FastQCFastQC Report
Thu 26 May 2016
SRR938688_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938688_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3676918
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135770.36924946381725127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103830.2823832350898225No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT74220.20185383519567202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69970.19029524183025023No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54040.14697091422762215No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38050.1034834064833646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA210050.024.6180021
GTACATG134050.024.6059021
TACATGG137250.023.368372
GGTATCA155900.022.9864481
ACATGGG136400.022.4009063
GAGTACT115300.021.75923712-13
ATCAACG242100.021.1652343
TCAACGC243500.021.0630284
TATCAAC246700.020.8667432
CAACGCA246100.020.8405025
AACGCAG249850.020.5846756
GTACTTT122300.020.20357514-15
AGAGTAC185250.019.7428510-11
AGTACTT121300.019.41104512-13
ACGCAGA274000.018.7543327
CATGGGG78400.018.518234
CGCAGAG275450.018.5005748
ATATGCG1300.00761655618.248235
GCAGAGT287150.017.4313819
GTGTAGC23350.016.6906641
Produced by FastQC (version 0.11.5)