##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938694_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1822841 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.933648628706507 31.0 31.0 34.0 30.0 34.0 2 32.10092377777327 33.0 31.0 34.0 30.0 34.0 3 32.158639179171416 34.0 31.0 34.0 30.0 34.0 4 35.811510164627634 37.0 35.0 37.0 35.0 37.0 5 35.73478707139021 37.0 35.0 37.0 33.0 37.0 6 35.80518322771981 37.0 35.0 37.0 33.0 37.0 7 35.726040834060676 37.0 35.0 37.0 33.0 37.0 8 35.735860670239475 37.0 35.0 37.0 33.0 37.0 9 37.32400631761081 39.0 37.0 39.0 34.0 39.0 10-11 37.23573888232709 39.0 37.0 39.0 33.0 39.0 12-13 37.14328539900079 39.0 37.0 39.0 33.0 39.0 14-15 38.17244839237213 40.0 37.0 41.0 33.0 41.0 16-17 38.115765719555355 40.0 37.0 41.0 33.0 41.0 18-19 38.373059965186215 40.0 38.0 41.0 33.5 41.0 20-21 38.39719372122966 40.0 38.0 41.0 34.0 41.0 22-23 38.29728155116108 40.0 37.5 41.0 33.5 41.0 24-25 38.186131154609754 40.0 37.0 41.0 33.0 41.0 26-27 37.89133007212368 40.0 37.0 41.0 33.0 41.0 28-29 37.6702087565509 40.0 36.5 41.0 32.5 41.0 30-31 37.382915734285106 39.0 36.0 41.0 32.0 41.0 32-33 37.10458125530422 39.0 35.0 41.0 32.0 41.0 34-35 37.008066254818715 39.0 35.0 41.0 31.0 41.0 36-37 36.80371793261179 39.0 35.0 41.0 31.0 41.0 38-39 36.606300000932606 39.0 35.0 41.0 30.5 41.0 40-41 36.36964606348003 39.0 35.0 41.0 30.0 41.0 42-43 36.16570342668395 39.0 35.0 41.0 30.0 41.0 44-45 35.86060276239124 39.0 35.0 41.0 29.5 41.0 46-47 35.407793384063666 38.0 35.0 40.0 26.5 41.0 48-49 35.010668237108995 38.0 34.5 40.0 25.0 41.0 50-51 34.78155692131129 38.0 34.0 40.0 24.0 41.0 52-53 34.495467240423054 38.0 34.0 40.0 24.0 41.0 54-55 34.241508721824886 38.0 34.0 40.0 23.0 41.0 56-57 34.03396922715695 38.0 33.0 40.0 23.0 41.0 58-59 33.7651166503277 37.0 33.0 40.0 22.5 41.0 60-61 33.361226788293656 36.5 33.0 40.0 22.0 41.0 62-63 32.93348898779433 36.0 32.5 39.0 20.5 41.0 64-65 32.45461973918734 35.5 32.0 39.0 19.0 40.0 66-67 32.038737607942764 35.0 31.0 38.5 18.0 40.0 68-69 31.75393136318527 35.0 31.0 38.0 15.5 40.0 70-71 31.307808250966488 35.0 31.0 37.0 11.5 39.5 72-73 30.77500972383219 35.0 30.5 37.0 7.5 39.0 74-75 30.266614861087717 34.0 30.0 36.0 4.5 39.0 76-77 28.31432445287329 32.0 27.0 34.5 2.0 36.5 78-79 29.25170023057414 34.0 29.0 35.0 2.0 37.0 80-81 29.35179727688811 34.0 29.0 35.0 2.0 37.0 82-83 29.04615679590266 34.0 29.0 35.0 2.0 36.0 84-85 28.65249053537857 34.0 29.0 35.0 2.0 36.0 86-87 28.017466690731666 33.5 27.5 35.0 2.0 35.5 88-89 27.31920529547009 33.0 26.0 35.0 2.0 35.0 90-91 26.712142748599575 33.0 25.0 35.0 2.0 35.0 92-93 26.41260866965358 33.0 25.0 35.0 2.0 35.0 94-95 26.067968901291994 33.0 24.5 35.0 2.0 35.0 96-97 25.34168558859495 32.0 21.5 34.0 2.0 35.0 98-99 24.430726541700565 32.0 9.5 34.0 2.0 35.0 100-101 20.78904715222008 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07213530438666638 1101 2 0.10333627386418698 1101 3 -0.013910198107282667 1101 4 0.09634288249412037 1101 5 -0.06179424777000975 1101 6 0.1469582076030207 1101 7 0.1938035153989901 1101 8 0.23211389062237942 1101 9 0.19328772019130014 1101 10-11 0.27549798611910603 1101 12-13 0.18267684050582034 1101 14-15 0.28093312327112585 1101 16-17 0.36360929436537504 1101 18-19 0.3163068748856901 1101 20-21 0.3639908825454157 1101 22-23 0.23750880498841553 1101 24-25 0.31410473161859187 1101 26-27 0.1848664524124075 1101 28-29 0.21764429460013446 1101 30-31 0.3262953312311865 1101 32-33 0.4262685852297423 1101 34-35 0.4881555447996604 1101 36-37 0.3301883315837202 1101 38-39 0.4840023341321924 1101 40-41 0.46825526911004545 