FastQCFastQC Report
Thu 26 May 2016
SRR938693_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938693_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2713612
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128790.47460727620603094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106140.39113919012740217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66990.2468665380312292No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43560.16052405428631655No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31770.11707642802287137No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29260.10782676373777829No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29070.10712658994727323No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28330.1043995972895167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA215450.024.3571431
GTACATG155300.024.1451681
TACATGG156850.023.5317232
GGTATCA161300.023.4233591
GAGTACT110050.022.73858312-13
ACATGGG156900.022.5276933
TACCTGG21400.022.3618852
GTACTTT117150.021.15918214-15
ATCAACG252300.020.6762073
TATCAAC252900.020.6458872
TCAACGC253250.020.5051024
AGTACTT114050.020.48803512-13
CAACGCA256500.020.245295
GTATACG1656.424837E-520.1112611
CATGGGA94750.020.1023864
AACGCAG260950.019.9209216
GTACCTG26700.018.4649141
ACGCAGA281850.018.427617
CGCAGAG282800.018.2819988
ACTTTTT138650.018.20437816-17
Produced by FastQC (version 0.11.5)