##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938691_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3340608 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47890234352549 33.0 31.0 34.0 30.0 34.0 2 31.567002473801175 33.0 31.0 34.0 28.0 34.0 3 31.577861874245645 33.0 31.0 34.0 28.0 34.0 4 35.122908165220224 37.0 35.0 37.0 32.0 37.0 5 35.14463834128398 37.0 35.0 37.0 32.0 37.0 6 35.217680733567065 37.0 35.0 37.0 32.0 37.0 7 35.12023799260494 37.0 35.0 37.0 32.0 37.0 8 35.088608121635346 37.0 35.0 37.0 32.0 37.0 9 36.64528223604805 39.0 37.0 39.0 32.0 39.0 10-11 36.5305055247428 39.0 37.0 39.0 32.0 39.0 12-13 36.402291738509874 39.0 37.0 39.0 32.0 39.0 14-15 37.55479990468801 40.0 37.0 41.0 32.0 41.0 16-17 37.47135611242025 40.0 37.0 41.0 32.0 41.0 18-19 37.389550644673065 40.0 37.0 41.0 32.0 41.0 20-21 37.26916971401613 40.0 37.0 41.0 31.0 41.0 22-23 37.18298719873748 40.0 37.0 41.0 31.0 41.0 24-25 37.1957628671188 40.0 37.0 41.0 31.0 41.0 26-27 37.096969922840394 40.0 37.0 41.0 31.0 41.0 28-29 36.96606007050214 40.0 36.5 41.0 30.0 41.0 30-31 36.748657430024714 39.5 36.0 41.0 30.0 41.0 32-33 36.53533368775983 39.0 36.0 41.0 30.0 41.0 34-35 36.2072669106941 39.0 35.0 41.0 29.5 41.0 36-37 35.948672816445395 39.0 35.0 41.0 28.0 41.0 38-39 35.76818216923387 39.0 35.0 41.0 27.0 41.0 40-41 35.575345715510466 39.0 35.0 40.5 27.0 41.0 42-43 35.31380200849666 38.5 35.0 40.0 26.0 41.0 44-45 35.275018948646476 39.0 35.0 41.0 25.0 41.0 46-47 35.10307375184398 39.0 35.0 41.0 24.5 41.0 48-49 34.87155856059735 39.0 34.5 41.0 24.0 41.0 50-51 33.74022992221775 38.0 32.5 40.0 22.5 40.5 52-53 33.83301976765907 38.0 33.5 39.5 22.5 40.5 54-55 34.337153595992106 38.0 34.0 40.0 23.0 41.0 56-57 34.22441753117995 38.0 34.0 40.0 22.0 41.0 58-59 33.98933577360768 38.0 34.0 40.0 22.0 41.0 60-61 33.69708238739774 37.5 33.0 40.0 20.5 41.0 62-63 33.322809949566064 37.0 33.0 40.0 19.5 41.0 64-65 32.92702810386612 36.0 32.5 39.5 18.0 41.0 66-67 32.52969698929057 36.0 32.0 39.0 15.5 41.0 68-69 32.126602851935935 35.0 31.5 39.0 13.0 40.5 70-71 31.72748104536659 35.0 31.0 38.5 9.5 40.0 72-73 31.321967587936086 35.0 31.0 37.5 7.5 40.0 74-75 30.87631532942506 35.0 31.0 37.0 6.5 39.0 76-77 30.451532026505355 35.0 30.0 36.5 2.0 39.0 78-79 30.046972886372778 34.5 30.0 36.0 2.0 38.5 80-81 29.632115022175604 34.0 29.5 35.5 2.0 37.0 82-83 29.190904769431192 34.0 29.0 35.0 2.0 37.0 84-85 28.80456162471023 34.0 29.0 35.0 2.0 36.5 86-87 28.436742203814397 34.0 29.0 35.0 2.0 36.0 88-89 28.074465486522215 34.0 27.5 35.0 2.0 36.0 90-91 27.683656088951473 34.0 27.0 35.0 2.0 35.0 92-93 27.252110543948888 34.0 26.0 35.0 2.0 35.0 94-95 26.767397431844742 33.0 25.0 35.0 2.0 35.0 96-97 26.197892120236794 33.0 24.5 35.0 2.0 35.0 98-99 25.50610951659099 33.0 21.0 35.0 2.0 35.0 100-101 23.288275068490528 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18806376339806619 1101 2 0.028599600360593058 1101 3 0.3064350025229601 1101 4 0.2860468901263715 1101 5 0.06592321541906898 1101 6 0.04653027768291906 1101 7 0.19969154895254349 1101 8 0.3038910818067393 1101 9 0.3549596203320178 1101 10-11 0.4067865812125433 1101 12-13 0.34533908683095405 1101 14-15 0.550896659585554 1101 16-17 0.4657852719531519 1101 18-19 0.5545936715952067 1101 20-21 0.5033453471754186 1101 22-23 0.5415113061011638 1101 24-25 0.515592027633069 1101 26-27 0.4876338948087593 1101 28-29 0.4284668719402802 1101 30-31 0.4720993063765917 1101 32-33 