FastQCFastQC Report
Thu 26 May 2016
SRR938690_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938690_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3353380
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119830.35734095151757334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96900.2889621814408149No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64510.19237306836684182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.044.7086831
GTATCAA118000.039.7916531
ATCAACG148950.031.3127843
TCAACGC150500.030.9587694
TATCAAC152400.030.6968732
CAACGCA152200.030.4882775
AACGCAG157700.029.543546
ACGCAGA177300.026.1170317
CGCAGAG180150.025.625238
GCAGAGT194150.023.3616339
GTACATG122950.022.4963191
TACATGG123550.022.1198372
GAGTACT110050.021.61176312-13
GTGGTAT41150.021.0193141
ACATGGG125300.020.9402943
AGAGTAC170350.020.63367810-11
AGTACTT116050.020.33076712-13
TGGTATC40900.020.0689662
GTACTTT118500.020.03075814-15
CAGAGTA187750.019.7453510-11
Produced by FastQC (version 0.11.5)