##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938692_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2719014 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58295838123673 33.0 31.0 34.0 30.0 34.0 2 31.65688076633662 33.0 31.0 34.0 30.0 34.0 3 31.669220901400287 33.0 31.0 34.0 28.0 34.0 4 35.21149615264945 37.0 35.0 37.0 33.0 37.0 5 35.24091895076671 37.0 35.0 37.0 33.0 37.0 6 35.29626254223038 37.0 35.0 37.0 33.0 37.0 7 35.20858002202269 37.0 35.0 37.0 32.0 37.0 8 35.174098772569764 37.0 35.0 37.0 32.0 37.0 9 36.750845710982 39.0 37.0 39.0 33.0 39.0 10-11 36.63040627962931 39.0 37.0 39.0 32.0 39.0 12-13 36.51437855781545 39.0 37.0 39.0 32.0 39.0 14-15 37.678361531055 40.0 38.0 41.0 32.0 41.0 16-17 37.561969890555915 40.0 37.0 41.0 32.0 41.0 18-19 37.51107147664558 40.0 37.0 41.0 32.0 41.0 20-21 37.40237987005584 40.0 37.0 41.0 32.0 41.0 22-23 37.41229155127557 40.0 37.0 41.0 32.0 41.0 24-25 37.34257804483537 40.0 37.0 41.0 31.0 41.0 26-27 37.19979466821428 40.0 37.0 41.0 31.0 41.0 28-29 37.01928088638013 40.0 37.0 41.0 30.5 41.0 30-31 36.78292940014285 39.5 36.0 41.0 30.0 41.0 32-33 36.58026420606882 39.0 35.5 41.0 30.0 41.0 34-35 36.28301417352026 39.0 35.0 41.0 30.0 41.0 36-37 36.06766717641027 39.0 35.0 41.0 29.0 41.0 38-39 35.88199012583238 39.0 35.0 41.0 28.5 41.0 40-41 35.7499558663545 39.0 35.0 41.0 27.0 41.0 42-43 35.7265999733727 39.0 35.0 41.0 27.0 41.0 44-45 35.51786346079866 39.0 35.0 41.0 26.5 41.0 46-47 35.17777345022865 39.0 35.0 41.0 24.5 41.0 48-49 34.859347726786254 39.0 35.0 41.0 24.0 41.0 50-51 33.70000080911683 38.0 33.0 40.0 22.0 40.5 52-53 33.754403066699915 38.0 33.5 39.5 22.0 40.5 54-55 34.22093431663096 38.0 34.0 40.0 22.0 41.0 56-57 34.098083165441594 38.0 34.0 40.0 21.5 41.0 58-59 33.87433220277644 38.0 34.0 40.0 20.5 41.0 60-61 33.54699479296539 37.5 33.0 40.0 19.5 41.0 62-63 33.13768318221238 37.0 33.0 40.0 18.0 41.0 64-65 32.730154938518154 36.0 32.0 39.5 14.5 41.0 66-67 32.33371490547677 36.0 32.0 39.0 12.0 41.0 68-69 31.910208443207722 35.0 31.5 39.0 8.0 40.0 70-71 31.480671118280377 35.0 31.0 38.0 7.0 40.0 72-73 31.05749161276845 35.0 31.0 37.0 4.0 39.5 74-75 30.59379503011018 35.0 30.5 37.0 2.0 39.0 76-77 30.141385811180083 35.0 30.0 36.0 2.0 39.0 78-79 29.720920524866735 34.0 29.5 36.0 2.0 38.5 80-81 29.271518278317068 34.0 29.0 35.0 2.0 37.0 82-83 28.87425625612814 34.0 29.0 35.0 2.0 37.0 84-85 28.533119910379277 34.0 29.0 35.0 2.0 36.0 86-87 28.118480081382444 34.0 27.5 35.0 2.0 36.0 88-89 27.676171398896805 34.0 27.0 35.0 2.0 35.5 90-91 27.24046382254744 34.0 26.0 35.0 2.0 35.0 92-93 26.789920905151646 33.0 25.0 35.0 2.0 35.0 94-95 26.281681337425994 33.0 24.5 35.0 2.0 35.0 96-97 25.683448669260255 33.0 21.5 35.0 2.0 35.0 98-99 24.93348287283552 32.5 10.0 35.0 2.0 35.0 100-101 22.503917044928784 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.009672403396454143 1101 2 0.10937595263954591 1101 3 0.05665897866257197 1101 4 -0.03310862749561494 1101 5 -0.11156170932871845 1101 6 0.01968505357065453 1101 7 0.09849331924087323 1101 8 0.13001446952536355 1101 9 0.23063200846703324 1101 10-11 0.10269173389794872 1101 12-13 0.1434369968507454 1101 14-15 0.15006685949501275 1101 16-17 0.2639584725631181 1101 18-19 0.3244360399123778 1101 20-21 0.3123413251232918 1101 22-23 0.3010840432814774 1101 24-25 0.2514539087822243 1101 26-27 0.2260518478569935 1101 28-29 0.26724407152052976 1101 30-31 0.2834645647747891 1101 32-33 