##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938690_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3353380 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.441879834674268 33.0 31.0 34.0 30.0 34.0 2 31.535741252109812 33.0 31.0 34.0 28.0 34.0 3 31.544978797511764 33.0 31.0 34.0 28.0 34.0 4 35.08951595107026 37.0 35.0 37.0 32.0 37.0 5 35.106900202184065 37.0 35.0 37.0 32.0 37.0 6 35.153848355987094 37.0 35.0 37.0 32.0 37.0 7 35.05475758786657 37.0 35.0 37.0 32.0 37.0 8 35.01849477243856 37.0 35.0 37.0 32.0 37.0 9 36.58360788219647 39.0 37.0 39.0 32.0 39.0 10-11 36.46036968670416 39.0 37.0 39.0 32.0 39.0 12-13 36.3343544423835 39.0 37.0 39.0 32.0 39.0 14-15 37.48661932140109 40.0 37.0 41.0 32.0 41.0 16-17 37.355526066237644 40.0 37.0 41.0 32.0 41.0 18-19 37.27972061024995 40.0 37.0 41.0 31.5 41.0 20-21 37.15570677942851 40.0 37.0 41.0 31.0 41.0 22-23 37.17488996176992 40.0 37.0 41.0 31.0 41.0 24-25 37.10835500301188 40.0 37.0 41.0 31.0 41.0 26-27 36.96814169584121 40.0 37.0 41.0 30.0 41.0 28-29 36.79037061114458 40.0 36.0 41.0 30.0 41.0 30-31 36.55265567874801 39.0 36.0 41.0 30.0 41.0 32-33 36.35824049168302 39.0 35.5 41.0 30.0 41.0 34-35 36.067272870954085 39.0 35.0 41.0 28.5 41.0 36-37 35.85296193691141 39.0 35.0 41.0 27.5 41.0 38-39 35.67605073090434 39.0 35.0 41.0 27.0 41.0 40-41 35.55373354645164 39.0 35.0 40.5 27.0 41.0 42-43 35.548387895198275 39.0 35.0 41.0 26.5 41.0 44-45 35.368815195414776 39.0 35.0 41.0 25.5 41.0 46-47 35.076217279282396 39.0 35.0 41.0 24.0 41.0 48-49 34.78777278447417 39.0 34.0 41.0 24.0 41.0 50-51 33.662267026105 38.0 32.5 39.5 22.0 40.5 52-53 33.73525830654444 38.0 33.0 39.5 22.0 40.5 54-55 34.212305047444666 38.0 34.0 40.0 22.0 41.0 56-57 34.09265532090011 38.0 34.0 40.0 22.0 41.0 58-59 33.86183641579541 38.0 33.5 40.0 21.0 41.0 60-61 33.54388273920641 37.5 33.0 40.0 19.5 41.0 62-63 33.132298158872544 37.0 33.0 40.0 18.0 41.0 64-65 32.73024321729121 36.0 32.0 39.5 15.5 41.0 66-67 32.341324275805306 36.0 32.0 39.0 13.0 41.0 68-69 31.913396632651235 35.0 31.0 39.0 9.0 40.5 70-71 31.492490114451687 35.0 31.0 38.0 7.5 40.0 72-73 31.06780904639498 35.0 31.0 37.5 6.0 40.0 74-75 30.604066494104455 35.0 30.0 37.0 2.0 39.0 76-77 30.148892609844395 34.5 30.0 36.0 2.0 39.0 78-79 29.726837101670554 34.0 29.0 36.0 2.0 38.5 80-81 29.267710190911856 34.0 29.0 35.0 2.0 37.0 82-83 28.85637550769671 34.0 29.0 35.0 2.0 37.0 84-85 28.501971145530778 34.0 29.0 35.0 2.0 36.5 86-87 28.080334617609694 34.0 27.5 35.0 2.0 36.0 88-89 27.632939601238157 34.0 26.5 35.0 2.0 36.0 90-91 27.180894351370853 33.5 26.0 35.0 2.0 35.0 92-93 26.71397738997668 33.0 25.0 35.0 2.0 35.0 94-95 26.191625315353463 33.0 24.5 35.0 2.0 35.0 96-97 25.574141910549955 33.0 21.5 35.0 2.0 35.0 98-99 24.780974121632504 32.0 10.0 35.0 2.0 35.0 100-101 22.27850854958281 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07659987772098731 1101 2 0.21206597223883605 1101 3 0.32386768595292637 1101 4 0.20409864193220528 1101 5 0.09553725394692236 1101 6 0.24233850521470401 1101 7 0.26481052542257544 1101 8 0.3457205208073333 1101 9 0.41649120070932355 1101 10-11 0.3456132426987537 1101 12-13 0.3482482232464008 1101 14-15 0.4198831631383513 1101 16-17 0.5270558617450902 1101 18-19 0.5490810491627798 1101 20-21 0.6342933516261837 1101 22-23 0.5321955454430878 1101 24-25 0.510008139563638 1101 26-27 