FastQCFastQC Report
Thu 26 May 2016
SRR938692_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938692_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719014
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129180.4750986938647612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104730.3851763911476734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66390.24416939375817853No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42700.15704222192309417No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31090.11434291989669784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29330.10786998522258437No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29290.10772287307089995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27890.10257394776194606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG151900.025.3403431
GTATCAA210700.025.2152041
TACATGG153650.024.764512
ACATGGG154450.024.021873
GGTATCA155450.023.5999891
GAGTACT112500.022.61520212-13
ATCAACG246750.021.3813723
TATCAAC247450.021.3592342
CATGGGA90000.021.2974114
TCAACGC248500.021.2498914
GTACTTT119650.021.10566914-15
CAACGCA251400.021.0047655
AACGCAG255650.020.765016
AGTACTT115550.020.74481812-13
CATGGGG66800.019.318814
ACGCAGA276500.019.1491287
ATGGGAT35000.018.977895
CGCAGAG279150.018.9160068
ACTTTTT137900.018.55482116-17
TACTTTT132700.018.20736314-15
Produced by FastQC (version 0.11.5)