0.2709729720588001 1101 34-35 0.17510550047787632 1101 36-37 0.3075693546540421 1101 38-39 0.23812137289467472 1101 40-41 0.32062875793394596 1101 42-43 0.47034957019367596 1101 44-45 0.5966462144300522 1101 46-47 0.49208465824304426 1101 48-49 0.4388656960392936 1101 50-51 0.530402384067834 1101 52-53 0.47406569265955056 1101 54-55 0.5389659864815997 1101 56-57 0.5695719204080376 1101 58-59 0.43351484876423285 1101 60-61 0.537389007803462 1101 62-63 0.4894999749741302 1101 64-65 0.44179501132592236 1101 66-67 0.502755116963634 1101 68-69 0.44725784715257433 1101 70-71 0.3985302395503112 1101 72-73 0.3001578008409673 1101 74-75 0.24915465261281255 1101 76-77 0.1339157766789505 1101 78-79 0.18836886804392705 1101 80-81 0.0975008463053193 1101 82-83 0.10942156892336996 1101 84-85 0.14040442556864363 1101 86-87 0.1017526108770852 1101 88-89 0.09185670599051576 1101 90-91 -0.03839999835561869 1101 92-93 0.01225834655656044 1101 94-95 -0.14662084317616575 1101 96-97 0.07945344826479683 1101 98-99 -0.04470575436251778 1101 100-101 0.22258544696703453 1102 1 0.15171649338855886 1102 2 0.0898333019362596 1102 3 0.12509262580226377 1102 4 0.29019892427209726 1102 5 0.39046558484729843 1102 6 0.30284546176873306 1102 7 0.19671359011528722 1102 8 0.3248548830985456 1102 9 0.43718007820905314 1102 10-11 0.45998650428007437 1102 12-13 0.3590905763364347 1102 14-15 0.463211064844792 1102 16-17 0.5557558775232039 1102 18-19 0.5180762281445155 1102 20-21 0.48522351982106926 1102 22-23 0.45307192861596235 1102 24-25 0.340056871810269 1102 26-27 0.44351888009988727 1102 28-29 0.5062546899421605 1102 30-31 0.5340629331195359 1102 32-33 0.4989933044368442 1102 34-35 0.5654070491807417 1102 36-37 0.4184623460823005 1102 38-39 0.4186659384800322 1102 40-41 0.547715811418847 1102 42-43 0.6180496443598216 1102 44-45 0.5947405062208659 1102 46-47 0.6616739535095846 1102 48-49 0.5987673935912952 1102 50-51 0.6172683862540609 1102 52-53 0.5056811450601231 1102 54-55 0.4272081970979542 1102 56-57 0.6225260330113827 1102 58-59 0.3601421755010037 1102 60-61 0.39273245908767507 1102 62-63 0.23855015728847206 1102 64-65 0.291347817881487 1102 66-67 0.2693214310611367 1102 68-69 0.2744393526478497 1102 70-71 0.30507688798984844 1102 72-73 0.21232272878092928 1102 74-75 0.11223722511861922 1102 76-77 0.11173596382572626 1102 78-79 0.04031476035054382 1102 80-81 -0.04281297728261535 1102 82-83 -0.007115931496560535 1102 84-85 -0.039158149211246496 1102 86-87 -0.11712693761387882 1102 88-89 -0.025418579626677484 1102 90-91 -0.04087254783632588 1102 92-93 -0.21566314069435322 1102 94-95 -0.13187379625509266 1102 96-97 -0.20306763223519297 1102 98-99 -0.23176246949990187 1102 100-101 0.013876222197094279 1103 1 0.09116244229128156 1103 2 0.19751197203589754 1103 3 0.301429272330747 1103 4 0.17035764971536338 1103 5 0.21209798422245996 1103 6 0.21552515612847856 1103 7 0.20339569258418067 1103 8 0.21362423909597084 1103 9 0.31219244553636827 1103 10-11 0.29748124412962085 1103 12-13 0.3396964113383305 1103 14-15 0.30765023658131696 1103 16-17 0.41323944738356033 1103 18-19 0.4260770041539459 1103 20-21 0.3544821414950263 1103 22-23 0.3865323494228292 1103 24-25 0.4758848705093399 1103 26-27 0.38811131628124684 1103 28-29 0.44180192770767945 1103 30-31 0.6639856137516205 1103 32-33 0.5671219075914635 1103 34-35 0.6179134898781484 1103 36-37 0.6569368365807051 1103 38-39 0.7184073935287074 1103 40-41 0.7341675109027435 