0.31271470161734527 1101 34-35 0.3869346182935658 1101 36-37 0.42692635415473745 1101 38-39 0.4598421942117312 1101 40-41 0.5078659265622179 1101 42-43 0.47432967704094153 1101 44-45 0.5692263727791129 1101 46-47 0.6609460402356859 1101 48-49 0.49439498192811016 1101 50-51 0.3994195051840421 1101 52-53 0.45739798534035714 1101 54-55 0.41807235006477583 1101 56-57 0.2926947037307457 1101 58-59 0.28126663256004036 1101 60-61 0.20542908532296877 1101 62-63 0.2837990328121336 1101 64-65 0.3007535321177741 1101 66-67 0.0822176012081286 1101 68-69 -0.06487848849378253 1101 70-71 -0.1155120251062165 1101 72-73 -0.1249724723982446 1101 74-75 -0.17630404419502455 1101 76-77 -0.13581905940905514 1101 78-79 -0.2837047571535898 1101 80-81 -0.24706395218253974 1101 82-83 -0.13957377506191193 1101 84-85 -0.10048529033853626 1101 86-87 -0.24817930165338709 1101 88-89 -0.35403442329270973 1101 90-91 -0.3667485868706635 1101 92-93 -0.3500074240459732 1101 94-95 -0.5567785612706402 1101 96-97 -0.6426509051257696 1101 98-99 -0.5773346140847728 1101 100-101 -0.45588269932528647 1102 1 0.285531763222469 1102 2 0.34157798466329936 1102 3 0.3700681599356237 1102 4 0.3684943576053499 1102 5 0.33655135860902163 1102 6 0.3605391855734581 1102 7 0.4322195237616313 1102 8 0.3498516897635753 1102 9 0.4709283087842735 1102 10-11 0.3489026851339645 1102 12-13 0.4357236321021887 1102 14-15 0.3585061834092542 1102 16-17 0.5089622571247716 1102 18-19 0.49420536137858306 1102 20-21 0.4704629950220749 1102 22-23 0.4323410040796247 1102 24-25 0.4318815177061879 1102 26-27 0.396550955889424 1102 28-29 0.43689735475360436 1102 30-31 0.39675342992185847 1102 32-33 0.30730715000001396 1102 34-35 0.42614566878029336 1102 36-37 0.5004485386861646 1102 38-39 0.5907400816011759 1102 40-41 0.575093453521383 1102 42-43 0.49752882777045215 1102 44-45 0.6559004750888988 1102 46-47 0.5021380770041688 1102 48-49 0.3644722786378054 1102 50-51 0.4258089076812581 1102 52-53 0.44358608602958327 1102 54-55 0.40165149613647344 1102 56-57 0.32088036092577 1102 58-59 0.18688508808420323 1102 60-61 0.20428527854651435 1102 62-63 0.15352048265837226 1102 64-65 0.07205458488876815 1102 66-67 0.17891949789237316 1102 68-69 0.1792267863732775 1102 70-71 0.11319817603561688 1102 72-73 0.18143706809240356 1102 74-75 0.0430440175649629 1102 76-77 0.051086854903470424 1102 78-79 -0.12388989425042851 1102 80-81 -0.20141901183013644 1102 82-83 -0.19080186527476428 1102 84-85 -0.1415281244048927 1102 86-87 -0.31463402892922687 1102 88-89 -0.32419974234206705 1102 90-91 -0.2475250064076029 1102 92-93 -0.35907212530202415 1102 94-95 -0.38088408911821503 1102 96-97 -0.2971314133487546 1102 98-99 -0.338633170533285 1102 100-101 -0.09727760414880748 1103 1 0.2170592587787361 1103 2 0.2629976129950933 1103 3 0.21082898240172554 1103 4 0.33672766294100853 1103 5 0.25419523185378523 1103 6 0.22637715400275482 1103 7 0.1888073264537269 1103 8 0.23989451267505757 1103 9 0.3597914082035629 1103 10-11 0.2558808250084894 1103 12-13 0.29782789680489685 1103 14-15 0.38849232291518376 1103 16-17 0.4644857673090641 1103 18-19 0.5807279552088787 1103 20-21 0.4353422929910664 1103 22-23 0.45825190967943996 1103 24-25 0.4136075505333068 1103 26-27 0.35017868104612404 1103 28-29 0.4796252393032461 1103 30-31 0.40578757050723624 1103 32-33 0.38178618849382673 1103 34-35 0.5083647629501087 1103 36-37 0.5459287761922482 1103 38-39 0.517182744936882 1103 40-41 0.5695647052002357 