##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938693_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2713612 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01983297538484 33.0 31.0 34.0 30.0 34.0 2 32.24145050950541 34.0 31.0 34.0 30.0 34.0 3 32.323961568566176 34.0 31.0 34.0 30.0 34.0 4 35.95002712252157 37.0 35.0 37.0 35.0 37.0 5 35.857912627155244 37.0 35.0 37.0 35.0 37.0 6 35.89077841636903 37.0 35.0 37.0 35.0 37.0 7 35.828872366425266 37.0 35.0 37.0 33.0 37.0 8 35.80098591839953 37.0 35.0 37.0 33.0 37.0 9 37.451082910895146 39.0 37.0 39.0 34.0 39.0 10-11 37.37505675092828 39.0 37.0 39.0 34.0 39.0 12-13 37.3001036994235 39.0 37.0 39.0 34.0 39.0 14-15 38.51103013253184 40.0 38.0 41.0 34.0 41.0 16-17 38.43751667519159 40.0 38.0 41.0 33.0 41.0 18-19 38.46924394497076 40.0 38.0 41.0 33.5 41.0 20-21 38.546722781296666 40.0 38.0 41.0 34.0 41.0 22-23 38.49846772493636 40.0 38.0 41.0 34.0 41.0 24-25 38.42347247874788 40.0 38.0 41.0 34.0 41.0 26-27 38.2562385484734 40.0 38.0 41.0 33.5 41.0 28-29 38.08497732910969 40.0 37.5 41.0 33.0 41.0 30-31 37.84698383556676 40.0 37.0 41.0 33.0 41.0 32-33 37.60907989056652 40.0 36.5 41.0 32.0 41.0 34-35 37.56604002340792 40.0 37.0 41.0 32.0 41.0 36-37 37.472434157867816 40.0 36.5 41.0 32.0 41.0 38-39 37.362121408661224 40.0 36.0 41.0 32.0 41.0 40-41 37.191531434855094 40.0 36.0 41.0 31.0 41.0 42-43 36.99486219842778 40.0 36.0 41.0 31.0 41.0 44-45 36.7142557594822 39.5 35.5 41.0 30.5 41.0 46-47 36.38330645648678 39.0 35.0 41.0 30.0 41.0 48-49 36.06906127331395 39.0 35.0 41.0 29.0 41.0 50-51 35.80578634675849 39.0 35.0 41.0 28.0 41.0 52-53 35.57467961521397 39.0 35.0 40.5 28.0 41.0 54-55 35.311818343963694 38.0 35.0 40.0 27.0 41.0 56-57 35.04172059233228 38.0 34.0 40.0 27.0 41.0 58-59 34.73249399693103 38.0 34.0 40.0 26.0 41.0 60-61 34.37210938778278 37.0 34.0 40.0 26.0 41.0 62-63 33.99052591158943 37.0 33.0 40.0 25.5 41.0 64-65 33.60849045478867 36.0 33.0 39.0 24.5 41.0 66-67 33.20285766719782 36.0 33.0 39.0 23.0 40.0 68-69 32.75327404949566 35.0 32.0 38.5 22.5 40.0 70-71 32.281864356437104 35.0 31.5 37.5 21.5 40.0 72-73 31.927299665538037 35.0 31.0 37.0 20.5 39.0 74-75 31.50821248579384 35.0 31.0 37.0 19.5 39.0 76-77 29.503037280200708 32.5 28.5 35.0 16.0 37.0 78-79 30.55982229589197 34.0 30.5 35.5 17.5 37.5 80-81 30.622362740141185 34.0 31.0 35.0 17.5 37.0 82-83 30.333033978328515 34.0 31.0 35.0 13.5 37.0 84-85 29.96303616729289 34.0 30.0 35.0 8.5 36.0 86-87 29.43829958004313 34.0 29.5 35.0 4.0 36.0 88-89 28.92865873971666 34.0 29.0 35.0 2.0 35.5 90-91 28.51896420711583 34.0 29.0 35.0 2.0 35.0 92-93 28.232726159819457 34.0 29.0 35.0 2.0 35.0 94-95 27.850240564973916 33.5 27.5 35.0 2.0 35.0 96-97 27.327830213014977 33.0 27.0 35.0 2.0 35.0 98-99 26.600571489218062 33.0 25.5 35.0 2.0 35.0 100-101 23.46621587758309 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.001187064301497287 1101 2 0.11736957917699442 1101 3 0.05596659473611254 1101 4 0.044886239466571 1101 5 0.02849605405265976 1101 6 0.059884056697541155 1101 7 0.0601366061359343 1101 8 0.08791377195139205 1101 9 0.12552031241389017 1101 10-11 0.10200143630472525 1101 12-13 0.11207968093670928 1101 14-15 0.10731521704638425 1101 16-17 0.07444993020005342 1101 18-19 0.09567589692894529 1101 20-21 0.13461684863138146 1101 22-23 0.046905889412748536 1101 24-25 0.0969755590231216 1101 26-27 0.17193712952779805 1101 28-29 