##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938689_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3735978 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.675449105963686 31.0 31.0 34.0 30.0 34.0 2 31.929212645256477 31.0 31.0 34.0 30.0 34.0 3 32.00207281734529 33.0 31.0 34.0 30.0 34.0 4 35.71322181233401 37.0 35.0 37.0 33.0 37.0 5 35.60086702866023 37.0 35.0 37.0 33.0 37.0 6 35.63840044025955 37.0 35.0 37.0 33.0 37.0 7 35.57306038740057 37.0 35.0 37.0 33.0 37.0 8 35.52962865413019 37.0 35.0 37.0 33.0 37.0 9 37.135473763496464 39.0 37.0 39.0 33.0 39.0 10-11 37.03310819817462 39.0 37.0 39.0 33.0 39.0 12-13 36.94184374212054 39.0 37.0 39.0 33.0 39.0 14-15 38.04480526919591 40.0 37.0 41.0 33.0 41.0 16-17 37.937073237583306 40.0 37.0 41.0 33.0 41.0 18-19 37.940162522370315 40.0 37.0 41.0 33.0 41.0 20-21 38.03953248654034 40.0 37.0 41.0 33.0 41.0 22-23 37.98381146784055 40.0 37.0 41.0 33.0 41.0 24-25 37.88759717000475 40.0 37.0 41.0 32.0 41.0 26-27 37.68564830949219 40.0 37.0 41.0 32.0 41.0 28-29 37.49107596458009 39.5 36.5 41.0 32.0 41.0 30-31 37.233096527870345 39.0 36.0 41.0 31.0 41.0 32-33 36.973256132664595 39.0 36.0 40.5 31.0 41.0 34-35 36.92707331253021 39.0 36.0 41.0 30.0 41.0 36-37 36.83109737262907 39.0 35.0 41.0 30.0 41.0 38-39 36.70687314004526 39.0 35.0 41.0 30.0 41.0 40-41 36.51853048920523 39.0 35.0 40.5 30.0 41.0 42-43 36.30248304460037 39.0 35.0 40.0 30.0 41.0 44-45 36.01712965119174 38.5 35.0 40.0 29.5 41.0 46-47 35.699822375827694 38.0 35.0 40.0 28.0 41.0 48-49 35.391564939622235 38.0 34.5 40.0 27.0 41.0 50-51 35.11242959674816 38.0 34.0 40.0 26.5 41.0 52-53 34.85712348948522 38.0 34.0 40.0 26.0 41.0 54-55 34.57240660946076 38.0 33.5 40.0 25.5 41.0 56-57 34.248096616200634 37.5 33.0 40.0 24.5 41.0 58-59 33.88729256435664 37.0 33.0 40.0 23.5 41.0 60-61 33.46738243640621 36.5 32.0 39.0 23.0 41.0 62-63 33.03526747213179 36.0 32.0 39.0 22.0 40.0 64-65 32.62529128918854 35.5 31.0 39.0 21.5 40.0 66-67 32.170528306108864 35.0 31.0 38.0 20.0 40.0 68-69 31.663808378957263 35.0 30.5 38.0 18.5 40.0 70-71 31.121910380628577 34.5 30.0 37.0 17.5 39.0 72-73 30.685993600604714 34.0 29.5 36.5 14.0 39.0 74-75 30.20875122926313 34.0 29.0 36.0 10.0 39.0 76-77 28.319327362206096 31.5 26.5 34.5 7.5 36.5 78-79 29.406662057431816 33.5 29.0 35.0 7.0 37.0 80-81 29.455875007829274 34.0 29.0 35.0 7.0 37.0 82-83 29.083879776593974 34.0 29.0 35.0 2.0 36.5 84-85 28.617504037764675 33.5 28.5 35.0 2.0 36.0 86-87 27.966626007968998 33.0 26.5 35.0 2.0 35.5 88-89 27.3381437203324 33.0 26.0 35.0 2.0 35.0 90-91 26.78605053348815 32.0 25.0 34.5 2.0 35.0 92-93 26.35364180410056 32.0 25.0 34.0 2.0 35.0 94-95 25.773603859551635 32.0 23.5 34.0 2.0 35.0 96-97 24.983538714628406 31.5 19.0 34.0 2.0 35.0 98-99 23.916366611366556 31.0 4.5 34.0 2.0 35.0 100-101 20.487190101226506 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09309178009640107 1101 2 0.16277045247584354 1101 3 0.17264435436417358 1101 4 0.21007556119359805 1101 5 0.08244779903839827 1101 6 0.22933734553107143 1101 7 0.2385392518420275 1101 8 0.20351152491272728 1101 9 0.2773337267671465 1101 10-11 0.28279865515692393 1101 12-13 0.30966438003101615 1101 14-15 0.4476602205097393 1101 16-17 0.3172581528104885 1101 18-19 0.3515066545883414 1101 20-21 0.3576005257956112 1101 22-23 0.3299703890815877 1101 24-25 