1103 42-43 0.5630748774839347 1103 44-45 0.6282132194667227 1103 46-47 0.626798786516666 1103 48-49 0.5492592574157626 1103 50-51 0.634492440478212 1103 52-53 0.5456170844641761 1103 54-55 0.5137857452874925 1103 56-57 0.5696390687569348 1103 58-59 0.5390543075122309 1103 60-61 0.6049305292475751 1103 62-63 0.5270868969315572 1103 64-65 0.4865776529852752 1103 66-67 0.45983497246391636 1103 68-69 0.34989419814129263 1103 70-71 0.3223236137811405 1103 72-73 0.4175579708729522 1103 74-75 0.46770150923422094 1103 76-77 0.3732636271214851 1103 78-79 0.3091479764734508 1103 80-81 0.272335180050586 1103 82-83 0.25439849300282447 1103 84-85 0.29413416258211456 1103 86-87 0.362972680054515 1103 88-89 0.22645774896152915 1103 90-91 0.16318907688553708 1103 92-93 0.18201589331466295 1103 94-95 0.10654579937985886 1103 96-97 0.08126391405034283 1103 98-99 0.11742809427656553 1103 100-101 0.2676558943671452 1104 1 0.09040847608411795 1104 2 0.09795620271835404 1104 3 0.17216889834831406 1104 4 0.26613386644586257 1104 5 0.22134433625699756 1104 6 0.18615776813376783 1104 7 0.07067630693047988 1104 8 0.15526374356813477 1104 9 0.15920345219326038 1104 10-11 0.12065876673552367 1104 12-13 0.14059452823448026 1104 14-15 0.24948163791233213 1104 16-17 0.2359930112921731 1104 18-19 0.25354864345985817 1104 20-21 0.2103548680786531 1104 22-23 0.31559424575940653 1104 24-25 0.22459355951030346 1104 26-27 0.23378524420616742 1104 28-29 0.1149946438641436 1104 30-31 0.11191209268330482 1104 32-33 0.18951108673308426 1104 34-35 0.1858080452573745 1104 36-37 0.24351594228534879 1104 38-39 0.13885966328108879 1104 40-41 0.16046863623457597 1104 42-43 0.3078901017112514 1104 44-45 0.3571504113250654 1104 46-47 0.3579589716501772 1104 48-49 0.3346336956297762 1104 50-51 0.39135460313639214 1104 52-53 0.3821909205573988 1104 54-55 0.43685289259190796 1104 56-57 0.4454598866256205 1104 58-59 0.5148398581509142 1104 60-61 0.5038161969401855 1104 62-63 0.5055506404733947 1104 64-65 0.3381253181526418 1104 66-67 0.3800665404066095 1104 68-69 0.3199174596789689 1104 70-71 0.4102877195379655 1104 72-73 0.294693146574577 1104 74-75 0.1499314655033963 1104 76-77 0.049506521100273204 1104 78-79 0.23241276137093436 1104 80-81 0.2803662763585244 1104 82-83 0.22415062470743052 1104 84-85 0.3777367639450979 1104 86-87 0.2817791317053526 1104 88-89 0.29635633147938734 1104 90-91 0.1381884640733233 1104 92-93 0.10699524086457401 1104 94-95 0.04291065520074966 1104 96-97 0.026929405052221966 1104 98-99 0.12748006889320251 1104 100-101 0.3885632472001781 1105 1 0.24459115950803678 1105 2 0.23940191171416814 1105 3 0.2757693349083006 1105 4 0.3436683220065859 1105 5 0.2707190341649479 1105 6 0.3099559310500126 1105 7 0.25523195057883186 1105 8 0.21548162304808471 1105 9 0.37351799566675226 1105 10-11 0.3294371633879578 1105 12-13 0.4078352342674023 1105 14-15 0.5314957032270229 1105 16-17 0.32833983071235906 1105 18-19 0.25721156926742594 1105 20-21 0.3830286341549538 1105 22-23 0.4138511917146346 1105 24-25 0.3962086038990549 1105 26-27 0.4131568745428993 1105 28-29 0.47196189913430686 1105 30-31 0.3602355330235909 1105 32-33 0.3947297353814818 1105 34-35 0.31354148333111453 1105 36-37 0.307714473310142 1105 38-39 0.2942914944754662 1105 40-41 0.2618208252697869 1105 42-43 0.4084896890000351 1105 44-45 0.4762039882314397 1105 46-47 0.43046529567126157 1105 48-49 0.36070973498570424 1105 50-51 0.4211373517348207 1105 52-53 