0.09359153698584066 1101 30-31 0.15353962451536773 1101 32-33 0.2915075652281871 1101 34-35 0.2869155288715248 1101 36-37 0.17151714548265318 1101 38-39 0.22166448854242304 1101 40-41 0.38540160639836074 1101 42-43 0.28301718708335954 1101 44-45 0.35638967961989465 1101 46-47 0.36685537226804854 1101 48-49 0.18605277195574388 1101 50-51 0.37053165695412815 1101 52-53 0.34674123633161 1101 54-55 0.2905358300745604 1101 56-57 0.22398120198329252 1101 58-59 0.293430369045673 1101 60-61 0.42737799824236333 1101 62-63 0.30991061727380753 1101 64-65 0.3203141412495114 1101 66-67 0.2654165937419535 1101 68-69 0.25363310485514745 1101 70-71 0.2888774412325006 1101 72-73 0.25107520715251397 1101 74-75 0.1942992871234388 1101 76-77 0.14337222205978506 1101 78-79 0.2844969120371843 1101 80-81 0.27672087167535864 1101 82-83 0.41755881205740053 1101 84-85 0.5377896104568229 1101 86-87 0.47397581146696055 1101 88-89 0.4796744669811055 1101 90-91 0.42746172852973174 1101 92-93 0.39684580138916203 1101 94-95 0.4615699625624323 1101 96-97 0.41480642783023924 1101 98-99 0.5225161237238538 1101 100-101 0.9354621689005711 1102 1 0.10630991851265037 1102 2 0.14008901121892592 1102 3 0.18423512686401722 1102 4 0.10755422804085413 1102 5 0.08720982869976268 1102 6 0.08690477274624442 1102 7 0.058720593489994144 1102 8 0.20184510670831912 1102 9 0.12317998128990126 1102 10-11 0.26105708121999527 1102 12-13 0.2523366902824975 1102 14-15 0.34053778284133074 1102 16-17 0.43294455012850364 1102 18-19 0.23695726912451676 1102 20-21 0.24552487436698556 1102 22-23 0.2385386301986827 1102 24-25 0.09092783168736673 1102 26-27 0.12692312470237255 1102 28-29 0.2431937167467879 1102 30-31 0.23979797887905363 1102 32-33 0.24094522295580134 1102 34-35 0.18447134018077094 1102 36-37 0.2971560289791455 1102 38-39 0.2652352337100652 1102 40-41 0.24470961549455694 1102 42-43 0.35108618511436873 1102 44-45 0.4591390625756091 1102 46-47 0.32650109178329245 1102 48-49 0.1997698051513339 1102 50-51 0.2802389422365863 1102 52-53 0.35058618488814375 1102 54-55 0.2869706313724336 1102 56-57 0.2107293785827551 1102 58-59 0.15227989343625126 1102 60-61 0.14001457995566824 1102 62-63 0.09566791780904538 1102 64-65 0.1993096070139515 1102 66-67 0.2531742270651094 1102 68-69 0.24193657971579086 1102 70-71 0.0647663316620779 1102 72-73 0.22704320404649891 1102 74-75 0.33749100070395954 1102 76-77 0.22859976580753028 1102 78-79 0.1605504631617336 1102 80-81 0.1855538953310365 1102 82-83 0.30640251265919005 1102 84-85 0.4235972451666008 1102 86-87 0.2837927087741505 1102 88-89 0.34152698646306234 1102 90-91 0.32521742058996495 1102 92-93 0.37449493198069206 1102 94-95 0.31004385257601896 1102 96-97 0.36212880227212807 1102 98-99 0.42369551028476593 1102 100-101 0.8053884702400431 1103 1 0.07012379705538763 1103 2 0.08379681451860677 1103 3 0.0634351893916687 1103 4 0.10534831625677299 1103 5 0.03484778709616165 1103 6 0.06926991909664793 1103 7 0.1166684748518847 1103 8 0.12655418544232333 1103 9 0.13760217925591434 1103 10-11 0.09325802486830526 1103 12-13 0.15884872090590108 1103 14-15 0.20726186118047707 1103 16-17 0.1464097531648818 1103 18-19 0.1183888008032099 1103 20-21 0.16125689415081723 1103 22-23 0.08321731594963211 1103 24-25 0.15636104663645511 1103 26-27 0.20500263867427293 1103 28-29 0.1885565285872346 1103 30-31 0.20678462197705016 1103 32-33 0.2482894501280697 1103 