##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938694_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1822841 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74672886993435 33.0 31.0 34.0 30.0 34.0 2 31.764200498013814 33.0 31.0 34.0 30.0 34.0 3 31.75785655468579 33.0 31.0 34.0 30.0 34.0 4 35.32402167824841 37.0 35.0 37.0 33.0 37.0 5 35.379411040238836 37.0 35.0 37.0 33.0 37.0 6 35.48416126255663 37.0 35.0 37.0 33.0 37.0 7 35.425218655933236 37.0 35.0 37.0 33.0 37.0 8 35.41268821581257 37.0 35.0 37.0 33.0 37.0 9 36.97294662562451 39.0 37.0 39.0 33.0 39.0 10-11 36.863271947471006 39.0 37.0 39.0 33.0 39.0 12-13 36.748856592538786 39.0 37.0 39.0 32.5 39.0 14-15 37.802904641710384 40.0 38.0 41.0 32.5 41.0 16-17 37.72280357968687 40.0 37.5 41.0 32.5 41.0 18-19 37.78295117347042 40.0 37.5 41.0 33.0 41.0 20-21 37.70908022147845 40.0 37.5 41.0 32.5 41.0 22-23 37.7086849593574 40.0 37.0 41.0 32.5 41.0 24-25 37.62971729295095 40.0 37.0 41.0 32.0 41.0 26-27 37.418808332707016 40.0 37.0 41.0 32.0 41.0 28-29 37.21756395648332 40.0 36.0 41.0 31.5 41.0 30-31 36.948490296191494 39.0 36.0 41.0 31.0 41.0 32-33 36.68219608841363 39.0 35.0 41.0 31.0 41.0 34-35 36.38591051002254 39.0 35.0 41.0 30.0 41.0 36-37 36.11682834652062 39.0 35.0 41.0 30.0 41.0 38-39 35.89864831875079 38.0 35.0 40.0 30.0 41.0 40-41 35.741426706992 38.0 35.0 40.0 30.0 41.0 42-43 35.70417441784555 39.0 35.0 41.0 29.5 41.0 44-45 35.46529017067314 39.0 35.0 41.0 27.5 41.0 46-47 35.02821419970255 38.0 35.0 41.0 24.5 41.0 48-49 34.6816442026485 38.0 35.0 40.0 23.5 41.0 50-51 33.518889744086295 37.0 32.5 39.5 21.5 40.5 52-53 33.58515964914109 37.5 33.5 39.5 22.0 40.5 54-55 34.09757845034207 38.0 34.0 40.0 22.0 41.0 56-57 34.04617407661996 38.0 34.0 40.0 22.0 41.0 58-59 33.873346057061475 38.0 34.0 40.0 21.0 41.0 60-61 33.53892961591274 37.0 33.5 40.0 19.5 41.0 62-63 33.105009707374364 36.5 33.0 40.0 17.5 41.0 64-65 32.656987087738315 36.0 33.0 39.0 13.0 41.0 66-67 32.26222501029986 35.5 32.0 39.0 10.0 41.0 68-69 31.88071532294918 35.0 32.0 38.5 7.5 40.0 70-71 31.41268080979087 35.0 31.0 37.5 4.0 40.0 72-73 30.935679798731762 35.0 31.0 37.0 2.0 39.0 74-75 30.479127362178048 35.0 30.5 36.5 2.0 39.0 76-77 29.975424077031402 35.0 30.0 36.0 2.0 39.0 78-79 29.551718443901578 34.0 29.5 35.5 2.0 37.5 80-81 29.13034845057797 34.0 29.0 35.0 2.0 37.0 82-83 28.74313886948999 34.0 29.0 35.0 2.0 37.0 84-85 28.415241647516158 34.0 29.0 35.0 2.0 36.0 86-87 27.960208816896262 34.0 27.5 35.0 2.0 36.0 88-89 27.44671751403441 34.0 26.5 35.0 2.0 35.5 90-91 26.946608069491525 33.5 25.5 35.0 2.0 35.0 92-93 26.518746286703006 33.0 25.0 35.0 2.0 35.0 94-95 26.028273722173246 33.0 23.5 35.0 2.0 35.0 96-97 25.383195243029974 33.0 19.5 35.0 2.0 35.0 98-99 24.62398201488775 32.5 6.0 35.0 2.0 35.0 100-101 22.245055657624555 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11452872151947702 1101 2 0.13296287780729799 1101 3 0.270484621144778 1101 4 0.08053517871331906 1101 5 0.14460489750700134 1101 6 0.20635651602449911 1101 7 0.315864855185815 1101 8 0.28111375192020915 1101 9 0.3817017850771336 1101 10-11 0.30301486488140483 1101 12-13 0.2306298935146529 1101 14-15 0.2767155564745707 1101 16-17 0.48452729970650665 1101 18-19 0.47184699186318113 1101 20-21 0.5325008049745676 1101 22-23 0.44015880545681085 1101 24-25 0.43069720731337924 1101 26-27 0.3938212334768565 1101 28-29 0.5200548242258662 1101 30-31 0.5348902158816102 1101 32-33 0.4475381663848097 1101 34-35 0.5348935995311237 1101 36-37 0.522806113719291 1101 38-39 0.4350331200566515 1101 40-41 0.7436087767283581 1101 42-43 0.6905301027710422 1101 