##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938689_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3735978 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.046808894484926 33.0 31.0 34.0 30.0 34.0 2 32.168025347044335 34.0 31.0 34.0 30.0 34.0 3 32.19190744699246 34.0 31.0 34.0 30.0 34.0 4 35.72572857763081 37.0 35.0 37.0 35.0 37.0 5 35.741676744349135 37.0 35.0 37.0 35.0 37.0 6 35.828263710332344 37.0 35.0 37.0 35.0 37.0 7 35.76390492663501 37.0 35.0 37.0 35.0 37.0 8 35.74403596595055 37.0 35.0 37.0 35.0 37.0 9 37.40550238786203 39.0 37.0 39.0 35.0 39.0 10-11 37.31485905430921 39.0 37.0 39.0 34.5 39.0 12-13 37.21630721594185 39.0 37.0 39.0 34.0 39.0 14-15 38.48868502437648 40.0 38.0 41.0 34.0 41.0 16-17 38.41570988908393 40.0 38.0 41.0 34.0 41.0 18-19 38.34264227466008 40.0 38.0 41.0 34.0 41.0 20-21 38.234349078072725 40.0 38.0 41.0 34.0 41.0 22-23 38.1638437110711 40.0 38.0 41.0 34.0 41.0 24-25 38.188246424363314 40.0 38.0 41.0 34.0 41.0 26-27 38.103792367085674 40.0 38.0 41.0 34.0 41.0 28-29 37.99002429885829 40.0 38.0 41.0 33.0 41.0 30-31 37.80934577237875 40.0 38.0 41.0 33.0 41.0 32-33 37.617939800502036 40.0 37.0 41.0 33.0 41.0 34-35 37.31849786053344 40.0 37.0 41.0 32.0 41.0 36-37 37.08778745485118 40.0 37.0 41.0 31.0 41.0 38-39 36.92317834848064 40.0 36.0 41.0 31.0 41.0 40-41 36.751963876660945 39.5 36.0 41.0 31.0 41.0 42-43 36.500032923106076 39.0 36.0 41.0 30.0 41.0 44-45 36.50639752161281 39.5 36.0 41.0 30.0 41.0 46-47 36.35195362499458 39.5 36.0 41.0 30.0 41.0 48-49 36.13959222993283 39.0 36.0 41.0 29.5 41.0 50-51 35.021393193428864 38.0 34.0 40.0 27.5 40.5 52-53 35.12972734314817 38.5 34.5 39.5 28.0 40.5 54-55 35.64563214772678 39.0 35.0 40.5 28.0 41.0 56-57 35.5393408365895 39.0 35.0 41.0 28.0 41.0 58-59 35.308241108486186 38.5 35.0 40.5 28.0 41.0 60-61 35.014895028825116 38.0 35.0 40.0 27.0 41.0 62-63 34.660556486146334 38.0 34.0 40.0 26.0 41.0 64-65 34.27092785342954 37.0 34.0 40.0 26.0 41.0 66-67 33.872907308340686 36.5 34.0 39.5 25.5 41.0 68-69 33.46960675357296 36.0 33.0 39.0 24.5 41.0 70-71 33.06807722101147 35.5 33.0 39.0 23.5 40.0 72-73 32.66176032085842 35.0 33.0 38.0 22.5 40.0 74-75 32.222347401403326 35.0 32.0 37.0 21.5 39.5 76-77 31.802789122419888 35.0 32.0 37.0 20.0 39.0 78-79 31.399783269601695 35.0 31.5 36.0 19.5 39.0 80-81 30.985893118214292 35.0 31.0 36.0 18.0 37.5 82-83 30.55813297080443 34.0 31.0 35.0 14.5 37.0 84-85 30.188905020318643 34.0 31.0 35.0 8.5 36.5 86-87 29.846724070644953 34.0 30.0 35.0 6.0 36.0 88-89 29.51344574298885 34.0 30.0 35.0 2.0 36.0 90-91 29.14700314081079 34.0 29.5 35.0 2.0 35.5 92-93 28.743862383557932 34.0 29.0 35.0 2.0 35.0 94-95 28.291265633791205 34.0 29.0 35.0 2.0 35.0 96-97 27.768915261278305 34.0 28.5 35.0 2.0 35.0 98-99 27.13051709619275 34.0 27.0 35.0 2.0 35.0 100-101 24.910523295372723 31.0 21.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20626008816790176 1101 2 0.08461197050613123 1101 3 0.22371652875557402 1101 4 0.10603319717484538 1101 5 0.03486693379885253 1101 6 0.06646059861772358 1101 7 0.08360940289496455 1101 8 0.18248833026765965 1101 9 0.20119390676159554 1101 10-11 0.18226410311802965 1101 12-13 0.15592722579348361 1101 14-15 0.2923992870701966 1101 16-17 0.3280425756077392 1101 18-19 0.27363005642493476 1101 20-21 0.30208218314369617 1101 22-23 0.2908780444782053 1101 24-25 0.27367283871794257 1101 26-27 0.30332095444761364 1101 28-29 0.1787415975206983 1101 30-31 0.15743165279399562 1101 