##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938693_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2713612 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.618373592097914 33.0 31.0 34.0 30.0 34.0 2 31.683976559655544 33.0 31.0 34.0 30.0 34.0 3 31.692444977395443 33.0 31.0 34.0 28.0 34.0 4 35.240246947610785 37.0 35.0 37.0 33.0 37.0 5 35.26995089939166 37.0 35.0 37.0 33.0 37.0 6 35.35542922127408 37.0 35.0 37.0 33.0 37.0 7 35.27436899600975 37.0 35.0 37.0 33.0 37.0 8 35.24239500709755 37.0 35.0 37.0 32.0 37.0 9 36.808494361021395 39.0 37.0 39.0 33.0 39.0 10-11 36.69944174038146 39.0 37.0 39.0 32.0 39.0 12-13 36.581068516796066 39.0 37.0 39.0 32.0 39.0 14-15 37.73698819138477 40.0 38.0 41.0 32.0 41.0 16-17 37.66758714952617 40.0 37.0 41.0 32.0 41.0 18-19 37.614444511595615 40.0 37.0 41.0 32.0 41.0 20-21 37.513648598252075 40.0 37.0 41.0 32.0 41.0 22-23 37.422345014688915 40.0 37.0 41.0 32.0 41.0 24-25 37.42719777182589 40.0 37.0 41.0 32.0 41.0 26-27 37.326770002491145 40.0 37.0 41.0 31.0 41.0 28-29 37.20008239939977 40.0 37.0 41.0 31.0 41.0 30-31 36.978708452055784 40.0 36.5 41.0 30.5 41.0 32-33 36.745980633929975 39.5 36.0 41.0 30.0 41.0 34-35 36.41423976603877 39.0 35.0 41.0 30.0 41.0 36-37 36.165170628667624 39.0 35.0 41.0 29.5 41.0 38-39 35.978466707841804 39.0 35.0 41.0 29.0 41.0 40-41 35.779296929701076 39.0 35.0 41.0 27.5 41.0 42-43 35.495871185711145 39.0 35.0 40.0 26.5 41.0 44-45 35.42111620968657 39.0 35.0 41.0 26.0 41.0 46-47 35.1926076756736 39.0 35.0 41.0 24.5 41.0 48-49 34.9220059831693 39.0 35.0 41.0 24.0 41.0 50-51 33.75659139921256 38.0 33.0 40.0 22.0 40.5 52-53 33.83755304737744 38.0 33.5 39.5 22.5 40.5 54-55 34.336584227958895 38.0 34.0 40.0 23.0 41.0 56-57 34.22556301343007 38.0 34.0 40.0 22.0 41.0 58-59 33.990463080204535 38.0 34.0 40.0 22.0 41.0 60-61 33.68627202415084 37.5 33.5 40.0 20.5 41.0 62-63 33.3185809172424 37.0 33.0 40.0 19.0 41.0 64-65 32.912435897246915 36.0 33.0 39.5 18.0 41.0 66-67 32.50687662790406 36.0 32.0 39.0 14.0 41.0 68-69 32.1047865354369 35.0 32.0 39.0 12.0 40.5 70-71 31.699366748083364 35.0 31.0 38.0 8.0 40.0 72-73 31.28940154303563 35.0 31.0 37.0 7.0 40.0 74-75 30.842453158373416 35.0 31.0 37.0 4.0 39.0 76-77 30.420551832760175 35.0 30.0 36.0 2.0 39.0 78-79 30.020414119630956 34.5 30.0 36.0 2.0 38.5 80-81 29.612767411111093 34.0 29.5 35.5 2.0 37.0 82-83 29.186918210856966 34.0 29.0 35.0 2.0 37.0 84-85 28.806321242683183 34.0 29.0 35.0 2.0 36.0 86-87 28.447758743696596 34.0 29.0 35.0 2.0 36.0 88-89 28.09558127691063 34.0 28.5 35.0 2.0 36.0 90-91 27.710731305728306 34.0 27.0 35.0 2.0 35.0 92-93 27.294501019305635 34.0 26.5 35.0 2.0 35.0 94-95 26.81481656183714 33.5 25.0 35.0 2.0 35.0 96-97 26.26507142509688 33.0 24.5 35.0 2.0 35.0 98-99 25.60419046643367 33.0 21.5 35.0 2.0 35.0 100-101 23.446146317159567 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24139965937923336 1101 2 0.08421944710294227 1101 3 0.2325344640572844 1101 4 0.2367165657718715 1101 5 0.014067892408824889 1101 6 -0.02134050086975492 1101 7 0.18392365047214554 1101 8 0.2629019395060652 1101 9 0.260861386821162 1101 10-11 0.2974907196324281 1101 12-13 0.3358182717656746 1101 14-15 0.47797254623318963 1101 16-17 0.43881136674013277 1101 18-19 0.436462796148156 1101 20-21 0.5537005853924413 1101 22-23 0.45252812951596155 1101 24-25 0.3863832070061193 1101 26-27 0.4140858352545962 1101 28-29 0.41545672838271486 1101 30-31 0.29409991536771685 1101 32-33 0.27661806984944093 1101 34-35 