##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938688_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3676918 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.042737966960374 33.0 31.0 34.0 31.0 34.0 2 32.16451060371757 34.0 31.0 34.0 31.0 34.0 3 32.194676628633005 34.0 31.0 34.0 30.0 34.0 4 35.72099241810669 37.0 35.0 37.0 35.0 37.0 5 35.733379966591585 37.0 35.0 37.0 35.0 37.0 6 35.79443300068155 37.0 36.0 37.0 35.0 37.0 7 35.72575347070563 37.0 35.0 37.0 35.0 37.0 8 35.701841868651954 37.0 35.0 37.0 35.0 37.0 9 37.37932801329809 39.0 37.0 39.0 35.0 39.0 10-11 37.273988024753336 39.0 37.0 39.0 35.0 39.0 12-13 37.1807568186182 39.0 37.0 39.0 34.0 39.0 14-15 38.4598172980741 40.0 38.0 41.0 34.0 41.0 16-17 38.34442826845744 40.0 38.0 41.0 34.0 41.0 18-19 38.27415569234887 40.0 38.0 41.0 34.0 41.0 20-21 38.162612954653866 40.0 38.0 41.0 34.0 41.0 22-23 38.19466357422167 40.0 38.0 41.0 34.0 41.0 24-25 38.14304534395382 40.0 38.0 41.0 34.0 41.0 26-27 38.01994550871137 40.0 38.0 41.0 33.5 41.0 28-29 37.858628612332396 40.0 38.0 41.0 33.0 41.0 30-31 37.65243717700531 40.0 37.5 41.0 33.0 41.0 32-33 37.48725427110422 40.0 37.0 41.0 32.5 41.0 34-35 37.219101704199005 40.0 37.0 41.0 31.5 41.0 36-37 37.026802746212994 40.0 36.5 41.0 31.0 41.0 38-39 36.86968229914292 39.5 36.0 41.0 31.0 41.0 40-41 36.78123471885965 39.5 36.0 41.0 31.0 41.0 42-43 36.79968617739095 40.0 36.5 41.0 31.0 41.0 44-45 36.64651863326841 40.0 36.0 41.0 30.5 41.0 46-47 36.37479106142699 40.0 36.0 41.0 30.0 41.0 48-49 36.10985382322913 39.0 36.0 41.0 29.0 41.0 50-51 34.99903288569394 38.0 34.0 40.0 27.5 40.5 52-53 35.085853016031365 38.5 34.5 39.5 27.5 40.5 54-55 35.5706157983398 39.0 35.0 40.0 28.0 41.0 56-57 35.44703539758025 39.0 35.0 41.0 28.0 41.0 58-59 35.22483680626003 38.5 35.0 40.0 27.0 41.0 60-61 34.91077391445771 38.0 34.5 40.0 26.5 41.0 62-63 34.52156140006386 37.5 34.0 40.0 26.0 41.0 64-65 34.130156832434125 37.0 34.0 40.0 26.0 41.0 66-67 33.73717648857004 36.5 34.0 39.0 24.5 41.0 68-69 33.31240742926549 36.0 33.0 39.0 23.5 41.0 70-71 32.889169135672866 35.5 33.0 39.0 22.5 40.0 72-73 32.468729245525736 35.0 32.5 38.0 21.5 40.0 74-75 32.00774031403475 35.0 32.0 37.0 20.0 39.0 76-77 31.560035469923452 35.0 31.5 36.5 18.5 39.0 78-79 31.132188153230505 35.0 31.0 36.0 17.5 38.5 80-81 30.67415319025336 34.5 31.0 36.0 13.5 37.5 82-83 30.278122465608426 34.0 31.0 35.0 7.5 37.0 84-85 29.948300995562043 34.0 30.0 35.0 4.5 36.5 86-87 29.552410877805816 34.0 30.0 35.0 2.0 36.0 88-89 29.12897975967917 34.0 29.5 35.0 2.0 36.0 90-91 28.704349947428796 34.0 29.0 35.0 2.0 35.5 92-93 28.264822604148364 34.0 29.0 35.0 2.0 35.0 94-95 27.778325488901302 34.0 27.5 35.0 2.0 35.0 96-97 27.20397463310305 33.5 26.5 35.0 2.0 35.0 98-99 26.46846190206037 33.0 25.0 35.0 2.0 35.0 100-101 23.94870010699178 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.025731741050492474 1101 2 0.03967072828088192 1101 3 0.17583457700651905 1101 4 -0.025113380123940487 1101 5 -0.016554653954941045 1101 6 0.04897844573211074 1101 7 0.09140729004152348 1101 8 0.10584751110008028 1101 9 0.155046715130716 1101 10-11 0.08753009240600562 1101 12-13 0.04129108752545818 1101 14-15 0.11764472446984087 1101 16-17 0.2678180742212888 1101 18-19 0.21255974425539392 1101 20-21 0.29204674646718587 1101 22-23 0.20296257617778934 1101 24-25 0.2092463995502385 1101 26-27 0.21696582209061432 1101 28-29 0.3174797808216283 1101 30-31 0.2301671104738361 1101 32-33 0.14743854202055928 1101 34-35 0.23235687013608697 