FastQCFastQC Report
Thu 26 May 2016
SRR938693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938693_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2713612
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119960.4420676205736118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109460.4033738058351746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70120.2584009799484967No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43300.15956592173088857No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32760.12072470198392402No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31220.11504960915561988No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30390.11199095522867676No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29900.11018524387421637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG22350.024.840472
GTACATG157350.023.9409051
GGTATCA169500.023.7139321
TACATGG159250.023.5098082
ACATGGG158650.022.701433
GTATCAA222050.022.4557061
GAGTACT119150.022.16392112-13
GTACTTT126350.020.73191314-15
GTACCTG28350.020.66251
AGTACTT121850.019.92028612-13
CATGGGG63900.019.8272234
CATGGGA94950.019.5403944
TATCAAC262700.018.930092
ATCAACG262400.018.8793983
TCAACGC262900.018.8615424
CAACGCA264800.018.7082885
ACTTTTT144850.018.37840716-17
AACGCAG270550.018.3282186
ATGGGAG34400.018.2081995
TACTTTT139900.017.26535814-15
Produced by FastQC (version 0.11.5)