1103 42-43 0.804452391185194 1103 44-45 0.8142147133363338 1103 46-47 0.7574496361537655 1103 48-49 0.7642711655292587 1103 50-51 0.8202827463033131 1103 52-53 0.6941176229796326 1103 54-55 0.7967211401033651 1103 56-57 0.825194947453241 1103 58-59 0.8107026382340834 1103 60-61 0.6591535573476719 1103 62-63 0.6116106180841072 1103 64-65 0.49965093790952153 1103 66-67 0.4414623568527887 1103 68-69 0.37711387275033914 1103 70-71 0.40434028609999473 1103 72-73 0.5815106338835534 1103 74-75 0.45689332558859164 1103 76-77 0.4338831410914388 1103 78-79 0.40474437663145224 1103 80-81 0.5410675968300289 1103 82-83 0.5605281824176949 1103 84-85 0.540016511549414 1103 86-87 0.3665554880200581 1103 88-89 0.4643656205217539 1103 90-91 0.3146478137572011 1103 92-93 0.3795247871409053 1103 94-95 0.19851182928109523 1103 96-97 0.32475652929876375 1103 98-99 0.48529038929660473 1103 100-101 0.7167341662319338 1104 1 -0.02922007048314157 1104 2 0.1008809890111948 1104 3 0.03391503867128165 1104 4 0.023194631909085217 1104 5 0.2076932323452283 1104 6 0.27433657891227625 1104 7 0.2265815594612448 1104 8 0.23202927586534372 1104 9 0.2285360326684156 1104 10-11 0.17937819045167203 1104 12-13 0.2066828217772354 1104 14-15 0.2875519462174978 1104 16-17 0.3317823837972327 1104 18-19 0.34556651144252015 1104 20-21 0.44303742409334035 1104 22-23 0.3540362924485194 1104 24-25 0.3211335084493001 1104 26-27 0.3868788229160671 1104 28-29 0.22384443691193212 1104 30-31 0.27973953082533853 1104 32-33 0.3493521339261605 1104 34-35 0.4035744641113794 1104 36-37 0.48444778341947625 1104 38-39 0.5867730579983998 1104 40-41 0.5429015285439007 1104 42-43 0.5397875940909884 1104 44-45 0.5865583968062253 1104 46-47 0.5325712111693903 1104 48-49 0.5974502037529703 1104 50-51 0.7391002713427852 1104 52-53 0.7727611835028654 1104 54-55 0.7779613404963968 1104 56-57 0.7844559511545128 1104 58-59 0.7982527744851637 1104 60-61 0.7078408768913036 1104 62-63 0.8136594845559912 1104 64-65 0.809996553803316 1104 66-67 0.6378544658041889 1104 68-69 0.6819807243135507 1104 70-71 0.6912456027385474 1104 72-73 0.6656533081758518 1104 74-75 0.5611116869127066 1104 76-77 0.588486132561929 1104 78-79 0.444953128384018 1104 80-81 0.42474956241062856 1104 82-83 0.4077890320814497 1104 84-85 0.4376574191755829 1104 86-87 0.4151361662024442 1104 88-89 0.5517462382225204 1104 90-91 0.4840680943167115 1104 92-93 0.5191283749272237 1104 94-95 0.6917461561564409 1104 96-97 0.4582952760463961 1104 98-99 0.6158288818050472 1104 100-101 1.13466797182679 1105 1 0.05551838999199532 1105 2 0.132790505912741 1105 3 0.20478317461344275 1105 4 0.1051601912702651 1105 5 0.18127539369109513 1105 6 0.09631162648674518 1105 7 0.07560068301359024 1105 8 0.18755245020376776 1105 9 0.2171591973763256 1105 10-11 0.23448510564142566 1105 12-13 0.17709974667626938 1105 14-15 0.2368870725029737 1105 16-17 0.27736027671762997 1105 18-19 0.15773520852076217 1105 20-21 0.2714427435912725 1105 22-23 0.2271477657413854 1105 24-25 0.18293472148712908 1105 26-27 0.24193105509828428 1105 28-29 0.3465580481274344 1105 30-31 0.426408403107736 1105 32-33 0.4854689898869964 1105 34-35 0.351081508487205 1105 36-37 0.35235198346577334 1105 38-39 0.32321569966538277 1105 40-41 0.3169689126455282 1105 42-43 0.21871473990989188 1105 44-45 0.38604105540664335 1105 46-47 0.5185096485719569 1105 48-49 0.5943458250213141 1105 50-51 0.6813745686267509 1105 52-53 0.601617155016875 1105 