34-35 0.09683188350064853 1103 36-37 0.07377267442463165 1103 38-39 0.19392700212841163 1103 40-41 0.2509332400214106 1103 42-43 0.3269897800217123 1103 44-45 0.25838851437684696 1103 46-47 0.37391108928434846 1103 48-49 0.37804305478272227 1103 50-51 0.44026443469594057 1103 52-53 0.4805083444504987 1103 54-55 0.3811890816818817 1103 56-57 0.4064295070998014 1103 58-59 0.3039101289102035 1103 60-61 0.3091504623907042 1103 62-63 0.30376188183508646 1103 64-65 0.23122122504138787 1103 66-67 0.2408455019909823 1103 68-69 0.2172756137392753 1103 70-71 0.2700964490041926 1103 72-73 0.32475645525729036 1103 74-75 0.44933896330853074 1103 76-77 0.23052211252012356 1103 78-79 0.30466331692166904 1103 80-81 0.48513086005700146 1103 82-83 0.4885523010063295 1103 84-85 0.2880429315698656 1103 86-87 0.3409898931907023 1103 88-89 0.5412979708192509 1103 90-91 0.7016619527197676 1103 92-93 0.6110092965731653 1103 94-95 0.41865590094083416 1103 96-97 0.5677883256772667 1103 98-99 0.8721492084492901 1103 100-101 1.3061867660910416 1104 1 0.042102483483809294 1104 2 0.06034451921503603 1104 3 0.019673622001569413 1104 4 -0.010822827883799846 1104 5 -0.0013568668526957595 1104 6 -0.017367480258322132 1104 7 0.07446006986928921 1104 8 0.05096824773227837 1104 9 2.665423921115462E-4 1104 10-11 0.12008892562184315 1104 12-13 -0.019234106177684396 1104 14-15 -0.023515704024390516 1104 16-17 0.1554313764548496 1104 18-19 0.0655031100075476 1104 20-21 0.09293784496474444 1104 22-23 0.059666620535075765 1104 24-25 0.02086281676303514 1104 26-27 0.13562168829051302 1104 28-29 0.12277605439548722 1104 30-31 0.03175186226796711 1104 32-33 0.10528592654376467 1104 34-35 0.13295231932172413 1104 36-37 0.06116509299182127 1104 38-39 0.19987893179222738 1104 40-41 0.21534950152696553 1104 42-43 0.3215324050140538 1104 44-45 0.2591842513399101 1104 46-47 0.37263356817133797 1104 48-49 0.3485808920450637 1104 50-51 0.3342400626553328 1104 52-53 0.2772009446546093 1104 54-55 0.24159750129006596 1104 56-57 0.19408065612273617 1104 58-59 0.3111463010060831 1104 60-61 0.39037182285019867 1104 62-63 0.2429395230715059 1104 64-65 0.39046190956202764 1104 66-67 0.41747094546361296 1104 68-69 0.4826373228685483 1104 70-71 0.43784444148062107 1104 72-73 0.3930150797850551 1104 74-75 0.28414090485564003 1104 76-77 0.3247199876154312 1104 78-79 0.35248563643274267 1104 80-81 0.36591724363246314 1104 82-83 0.48093563503868353 1104 84-85 0.4250470057646254 1104 86-87 0.5472103798787273 1104 88-89 0.7074542205167909 1104 90-91 0.6651518148198079 1104 92-93 0.6403044345322719 1104 94-95 0.642853630855651 1104 96-97 0.6230572617838668 1104 98-99 0.7724625025731342 1104 100-101 1.0939094299487344 1105 1 0.01367542044484793 1105 2 -0.0015111092521706837 1105 3 0.02202938455298664 1105 4 0.02292802427786711 1105 5 0.07120732394104579 1105 6 0.019936603332681102 1105 7 -0.014470471281640584 1105 8 -0.029800927116752973 1105 9 0.05377340976506417 1105 10-11 0.07533367775992161 1105 12-13 0.004732637708563914 1105 14-15 0.010445563410591774 1105 16-17 0.009638227996170201 1105 18-19 -0.11549340218765991 1105 20-21 0.06993412453192605 1105 22-23 0.05999860010258118 1105 24-25 0.010647579304752242 1105 26-27 0.001247506123384312 1105 28-29 0.004246425017150557 1105 30-31 -0.06948474142758698 1105 32-33 -0.032467621841249183 1105 34-35 0.03575278102372437 1105 36-37 -0.0029950067797486213 