0.2924469990059535 1101 36-37 0.3479436960884499 1101 38-39 0.3508062844022817 1101 40-41 0.3990519435986215 1101 42-43 0.26659476920481495 1101 44-45 0.4294092508106573 1101 46-47 0.451078877272586 1101 48-49 0.35664570060544065 1101 50-51 0.22278325656157705 1101 52-53 0.2073053495423096 1101 54-55 0.2945117719261816 1101 56-57 0.16797591592442984 1101 58-59 0.1891326675526841 1101 60-61 -2.4848629558960056E-4 1101 62-63 0.08159739935616983 1101 64-65 0.085835250129918 1101 66-67 -0.04566903409719458 1101 68-69 -0.05803485410206832 1101 70-71 -0.15047099339772174 1101 72-73 -0.27812497073416864 1101 74-75 -0.25542015068030466 1101 76-77 -0.3280208449844402 1101 78-79 -0.37814158322889924 1101 80-81 -0.1767277388050701 1101 82-83 -0.403407970451493 1101 84-85 -0.35227040706758217 1101 86-87 -0.362810620440964 1101 88-89 -0.37593713452099564 1101 90-91 -0.5037126794692455 1101 92-93 -0.6433932669792561 1101 94-95 -0.8924968406714271 1101 96-97 -1.0099539605862304 1101 98-99 -1.0216945420646546 1101 100-101 -0.8034774253485502 1102 1 0.2757695808967462 1102 2 0.37414512446504133 1102 3 0.3004728428254104 1102 4 0.301527985944368 1102 5 0.3356184068435013 1102 6 0.2767761967646791 1102 7 0.34375171004118954 1102 8 0.3170247659848968 1102 9 0.5197511230867207 1102 10-11 0.37581943091116443 1102 12-13 0.3463193949249401 1102 14-15 0.2705916565801161 1102 16-17 0.41169913401044056 1102 18-19 0.5052438910204486 1102 20-21 0.5270344271517828 1102 22-23 0.44959931840083556 1102 24-25 0.33533998617360083 1102 26-27 0.334501958829847 1102 28-29 0.4562918180967941 1102 30-31 0.5020790606573797 1102 32-33 0.40134345047563613 1102 34-35 0.4693973442734318 1102 36-37 0.49302980496556614 1102 38-39 0.43965428351484803 1102 40-41 0.40225780066619876 1102 42-43 0.34793680586695075 1102 44-45 0.6217604668732193 1102 46-47 0.48852382860234655 1102 48-49 0.5330325943074286 1102 50-51 0.565269529059421 1102 52-53 0.6168214897569584 1102 54-55 0.5150036740865289 1102 56-57 0.2990283577558728 1102 58-59 0.35042755925795177 1102 60-61 0.28051318208124343 1102 62-63 0.28791163435991507 1102 64-65 0.2312363982482779 1102 66-67 0.35051998944754814 1102 68-69 0.40115688721077447 1102 70-71 0.2819606464148059 1102 72-73 0.29103668100326274 1102 74-75 0.35054139844751475 1102 76-77 0.3742138953023151 1102 78-79 0.1138132685418114 1102 80-81 -0.03975111715721624 1102 82-83 0.04861517217586808 1102 84-85 -0.11525315755983101 1102 86-87 -0.05455158663525239 1102 88-89 -0.17524736220340031 1102 90-91 -0.21960434313157506 1102 92-93 -0.36082123225838103 1102 94-95 -0.40619141068168574 1102 96-97 -0.33538873415177406 1102 98-99 -0.474519426282189 1102 100-101 -0.2621450950010633 1103 1 0.20662662623794859 1103 2 0.2756243145480255 1103 3 0.22479193507101058 1103 4 0.2300632323931069 1103 5 0.1384748279899597 1103 6 0.2617200679187661 1103 7 0.2212126545981974 1103 8 0.18018453820249647 1103 9 0.34158179322522386 1103 10-11 0.26064968672282873 1103 12-13 0.35595871421731573 1103 14-15 0.43112214367918966 1103 16-17 0.5055625532778265 1103 18-19 0.4796376405268816 1103 20-21 0.39288164440076656 1103 22-23 0.538679318097941 1103 24-25 0.5048553076122531 1103 26-27 0.45418873098578416 1103 28-29 0.5478233642715153 1103 30-31 0.4312264668956516 1103 32-33 0.39564233211771693 1103 34-35 0.46397370589962605 1103 36-37 0.53305291207068 1103 38-39 0.5246396955320591 1103 40-41 0.6746230660138721 1103 42-43 0.6444357305644033 1103 44-45 0.5409114944145941 