0.5058602818927085 1105 54-55 0.5259170922152379 1105 56-57 0.46548084077681295 1105 58-59 0.4484520188406762 1105 60-61 0.4838814897840038 1105 62-63 0.41845005053485096 1105 64-65 0.367620470570877 1105 66-67 0.2368546112866241 1105 68-69 0.15473184431234444 1105 70-71 0.21980624739505927 1105 72-73 0.24841005071252553 1105 74-75 0.2825239090832987 1105 76-77 0.28501975671431623 1105 78-79 0.13559893927732247 1105 80-81 0.22128430848882985 1105 82-83 0.14784647456574973 1105 84-85 0.14506612915811345 1105 86-87 0.1558828228946858 1105 88-89 0.054853048632026 1105 90-91 0.15198520698627505 1105 92-93 0.22631688890583845 1105 94-95 0.35424706035317755 1105 96-97 0.4921133364405783 1105 98-99 0.5261167291910951 1105 100-101 0.5254384116156245 1106 1 0.17628750980331276 1106 2 0.18858006948400785 1106 3 0.20301695062487823 1106 4 0.2641045386956975 1106 5 0.21000185346247235 1106 6 0.2210209890081387 1106 7 0.15145611223549338 1106 8 0.21822984877769613 1106 9 0.30808328909265725 1106 10-11 0.3498725079741689 1106 12-13 0.2888057654515066 1106 14-15 0.245449211880576 1106 16-17 0.2605280470416176 1106 18-19 0.32193312517242845 1106 20-21 0.32495713824145156 1106 22-23 0.309583028335787 1106 24-25 0.29511526226697526 1106 26-27 0.25673209249976736 1106 28-29 0.34632335916308676 1106 30-31 0.2792579332160088 1106 32-33 0.3937267711496375 1106 34-35 0.41394994878803715 1106 36-37 0.46752485060022053 1106 38-39 0.2798138519807267 1106 40-41 0.4321800674031877 1106 42-43 0.493584073483575 1106 44-45 0.5277296174995882 1106 46-47 0.4640019207799284 1106 48-49 0.549976122028653 1106 50-51 0.5154561980620258 1106 52-53 0.5164350344220381 1106 54-55 0.4755094988612072 1106 56-57 0.5887308286132722 1106 58-59 0.5213112127789188 1106 60-61 0.5832061608405539 1106 62-63 0.5198157177715999 1106 64-65 0.4759797604404383 1106 66-67 0.21274551029618038 1106 68-69 0.4720528176526173 1106 70-71 0.5053002845062515 1106 72-73 0.41063165481269337 1106 74-75 0.33291089326886336 1106 76-77 0.2151558231263273 1106 78-79 0.5415655000982369 1106 80-81 0.3459249791451384 1106 82-83 0.3135571328565554 1106 84-85 0.44543268777582057 1106 86-87 0.6447701205782899 1106 88-89 0.6124532876800188 1106 90-91 0.5246526300874557 1106 92-93 0.6959493021247134 1106 94-95 0.6774917696721019 1106 96-97 0.77488668791365 1106 98-99 0.813936251627549 1106 100-101 1.04258904949004 1107 1 -6.05034477665356E-4 1107 2 0.10068605768664796 1107 3 0.04182996560117758 1107 4 0.0931853447532589 1107 5 0.07428615648757386 1107 6 0.05554591294119149 1107 7 -0.14230687501196115 1107 8 0.0097613118111326 1107 9 0.022186026140154524 1107 10-11 0.018417650113420336 1107 12-13 0.14234722176734493 1107 14-15 0.12180748209163283 1107 16-17 0.09211766569174529 1107 18-19 0.22090518240693058 1107 20-21 0.2279498088351133 1107 22-23 0.2414041521235859 1107 24-25 0.14097985015649783 1107 26-27 0.14864249360729076 1107 28-29 0.02080424397870928 1107 30-31 0.15915622901662374 1107 32-33 0.29636652926151896 1107 34-35 0.22004688662502758 1107 36-37 0.27064015487481896 1107 38-39 0.35440646970565837 1107 40-41 0.2948028203113111 1107 42-43 0.19315499185996288 1107 44-45 0.3099074009094238 1107 46-47 0.34525695387058164 1107 48-49 0.2840572482963708 1107 50-51 0.44664098930543616 1107 52-53 0.24787817395327494 1107 54-55 0.3342830530441816 1107 56-57 0.2502098180572361 1107 58-59 0.10923738185260845 1107 60-61 0.09624539135509025 1107 62-63 0.15959072584498557 