44-45 0.7632872300685278 1101 46-47 0.7297240373749858 1101 48-49 0.8455673380771103 1101 50-51 0.7679853721509033 1101 52-53 0.8453679714336388 1101 54-55 0.6518811716485473 1101 56-57 0.5654050048577304 1101 58-59 0.5580172079259 1101 60-61 0.4474242669147017 1101 62-63 0.42994641313602955 1101 64-65 0.4256429362912826 1101 66-67 0.4940678081396186 1101 68-69 0.6605223664821871 1101 70-71 0.43009701547509493 1101 72-73 0.38470845993653313 1101 74-75 0.4119534698131986 1101 76-77 0.4228871751723844 1101 78-79 0.33256733768260816 1101 80-81 0.4257400148363111 1101 82-83 0.5086236035548737 1101 84-85 0.39286542943179725 1101 86-87 0.3816409000589651 1101 88-89 0.44510379294746016 1101 90-91 0.41854631486172167 1101 92-93 0.41914258699370777 1101 94-95 0.4477897388822676 1101 96-97 0.33420497331219323 1101 98-99 0.3281126673506165 1101 100-101 0.7178379023877177 1102 1 0.06705708792935283 1102 2 0.1508492160855397 1102 3 0.07002382788112982 1102 4 0.2869456497515728 1102 5 0.20948653751911905 1102 6 0.24599318426660943 1102 7 0.21514702770303984 1102 8 0.15043141347301514 1102 9 0.2540186416445138 1102 10-11 0.3136002227792858 1102 12-13 0.2649769658619121 1102 14-15 0.37805955224214927 1102 16-17 0.447182882177124 1102 18-19 0.5546436425565489 1102 20-21 0.4901789507765173 1102 22-23 0.5158624277678854 1102 24-25 0.3662085025444881 1102 26-27 0.4053179421624691 1102 28-29 0.5233234781675407 1102 30-31 0.49349497358901573 1102 32-33 0.5222447290045622 1102 34-35 0.5409419350364644 1102 36-37 0.6452896864363566 1102 38-39 0.637603338925743 1102 40-41 0.7442626924999374 1102 42-43 0.7115702512262629 1102 44-45 0.7239266493197931 1102 46-47 0.6089279743117331 1102 48-49 0.4063275513874558 1102 50-51 0.6785035811307907 1102 52-53 0.6616483160081046 1102 54-55 0.537632128088994 1102 56-57 0.45017061721239315 1102 58-59 0.35849104250833363 1102 60-61 0.378680485049415 1102 62-63 0.31822331578890584 1102 64-65 0.33431177527786105 1102 66-67 0.23203595834107915 1102 68-69 0.24850764304909134 1102 70-71 0.27577350999327166 1102 72-73 0.24359507044844975 1102 74-75 0.14158103379659082 1102 76-77 0.23901429195878166 1102 78-79 0.2428392106410442 1102 80-81 0.18213434608642487 1102 82-83 0.12456046507364249 1102 84-85 -0.19374125950068688 1102 86-87 -0.1643305194130491 1102 88-89 -0.01591515026175827 1102 90-91 -0.12149731651970086 1102 92-93 0.037079271545170656 1102 94-95 0.06424347441850387 1102 96-97 -0.1212524486318145 1102 98-99 -0.29581670357994483 1102 100-101 -0.2575730455989991 1103 1 0.08396840488061486 1103 2 0.23084459087129972 1103 3 0.2549167671534569 1103 4 0.2205225012184897 1103 5 0.11429679052015018 1103 6 0.17914132788614978 1103 7 0.1569508431424964 1103 8 0.18822170831231233 1103 9 0.24287072807805288 1103 10-11 0.38342374911870536 1103 12-13 0.33702962205421727 1103 14-15 0.35019278739535764 1103 16-17 0.35511953244118644 1103 18-19 0.3292885320721908 1103 20-21 0.3874199440624082 1103 22-23 0.44667909508365256 1103 24-25 0.35764687473474766 1103 26-27 0.388984626037832 1103 28-29 0.36920466752386716 1103 30-31 0.4547566909745413 1103 32-33 0.4663283946619572 1103 34-35 0.447911577388453 1103 36-37 0.6441798765441433 1103 38-39 0.7041852905065156 1103 40-41 0.7539498293625044 1103 42-43 0.7275092768940894 1103 44-45 0.7541144746811241 1103 46-47 0.8179011616549516 1103 48-49 0.7414158697326201 1103 50-51 0.6197686436451519 1103 52-53 0.723704380729707 1103 54-55 0.7306732336238824 1103 56-57 0.7180728682003092 1103 58-59 0.7061320473874488 1103 60-61 0.6817366285687214 1103 62-63 0.5797255926200577 1103 64-65 0.43635133776640345 1103 66-67 