0.5273027991934427 1101 28-29 0.6207663615682364 1101 30-31 0.5973297230558217 1101 32-33 0.47467857328641117 1101 34-35 0.612014033754356 1101 36-37 0.5866686256261389 1101 38-39 0.5009567885172501 1101 40-41 0.7097585114319926 1101 42-43 0.7686561448964611 1101 44-45 0.8408880150452376 1101 46-47 0.5542474066302603 1101 48-49 0.5231156258520713 1101 50-51 0.6452586201732018 1101 52-53 0.6908762589009569 1101 54-55 0.7054594311615645 1101 56-57 0.7227393315029715 1101 58-59 0.6001282394919443 1101 60-61 0.5962372873136843 1101 62-63 0.6208536991377187 1101 64-65 0.6860743463510559 1101 66-67 0.627713403372546 1101 68-69 0.6939797249200694 1101 70-71 0.6081217813810191 1101 72-73 0.6156720533470157 1101 74-75 0.5446630037537084 1101 76-77 0.5209405780507517 1101 78-79 0.3884502826924745 1101 80-81 0.3000761685417537 1101 82-83 0.30386473728944097 1101 84-85 0.3618947429256849 1101 86-87 0.18149503309402348 1101 88-89 0.27301707032082945 1101 90-91 0.2746234085206858 1101 92-93 0.17673447066411896 1101 94-95 0.22990980381159432 1101 96-97 0.12164020532183883 1101 98-99 0.09556145248120274 1101 100-101 0.41530111790018154 1102 1 0.1441687887743015 1102 2 0.0770452581031087 1102 3 0.06748649561585296 1102 4 0.2365967230066559 1102 5 0.2875616881181742 1102 6 0.2436991335502725 1102 7 0.20738082956542314 1102 8 0.22720619285937005 1102 9 0.2686729027480794 1102 10-11 0.22536944937697712 1102 12-13 0.23190229443778776 1102 14-15 0.38502487881965664 1102 16-17 0.4344262429456833 1102 18-19 0.49772124341340884 1102 20-21 0.4704806610493222 1102 22-23 0.3658884727761773 1102 24-25 0.33950888457907524 1102 26-27 0.40126990069864377 1102 28-29 0.47596341695729194 1102 30-31 0.5179037183994524 1102 32-33 0.5489529328499856 1102 34-35 0.5686806791929868 1102 36-37 0.525390641553173 1102 38-39 0.515439745327221 1102 40-41 0.5627916786382912 1102 42-43 0.5441388482083767 1102 44-45 0.4646850269522176 1102 46-47 0.594096931732679 1102 48-49 0.4273734912355778 1102 50-51 0.4664934352761705 1102 52-53 0.4587591502224626 1102 54-55 0.33286755226323095 1102 56-57 0.2575395040868713 1102 58-59 0.3001268950499849 1102 60-61 0.2508613362315515 1102 62-63 0.2164099558079684 1102 64-65 0.26728071943052356 1102 66-67 0.10342247709979091 1102 68-69 0.25696360240743843 1102 70-71 0.3139830441693583 1102 72-73 0.14443086098974334 1102 74-75 -0.0017300618976925364 1102 76-77 0.025857564951113687 1102 78-79 -0.022199785069165756 1102 80-81 -0.08117626051991778 1102 82-83 -0.06014615233664955 1102 84-85 -0.09279513057824573 1102 86-87 -0.12921903718102 1102 88-89 -0.12349734006264868 1102 90-91 -0.2711213619096604 1102 92-93 -0.39389981228748994 1102 94-95 -0.35171281557529355 1102 96-97 -0.5190898319705077 1102 98-99 -0.6641243231282488 1102 100-101 -0.2471319717884235 1103 1 0.015070661480866221 1103 2 0.1582702148705799 1103 3 0.2713471076197358 1103 4 0.2259132800103245 1103 5 0.25840477513154525 1103 6 0.17962826383834596 1103 7 0.12837760294262068 1103 8 0.17391551210523204 1103 9 0.19795055047679 1103 10-11 0.16195893016487162 1103 12-13 0.25560123260473233 1103 14-15 0.20680049672846934 1103 16-17 0.3395971432367162 1103 18-19 0.39003692522200595 1103 20-21 0.41118026352095427 1103 22-23 0.42718336606564833 1103 24-25 0.38220759603886023 1103 26-27 0.35709680187540727 1103 28-29 0.42853272080815685 1103 30-31 0.5314444624207155 