1107 64-65 0.137798036401648 1107 66-67 0.11938953060977298 1107 68-69 0.18636403195770157 1107 70-71 0.2900032316661232 1107 72-73 0.09715530335546418 1107 74-75 0.23360840639407243 1107 76-77 0.29729695269153567 1107 78-79 0.21720922472839632 1107 80-81 0.18174791246103084 1107 82-83 0.2866729685437761 1107 84-85 0.15834247162714377 1107 86-87 0.3094036570747569 1107 88-89 0.296664337519708 1107 90-91 0.3101915301723466 1107 92-93 0.2919040257697141 1107 94-95 0.36600944186682227 1107 96-97 0.2333492975856295 1107 98-99 0.4670027068192475 1107 100-101 0.3893280155246046 1108 1 0.2261332012196604 1108 2 0.12317520177199626 1108 3 0.06502069424672996 1108 4 0.06321072187259347 1108 5 0.11475571915213578 1108 6 0.057825313394069155 1108 7 0.14262161987404198 1108 8 0.07134470828972184 1108 9 0.04358885143299318 1108 10-11 0.18333881882740144 1108 12-13 0.17213864626700115 1108 14-15 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#Quality Count 2 16042.0 3 5391.0 4 5247.0 5 5701.0 6 6193.0 7 7162.0 8 8738.0 9 10367.0 10 12104.0 11 14828.0 12 17588.0 13 21299.0 14 22692.0 15 21609.0 16 25155.0 17 29957.0 18 26660.0 19 22153.0 20 22405.0 21 24052.0 22 26045.0 23 29236.0 24 33143.0 25 37624.0 26 43391.0 27 51152.0 28 58871.0 29 69911.0 30 83562.0 31 101437.0 32 124142.0 33 158657.0 34 210061.0 35 297399.0 36 434824.0 37 621902.0 38 541413.0 39 92464.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.487796439221455 18.706290037171645 14.453707540285274 23.352205983321632 2 15.553597993698848 19.773302351920684 40.527433082096245 24.14566657228422 3 19.41659610956215 23.95828651996781 29.4932292144163 27.13188815605374 4 13.16455650685547 16.297044098204836 36.61504835807872 33.92335103686097 5 14.215756061551076 36.62973054544594 34.073084786036546 15.08142860696644 6 32.52064633247229 35.35486417351484 17.353340516402373 14.771148977610505 7 28.74282842790515 32.18482366871844 20.63963325632946 18.43271464704695 8 26.206076382441694 35.05443994954317 19.600837592974376 19.13864607504076 9 26.323877121657947 16.954695536937624 20.286696165854316 36.43473117555012 10-11 25.22660849580834 26.233742392102847 27.012717987235597 21.526931124853217 12-13 26.062822748444702 23.557924197307724 27.53095801958522 22.84829503466235 14-15 23.06094441037805 25.0175086307779 25.813778846938558 26.107768111905493 16-17 22.616613102307053 28.10162049719845 26.465280846400237 22.816485554094264 18-19 22.447735786445985 26.64315860216664 28.831271436084382 22.077834175302996 20-21 23.145088831119047 25.857148419249338 28.91813820037617 22.079624549255445 22-23 22.858126199839628 25.794653653907616 28.786176409711405 22.561043736541354 24-25 22.698011796890484 25.98517007705562 28.88740226491789 22.429415861136007 26-27 22.423918318922905 26.21255834576645 28.79601001839502 22.567513316915626 28-29 22.37811801784705 26.33315544297321 28.823106235294276 22.465620303885466 30-31 22.589980765823643 26.111304223326716 28.709722686698285 22.58899232415136 32-33 22.304929799556085 26.21961647098075 28.673973977961264 22.801479751501898 34-35 22.874642186619727 26.315955388182765 28.46291823203375 22.34648419316376 36-37 22.861337480896644 26.033197849807422 28.515630580349587 22.589834088946343 38-39 22.586021094765275 26.271937130647665 28.545902673499 22.596139101088053 40-41 22.855730417331515 26.17824277542834 28.495318292388184 22.47070851485196 42-43 22.689488397939243 26.293107984699176 