1101 36-37 0.34231440768898125 1101 38-39 0.29569919806525036 1101 40-41 0.37715874080953427 1101 42-43 0.4128359522224443 1101 44-45 0.410981574859413 1101 46-47 0.31305009885058865 1101 48-49 0.3270538089462818 1101 50-51 0.35375473664384316 1101 52-53 0.4097850930069953 1101 54-55 0.3537668540071479 1101 56-57 0.28460302351655287 1101 58-59 0.22390820812735512 1101 60-61 0.1919926800293439 1101 62-63 0.20548030580454935 1101 64-65 0.3216987268055931 1101 66-67 0.11041590664562762 1101 68-69 0.23668918562258767 1101 70-71 0.08389459154101786 1101 72-73 0.07620909738857051 1101 74-75 0.14527330685390183 1101 76-77 -0.09177315546413212 1101 78-79 -0.04734127511772712 1101 80-81 -0.12023940357648044 1101 82-83 -0.03856016074825064 1101 84-85 -0.27433739640865795 1101 86-87 -0.3230099163047129 1101 88-89 -0.42726917028548783 1101 90-91 -0.4457009403976322 1101 92-93 -0.44656761940852263 1101 94-95 -0.4476693560195777 1101 96-97 -0.606462048748245 1101 98-99 -0.6857208135164825 1101 100-101 -0.35698523579238284 1102 1 0.021342747063286538 1102 2 0.040171663320194284 1102 3 0.04148443031397164 1102 4 0.1254943855717343 1102 5 0.15668863329867833 1102 6 0.10515867510161314 1102 7 0.11833910333962194 1102 8 0.14215317728454124 1102 9 0.18465432193081455 1102 10-11 0.2199359369241236 1102 12-13 0.21192817201650627 1102 14-15 0.2687882205745282 1102 16-17 0.3282311159595679 1102 18-19 0.315789227702858 1102 20-21 0.2583372288591548 1102 22-23 0.25042976897410796 1102 24-25 0.14269005293395054 1102 26-27 0.27528365029178303 1102 28-29 0.36395141204827297 1102 30-31 0.3697990562900557 1102 32-33 0.31683876447436177 1102 34-35 0.2996889662130968 1102 36-37 0.24783246047307017 1102 38-39 0.280044041667459 1102 40-41 0.336493796365815 1102 42-43 0.4666883417870622 1102 44-45 0.47865097902468534 1102 46-47 0.4835760944417231 1102 48-49 0.40097139695097184 1102 50-51 0.514221518992791 1102 52-53 0.5246215429408991 1102 54-55 0.35568069791177237 1102 56-57 0.5228811238825699 1102 58-59 0.4718013632768461 1102 60-61 0.4903929407284764 1102 62-63 0.2644369691079831 1102 64-65 0.24618853600079404 1102 66-67 0.2468941894064045 1102 68-69 0.3065441924298682 1102 70-71 0.32440334124579806 1102 72-73 0.19666435882304256 1102 74-75 0.16663243962317154 1102 76-77 0.23277449487256519 1102 78-79 0.13998674408537326 1102 80-81 0.1379019243725388 1102 82-83 0.19908541302443794 1102 84-85 0.1293820413297091 1102 86-87 0.0745454181901728 1102 88-89 0.1496756148841527 1102 90-91 -0.08488567207111686 1102 92-93 -0.1402919738410553 1102 94-95 -0.16918564951823 1102 96-97 -0.4429869947612062 1102 98-99 -0.5736767659298252 1102 100-101 -0.24715419343350575 1103 1 0.10496659883295223 1103 2 0.1499061171069016 1103 3 0.2091133105250833 1103 4 0.14130291537846063 1103 5 0.2004294290410371 1103 6 0.12501128335449607 1103 7 0.12005761776021018 1103 8 0.11578502346223729 1103 9 0.23079137966929153 1103 10-11 0.1250230744264016 1103 12-13 0.18429511689063105 1103 14-15 0.2158680209409738 1103 16-17 0.2071659924403022 1103 18-19 0.2994547771097942 1103 20-21 0.2530194923480451 1103 22-23 0.2086392198891147 1103 24-25 0.18058732015476409 1103 26-27 0.31513155049766794 1103 28-29 0.2608154355122707 1103 30-31 0.34274568588987364 1103 32-33 0.19769343211148538 1103 34-35 0.25023341785183106 1103 36-37 0.3801506018556111 1103 38-39 0.291095782794919 1103 40-41 0.27087069098128325 1103 42-43 0.3358188005331897 1103 44-45 0.35478749075333127 1103 46-47 0.32336097172086653 1103 