1103 46-47 0.5442286791471957 1103 48-49 0.4598461667026541 1103 50-51 0.4987553997569947 1103 52-53 0.4699092929701081 1103 54-55 0.5149315319951384 1103 56-57 0.5057028061224145 1103 58-59 0.4354196648712616 1103 60-61 0.4224026801399603 1103 62-63 0.49631843948891685 1103 64-65 0.3244009790371436 1103 66-67 0.3323501890059646 1103 68-69 0.28049271479888205 1103 70-71 0.21395147875739795 1103 72-73 0.30905394858807966 1103 74-75 0.22925458637340412 1103 76-77 0.174718430179869 1103 78-79 0.2879365561982219 1103 80-81 0.1858514687615127 1103 82-83 0.2306909252933238 1103 84-85 0.38309751331428643 1103 86-87 0.26337225457479363 1103 88-89 0.22628071141479467 1103 90-91 0.20214488149306575 1103 92-93 0.12968914159856837 1103 94-95 0.16309751846193166 1103 96-97 0.07498421679647294 1103 98-99 0.08494086973973225 1103 100-101 0.2530104912173208 1104 1 0.12587199417371053 1104 2 0.2487380973386557 1104 3 0.19789106538609502 1104 4 0.2739245441579996 1104 5 0.10929381802842641 1104 6 0.20143897697877833 1104 7 0.1880528926081695 1104 8 0.27450574950248097 1104 9 0.26175282122139976 1104 10-11 0.2793955799968799 1104 12-13 0.3578720721130466 1104 14-15 0.4267248551130649 1104 16-17 0.4048617347824788 1104 18-19 0.31726683772178177 1104 20-21 0.33934459057536515 1104 22-23 0.3860483787371436 1104 24-25 0.24680666246886318 1104 26-27 0.3149734100347388 1104 28-29 0.48270426787489384 1104 30-31 0.3868066807303876 1104 32-33 0.2929582540084539 1104 34-35 0.35505499944705576 1104 36-37 0.39532797640713824 1104 38-39 0.3878070149732338 1104 40-41 0.281730175341238 1104 42-43 0.3296747460470897 1104 44-45 0.5618000097773788 1104 46-47 0.5433532273401269 1104 48-49 0.5515632701475184 1104 50-51 0.5060514588056648 1104 52-53 0.6726279724298578 1104 54-55 0.6905735120634731 1104 56-57 0.6238026327661572 1104 58-59 0.5774770283720301 1104 60-61 0.5315860049237742 1104 62-63 0.47099123223462414 1104 64-65 0.23835061968854632 1104 66-67 0.41477392623142606 1104 68-69 0.4747171928750191 1104 70-71 0.4212722453749187 1104 72-73 0.29476119804150613 1104 74-75 0.24122397856183042 1104 76-77 0.21940189369171037 1104 78-79 0.12683594826835787 1104 80-81 0.2113217056853216 1104 82-83 0.12828033765646651 1104 84-85 0.23120242396376156 1104 86-87 0.39464223705634893 1104 88-89 0.45312428857607756 1104 90-91 0.3468083692446733 1104 92-93 0.23861483822295781 1104 94-95 0.25680325857594966 1104 96-97 0.2170570393310669 1104 98-99 0.3446106298262812 1104 100-101 0.5754729647487657 1105 1 0.19470767478836493 1105 2 0.1302726829861527 1105 3 0.11261512655434203 1105 4 0.0803477429871009 1105 5 0.04634613025400114 1105 6 0.03737483626467508 1105 7 0.1792534147288336 1105 8 0.21332821555333226 1105 9 0.1768173431869755 1105 10-11 0.19928228692112526 1105 12-13 0.12898932471153302 1105 14-15 0.20723890649155408 1105 16-17 0.21467581006994862 1105 18-19 0.19740727073298814 1105 20-21 0.289638525769071 1105 22-23 0.3321966080179024 1105 24-25 0.22275551015243167 1105 26-27 0.264418414509592 1105 28-29 0.2835237969970166 1105 30-31 0.26428461337810916 1105 32-33 0.20094074713462362 1105 34-35 0.22634224012335835 1105 36-37 0.24093970732113945 1105 38-39 0.073240421972109 1105 40-41 -0.016894936530221116 1105 42-43 0.11329030239961924 1105 44-45 0.10515201797105789 1105 46-47 -0.03169374251838519 1105 48-49 -0.01600507241015947 1105 50-51 -0.04610265240991396 1105 52-53 -0.10226100423302853 1105 54-55 -0.07434800021250254 1105 56-57 -0.12554774541592195 