0.3941646826018186 1101 26-27 0.40174456258119307 1101 28-29 0.39899437907701696 1101 30-31 0.29460831288853484 1101 32-33 0.32060579237127484 1101 34-35 0.3731237592972221 1101 36-37 0.37524390657626583 1101 38-39 0.4412599004853206 1101 40-41 0.4354102978471346 1101 42-43 0.381885621966255 1101 44-45 0.45935804535161395 1101 46-47 0.47784282196785455 1101 48-49 0.4474316074270135 1101 50-51 0.46937948518531414 1101 52-53 0.49688917232405316 1101 54-55 0.4102370146554932 1101 56-57 0.34166435106003945 1101 58-59 0.5456632680374014 1101 60-61 0.5698090126199489 1101 62-63 0.4044471665133358 1101 64-65 0.5363052120728824 1101 66-67 0.39197852243501785 1101 68-69 0.46422059307367647 1101 70-71 0.3721491641361716 1101 72-73 0.4534038375765199 1101 74-75 0.5348279725456244 1101 76-77 0.536011184339305 1101 78-79 0.6287521451006661 1101 80-81 0.6129784141813026 1101 82-83 0.6415245444990951 1101 84-85 0.7711675507465721 1101 86-87 0.8087378283628439 1101 88-89 0.8108447304615076 1101 90-91 0.8988246196331815 1101 92-93 1.0156068793787192 1101 94-95 1.0803264250514069 1101 96-97 1.14359653166958 1101 98-99 1.4277379185178383 1101 100-101 1.8100734324074956 1102 1 0.15584166226161855 1102 2 0.13229433645842548 1102 3 0.22483163646242588 1102 4 0.16963498147425327 1102 5 0.11776463272421012 1102 6 0.11063522273234838 1102 7 0.21095175811078093 1102 8 0.17095162165168176 1102 9 0.1993301436373045 1102 10-11 0.25187388725804283 1102 12-13 0.20667195201539812 1102 14-15 0.41298474801640594 1102 16-17 0.4125535850805804 1102 18-19 0.2802164130015754 1102 20-21 0.25671748258785243 1102 22-23 0.34322906748266035 1102 24-25 0.14292293616269092 1102 26-27 0.2440638315412187 1102 28-29 0.3037240482943204 1102 30-31 0.27207031902586465 1102 32-33 0.4285102408310806 1102 34-35 0.35414471979984796 1102 36-37 0.3958336083123868 1102 38-39 0.32367120558672724 1102 40-41 0.389089917939593 1102 42-43 0.4037079423279053 1102 44-45 0.2366665124041134 1102 46-47 0.17866308830687672 1102 48-49 0.33497677852920305 1102 50-51 0.29126475438901167 1102 52-53 0.35879874664647815 1102 54-55 0.3014523605473727 1102 56-57 0.12210825515555968 1102 58-59 0.12144647743751591 1102 60-61 0.19428351843813374 1102 62-63 0.09238534290582123 1102 64-65 0.18239057723767615 1102 66-67 0.14572644899924825 1102 68-69 0.22546516648927195 1102 70-71 0.17930746674911902 1102 72-73 0.2842340995328634 1102 74-75 0.3134642388306723 1102 76-77 0.25829891075042255 1102 78-79 0.19891827659965244 1102 80-81 0.2898870499263744 1102 82-83 0.4218930449170557 1102 84-85 0.6367565599594442 1102 86-87 0.6647895621645823 1102 88-89 0.7683743366571996 1102 90-91 0.8452081732709296 1102 92-93 0.9707602445059464 1102 94-95 0.9941206415364263 1102 96-97 1.0941171345338887 1102 98-99 1.3501181004206124 1102 100-101 1.6752613138988401 1103 1 0.1273142690793172 1103 2 0.12673046437983615 1103 3 0.06981108760813015 1103 4 0.07603576355064234 1103 5 0.10349245431770271 1103 6 0.13688167022669973 1103 7 0.2017163202115526 1103 8 0.2652737818057176 1103 9 0.29214280571535767 1103 10-11 0.031740496133501495 1103 12-13 0.12321430155379431 1103 14-15 0.21709516826451392 1103 16-17 0.15699814511921062 1103 18-19 0.17282407357841123 1103 20-21 0.2512615548624595 1103 22-23 0.16446669156979254 1103 24-25 0.19837509014288912 1103 26-27 0.2620004523687456 