1101 42-43 0.5957921516232858 1101 44-45 0.6515150140973063 1101 46-47 0.682572292766018 1101 48-49 0.5784915540568534 1101 50-51 0.41358077508962765 1101 52-53 0.32373169059422935 1101 54-55 0.48561105930075144 1101 56-57 0.503298312236403 1101 58-59 0.4311266682621522 1101 60-61 0.5067902092893704 1101 62-63 0.36330466652273685 1101 64-65 0.3747246203491912 1101 66-67 0.3140607087251581 1101 68-69 0.3446587256833702 1101 70-71 0.42886604055469846 1101 72-73 0.2781045735471963 1101 74-75 0.10779897584383136 1101 76-77 0.09453533755523935 1101 78-79 0.34423900951330566 1101 80-81 0.528183592458138 1101 82-83 0.5490786263041265 1101 84-85 0.6903539416852595 1101 86-87 0.6778796548992894 1101 88-89 0.7224947095992995 1101 90-91 0.6966765162183997 1101 92-93 0.5748203950414883 1101 94-95 0.7750427312932899 1101 96-97 0.7098547543606806 1101 98-99 0.7112349404587732 1101 100-101 1.2080944359942158 1102 1 0.277058201828563 1102 2 0.1406655844285467 1102 3 0.19641790975767748 1102 4 0.10651480283910786 1102 5 -0.181090250867868 1102 6 -0.027891963676047737 1102 7 0.08441459830014963 1102 8 0.12458097208314456 1102 9 0.13051889714411402 1102 10-11 0.1984083768438012 1102 12-13 0.24653336503444478 1102 14-15 0.27198471294006765 1102 16-17 0.33124632147497834 1102 18-19 0.3216729305218706 1102 20-21 0.3219628434017281 1102 22-23 0.3359923431752705 1102 24-25 0.23321217512855696 1102 26-27 0.23564974854869547 1102 28-29 0.24472322109303235 1102 30-31 0.3651498286517523 1102 32-33 0.3818184987963136 1102 34-35 0.593033028854876 1102 36-37 0.4362362971225764 1102 38-39 0.5286013320779546 1102 40-41 0.5076533089659208 1102 42-43 0.39146305039830054 1102 44-45 0.3576891690398867 1102 46-47 0.4229729726411051 1102 48-49 0.6723555981543967 1102 50-51 0.6612300234256168 1102 52-53 0.45170217466763063 1102 54-55 0.5765267787761275 1102 56-57 0.6202584394062214 1102 58-59 0.6717499446422295 1102 60-61 0.6714030994768407 1102 62-63 0.573596548502195 1102 64-65 0.41605989723981907 1102 66-67 0.5296463487224798 1102 68-69 0.6283450224637086 1102 70-71 0.5664433211591735 1102 72-73 0.2953624312500658 1102 74-75 0.37341240957373856 1102 76-77 0.4678248976866648 1102 78-79 0.33615262110460264 1102 80-81 0.601667415827503 1102 82-83 0.52575593875655 1102 84-85 0.7347593238344352 1102 86-87 0.6249880122264919 1102 88-89 0.6088619685514871 1102 90-91 0.5864265021134258 1102 92-93 0.7426149970454112 1102 94-95 0.6737406650612705 1102 96-97 0.7399872917727741 1102 98-99 1.0346164835088416 1102 100-101 1.7203990590029896 1103 1 0.2596288125772368 1103 2 0.19222525074892616 1103 3 0.16906272621184826 1103 4 -0.019808191087435034 1103 5 -0.06270378644261854 1103 6 0.01569283096232965 1103 7 0.07946664554616945 1103 8 0.041574981343835304 1103 9 0.05330770811082175 1103 10-11 0.1522970740217886 1103 12-13 0.18566787031011955 1103 14-15 0.1942273742617715 1103 16-17 0.12488402530367182 1103 18-19 -0.06289597203736719 1103 20-21 0.1320106986699514 1103 22-23 0.22494584014820163 1103 24-25 0.20471382097421298 1103 26-27 0.13851013561150438 1103 28-29 0.09122633873499808 1103 30-31 0.23901160176929892 1103 32-33 0.21454726349276854 1103 34-35 0.1297956324882037 1103 36-37 0.17490942822052347 1103 38-39 0.02150999104055984 1103 40-41 0.29998315195317815 1103 42-43 0.403547781183363 1103 44-45 0.5708153881778273 1103 46-47 0.5321976182702883 1103 48-49 0.5194869841276173 1103 50-51 0.6321397807256517 1103 52-53 0.5197634702287814 1103 54-55 0.4760100348665688 1103 56-57 0.40052226983838324 1103 58-59 0.3835408294076714 1103 60-61 0.38021386088914966 1103 