48-49 0.20762113988850928 1103 50-51 0.35875507206932156 1103 52-53 0.37137255267275293 1103 54-55 0.27613292540006285 1103 56-57 0.2865526175851727 1103 58-59 0.27226754492195937 1103 60-61 0.26515699751967503 1103 62-63 0.23048990270032021 1103 64-65 0.07535120417568919 1103 66-67 0.0635352039132755 1103 68-69 0.06790647664652028 1103 70-71 0.12941299428746333 1103 72-73 0.16529268169204414 1103 74-75 -0.0044778327275594165 1103 76-77 -9.594345386680914E-4 1103 78-79 -0.05134791254913296 1103 80-81 0.009506168208165633 1103 82-83 -0.025276978848875586 1103 84-85 0.024545643387448024 1103 86-87 -0.060914533276765326 1103 88-89 0.09512034761491961 1103 90-91 -0.012944498963850748 1103 92-93 -0.07958888059236457 1103 94-95 -0.14821938725940598 1103 96-97 -0.11239967877553525 1103 98-99 -6.443458236802257E-4 1103 100-101 0.33966793665385 1104 1 0.1347494949980046 1104 2 0.09609282545304865 1104 3 0.03826296293313192 1104 4 0.1527599246889153 1104 5 0.0840081387663929 1104 6 0.14014966569745013 1104 7 0.1900355017580182 1104 8 0.1765795707201221 1104 9 0.1781841690170154 1104 10-11 0.18526644035289763 1104 12-13 0.257937814683288 1104 14-15 0.20541904282772805 1104 16-17 0.335174551417758 1104 18-19 0.2752276539114433 1104 20-21 0.33757409975594754 1104 22-23 0.31864289663481316 1104 24-25 0.2597352868919174 1104 26-27 0.2008418737049027 1104 28-29 0.2658168541200183 1104 30-31 0.2763953656652802 1104 32-33 0.19575395205963986 1104 34-35 0.2994855154213667 1104 36-37 0.2657369786732673 1104 38-39 0.22426458363111834 1104 40-41 0.24353522369876401 1104 42-43 0.23923316329538125 1104 44-45 0.24056094398832073 1104 46-47 0.2598590386860309 1104 48-49 0.2800035507918821 1104 50-51 0.3820518040889027 1104 52-53 0.3464119973717743 1104 54-55 0.2625513427626629 1104 56-57 0.2043430428158146 1104 58-59 0.31656674476372615 1104 60-61 0.27816168408140385 1104 62-63 0.1428916346974134 1104 64-65 0.20221030086233327 1104 66-67 0.11004987110101183 1104 68-69 0.027188534206835868 1104 70-71 0.12054382032965805 1104 72-73 -0.016559577120176527 1104 74-75 -0.02029474718564117 1104 76-77 -0.05979404232733998 1104 78-79 -0.18739586572159084 1104 80-81 -0.15750408886788136 1104 82-83 -0.16818676941054278 1104 84-85 -0.038070981902059486 1104 86-87 -0.0054597617440066415 1104 88-89 0.03967169077071375 1104 90-91 0.007611497436876391 1104 92-93 0.05549612481632593 1104 94-95 0.1819776947715539 1104 96-97 0.08495622412664972 1104 98-99 0.17747249933019305 1104 100-101 0.518991457751973 1105 1 -0.017107935781709216 1105 2 0.012861079479996818 1105 3 0.04034155258799643 1105 4 0.04757441274144725 1105 5 0.09182787810401294 1105 6 0.036833008980394766 1105 7 -0.03232637465187338 1105 8 0.012153923422630442 1105 9 0.005944857812338 1105 10-11 0.0789705114195769 1105 12-13 0.06938884539327006 1105 14-15 0.14847274567050306 1105 16-17 0.1773985287643285 1105 18-19 0.2719656430496329 1105 20-21 0.20783255649096333 1105 22-23 0.1490523080048476 1105 24-25 0.1534791454241926 1105 26-27 0.2276869224932483 1105 28-29 0.21209680863353242 1105 30-31 0.1742567811349076 1105 32-33 0.2410052767676305 1105 34-35 0.29465465672531366 1105 36-37 0.3207980763714673 1105 38-39 0.30891045207427936 1105 40-41 0.2997688794701645 1105 42-43 0.3055744862599852 1105 44-45 0.2865526457428089 1105 46-47 0.4482826692047652 1105 48-49 0.4311533260009526 1105 50-51 0.40902711142643255 1105 52-53 0.48634266482383737 1105 54-55 0.41098979204866737 1105 56-57 0.3383542268907931 1105 58-59 