28.266948282296013 22.750455335065567 44-45 22.703686005245316 26.382323390130015 28.264225308570506 22.649765296054163 46-47 22.882351517145427 26.502203051206752 27.86478229090561 22.750663140742216 48-49 22.845022442403646 26.640169300813028 27.733986458461473 22.780821798321853 50-51 22.787924537380945 26.882911439991098 27.602816765861977 22.726347256765983 52-53 23.042974872787934 26.688886138565472 27.582667729167063 22.68547125947953 54-55 22.783574820241896 26.626890170496903 27.73104656824342 22.85848844101778 56-57 22.739203308850005 26.93499457359784 27.52455486439106 22.80124725316109 58-59 22.785518837190253 26.593433681111105 27.629853025501284 22.991194456197363 60-61 22.7710232382372 26.646582106159173 27.826141833560097 22.756252822043532 62-63 22.639845407455486 26.69789970097535 27.888420458524514 22.77383443304465 64-65 22.55822995087124 26.65064887112142 27.769608451379973 23.021512726627368 66-67 22.57226323350098 26.73530952531716 27.608741721894148 23.083685519287716 68-69 22.634422924448735 26.614835765608124 27.734641350090378 23.016099959852763 70-71 22.618043052189986 26.522748228470522 27.628444348177933 23.230764371161563 72-73 22.713333289215708 26.592746580934083 27.557012506745487 23.136907623104722 74-75 22.58857464693838 26.834548116465783 27.4273893554769 23.149487881118937 76-77 22.676943313543486 26.645007298646668 27.522694370448868 23.155355017360975 78-79 22.678156556640825 26.696706740977643 27.454242753364476 23.170893949017056 80-81 22.672323204382515 26.77344333715681 27.37364869232654 23.180584766134135 82-83 22.74599342096064 26.79267846948553 27.36614553662592 23.09518257292791 84-85 22.690327435431165 26.681405784038603 27.358407874627794 23.26985890590244 86-87 22.700233890957644 26.67077159957808 27.413854674849468 23.21513983461481 88-89 22.707424565810445 26.561899653267922 27.315891914392047 23.41478386652958 90-91 22.676224306261542 26.66471663949662 27.339235549245956 23.319823504995878 92-93 22.77877319431657 26.504211078168954 27.34000504709772 23.37701068041675 94-95 22.664878342091974 26.363193922022592 27.431541389196116 23.540386346689317 96-97 22.731638397686023 26.209583298741585 27.47901032699131 23.579767976581085 98-99 22.62432503359323 26.211683601765873 27.31740659502617 23.846584769614726 100-101 22.903103037145293 25.815516945047968 27.605062695752714 23.67631732205403 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3334.0 1 2009.0 2 934.5 3 1193.0 4 969.0 5 916.5 6 1584.0 7 3183.5 8 5121.5 9 5794.0 10 4770.5 11 3754.0 12 3649.5 13 3919.5 14 4305.0 15 4543.0 16 4737.5 17 5062.0 18 5410.5 19 5689.5 20 6089.0 21 6841.0 22 7964.5 23 9560.5 24 11648.0 25 14296.0 26 17996.0 27 22478.5 28 26500.5 29 30858.5 30 36322.0 31 42359.0 32 48872.0 33 53702.5 34 58330.0 35 63457.5 36 69439.5 37 76182.0 38 81299.5 39 85105.5 40 88398.5 41 93025.0 42 97479.5 43 102653.0 44 108239.0 45 113118.0 46 115927.0 47 118837.5 48 124876.0 49 126898.5 50 125240.0 51 123752.0 52 121643.5 53 120869.0 54 119670.5 55 113517.5 56 104528.0 57 94729.5 58 86867.0 59 78513.5 60 66888.5 61 56036.5 62 46429.5 63 36431.0 64 27606.5 65 21322.5 66 16073.0 67 12443.0 68 9597.5 69 7095.0 70 5440.5 71 4078.0 72 3148.5 73 2681.5 74 2148.0 75 1671.0 76 1253.0 77 851.5 78 610.0 79 401.5 80 281.5 81 209.0 82 147.0 83 112.0 84 79.0 85 55.0 86 37.0 87 27.5 88 27.0 89 25.5 90 26.0 91 19.5 92 12.0 93 11.0 94 9.0 95 7.0 96 7.5 97 4.5 98 3.