FastQCFastQC Report
Thu 26 May 2016
SRR938690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938690_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3353380
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115350.3439812964829515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105340.31413081726496844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68670.20477846232756203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80650.045.056441
GTATCAA129150.035.1335331
ATCAACG165700.027.1506983
TCAACGC167700.026.8268974
TATCAAC169500.026.7099972
CAACGCA171250.026.2707775
AACGCAG177000.025.4380916
ACGCAGA200700.022.3619357
CGCAGAG204150.021.8910778
GTACATG119700.020.9006351
GAGTACT129900.020.45485112-13
TACATGG120100.020.1521932
GCAGAGT219450.020.0405589
AGAGTAC192950.019.63575410-11
GTGGTAT45400.019.4861011
GTACTTT134600.019.42334414-15
ACATGGG122750.018.9825763
AGTACTT133900.018.81617712-13
TGGTATC46050.018.549752
CAGAGTA213900.018.15621610-11
Produced by FastQC (version 0.11.5)