1103 28-29 0.21853547799110373 1103 30-31 0.28684948469221894 1103 32-33 0.30981803603685165 1103 34-35 0.14977590899873405 1103 36-37 -0.056924601836378486 1103 38-39 0.1734946678650786 1103 40-41 0.1455480417262578 1103 42-43 0.3081041997533944 1103 44-45 0.30732029934581817 1103 46-47 0.35299268860331523 1103 48-49 0.4638250295093016 1103 50-51 0.45313347457386044 1103 52-53 0.45482041741964707 1103 54-55 0.40576871662166525 1103 56-57 0.3520945338312984 1103 58-59 0.1344599014343828 1103 60-61 0.3962323612965406 1103 62-63 0.5207381896051544 1103 64-65 0.5345053224164431 1103 66-67 0.40758497112641834 1103 68-69 0.4460612472676928 1103 70-71 0.435426748638875 1103 72-73 0.4087063633008903 1103 74-75 0.500996447364848 1103 76-77 0.5958867911374739 1103 78-79 0.4504972029727945 1103 80-81 0.6227251468482073 1103 82-83 0.5990903933690497 1103 84-85 0.39132625394283593 1103 86-87 0.538465313893628 1103 88-89 0.6793173959114931 1103 90-91 0.8619242741220958 1103 92-93 0.8387686857676186 1103 94-95 0.822848603146138 1103 96-97 1.042514830157188 1103 98-99 1.435677683881309 1103 100-101 1.7735109852781967 1104 1 -0.07665260800109408 1104 2 -0.012652442782911777 1104 3 -0.05792951463149265 1104 4 -0.052810087950248885 1104 5 -0.006461714342663072 1104 6 0.06277075628123896 1104 7 -0.11390750465434962 1104 8 -0.08140233624580873 1104 9 -0.04803649831977452 1104 10-11 0.05613400912692157 1104 12-13 -0.007101641422643468 1104 14-15 0.018154057567890902 1104 16-17 0.09611344441071168 1104 18-19 0.08402228452659699 1104 20-21 0.008135026462483097 1104 22-23 0.0049161441220704205 1104 24-25 -0.014718374346045948 1104 26-27 0.035957445517063036 1104 28-29 0.042131876994353945 1104 30-31 -0.12791983627099768 1104 32-33 -0.06349617370002392 1104 34-35 -0.025027289974950406 1104 36-37 -0.16489156879555367 1104 38-39 0.09498215320342496 1104 40-41 0.10143402846599514 1104 42-43 0.10520196940777993 1104 44-45 0.021290286668538272 1104 46-47 -0.08351342882124158 1104 48-49 -0.03483676039212469 1104 50-51 -0.09986876979994008 1104 52-53 -0.1354077189223375 1104 54-55 -0.10816966288062702 1104 56-57 -0.09895565444285381 1104 58-59 -0.09035857556314397 1104 60-61 0.023436832614763148 1104 62-63 -0.18392576011846273 1104 64-65 0.06723963245965336 1104 66-67 -0.1597024645737264 1104 68-69 -0.12581758632450502 1104 70-71 -0.12492834350529947 1104 72-73 -0.21606561031518723 1104 74-75 -0.2765487300316529 1104 76-77 -0.1795630705134279 1104 78-79 -0.1298164042485439 1104 80-81 -0.08880199578706538 1104 82-83 -0.08768553108798827 1104 84-85 -0.051524921713731686 1104 86-87 0.24300489490287447 1104 88-89 0.3945824638198161 1104 90-91 0.3985948778792725 1104 92-93 0.3879112314374389 1104 94-95 0.5053719166455863 1104 96-97 0.6781614853217377 1104 98-99 0.7168938214083838 1104 100-101 1.3758227954706364 1105 1 -0.06518150321588223 1105 2 -0.040917980775770246 1105 3 -0.05314083577359341 1105 4 -0.09163266586563878 1105 5 -0.029148252105237304 1105 6 -0.09575200724938782 1105 7 -0.045284386817805 1105 8 -0.039045161481709556 1105 9 0.005082614500437899 1105 10-11 -3.6876677805253166E-4 1105 12-13 -0.008536760713028002 1105 14-15 0.10643990503044876 1105 16-17 -0.057712338072640534 1105 18-19 -0.07991890402130508 1105 20-21 -0.0013764364574768706 1105 22-23 -0.02714729245106895 1105 24-25 -0.04933464467286086 1105 26-27 0.030428455968667834 1105 28-29 -0.007056021669164636 1105 30-31 -0.10075772397517113 1105 32-33 -0.09469416883352011 1105 34-35 0.0012969179696042943 1105 36-37 -0.08328835534913281 1105 38-39 0.07919459247617766 1105 40-41 0.047010476139980995 1105 42-43 0.11662507503644548 1105 44-45 0.13577861011071235 1105 46-47 0.3250167565442865 1105 48-49 0.14001161221450076 1105 50-51 0.2804786275637099 1105 52-53 0.1451836279117984 1105 54-55 0.19371582999858106 1105 56-57 0.24883506646008158 1105 58-59 0.25115299556300386 1105 60-61 0.14039440746431353 1105 62-63 0.05384707901777119 1105 64-65 0.08876004042473795 1105 66-67 0.005707484291910703 1105 68-69 0.09620826940611948 1105 70-71 0.03161230456655062 1105 72-73 -0.10663689738621329 1105 74-75 0.0322569649434179 1105 76-77 0.012009748091376338 1105 78-79 -0.12410755658898509 1105 80-81 -0.029498205518411424 1105 82-83 0.13796405287308033 1105 84-85 0.22521483962225375 1105 86-87 0.20758408380036641 1105 88-89 0.49339927073835455 1105 90-91 0.6119543194243455 1105 92-93 0.6118200136183738 1105 94-95 0.6233088268871647 1105 96-97 0.7411976261895532 1105 98-99 0.97135961576668 1105 100-101 1.4378900053878745 1106 1 -0.04601903898470283 1106 2 -0.1753080181164961 1106 3 -0.10448091011285854 1106 4 -0.0556647369712735 1106 5 -0.029008170922310228 1106 6 -0.11183140226977173 1106 7 -0.06765610025662028 1106 8 -0.08685240977400355 1106 9 -0.10827556987634068 1106 10-11 -0.13698256616468996 1106 12-13 -0.017165119988113986 1106 14-15 0.01214815976862127 1106 16-17 -0.13074018749500027 1106 18-19 -0.21308617164693544 1106 20-21 -0.11981283104585572 1106 22-23 -0.13497066611923003 1106 24-25 -0.2100002762358315 1106 26-27 -9.571073287801823E-4 1106 28-29 -0.04888383516229311 1106 30-31 -0.10161253998762021 1106 32-33 -0.12853831728963172 1106 34-35 -0.0940346360119122 1106 36-37 -0.14103249225657066 1106 38-39 -0.13156791966063253 1106 40-41 0.04712596291674487 1106 42-43 0.0010360710885066737 1106 44-45 0.005751299626659545 1106 46-47 -0.04367445747837451 1106 48-49 -0.015395628378669812 1106 50-51 0.09796482261659634 1106 52-53 0.06214831462184378 1106 54-55 0.0028088096857388223 1106 56-57 -0.033182680007946885 1106 58-59 -0.11150702847535143 1106 60-61 -0.06810926886474533 1106 62-63 -0.06989293886128678 1106 64-65 -0.039812885197498815 1106 66-67 -0.1184046576456197 1106 68-69 -0.18623378403431445 1106 70-71 -0.05037624941068586 1106 72-73 0.09392674224371689 1106 74-75 0.262598869961959 1106 76-77 0.1129612308132728 1106 78-79 0.015159444561604118 1106 80-81 0.039967304569575646 1106 82-83 0.26703421322860876 1106 84-85 -0.009944574902561953 1106 86-87 0.19909142148797443 1106 88-89 0.4233668061222211 1106 90-91 0.4796208021676307 1106 92-93 0.681598086529025 1106 94-95 0.7013279506419394 1106 96-97 0.7351541390021694 1106 98-99 0.8814817109534481 1106 100-101 1.396898829433077 1107 1 -0.1086337767439538 1107 2 -0.06490926651584061 1107 3 -0.12958669535426637 1107 4 -0.027760893249961782 1107 5 -0.07943070642126315 1107 6 -0.09750445580746714 1107 7 -0.06129877308124776 1107 8 -0.023110555671628674 1107 9 -0.06729802276920793 1107 10-11 -0.038125833149081245 1107 12-13 -0.1278536566947892 1107 14-15 -0.15372673155740557 1107 16-17 -0.10213630028220422 1107 18-19 -0.07249323448139222 1107 20-21 -0.04615332332965494 1107 22-23 -0.13124245033615267 1107 24-25 -0.09866961658498496 1107 26-27 -0.16448643533225749 1107 28-29 -0.10912000072286077 1107 30-31 -0.18626776027026182 1107 32-33 -0.018378579131358208 1107 34-35 -0.1496438325109466 1107 36-37 -0.19703257950212816 1107 38-39 -0.23719592210058948 1107 40-41 -0.20768826312832545 1107 42-43 -0.26518784624588676 1107 44-45 -0.342145622808097 1107 46-47 -0.44052088063655503 1107 48-49 -0.6430231531564843 1107 50-51 -0.5922842970129665 1107 52-53 -0.5432452700866648 1107 54-55 -0.5030925937860218 1107 56-57 -0.47770281994515784 1107 58-59 -0.339891612739315 1107 60-61 -0.36629726758144443 1107 62-63 -0.3784800466228333 1107 64-65 -0.2809190903263996 1107 66-67 -0.26977800196780777 1107 68-69 -0.13917049438357765 1107 70-71 -0.28464013396526155 1107 72-73 -0.20342297691457745 1107 74-75 -0.08490836536484636 1107 76-77 -0.07774111345158019 1107 78-79 0.015295966355413526 1107 80-81 -0.07000203985007047 1107 82-83 0.20206614320689553 1107 84-85 0.1056469288388584 1107 86-87 0.1336631967906854 1107 88-89 0.23996212255296356 1107 90-91 0.5383990331457262 1107 92-93 0.5695732556840163 1107 94-95 0.6752673152695827 1107 96-97 0.8065827208518535 1107 98-99 0.5504688104281215 1107 100-101 1.2255846224273732 1108 1 -0.12548562664350627 1108 2 -0.081053763016115 1108 3 -0.17119439785222923 1108 4 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-1.1876788911662857 2316 66-67 -1.2496692152749809 2316 68-69 -1.3740562156089808 2316 70-71 -1.4262002294175389 2316 72-73 -1.507816063672255 2316 74-75 -1.6820497379149408 2316 76-77 -1.583203334858073 2316 78-79 -1.5452557003871732 2316 80-81 -1.5654936438569536 2316 82-83 -1.668177805228222 2316 84-85 -1.8573614188583605 2316 86-87 -2.183412978524462 2316 88-89 -2.3665967272954624 2316 90-91 -2.7780185078052604 2316 92-93 -2.947346555174118 2316 94-95 -2.7642728617975685 2316 96-97 -2.8207331267455835 2316 98-99 -3.2201613271313505 2316 100-101 -3.4650335324082313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 25.0 4 69.0 5 146.0 6 389.0 7 942.0 8 2086.0 9 3730.0 10 5745.0 11 8377.0 12 11236.0 13 15014.0 14 18700.0 15 23621.0 16 28759.0 17 36165.0 18 34782.0 19 28731.0 20 28230.0 21 29810.0 22 32539.0 23 36113.0 24 42184.0 25 50522.0 26 60938.0 27 72961.0 28 87772.0 29 105316.0 30 125895.0 31 151154.0 32 184007.0 33 229204.0 34 295492.0 35 401848.0 36 553074.0 37 662572.0 38 350733.0 39 17090.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.509183126734804 20.51074218356585 15.533362607613194 22.446712082086155 2 17.05018070234889 21.64126769483118 37.91655090046034 23.392000702359596 3 20.67405107845924 24.486787663096518 29.65686628775651 25.182294970687728 4 14.676960089165409 17.85147021743704 35.451868292586305 32.01970140081124 5 14.328242832265072 37.16914285897829 33.733817490359954 14.768796818396682 6 32.51991847917734 36.211348139630374 16.6430583906008 14.625674990591486 7 28.910127415097197 32.33961763158134 20.3042416202665 18.44601333305496 8 26.247263768683865 35.404089638643484 19.133490614773425 19.21515597789923 9 26.102321801680844 17.07515408281312 19.976482730894027 36.84604138461201 10-11 25.566250657792956 26.45683406058601 26.65385074537377 21.32306453624727 12-13 26.423134718673396 23.687010469547733 27.27313972405619 22.61671508772268 14-15 