32-33 0.18428451819339386 1101 34-35 0.23675276843793114 1101 36-37 0.25779679447339277 1101 38-39 0.2331641785828822 1101 40-41 0.22680845392586235 1101 42-43 0.16241544930301188 1101 44-45 0.2958410344378066 1101 46-47 0.3145440408110929 1101 48-49 0.27425606176950623 1101 50-51 0.33895643470281556 1101 52-53 0.3357834475377288 1101 54-55 0.2831831220495431 1101 56-57 0.23877095224649736 1101 58-59 0.1854971478752887 1101 60-61 0.10953917321199924 1101 62-63 0.08595473763595862 1101 64-65 0.15403670006106296 1101 66-67 0.0699258390946298 1101 68-69 0.07997011038845159 1101 70-71 0.0642799105885743 1101 72-73 -0.1419360502235918 1101 74-75 -0.05398450569406066 1101 76-77 -0.15709017348919474 1101 78-79 -0.17958572327422218 1101 80-81 -0.3586062226904332 1101 82-83 -0.49833212866430543 1101 84-85 -0.41693386225507467 1101 86-87 -0.3089356277678128 1101 88-89 -0.37023015789189984 1101 90-91 -0.3888047252118163 1101 92-93 -0.4675698915434232 1101 94-95 -0.5605459698528428 1101 96-97 -0.6630763205606236 1101 98-99 -0.5404751403067323 1101 100-101 -0.5324872786914732 1102 1 0.2584275933366911 1102 2 0.23371262290668682 1102 3 0.2301417158062975 1102 4 0.19187750604259435 1102 5 0.17419328528079348 1102 6 0.16325054500570957 1102 7 0.2596153441756073 1102 8 0.19255293534487095 1102 9 0.3198897459205199 1102 10-11 0.2753891662024657 1102 12-13 0.28257196851018307 1102 14-15 0.16747391356072683 1102 16-17 0.2773768272784949 1102 18-19 0.31395604239747144 1102 20-21 0.3620172592544577 1102 22-23 0.33342490025729177 1102 24-25 0.3153932635766594 1102 26-27 0.29529873073495594 1102 28-29 0.28196394060928043 1102 30-31 0.34403247220708266 1102 32-33 0.36132845062481067 1102 34-35 0.23255810815597044 1102 36-37 0.2781059151567149 1102 38-39 0.34551360769106054 1102 40-41 0.24616940643570473 1102 42-43 0.28319623321817033 1102 44-45 0.36689153863044766 1102 46-47 0.34450681652894133 1102 48-49 0.5029989713434375 1102 50-51 0.4344758952675605 1102 52-53 0.2688885458449377 1102 54-55 0.21398473722277345 1102 56-57 0.2505947118923828 1102 58-59 0.28232770641943006 1102 60-61 0.3130179483334956 1102 62-63 0.2639614890431261 1102 64-65 0.24602766537761767 1102 66-67 0.2613537505992838 1102 68-69 0.14195854769749872 1102 70-71 0.13882038139590236 1102 72-73 0.14116847924885434 1102 74-75 0.08135259438368792 1102 76-77 0.18618189369468396 1102 78-79 -0.07262914534181064 1102 80-81 -0.12734783981886721 1102 82-83 -0.14922937289800942 1102 84-85 -0.23787866901198385 1102 86-87 -0.2290275395592687 1102 88-89 -0.4112507837080308 1102 90-91 -0.32876944158220667 1102 92-93 -0.33219075722261593 1102 94-95 -0.4683214184948028 1102 96-97 -0.34608276572303254 1102 98-99 -0.35700162227672294 1102 100-101 -0.39467964987652593 1103 1 0.11944675369130664 1103 2 0.19446348621301723 1103 3 0.2132609815938551 1103 4 0.2773824215527796 1103 5 0.15322544255963066 1103 6 0.2413027941810384 1103 7 0.11587223026661775 1103 8 0.18469188285363458 1103 9 0.2817444379779488 1103 10-11 0.2274458374517465 1103 12-13 0.2829413581951954 1103 14-15 0.362714998891164 1103 16-17 0.3636406002059829 1103 18-19 0.4632163452685205 1103 20-21 0.4646866190742003 1103 22-23 0.4655612131021627 1103 24-25 0.3255643517319413 1103 26-27 0.32436886070354376 1103 28-29 0.34211021614144954 1103 30-31 0.3533812881224705 1103 32-33 0.19888783741342309 1103 34-35 0.292321542374097 1103 36-37 0.36768495139586577 1103 38-39 0.26690145930620446 1103 40-41 0.3559528226180362 