0.39628676571570054 1103 68-69 0.4976087906275417 1103 70-71 0.5405384950685033 1103 72-73 0.7034749597935175 1103 74-75 0.6465267222485629 1103 76-77 0.7063118837377367 1103 78-79 0.5440265502341184 1103 80-81 0.6405836494210781 1103 82-83 0.48789489432914834 1103 84-85 0.4608092780597062 1103 86-87 0.3757640801058919 1103 88-89 0.42068529915690434 1103 90-91 0.30635764376595276 1103 92-93 0.16037700703182622 1103 94-95 0.20780007094916897 1103 96-97 0.16138089837790304 1103 98-99 0.3844291331988998 1103 100-101 0.5569046901231438 1104 1 0.09518837411936332 1104 2 0.07167573024462826 1104 3 0.03340259415189095 1104 4 0.13991971693086924 1104 5 0.134825802778046 1104 6 0.10312195809742519 1104 7 0.17435264077553114 1104 8 0.07982982622326773 1104 9 0.1907397286481043 1104 10-11 0.21453742831494083 1104 12-13 0.2108228942554362 1104 14-15 0.26979525737803556 1104 16-17 0.24969203589794375 1104 18-19 0.20175228243520138 1104 20-21 0.3788455738972303 1104 22-23 0.2527502594579616 1104 24-25 0.20556720381082272 1104 26-27 0.29870068200084177 1104 28-29 0.30701307177498904 1104 30-31 0.3356307873478457 1104 32-33 0.379620565600824 1104 34-35 0.48870070984707326 1104 36-37 0.5953938943053529 1104 38-39 0.7423954493679403 1104 40-41 0.7681712314971492 1104 42-43 0.6743463295025194 1104 44-45 0.6694136260577963 1104 46-47 0.5990927896708698 1104 48-49 0.7502798963971529 1104 50-51 0.7146128475872331 1104 52-53 0.7830146487015455 1104 54-55 0.7484115944818086 1104 56-57 0.5554533755577893 1104 58-59 0.6123970231811882 1104 60-61 0.5253266004485937 1104 62-63 0.6056935442720643 1104 64-65 0.5978724063553926 1104 66-67 0.49774168088324444 1104 68-69 0.5663035407790211 1104 70-71 0.6088103377678422 1104 72-73 0.43233242549185746 1104 74-75 0.37018545879051246 1104 76-77 0.5301624897009241 1104 78-79 0.5343029652935058 1104 80-81 0.48345702396859025 1104 82-83 0.5134117048630245 1104 84-85 0.7113999442786181 1104 86-87 0.550791596726409 1104 88-89 0.7844974475805415 1104 90-91 0.6910311849070361 1104 92-93 0.6888075617740661 1104 94-95 0.6936618759677735 1104 96-97 0.4387493294943319 1104 98-99 0.5326364597306572 1104 100-101 0.7556646197092753 1105 1 0.019945910742833917 1105 2 0.08006357356412863 1105 3 0.05159055844035976 1105 4 0.11018832408298351 1105 5 0.2024037845579727 1105 6 0.16516525938254745 1105 7 0.13349817484884596 1105 8 0.09662361150101617 1105 9 0.24015018008653755 1105 10-11 0.26729787940307403 1105 12-13 0.2832860489299094 1105 14-15 0.34718094987866266 1105 16-17 0.373533431104498 1105 18-19 0.29182132582288745 1105 20-21 0.32329326182532725 1105 22-23 0.24569762861648314 1105 24-25 0.2309251481791108 1105 26-27 0.2605035049214308 1105 28-29 0.2859617276883455 1105 30-31 0.34790370781424684 1105 32-33 0.3840769175224281 1105 34-35 0.37281570976534084 1105 36-37 0.6259076154088348 1105 38-39 0.49314449948131767 1105 40-41 0.5095778810537936 1105 42-43 0.4711628373293095 1105 44-45 0.6588326364008736 1105 46-47 0.7460785489613144 1105 48-49 0.7346126054247506 1105 50-51 0.9333343130398504 1105 52-53 0.8503453797230875 1105 54-55 0.819910850686135 1105 56-57 0.7975895170214216 1105 58-59 0.7473892232517159 1105 60-61 0.7690638876144433 1105 62-63 0.6825920330941173 1105 64-65 0.5688305132611333 1105 66-67 0.548054437037365 1105 68-69 0.5566301102812936 1105 70-71 0.6245050686118958 1105 72-73 0.626080396692835 1105 74-75 0.476307702834891 1105 76-77 0.43275211299235394 1105 78-79 0.5927303337047256 1105 80-81 0.6504166745130675 1105 82-83 0.46748874434607757 1105 84-85 0.5392637911384455 1105 86-87 0.6184956851505206 1105 88-89 0.6493245530360525 