082457802572442 2316 72-73 -1.2067979317832034 2316 74-75 -1.1040362359253244 2316 76-77 -1.0806827043839249 2316 78-79 -0.9035756135511335 2316 80-81 -1.0686888907377856 2316 82-83 -1.2188639612745256 2316 84-85 -1.3363893755021792 2316 86-87 -1.849895274401355 2316 88-89 -2.302343064387454 2316 90-91 -2.7592071900326083 2316 92-93 -2.753947659950601 2316 94-95 -2.653968219356056 2316 96-97 -2.8528104424554073 2316 98-99 -2.980752827709381 2316 100-101 -3.7907997977032046 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 18.0 4 43.0 5 143.0 6 432.0 7 744.0 8 1240.0 9 2076.0 10 3253.0 11 4731.0 12 6492.0 13 8927.0 14 10314.0 15 13279.0 16 17272.0 17 23248.0 18 21363.0 19 14265.0 20 12453.0 21 13128.0 22 14181.0 23 15747.0 24 17748.0 25 20716.0 26 24766.0 27 30054.0 28 36074.0 29 43069.0 30 52049.0 31 62308.0 32 75617.0 33 94878.0 34 129099.0 35 197423.0 36 242516.0 37 341771.0 38 253506.0 39 22462.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.93497386288299 20.04409557116343 14.980300353017235 19.04063021293635 2 14.775173606360914 23.78842186482508 39.47225855172074 21.964145977093263 3 20.69863905746384 29.967713868261637 30.03491280008318 19.298734274191343 4 12.084480220640142 18.261145555731883 37.909062553012184 31.745311670615788 5 11.710344148275428 41.33992918939921 32.84446292840687 14.105263733918497 6 26.660738422138436 37.94561702081087 18.649181081421247 16.74446347562944 7 25.4344673609097 36.833551310324445 20.833693588193125 16.898287740572723 8 25.141046617890893 33.633612599252494 20.347595777630637 20.877745005225975 9 27.333384407440036 18.679661378672545 21.10763320765455 32.879321006232864 10-11 23.722974045233418 28.016564427249495 26.33002446084896 21.930437066668127 12-13 28.205657186947647 25.9838514619051 26.718990264910445 19.091501086236807 14-15 24.704957867931554 26.364302234557847 28.45110986405065 20.47963003345995 16-17 19.316229821553556 30.185544026621674 29.784369231846853 20.713856919977914 18-19 19.048499815859504 29.478868715213725 32.832141025900604 18.640490443026163 20-21 20.736588942820713 27.795760119513407 31.581637399379446 19.886013538286427 22-23 19.98101137675727 27.609485556060214 30.903738077388216 21.505764989794297 24-25 20.417448738925582 28.008492894314905 31.949890829096915 19.624167537662593 26-27 21.209713430169945 26.53704044198121 33.00198970344882 19.251256424400022 28-29 19.485473976830598 27.01402531451617 34.10575623423315 19.39474447442008 30-31 19.434418110878237 29.06531648617442 32.703553116158936 18.796712286788406 32-33 19.116700685669883 28.903639577325286 31.94236651873417 20.037293218270666 34-35 19.89123796306228 27.50053084017223 32.166013970399916 20.44221722636557 36-37 21.047231971872844 27.332906069679737 32.68758507189687 18.932276886550554 38-39 21.627071430135068 26.633419802543727 32.951582714914295 18.78792605240691 40-41 19.613692943106052 27.638010000673134 33.76026185411407 18.98803520210674 42-43 19.12644994047422 29.151871867577235 32.12780517462492 19.59387301732363 44-45 19.44331350170517 29.30374787127391 30.8090965007902 20.44384212623072 46-47 19.603570454307285 28.732837853404302 29.949903865438426 21.713687826849984 48-49 20.41635429378191 28.546029626630038 30.234430149774816 20.803185929813235 50-51 21.159442575369212 29.053372981562912 30.36602743564094 19.421157007426928 52-53 19.767567212612384 29.3940057239481 30.93525809752601 19.903168965913505 54-55 18.672191790566977 29.089749974006928 31.370464208907688 20.867594026518404 56-57 20.05852545405793 30.042601277217486 29.28138731866412 20.61748595006047 58-59 19.82888279949272 28.814752086636634 30.050905588670258 21.305459525200387 60-61 19.878291595082743 28.342090308063284 30.856454291218594 20.92316380563538 62-63 21.589462682156714 27.937545487876285 30.33955160582464 20.133440224142365 64-65 20.67275542981597 28.451305399445115 31.118741825862834 19.757197344876083 66-67 19.546117935351546 28.771319329032487 30.97725117394143 20.70531156167454 68-69 19.217101227371554 30.071369119312074 30.381974180817455 20.32955547249892 70-71 19.587974974129754 29.790236233226754 29.14453353938324 21.477255253260253 72-73 19.687645355995645 28.931137511560078 28.780897554462324 22.600319577981953 74-75 19.902074520769833 29.531002894807408 29.258595498547123 21.30832708587564 76-77 20.62618500723979 28.10207953279733 30.392548069220442 20.879187390742434 78-79 19.647648595644394 27.982772471537213 31.243439429252582 21.126139503565803 80-81 19.251755216399165 30.07376560268358 29.964321088316254 20.710158092601002 82-83 19.305930251010995 29.559677357571644 29.253396884114675 21.880995507302686 