1105 38-39 0.004360130925043393 1105 40-41 -0.09488419270289228 1105 42-43 -0.09385137467428706 1105 44-45 -0.012370390396789332 1105 46-47 0.0325590706640142 1105 48-49 -0.04115403317472044 1105 50-51 -0.03489900366626131 1105 52-53 -0.12782503214074836 1105 54-55 -0.11542834949844405 1105 56-57 -0.09016755958805334 1105 58-59 -0.11188209576685182 1105 60-61 -0.05893667421125315 1105 62-63 -0.18666081714696503 1105 64-65 -0.18502507457726125 1105 66-67 -0.11245588171637877 1105 68-69 -0.044879825370422566 1105 70-71 0.04930395612810656 1105 72-73 -0.10875507404022144 1105 74-75 -0.06823359717500921 1105 76-77 -0.10629308317133379 1105 78-79 -0.11430550237833614 1105 80-81 -0.19945769134477587 1105 82-83 -0.21671599319589774 1105 84-85 -0.19425906001094617 1105 86-87 -0.11478535042785865 1105 88-89 0.14277676572578812 1105 90-91 0.08995339442867234 1105 92-93 0.00749604893840683 1105 94-95 -0.2674834132176649 1105 96-97 -0.20999073874600782 1105 98-99 -0.14831271414248093 1105 100-101 0.283417865945335 1106 1 -0.05972102742245511 1106 2 -0.08870976108774897 1106 3 -0.07206996923376607 1106 4 -0.0059323538172861845 1106 5 -0.0683348410000093 1106 6 -0.13823736353552363 1106 7 -0.02110981836383985 1106 8 -0.021481174902504563 1106 9 -0.09176551597575155 1106 10-11 -0.06693864547951023 1106 12-13 -0.03021444193080214 1106 14-15 0.008814162316475915 1106 16-17 -0.10907655410187544 1106 18-19 -0.22328548425521433 1106 20-21 -0.1982815764418362 1106 22-23 -0.27105407863815145 1106 24-25 -0.19791633969550304 1106 26-27 -0.13707429159274653 1106 28-29 -0.13797076034558842 1106 30-31 -0.22332028414297156 1106 32-33 -0.19952538740112402 1106 34-35 -0.18663034334572615 1106 36-37 -0.26341079837431636 1106 38-39 -0.1689855527592954 1106 40-41 -0.12114215635909886 1106 42-43 -0.08110185744740761 1106 44-45 -0.019783801261844758 1106 46-47 -0.07740519039109728 1106 48-49 -0.012470469649088045 1106 50-51 0.09029517900105333 1106 52-53 0.019530843316722724 1106 54-55 0.027265075106413406 1106 56-57 0.010255581945472159 1106 58-59 -0.012312891264400605 1106 60-61 -0.019004517134845855 1106 62-63 -0.03260032256221024 1106 64-65 -0.053383693994170756 1106 66-67 -0.08283624105799703 1106 68-69 -0.12569541790858096 1106 70-71 -0.10086348433565462 1106 72-73 -0.02115665593057159 1106 74-75 0.06761359663375899 1106 76-77 -0.10559769253781681 1106 78-79 -0.09971743365480279 1106 80-81 -0.09357076626215033 1106 82-83 0.08135276766999766 1106 84-85 0.12796935827738665 1106 86-87 0.11159416991128523 1106 88-89 0.27027460488106314 1106 90-91 0.4594369065367374 1106 92-93 0.5092366590313446 1106 94-95 0.3594723812731324 1106 96-97 0.39707208323052257 1106 98-99 0.24904602821789013 1106 100-101 0.7387725871743633 1107 1 -0.1518051762252952 1107 2 -0.05803069691040008 1107 3 -0.1259226125420625 1107 4 -0.0642545482262733 1107 5 -0.05772246000315562 1107 6 -0.0575608186412353 1107 7 -0.05298402515239786 1107 8 -0.058469922016868736 1107 9 -0.150788756955599 1107 10-11 -0.12031506514828294 1107 12-13 -0.09889570408369508 1107 14-15 -0.19540373746669815 1107 16-17 -0.19287813114669916 1107 18-19 -0.1051858467417972 1107 20-21 -0.12914941537971458 1107 22-23 -0.1893090807340414 1107 24-25 -0.15387379784176147 1107 26-27 -0.19512213475669427 1107 28-29 -0.15565982066883777 1107 30-31 -0.1920735898776229 1107 32-33 -0.192046941780319 1107 34-35 -0.13179874093132327 