1103 32-33 0.56259450883379 1103 34-35 0.5710431786657679 1103 36-37 0.5980081074943726 1103 38-39 0.6587719187634704 1103 40-41 0.5692271303654834 1103 42-43 0.7202615758645408 1103 44-45 0.80450323944536 1103 46-47 0.8247518254439257 1103 48-49 0.688581919469577 1103 50-51 0.7667175063040546 1103 52-53 0.7228826107226922 1103 54-55 0.7896442509852406 1103 56-57 0.7648621452779807 1103 58-59 0.7353669410240897 1103 60-61 0.5332098114941175 1103 62-63 0.494367297956515 1103 64-65 0.4879694610476264 1103 66-67 0.5264712138664223 1103 68-69 0.5917334946424546 1103 70-71 0.6656050547982311 1103 72-73 0.5412425938759888 1103 74-75 0.43114300318308096 1103 76-77 0.48060642087080296 1103 78-79 0.49227876210700927 1103 80-81 0.5123657488436173 1103 82-83 0.567689400617926 1103 84-85 0.7268135886844789 1103 86-87 0.47992161088511764 1103 88-89 0.5464538124532758 1103 90-91 0.4466251953049003 1103 92-93 0.44119485113146695 1103 94-95 0.3396763984362643 1103 96-97 0.3867862330259122 1103 98-99 0.5610059016808968 1103 100-101 0.71726880956043 1104 1 0.040407555769373715 1104 2 0.17956437769700173 1104 3 0.01192715928849708 1104 4 0.2340292903440755 1104 5 0.22850060671931516 1104 6 0.2946863911948583 1104 7 0.3087207812696704 1104 8 0.23984118589380898 1104 9 0.29186637927165293 1104 10-11 0.25934756303737316 1104 12-13 0.3029248431339866 1104 14-15 0.4342665454646806 1104 16-17 0.3962638971816972 1104 18-19 0.5101720320506615 1104 20-21 0.5629454674030967 1104 22-23 0.40086328158023576 1104 24-25 0.3809691167761855 1104 26-27 0.4536126516170569 1104 28-29 0.385640230234209 1104 30-31 0.45728757092511074 1104 32-33 0.42368714603529867 1104 34-35 0.5118318255027958 1104 36-37 0.6299537156496626 1104 38-39 0.6141884108735098 1104 40-41 0.5727370277261841 1104 42-43 0.4956674200203821 1104 44-45 0.5340870964485731 1104 46-47 0.6053328371741316 1104 48-49 0.6142727273176476 1104 50-51 0.6022345430307681 1104 52-53 0.6882099916750803 1104 54-55 0.550607279891814 1104 56-57 0.5869802153799171 1104 58-59 0.48438506000188397 1104 60-61 0.4542403938150201 1104 62-63 0.46949952460084177 1104 64-65 0.5848942096860839 1104 66-67 0.44386229053169046 1104 68-69 0.4510657282690431 1104 70-71 0.5788809590015553 1104 72-73 0.3447446361885156 1104 74-75 0.2470922421161923 1104 76-77 0.2624276574299458 1104 78-79 0.08811951409695595 1104 80-81 0.22659036831016977 1104 82-83 0.17289249725247302 1104 84-85 0.23029206585247408 1104 86-87 0.2554134241793484 1104 88-89 0.3624174523080441 1104 90-91 0.32932445169367597 1104 92-93 0.3654342206329311 1104 94-95 0.48326536994862224 1104 96-97 0.29320948047738327 1104 98-99 0.5389541630968999 1104 100-101 0.9529180729314675 1105 1 -0.03954690698940766 1105 2 0.033984739157247645 1105 3 0.055568526648269057 1105 4 0.09610425720280347 1105 5 0.09079276539779357 1105 6 0.12343194262449231 1105 7 0.0918247353025805 1105 8 0.005546035055893128 1105 9 0.06919959618726068 1105 10-11 0.17588201442863038 1105 12-13 0.12143677273260778 1105 14-15 0.2784190314168953 1105 16-17 0.2432219523788106 1105 18-19 0.17474562834253504 1105 20-21 0.2713037843721793 1105 22-23 0.24501933562380884 1105 24-25 0.20081803119488484 1105 26-27 0.20286452780344888 1105 28-29 0.27285261058771226 1105 30-31 0.3045227618767612 1105 32-33 0.36286558670381197 1105 34-35 0.19809370829645445 1105 36-37 0.27397313278304836 