23.00201513903817 25.275775062999024 25.831360285098608 25.890849512864193 16-17 22.575258893162136 28.348921706767626 26.38965026938594 22.6861691306843 18-19 22.373253868069675 26.933526268398154 28.69341633181405 21.99980353171812 20-21 23.214767754891458 25.94156415556571 28.698968342287927 22.1446997472549 22-23 22.866328441976908 25.959588091792828 28.68647781116484 22.48760565506542 24-25 22.954511509436085 26.144332220371748 28.75939044608935 22.14176582410282 26-27 22.73833349438018 26.166530111432817 28.82358892850671 22.271547465680293 28-29 22.349395526419052 26.61241581187041 28.81530619291655 22.22288246879398 30-31 22.66179029962168 26.121647397281244 28.679411388396826 22.537150914700245 32-33 22.583684914632794 26.220724533174444 28.749299380242604 22.446291171950154 34-35 23.08277719079916 26.287180752859634 28.481921608864518 22.148120447476686 36-37 23.06102518193617 26.035772570150904 28.606959513580428 22.2962427343325 38-39 22.726201338555708 26.36261145651956 28.67469386056058 22.236493344364156 40-41 22.869644917843303 26.285298684834302 28.499553099035342 22.345503298287053 42-43 22.801706537126094 26.391821234655012 28.07722539558729 22.7292468326316 44-45 22.971378876161967 26.26265019090385 28.075179501016084 22.690791431918097 46-47 23.115201131715125 26.671511520916187 27.766809937839987 22.446477409528704 48-49 23.228361210029185 26.644777696644734 27.639511067522914 22.487350025803163 50-51 22.84545378016639 26.776993316187394 27.724579436137876 22.652973467508343 52-53 23.172647162269158 26.937123291411247 27.249036263061505 22.641193283258094 54-55 22.927236188221663 26.5760933281727 27.425710215638315 23.070960267967315 56-57 23.007536446949096 26.799582331587608 27.43141153400796 22.76146968745533 58-59 23.387289970484183 26.55688774219659 27.283848903214512 22.77197338410471 60-61 23.09568462472318 26.627940295327395 27.79069504441367 22.485680035535754 62-63 22.879698435055023 26.688500307014657 27.740340012708852 22.691461245221465 64-65 22.712740813784237 26.829373727575483 27.497779697846187 22.960105760794093 66-67 22.869661799839587 26.677995368499772 27.302724603202115 23.149618228458525 68-69 22.64503256731748 26.652616707807432 27.553256812048545 23.149093912826547 70-71 22.91328786296683 26.52627349726827 27.41732743776421 23.14311120200069 72-73 22.86885790012682 26.51370002714149 27.525135319319332 23.09230675341236 74-75 22.632949123362074 27.003197020967466 27.10711893913722 23.256734916533233 76-77 22.793269659649486 26.60330998500624 27.288436537189583 23.314983818154687 78-79 22.742954820940916 26.669075595286067 27.332633235664172 23.25533634810884 80-81 22.77922823915357 26.72530551588126 27.268724424353902 23.22674182061127 82-83 22.791515367595846 26.705109077194777 27.305420428064618 23.197955127144752 84-85 22.609755731966303 26.63025585268436 27.284716344689397 23.475272070659944 86-87 22.60627430599715 26.51659572175586 27.360644276191042 23.51648569605595 88-89 22.598681362346134 26.57383088871453 27.11913984499329 23.70834790394605 90-91 22.619266946942478 26.45624824469793 27.214458227735662 23.710026580623932 92-93 22.635398281253263 26.50393016232965 27.24180923977604 23.61886231664105 94-95 22.444875537928162 26.326684247964554 27.310616833211654 23.917823380895626 96-97 22.234200348958296 26.26819253279564 