84-85 19.437509918409116 28.463125406996788 29.172736277025958 22.926628397568145 86-87 20.226909660854865 28.349267153860698 29.91157620147583 21.51224698380861 88-89 20.929383530189398 27.165560852447296 30.25000981329486 21.65504580406844 90-91 19.62544333842096 27.80967567669898 31.008543978814686 21.55633700606537 92-93 19.056259952610525 29.300731636578547 30.107638679880267 21.53536973093066 94-95 18.98483043478499 29.2756027796733 29.284741401623833 22.454825383917886 96-97 19.111370494681132 28.27319218817676 28.751027068645534 23.864410248496572 98-99 19.874992270468482 27.968974618133778 28.859170398274713 23.29686271312302 100-101 20.57456995630134 27.732766991818714 29.556777752453417 22.135885299426537 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15078.0 1 9460.0 2 6691.0 3 8503.5 4 5839.5 5 4809.0 6 8019.5 7 15476.0 8 20685.5 9 19823.5 10 14351.5 11 8887.0 12 7500.5 13 7107.0 14 6651.5 15 6129.5 16 5665.0 17 5446.5 18 5446.5 19 5462.5 20 5575.0 21 6029.0 22 6607.0 23 7186.5 24 7830.5 25 8722.5 26 10525.5 27 12585.5 28 13637.0 29 15311.5 30 18197.5 31 20908.0 32 24801.0 33 29667.5 34 34054.5 35 37281.5 36 42531.0 37 47628.5 38 48511.0 39 48676.5 40 47847.5 41 46508.5 42 45216.0 43 45932.5 44 50417.5 45 53191.5 46 55874.0 47 68051.5 48 128407.5 49 128875.5 50 76013.0 51 64370.0 52 51011.5 53 45134.5 54 41159.5 55 37298.0 56 33853.5 57 30486.5 58 27602.0 59 24517.5 60 21342.0 61 18309.5 62 15084.5 63 11444.5 64 8144.0 65 6023.0 66 4638.5 67 3512.0 68 2566.0 69 1888.0 70 1478.0 71 1155.0 72 977.0 73 952.5 74 797.0 75 566.0 76 367.0 77 226.0 78 157.0 79 105.0 80 54.0 81 30.5 82 21.5 83 13.5 84 9.0 85 8.5 86 6.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37315107173540696 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.208338577549646E-5 16-17 0.0014775009439589365 18-19 5.472225718366431E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.0944451436732861E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006457226347672388 36-37 0.015267509754242342 38-39 0.009302783721222932 40-41 0.007278060205427353 42-43 0.0027634739877750478 44-45 0.001422778686775272 46-47 0.0012859730438161113 48-49 0.0 50-51 3.2833354310198584E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.1041692887748235E-4 60-61 5.198614432448109E-4 62-63 0.0 64-65 0.0 66-67 0.0010397228864896218 68-69 4.925003146529788E-4 70-71 0.0011491674008569507 72-73 0.0 74-75 0.0 76-77 0.09497047734224941 78-79 0.03734794052785089 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14638203796630203 88-89 0.32294340076939493 90-91 0.2744594809046684 92-93 0.0 94-95 5.472225718366431E-5 96-97 2.7361128591832154E-5 98-99 1.6416677155099292E-4 100-101 4.651391860611467E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1827410.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.35342070616926 #Duplication Level Percentage of deduplicated Percentage of total 1 67.22922588543646 22.423246547070466 2 14.658914741043883 9.778499009078056 3 6.308305437047146 6.312106955545451 4 3.4792787781619294 4.641833953683254 5 2.056696331449701 3.429892900383839 6 1.3965080579702522 2.794699246622234 7 0.9493782540891464 2.2165508622546626 8 0.6492678167189653 1.7324242113602912 9 0.47763325303365606 1.433763254851895 >10 2.4611812133359035 14.687297184289172 >50 0.1985907075645965 4.597377212143915 >100 0.11446246352769425 6.928914541030608 >500 0.010031845582893655 2.2143697217888154 >1k 0.007564998308411609 5.291010734796858 >5k 0.0018090213346201671 4.009890615349259 >10k+ 0.0011511953947582882 7.50812304975113 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24779 1.355962810754018 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 23897 1.307697779918026 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 21493 1.176145473648497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20568 1.1255273857536074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16902 0.9249155909182942 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16179 0.8853513989745049 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13062 0.7147821233330233 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8812 0.48221253030244987 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 8366 0.4578064035985356 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8292 0.4537569565669444 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7184 0.3931246956074444 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7105 0.38880163728993494 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6430 0.35186411369096154 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5663 0.309892142431091 