1105 38-39 0.28656202278103393 1105 40-41 0.3165208248974025 1105 42-43 0.23556539779411168 1105 44-45 0.3729496560881742 1105 46-47 0.3754899087533943 1105 48-49 0.38954127866416144 1105 50-51 0.4181978479397017 1105 52-53 0.5230654168110647 1105 54-55 0.41676456303791554 1105 56-57 0.2987167592678617 1105 58-59 0.3935243272250588 1105 60-61 0.352591431877741 1105 62-63 0.45746044854340795 1105 64-65 0.5196372609331164 1105 66-67 0.4614662038463777 1105 68-69 0.49302148729442763 1105 70-71 0.40425676297649105 1105 72-73 0.35994159902915257 1105 74-75 0.40462687569000266 1105 76-77 0.4519971189335976 1105 78-79 0.46272053851697237 1105 80-81 0.56631531464188 1105 82-83 0.5035483208393181 1105 84-85 0.3986921672702266 1105 86-87 0.3278534865411906 1105 88-89 0.50056769498023 1105 90-91 0.48676887552247905 1105 92-93 0.537091107930781 1105 94-95 0.7846744302141069 1105 96-97 0.749337824303705 1105 98-99 0.6026856898286219 1105 100-101 0.9425279588113149 1106 1 0.18130226814862027 1106 2 0.18827442293921237 1106 3 0.3111985356481881 1106 4 0.29566235695010334 1106 5 0.2170248679805411 1106 6 0.15731090759140187 1106 7 0.22082683463073494 1106 8 0.15932810644741124 1106 9 0.0902962772509639 1106 10-11 0.16625251761124815 1106 12-13 0.1241376052495724 1106 14-15 0.37068627481392724 1106 16-17 0.4049140040175203 1106 18-19 0.29948268185989946 1106 20-21 0.36605975665229806 1106 22-23 0.4022334217207444 1106 24-25 0.21155174818090927 1106 26-27 0.21771606668509236 1106 28-29 0.14409154553531067 1106 30-31 0.016777985795847883 1106 32-33 0.16146698399261084 1106 34-35 0.24038875612137645 1106 36-37 0.40765069314893765 1106 38-39 0.5028745141916104 1106 40-41 0.6900046389173653 1106 42-43 0.6186357003147762 1106 44-45 0.7157197785125291 1106 46-47 0.6292593796058412 1106 48-49 0.6935228368285422 1106 50-51 0.6218357394581986 1106 52-53 0.47409938734212176 1106 54-55 0.3669522408858725 1106 56-57 0.6189188591836015 1106 58-59 0.5934596678525637 1106 60-61 0.625517190546077 1106 62-63 0.5141491231723521 1106 64-65 0.5787675348381427 1106 66-67 0.580256439473203 1106 68-69 0.5312990115561718 1106 70-71 0.5873957237453986 1106 72-73 0.49735668004637645 1106 74-75 0.4464637666081792 1106 76-77 0.40699491383925945 1106 78-79 0.5260296783800165 1106 80-81 0.49144146693016566 1106 82-83 0.5607275922343646 1106 84-85 0.632894078177884 1106 86-87 0.6239934941994463 1106 88-89 0.8087544382338017 1106 90-91 0.8922561001754019 1106 92-93 1.0054087906981692 1106 94-95 1.085961929467377 1106 96-97 1.1413796453623384 1106 98-99 1.1428412983252976 1106 100-101 1.6454755954104598 1107 1 0.1221292116036814 1107 2 0.03588407175559638 1107 3 0.09920871179403434 1107 4 0.20549529021537438 1107 5 0.12345459600033593 1107 6 0.046934527324076214 1107 7 0.15291773104857498 1107 8 0.13248590592248632 1107 9 0.15336630113156957 1107 10-11 0.14854308790889803 1107 12-13 0.16375827605762083 1107 14-15 0.2741115523134283 1107 16-17 0.19451769616863146 1107 18-19 0.30387532036917975 1107 20-21 0.29323715973341535 1107 22-23 0.21853718922785248 1107 24-25 0.1363639999797499 1107 26-27 0.2099622227659026 1107 28-29 0.24726885340596283 1107 30-31 0.1373043940251577 1107 32-33 0.18952843893190163 1107 34-35 0.21920962820369283 1107 36-37 0.3797877828869005 1107 38-39 0.20515587702046645 1107 40-41 0.2814091979060933 1107 42-43 