0.41513185356676985 1105 60-61 0.45821895161694925 1105 62-63 0.4165895775038422 1105 64-65 0.38637244617606115 1105 66-67 0.35226381077991675 1105 68-69 0.4723643782954383 1105 70-71 0.3258177231861765 1105 72-73 0.38491860151860635 1105 74-75 0.3879889701325112 1105 76-77 0.32422400962551734 1105 78-79 0.4156486843531617 1105 80-81 0.49413815941118244 1105 82-83 0.3763775363790778 1105 84-85 0.32815342204538567 1105 86-87 0.3581055517746208 1105 88-89 0.4276737392473642 1105 90-91 0.47818165016791525 1105 92-93 0.49712543639081375 1105 94-95 0.5094254658810335 1105 96-97 0.4972001315265224 1105 98-99 0.5514240913571129 1105 100-101 0.879671272002291 1106 1 0.15225479893334892 1106 2 0.13871164025438532 1106 3 0.14024253010406795 1106 4 0.15322398964538309 1106 5 0.11963810442598799 1106 6 0.14906731629958614 1106 7 0.1602370103284798 1106 8 0.1786095274597841 1106 9 0.1683042670664392 1106 10-11 0.17358397953646687 1106 12-13 0.18992694257297416 1106 14-15 0.3521386295123605 1106 16-17 0.36931469325815414 1106 18-19 0.36328191397938525 1106 20-21 0.3542930322225146 1106 22-23 0.2653337622056213 1106 24-25 0.18878469862627156 1106 26-27 0.18562599718903527 1106 28-29 0.08032601391191463 1106 30-31 0.01831285063069288 1106 32-33 0.22263435548784827 1106 34-35 0.25632203384741103 1106 36-37 0.417192492986068 1106 38-39 0.5292459370726377 1106 40-41 0.5486070862629902 1106 42-43 0.4928360644771601 1106 44-45 0.5686430130261968 1106 46-47 0.6505554867386323 1106 48-49 0.703971521779529 1106 50-51 0.5740433151056266 1106 52-53 0.5299522064609405 1106 54-55 0.4629769270687376 1106 56-57 0.6250409981223797 1106 58-59 0.714552082218681 1106 60-61 0.6855788109732472 1106 62-63 0.6769958079773559 1106 64-65 0.6278549239621967 1106 66-67 0.6665061034416624 1106 68-69 0.6897637407389112 1106 70-71 0.6265454987281487 1106 72-73 0.6580999238659189 1106 74-75 0.4735883474028739 1106 76-77 0.5953960228002728 1106 78-79 0.5283839951354956 1106 80-81 0.5066546410946735 1106 82-83 0.49566792378468705 1106 84-85 0.6278905755990749 1106 86-87 0.6774325430797035 1106 88-89 0.9039698350079313 1106 90-91 0.8676147067325211 1106 92-93 0.9026602372338282 1106 94-95 0.9379894148129679 1106 96-97 1.0310137945111784 1106 98-99 1.1094547088973314 1106 100-101 1.5345037810308106 1107 1 0.11762695871473738 1107 2 0.12990605933566712 1107 3 0.13463270831772434 1107 4 0.09130246146160914 1107 5 0.013974417840294961 1107 6 -0.030203932378427112 1107 7 -0.03082537430559995 1107 8 -0.0018769636426725356 1107 9 -0.05396028792492302 1107 10-11 0.008660802564314452 1107 12-13 0.005653493320842529 1107 14-15 0.0630242456402712 1107 16-17 0.15857030800659544 1107 18-19 0.20332316653303195 1107 20-21 0.15925811863609596 1107 22-23 0.14608070931959105 1107 24-25 0.13053353100821852 1107 26-27 0.09283402698545729 1107 28-29 0.1468129122848012 1107 30-31 0.09127994712094534 1107 32-33 0.08994137827529158 1107 34-35 0.17195136520132337 1107 36-37 0.25810412187073695 1107 38-39 0.1357832844283351 1107 40-41 0.11321364331698192 1107 42-43 0.14791249337233126 1107 44-45 0.3037546511072975 1107 46-47 0.37249980892895707 1107 48-49 0.30986511909416237 1107 50-51 0.29822598246420995 1107 52-53 0.30867955204304565 1107 54-55 0.2536645572146341 1107 56-57 0.373567927237616 1107 58-59 0.4767045064631006 1107 60-61 0.43116288275795256 1107 62-63 0.45906671244615893 1107 64-65 0.45077111164817296 1107 66-67 0.3040633347909747 1107 68-69 0.5165719886220899 1107 70-71 0.5319832159116729 1107 72-73 