54-55 0.48693383865351336 1105 56-57 0.5109834226320658 1105 58-59 0.5505697509417402 1105 60-61 0.5768952750491536 1105 62-63 0.5248773655945271 1105 64-65 0.5272105079310805 1105 66-67 0.49835949844501926 1105 68-69 0.42273340084602395 1105 70-71 0.4789453056156816 1105 72-73 0.5741055808621205 1105 74-75 0.5411128761156583 1105 76-77 0.3523825280827033 1105 78-79 0.44610643925970805 1105 80-81 0.5219633462892581 1105 82-83 0.3919564972674081 1105 84-85 0.48738859070057927 1105 86-87 0.4600588159194139 1105 88-89 0.530862004842966 1105 90-91 0.5798000754144859 1105 92-93 0.6799753161218973 1105 94-95 0.7662103351020093 1105 96-97 0.7158573871255776 1105 98-99 0.762931571766881 1105 100-101 1.0519403569842183 1106 1 0.03480691465797392 1106 2 0.0237612122626345 1106 3 0.07269743967051667 1106 4 0.12102506563639537 1106 5 0.08706634920042688 1106 6 0.050719696315390195 1106 7 0.10704071147368666 1106 8 0.03741675897855856 1106 9 0.027239459660350462 1106 10-11 0.029065086057180167 1106 12-13 0.0843877757388114 1106 14-15 0.2651504412139971 1106 16-17 0.15634345130247596 1106 18-19 0.13045729679183893 1106 20-21 0.1554014279718885 1106 22-23 0.22231396291612526 1106 24-25 0.142528326669499 1106 26-27 -0.02115906447593119 1106 28-29 0.04434734519533379 1106 30-31 -0.09941243699201863 1106 32-33 0.04590466339266186 1106 34-35 0.18197674412821385 1106 36-37 0.32896736564612894 1106 38-39 0.16782244489245102 1106 40-41 0.3141443563855759 1106 42-43 0.4867031476367032 1106 44-45 0.5008392818684797 1106 46-47 0.4080528983183811 1106 48-49 0.3225553797065359 1106 50-51 0.2897544956489 1106 52-53 0.1537339739896808 1106 54-55 0.027367808213220712 1106 56-57 0.18198020998913478 1106 58-59 0.3070552251735137 1106 60-61 0.2696372814343775 1106 62-63 0.1368346517007737 1106 64-65 0.2652567026723176 1106 66-67 0.24665787100352787 1106 68-69 0.31553992965464417 1106 70-71 0.30695700225246725 1106 72-73 0.3527900826576307 1106 74-75 0.22021441188040924 1106 76-77 0.3280258078645666 1106 78-79 0.3413185860943422 1106 80-81 0.4278092039756416 1106 82-83 0.37888681144264424 1106 84-85 0.48477220519529496 1106 86-87 0.5073782480380018 1106 88-89 0.5460838807390687 1106 90-91 0.498143288460529 1106 92-93 0.5620285610940527 1106 94-95 0.7849620871182736 1106 96-97 0.769310097246656 1106 98-99 0.7210133267965837 1106 100-101 0.9543273775160266 1107 1 0.14975555268707552 1107 2 0.1715193709064664 1107 3 0.2568123105416049 1107 4 0.14253581403462334 1107 5 0.17417842592109167 1107 6 -0.03215499202540428 1107 7 -0.01619186494021818 1107 8 0.14485500311021582 1107 9 0.1312150731123083 1107 10-11 0.14664603191913272 1107 12-13 0.16060077320307897 1107 14-15 0.20026211805971883 1107 16-17 0.2776347052391941 1107 18-19 0.2169199406955613 1107 20-21 0.12436186352498879 1107 22-23 0.10925199212713466 1107 24-25 0.05743788711400555 1107 26-27 0.10370158286773545 1107 28-29 0.17977750253568558 1107 30-31 0.131496658549338 1107 32-33 0.09911447872104873 1107 34-35 0.22468541400171915 1107 36-37 0.2901219899826728 1107 38-39 0.01777501433765849 1107 40-41 0.11897917928137502 1107 42-43 0.15906744584101773 1107 44-45 0.37120257965750625 1107 46-47 0.41938151557000225 1107 48-49 0.3892553935492984 1107 50-51 0.271945866138978 1107 52-53 0.1695049519253473 1107 54-55 0.20479775518247578 1107 56-57 0.29200326467216087 1107 58-59 0.3390073076351001 1107 60-61 0.3542209013386852 1107 62-63 0.21837844558951502 1107 64-65 