FastQCFastQC Report
Thu 26 May 2016
SRR938691_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938691_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3340608
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121900.36490363430848516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96720.2895281338007932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62850.18813940456348066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69050.047.923191
GTATCAA116850.039.8010251
TCAACGC146300.031.4795514
ATCAACG146600.031.447453
TATCAAC147900.031.2671452
CAACGCA149400.030.826365
AACGCAG154700.029.8406726
ACGCAGA174650.026.1078727
CGCAGAG178100.025.4426218
GTACATG120550.024.1858771
TACATGG120650.023.2880952
GCAGAGT191150.023.235689
GTGGTAT37300.022.8779771
TGGTATC37550.022.3341752
ACATGGG122100.022.1578223
GAGTACT116000.021.18537712-13
GTGTAGC19850.020.3007641
CAGAGTA186750.020.06860510-11
GTACTTT121950.019.99672114-15
GTACCGT3404.3655746E-1119.4959076
Produced by FastQC (version 0.11.5)