0.3806830694388239 1107 74-75 0.4428772006738484 1107 76-77 0.3524624932764482 1107 78-79 0.3642047435934508 1107 80-81 0.372335866584546 1107 82-83 0.31891255536916674 1107 84-85 0.5955848337428407 1107 86-87 0.4478436891518456 1107 88-89 0.615239730497084 1107 90-91 0.7578241225622087 1107 92-93 0.749007612638632 1107 94-95 0.9096874040794205 1107 96-97 0.9605100272596623 1107 98-99 1.1336150420708222 1107 100-101 1.633429441038821 1108 1 0.226333604180887 1108 2 0.23506918583010616 1108 3 0.11984801652815236 1108 4 0.10097306303822506 1108 5 0.2393215767786998 1108 6 0.10984707516463743 1108 7 0.1886966760026496 1108 8 0.14566782699202463 1108 9 0.1827082125566264 1108 10-11 0.2672734222253368 1108 12-13 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2316 70-71 -1.306874817268472 2316 72-73 -1.1889768344118323 2316 74-75 -1.0724352553768455 2316 76-77 -1.1141648960344313 2316 78-79 -0.9657825854754485 2316 80-81 -0.848623613871947 2316 82-83 -0.8569988598115508 2316 84-85 -0.9289465383685886 2316 86-87 -0.9823182607869683 2316 88-89 -0.9771232542081272 2316 90-91 -1.2841160054279541 2316 92-93 -1.0674692055407569 2316 94-95 -0.866945698688621 2316 96-97 -0.8985571149947482 2316 98-99 -1.268998738673588 2316 100-101 -1.1038677757686486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18002.0 3 3577.0 4 3175.0 5 3373.0 6 3847.0 7 4443.0 8 5410.0 9 6062.0 10 7304.0 11 8880.0 12 11290.0 13 14775.0 14 16285.0 15 17717.0 16 21746.0 17 27003.0 18 24829.0 19 18250.0 20 18456.0 21 19418.0 22 20725.0 23 22940.0 24 26063.0 25 29805.0 26 34994.0 27 41342.0 28 49807.0 29 61060.0 30 75189.0 31 95768.0 32 124232.0 33 167482.0 34 236286.0 35 351954.0 36 535762.0 37 782132.0 38 665951.0 39 101530.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34697042316419 18.49150667214113 14.346343913119577 23.815178991575102 2 15.425935393164321 19.692057618470333 40.351341170754104 24.530665817611236 3 19.57580137954182 23.59482912920611 29.170125419579534 27.659244071672536 4 13.027436129711933 16.080615930680562 36.59362639864778 34.298321540959726 5 14.370551659010603 36.525541636403524 33.77017637335608 15.33373033122979 6 32.61706075144461 34.961000182534946 17.419854898338933 15.002084167681506 7 29.088030473208526 31.84451381130693 20.53038667407638 18.537069041408166 8 26.163771474082825 34.509236441621105 19.644416145626494 19.68257593866958 9 26.226749692682827 17.049635331480445 20.31896172818729 36.404653247649435 10-11 25.313990076070382 25.91654259370591 26.985974544574244 21.783492785649468 12-13 26.282833173683517 23.306752581020522 27.415472676311776 22.994941568984185 14-15 23.086219769066982 24.74784686870877 26.04201367315659 26.12391968906766 16-17 22.68895247181543 27.88230544899197 26.443373256788423 22.98536882240418 18-19 22.372811837623754 26.61582160715174 28.610961030218835 22.400405525005674 20-21 23.223427475497278 25.74414729272997 28.501404251974442 22.531020979798306 22-23 22.826799320721257 25.725154314320825 28.516119923415562 22.93192644154236 24-25 22.83575554835157 25.83604266303433 28.638565402258298 22.6896363863558 26-27 22.614705404134263 25.980081100752844 28.673472895303426 22.73174059980947 28-29 22.335300263040654 26.358885418923844 28.673258615368947 22.632555702666558 30-31 22.529602020200095 25.93110928994008 28.55081428477356 22.988474405086276 32-33 22.43013161495471 26.016548207350187 28.636465364152237 22.91685481354287 34-35 23.0293108505399 26.1338872176861 28.24226301321273 22.594538918561266 