1103 42-43 0.27781153948890136 1103 44-45 0.29675090151756933 1103 46-47 0.30310575051053235 1103 48-49 0.21273630741330152 1103 50-51 0.3168078696771275 1103 52-53 0.31187325955948353 1103 54-55 0.1849502242522547 1103 56-57 0.26842832504831193 1103 58-59 0.2743086138530728 1103 60-61 0.26903191655809877 1103 62-63 0.24736867270338792 1103 64-65 0.12132347697105672 1103 66-67 0.17256892371876376 1103 68-69 0.17669187975414502 1103 70-71 0.18734464823408103 1103 72-73 0.2518102153863424 1103 74-75 0.10351799654200988 1103 76-77 0.14768644581209145 1103 78-79 0.19174221397976154 1103 80-81 0.07700649225838418 1103 82-83 0.18618050320850799 1103 84-85 0.19372589364805748 1103 86-87 0.227301349192377 1103 88-89 0.11945086982849773 1103 90-91 0.09078557587873703 1103 92-93 -0.06139710647103769 1103 94-95 -0.06418736843923512 1103 96-97 0.028690188338860168 1103 98-99 0.007848899974494117 1103 100-101 0.06402053577583544 1104 1 0.11891854521410039 1104 2 0.06412500524880471 1104 3 0.12043377133095845 1104 4 0.1326610163515909 1104 5 0.1052881670089576 1104 6 0.05057978509467631 1104 7 0.012496353863305387 1104 8 0.006171828210618457 1104 9 0.03872749200642289 1104 10-11 0.08519637143130865 1104 12-13 0.1536773245756251 1104 14-15 0.13859168222273865 1104 16-17 0.13679776797589227 1104 18-19 0.013996241601965664 1104 20-21 0.05000823547682387 1104 22-23 0.10281069483089311 1104 24-25 0.10657300317759422 1104 26-27 0.027183910014606738 1104 28-29 0.08431535062289441 1104 30-31 0.027872165223094214 1104 32-33 -0.06285052714549977 1104 34-35 -0.03749950736889218 1104 36-37 0.013379246402827505 1104 38-39 -0.035936223608345585 1104 40-41 -0.1142984677500607 1104 42-43 6.684602834425846E-4 1104 44-45 0.1430121840001064 1104 46-47 0.1961579786774692 1104 48-49 0.16896432519180848 1104 50-51 0.1853903654737863 1104 52-53 0.25769449807518896 1104 54-55 0.11254430908223867 1104 56-57 0.11841920057810995 1104 58-59 0.18254977250780513 1104 60-61 0.1901955719902375 1104 62-63 0.13226453968718488 1104 64-65 0.0894905156966459 1104 66-67 0.03385563383229595 1104 68-69 0.13475233838444467 1104 70-71 0.007993180881314288 1104 72-73 -0.02986993150123851 1104 74-75 -0.061780318313140015 1104 76-77 -0.1848189124237578 1104 78-79 -0.1013253878267193 1104 80-81 -0.0626608033319691 1104 82-83 -0.13072003036810642 1104 84-85 -0.0820275137200639 1104 86-87 -5.926752767742016E-4 1104 88-89 0.013893555247499023 1104 90-91 0.03870704751918552 1104 92-93 0.011380378161124582 1104 94-95 0.015345912177970433 1104 96-97 0.021680293615848 1104 98-99 0.03853870719001051 1104 100-101 0.11418258390796154 1105 1 0.139130768074466 1105 2 0.08916669237754604 1105 3 0.0858796502010648 1105 4 0.19010116306808555 1105 5 0.1480176820510053 1105 6 0.20207913447026726 1105 7 0.15985889731918945 1105 8 0.08510824458804223 1105 9 0.07683299581722025 1105 10-11 0.20264871215318436 1105 12-13 0.20092264465785803 1105 14-15 0.27131739617551176 1105 16-17 0.17120636565140046 1105 18-19 0.20411895852070217 1105 20-21 0.14579859846698184 1105 22-23 0.24823530702267504 1105 24-25 0.233667555966953 1105 26-27 0.21917477907679483 1105 28-29 0.27730019065807454 1105 30-31 0.19993138452930026 1105 32-33 0.3039784488077544 1105 34-35 0.22143464777659716 1105 36-37 0.23921409878460764 1105 38-39 0.25871783564980433 1105 40-41 0.267278040963312 1105 42-43 0.21981790413136792 1105 44-45 0.3411561010087283 1105 46-47 0.3349837472882413 1105 48-49 0.249425893959625 