0.21176887342591755 1107 44-45 0.4871322614160789 1107 46-47 0.4780086293766672 1107 48-49 0.44316295429160135 1107 50-51 0.41598995538057437 1107 52-53 0.33681358244167825 1107 54-55 0.32004439551155883 1107 56-57 0.5099887924030568 1107 58-59 0.516521777566858 1107 60-61 0.5881338842865205 1107 62-63 0.5423150496562243 1107 64-65 0.5245268593703045 1107 66-67 0.211650650417333 1107 68-69 0.4845754130427551 1107 70-71 0.6041850158507636 1107 72-73 0.6265766074329662 1107 74-75 0.73969329562836 1107 76-77 0.39586747577723713 1107 78-79 0.6194302450284788 1107 80-81 0.4665714308314257 1107 82-83 0.34957963355635613 1107 84-85 0.5892551929266325 1107 86-87 0.5729232376543756 1107 88-89 0.7412327567326109 1107 90-91 0.8871049056415856 1107 92-93 0.8882212475406988 1107 94-95 0.9508027622761865 1107 96-97 0.9525205607410356 1107 98-99 1.2629849962540334 1107 100-101 1.4873374001913255 1108 1 0.12975473449183283 1108 2 0.16057848748410564 1108 3 0.20335794518505423 1108 4 0.0827327581879942 1108 5 0.2594659917356523 1108 6 0.1550933223501616 1108 7 0.06286737257535435 1108 8 0.10760442430071038 1108 9 0.18075350550733305 1108 10-11 0.3009888852690139 1108 12-13 0.30608538105545335 1108 14-15 0.34207157386102693 1108 16-17 0.4312249400277679 1108 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2316 100-101 -1.9823657455345334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22021.0 3 5972.0 4 5617.0 5 5868.0 6 6666.0 7 7505.0 8 9340.0 9 10566.0 10 12542.0 11 14600.0 12 17139.0 13 19622.0 14 21448.0 15 22827.0 16 26083.0 17 30890.0 18 28843.0 19 23488.0 20 23775.0 21 25640.0 22 27835.0 23 31159.0 24 35091.0 25 39591.0 26 45655.0 27 53053.0 28 61727.0 29 73128.0 30 85993.0 31 104730.0 32 128175.0 33 162758.0 34 215495.0 35 300840.0 36 435712.0 37 614037.0 38 516065.0 39 81853.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48478338029424 18.66726709647633 14.487163271158348 23.36078625207108 2 15.545524846465336 19.680663428656597 40.543307065447095 24.230504659430977 3 19.448484551520227 23.975469346243617 29.439473486075272 27.13657261616088 4 13.145316943876445 16.25833406511219 36.61528683042982 33.98106216058155 5 14.202633006586252 36.59980780755648 34.089738627243804 15.107820558613463 6 32.516981071192326 35.315518969142765 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22.424855376344567 26.28113719855925 28.837084918367918 22.456922506728265 30-31 22.61288288489009 26.07451951868655 28.6942718161043 22.61832578031906 32-33 22.363063711196308 26.169010583477537 28.702364790577167 22.76556091474899 34-35 22.902694078999545 26.26284859583069 28.460026939892195 22.374430385277567 36-37 22.89850295461802 26.010652701368254 28.459534598826234 22.63130974518749 38-39 22.600640925581185 26.220779452068943 28.561491276656533 22.617088345693343 40-41 22.82744768562485 26.15304359586117 28.538853772278006 22.48065494623598 42-43 22.638065473126183 26.22367031656678 28.341044375857503 22.797219834449535 44-45 22.68048350037895 26.37401056751608 28.26135564971785 22.684150282387115 46-47 22.845108350755186 26.507678786529016 27.879380308875685 22.767832553840112 48-49 22.891191286332706 26.589526754679 27.690576666077337 22.828705292910954 50-51 22.775607338401976 26.84782197044826 27.611651305105262 22.7649193860445 52-53 23.053329485278795 26.63928021421369 27.57411496741528 22.73327533309223 