36-37 22.904950396412467 25.903601386266573 28.443179515243404 22.748268702077556 38-39 22.61065683544599 26.178012900134146 28.488239690174094 22.72309057424577 40-41 22.783138096253307 26.14280903239693 28.35033090117322 22.72372197017654 42-43 22.635625216941406 26.188047538274468 27.996220034797947 23.180107209986183 44-45 22.70367373449719 26.14882152033633 28.083307520901247 23.064197224265232 46-47 22.882350814014657 26.459512143866455 27.78345524846875 22.874681793650133 48-49 23.036428908645 26.369961965613598 27.653675394862496 22.93993373087891 50-51 22.642454978392497 26.601545040714097 27.79419200621237 22.96180797468104 52-53 22.911459277998844 26.74286905910735 27.36222162264039 22.983450040253413 54-55 22.689381481425883 26.364751687780704 27.57201129417673 23.373855536616688 56-57 22.787939979602072 26.58937738558569 27.522606568952167 23.100076065860073 58-59 23.09629523129688 26.42940439943074 27.414289890345025 23.060010478927357 60-61 22.909994055759675 26.39158157020947 27.91002882936103 22.788395544669825 62-63 22.674372353950194 26.50590227646077 27.83936059933851 22.980364770250524 64-65 22.506309171731907 26.648966816397884 27.60825848023795 23.236465531632263 66-67 22.621765515512955 26.608807989209616 27.365532767086542 23.40389372819089 68-69 22.488942764472693 26.499129072065873 27.669489674453146 23.342438489008288 70-71 22.72376323069661 26.322810295651756 27.531288316089185 23.422138157562447 72-73 22.67092974989739 26.335622529313817 27.697483249394327 23.295964471394466 74-75 22.477833709141137 26.799154841915033 27.340591064691722 23.382420384252107 76-77 22.668093970605256 26.469942005314724 27.361915986313605 23.50004803776642 78-79 22.685800421212566 26.498311711488494 27.446626670210428 23.369261197088516 80-81 22.75525220762262 26.596471215111784 27.288419251465324 23.359857325800274 82-83 22.77094407612249 26.529497423505173 27.445807363803855 23.253751136568486 84-85 22.71136281850662 26.491554412043055 27.26529491564374 23.53178785380659 86-87 22.60751628408133 26.492265666908065 27.473315639964092 23.426902409046516 88-89 22.728590902831904 26.42174042571252 27.197271802130597 23.65239686932498 90-91 22.67830313857421 26.43217914539416 27.277942704234864 23.611575011796766 92-93 22.828986323852547 26.377640425727655 27.320846071885153 23.472527178534644 94-95 22.726121104636434 26.22091855009916 27.35021360614347 23.70274673912094 96-97 22.574382120389025 26.15388899612292 27.50774064386104 23.763988239627018 98-99 22.639374047031104 26.255379965846686 27.09339197547295 24.011854011649255 100-101 22.842378361205288 25.62560242982132 27.502256190518693 24.0297630184547 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4105.0 1 2701.5 2 1419.0 3 1562.5 4 1362.0 5 1325.5 6 2089.5 7 3898.0 8 5999.5 9 6467.5 10 5127.0 11 3901.0 12 3690.0 13 3852.0 14 4111.0 15 4366.0 16 4532.0 17 4752.0 18 4971.5 19 5302.0 20 5875.5 21 6784.5 22 8077.5 23 9692.0 24 11555.0 25 14052.0 26 17851.0 27 22674.5 28 27359.0 29 32106.0 30 37634.0 31 43794.5 32 50013.0 33 56015.5 34 62437.0 35 68436.5 36 75423.0 37 81955.0 38 85941.5 39 89762.0 40 94233.0 41 100054.0 42 106755.5 43 113554.0 44 119481.0 45 124781.5 46 128141.5 47 135923.5 48 153066.0 49 153997.0 50 140158.5 51 133488.5 52 131552.5 53 131818.5 54 130975.0 55 125123.5 56 115791.0 57 105939.0 58 97575.5 59 87191.5 60 74077.5 61 61350.0 62 50197.0 63 41256.0 64 32145.5 65 24355.5 66 18889.5 67 15172.5 68 11661.0 69 8386.0 70 6521.5 71 5155.0 72 3933.5 73 3165.0 74 2565.5 75 2058.5 76 1514.0 77 997.5 78 709.0 79 538.5 80 410.5 81 311.0 82 251.5 83 189.5 84 140.5 85 110.0 86 84.0 87 55.0 88 39.0 89 34.0 90 33.0 91 24.0 92 17.0 93 14.5 94 9.0 95 6.5 96 5.5 97 6.0 98 4.