1105 50-51 0.3018676019431652 1105 52-53 0.36270257192175137 1105 54-55 0.3586305050242231 1105 56-57 0.2906192960215179 1105 58-59 0.38377819022809234 1105 60-61 0.34101363853982036 1105 62-63 0.24659700459647382 1105 64-65 0.30495581619079104 1105 66-67 0.24326631239609497 1105 68-69 0.12109942222758718 1105 70-71 0.13356958693752574 1105 72-73 0.09956305418502609 1105 74-75 0.060793990207685056 1105 76-77 0.0898097183794917 1105 78-79 -0.0192930258520434 1105 80-81 0.09792953080319933 1105 82-83 0.23916611729727677 1105 84-85 0.11459693865950271 1105 86-87 0.08407298146544306 1105 88-89 0.14480917384009828 1105 90-91 0.2572350687608953 1105 92-93 0.26310370047962195 1105 94-95 0.2567391501233871 1105 96-97 0.16735253151409424 1105 98-99 0.30022990059331534 1105 100-101 0.5886794251195404 1106 1 0.11901703498579508 1106 2 0.11753323885241684 1106 3 0.11705509172411865 1106 4 0.10685337549531937 1106 5 0.1318016276781222 1106 6 0.05404925333610322 1106 7 0.15985740026837902 1106 8 0.14033658760943268 1106 9 0.10894003067284075 1106 10-11 0.11680664245905348 1106 12-13 0.14967089631637975 1106 14-15 0.16910454121406815 1106 16-17 0.16192293971478477 1106 18-19 0.22993517200870173 1106 20-21 0.16683515644776037 1106 22-23 0.2175698251647944 1106 24-25 0.11709413417930392 1106 26-27 0.026860744867043707 1106 28-29 0.11733100851832035 1106 30-31 0.08836431451437221 1106 32-33 0.15148200965003866 1106 34-35 0.10183921506475713 1106 36-37 0.18949351262506298 1106 38-39 0.12082833510872604 1106 40-41 0.1406748064781027 1106 42-43 0.21951421668135396 1106 44-45 0.17550582490978428 1106 46-47 0.3453957356711257 1106 48-49 0.4251574171446606 1106 50-51 0.3534182257856173 1106 52-53 0.21543415498882013 1106 54-55 0.24616295894713858 1106 56-57 0.29031186594448144 1106 58-59 0.2997472312173457 1106 60-61 0.3944453067249185 1106 62-63 0.2104954104512302 1106 64-65 0.2236129188414253 1106 66-67 0.20284412268126317 1106 68-69 0.39755904676724185 1106 70-71 0.39363458032912035 1106 72-73 0.31731283711265235 1106 74-75 0.18361161092344958 1106 76-77 0.08732044367922498 1106 78-79 0.3928927538761826 1106 80-81 0.13391884613770344 1106 82-83 0.21256363133747413 1106 84-85 0.31336903619877887 1106 86-87 0.4714992517130554 1106 88-89 0.42863182892386575 1106 90-91 0.37305643638096697 1106 92-93 0.4942093152756186 1106 94-95 0.6016278978617677 1106 96-97 0.6848995709891845 1106 98-99 0.7109264355622855 1106 100-101 0.8410209195184315 1107 1 0.13355487351643802 1107 2 0.15265417243242752 1107 3 0.031276876648639984 1107 4 0.18539239753094705 1107 5 0.05023950322247828 1107 6 0.04725248865824483 1107 7 -0.029768927582054516 1107 8 0.08050189022532805 1107 9 0.032251621187114665 1107 10-11 0.11593287379955797 1107 12-13 0.10032751398692596 1107 14-15 0.19187669009701835 1107 16-17 0.19351724873651932 1107 18-19 0.2895596703284937 1107 20-21 0.2646369549609915 1107 22-23 0.23457129302027369 1107 24-25 0.22257263011837125 1107 26-27 0.15710093130992675 1107 28-29 0.017927276480065757 1107 30-31 0.13877587137053382 1107 32-33 0.17935164085663757 1107 34-35 0.04499143799554162 1107 36-37 0.1840987680466455 1107 38-39 0.241039804923588 1107 40-41 0.2120105257878393 1107 42-43 0.2545474670146888 1107 44-45 0.25964074506186563 1107 46-47 0.2337735766411484 1107 48-49 0.24850003964990464 1107 50-51 0.3479359438561076 1107 52-53 0.21631310294159078 1107 54-55 0.2845696799482411 1107 56-57 0.2928512150780094 1107 58-59 