FastQCFastQC Report
Thu 26 May 2016
SRR938692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719014
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120160.44192490365992965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111210.40900855972054573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72450.2664568847383647No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43470.15987413084301882No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31330.11522559280680424No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31170.11463714420006665No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30010.11037089180121912No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28530.10492774218889642No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166750.022.82081
TGTACGC2303.6725396E-922.695922
GTATAGG7850.022.4201661
GTACATG150550.022.4012471
GTATCAA216450.022.3496171
GTACGCT1754.085967E-621.6937643
TACATGG154000.021.6013162
TACCTGG20100.021.4846522
ACATGGG152250.020.9145343
GAGTACT120750.020.86231612-13
AGTACTT124650.019.94316912-13
GTACTTT127950.019.46588714-15
ATCAACG259150.018.5315723
TCAACGC260250.018.5079484
TATCAAC260600.018.464882
CAACGCA262850.018.3248735
AACGCAG267450.018.0398756
CATGGGA93550.017.906634
ATAGCGT2956.438313E-817.6899196
TACTTTT140200.017.68045614-15
Produced by FastQC (version 0.11.5)