FastQCFastQC Report
Thu 26 May 2016
SRR938689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938689_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3735978
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136030.3641081398230932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120270.3219237372382814No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT102330.27390418251927606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81180.2172925001164354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199250.021.9887541
GTACATG132800.021.767161
GAGTACT136300.021.5706412-13
GTATCAA255150.020.8295441
TACATGG137000.020.7493042
GTACTTT142900.020.29221714-15
ACATGGG135650.019.9062633
AGTACTT141050.019.68341312-13
AGAGTAC214000.018.9055610-11
ATACGGT2302.0784391E-618.5700026
TCAACGC291900.017.948674
ATCAACG292600.017.8895113
CAACGCA293450.017.8700355
TATCAAC297000.017.6883962
AACGCAG297600.017.620846
ACTTTTT168000.017.44396816-17
TACCTGG28300.017.1045252
ACCGTGC6950.017.0707458
TACTTTT161250.016.9234414-15
CATGGGG79900.016.2742524
Produced by FastQC (version 0.11.5)