0.27573912788475496 1107 60-61 0.11541671746374504 1107 62-63 0.17440128037043223 1107 64-65 0.18348284271456805 1107 66-67 0.2317176978832194 1107 68-69 0.25656383899953283 1107 70-71 0.34366038196335325 1107 72-73 0.23597294810709712 1107 74-75 0.3049806163109352 1107 76-77 0.2507407147327996 1107 78-79 0.346179941894885 1107 80-81 0.2869663787734531 1107 82-83 0.4453521103132623 1107 84-85 0.4159250611976937 1107 86-87 0.45932915263390584 1107 88-89 0.4795877222082474 1107 90-91 0.6396920345827759 1107 92-93 0.7304613919169043 1107 94-95 0.738863959982357 1107 96-97 0.6742323423327257 1107 98-99 0.7500512181596264 1107 100-101 1.0541788192464345 1108 1 0.06592514428201923 1108 2 0.053092592899865565 1108 3 0.006497917365074102 1108 4 0.013076531455560314 1108 5 0.01660288501871321 1108 6 0.03343317455997408 1108 7 0.06551423256907896 1108 8 0.10366980809492787 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58-59 -1.0815583301783036 2316 60-61 -0.9362796258320927 2316 62-63 -1.1970145668857484 2316 64-65 -1.3362328257651868 2316 66-67 -1.3271019192423452 2316 68-69 -1.4099281043398122 2316 70-71 -1.2249473148446484 2316 72-73 -1.2127536129891396 2316 74-75 -1.2981819414456908 2316 76-77 -1.381618381803058 2316 78-79 -1.2931782390219908 2316 80-81 -1.1913004677033143 2316 82-83 -1.122563159840329 2316 84-85 -1.0545609157066274 2316 86-87 -1.3150465134748224 2316 88-89 -1.2676213536219443 2316 90-91 -1.5532728111975196 2316 92-93 -1.5212608278901136 2316 94-95 -1.4605744648265677 2316 96-97 -1.5205812866951405 2316 98-99 -1.6491291685725464 2316 100-101 -1.8744510476779688 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12111.0 3 3145.0 4 3050.0 5 3367.0 6 3543.0 7 4256.0 8 4919.0 9 6005.0 10 7344.0 11 9382.0 12 12316.0 13 16653.0 14 19244.0 15 17325.0 16 21513.0 17 27385.0 18 23927.0 19 17340.0 20 17377.0 21 18261.0 22 19483.0 23 21586.0 24 24580.0 25 28794.0 26 33564.0 27 40053.0 28 48647.0 29 59339.0 30 73941.0 31 94089.0 32 122922.0 33 166520.0 34 236494.0 35 353775.0 36 542242.0 37 802490.0 38 703920.0 39 114982.0 40 94.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39332086727725 18.529355632134116 14.303566335644271 23.773757164944364 2 15.451282106233192 19.694367947879943 40.389052002185956 24.465297943700907 3 19.61869984425904 23.605175507256394 29.19106313696686 27.585061511517704 4 13.017951064568537 16.119790215485647 36.5787099152546 34.283548804691215 5 14.403708345358016 36.494585743964144 33.79154410868224 15.3101618019956 6 32.640995000844754 35.00527868596999 17.40657073907762 14.947155574107637 7 29.151748180596236 31.820073151009176 20.513261967365867 18.51491670102872 8 26.207081401414012 34.543386918322255 19.671196167329118 19.578335512934622 9 26.229938376562206 17.088494726903797 20.285233991803178 36.39633290473082 10-11 25.344315329542894 25.905611705275287 26.97291157583907 21.777161389342744 12-13 26.27479111957977 23.32566976556498 27.412022268796292 22.987516846058952 14-15 23.09018468736415 24.77684073176613 25.99311279883822 26.139861782031502 16-17 22.68582298227702 27.906201476285688 26.41821453884615 22.989761002591145 18-19 22.403505131174647 26.62117672260863 28.597278100963308 22.37804004525341 20-21 23.23780779829086 25.772279774258383 28.48692570639317 22.502986721057592 22-23 22.83248383634758 25.752151303971736 28.511805809876485 22.9035590498042 24-25 22.863036853894425 25.856034729124666 28.626283988481838 22.654644428499076 26-27 22.596061324004854 