62-63 0.28407489286871623 1103 64-65 0.3651147624851703 1103 66-67 0.4430813280826058 1103 68-69 0.4179507591785132 1103 70-71 0.4322568592095628 1103 72-73 0.6254332072927866 1103 74-75 0.583249503610805 1103 76-77 0.6370555089968839 1103 78-79 0.4853888418456691 1103 80-81 0.5502488909991321 1103 82-83 0.514560323894397 1103 84-85 0.4701708033625813 1103 86-87 0.3912918169482573 1103 88-89 0.4564057593976081 1103 90-91 0.40630218287582665 1103 92-93 0.33555689596153826 1103 94-95 0.31417071337543945 1103 96-97 0.4954695443245676 1103 98-99 0.811927664118933 1103 100-101 1.3006273549889222 1104 1 0.16259254083133712 1104 2 0.08605322197628595 1104 3 0.12202812757208648 1104 4 -0.0644532356458356 1104 5 -0.0916843748262437 1104 6 0.10074518125816923 1104 7 0.09284073893178402 1104 8 0.030272850643171978 1104 9 0.06598985260266232 1104 10-11 0.17511749476017258 1104 12-13 0.14329212532972946 1104 14-15 0.14098958524762395 1104 16-17 0.24074408551159365 1104 18-19 0.2768085096164299 1104 20-21 0.22974220576811177 1104 22-23 0.08989136859279512 1104 24-25 0.07409815974474299 1104 26-27 0.16403919567242298 1104 28-29 0.03752949122547733 1104 30-31 0.06337384956348302 1104 32-33 0.1828380182667999 1104 34-35 0.10161006828693786 1104 36-37 0.18368008958743332 1104 38-39 0.1806751170553298 1104 40-41 0.294277113115605 1104 42-43 0.32055633912122516 1104 44-45 0.2613829237044456 1104 46-47 0.21154757470978325 1104 48-49 0.29910499607120045 1104 50-51 0.32514437459707324 1104 52-53 0.2419295570845037 1104 54-55 0.2863095268398439 1104 56-57 0.08781111488126925 1104 58-59 0.2530482609865885 1104 60-61 0.4517596137689921 1104 62-63 0.24124950061495554 1104 64-65 0.21962488896004118 1104 66-67 0.21196158387663644 1104 68-69 0.24156100551949677 1104 70-71 0.32710542517834185 1104 72-73 0.472961700887776 1104 74-75 0.44055043087012535 1104 76-77 0.3886863393236055 1104 78-79 0.291363952001781 1104 80-81 0.4256852430541258 1104 82-83 0.5051550772725726 1104 84-85 0.5429259729617648 1104 86-87 0.5487482833241977 1104 88-89 0.5628032093448461 1104 90-91 0.47989315861937243 1104 92-93 0.6218634484426921 1104 94-95 0.8693076272355711 1104 96-97 0.7679729712044541 1104 98-99 1.0900267347084736 1104 100-101 1.754305021531703 1105 1 0.14924800580288533 1105 2 0.17240065979286356 1105 3 0.1564529061264821 1105 4 0.03609346000979485 1105 5 0.028009512971543415 1105 6 -0.012847713800084648 1105 7 0.020722917961187193 1105 8 0.10105429148164546 1105 9 0.16805916922141506 1105 10-11 0.14673386667202237 1105 12-13 0.0670095740522001 1105 14-15 0.1016808014527868 1105 16-17 0.1660501276545574 1105 18-19 0.19721945380489814 1105 20-21 0.06840568551748305 1105 22-23 -0.01396883752150302 1105 24-25 -0.01951004325892569 1105 26-27 0.1332297661648667 1105 28-29 0.22272527179057278 1105 30-31 0.05239293837291825 1105 32-33 0.23731025028433805 1105 34-35 0.20832606444768942 1105 36-37 0.1695894235808808 1105 38-39 0.37529590970564897 1105 40-41 0.24386744167011898 1105 42-43 0.297795257499871 1105 44-45 0.3389814211671336 1105 46-47 0.16833707469693593 1105 48-49 0.29455026929058903 1105 50-51 0.18560241027590507 1105 52-53 0.24883798264305312 1105 54-55 0.34958149216518564 1105 56-57 0.4581259027535438 1105 58-59 0.36294990948066896 1105 60-61 0.3678014881807812 1105 62-63 0.47737747784103135 1105 64-65 0.5276167223871795 1105 66-67 0.4211027392470257 1105 68-69 0.36271066608244595 1105 70-71 0.3579636042451071 1105 72-73 0.3205364249127278 1105 74-75 0.34449424101890713 1105 76-77 0.42055074432209594 1105 78-79 0.22296131450467271 1105 80-81 0.41038780652196394 