1107 36-37 -0.09849767143225563 1107 38-39 -0.06493764522832635 1107 40-41 -0.11823950699660202 1107 42-43 -0.14341632412045158 1107 44-45 -0.20000579362696413 1107 46-47 -0.1324953160940936 1107 48-49 -0.27267007073182015 1107 50-51 -0.19811683114677692 1107 52-53 -0.045055660554140786 1107 54-55 -0.06661574460235187 1107 56-57 -0.03988111883835188 1107 58-59 -0.0965749299421148 1107 60-61 -0.18877682670540707 1107 62-63 -0.2262162614198715 1107 64-65 -0.2047155769590958 1107 66-67 -0.14349859882731408 1107 68-69 -0.07568778091960127 1107 70-71 -0.1582606665900883 1107 72-73 -0.12982190810553007 1107 74-75 -0.09224533406643332 1107 76-77 -0.21431741926286918 1107 78-79 -0.11078941893691763 1107 80-81 -0.020892153709155536 1107 82-83 -0.09586659540775955 1107 84-85 -0.05985757146046922 1107 86-87 -0.049893259657604716 1107 88-89 0.10100265658361351 1107 90-91 0.2512478384314143 1107 92-93 0.33199091602618225 1107 94-95 0.2321016501719093 1107 96-97 0.26034505137596753 1107 98-99 0.07286926582110453 1107 100-101 0.7462792593175074 1108 1 -0.13047350728187013 1108 2 -0.06319658633837832 1108 3 -0.16038922268228362 1108 4 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-3.5058700836266077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 18.0 4 34.0 5 105.0 6 225.0 7 547.0 8 1166.0 9 2167.0 10 3291.0 11 5029.0 12 7148.0 13 9751.0 14 11691.0 15 15460.0 16 19731.0 17 26359.0 18 24771.0 19 17309.0 20 15608.0 21 16510.0 22 17223.0 23 18838.0 24 22290.0 25 26688.0 26 32019.0 27 38999.0 28 47208.0 29 58022.0 30 70434.0 31 84660.0 32 103929.0 33 131160.0 34 174103.0 35 247757.0 36 374570.0 37 573432.0 38 468479.0 39 46873.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22997648307037 19.31577091839585 14.83479144216082 22.61946115637297 2 16.01138998500891 20.75167710048452 39.08259544842815 24.15433746607842 3 19.82166205043315 24.872642072632345 29.693080661494715 25.61261521543979 4 13.550389665140042 16.98654781892179 35.82862988518624 33.634432630751924 5 13.881019099266956 37.69138697794674 33.442990375926996 14.984603546859315 6 32.060220842183774 35.56414844863599 17.044441136020918 15.331189573159318 7 28.729273013238444 32.98515041944095 20.088981033397552 18.196595533923052 8 26.794398020055926 34.61979826150533 19.114007455745334 19.471796262693413 9 26.855202586073467 16.761497222152613 19.90413515270422 36.4791650390697 10-11 25.535946185379487 26.477993169251906 26.28470835182038 21.70135229354823 12-13 26.709805970787276 23.87069337841961 27.031038335620565 22.388462315172543 14-15 23.391060968711365 25.170207159848246 25.842338881303657 25.596392990136735 16-17 22.251304655781297 28.41496253433232 26.570552755411637 22.763180054474745 18-19 22.217226819221363 26.748006158594873 29.141021209416547 21.893745812767218 20-21 23.318348385841453 25.72847555214231 28.92878569228025 22.024390369735983 22-23 22.829958741338114 25.80333887084815 28.897167317951126 22.469535069862605 24-25 22.742547571281378 25.906909314964704 29.124926481751995 22.22561663200192 26-27 22.583448831427077 25.94813770148905 29.197647855678543 22.270765611405327 28-29 22.210046977976216 26.283068471100513 29.25930457265077 22.2475799782725 30-31 22.429643589429883 25.98418639068518 29.066185585853837 22.519984434031098 32-33 22.26023838337979 26.171888243418735 28.926371935265614 22.641501437935858 34-35 22.79079049492632 26.0858276694968 