26.013030353311965 28.679580239900034 22.711328082783147 28-29 22.286106606128268 26.42107156233876 28.649293881601682 22.643527949931283 30-31 22.52430600792323 25.959168611410632 28.541682371682047 22.974843008984095 32-33 22.45714915365386 26.048036755710847 28.619777054163553 22.87503703647174 34-35 23.016862548825177 26.142787560331215 28.23288753872804 22.60746235211557 36-37 22.918477244936753 25.917675091849823 28.428109196566208 22.735738466647216 38-39 22.626882628865758 26.177422319390164 28.481372024231792 22.714323027512286 40-41 22.795494715903764 26.1826518514419 28.350599523347302 22.671253909307033 42-43 22.687921212099223 26.207758532540947 27.960522205251802 23.143798050108025 44-45 22.727820624302826 26.177043158417113 28.062478390287566 23.03265782699249 46-47 22.919074534763265 26.49765532269363 27.728159065832507 22.855111076710596 48-49 23.043774577009003 26.40669630299424 27.6339155097214 22.915613610275347 50-51 22.62006669169778 26.64431963529473 27.788525448013452 22.94708822499404 52-53 22.927932328952657 26.742532560079464 27.355049505190003 22.974485605777875 54-55 22.668816770812334 26.354135590942683 27.623879594167843 23.353168044077137 56-57 22.753784240485604 26.614197920248095 27.55903036273327 23.072987476533026 58-59 23.142290886660334 26.417135386890305 27.41854799537477 23.022025731074592 60-61 22.906905679335175 26.38248750457092 27.929558533559888 22.78104828253401 62-63 22.71224913970891 26.509219501570634 27.877650355611173 22.900881003109287 64-65 22.50038945054744 26.64167014674389 27.642319477406424 23.215620925302243 66-67 22.65353059307899 26.579903490236184 27.379662195665006 23.38690372101982 68-69 22.474198034671108 26.51441116620393 27.710607727053127 23.300783072071827 70-71 22.71906814963654 26.369207020117198 27.53622448115456 23.375500349091702 72-73 22.682529798143193 26.350914794494663 27.697316806221313 23.269238601140838 74-75 22.490395749937907 26.867210826863303 27.333701052731175 23.308692370467615 76-77 22.668596149437537 26.49615317323231 27.338189917973242 23.49706075935691 78-79 22.69354192545207 26.510305872591466 27.455891950830168 23.340260251126296 80-81 22.757015404230906 26.650199480457363 27.29311837766195 23.29966673764978 82-83 22.772955715150847 26.59893847289352 27.4306978074108 23.197408004544833 84-85 22.676905849208946 26.54306929050086 27.291471248957492 23.4885536113327 86-87 22.574721731895103 26.53150928062024 27.49600093741467 23.397768050069985 88-89 22.728099850179575 26.48554450612748 27.153957554642528 23.632398089050422 90-91 22.75521765016578 26.43453964123909 27.243626530620475 23.566616177974655 92-93 22.838676710452326 26.42026974574786 27.32964558238653 23.411407961413282 94-95 22.681904134166846 26.278784796420616 27.364418738337797 23.674892331074737 96-97 22.58165277841621 26.186979694633216 27.519797443637195 23.711570083313376 98-99 22.615702374930045 26.29859919070564 27.134907660126473 23.95079077423784 100-101 22.79701992361673 25.793504465545553 27.46021592653015 23.949259684307563 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3255.0 1 1971.0 2 951.5 3 1216.0 4 1019.0 5 954.5 6 1732.5 7 3628.5 8 5768.5 9 6383.5 10 5144.5 11 3962.0 12 3910.0 13 4236.0 14 4565.0 15 4883.0 16 5110.5 17 5464.0 18 5698.5 19 5961.0 20 6510.0 21 7283.5 22 8480.5 23 9962.5 24 11857.0 25 14502.0 26 18213.0 27 22890.5 28 27430.0 29 32343.0 30 38295.0 31 44671.5 32 51177.5 33 57177.5 34 63831.0 35 69563.5 