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15775571392991936 2 0.09348597620552905 3 0.08639145927926892 4 0.10237657336628543 5 0.12354038546276606 6 0.038136770312470064 7 0.041369714734563294 8 0.04538096059160488 9 0.045889849991378816 10-11 0.044273377780332204 12-13 0.04820978696093645 14-15 0.046114360020690845 16-17 0.044258410445044735 18-19 0.04825468896679886 20-21 0.05052972393049409 22-23 0.06409012970094066 24-25 0.08065896986416843 26-27 0.08226047473992758 28-29 0.07212758875031132 30-31 0.06045306722608579 32-33 0.04996096518957028 34-35 0.29295565358162345 36-37 0.46708563231603345 38-39 0.444649596720118 40-41 0.4204025135544188 42-43 0.45006777209418164 44-45 0.45964686667816157 46-47 0.4590032712608005 48-49 0.47521289537712896 50-51 0.4645262179818764 52-53 0.4713662902082495 54-55 0.46234098702990595 56-57 0.4412220769392877 58-59 0.4193997020901585 60-61 0.3894650315152212 62-63 0.4478975084774987 64-65 0.4843579372377723 66-67 0.4864832988485928 68-69 0.49672095618522133 70-71 0.4940118684981895 72-73 0.48380414583213593 74-75 0.47717361629978733 76-77 0.45901823859608787 78-79 0.433918017319003 80-81 0.438932074640305 82-83 0.4504120208057934 84-85 0.448735679253597 86-87 0.459212813954825 88-89 0.47016890338525197 90-91 0.4750033526831044 92-93 0.4767096289058758 94-95 0.48137943751556606 96-97 0.48242715098568884 98-99 0.47612590282966455 100-101 0.46286484376496734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3340608.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.91717339178529 #Duplication Level Percentage of deduplicated Percentage of total 1 74.77260132099158 41.06299911699729 2 13.938981331648225 15.309789093899678 3 4.825847327474198 7.950656833355525 4 2.18070042559056 4.7903161355078705 5 1.1550229107820367 3.1715296731450833 6 0.7053524446459012 2.3241577502963153 7 0.4765362365872246 1.83190161924906 8 0.30641278090451857 1.3461859054714052 9 0.24890373084592265 1.2302180410255013 >10 1.2700647106497405 12.640585681301445 >50 0.08392632663534287 3.127024961030921 >100 0.033141127955751165 3.2739206529816185 >500 0.0017456175942127388 0.6402542548256802 >1k 6.000560458858472E-4 0.45724315290420436 >5k 1.0910109925197221E-4 0.4780337411316509 >10k+ 5.4550549625986106E-5 0.36518338687690827 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12190 0.36490363430848516 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9672 0.2895281338007932 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6285 0.18813940456348066 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.7960802344962353E-4 0.0 0.0 0.0 2.9934670574937258E-5 7 1.7960802344962353E-4 0.0 0.0 0.0 2.9934670574937258E-5 8 1.7960802344962353E-4 0.0 0.0 0.0 2.9934670574937258E-5 9 1.7960802344962353E-4 0.0 0.0 0.0 2.9934670574937258E-5 10-11 1.9457535873709217E-4 0.0 0.0 0.0 2.9934670574937258E-5 12-13 2.095426940245608E-4 0.0 0.0 0.0 4.490200586240589E-5 14-15 2.095426940245608E-4 0.0 0.0 2.9934670574937258E-5 7.483667643734314E-5 16-17 2.694120351744353E-4 0.0 0.0 1.4967335287468627E-4 8.980401172481176E-5 18-19 3.143140410368412E-4 0.0 0.0 1.646406881621549E-4 1.1973868229974903E-4 20-21 3.2928137632430983E-4 0.0 0.0 2.544446998869667E-4 1.646406881621549E-4 22-23 3.2928137632430983E-4 0.0 0.0 3.2928137632430983E-4 2.2451002931202945E-4 24-25 3.5921604689924706E-4 0.0 0.0 3.8915071747418434E-4 2.694120351744353E-4 26-27 3.5921604689924706E-4 0.0 0.0 6.585627526486197E-4 2.694120351744353E-4 