1105 58-59 -0.05334432623384089 1105 60-61 0.05236441219727794 1105 62-63 -0.07603254267192483 1105 64-65 -0.062218511219640504 1105 66-67 -0.10120652816863185 1105 68-69 -0.13274443157935778 1105 70-71 -0.11581314364697803 1105 72-73 -0.10659159850267486 1105 74-75 -0.19375305749874272 1105 76-77 -0.15969575191888907 1105 78-79 -0.11262309466120612 1105 80-81 -0.2899426033609984 1105 82-83 -0.12059044717230805 1105 84-85 -0.30498334889436407 1105 86-87 -0.43105334494987346 1105 88-89 -0.44716027687801585 1105 90-91 -0.24444724048305488 1105 92-93 -0.2769211593473706 1105 94-95 -0.15179230630626606 1105 96-97 -0.14974807331581985 1105 98-99 -0.27180697139113974 1105 100-101 -0.216355854954827 1106 1 0.03796062549816881 1106 2 0.1879151660600904 1106 3 0.18231006941763184 1106 4 0.22436987707762057 1106 5 0.14580659246275474 1106 6 0.0645498754879128 1106 7 0.11259032168996441 1106 8 0.2383385430709879 1106 9 0.17645678014324773 1106 10-11 0.20509576595178913 1106 12-13 0.18951917903819293 1106 14-15 0.2727875076962931 1106 16-17 0.2694235879535185 1106 18-19 0.3218906614977257 1106 20-21 0.29432297843464283 1106 22-23 0.2786098591364592 1106 24-25 0.1937836562050066 1106 26-27 0.045139745947302856 1106 28-29 0.21121063839274967 1106 30-31 0.22311208742863897 1106 32-33 0.21170896776934 1106 34-35 0.27370134251339095 1106 36-37 0.40983429090150025 1106 38-39 0.3378780947830151 1106 40-41 0.4728024812036651 1106 42-43 0.4701719761161911 1106 44-45 0.35480612280262847 1106 46-47 0.32684760128834256 1106 48-49 0.4819624267784377 1106 50-51 0.595876379995687 1106 52-53 0.4327131443034844 1106 54-55 0.5070167042391418 1106 56-57 0.535927760165734 1106 58-59 0.3281221066002189 1106 60-61 0.2962454413274074 1106 62-63 0.23176888683253338 1106 64-65 0.2682693209777298 1106 66-67 0.20136413698750744 1106 68-69 0.44322256143419736 1106 70-71 0.433534522600727 1106 72-73 0.37705378721043914 1106 74-75 0.24403821650223634 1106 76-77 0.06466526876254974 1106 78-79 0.28016040403242215 1106 80-81 0.031039578904152876 1106 82-83 0.17327690083049774 1106 84-85 0.15850224231835242 1106 86-87 0.3929231507133686 1106 88-89 0.37504127252965347 1106 90-91 0.355990835614417 1106 92-93 0.3973089305453712 1106 94-95 0.5013082425122874 1106 96-97 0.6229472634465942 1106 98-99 0.5962938934534598 1106 100-101 0.7853815548744087 1107 1 0.11171837493159487 1107 2 0.11880516413699382 1107 3 0.09067846826947346 1107 4 0.1699186732164435 1107 5 0.014319273689558543 1107 6 -0.03599378843603773 1107 7 -0.09893132264477345 1107 8 0.026131284422923784 1107 9 0.10806206995181356 1107 10-11 0.09093300285488937 1107 12-13 0.15917269979563287 1107 14-15 0.1840673272364839 1107 16-17 0.07979589842099699 1107 18-19 0.1397815150756756 1107 20-21 -0.01273812354461512 1107 22-23 -0.015360071967087663 1107 24-25 -0.016230375278389886 1107 26-27 -0.06366037360652399 1107 28-29 -0.28516423198288265 1107 30-31 -0.01057588445880242 1107 32-33 0.017841562253664733 1107 34-35 -0.09076742774144719 1107 36-37 0.02353230562729891 1107 38-39 0.03917092226540575 1107 40-41 0.07167642762234294 1107 42-43 0.10507048330248381 1107 44-45 0.10963444091606078 1107 46-47 0.12284364271643966 1107 48-49 0.18206268369254985 1107 50-51 0.37836187565209656 1107 52-53 0.17914868851215004 1107 54-55 0.16841226571084178 1107 56-57 0.09110157618842862 1107 58-59 0.06704108619297244 1107 60-61 0.03813987888229775 1107 62-63 0.03797633436087722 1107 64-65 0.06444435021328587 1107 66-67 