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5547 0.30354436059778594 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5422 0.2967040784498279 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5267 0.2882221285863599 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5016 0.2744868420332602 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4891 0.26764655988530217 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4592 0.25128460498738653 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4475 0.24488210089689777 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3961 0.21675486070449435 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3645 0.19946262743445642 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3596 0.19678123683245685 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3499 0.19147317788564142 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3436 0.18802567568307058 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3060 0.1674501069820128 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2862 0.15661510005964724 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2766 0.1513617633700155 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2637 0.14430259219332278 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2617 0.14320814704964951 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2495 0.13653203167324246 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2387 0.1306220278974067 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1990 0.10889729179549199 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1964 0.10747451310871671 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1949 0.10665367925096175 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1849 0.1011814535325953 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1848 0.10112673127541164 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1845 0.10096256450386067 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.4722257183664314E-5 0.0 3 0.0 0.0 0.0 5.4722257183664314E-5 0.0 4 0.0 0.0 0.0 5.4722257183664314E-5 0.0 5 0.0 0.0 0.0 5.4722257183664314E-5 0.0 6 0.0 0.0 0.0 5.4722257183664314E-5 0.0 7 0.0 0.0 0.0 5.4722257183664314E-5 0.0 8 0.0 0.0 0.0 1.0944451436732863E-4 0.0 9 0.0 0.0 0.0 1.6416677155099292E-4 0.0 10-11 0.0 0.0 0.0 1.915279001428251E-4 0.0 12-13 0.0 0.0 0.0 2.462501573264894E-4 0.0 14-15 0.0 0.0 0.0 3.830558002856502E-4 0.0 16-17 0.0 0.0 0.0 5.745837004284752E-4 0.0 18-19 0.0 0.0 0.0 7.934727291631326E-4 0.0 20-21 0.0 0.0 0.0 0.0012859730438161115 0.0 22-23 0.0 0.0 0.0 0.002435140444673062 0.0 24-25 0.0 0.0 0.0 0.0042136138031421515 0.0 26-27 0.0 0.0 0.0 0.007387504719794683 0.0 28-29 0.0 0.0 0.0 0.013872092196058904 0.0 30-31 0.0 0.0 0.0 0.03154738126638248 0.0 32-33 0.0 0.0 0.0 0.059017954372581956 0.0 34-35 0.0 0.0 0.0 0.10265895447655425 0.0 36-37 0.0 0.0 0.0 0.16816149632540045 0.0 38-39 0.0 0.0 0.0 0.2489589090570808 0.0 40-41 0.0 0.0 0.0 0.3426981356126978 0.0 42-43 0.0 0.0 0.0 0.4401584756568039 0.0 44-45 0.0 0.0 0.0 0.5480434056943981 0.0 46-47 0.0 0.0 0.0 0.6884333564990889 0.0 48-49 0.0 0.0 0.0 0.8411084540415124 0.0 50-51 0.0 0.0 0.0 0.9993925829452615 0.0 52-53 0.0 0.0 0.0 1.1568011557340716 0.0 54-55 0.0 0.0 0.0 1.3226643172577583 0.0 56-57 0.0 0.0 0.0 1.5071056850952989 0.0 58-59 0.0 0.0 0.0 1.745229587230014 0.0 60-61 0.0 0.0 0.0 2.054492423703493 0.0 62-63 0.0 0.0 0.0 2.335710103370344 0.0 64-65 0.0 0.0 0.0 2.578895814294548 0.0 66-67 0.0 0.0 0.0 2.8583897428601137 0.0 68-69 0.0 0.0 0.0 3.1770921686977744 0.0 70-71 0.0 0.0 0.0 3.5464674046875087 0.0 72-73 0.0 0.0 0.0 3.937923071450851 0.0 74-75 0.0 0.0 0.0 4.3238244291100525 0.0 76-77 0.0 0.0 0.0 4.689423829354114 0.0 78-79 0.0 0.0 0.0 5.111934377069185 0.0 80-81 0.0 0.0 0.0 5.584050650921249 0.0 82-83 0.0 0.0 0.0 6.067713321039067 0.0 84-85 0.0 0.0 0.0 6.635265211419441 0.0 86-87 0.0 0.0 0.0 7.232503926321953 0.0 88-89 0.0 0.0 0.0 7.775540245484046 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 115 2.5302143E-9 37.1358 2 TACCTGG 1485 0.0 24.923914 2 GTCTATA 365 0.0 23.493849 1 CCGTATG 125 2.2723389E-4 22.776623 9 ATGGGAT 4295 0.0 21.98558 5 CATGGGG 4190 0.0 21.743792 4 GAGTACT 15310 0.0 21.463993 12-13 AGTACTT 15620 0.0 21.0532 12-13 GTACCGT 115 0.0037463056 20.630999 6 ATATAGG 980 0.0 20.336271 2 GTACATG 23530 0.0 19.963171 1 TAACGCA 240 1.4296893E-7 19.771374 4 GTACTTT 16625 0.0 19.695148 14-15 TACATGG 23605 0.0 19.438848 2 GCTTATG 395 0.0 19.297338 1 CCCTATA 395 1.8189894E-12 19.22078 2 CTTACTA 895 0.0 19.086555 9 ACATGGG 23435 0.0 18.911674 3 GATATAG 910 0.0 18.846714 1 TGGGATA 1320 0.0 18.692934 6 >>END_MODULE