27.495127445260962 24.002479672985093 98-99 22.26343566371366 26.367174691666506 27.08026909202454 24.289120552595296 100-101 22.39251339818424 25.675530266164948 27.597114418432344 24.334841917218466 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2173.0 1 1437.5 2 876.0 3 1102.5 4 954.0 5 865.0 6 1571.5 7 3593.0 8 5526.5 9 5986.5 10 4883.5 11 3499.0 12 3213.0 13 3411.0 14 3774.5 15 4072.5 16 4356.0 17 4776.0 18 5125.0 19 5420.0 20 6050.0 21 7154.0 22 8591.0 23 10260.5 24 12281.0 25 14965.5 26 18864.5 27 23859.0 28 28750.5 29 33584.5 30 39156.5 31 45735.5 32 52611.5 33 58522.0 34 64764.0 35 70747.0 36 77580.5 37 84677.0 38 89240.5 39 92486.0 40 96535.0 41 103043.0 42 109977.0 43 116375.0 44 122232.5 45 127581.5 46 131024.0 47 139801.5 48 156941.5 49 156914.0 50 143184.5 51 136253.0 52 133691.0 53 133801.5 54 132112.5 55 125362.5 56 116355.0 57 106621.0 58 97625.0 59 86751.0 60 73502.0 61 60825.5 62 50333.0 63 41343.0 64 32177.0 65 24419.5 66 18446.0 67 14706.5 68 10997.0 69 7809.5 70 5905.0 71 4453.0 72 3408.0 73 2791.5 74 2277.0 75 1738.5 76 1248.5 77 763.5 78 455.0 79 303.0 80 200.0 81 113.0 82 71.0 83 41.0 84 22.0 85 16.5 86 8.5 87 4.0 88 2.0 89 2.5 90 2.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33653838432667427 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.0075064681858402E-4 16-17 0.001980739715276696 18-19 1.6060051745486726E-4 20-21 1.338337645457227E-5 22-23 0.0 24-25 0.0 26-27 1.338337645457227E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007360857050014748 36-37 0.016113585251305015 38-39 0.010291816493566076 40-41 0.0067184549801952795 42-43 0.0029041926906421827 44-45 0.0017666056920035396 46-47 0.001485554786457522 48-49 5.353350581828908E-5 50-51 3.078176584551622E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.0150129363716804E-4 60-61 6.022519404557522E-4 62-63 0.0 64-65 0.0 66-67 0.0010572867399112093 68-69 6.42402069819469E-4 70-71 0.001739838939094395 72-73 0.0 74-75 0.0 76-77 0.08273603324216577 78-79 0.033752875418431263 80-81 5.353350581828908E-5 82-83 0.0 84-85 0.0 86-87 0.13467691726236075 88-89 0.30247769124978785 90-91 0.2614442590400693 92-93 0.0 94-95 1.338337645457227E-5 96-97 4.0150129363716815E-5 98-99 1.8736727036401177E-4 100-101 6.42402069819469E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3735978.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.607071266449196 #Duplication Level Percentage of deduplicated Percentage of total 1 71.7718588848714 34.8861985974248 2 15.05971769329869 14.640175423415508 3 5.435299090223148 7.92581910698829 4 2.5313352194534247 4.921631656449818 5 1.400312569060753 3.4032546419820293 6 0.8570168623940235 2.499424782416099 7 0.5307052389109289 1.8057219158455033 8 0.4027362365530183 1.5660663161371282 9 0.28957956302493404 1.2668053011534166 >10 1.5155080177393074 13.733865544719375 >50 0.1348675538810648 4.565115576726046 >100 0.06709393637425726 5.590765688545848 >500 0.002425582031740125 0.7915406703771588 >1k 0.0013230447285705602 1.2251195208206909 >5k 5.512686369043998E-5 0.21752630673043827 >10k+ 1.6538059107131995E-4 0.9609689502677639 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13603 0.3641081398230932 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12027 0.3219237372382814 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10233 