0.09989335051481874 1107 68-69 0.019321727274515865 1107 70-71 0.09349804971308728 1107 72-73 -0.08984619672418503 1107 74-75 0.008603299872039116 1107 76-77 -0.03185690712772882 1107 78-79 0.019108005846412368 1107 80-81 0.03130100398476188 1107 82-83 -0.07734883104761181 1107 84-85 -0.05785675525495648 1107 86-87 0.07384757459553981 1107 88-89 0.03184468218803005 1107 90-91 0.12627014100982237 1107 92-93 0.07545891151225348 1107 94-95 0.07746768648673097 1107 96-97 0.2940240604274642 1107 98-99 0.4055837695495761 1107 100-101 0.565438712263127 1108 1 0.0492880516317058 1108 2 0.010226490310309089 1108 3 0.01394852338276209 1108 4 -0.041023512786978245 1108 5 0.012368718631222464 1108 6 0.1415377329105496 1108 7 0.15714998123200274 1108 8 0.1980949504772198 1108 9 0.12382156561027102 1108 10-11 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-2.1822698994796816 2316 88-89 -2.2725875890953446 2316 90-91 -2.4187295273298837 2316 92-93 -2.522308646215066 2316 94-95 -2.4213670803560845 2316 96-97 -2.356756999558826 2316 98-99 -2.6457504261005163 2316 100-101 -2.884608457200546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10989.0 3 3520.0 4 3493.0 5 3870.0 6 4341.0 7 5000.0 8 6042.0 9 7353.0 10 8826.0 11 11030.0 12 13795.0 13 17044.0 14 18760.0 15 18544.0 16 22788.0 17 28974.0 18 24652.0 19 17851.0 20 17767.0 21 19080.0 22 20434.0 23 22948.0 24 26171.0 25 29907.0 26 34492.0 27 39966.0 28 46998.0 29 55483.0 30 66273.0 31 79451.0 32 99004.0 33 126382.0 34 170006.0 35 241669.0 36 355330.0 37 514100.0 38 445823.0 39 75431.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86511884733696 18.301544989543324 14.17154558748949 22.661790575630224 2 15.627017165688098 20.093017388475403 40.24848161598993 24.031483829846565 3 19.74870375930088 24.86237239525512 29.389496264156485 25.999427581287517 4 13.059654693625806 16.278144122104063 36.53239479015578 34.12980639411436 5 13.933489602377316 37.44343302894937 33.38275006705942 15.24032730161389 6 31.910150550287515 35.20214319788305 17.541663256631505 15.346042995197934 7 28.832508868313926 32.87631992437878 20.237931092961166 18.053240114346135 8 26.66363857906498 34.29135929549773 19.428414055094727 19.616588070342573 9 27.25185658462166 16.759457493493706 20.074193335503633 35.914492586381 10-11 25.547344744329127 26.26578253385454 26.44999120709125 21.73688151472508 12-13 26.865726945530245 23.751536522220672 27.037002625821536 22.345733906427547 14-15 23.663024811658065 24.945748231978566 25.956106761875482 25.435120194487887 16-17 22.396058669432254 28.26252261157023 26.588994513497077 22.75242420550044 18-19 22.33203970979376 26.631174975426557 29.164746386616713 21.87203892816297 20-21 23.495340434659255 25.609515317084753 28.903573885049365 21.991570363206627 22-23 22.952933044403828 25.678969279084257 28.867918370838797 22.50017930567312 24-25 22.79452252842354 25.807176153104837 29.16763941245048 22.23066190602114 26-27 22.653347550160603 25.814366396554323 29.224007603617547 22.308278449667526 28-29 22.291429955934465 26.199060864575465 29.242861246852925 22.266647932637152 30-31 22.471410453282616 25.92335842525788 29.091231567999763 22.513999553459737 32-33 22.32327206066702 26.13171804348275 28.90502638401798 22.639983511832252 34-35 22.873060584678946 26.015082503578796 28.78390430819373 22.327952603548525 36-37 22.97453412285353 25.68650415149783 28.933555299491392 22.405406426157253 38-39 22.759551392015943 25.881897610587956 28.9768499563549 