1105 90-91 0.6461159098894633 1105 92-93 0.5892714707530935 1105 94-95 0.8794149194582168 1105 96-97 0.8770037800079855 1105 98-99 1.1078036094169086 1105 100-101 1.5676080865677484 1106 1 -0.12708383776408994 1106 2 -0.19140111068145416 1106 3 -0.10919689095459617 1106 4 -0.06115290214109592 1106 5 -0.12005216564212162 1106 6 -0.11446826756044715 1106 7 -0.16980515840690913 1106 8 -0.16789905257680005 1106 9 -0.15085212394601655 1106 10-11 -0.17917603094689127 1106 12-13 -0.1262690367315642 1106 14-15 -0.00670664790973774 1106 16-17 0.011754881991144828 1106 18-19 -0.08998368807998958 1106 20-21 -0.017473596456170526 1106 22-23 0.002612219825252282 1106 24-25 -0.02238849171578039 1106 26-27 -0.2288399289182621 1106 28-29 -0.25251694588044415 1106 30-31 -0.3768624810898231 1106 32-33 -0.15972186585085524 1106 34-35 -0.11317197103029031 1106 36-37 -0.07048373100396077 1106 38-39 -0.001954582784499337 1106 40-41 0.25296528545417374 1106 42-43 0.12016719382376095 1106 44-45 0.14553873427404795 1106 46-47 0.20899875230132636 1106 48-49 0.11025857126212202 1106 50-51 0.17278967919705224 1106 52-53 0.08242346415108415 1106 54-55 0.05216098949329506 1106 56-57 0.2558889104812465 1106 58-59 0.3778219580242137 1106 60-61 0.31130836331127654 1106 62-63 0.14287988755106795 1106 64-65 0.029160480867254535 1106 66-67 0.012042984477268703 1106 68-69 -0.04756779492200636 1106 70-71 0.13778871951161875 1106 72-73 0.24053146841707118 1106 74-75 -0.007692071083990726 1106 76-77 0.11529488277417599 1106 78-79 0.03071705919681378 1106 80-81 -0.04423639625586162 1106 82-83 0.03518646281081317 1106 84-85 0.24082397249096132 1106 86-87 0.26538357883920227 1106 88-89 0.3071308347663724 1106 90-91 0.24579911926606712 1106 92-93 0.24063831950372006 1106 94-95 0.33072131367497093 1106 96-97 0.4070019181634912 1106 98-99 0.45111581968121683 1106 100-101 0.8131959030120477 1107 1 -0.001541744858062799 1107 2 0.026630593885677456 1107 3 0.03395659039641785 1107 4 0.029386027124751024 1107 5 0.20560294975241078 1107 6 0.007423957580790841 1107 7 0.05689645947482802 1107 8 0.052725132806472175 1107 9 -0.01791326988190889 1107 10-11 0.062354978498945 1107 12-13 0.11803228803933052 1107 14-15 0.187243735437697 1107 16-17 0.0941957444582684 1107 18-19 0.32027200331144456 1107 20-21 0.14189127322989492 1107 22-23 0.22082959241520683 1107 24-25 0.009066502410455257 1107 26-27 -0.09279323128035344 1107 28-29 -0.059099241479330544 1107 30-31 -0.05553009107648421 1107 32-33 0.016550887758462807 1107 34-35 -0.06825693854457171 1107 36-37 -0.010578376343673312 1107 38-39 0.02222060317212282 1107 40-41 -0.09540784487845855 1107 42-43 -0.03426091293410849 1107 44-45 0.13876645534818266 1107 46-47 0.23177778618993017 1107 48-49 0.37044365108425836 1107 50-51 0.4539380668400028 1107 52-53 0.25902962310710365 1107 54-55 0.2730780383529847 1107 56-57 0.44033669172776513 1107 58-59 0.5288181611062726 1107 60-61 0.5346953053658083 1107 62-63 0.6384469432547775 1107 64-65 0.7158449364820143 1107 66-67 0.5059292704406602 1107 68-69 0.5498776824468372 1107 70-71 0.5952493031936115 1107 72-73 0.49077265818765525 1107 74-75 0.6686382657241232 1107 76-77 0.470925145315082 1107 78-79 0.4101697247079663 1107 80-81 0.2957262887318812 1107 82-83 0.26080925465318217 1107 84-85 0.5368416064179407 1107 86-87 0.35015662957233573 1107 88-89 0.46130304673631173 1107 90-91 0.6521971486288223 1107 92-93 0.7463446908166738 1107 94-95 0.6402136909787828 1107 96-97 0.8220107659113189 1107 98-99 0.9032162183933288 1107 100-101 1.3580653087149521 1108 1 0.32847364525614964 1108 2 0.4265537047276595 1108 3 