54-55 22.780206366419765 26.606165247308155 27.737939943135853 22.875688443136223 56-57 22.797472624135295 26.870129143267945 27.521756580697858 22.810641651898898 58-59 22.80143046700831 26.577898078851632 27.617818808609435 23.002852645530627 60-61 22.77198165708147 26.61336964307107 27.83586195818627 22.778786741661193 62-63 22.66782307803981 26.633989813801804 27.88785304096779 22.810334067190592 64-65 22.598422096458513 26.62098770108374 27.77645612931401 23.004134073143735 66-67 22.55240554111481 26.73975826083389 27.578459524807318 23.129376673243982 68-69 22.63971182312183 26.58133138963471 27.722560579372317 23.056396207871142 70-71 22.628966969093746 26.487970419592305 27.64459490518054 23.238467706133413 72-73 22.71499057687943 26.602020326632246 27.53085795012543 23.15213114636289 74-75 22.622495751616228 26.846357094190832 27.424640311491753 23.106506842701187 76-77 22.693284376864025 26.642517735618597 27.471120448032522 23.19307743948485 78-79 22.685830598322422 26.639644778797823 27.499489710712368 23.17503491216739 80-81 22.71689379833928 26.742855637703993 27.34869474679529 23.191555817161436 82-83 22.77117407084107 26.712428619495967 27.372993892020116 23.143403417642855 84-85 22.69166718804305 26.686767347688995 27.30834777969997 23.313217684567988 86-87 22.734872596569396 26.619141234498795 27.387595443126262 23.258390725805548 88-89 22.71376646019743 26.530680671907902 27.26220176007527 23.49335110781939 90-91 22.682943334487142 26.63896531032426 27.283161080682273 23.39493027450633 92-93 22.792142861313444 26.457201656009815 27.329282188642175 23.421373294034563 94-95 22.696893236677944 26.314987255287136 27.40436674109068 23.583752766944237 96-97 22.81428985747245 26.12336775928654 27.441123594597155 23.62121878864385 98-99 22.694412353562267 26.140389175061703 27.30799820570545 23.85720026567058 100-101 23.034232593394886 25.622086251011478 27.611495943765036 23.732185211828597 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4243.0 1 2755.0 2 1336.0 3 1468.0 4 1339.5 5 1302.5 6 1883.0 7 3449.0 8 5302.5 9 5806.5 10 4699.0 11 3548.0 12 3399.5 13 3566.5 14 3747.5 15 4056.0 16 4355.0 17 4550.5 18 4765.0 19 5114.0 20 5588.0 21 6450.0 22 7772.5 23 9368.0 24 11564.0 25 14429.0 26 18120.0 27 22495.5 28 26401.0 29 30799.5 30 36176.5 31 42088.5 32 48889.0 33 54231.0 34 58827.5 35 63704.5 36 69916.0 37 76706.0 38 81594.5 39 85392.5 40 88782.0 41 93052.0 42 97265.0 43 102853.5 44 108912.5 45 113650.0 46 116392.0 47 119284.5 48 125029.5 49 127218.5 50 125602.0 51 124130.0 52 121926.5 53 121346.5 54 120544.0 55 114131.0 56 104956.5 57 95206.0 58 87556.5 59 79060.5 60 67353.0 61 56512.5 62 46653.5 63 36557.5 64 27901.0 65 21660.5 66 16403.5 67 12774.5 68 9730.0 69 7184.0 70 5520.0 71 4222.5 72 3339.0 73 2757.5 74 2215.0 75 1801.0 76 1352.0 77 910.0 78 635.0 79 470.0 80 339.0 81 252.5 82 210.0 83 163.5 84 123.0 85 98.0 86 61.5 87 38.0 88 32.0 89 33.5 90 29.0 91 21.0 92 12.0 93 7.0 94 9.5 95 9.5 96 4.5 97 2.0 98 2.