22.381701041041204 40-41 22.623022836530676 26.04297876931179 28.965767502211783 22.36823089194575 42-43 22.62892405837685 26.21157063772468 28.39480692373148 22.76469838016699 44-45 22.603396655548856 26.241784516876926 28.368225437133216 22.786593390441006 46-47 22.861356222247974 26.383218514814715 27.971283214034877 22.784142048902435 48-49 23.049574611873837 26.371379109230787 27.739601366426275 22.839444912469105 50-51 22.872262237248272 26.711100562761057 27.679650146938435 22.736987053052236 52-53 23.00682772428569 26.730027730802743 27.553064922071226 22.710079622840347 54-55 22.61176924912532 26.59271075869446 27.724357223956147 23.071162768224074 56-57 22.818148115449375 26.849666278168222 27.369928443361314 22.962257163021093 58-59 22.974973973243053 26.436282835071594 27.48523608387333 23.103507107812025 60-61 22.74951820249295 26.416710354326966 27.987690254056847 22.846081189123236 62-63 22.807905690268964 26.44908255174095 27.919298685303335 22.82371307268675 64-65 22.656128481763655 26.52519505875835 27.884662389471643 22.934014070006356 66-67 22.631265908620403 26.603805588279627 27.579904651048242 23.185023852051728 68-69 22.542958024788298 26.56419508404561 27.74383005611683 23.14901683504926 70-71 22.661037228698728 26.47329686754333 27.55665935239735 23.309006551360596 72-73 22.713390133180656 26.438334045615168 27.52782898717967 23.320446834024505 74-75 22.51663305154399 26.94971877538377 27.328955868556076 23.204692304516165 76-77 22.727621931973747 26.529128258006235 27.41572617706597 23.327523632954055 78-79 22.664557581666433 26.59947428335805 27.459265103434184 23.27670303154133 80-81 22.684218981701182 26.75929099762131 27.298734844452277 23.257755176225235 82-83 22.682815676277414 26.63722147789649 27.36264598728628 23.31731685853981 84-85 22.578429331001068 26.563473222920976 27.331232444388913 23.52686500168904 86-87 22.730200173903288 26.518918600709796 27.31695956924411 23.433921656142807 88-89 22.751818623975897 26.37556957260786 27.226454718665245 23.646157084750996 90-91 22.657046154757673 26.402010013034722 27.36804197772248 23.572901854485128 92-93 22.780841478308332 26.42913853234179 27.28014862774113 23.50987136160875 94-95 22.61986542971723 26.26223984825384 27.333399005073144 23.78449571695579 96-97 22.64696378989976 25.9958726540649 27.38228315000375 23.97488040603159 98-99 22.677754931812355 26.108624387949796 27.143395412435506 24.07022526780234 100-101 22.980915482215085 25.62153161919234 27.456024596901397 23.941528301691186 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3191.0 1 1949.5 2 1038.5 3 1366.0 4 1100.5 5 1000.5 6 1704.5 7 3259.5 8 5033.5 9 5729.5 10 4979.5 11 4051.0 12 3935.0 13 4197.5 14 4487.0 15 4788.0 16 5006.5 17 5134.5 18 5338.0 19 5566.0 20 5947.5 21 6530.5 22 7343.5 23 8515.5 24 9771.5 25 11514.0 26 14211.5 27 17576.0 28 20439.0 29 23313.0 30 27063.5 31 31267.5 32 35981.0 33 39933.5 34 43857.5 35 48019.0 36 53235.0 37 58780.5 38 62383.5 39 64827.5 40 67635.0 41 71977.0 42 76204.0 43 80257.0 44 85195.0 45 90350.5 46 93884.5 47 99156.5 48 113889.0 49 115013.5 50 103508.5 51 99438.5 52 97051.0 53 97615.5 54 96460.0 55 91563.5 56 84957.5 57 77332.5 58 71603.5 59 65075.5 60 55680.5 61 46562.5 62 38792.5 63 30613.5 64 23143.0 65 18019.5 66 14119.0 67 11273.5 68 8652.5 69 6255.5 70 4676.5 71 3647.5 72 2932.0 73 2469.5 74 1910.0 75 1432.5 76 1059.0 77 713.5 78 473.0 79 342.0 80 259.5 81 173.5 82 111.5 83 85.0 84 67.5 85 49.0 86 32.5 87 23.5 88 19.0 89 17.5 90 15.0 91 10.5 92 7.0 93 7.5 94 8.0 95 5.0 96 4.0 97 3.5 98 3.