0.21464488346938992 1108 4 0.23336430409521824 1108 5 0.4411321891270248 1108 6 0.3778875279644609 1108 7 0.43106569423118657 1108 8 0.41984998188386413 1108 9 0.39085700717699723 1108 10-11 0.501272008219189 1108 12-13 0.44752244291079535 1108 14-15 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-1.8727193651902176 2316 74-75 -1.9366155044501987 2316 76-77 -1.9436590248741403 2316 78-79 -1.7185062118834047 2316 80-81 -1.5759711343283662 2316 82-83 -1.6890752924869616 2316 84-85 -1.7933244435247317 2316 86-87 -1.8460278396238294 2316 88-89 -1.9263379108307745 2316 90-91 -1.9311857531750611 2316 92-93 -1.7519173978160048 2316 94-95 -1.5825190835343328 2316 96-97 -1.9323693932220962 2316 98-99 -2.0873892706719026 2316 100-101 -2.0549832586321024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9442.0 3 1808.0 4 1647.0 5 1711.0 6 2141.0 7 2617.0 8 3189.0 9 3769.0 10 4414.0 11 6121.0 12 8224.0 13 10866.0 14 11460.0 15 13651.0 16 17931.0 17 22580.0 18 19977.0 19 13264.0 20 12892.0 21 13495.0 22 14690.0 23 16291.0 24 18467.0 25 21029.0 26 23929.0 27 28036.0 28 32977.0 29 38683.0 30 46709.0 31 56770.0 32 70656.0 33 90974.0 34 125615.0 35 203921.0 36 239772.0 37 316517.0 38 256745.0 39 39856.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.90895398741621 18.92987159867407 14.14152860397801 19.019645809931713 2 14.365498494704957 22.81515174659832 40.75863133816032 22.060718420536414 3 20.6523746498815 30.221987222048778 29.61562814666942 19.510009981400298 4 11.634306412121799 17.29865137521924 38.754445230800336 32.31259698185862 5 11.864998586839018 40.98843187519314 32.76740909855725 14.379160439410585 6 26.56750856333792 37.34676132141428 19.344459443416014 16.741270671831785 7 25.839634567808904 36.86253786651959 20.846340426678488 16.451487138993016 8 25.189639945315946 32.745762040775475 20.658793277336805 21.405804736571778 9 28.07479315687115 18.48023865247058 21.318741690564877 32.126226500093395 10-11 23.726658124492566 27.669635539402016 26.533372471222382 22.070333864883036 12-13 28.461228501660717 25.794846225648595 26.685509379944083 19.05841589274661 14-15 25.10803527467117 25.908518411057184 28.614153610114652 20.369292704156994 16-17 19.484452938513176 29.928994206539 29.783181193779747 20.803371661168075 18-19 19.17818199213229 29.427951126615476 32.84946193695 18.544404944302233 20-21 21.062788549561887 27.61674379643348 31.415326411579166 19.90514124242547 22-23 20.187936196497148 27.53906470177584 30.740045515797586 21.53295358592942 24-25 20.48096315797577 27.845244143567548 31.868372955389724 19.805419743066967 26-27 21.37489290342965 26.391016906189595 32.82966409176191 19.404426098618842 28-29 19.610085874108172 26.882420433690385 34.003202738286205 19.504290953915238 30-31 19.577102069761544 28.976414038133875 32.56500567491303 18.881478217191546 32-33 19.27680647924411 28.864233377659392 31.76746654179524 20.09149360130126 34-35 20.00045143955541 27.483062070461457 31.9975126781569 20.51897381182623 36-37 21.186683379999955 27.262743733050765 32.58860416496853 18.961968721980753 38-39 21.806640184049417 26.531964709911822 32.76213440937779 18.899260696660967 40-41 19.69058698804094 27.59839555060885 33.746500519073614 18.964516942276592 42-43 19.168602351013334 29.132753761403833 31.977239270407832 19.721404617175 44-45 19.494560186523625 29.32322960324632 30.7329774890788 20.449232721151255 46-47 19.711284557107856 28.615634040083243 29.913606197797087 21.75947520501181 48-49 20.493068547257725 28.491813816197322 30.198369235184135 20.816748401360815 50-51 21.25388187532891 28.947905418969906 30.47292442731926 