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27163637881778985 2 0.1621945619047051 3 0.19037508424336042 4 0.1602562190983426 5 0.2318556202995187 6 0.19138898663438084 7 0.23808813793843825 8 0.243843525040407 9 0.14448109072040746 10-11 0.202452450959927 12-13 0.20820783806189574 14-15 0.16899367205625368 16-17 0.2843996206812231 18-19 0.22778510040615735 20-21 0.19481836236871455 22-23 0.24036941831823413 24-25 0.22809821732103133 26-27 0.33856884695441614 28-29 0.30992610440808976 30-31 0.28542843340152324 32-33 0.1871544531189427 34-35 0.3538817551246802 36-37 0.3906655374577292 38-39 0.37068569622291536 40-41 0.333454604011475 42-43 0.36631696974395983 44-45 0.3750246020433115 46-47 0.38015375531553236 48-49 0.4005361754408985 50-51 0.3927529835568888 52-53 0.39255914927625263 54-55 0.38655028657652873 56-57 0.3640207790348842 58-59 0.3397914939553525 60-61 0.3068993075643082 62-63 0.3752929879703463 64-65 0.4084088293005863 66-67 0.4136572652070449 68-69 0.41874168749142654 70-71 0.4177128747711265 72-73 0.40919907675241096 74-75 0.40497945356625253 76-77 0.38759400962610857 78-79 0.361963153594284 80-81 0.37205744651664885 82-83 0.38265869063452396 84-85 0.38358313104986613 86-87 0.3790951219366729 88-89 0.3880114988459405 90-91 0.4089008701668168 92-93 0.4075142095438035 94-95 0.4161771108553161 96-97 0.41720592357561626 98-99 0.4146860779273449 100-101 0.38668447954004614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3353380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.243143084533926 #Duplication Level Percentage of deduplicated Percentage of total 1 75.08745745000242 41.48067155764337 2 13.800137211195807 15.24725909088582 3 4.741022667431885 7.85726980751875 4 2.113278828379817 4.669766587748098 5 1.1610716652495836 3.207062406739042 6 0.7045883070380793 2.3354203596836487 7 0.4573550052929636 1.768600959847887 8 0.3205178508917089 1.416513079836639 9 0.24127022183483304 1.1995672848173031 >10 1.256565586207514 12.532562875749656 >50 0.08150627184748382 3.0724976292788133 >100 0.032687171441213254 3.2526450137549254 >500 0.0018387223065888048 0.6900649744288221 >1k 5.408006770211329E-4 0.4298789893838444 >5k 1.0816013540422656E-4 0.48222091650876586 >10k+ 5.408006770211328E-5 0.35799846617462 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11983 0.35734095151757334 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9690 0.2889621814408149 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6451 0.19237306836684182 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.9641317118847255E-5 2 0.0 0.0 0.0 0.0 5.9641317118847255E-5 3 0.0 0.0 0.0 0.0 5.9641317118847255E-5 4 0.0 0.0 0.0 0.0 8.946197567827089E-5 5 0.0 0.0 0.0 0.0 8.946197567827089E-5 6 8.946197567827089E-5 0.0 0.0 0.0 1.1928263423769451E-4 7 1.1928263423769451E-4 0.0 0.0 0.0 1.1928263423769451E-4 8 1.1928263423769451E-4 0.0 0.0 0.0 1.7892395135654177E-4 9 1.1928263423769451E-4 0.0 0.0 0.0 1.7892395135654177E-4 10-11 1.1928263423769451E-4 0.0 0.0 0.0 1.7892395135654177E-4 12-13 1.1928263423769451E-4 0.0 0.0 0.0 1.938342806362536E-4 14-15 1.1928263423769451E-4 0.0 0.0 2.9820658559423627E-5 2.6838592703481263E-4 16-17 1.938342806362536E-4 0.0 0.0 4.473098783913544E-5 3.280272441536599E-4 18-19 2.0874460991596538E-4 0.0 0.0 5.9641317118847255E-5 3.280272441536599E-4 20-21 2.0874460991596538E-4 0.0 0.0 7.455164639855907E-5 3.7275823199279536E-4 22-23 2.0874460991596538E-4 0.0 0.0 1.4910329279711813E-4 5.069511955102016E-4 24-25 2.0874460991596538E-4 0.0 0.0 3.876685612725071E-4 5.964131711884725E-4 26-27 2.0874460991596538E-4 0.0 0.0 7.156958054261671E-4 5.964131711884725E-4 28-29 2.38565268475389E-4 0.0 0.0 0.0024602043311524493 5.964131711884725E-4 30-31 