28-29 3.741833821867157E-4 0.0 0.0 0.0027240550223192905 2.694120351744353E-4 30-31 4.190853880491216E-4 0.0 0.0 0.008666087131444336 2.694120351744353E-4 32-33 4.190853880491216E-4 0.0 0.0 0.019846686591183403 2.9934670574937255E-4 34-35 4.4902005862405885E-4 0.0 0.0 0.03681964480717283 3.2928137632430983E-4 36-37 4.7895472919899613E-4 0.0 0.0 0.06798163687568251 3.2928137632430983E-4 38-39 5.23856735061402E-4 0.0 0.0 0.1126441653734889 3.442487116117784E-4 40-41 5.388240703488706E-4 0.0 0.0 0.1598661082054524 3.8915071747418434E-4 42-43 6.136607467862137E-4 1.4967335287468629E-5 0.0 0.20861471923673774 3.8915071747418434E-4 44-45 6.286280820736824E-4 2.9934670574937258E-5 0.0 0.26945693718029773 3.8915071747418434E-4 46-47 6.884974232235569E-4 2.9934670574937258E-5 0.0 0.3518371506025251 3.8915071747418434E-4 48-49 6.884974232235569E-4 2.9934670574937258E-5 0.0 0.43905181332260473 3.8915071747418434E-4 50-51 7.333994290859628E-4 2.9934670574937258E-5 0.0 0.5299484405234017 3.8915071747418434E-4 52-53 7.483667643734314E-4 2.9934670574937258E-5 0.0 0.6205906230243117 5.537914056363393E-4 54-55 7.783014349483687E-4 2.9934670574937258E-5 0.0 0.7258259574305037 6.286280820736824E-4 56-57 8.232034408107746E-4 2.9934670574937258E-5 0.0 0.8517012471981149 6.286280820736824E-4 58-59 8.681054466731804E-4 2.9934670574937258E-5 0.0 0.9924241335708949 6.286280820736824E-4 60-61 8.980401172481177E-4 2.9934670574937258E-5 0.0 1.1418879437515566 6.286280820736824E-4 62-63 8.980401172481177E-4 2.9934670574937258E-5 0.0 1.2966052886181196 6.286280820736824E-4 64-65 8.980401172481177E-4 2.9934670574937258E-5 0.0 1.462757677644309 6.286280820736824E-4 66-67 8.980401172481177E-4 2.9934670574937258E-5 0.0 1.652977541812748 6.286280820736824E-4 68-69 9.27974787823055E-4 4.490200586240589E-5 0.0 1.8750927974787823 6.286280820736824E-4 70-71 9.579094583979922E-4 5.9869341149874516E-5 0.0 2.1165159156656514 6.585627526486197E-4 72-73 0.0010177787995478667 5.9869341149874516E-5 0.0 2.3619802143801367 7.483667643734314E-4 74-75 0.001047713470122804 5.9869341149874516E-5 0.0 2.61467373603847 7.483667643734314E-4 76-77 0.001047713470122804 5.9869341149874516E-5 0.0 2.888815449163745 7.483667643734314E-4 78-79 0.0010626808054102726 5.9869341149874516E-5 0.0 3.2051201457938197 7.483667643734314E-4 80-81 0.0011375174818476158 5.9869341149874516E-5 0.0 3.5490994453704237 7.483667643734314E-4 82-83 0.0011375174818476158 5.9869341149874516E-5 0.0 3.8995596011265015 8.08236105523306E-4 84-85 0.0012422888288598961 5.9869341149874516E-5 0.0 4.259568916795985 8.08236105523306E-4 86-87 0.001287190834722302 5.9869341149874516E-5 0.0 4.635129293829147 8.08236105523306E-4 88-89 0.001287190834722302 5.9869341149874516E-5 0.0 5.047329707646034 8.381707760982432E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6905 0.0 47.92319 1 GTATCAA 11685 0.0 39.801025 1 TCAACGC 14630 0.0 31.479551 4 ATCAACG 14660 0.0 31.44745 3 TATCAAC 14790 0.0 31.267145 2 CAACGCA 14940 0.0 30.82636 5 AACGCAG 15470 0.0 29.840672 6 ACGCAGA 17465 0.0 26.107872 7 CGCAGAG 17810 0.0 25.442621 8 GTACATG 12055 0.0 24.185877 1 TACATGG 12065 0.0 23.288095 2 GCAGAGT 19115 0.0 23.23568 9 GTGGTAT 3730 0.0 22.877977 1 TGGTATC 3755 0.0 22.334175 2 ACATGGG 12210 0.0 22.157822 3 GAGTACT 11600 0.0 21.185377 12-13 GTGTAGC 1985 0.0 20.300764 1 CAGAGTA 18675 0.0 20.068605 10-11 GTACTTT 12195 0.0 19.996721 14-15 GTACCGT 340 4.3655746E-11 19.495907 6 >>END_MODULE