19.325288278381926 52-53 19.7919690159354 29.2826287987231 31.05145119387457 19.873950991466934 54-55 18.70522377476607 28.99581471285802 31.523206052763975 20.77575545961194 56-57 20.09049337749256 29.96828464530836 29.38391822283891 20.557303754360174 58-59 19.909681730904737 28.672926428621757 30.20457167770575 21.212820162767756 60-61 19.989428663565153 28.13552112121102 31.037630876006645 20.83741933921718 62-63 21.717037804303537 27.81686156245379 30.4645642646728 20.001536368569862 64-65 20.76746550856075 28.373209326101783 31.244016397686874 19.615308767650593 66-67 19.616508611547985 28.62903825549015 31.167138123555677 20.587315009406186 68-69 19.31950179580812 30.044218280139457 30.581790045667155 20.05448987838527 70-71 19.70325409054425 29.634733583697432 29.341821913891568 21.32019041186675 72-73 19.761569201201187 28.92771967359813 28.93909476831025 22.371616356890428 74-75 19.946503503822203 29.47127042060188 29.52775791733822 21.0544681582377 76-77 20.84601421139533 28.0028484473979 30.428462223925855 20.72267511728091 78-79 19.865173500951993 27.941466347609772 31.409916164974295 20.783443986463944 80-81 19.512444262726135 30.02289375398369 30.099518308808243 20.36514367448193 82-83 19.566542103786368 29.475748237776227 29.361281825558915 21.596427832878486 84-85 19.70076007799315 28.371178441684535 29.43938499851718 22.488676481805133 86-87 20.545413894146495 28.305560863644047 30.124754458840226 21.02427078336923 88-89 21.313857598282123 27.062566312878655 30.36605948187767 21.257516606961556 90-91 19.966640372640697 27.71228753619101 31.292333014573355 21.02873907659494 92-93 19.5781533903041 29.213432703041324 30.267732488402537 20.940681418252037 94-95 19.419604429747785 29.26721306282546 29.52883316278538 21.784349344641374 96-97 19.5642156032354 28.09887989555057 28.962625999699764 23.37427850151427 98-99 20.408109301435847 27.89301446861864 29.08324833695859 22.615627892986925 100-101 21.459788368384515 27.531936536399982 29.602045876923 21.406229218292502 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16336.0 1 10175.5 2 7189.5 3 9009.5 4 5944.0 5 4965.5 6 8093.5 7 15127.0 8 21288.5 9 20716.5 10 14418.5 11 8993.5 12 7687.0 13 7390.0 14 6938.5 15 6353.5 16 5848.0 17 5558.5 18 5392.0 19 5378.0 20 5568.0 21 5933.0 22 6450.0 23 7192.0 24 7854.0 25 8741.5 26 10407.5 27 12413.0 28 13761.5 29 15284.5 30 17830.0 31 20462.5 32 24328.5 33 29162.5 34 33720.0 35 37018.0 36 42137.0 37 47292.5 38 48177.0 39 48226.5 40 47496.5 41 45967.5 42 44875.0 43 45638.0 44 49811.0 45 53311.0 46 56156.5 47 67410.0 48 126669.5 49 127796.5 50 76722.5 51 64138.0 52 48879.5 53 43250.5 54 40441.5 55 37412.5 56 34510.5 57 30799.0 58 27242.5 59 24346.0 60 21554.0 61 18596.5 62 15351.5 63 11825.5 64 8515.0 65 6180.5 66 4687.5 67 3623.0 68 2798.5 69 2135.0 70 1662.5 71 1318.0 72 1058.5 73 955.0 74 807.0 75 620.0 76 447.5 77 301.5 78 224.5 79 154.0 80 126.5 81 117.0 82 84.0 83 57.5 84 43.0 85 36.0 86 23.0 87 17.5 88 13.0 89 6.0 90 6.5 91 6.0 92 5.5 93 4.5 94 2.5 95 1.5 96 2.5 97 2.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27138955070683624 2 0.1608478194203444 3 0.1896490149168249 4 0.1598054904404718 5 0.2316713306316898 6 0.1896490149168249 7 0.23490803641129424 8 0.24110715087053672 9 0.14378653980242928 10-11 0.19924941341565172 12-13 0.20555824671488077 14-15 0.1681441222794528 16-17 0.2798927608057971 18-19 0.22720028790223615 20-21 0.19225483736650648 22-23 0.23872076610082835 24-25 0.22805060891213222 26-27 0.3368094090488419 28-29 0.30973628528215025 30-31 0.28483010860519375 32-33 0.1848488156674115 34-35 0.35271864084689775 36-37 