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24422627863879476 2 0.14650857049300528 3 0.17076801821525528 4 0.14512153928915467 5 0.20976807206470202 6 0.17373245745485758 7 0.21373878884435282 8 0.22124507535930907 9 0.1309792603479327 10-11 0.18217702978418338 12-13 0.18783394136067216 14-15 0.15330774306090048 16-17 0.25677755119912926 18-19 0.20670844440914918 20-21 0.175649824119004 22-23 0.21829423446484256 24-25 0.2081226723032714 26-27 0.3087912213435274 28-29 0.28215206322251407 30-31 0.2599595639609042 32-33 0.16993851916197206 34-35 0.32345023739990936 36-37 0.35683417470827467 38-39 0.34097850427994314 40-41 0.30586757713933244 42-43 0.3354439778096765 44-45 0.34439168890902655 46-47 0.3488111510781584 48-49 0.36776724419745016 50-51 0.360641711346296 52-53 0.3604513345143949 54-55 0.3558142988230904 56-57 0.3353487893937259 58-59 0.3118508489990802 60-61 0.2813089658240951 62-63 0.3429910593600401 64-65 0.37527353071240643 66-67 0.3815015727845984 68-69 0.3850507408650397 70-71 0.3842620368471638 72-73 0.3764429883940844 74-75 0.37254026334011253 76-77 0.35720133002694104 78-79 0.33343142272957954 80-81 0.34337181302384223 82-83 0.3516124101761312 84-85 0.3525642943356366 86-87 0.3490287246003311 88-89 0.35648061773474415 90-91 0.3763342016329981 92-93 0.3759942430046033 94-95 0.38352772620983117 96-97 0.3837181030417322 98-99 0.38137918767837625 100-101 0.3565350111152873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3676918.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.87086964240859 #Duplication Level Percentage of deduplicated Percentage of total 1 72.1720630023241 35.2711148281028 2 14.818886278572396 14.48423719131578 3 5.328947506738708 7.81290896799197 4 2.476998542269556 4.842122914547662 5 1.407378176355129 3.438989769711112 6 0.8423880935741035 2.4700943225626255 7 0.5408393172503818 1.8501901439582875 8 0.40171466045183063 1.5705715843508674 9 0.2883069400065822 1.2680829793857051 >10 1.521249558091662 13.897241831187014 >50 0.13222268122191722 4.460604846279109 >100 0.06515838732999286 5.553841743333261 >500 0.0022300613487453817 0.7700610268695723 >1k 0.001338036798384255 1.1170148862116824 >5k 1.6725459979803183E-4 0.5401025950526667 >10k+ 1.1150306653202122E-4 0.6528203691399835 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13577 0.36924946381725127 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10383 0.2823832350898225 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7422 0.20185383519567202 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6997 0.19029524183025023 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5404 0.14697091422762215 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3805 0.1034834064833646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 2.719669027158071E-5 9 0.0 0.0 0.0 0.0 2.719669027158071E-5 10-11 0.0 0.0 0.0 2.719669027158071E-5 2.719669027158071E-5 12-13 0.0 0.0 0.0 2.719669027158071E-5 1.223851062221132E-4 14-15 0.0 0.0 0.0 9.518841595053249E-5 1.3598345135790356E-4 16-17 0.0 0.0 0.0 1.3598345135790356E-4 1.6318014162948426E-4 18-19 0.0 0.0 0.0 1.3598345135790356E-4 1.6318014162948426E-4 20-21 0.0 0.0 0.0 2.3117186730843602E-4 1.6318014162948426E-4 22-23 1.3598345135790355E-5 0.0 0.0 2.855652478515974E-4 