1105 82-83 0.5199480872279949 1105 84-85 0.5079958920900403 1105 86-87 0.743709447148408 1105 88-89 0.6123529035362303 1105 90-91 0.5378309880437904 1105 92-93 0.5506333365113711 1105 94-95 0.8253798956839162 1105 96-97 0.9969175403284076 1105 98-99 1.2524827433397725 1105 100-101 1.737592972041849 1106 1 0.016347218841115563 1106 2 -0.01964139572778123 1106 3 0.05674503839280476 1106 4 0.044237921410321235 1106 5 -0.23356412242389268 1106 6 -0.09198995761083495 1106 7 -0.007508961471081932 1106 8 -0.016104489497841712 1106 9 -0.06375376860295745 1106 10-11 -0.04292519413825602 1106 12-13 -0.03932834407318353 1106 14-15 -0.03002419372081988 1106 16-17 0.032939052007151304 1106 18-19 -0.22620166327963176 1106 20-21 -0.09013425160093647 1106 22-23 -0.023918900192519743 1106 24-25 -0.007173384095054303 1106 26-27 0.14043527057511795 1106 28-29 0.0753029289917535 1106 30-31 -0.04668378624365488 1106 32-33 -0.05325690301084762 1106 34-35 -0.030691546606348652 1106 36-37 -0.10782041826207944 1106 38-39 -0.013729846918032251 1106 40-41 0.06035191355460512 1106 42-43 0.143820164102884 1106 44-45 0.12767620168558835 1106 46-47 0.15976538648052951 1106 48-49 0.07323154035409374 1106 50-51 -0.03611528533690489 1106 52-53 0.058295376880863614 1106 54-55 -0.0014232943266918596 1106 56-57 -0.11281812997945195 1106 58-59 -0.007452692225356827 1106 60-61 -0.014323253183526674 1106 62-63 0.08514059359345083 1106 64-65 0.08028808134525889 1106 66-67 -0.031173642701503468 1106 68-69 -0.05075041416415971 1106 70-71 0.0250394888151817 1106 72-73 -0.03791019833306564 1106 74-75 0.03195510626680331 1106 76-77 0.14125938671607585 1106 78-79 -0.0369610421471549 1106 80-81 0.023202006632565997 1106 82-83 0.038585095155241333 1106 84-85 -0.050452725989135416 1106 86-87 5.182013638780347E-4 1106 88-89 0.03870393214990031 1106 90-91 -0.08570350207125799 1106 92-93 0.13514223623885258 1106 94-95 0.3858951057343347 1106 96-97 0.469778109232724 1106 98-99 0.7673301219624733 1106 100-101 1.0614159372286807 1107 1 -0.0982018428688356 1107 2 0.011844657593982788 1107 3 -0.06107733269367088 1107 4 -0.06119760272322594 1107 5 -0.14640982089023424 1107 6 -0.08693472996342422 1107 7 -0.03187531265328403 1107 8 0.022922626554155556 1107 9 -0.04932361138845209 1107 10-11 -0.10101372329019398 1107 12-13 -0.005730402885554042 1107 14-15 0.0868483981608037 1107 16-17 0.047956722765391646 1107 18-19 0.0604800712197644 1107 20-21 0.086504203421228 1107 22-23 0.07839833568200305 1107 24-25 0.028764441442426403 1107 26-27 0.022453480608938037 1107 28-29 0.09663564883726394 1107 30-31 0.05272556680704099 1107 32-33 -0.05518257313360664 1107 34-35 0.0015701620232135838 1107 36-37 -0.16872928728408 1107 38-39 -0.03395556756007778 1107 40-41 -0.1624348713898769 1107 42-43 -0.1616393278346493 1107 44-45 -0.1807696456449932 1107 46-47 -0.21826996273202326 1107 48-49 -0.21330286911165075 1107 50-51 -0.30400263335985755 1107 52-53 -0.371084808012057 1107 54-55 -0.2723035943860026 1107 56-57 -0.2555766373130055 1107 58-59 -0.08145933394571614 1107 60-61 -0.1270328134238028 1107 62-63 -0.09725219583565092 1107 64-65 -0.19427850544705905 1107 66-67 -0.23677754615523128 1107 68-69 -0.20953340493930384 1107 70-71 -0.20598636617850374 1107 72-73 -0.2598431302606521 1107 74-75 -0.2241395049902586 1107 76-77 -0.24522914859273115 1107 78-79 -0.08417266851107996 1107 80-81 -0.06679077554022328 1107 82-83 0.0317972866316083 1107 84-85 -0.11961110974555922 1107 86-87 0.08413381605834047 1107 88-89 -0.016981082530378444 1107 90-91 0.34773334358982666 1107 92-93 0.5127466047559324 