0.39087336745223533 38-39 0.3734280718943671 40-41 0.33700141701881847 42-43 0.3692313262648799 44-45 0.3773230907138911 46-47 0.38237015735327434 48-49 0.4011595087009783 50-51 0.3945215188817895 52-53 0.39487810511174587 54-55 0.3881578261625671 56-57 0.36679008207517827 58-59 0.3436942662580006 60-61 0.3106688954220363 62-63 0.3769116450639414 64-65 0.4094158514099694 66-67 0.413969183269413 68-69 0.41920825787877275 70-71 0.41791906150893027 72-73 0.4098821564799124 74-75 0.4056854108504253 76-77 0.3903247732523023 78-79 0.36508944005538607 80-81 0.3754304407241224 82-83 0.385359995742909 84-85 0.3862377464628018 86-87 0.38149240663338163 88-89 0.39013276528232577 90-91 0.40996444560990236 92-93 0.4095529999599526 94-95 0.4176721941189605 96-97 0.417397897018994 98-99 0.4151486607992689 100-101 0.3890355768824598 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1822841.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.63045125769184 #Duplication Level Percentage of deduplicated Percentage of total 1 68.72746487799559 23.80063122522154 2 14.028280823455361 9.71611390571768 3 6.1403893580079 6.379333630972268 4 3.2825331629980803 4.547024188118482 5 1.9404752526570188 3.3599766826948074 6 1.2716381926247835 2.6422442668267148 7 0.8609225378570956 2.086989518873595 8 0.6363758611789906 1.7630386593704563 9 0.485123254378467 1.5120033493253706 >10 2.315743908190161 14.2967262989631 >50 0.1874377916728131 4.500188069143784 >100 0.10540822627839268 6.795742372143873 >500 0.007985417853217642 1.8228319922324276 >1k 0.007666001139088936 5.695368263612066 >5k 0.0014373752135791757 3.4830039659820717 >10k+ 0.00111795849945047 7.598783610801791 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26670 1.4631007312212092 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 24634 1.3514069521148582 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 21014 1.1528158517391258 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19538 1.0718433478290208 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15448 0.8474683200564394 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15279 0.8381970780775723 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 14808 0.8123582912607298 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9158 0.5024025682986064 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 8421 0.4619711757635471 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8108 0.4448001773056454 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7336 0.40244870507082076 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6572 0.3605361081959425 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5965 0.3272364402600117 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5931 0.3253712199802396 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5771 0.31659371278131226 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5713 0.3134118664217011 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4912 0.26946947100707086 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4785 0.26250232466792217 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4672 0.25630321020867974 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4593 0.25196931602920936 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4194 0.23008040745188416 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4007 0.21982169591313777 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3816 0.20934354669441824 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3459 0.1897587337568115 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3360 0.18432765117747515 