36 76059.5 37 83102.5 38 87497.5 39 91153.5 40 95299.0 41 101360.0 42 108340.0 43 115583.0 44 121789.0 45 126764.0 46 129885.5 47 137999.5 48 156149.0 49 156776.5 50 142497.0 51 136429.0 52 134452.0 53 134435.0 54 133153.5 55 127386.0 56 118392.0 57 108195.5 58 99093.5 59 87934.0 60 74573.5 61 61950.0 62 50924.0 63 42003.5 64 32560.5 65 24439.0 66 18869.5 67 15220.0 68 11654.0 69 8284.5 70 6393.5 71 4976.0 72 3769.5 73 3062.0 74 2514.5 75 1955.0 76 1430.5 77 934.0 78 633.0 79 439.5 80 313.0 81 234.5 82 172.5 83 124.5 84 101.5 85 72.5 86 52.5 87 45.0 88 36.0 89 27.5 90 24.0 91 21.5 92 17.5 93 13.5 94 9.5 95 9.0 96 8.5 97 4.0 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1422385249591941 2 0.081852730396164 3 0.07644584630851681 4 0.09063222535036342 5 0.10896745109312742 6 0.03070146558678879 7 0.03313724010152094 8 0.036135116427345126 9 0.03640278395643657 10-11 0.03533211384007079 12-13 0.03814262289553097 14-15 0.036657068109073446 16-17 0.035733615133707965 18-19 0.038892091976987016 20-21 0.04099328208035486 22-23 0.05100404766837492 24-25 0.06415990672321946 26-27 0.06596666254458672 28-29 0.05753513537820619 30-31 0.04731023576691298 32-33 0.03970847794071592 34-35 0.2589148008901551 36-37 0.4113113085783696 38-39 0.3904198579327822 40-41 0.36860495431182944 42-43 0.39608102617306634 44-45 0.4052352556679938 46-47 0.4042716525632646 48-49 0.4190736669220215 50-51 0.4088621506871828 52-53 0.41513895424437725 54-55 0.4076041133004531 56-57 0.38877370262886984 58-59 0.36900645560546663 60-61 0.3403794133691366 62-63 0.3936987851641525 64-65 0.4280940626524032 66-67 0.4294324002978604 68-69 0.438894447451243 70-71 0.4369672412417846 72-73 0.4279869956407666 74-75 0.4215763583190265 76-77 0.40468653723335624 78-79 0.38182773024894684 80-81 0.386391461619956 82-83 0.39693756226615895 84-85 0.39567952487942915 86-87 0.4043385694455374 88-89 0.4155270721615598 90-91 0.41951531834502237 92-93 0.42114809027248024 94-95 0.42541738736148876 96-97 0.426314073583945 98-99 0.4202380206735693 100-101 0.40867478341681884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3735978.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.74216627592187 #Duplication Level Percentage of deduplicated Percentage of total 1 72.10347525994321 35.144795801919706 2 14.765081416433674 14.393641069546682 3 5.382759381121395 7.871020583336918 4 2.533853307322162 4.940219968971655 5 1.4237128788889533 3.469742493598839 6 0.8268447549455623 2.418132271795828 7 0.5474323452710949 1.8678126878615104 8 0.3921354527258509 1.5290825151557856 9 0.28623495864139203 1.2556540753272314 >10 1.5361247890215444 13.934065055153265 >50 0.13196626139454717 4.423496553395587 >100 0.06636569672567467 5.637935220590384 >500 0.002364115019734714 0.7902509328691292 >1k 0.0013744854543269263 1.1459696914252324 >5k 1.6493825451923105E-4 0.5349716565704442 >10k+ 1.0995883634615403E-4 0.6432094224817814 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13649 0.3653394104569138 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10353 0.2771161928683734 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7610 0.20369498963858998 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6992 0.18715313634073863 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5361 0.14349656234592387 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3863 0.10339996648802535 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3802 0.10176719456056756 