2.6838592703481263E-4 0.0 0.0 0.00790247451824726 6.113235004681844E-4 32-33 2.8329625631452445E-4 0.0 0.0 0.019115042136590543 6.262338297478962E-4 34-35 4.02578890552219E-4 0.0 0.0 0.03506909446588218 6.560544883073198E-4 36-37 5.367718540696253E-4 0.0 0.0 0.06409950557348108 6.560544883073198E-4 38-39 5.665925126290489E-4 0.0 0.0 0.10528183504404512 6.560544883073198E-4 40-41 5.665925126290489E-4 0.0 0.0 0.1485665209430485 6.858751468667434E-4 42-43 6.858751468667434E-4 2.9820658559423627E-5 0.0 0.1949078243443928 6.858751468667434E-4 44-45 7.455164639855906E-4 2.9820658559423627E-5 0.0 0.25696461480655336 7.007854761464553E-4 46-47 7.753371225450142E-4 2.9820658559423627E-5 0.0 0.33390191388986634 7.306061347058788E-4 48-49 7.753371225450142E-4 2.9820658559423627E-5 0.0 0.4133441482921709 7.455164639855906E-4 50-51 7.753371225450142E-4 2.9820658559423627E-5 0.0 0.4996600444924225 7.455164639855906E-4 52-53 7.753371225450142E-4 2.9820658559423627E-5 0.0 0.5871389463764918 8.498887689435733E-4 54-55 8.200681103841497E-4 2.9820658559423627E-5 0.0 0.6879476826366233 9.244404153421324E-4 56-57 8.647990982232852E-4 2.9820658559423627E-5 0.0 0.8080951159725411 9.244404153421324E-4 58-59 8.647990982232852E-4 2.9820658559423627E-5 0.0 0.9437791124179187 9.244404153421324E-4 60-61 8.647990982232852E-4 2.9820658559423627E-5 0.0 1.093702473325421 9.542610739015561E-4 62-63 8.647990982232852E-4 2.9820658559423627E-5 0.0 1.2473235958942916 9.542610739015561E-4 64-65 8.647990982232852E-4 2.9820658559423627E-5 0.0 1.4101443916287448 9.542610739015561E-4 66-67 9.244404153421324E-4 2.9820658559423627E-5 0.0 1.5988346086634977 9.989920617406915E-4 68-69 9.840817324609796E-4 2.9820658559423627E-5 0.0 1.8147361766337249 0.0010437230495798269 70-71 9.840817324609796E-4 2.9820658559423627E-5 0.0 2.0474715063607465 0.0010884540374189624 72-73 9.989920617406913E-4 2.9820658559423627E-5 0.0 2.2837256737977802 0.0011630056838175214 74-75 0.0010139023910204032 2.9820658559423627E-5 0.0 2.5294180796688712 0.0011630056838175214 76-77 0.0010139023910204032 2.9820658559423627E-5 0.0 2.80369358676917 0.0011779160130972331 78-79 0.0010139023910204032 2.9820658559423627E-5 0.0 3.1123523131884845 0.001192826342376945 80-81 0.0011182746959783859 2.9820658559423627E-5 0.0 3.4530384269006196 0.0012524676594957923 82-83 0.0011331850252580978 2.9820658559423627E-5 0.0 3.8002105338494294 0.001371750293733487 84-85 0.0012375573302160804 2.9820658559423627E-5 0.0 4.148754391091973 0.001371750293733487 86-87 0.0012524676594957923 2.9820658559423627E-5 0.0 4.516443111129666 0.001371750293733487 88-89 0.0012524676594957923 2.9820658559423627E-5 0.0 4.920020993743625 0.001371750293733487 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6920 0.0 44.708683 1 GTATCAA 11800 0.0 39.791653 1 ATCAACG 14895 0.0 31.312784 3 TCAACGC 15050 0.0 30.958769 4 TATCAAC 15240 0.0 30.696873 2 CAACGCA 15220 0.0 30.488277 5 AACGCAG 15770 0.0 29.54354 6 ACGCAGA 17730 0.0 26.117031 7 CGCAGAG 18015 0.0 25.62523 8 GCAGAGT 19415 0.0 23.361633 9 GTACATG 12295 0.0 22.496319 1 TACATGG 12355 0.0 22.119837 2 GAGTACT 11005 0.0 21.611763 12-13 GTGGTAT 4115 0.0 21.019314 1 ACATGGG 12530 0.0 20.940294 3 AGAGTAC 17035 0.0 20.633678 10-11 AGTACTT 11605 0.0 20.330767 12-13 TGGTATC 4090 0.0 20.068966 2 GTACTTT 11850 0.0 20.030758 14-15 CAGAGTA 18775 0.0 19.74535 10-11 >>END_MODULE