28.808781114861205 22.314600720715674 36-37 22.906553096130136 25.802528062451508 28.900021930269116 22.390896911149238 38-39 22.666892468257707 25.97368182946464 29.04616211313434 22.313263589143318 40-41 22.58882378825727 26.131198206239688 28.960815300032504 22.31916270547054 42-43 22.59960534314306 26.25931155614168 28.419679185139913 22.721403915575348 44-45 22.55305725387126 26.34374949904268 28.347468237693402 22.755725009392663 46-47 22.832953236163338 26.48361810985972 27.929228038217683 22.754200615759256 48-49 23.011570191895597 26.505807206707228 27.66623513834518 22.816387463052 50-51 22.79657178781995 26.771216549522205 27.69452841445739 22.737683248200454 52-53 22.970269883830113 26.861430447683755 27.423909534598167 22.744390133887972 54-55 22.62069890610743 26.661364999859966 27.61905534026235 23.098880753770253 56-57 22.759020080984314 26.90594676025902 27.304382498308527 23.030650660448142 58-59 22.920531455976224 26.540553846879533 27.436816452818064 23.10209824432618 60-61 22.74999414983462 26.4730393976854 27.889203211160385 22.887763241319597 62-63 22.728359839210615 26.546554923843203 27.804325010355203 22.920760226590982 64-65 22.566951354873137 26.651599418045024 27.714242124518908 23.067207102562932 66-67 22.57989326620414 26.735575800692335 27.426638710910172 23.257892222193355 68-69 22.5154813707628 26.64516742433623 27.578785104865005 23.260566100035966 70-71 22.565322459538816 26.623040049823892 27.385949993689096 23.425687496948193 72-73 22.589596449308154 26.585211887329507 27.377919171937627 23.447272491424716 74-75 22.406132490569764 27.0550285007584 27.10592007995248 23.43291892871936 76-77 22.52607982683596 26.663071378338742 27.313746020545153 23.497102774280137 78-79 22.495330165673593 26.708263551551397 27.360233987546373 23.43617229522864 80-81 22.46759851268237 26.81816841771662 27.223643043768263 23.49059002583275 82-83 22.448566707399582 26.760200058077572 27.2275107863615 23.563722448161347 84-85 22.281372576477406 26.656076845179044 27.16989385365336 23.89265672469019 86-87 22.431536804157727 26.569610246394433 27.163120331803192 23.83573261764465 88-89 22.394044512719834 26.449713603840674 27.098428402808782 24.05781348063071 90-91 22.234417492896256 26.51475424497047 27.222412542355467 24.02841571977781 92-93 22.262467884133766 26.56671255875932 27.16401607893833 24.00680347816858 94-95 22.096991407944728 26.34686284311516 27.219795464457608 24.336350284482506 96-97 22.05686445109487 26.155499074848976 27.274745717053772 24.512890757002385 98-99 22.084969303732105 26.19581305817154 27.034697589386212 24.684520048710137 100-101 22.14207023193775 25.7981249593482 27.498139460409433 24.561665348304622 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2153.0 1 1418.0 2 925.5 3 1210.0 4 1037.5 5 924.5 6 1563.5 7 3065.0 8 4482.5 9 5075.5 10 4554.0 11 3587.0 12 3329.5 13 3496.5 14 3734.0 15 3956.5 16 4087.5 17 4292.5 18 4595.5 19 4886.5 20 5334.5 21 6099.0 22 7002.0 23 8157.0 24 9554.0 25 11513.0 26 14358.5 27 17612.0 28 20406.0 29 23360.0 30 27081.5 31 31528.5 32 36467.5 33 40375.5 34 44293.5 35 48474.5 36 53817.5 37 59179.0 38 62508.0 39 65125.0 40 68206.5 41 72383.5 42 76747.0 43 81182.0 44 85883.5 45 90738.0 46 94469.0 47 99958.0 48 114970.5 49 116247.5 50 104550.0 51 100317.0 52 97646.0 53 98062.5 54 97324.0 55 92553.5 56 85516.0 57 77477.5 58 71614.0 59 65306.5 60 55682.5 61 46281.5 62 38514.5 63 30200.0 64 22811.0 65 17681.0 66 13687.5 67 10973.5 68 8325.0 69 5968.5 70 4464.0 71 3383.0 72 2681.0 73 2312.0 74 1847.0 75 1426.0 76 1014.0 77 609.5 78 389.5 79 259.0 80 168.5 81 97.5 82 52.5 83 32.5 84 21.0 85 12.0 86 7.0 87 5.5 88 3.5 89 2.