1107 94-95 0.5225109012867648 1107 96-97 0.5942160889667356 1107 98-99 0.8493944260413429 1107 100-101 1.3571438685708195 1108 1 0.20628260058753867 1108 2 0.16074339850331398 1108 3 0.1530574370503217 1108 4 0.03135342232225469 1108 5 0.08356706323191077 1108 6 0.11679482409174113 1108 7 0.1660712800884241 1108 8 0.16596563166740452 1108 9 0.2500549498016795 1108 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-1.2776431422986292 2316 54-55 -1.2338170909317299 2316 56-57 -1.385051832239462 2316 58-59 -1.1804581661160967 2316 60-61 -1.1027704215278433 2316 62-63 -1.2857264623508584 2316 64-65 -1.4101913145960303 2316 66-67 -1.3690768138010299 2316 68-69 -1.4866497968512213 2316 70-71 -1.2076971434635517 2316 72-73 -1.4484113019821514 2316 74-75 -1.4696364444253867 2316 76-77 -1.443095930889605 2316 78-79 -1.3013410940160526 2316 80-81 -1.3257689645264499 2316 82-83 -1.4016618300210055 2316 84-85 -1.711644197364702 2316 86-87 -2.007458076703511 2316 88-89 -2.196966371601466 2316 90-91 -2.49384258794716 2316 92-93 -2.387068245035639 2316 94-95 -2.3772286647275287 2316 96-97 -2.491576691771261 2316 98-99 -2.709605177588241 2316 100-101 -2.5449038235690757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 14.0 4 53.0 5 156.0 6 372.0 7 744.0 8 1237.0 9 2060.0 10 3331.0 11 4777.0 12 6523.0 13 8899.0 14 10460.0 15 13441.0 16 17815.0 17 24001.0 18 22061.0 19 14860.0 20 13374.0 21 14198.0 22 15240.0 23 16461.0 24 19104.0 25 22285.0 26 26375.0 27 31561.0 28 37786.0 29 45021.0 30 53527.0 31 64204.0 32 78255.0 33 98216.0 34 134471.0 35 202741.0 36 245679.0 37 329908.0 38 226918.0 39 16712.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.84898802811054 20.032034275465076 15.013551059368982 19.1054266370554 2 14.76179216947611 23.768118009195536 39.40925182174419 22.060837999584166 3 20.678874350533043 29.88565651090797 30.105039331461164 19.330429807097822 4 12.054424933386949 18.22792004349255 37.95037526586246 31.767279757258038 5 11.694749232428162 41.217633711151535 32.98092251297121 14.106694543449095 6 26.74606535936238 37.95046478353991 18.571646448509533 16.73182340858818 7 25.38323419321817 36.787684718524545 20.840215904733324 16.98886518352396 8 25.212950553558976 33.649561316648025 20.31038362643807 20.827104503354928 9 27.321856376941266 18.640792038362093 21.09597052074207 32.94138106395456 10-11 23.775469171474636 27.992156200129358 26.297438997696453 21.934935630699552 12-13 28.253753344367393 26.005257726812157 26.686639152838893 19.054349775981557 14-15 24.714168706979926 26.30234891578585 28.48421776775923 20.49926460947499 16-17 19.304002154934764 30.14758121190941 29.79027240065109 20.758144232504733 18-19 19.055693831771396 29.455421509610545 32.88997778742085 18.59890687119721 20-21 20.767664321792193 27.728776124741543 31.597462422668794 19.906097130797477 22-23 19.993570475976785 27.545079356893993 30.93462348059979 21.526726686529436 24-25 20.367960606331582 27.97080475370454 32.01292385306153 19.64831078690235 26-27 21.211772102316303 26.48251224203404 33.06210493861702 19.243610717032638 28-29 19.490125578698304 27.002245396060324 34.0818535461952 19.42577547904617 30-31 19.412746366797762 29.111068930312626 32.64607280613065 18.83011189675896 32-33 19.1341976617818 28.826183962287438 31.98389217710157 20.05572619882919 34-35 19.85710312045835 27.481314940969863 32.21697546459591 20.44460647397588 36-37 21.059475759989994 27.258077259887937 32.71150338760971 18.970943592512363 38-39 21.64965620617534 26.553601330843122 32.99485241994421 18.801890043037325 40-41 19.61510079909584 27.617306336530994 