0.27390418251927606 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8118 0.2172925001164354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.030025872743362E-5 7 0.0 0.0 0.0 0.0 8.030025872743362E-5 8 0.0 0.0 0.0 0.0 8.030025872743362E-5 9 0.0 0.0 0.0 0.0 8.030025872743362E-5 10-11 0.0 0.0 0.0 1.338337645457227E-5 8.030025872743362E-5 12-13 0.0 0.0 0.0 2.676675290914454E-5 8.030025872743362E-5 14-15 0.0 0.0 0.0 5.353350581828908E-5 9.368363518200589E-5 16-17 0.0 0.0 0.0 8.030025872743362E-5 1.0706701163657816E-4 18-19 0.0 0.0 0.0 9.368363518200589E-5 1.0706701163657816E-4 20-21 0.0 0.0 0.0 1.0706701163657816E-4 1.0706701163657816E-4 22-23 0.0 0.0 0.0 2.0075064681858405E-4 2.1413402327315632E-4 24-25 0.0 0.0 0.0 2.8105090554601765E-4 2.542841526368731E-4 26-27 0.0 0.0 0.0 5.085683052737462E-4 2.676675290914454E-4 28-29 0.0 0.0 0.0 0.0018201391978218287 2.676675290914454E-4 30-31 0.0 0.0 0.0 0.006316953686558111 2.676675290914454E-4 32-33 0.0 0.0 0.0 0.0156853172047587 3.47967787818879E-4 34-35 0.0 0.0 0.0 0.02894824327123982 3.7473454072802355E-4 36-37 0.0 0.0 0.0 0.052221934925740995 3.7473454072802355E-4 38-39 0.0 0.0 0.0 0.08708563058990176 3.7473454072802355E-4 40-41 0.0 0.0 0.0 0.12269879533551857 3.7473454072802355E-4 42-43 0.0 0.0 0.0 0.16200577198259733 4.015012936371681E-4 44-45 0.0 0.0 0.0 0.21401357288506517 4.2826804654631263E-4 46-47 0.0 0.0 0.0 0.28026128633519787 4.2826804654631263E-4 48-49 0.0 0.0 0.0 0.35221031815497844 4.2826804654631263E-4 50-51 0.0 0.0 0.0 0.43055660392004447 4.2826804654631263E-4 52-53 0.0 0.0 0.0 0.5105088948596592 4.6841817591002945E-4 54-55 0.0 0.0 0.0 0.60122142046875 5.085683052737462E-4 56-57 0.0 0.0 0.0 0.7137488496987937 5.353350581828908E-4 58-59 0.0 0.0 0.0 0.837866282938497 5.487184346374631E-4 60-61 0.0 0.0 0.0 0.9753537092563178 5.621018110920353E-4 62-63 0.0 0.0 0.0 1.1136709049143223 6.156353169103244E-4 64-65 0.0 0.0 0.0 1.2610620298085267 6.156353169103244E-4 66-67 0.0 0.0 0.0 1.4362638109753323 6.290186933648966E-4 68-69 0.0 0.0 0.0 1.6347392837966392 6.424020698194689E-4 70-71 0.0 0.0 0.0 1.8544809418042612 6.691688227286135E-4 72-73 0.0 0.0 0.0 2.0816771404970797 6.825521991831857E-4 74-75 0.0 0.0 0.0 2.3156720944288214 6.95935575637758E-4 76-77 0.0 0.0 0.0 2.5728069062505186 6.95935575637758E-4 78-79 0.0 0.0 0.0 2.8671207378630177 6.95935575637758E-4 80-81 0.0 0.0 0.0 3.1898608610650276 7.360857050014748E-4 82-83 0.0 0.0 0.0 3.510633627928216 8.030025872743362E-4 84-85 0.0 0.0 0.0 3.8448165379988852 8.030025872743362E-4 86-87 0.0 0.0 0.0 4.199556314303778 8.297693401834807E-4 88-89 0.0 0.0 0.0 4.589962788860106 8.565360930926253E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19925 0.0 21.988754 1 GTACATG 13280 0.0 21.76716 1 GAGTACT 13630 0.0 21.57064 12-13 GTATCAA 25515 0.0 20.829544 1 TACATGG 13700 0.0 20.749304 2 GTACTTT 14290 0.0 20.292217 14-15 ACATGGG 13565 0.0 19.906263 3 AGTACTT 14105 0.0 19.683413 12-13 AGAGTAC 21400 0.0 18.90556 10-11 ATACGGT 230 2.0784391E-6 18.570002 6 TCAACGC 29190 0.0 17.94867 4 ATCAACG 29260 0.0 17.889511 3 CAACGCA 29345 0.0 17.870035 5 TATCAAC 29700 0.0 17.688396 2 AACGCAG 29760 0.0 17.62084 6 ACTTTTT 16800 0.0 17.443968 16-17 TACCTGG 2830 0.0 17.104525 2 ACCGTGC 695 0.0 17.070745 8 TACTTTT 16125 0.0 16.92344 14-15 CATGGGG 7990 0.0 16.274252 4 >>END_MODULE