1.9037683190106496E-4 24-25 2.719669027158071E-5 0.0 0.0 3.943520089379203E-4 1.9037683190106496E-4 26-27 2.719669027158071E-5 0.0 0.0 5.575321505674046E-4 2.1757352217264568E-4 28-29 2.719669027158071E-5 0.0 0.0 0.0016046047260232618 2.1757352217264568E-4 30-31 2.719669027158071E-5 0.0 0.0 0.0054121413640445615 2.1757352217264568E-4 32-33 2.719669027158071E-5 0.0 0.0 0.012592067595741867 2.447702124442264E-4 34-35 2.719669027158071E-5 0.0 0.0 0.025143340156076367 2.447702124442264E-4 36-37 2.719669027158071E-5 0.0 0.0 0.04558165289516927 2.447702124442264E-4 38-39 2.719669027158071E-5 0.0 0.0 0.07597395427366072 2.991635929873878E-4 40-41 2.719669027158071E-5 0.0 0.0 0.11063613602479033 2.991635929873878E-4 42-43 2.719669027158071E-5 0.0 0.0 0.14687572581167163 2.991635929873878E-4 44-45 2.719669027158071E-5 0.0 0.0 0.19504106428264106 3.5355697353054924E-4 46-47 2.719669027158071E-5 0.0 0.0 0.2564511909158703 3.943520089379203E-4 48-49 2.719669027158071E-5 0.0 0.0 0.3218864277092935 4.0795035407371064E-4 50-51 2.719669027158071E-5 0.0 0.0 0.3943112139025129 4.0795035407371064E-4 52-53 2.719669027158071E-5 2.719669027158071E-5 0.0 0.4688029485563725 4.21548699209501E-4 54-55 2.719669027158071E-5 2.719669027158071E-5 0.0 0.553099090053137 4.3514704434529137E-4 56-57 2.719669027158071E-5 2.719669027158071E-5 0.0 0.6578743393244015 4.487453894810817E-4 58-59 2.719669027158071E-5 2.719669027158071E-5 0.0 0.7777981450769367 4.759420797526624E-4 60-61 2.719669027158071E-5 2.719669027158071E-5 0.0 0.9049698687868482 4.895404248884528E-4 62-63 2.719669027158071E-5 2.719669027158071E-5 0.0 1.0352012201523122 4.895404248884528E-4 64-65 2.719669027158071E-5 2.719669027158071E-5 0.0 1.177004763228334 4.895404248884528E-4 66-67 2.719669027158071E-5 2.719669027158071E-5 0.0 1.3461273816821588 5.303354602958239E-4 68-69 2.719669027158071E-5 2.719669027158071E-5 0.0 1.5373065159462356 5.439338054316142E-4 70-71 2.719669027158071E-5 2.719669027158071E-5 0.0 1.7502022073921693 5.439338054316142E-4 72-73 2.719669027158071E-5 2.719669027158071E-5 0.0 1.967898114671037 6.255238762463563E-4 74-75 2.719669027158071E-5 2.719669027158071E-5 0.0 2.1929099316329603 7.343106373326792E-4 76-77 2.719669027158071E-5 2.719669027158071E-5 0.0 2.447253379052783 7.615073276042598E-4 78-79 2.719669027158071E-5 2.719669027158071E-5 0.0 2.7359462462856117 7.751056727400502E-4 80-81 2.719669027158071E-5 2.719669027158071E-5 0.0 3.0557657255342656 8.023023630116309E-4 82-83 2.719669027158071E-5 2.719669027158071E-5 0.0 3.3777881366949165 8.159007081474213E-4 84-85 2.719669027158071E-5 2.719669027158071E-5 0.0 3.708078341698129 8.159007081474213E-4 86-87 2.719669027158071E-5 2.719669027158071E-5 0.0 4.05823572894473 8.294990532832116E-4 88-89 2.719669027158071E-5 2.719669027158071E-5 0.0 4.441899438605919 8.566957435547924E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 21005 0.0 24.618002 1 GTACATG 13405 0.0 24.605902 1 TACATGG 13725 0.0 23.36837 2 GGTATCA 15590 0.0 22.986448 1 ACATGGG 13640 0.0 22.400906 3 GAGTACT 11530 0.0 21.759237 12-13 ATCAACG 24210 0.0 21.165234 3 TCAACGC 24350 0.0 21.063028 4 TATCAAC 24670 0.0 20.866743 2 CAACGCA 24610 0.0 20.840502 5 AACGCAG 24985 0.0 20.584675 6 GTACTTT 12230 0.0 20.203575 14-15 AGAGTAC 18525 0.0 19.74285 10-11 AGTACTT 12130 0.0 19.411045 12-13 ACGCAGA 27400 0.0 18.754332 7 CATGGGG 7840 0.0 18.51823 4 CGCAGAG 27545 0.0 18.500574 8 ATATGCG 130 0.007616556 18.24823 5 GCAGAGT 28715 0.0 17.431381 9 GTGTAGC 2335 0.0 16.690664 1 >>END_MODULE