FastQCFastQC Report
Thu 26 May 2016
SRR938689_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938689_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3735978
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136490.3653394104569138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103530.2771161928683734No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76100.20369498963858998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69920.18715313634073863No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53610.14349656234592387No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38630.10339996648802535No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38020.10176719456056756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG135800.025.805221
GTATCAA208200.025.372741
TACATGG137400.024.6993472
ACATGGG138700.023.340143
GGTATCA154300.022.2810171
ATCAACG242850.021.6252353
GTGTAGC20650.021.5859261
TCAACGC244500.021.5180684
TATCAAC247200.021.378912
CAACGCA246000.021.3483265
GAGTACT120350.021.19460712-13
AACGCAG250850.021.0154046
AGAGTAC189650.020.23102810-11
CATGGGG83700.019.7632874
GTACTTT129100.019.53795614-15
ACGCAGA274150.019.161487
AGTACTT127900.019.0731512-13
CGCAGAG278050.018.8416088
CCTATAC7850.018.113853
CATGGGA60950.017.963774
Produced by FastQC (version 0.11.5)