33.80682893643169 18.96076392794147 42-43 19.11954105661986 29.08372348074308 32.15925640331408 19.637479059322978 44-45 19.45940178090902 29.260820166153074 30.861674247590354 20.418103805347553 46-47 19.64099778915203 28.681720182353814 29.943576746048727 21.733705282445428 48-49 20.445976022747285 28.52300649930245 30.224362262461657 20.806655215488608 50-51 21.19490948469998 29.006671454065387 30.333117370083297 19.46530169115134 52-53 19.76162484824513 29.36257194127189 30.91073220319271 19.96507100729027 54-55 18.67110186790839 29.060406255948816 31.446873314787194 20.8216185613556 56-57 20.0748730605927 29.99516956844579 29.29625193073254 20.633705440228965 58-59 19.849799652408997 28.755886444828704 30.09929055447787 21.295023348284428 60-61 19.96972231373312 28.281218755048908 30.85183217307272 20.897226758145255 62-63 21.580372861215817 27.899362691824713 30.386192186515093 20.134072260444377 64-65 20.665162949753856 28.44721341945281 31.091857024555132 19.795766606238203 66-67 19.530948042247374 28.731928653585083 31.026577837053605 20.710545467113942 68-69 19.253508547679054 30.003801772404948 30.419802351722648 20.32288732819335 70-71 19.56798183055013 29.733959096794017 29.200972109455574 21.49708696320028 72-73 19.726048514379478 28.871223546101938 28.76896558723443 22.633762352284155 74-75 19.899760317879167 29.529232276607104 29.262860901531738 21.30814650398199 76-77 20.659354231364063 28.04569151366862 30.391223979810984 20.903730275156338 78-79 19.634266332428517 27.985275721751684 31.273049604479365 21.107408341340435 80-81 19.28890108006172 30.00281158923367 29.95653694496828 20.75175038573633 82-83 19.295595966134844 29.51757375601233 29.273885070776423 21.912945207076408 84-85 19.448158122403434 28.477552348230045 29.143408558398676 22.930880970967845 86-87 20.28740571516438 28.312085208504374 29.855706789606494 21.544802286724753 88-89 20.957116830261675 27.094565265560107 30.23591427274607 21.712403631432153 90-91 19.70078493428409 27.799097354484825 30.91207067409849 21.588047037132593 92-93 19.073442273647668 29.26697069890659 30.110300833921706 21.549286193524036 94-95 19.00799605888738 29.217694081555905 29.281523209307696 22.492786650249023 96-97 19.11948767758381 28.249571478297696 28.690011419016457 23.94092942510203 98-99 19.869917343311897 27.90808413898963 28.841380021625586 23.380618496072888 100-101 20.61523370800016 27.54041198264557 29.60641467792066 22.237939631433612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14983.0 1 9395.0 2 6564.5 3 8378.0 4 5820.5 5 4811.0 6 8039.5 7 15446.0 8 20692.5 9 19660.5 10 14068.5 11 8828.0 12 7488.5 13 7027.5 14 6585.5 15 6128.5 16 5625.0 17 5422.5 18 5398.5 19 5412.5 20 5593.0 21 5950.0 22 6521.5 23 7218.0 24 7858.5 25 8716.0 26 10495.0 27 12395.5 28 13605.0 29 15503.0 30 18177.5 31 20980.0 32 24735.0 33 29418.0 34 33804.0 35 36953.5 36 42410.5 37 47656.0 38 48600.0 39 48701.0 40 47645.0 41 46019.0 42 45076.5 43 46066.5 44 50119.5 45 52964.0 46 55698.5 47 67895.5 48 127629.5 49 128240.0 50 76230.0 51 64200.0 52 50871.5 53 44973.5 54 40878.0 55 37253.0 56 34212.0 57 30716.5 58 27410.0 59 24470.5 60 21552.5 61 18530.5 62 15150.0 63 11516.5 64 8271.5 65 6016.5 66 4533.5 67 3479.5 68 2583.5 69 1936.5 70 1526.0 71 1198.0 72 968.5 73 901.0 74 800.0 75 594.5 76 386.5 77 243.0 78 171.0 79 115.0 80 74.0 81 53.0 82 40.0 83 27.5 84 13.5 85 7.0 86 3.0 87 1.5 88 0.5 89 0.