0.20398357170854808 1107 66-67 -0.04713246908064406 1107 68-69 0.16684826441452216 1107 70-71 0.2592589946997279 1107 72-73 0.17597656911690152 1107 74-75 0.14223157956958943 1107 76-77 0.05165580853062224 1107 78-79 0.12064888534420248 1107 80-81 0.14360015139414983 1107 82-83 -0.06992610228567386 1107 84-85 0.1593472804417857 1107 86-87 0.1964071160728409 1107 88-89 0.29834109350153426 1107 90-91 0.5317992069918311 1107 92-93 0.5952704100934589 1107 94-95 0.5221619337196479 1107 96-97 0.5764286112744905 1107 98-99 0.7964036446546991 1107 100-101 1.2661088143166772 1108 1 0.2741334450066688 1108 2 0.27142636685415766 1108 3 0.22775292865240004 1108 4 0.34036254214349526 1108 5 0.27378794813302676 1108 6 0.3070208932702272 1108 7 0.22298518508155496 1108 8 0.21513899681426807 1108 9 0.3499730011153659 1108 10-11 0.46043838427369366 1108 12-13 0.41675671905642986 1108 14-15 0.6543483455478807 1108 16-17 0.7076646520888659 1108 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-1.3501045419415831 2316 78-79 -1.24401315965833 2316 80-81 -1.210103408571296 2316 82-83 -1.2242896125080982 2316 84-85 -1.2184902325605371 2316 86-87 -1.2253253060557583 2316 88-89 -1.3567230209486176 2316 90-91 -1.6112253909363226 2316 92-93 -1.4839642866930767 2316 94-95 -1.2618661225510053 2316 96-97 -1.5703112013962297 2316 98-99 -1.7465507970251828 2316 100-101 -1.785552153128048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15733.0 3 3995.0 4 3660.0 5 4011.0 6 4727.0 7 5472.0 8 6779.0 9 7645.0 10 8888.0 11 10625.0 12 13330.0 13 15955.0 14 17269.0 15 19496.0 16 23176.0 17 28914.0 18 26095.0 19 19088.0 20 18992.0 21 20483.0 22 22093.0 23 24310.0 24 27756.0 25 31451.0 26 36130.0 27 41554.0 28 48509.0 29 57159.0 30 68117.0 31 82437.0 32 101508.0 33 129695.0 34 173150.0 35 244643.0 36 356518.0 37 505410.0 38 427306.0 39 66901.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.855523903797675 18.316237941885014 14.190072330249622 22.638165824067695 2 15.602860655233428 20.12928428728417 40.22279749035083 24.04505756713157 3 19.733648367351677 24.826063709636113 29.4085631817748 26.03172474123741 4 13.068111909029891 16.266270580668262 36.487875392052196 34.17774211824965 5 13.960298913854901 37.42627517813952 33.341738488347616 15.271687419657962 6 31.906340341716245 35.17462610390783 17.498539792272783 15.42049376210314 7 28.854996165870343 32.93683271397393 20.231190644723647 17.97698047543208 8 26.724607373853974 34.24884959811054 19.43201245578958 19.5945305722459 9 27.280539331404974 16.759186376600095 20.05184688887358 35.908427403121344 10-11 25.582077577420698 26.277181939816955 26.451409492609933 21.68933099015241 12-13 26.901106928517077 23.737698465131114 27.04729808049709 22.313896525854716 14-15 23.620506396735777 24.92293522211272 25.987657846017814 25.46890053513369 16-17 22.38654306703201 28.25932686565898 26.596020915991176 22.758109151317836 18-19 22.34579588430217 26.60260841003772 29.182485852588226 21.86910985307188 20-21 23.508834448341712 25.590050346737264 28.93258799623523 21.96852720868579 22-23 23.007189971535432 25.648287379123293 28.882677722886264 22.46184492645501 24-25 22.857169715715962 25.75890149067977 29.145035531127363 22.238893262476907 26-27 22.667627926150324 25.82312148903941 29.198552058308863 22.310698526501405 28-29 22.333502864598753 26.189112403287396 29.233738972397028 22.243645759716827 30-31 22.49937018811734 25.92119439627874 29.08285004723128 