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15986073174794332 2 0.09234923784240341 3 0.0851263924245618 4 0.1011198358497825 5 0.1226778183469118 6 0.03795678969580028 7 0.0422315349430943 8 0.045290188870037426 9 0.04565870139135588 10-11 0.04418465130608208 12-13 0.048569950309771626 14-15 0.04635887518186093 16-17 0.0452717632439715 18-19 0.049159570343881145 20-21 0.051425922349989606 22-23 0.0649503318823767 24-25 0.08162552347203653 26-27 0.08368919359141984 28-29 0.07256011544760269 30-31 0.06082299164361007 32-33 0.0502651079078365 34-35 0.29823718350302103 36-37 0.47538115250079965 38-39 0.4524596736747921 40-41 0.42760350411186265 42-43 0.4583742996419532 44-45 0.4676608151791782 46-47 0.46738443078818936 48-49 0.48411489925604695 50-51 0.47246990358238394 52-53 0.4792505339746434 54-55 0.47053521284546207 56-57 0.44905093285259645 58-59 0.42692175594742354 60-61 0.39478746408845483 62-63 0.4548365794372961 64-65 0.4938067785667221 66-67 0.4947280598700182 68-69 0.5051938154754622 70-71 0.5025958022001672 72-73 0.4927012410027668 74-75 0.4860864412451006 76-77 0.4668869388844094 78-79 0.44098050863572247 80-81 0.4468582833507517 82-83 0.4582453202594918 84-85 0.4560158195055152 86-87 0.4674765589185189 88-89 0.4795269183656322 90-91 0.4839306429953877 92-93 0.48547839558492517 94-95 0.4906007196312516 96-97 0.4912087652914271 98-99 0.48494405242901345 100-101 0.4711064072535057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2713612.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.676291717920954 #Duplication Level Percentage of deduplicated Percentage of total 1 72.89130969031558 37.66752583258066 2 14.513487491356889 15.000064268955104 3 5.192383369595617 8.049693531555134 4 2.4588447148305907 5.082559070906151 5 1.3850871402961833 3.5788083558343238 6 0.8563059284046064 2.655042897361294 7 0.525380680817075 1.900480772740416 8 0.3752053944985694 1.5511378736196548 9 0.2677305121291542 1.2451788041917096 >10 1.3981497726513255 12.897931447000479 >50 0.09059163591430862 3.2515410302879197 >100 0.04109768630557048 3.7469933214082483 >500 0.0031410201116095905 1.1031604057475983 >1k 0.001070802310756549 1.1560976443032354 >5k 7.138682071710326E-5 0.24712652347513878 >10k+ 1.4277364143420651E-4 0.866658220033055 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12879 0.47460727620603094 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10614 0.39113919012740217 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6699 0.2468665380312292 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4356 0.16052405428631655 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3177 0.11707642802287137 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2926 0.10782676373777829 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2907 0.10712658994727323 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2833 0.1043995972895167 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.370250426368987E-5 2 0.0 0.0 0.0 0.0 7.370250426368987E-5 3 0.0 0.0 0.0 0.0 7.370250426368987E-5 4 0.0 0.0 0.0 0.0 7.370250426368987E-5 5 0.0 0.0 0.0 0.0 7.370250426368987E-5 6 0.0 0.0 0.0 0.0 7.370250426368987E-5 7 0.0 0.0 0.0 0.0 7.370250426368987E-5 8 0.0 0.0 0.0 0.0 7.370250426368987E-5 9 0.0 0.0 0.0 3.685125213184493E-5 7.370250426368987E-5 10-11 0.0 0.0 0.0 3.685125213184493E-5 7.370250426368987E-5 12-13 0.0 0.0 0.0 3.685125213184493E-5 