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22843462485208527 2 0.0 3 0.0 4 0.0 5 0.029404649116406754 6 0.003126986939617882 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0026332521596782164 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.485941999329618E-4 26-27 0.0012617666598458122 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00702200575914191 36-37 0.01739043613787489 38-39 0.013001682538411195 40-41 0.008558069518954204 42-43 0.007268873149111744 44-45 0.003511002879570955 46-47 6.03453619926258E-4 48-49 0.0016457825997988854 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.001234336949849164 58-59 4.937347799396656E-4 60-61 0.0025509630296882725 62-63 9.874695598793312E-4 64-65 0.0013440557898357565 66-67 0.005513371709326266 68-69 3.840159399530733E-4 70-71 5.4859419993296175E-5 72-73 0.0 74-75 5.485941999329618E-4 76-77 0.058068696062904004 78-79 0.14164702242269073 80-81 0.001563493469808941 82-83 0.00128919636984246 84-85 0.0 86-87 0.10401346030728956 88-89 0.34630008870768214 90-91 0.38162955518336483 92-93 4.388753599463694E-4 94-95 3.01726809963129E-4 96-97 2.468673899698328E-4 98-99 0.0 100-101 0.0011794775298558677 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1822841.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.0706246512335 #Duplication Level Percentage of deduplicated Percentage of total 1 66.64140111098259 22.03872762373601 2 15.055124751577182 9.95764759473808 3 6.4308007366851285 6.380117921093693 4 3.460871218261173 4.578126921014899 5 2.0761069816420044 3.4329077362844025 6 1.3709851701662001 2.7203601578984338 7 0.9554325598406115 2.211772609623924 8 0.6538191946796226 1.7297767341617254 9 0.5019676245292686 1.4940344608090879 >10 2.514883638122238 14.761951403605366 >50 0.20246922795272385 4.629207855307775 >100 0.11550086931339959 6.947573160257684 >500 0.009486324404292514 2.0586549597995845 >1k 0.007988483708877907 5.199309113661303 >5k 0.0019971209272194768 4.334656418269059 >10k+ 0.0011649872075446947 7.525175329739013 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25005 1.371759796932371 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 23823 1.3069159625002948 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 21473 1.1779963255160488 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20261 1.1115067084841739 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17062 0.9360114239256194 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16025 0.8791222053925712 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13077 0.7173966352523342 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8632 0.4735465133821326 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 8368 0.4590636265039024 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8230 0.4514930265448276 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7358 0.40365561231067326 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7020 0.38511312835293915 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6739 0.369697631334823 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5656 0.3102848794820832 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5615 0.30803564326235805 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5537 0.30375660850288094 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5434 0.29810608824357143 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5127 0.28126424630562946 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5041 0.27654633618620605 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4616 0.25323108268905514 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4433 0.24319180883028194 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3865 0.21203165827408973 