22.49658536837264 32-33 22.29864404793534 26.11192528095001 28.93102468586699 22.658405985247658 34-35 22.8783314114953 25.96227242460169 28.79340930130237 22.365986862600636 36-37 22.978702428287615 25.72001315232456 28.910522653512867 22.390761765874963 38-39 22.8164954284168 25.83818511451016 28.96857611066749 22.376743346405547 40-41 22.676941000507604 26.031254399549486 28.95690060630059 22.33490399364232 42-43 22.623181025427392 26.164310767856957 28.44497019926284 22.767538007452814 44-45 22.604207353676284 26.280897822781967 28.3584549851847 22.75643983835705 46-47 22.871980155963318 26.35531968271251 27.9591282550277 22.813571906296477 48-49 23.016466644349986 26.40198567978199 27.750144526040444 22.831403149827576 50-51 22.878797026018734 26.731325336572358 27.699174532068373 22.690703105340535 52-53 23.011103590804748 26.751323653923624 27.541011209195105 22.696561546076524 54-55 22.63452030233538 26.537347192033035 27.737934405879816 23.09019809975177 56-57 22.839660779884376 26.77902390144477 27.397061290814968 22.98425402785589 58-59 23.041630099504506 26.38194348592758 27.49366517740825 23.082761237159666 60-61 22.78535325585685 26.380852241283897 27.968917173953145 22.864877328906104 62-63 22.84299959076453 26.43781106833346 27.91296002764416 22.806229313257845 64-65 22.669402250042562 26.500560793361032 27.882066097908577 22.947970858687828 66-67 22.632707031204554 26.61050635685981 27.575802845661695 23.180983766273936 68-69 22.565912535454643 26.57467188006981 27.71302472365276 23.146390860822784 70-71 22.6999363253053 26.487491098839083 27.52089372087732 23.291678854978297 72-73 22.743201676069877 26.394075109232233 27.505675348987335 23.357047865710552 74-75 22.558013503170834 26.9131323091013 27.314423724072224 23.214430463655642 76-77 22.739556383290214 26.571199436996025 27.367742249926064 23.3215019297877 78-79 22.664372378921442 26.548616543013097 27.466190139927438 23.320820938138024 80-81 22.706780018931422 26.74019206091134 27.27084782355087 23.28218009660637 82-83 22.763192046872234 26.60683950970087 27.3243297228065 23.305638720620394 84-85 22.653063485001486 26.53916376062191 27.23755600558994 23.57021674878666 86-87 22.802133446160166 26.478789311946443 27.26660194629982 23.45247529559357 88-89 22.785854487774042 26.332663303223825 27.169191710334317 23.71229049866782 90-91 22.682358094116804 26.373116849591344 27.314318129023846 23.63020692726801 92-93 22.795904703062167 26.406433440714917 27.265843091910043 23.531818764312877 94-95 22.660804809347752 26.23243321802043 27.30203073947932 23.804731233152502 96-97 22.669834101185053 25.97580694604798 27.37352606817814 23.98083288458882 98-99 22.72531445829521 26.045151991638456 27.096310152873404 24.133223397192936 100-101 23.076954319443953 25.522104772276606 27.426924347215575 23.97401656106387 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3859.0 1 2543.0 2 1424.0 3 1604.5 4 1375.0 5 1307.0 6 1952.5 7 3447.0 8 5257.5 9 5816.5 10 4855.0 11 3941.5 12 3741.0 13 3851.5 14 4125.0 15 4353.5 16 4439.5 17 4532.5 18 4819.5 19 5144.0 20 5508.5 21 6264.5 22 7166.5 23 8300.5 24 9608.0 25 11625.0 26 14384.0 27 17518.0 28 20465.5 29 23471.5 30 27070.5 31 31291.0 32 36306.0 33 40308.5 34 44027.0 35 48273.5 36 53782.0 37 58996.5 38 62252.0 39 64742.5 40 67557.0 41 72092.0 42 76417.0 43 80837.0 44 85963.5 45 90655.5 46 93848.5 47 99044.5 48 113607.0 49 114897.5 50 103597.0 51 99335.0 52 97160.5 53 97406.0 