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3088 0.1694058889392986 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2897 0.15892773972057903 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2896 0.15887288030058572 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2695 0.1478461368819332 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2626 0.14406083690239577 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2582 0.14164702242269073 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2556 0.14022067750286504 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2528 0.13868461374305274 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1945 0.10670157188696107 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1935 0.10615297768702811 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1910 0.10478149218719571 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.485941999329618E-5 0.0 2 0.0 0.0 0.0 5.485941999329618E-5 0.0 3 0.0 0.0 0.0 5.485941999329618E-5 0.0 4 0.0 0.0 0.0 5.485941999329618E-5 0.0 5 0.0 0.0 0.0 5.485941999329618E-5 0.0 6 0.0 0.0 0.0 5.485941999329618E-5 0.0 7 0.0 0.0 0.0 5.485941999329618E-5 0.0 8 0.0 0.0 0.0 5.485941999329618E-5 0.0 9 0.0 0.0 0.0 5.485941999329618E-5 0.0 10-11 0.0 0.0 0.0 5.485941999329618E-5 0.0 12-13 0.0 0.0 0.0 5.485941999329618E-5 0.0 14-15 0.0 0.0 0.0 8.228912998994428E-5 0.0 16-17 0.0 0.0 0.0 3.01726809963129E-4 0.0 18-19 0.0 0.0 0.0 3.5658622995642515E-4 0.0 20-21 0.0 0.0 0.0 7.406021699094984E-4 0.0 22-23 0.0 0.0 0.0 0.002166947089735199 0.0 24-25 0.0 0.0 0.0 0.004443613019456991 0.0 26-27 0.0 0.0 0.0 0.007625459379068169 0.0 28-29 0.0 0.0 0.0 0.013303409348374323 0.0 30-31 0.0 0.0 0.0 0.028636617236500607 0.0 32-33 0.0 0.0 0.0 0.052637613483567686 0.0 34-35 0.0 0.0 0.0 0.09485193716840909 0.0 36-37 0.0 0.0 0.0 0.1545115564111187 0.0 38-39 0.0 0.0 0.0 0.232357073381606 0.0 40-41 0.0 0.0 0.0 0.31944640262096363 0.0 42-43 0.0 0.0 0.0 0.40645344273033135 0.0 44-45 0.0 0.0 0.0 0.5032528893085025 0.0 46-47 0.0 0.0 0.0 0.628853531383154 0.0 48-49 0.0 0.0 0.0 0.7798266552047053 0.0 50-51 0.0 0.0 0.0 0.9320066862661087 0.0 52-53 0.0 0.0 0.0 1.0850918977574018 0.0 54-55 0.0 0.0 0.0 1.239795462138497 0.0 56-57 0.0 0.0 0.0 1.423190503176086 0.0 58-59 0.0 0.0 0.0 1.651103963538235 0.0 60-61 0.0 0.0 0.0 1.9525016169814045 0.0 62-63 0.0 0.0 0.0 2.227594178537788 0.0 64-65 0.0 0.0 0.0 2.464532013488834 0.0 66-67 0.0 0.0 0.0 2.7377044953454526 0.0 68-69 0.0 0.0 0.0 3.047029334977653 0.0 70-71 0.0 0.0 0.0 3.4118444779330726 0.0 72-73 0.0 0.0 0.0 3.7933917439864473 0.0 74-75 0.0 0.0 0.0 4.165283752121002 0.0 76-77 0.0 0.0 0.0 4.537532346485513 0.0 78-79 0.0 0.0 0.0 4.960580763763817 0.0 80-81 0.0 0.0 0.0 5.42954651557651 0.0 82-83 0.0 0.0 0.0 5.909209854287894 0.0 84-85 0.0 0.0 0.0 6.464661481720018 0.0 86-87 0.0 0.0 0.0 7.059639321257312 0.0 88-89 0.0 0.0 0.0 7.604530510340727 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTACG 80 2.2539825E-7 41.58496 1 TACCTGG 1360 0.0 34.185207 2 TATAACG 115 1.0484109E-7 33.002224 2 ATCACGC 105 1.9211184E-6 31.627129 3 TATCACG 110 2.7613642E-6 30.189533 2 GTATAGG 725 0.0 24.910006 1 CATGGGG 4355 0.0 23.529715 4 GAGTACT 14535 0.0 23.431005 12-13 AGTACTT 15050 0.0 22.10882 12-13 ATGGGAT 4365 0.0 22.06291 5 ACCTGGG 1945 0.0 21.95199 3 TAGCGCT 130 2.96738E-4 21.895706 4 GTAGCGC 130 2.96738E-4 21.895706 3 GTACTTT 15930 0.0 21.274555 14-15 GTACATG 24615 0.0 21.006674 1 GTACGGG 115 0.0037124592 20.663334 1 TACATGG 24740 0.0 20.652233 2 ATGGGAG 3920 0.0 20.45275 5 CCCTATA 325 2.1827873E-11 20.435991 2 TAACGCA 260 1.5130354E-8 20.071064 4 >>END_MODULE