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3852061385341751 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.5795876492291453E-4 16-17 0.002247926380042541 18-19 2.2110751279106963E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.1055375639553481E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007296547922105297 36-37 0.017209534745571586 38-39 0.011626570047597078 40-41 0.007591357939160056 42-43 0.0042563196212280895 44-45 0.0021189469975810837 46-47 0.001897839484790014 48-49 3.685125213184493E-5 50-51 4.053637734502943E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.6851252131844933E-4 60-61 4.974919037799067E-4 62-63 0.0 64-65 0.0 66-67 0.0014187732070760298 68-69 0.0010318350596916581 70-71 0.0020083932411855488 72-73 0.0 74-75 0.0 76-77 0.09789535128824607 78-79 0.03644588835839464 80-81 7.370250426368987E-5 82-83 0.0 84-85 0.0 86-87 0.14939497614249936 88-89 0.3272206933047171 90-91 0.28172782254795453 92-93 0.0 94-95 1.8425626065922467E-5 96-97 3.685125213184493E-5 98-99 3.1323564312068196E-4 100-101 6.448969123072864E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2713612.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.69057663324829 #Duplication Level Percentage of deduplicated Percentage of total 1 70.80640333288413 35.184110109373655 2 15.476353202106774 15.380578295850084 3 5.663423409878844 8.44256324865351 4 2.7504533384358325 5.466864495588773 5 1.4423682693191855 3.583605550998535 6 0.9023180077026177 2.690202126558411 7 0.5862530978539204 2.039187813977261 8 0.4184755234283344 1.663543205284347 9 0.28680678525510167 1.2826435087488794 >10 1.5128360687954088 13.328536688041712 >50 0.10485509932652116 3.5779499383466926 >100 0.04507753138019821 4.028725736701895 >500 0.0028928307066994736 0.987132507417531 >1k 0.0012609774875161068 1.2403105073078113 >5k 7.417514632447687E-5 0.25844870218544386 >10k+ 1.4835029264895374E-4 0.8455975649655525 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11996 0.4420676205736118 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10946 0.4033738058351746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7012 0.2584009799484967 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4330 0.15956592173088857 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3276 0.12072470198392402 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3122 0.11504960915561988 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3039 0.11199095522867676 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2990 0.11018524387421637 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.685125213184493E-5 2 0.0 0.0 0.0 0.0 3.685125213184493E-5 3 0.0 0.0 0.0 0.0 3.685125213184493E-5 4 0.0 0.0 0.0 0.0 3.685125213184493E-5 5 0.0 0.0 0.0 0.0 3.685125213184493E-5 6 0.0 0.0 0.0 0.0 3.685125213184493E-5 7 0.0 0.0 0.0 0.0 7.370250426368987E-5 8 0.0 0.0 0.0 0.0 1.1055375639553481E-4 9 0.0 0.0 0.0 3.685125213184493E-5 1.4740500852737973E-4 10-11 0.0 0.0 0.0 3.685125213184493E-5 1.4740500852737973E-4 12-13 0.0 0.0 0.0 3.685125213184493E-5 1.4740500852737973E-4 14-15 0.0 0.0 0.0 3.685125213184493E-5 