54 96145.5 55 91758.0 56 85385.5 57 77454.5 58 71427.5 59 65157.0 60 55975.5 61 46942.0 62 39200.0 63 31078.5 64 23473.5 65 18283.0 66 14336.0 67 11422.5 68 8802.5 69 6405.5 70 4865.0 71 3740.0 72 2898.0 73 2433.5 74 1952.5 75 1527.5 76 1179.0 77 771.5 78 518.5 79 392.5 80 302.5 81 238.0 82 173.5 83 134.5 84 106.0 85 71.0 86 55.0 87 45.0 88 27.0 89 17.0 90 19.0 91 16.5 92 9.5 93 6.5 94 5.0 95 6.5 96 8.5 97 5.0 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2781155227593532 2 0.1666412898205011 3 0.19245947244111286 4 0.16373582482473426 5 0.2351219964295881 6 0.19709350521917138 7 0.2403076997764631 8 0.24644963210928666 9 0.150679621362744 10-11 0.20626962568048565 12-13 0.21277933839252022 14-15 0.17429112170808977 16-17 0.28639058129160055 18-19 0.2326946459267955 20-21 0.19977830198741162 22-23 0.2462105748627995 24-25 0.23510360741062752 26-27 0.34477571649134575 28-29 0.3162727371024938 30-31 0.2900683850837105 32-33 0.19400415003379903 34-35 0.3604983277026157 36-37 0.39527196255701513 38-39 0.37568765736402976 40-41 0.33907512061357536 42-43 0.37162368417374825 44-45 0.379843575649114 46-47 0.38589356288713483 48-49 0.4054594790611597 50-51 0.3969269742634647 52-53 0.39687180720658294 54-55 0.3909873211392071 56-57 0.3690859995571924 58-59 0.3452722200032806 60-61 0.3131282148602398 62-63 0.3789792917579682 64-65 0.4134954803469199 66-67 0.41737556334759585 68-69 0.4231497153012085 70-71 0.4230209921684846 72-73 0.41378970465028864 74-75 0.4093947291187173 76-77 0.39297333518694644 78-79 0.3665667039595971 80-81 0.3784460102081122 82-83 0.38782440987799255 84-85 0.38907486316730994 86-87 0.3839075488393955 88-89 0.3927158889214987 90-91 0.41378970465028864 92-93 0.4128334756643401 94-95 0.4212372573293112 96-97 0.4214763145757984 98-99 0.41858923859899216 100-101 0.3925503877508538 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2719014.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.848302557237304 #Duplication Level Percentage of deduplicated Percentage of total 1 73.0421023338149 37.871090212203235 2 14.224071744622691 14.74987950822095 3 5.364248370450316 8.343815175098253 4 2.4851347512031605 5.154000739035444 5 1.3595747528914608 3.5245821568548803 6 0.8409613305457382 2.6161450503043366 7 0.5495984608443529 1.9947023097994965 8 0.3642358229701287 1.5108007321231645 9 0.2758129890923955 1.2870391776910661 >10 1.3569396194052674 12.569724434738408 >50 0.0922856817599716 3.3026487367371486 >100 0.04076374832346565 3.762278558140629 >500 0.0029181026187113037 1.02495621680701 >1k 0.0011387717536251942 1.1801665083455914 >5k 7.117323460157464E-5 0.24499313495220226 >10k+ 1.4234646920314927E-4 0.8631773489481794 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12918 0.4750986938647612 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10473 0.3851763911476734 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6639 0.24416939375817853 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4270 0.15704222192309417 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3109 0.11434291989669784 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2933 0.10786998522258437 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2929 0.10772287307089995 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2789 0.10257394776194606 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.677803792109934E-5 0.0 12-13 0.0 0.0 0.0 3.677803792109934E-5 0.0 14-15 0.0 