FastQCFastQC Report
Thu 26 May 2016
SRR938691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938691_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3340608
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117250.35098401249113936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107670.32230659808034945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70370.21065027683583348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76750.047.1682781
GTATCAA123700.036.197031
ATCAACG159450.027.914243
TCAACGC159800.027.8827974
TATCAAC160850.027.7597852
CAACGCA163150.027.2811875
AACGCAG169050.026.329056
ACGCAGA191950.022.965467
CGCAGAG194200.022.626088
GTACATG120250.022.1036451
TACATGG120850.021.124342
GCAGAGT210450.020.5633329
GAGTACT123250.020.48260912-13
ACATGGG122350.020.4387443
GTGGTAT44200.019.9372041
GTACTTT126200.019.85340914-15
TGGTATC42450.019.3381962
AGAGTAC185400.019.19605810-11
AGTACTT126500.018.81225812-13
CAGAGTA205700.018.7088510-11
Produced by FastQC (version 0.11.5)