FastQCFastQC Report
Thu 26 May 2016
SRR938688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938688_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3676918
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134130.364789206612712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120210.3269314137546717No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT101930.2772158639382222No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80870.21993963422627322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG129200.022.156351
GAGTACT134950.020.70963912-13
TACATGG134300.020.6724822
GTATCAA253500.020.3149011
ACATGGG131950.020.2493843
GGTATCA200950.019.7588961
AGAGTAC208000.019.45878810-11
AGTACTT141750.019.4316312-13
GTACTTT143500.019.29385814-15
CGTATAC2001.2957502E-518.9833473
TCAACGC296750.017.240144
ATCAACG297400.017.2184183
TATCAAC299100.017.1364212
CAACGCA298750.017.1247255
TACTTTT161400.016.96298814-15
AACGCAG302700.016.9441476
ACTTTTT165950.016.86914316-17
CATGGGG80500.016.684124
TACCTGG27400.015.9349252
GTGTAGC22550.015.8153551
Produced by FastQC (version 0.11.5)