0.0 0.0 3.677803792109934E-5 0.0 16-17 0.0 0.0 0.0 5.5167056881649004E-5 0.0 18-19 0.0 0.0 0.0 9.194509480274835E-5 3.677803792109934E-5 20-21 0.0 0.0 0.0 1.6550117064494704E-4 3.677803792109934E-5 22-23 0.0 0.0 0.0 3.4939136025044374E-4 1.1033411376329802E-4 24-25 3.677803792109934E-5 0.0 0.0 6.803937015403378E-4 1.1033411376329802E-4 26-27 3.677803792109934E-5 0.0 0.0 0.001452732497883424 1.1033411376329802E-4 28-29 3.677803792109934E-5 0.0 0.0 0.003916861038597079 1.838901896054967E-4 30-31 3.677803792109934E-5 0.0 0.0 0.011198912546974749 1.838901896054967E-4 32-33 3.677803792109934E-5 0.0 0.0 0.025707848506848437 2.0227920856604636E-4 34-35 3.677803792109934E-5 0.0 0.0 0.048068895562876836 2.3905724648714573E-4 36-37 3.677803792109934E-5 0.0 0.0 0.08186791241236713 2.574462654476954E-4 38-39 3.677803792109934E-5 0.0 0.0 0.13289744002789247 2.7583528440824505E-4 40-41 3.677803792109934E-5 0.0 0.0 0.1908780168105056 2.942243033687947E-4 42-43 7.355607584219868E-5 0.0 0.0 0.2496125433705012 2.942243033687947E-4 44-45 7.355607584219868E-5 0.0 0.0 0.3224146694353174 3.677803792109934E-4 46-47 7.355607584219868E-5 0.0 0.0 0.41766978765096463 4.4133645505319203E-4 48-49 7.355607584219868E-5 0.0 0.0 0.5198759550336997 6.436156636192384E-4 50-51 7.355607584219868E-5 0.0 0.0 0.6265506540238484 8.275058532247351E-4 52-53 7.355607584219868E-5 0.0 0.0 0.7338873576965768 9.194509480274835E-4 54-55 7.355607584219868E-5 0.0 0.0 0.8577557894148393 9.194509480274835E-4 56-57 7.355607584219868E-5 0.0 0.0 1.0083986327396623 9.562289859485828E-4 58-59 7.355607584219868E-5 0.0 0.0 1.1798946235657484 0.0010297850617907816 60-61 7.355607584219868E-5 0.0 0.0 1.3688050153474753 0.0010849521186724305 62-63 7.355607584219868E-5 0.0 0.0 1.5550857774178435 0.0011952862324357285 64-65 7.355607584219868E-5 0.0 0.0 1.749145094508524 0.0012320642703568278 66-67 7.355607584219868E-5 0.0 0.0 1.9720016152914255 0.0012504532893173775 68-69 7.355607584219868E-5 0.0 0.0 2.230477665800912 0.0012504532893173775 70-71 9.194509480274835E-5 0.0 0.0 2.5127307178263885 0.0012504532893173775 72-73 1.1033411376329802E-4 0.0 0.0 2.8046563938251143 0.0012504532893173775 74-75 1.1033411376329802E-4 0.0 0.0 3.098880697193909 0.0012504532893173775 76-77 1.4711215168439735E-4 0.0 0.0 3.4222332065962147 0.0012504532893173775 78-79 1.4711215168439735E-4 0.0 0.0 3.791264039096525 0.0012504532893173775 80-81 1.4711215168439735E-4 0.0 0.0 4.191280368545362 0.0012504532893173775 82-83 1.4711215168439735E-4 0.0 0.0 4.595048057862151 0.0012504532893173775 84-85 1.4711215168439735E-4 0.0 0.0 5.011026055768745 0.0012504532893173775 86-87 1.4711215168439735E-4 0.0 0.0 5.44542985067381 0.0012504532893173775 88-89 1.4711215168439735E-4 0.0 0.0 5.916961074860225 0.0012504532893173775 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15190 0.0 25.340343 1 GTATCAA 21070 0.0 25.215204 1 TACATGG 15365 0.0 24.76451 2 ACATGGG 15445 0.0 24.02187 3 GGTATCA 15545 0.0 23.599989 1 GAGTACT 11250 0.0 22.615202 12-13 ATCAACG 24675 0.0 21.381372 3 TATCAAC 24745 0.0 21.359234 2 CATGGGA 9000 0.0 21.297411 4 TCAACGC 24850 0.0 21.249891 4 GTACTTT 11965 0.0 21.105669 14-15 CAACGCA 25140 0.0 21.004765 5 AACGCAG 25565 0.0 20.76501 6 AGTACTT 11555 0.0 20.744818 12-13 CATGGGG 6680 0.0 19.31881 4 ACGCAGA 27650 0.0 19.149128 7 ATGGGAT 3500 0.0 18.97789 5 CGCAGAG 27915 0.0 18.916006 8 ACTTTTT 13790 0.0 18.554821 16-17 TACTTTT 13270 0.0 18.207363 14-15 >>END_MODULE