7.370250426368987E-5 14-15 0.0 0.0 0.0 3.685125213184493E-5 1.842562606592247E-4 16-17 0.0 0.0 0.0 3.685125213184493E-5 1.842562606592247E-4 18-19 0.0 0.0 0.0 3.685125213184493E-5 2.5795876492291453E-4 20-21 0.0 0.0 0.0 7.370250426368987E-5 2.5795876492291453E-4 22-23 0.0 0.0 0.0 3.500868952525269E-4 2.5795876492291453E-4 24-25 0.0 0.0 0.0 7.554506687028212E-4 2.5795876492291453E-4 26-27 0.0 0.0 0.0 0.0015477525895374873 2.5795876492291453E-4 28-29 1.8425626065922467E-5 0.0 0.0 0.0040904889866347875 2.5795876492291453E-4 30-31 3.685125213184493E-5 0.0 0.0 0.01171869817792669 2.5795876492291453E-4 32-33 3.685125213184493E-5 0.0 0.0 0.02664345529132389 2.5795876492291453E-4 34-35 3.685125213184493E-5 0.0 0.0 0.049915021012583966 2.5795876492291453E-4 36-37 3.685125213184493E-5 0.0 0.0 0.08989862957563571 2.5795876492291453E-4 38-39 3.685125213184493E-5 0.0 0.0 0.14583883031177636 3.3166126918660445E-4 40-41 3.685125213184493E-5 0.0 0.0 0.20750940075441882 3.685125213184494E-4 42-43 3.685125213184493E-5 0.0 0.0 0.26927209932739093 3.869381473843718E-4 44-45 3.685125213184493E-5 0.0 0.0 0.34918403957529665 4.053637734502943E-4 46-47 5.52768781977674E-5 0.0 0.0 0.44895880472226685 4.237893995162168E-4 48-49 7.370250426368987E-5 0.0 0.0 0.55418755518475 5.52768781977674E-4 50-51 7.370250426368987E-5 0.0 0.0 0.6667128535693385 8.107275469005886E-4 52-53 7.370250426368987E-5 0.0 0.0 0.7798461976141025 8.475787990324335E-4 54-55 7.370250426368987E-5 0.0 0.0 0.9037401072813652 9.212813032961234E-4 56-57 7.370250426368987E-5 0.0 0.0 1.0623847477089576 0.0010134094336257359 58-59 7.370250426368987E-5 0.0 0.0 1.2379072616129352 0.0010318350596916581 60-61 7.370250426368987E-5 0.0 0.0 1.429460070194265 0.0010502606857575806 62-63 9.212813032961233E-5 0.0 0.0 1.6233160820338353 0.0011976656942849604 64-65 1.1055375639553481E-4 0.0 0.0 1.8198069584008325 0.0012529425724827278 66-67 1.1055375639553481E-4 0.0 0.0 2.0538861119423117 0.0012529425724827278 68-69 1.1055375639553481E-4 0.0 0.0 2.319233552917661 0.0012529425724827278 70-71 1.1055375639553481E-4 0.0 0.0 2.615480768805562 0.0012529425724827278 72-73 1.1055375639553481E-4 0.0 0.0 2.9125755634924966 0.0012529425724827278 74-75 1.1055375639553481E-4 0.0 0.0 3.211402367029627 0.0012529425724827278 76-77 1.1055375639553481E-4 0.0 0.0 3.539341659750915 0.0012529425724827278 78-79 1.1055375639553481E-4 0.0 0.0 3.9135292738976686 0.0012529425724827278 80-81 1.1055375639553481E-4 0.0 0.0 4.324586565802333 0.0012529425724827278 82-83 1.1055375639553481E-4 0.0 0.0 4.745372588269804 0.0012529425724827278 84-85 1.1055375639553481E-4 0.0 0.0 5.169806884698328 0.0012529425724827278 86-87 1.1055375639553481E-4 0.0 0.0 5.608410487571547 0.0012529425724827278 88-89 1.1055375639553481E-4 0.0 0.0 6.08137788305771 0.0012529425724827278 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 21545 0.0 24.357143 1 GTACATG 15530 0.0 24.145168 1 TACATGG 15685 0.0 23.531723 2 GGTATCA 16130 0.0 23.423359 1 GAGTACT 11005 0.0 22.738583 12-13 ACATGGG 15690 0.0 22.527693 3 TACCTGG 2140 0.0 22.361885 2 GTACTTT 11715 0.0 21.159182 14-15 ATCAACG 25230 0.0 20.676207 3 TATCAAC 25290 0.0 20.645887 2 TCAACGC 25325 0.0 20.505102 4 AGTACTT 11405 0.0 20.488035 12-13 CAACGCA 25650 0.0 20.24529 5 GTATACG 165 6.424837E-5 20.111261 1 CATGGGA 9475 0.0 20.102386 4 AACGCAG 26095 0.0 19.920921 6 GTACCTG 2670 0.0 18.464914 1 ACGCAGA 28185 0.0 18.42761 7 CGCAGAG 28280 0.0 18.281998 8 ACTTTTT 13865 0.0 18.204378 16-17 >>END_MODULE