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3648 0.20012716413554446 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3621 0.19864595979572547 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3553 0.19491551923618133 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3421 0.18767407579706624 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2982 0.1635907904200092 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2890 0.15854372378062595 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2741 0.1503696702016248 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2694 0.1477912774619399 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2622 0.1438413992224226 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2500 0.13714854998324044 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2472 0.13561248622342817 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1982 0.10873137042671302 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1925 0.10560438348709514 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1900 0.10423289798726273 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1852 0.10159964582758452 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.485941999329618E-5 0.0 10-11 0.0 0.0 0.0 1.0971883998659236E-4 0.0 12-13 0.0 0.0 0.0 1.0971883998659236E-4 0.0 14-15 0.0 0.0 0.0 1.3714854998324045E-4 0.0 16-17 0.0 0.0 0.0 3.5658622995642515E-4 0.0 18-19 0.0 0.0 0.0 4.1144564994972135E-4 0.0 20-21 0.0 0.0 0.0 7.406021699094984E-4 0.0 22-23 0.0 0.0 0.0 0.002139517379738551 0.0 24-25 0.0 0.0 0.0 0.004361323889467046 0.0 26-27 0.0 0.0 0.0 0.007625459379068169 0.0 28-29 0.0 0.0 0.0 0.01349541731835086 0.0 30-31 0.0 0.0 0.0 0.029075492596446973 0.0 32-33 0.0 0.0 0.0 0.05340564536347383 0.0 34-35 0.0 0.0 0.0 0.09605884440826161 0.0 36-37 0.0 0.0 0.0 0.15654135495087065 0.0 38-39 0.0 0.0 0.0 0.234770887861311 0.0 40-41 0.0 0.0 0.0 0.32251853014058823 0.0 42-43 0.0 0.0 0.0 0.41043075067984536 0.0 44-45 0.0 0.0 0.0 0.5084096747878724 0.0 46-47 0.0 0.0 0.0 0.6358481074322994 0.0 48-49 0.0 0.0 0.0 0.7875069740037666 0.0 50-51 0.0 0.0 0.0 0.9405098963650697 0.0 52-53 0.0 0.0 0.0 1.0945551477062454 0.0 54-55 0.0 0.0 0.0 1.250630197587173 0.0 56-57 0.0 0.0 0.0 1.4354515835445878 0.0 58-59 0.0 0.0 0.0 1.6653674127364921 0.0 60-61 0.0 0.0 0.0 1.9690417321093832 0.0 62-63 0.0 0.0 0.0 2.246218951625512 0.0 64-65 0.0 0.0 0.0 2.4844459829464007 0.0 66-67 0.0 0.0 0.0 2.7591545285628314 0.0 68-69 0.0 0.0 0.0 3.0689731029749714 0.0 70-71 0.0 0.0 0.0 3.433651097380408 0.0 72-73 0.0 0.0 0.0 3.8166521380636054 0.0 74-75 0.0 0.0 0.0 4.192247157047708 0.0 76-77 0.0 0.0 0.0 4.566964425311917 0.0 78-79 0.0 0.0 0.0 4.9917957737400025 0.0 80-81 0.0 0.0 0.0 5.461200400912642 0.0 82-83 0.0 0.0 0.0 5.942317514253849 0.0 84-85 0.0 0.0 0.0 6.497494844586006 0.0 86-87 0.0 0.0 0.0 7.091156058043461 0.0 88-89 0.0 0.0 0.0 7.635443793506949 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1325 0.0 28.29722 2 TATCACG 120 5.418862E-6 27.685308 2 CGACCGT 65 2.9663916E-4 21.89765 18-19 GGAACGC 195 4.2717147E-7 21.89765 8 AGTACTT 15980 0.0 21.617414 12-13 GAGTACT 15510 0.0 21.614716 12-13 TATAACG 115 0.0037420818 20.635012 2 ATGGGAT 4560 0.0 20.29562 5 ATCACGC 165 6.365898E-5 20.13477 3 GTACTTT 16805 0.0 19.892605 14-15 CATGGGG 4425 0.0 19.734999 4 ATAGTAC 435 0.0 19.63884 3 CATATAG 345 5.2750693E-11 19.259346 3 GTATAGG 620 0.0 19.187325 1 ATGGGAG 3795 0.0 19.009222 5 TACTTTT 18140 0.0 18.96487 14-15 GTACATG 24215 0.0 18.963085 1 AGTACTC 755 0.0 18.858488 5 TACATGG 24325 0.0 18.672035 2 ACCTGGG 1960 0.0 18.645208 3 >>END_MODULE