2.5795876492291453E-4 16-17 0.0 0.0 0.0 3.685125213184493E-5 2.5795876492291453E-4 18-19 0.0 0.0 0.0 3.685125213184493E-5 2.5795876492291453E-4 20-21 0.0 0.0 0.0 7.370250426368987E-5 3.500868952525269E-4 22-23 0.0 0.0 0.0 3.500868952525269E-4 3.685125213184494E-4 24-25 0.0 0.0 0.0 7.554506687028212E-4 3.869381473843718E-4 26-27 0.0 0.0 0.0 0.0015661782156034098 4.053637734502943E-4 28-29 3.685125213184493E-5 0.0 0.0 0.0041089146127007104 4.790662777139842E-4 30-31 3.685125213184493E-5 0.0 0.0 0.01188452881251999 4.790662777139842E-4 32-33 3.685125213184493E-5 0.0 0.0 0.02719622407330156 4.790662777139842E-4 34-35 3.685125213184493E-5 0.0 0.0 0.05098370732440747 4.790662777139842E-4 36-37 3.685125213184493E-5 0.0 0.0 0.09081991087893185 4.790662777139842E-4 38-39 3.685125213184493E-5 0.0 0.0 0.14760769041410488 5.159175298458292E-4 40-41 3.685125213184493E-5 0.0 0.0 0.20959149649986808 5.52768781977674E-4 42-43 3.685125213184493E-5 0.0 0.0 0.2716858563420268 5.52768781977674E-4 44-45 3.685125213184493E-5 0.0 0.0 0.3522979703804376 5.52768781977674E-4 46-47 3.685125213184493E-5 0.0 0.0 0.4533441037259564 5.896200341095189E-4 48-49 3.685125213184493E-5 0.0 0.0 0.5596231148741972 7.370250426368987E-4 50-51 3.685125213184493E-5 0.0 0.0 0.672774884545027 8.844300511642784E-4 52-53 3.685125213184493E-5 0.0 0.0 0.7869584892755486 9.212813032961234E-4 54-55 3.685125213184493E-5 0.0 0.0 0.9122711721498873 9.581325554279684E-4 56-57 3.685125213184493E-5 0.0 0.0 1.07181866825471 9.949838075598134E-4 58-59 3.685125213184493E-5 0.0 0.0 1.2483730172183791 9.949838075598134E-4 60-61 3.685125213184493E-5 0.0 0.0 1.4408655327290711 9.949838075598134E-4 62-63 3.685125213184493E-5 0.0 0.0 1.6359192102629263 0.0010134094336257359 64-65 3.685125213184493E-5 0.0 0.0 1.8343816286189774 0.0010318350596916581 66-67 3.685125213184493E-5 0.0 0.0 2.070100662880323 0.0010318350596916581 68-69 3.685125213184493E-5 0.0 0.0 2.336885302688815 0.0010318350596916581 70-71 5.52768781977674E-5 0.0 0.0 2.6346618455401876 0.0010318350596916581 72-73 7.370250426368987E-5 0.0 0.0 2.9341703972417577 0.0010502606857575806 74-75 7.370250426368987E-5 0.0 0.0 3.2366270491138747 0.0010686863118235031 76-77 7.370250426368987E-5 0.0 0.0 3.5672196319886558 0.0010686863118235031 78-79 7.370250426368987E-5 0.0 0.0 3.943010275603144 0.0010686863118235031 80-81 1.1055375639553481E-4 0.0 0.0 4.355467915088818 0.0010686863118235031 82-83 1.4740500852737973E-4 0.0 0.0 4.776769855086136 0.0010686863118235031 84-85 1.4740500852737973E-4 0.0 0.0 5.20089091587154 0.0010686863118235031 86-87 1.4740500852737973E-4 0.0 0.0 5.639144431849505 0.0010686863118235031 88-89 1.4740500852737973E-4 0.0 0.0 6.113549026168811 0.0010686863118235031 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2235 0.0 24.84047 2 GTACATG 15735 0.0 23.940905 1 GGTATCA 16950 0.0 23.713932 1 TACATGG 15925 0.0 23.509808 2 ACATGGG 15865 0.0 22.70143 3 GTATCAA 22205 0.0 22.455706 1 GAGTACT 11915 0.0 22.163921 12-13 GTACTTT 12635 0.0 20.731913 14-15 GTACCTG 2835 0.0 20.6625 1 AGTACTT 12185 0.0 19.920286 12-13 CATGGGG 6390 0.0 19.827223 4 CATGGGA 9495 0.0 19.540394 4 TATCAAC 26270 0.0 18.93009 2 ATCAACG 26240 0.0 18.879398 3 TCAACGC 26290 0.0 18.861542 4 CAACGCA 26480 0.0 18.708288 5 ACTTTTT 14485 0.0 18.378407 16-17 AACGCAG 27055 0.0 18.328218 6 ATGGGAG 3440 0.0 18.208199 5 TACTTTT 13990 0.0 17.265358 14-15 >>END_MODULE