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.353350581828908E-5 7 0.0 0.0 0.0 0.0 5.353350581828908E-5 8 0.0 0.0 0.0 0.0 5.353350581828908E-5 9 0.0 0.0 0.0 0.0 5.353350581828908E-5 10-11 0.0 0.0 0.0 2.676675290914454E-5 5.353350581828908E-5 12-13 0.0 0.0 0.0 5.353350581828908E-5 1.4721714100029496E-4 14-15 0.0 0.0 0.0 8.030025872743362E-5 1.739838939094395E-4 16-17 0.0 0.0 0.0 1.0706701163657816E-4 2.4090077618230086E-4 18-19 0.0 0.0 0.0 1.2045038809115042E-4 2.4090077618230086E-4 20-21 0.0 0.0 0.0 1.4721714100029496E-4 2.4090077618230086E-4 22-23 0.0 0.0 0.0 2.8105090554601765E-4 2.542841526368731E-4 24-25 0.0 0.0 0.0 3.613511642734513E-4 2.676675290914454E-4 26-27 0.0 0.0 0.0 5.888685640011798E-4 2.8105090554601765E-4 28-29 0.0 0.0 0.0 0.0019138228330038347 3.078176584551622E-4 30-31 0.0 0.0 0.0 0.006357103815921828 3.2120103490973446E-4 32-33 0.0 0.0 0.0 0.015725467334122417 3.2120103490973446E-4 34-35 0.0 0.0 0.0 0.029095460412240114 3.3458441136430673E-4 36-37 0.0 0.0 0.0 0.052369152066741295 3.47967787818879E-4 38-39 0.0 0.0 0.0 0.08736668149544777 3.7473454072802355E-4 40-41 0.0 0.0 0.0 0.12295307948815545 3.7473454072802355E-4 42-43 0.0 0.0 0.0 0.16234035639396166 3.881179171825958E-4 44-45 0.0 0.0 0.0 0.21484334222524865 4.2826804654631263E-4 46-47 0.0 0.0 0.0 0.28170669099229173 4.8180155236460167E-4 48-49 0.0 0.0 0.0 0.35411075761152766 5.219516817283184E-4 50-51 0.0 0.0 0.0 0.43296561168186753 5.621018110920353E-4 52-53 0.0 0.0 0.0 0.5134933878090289 6.022519404557522E-4 54-55 0.0 0.0 0.0 0.6055442510635769 6.156353169103244E-4 56-57 0.0 0.0 0.0 0.7193163343038957 6.156353169103244E-4 58-59 0.0 0.0 0.0 0.8449327057065111 6.156353169103244E-4 60-61 0.0 2.676675290914454E-5 0.0 0.9842670379750631 6.424020698194689E-4 62-63 0.0 2.676675290914454E-5 0.0 1.124310689195707 6.691688227286135E-4 64-65 0.0 2.676675290914454E-5 0.0 1.273976988087189 6.691688227286135E-4 66-67 0.0 2.676675290914454E-5 0.0 1.4515476268864538 6.825521991831857E-4 68-69 0.0 2.676675290914454E-5 0.0 1.6533154103155854 6.95935575637758E-4 70-71 0.0 2.676675290914454E-5 0.0 1.8764965960720326 6.95935575637758E-4 72-73 0.0 2.676675290914454E-5 0.0 2.1057404513624007 7.093189520923303E-4 74-75 0.0 2.676675290914454E-5 0.0 2.342572681102512 7.494690814560471E-4 76-77 0.0 2.676675290914454E-5 0.0 2.6029462700262154 7.896192108197639E-4 78-79 0.0 2.676675290914454E-5 0.0 2.9030551036435437 8.030025872743362E-4 80-81 0.0 4.015012936371681E-5 0.0 3.2318712797559304 8.030025872743362E-4 82-83 0.0 5.353350581828908E-5 0.0 3.558626415894312 8.030025872743362E-4 84-85 0.0 5.353350581828908E-5 0.0 3.9015219040369082 8.030025872743362E-4 86-87 0.0 5.353350581828908E-5 0.0 4.266312596059184 8.163859637289085E-4 88-89 0.0 5.353350581828908E-5 0.0 4.667225021132351 8.297693401834807E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13580 0.0 25.80522 1 GTATCAA 20820 0.0 25.37274 1 TACATGG 13740 0.0 24.699347 2 ACATGGG 13870 0.0 23.34014 3 GGTATCA 15430 0.0 22.281017 1 ATCAACG 24285 0.0 21.625235 3 GTGTAGC 2065 0.0 21.585926 1 TCAACGC 24450 0.0 21.518068 4 TATCAAC 24720 0.0 21.37891 2 CAACGCA 24600 0.0 21.348326 5 GAGTACT 12035 0.0 21.194607 12-13 AACGCAG 25085 0.0 21.015404 6 AGAGTAC 18965 0.0 20.231028 10-11 CATGGGG 8370 0.0 19.763287 4 GTACTTT 12910 0.0 19.537956 14-15 ACGCAGA 27415 0.0 19.16148 7 AGTACTT 12790 0.0 19.07315 12-13 CGCAGAG 27805 0.0 18.841608 8 CCTATAC 785 0.0 18.11385 3 CATGGGA 6095 0.0 17.96377 4 >>END_MODULE