##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938688_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3676918 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71192803320607 31.0 31.0 34.0 30.0 34.0 2 31.95141610446575 31.0 31.0 34.0 30.0 34.0 3 32.0178151919624 33.0 31.0 34.0 30.0 34.0 4 35.718815051083546 37.0 35.0 37.0 33.0 37.0 5 35.606313766039925 37.0 35.0 37.0 33.0 37.0 6 35.64271707990225 37.0 35.0 37.0 33.0 37.0 7 35.55755989119148 37.0 35.0 37.0 33.0 37.0 8 35.53096887121225 37.0 35.0 37.0 33.0 37.0 9 37.12849837826136 39.0 37.0 39.0 33.0 39.0 10-11 37.02026071835162 39.0 37.0 39.0 33.0 39.0 12-13 36.91764882980801 39.0 37.0 39.0 33.0 39.0 14-15 38.00356888568089 40.0 37.0 41.0 33.0 41.0 16-17 37.883241617028176 40.0 37.0 41.0 33.0 41.0 18-19 38.05123475693502 40.0 37.0 41.0 33.0 41.0 20-21 38.051733816201505 40.0 37.0 41.0 33.0 41.0 22-23 37.95657368480885 40.0 37.0 41.0 33.0 41.0 24-25 37.848113148022335 40.0 37.0 41.0 32.0 41.0 26-27 37.62221335912305 40.0 37.0 41.0 32.0 41.0 28-29 37.408892719391616 39.0 36.0 41.0 31.5 41.0 30-31 37.14735574739497 39.0 36.0 41.0 31.0 41.0 32-33 36.96515179288741 39.0 36.0 40.5 31.0 41.0 34-35 36.919150767028256 39.0 35.5 41.0 30.5 41.0 36-37 36.782633580623774 39.0 35.0 41.0 30.0 41.0 38-39 36.62718668188956 39.0 35.0 40.5 30.0 41.0 40-41 36.4147738676794 39.0 35.0 40.0 30.0 41.0 42-43 36.169732776200064 39.0 35.0 40.0 30.0 41.0 44-45 35.88066473062494 38.0 35.0 40.0 29.0 41.0 46-47 35.528047538726724 38.0 34.5 40.0 27.5 41.0 48-49 35.168357439573036 38.0 34.0 40.0 26.5 41.0 50-51 34.88695872467104 38.0 34.0 40.0 26.0 41.0 52-53 34.60777218855574 38.0 33.5 40.0 25.0 41.0 54-55 34.26009201184253 38.0 33.0 40.0 24.0 41.0 56-57 33.91192555830726 37.0 33.0 40.0 23.5 41.0 58-59 33.52668539249447 37.0 32.5 39.5 23.0 41.0 60-61 33.068842574134095 36.0 31.5 39.0 22.0 40.0 62-63 32.63161974240383 36.0 31.0 39.0 20.5 40.0 64-65 32.153303935524264 35.0 31.0 38.5 19.5 40.0 66-67 31.67051400112812 35.0 30.0 38.0 18.0 40.0 68-69 31.21474017098015 34.5 30.0 37.5 15.5 40.0 70-71 30.6863046714667 34.0 29.0 37.0 12.5 39.0 72-73 30.106721172460198 34.0 29.0 36.0 8.0 39.0 74-75 29.52772036254276 34.0 28.0 36.0 7.0 38.5 76-77 27.78932750200032 31.5 26.0 34.0 4.5 36.0 78-79 28.76684712577218 33.0 27.5 35.0 2.0 37.0 80-81 28.784513687822248 33.0 28.0 35.0 2.0 37.0 82-83 28.356070491645447 33.0 27.0 35.0 2.0 36.0 84-85 27.838422967278575 33.0 26.5 35.0 2.0 36.0 86-87 27.078091624561658 32.5 25.5 35.0 2.0 35.0 88-89 26.307100675076246 32.0 24.5 34.0 2.0 35.0 90-91 25.59809139067012 31.0 22.0 34.0 2.0 35.0 92-93 25.09520310216328 31.0 20.0 34.0 2.0 35.0 94-95 24.493632302923263 31.0 18.0 34.0 2.0 35.0 96-97 23.534310256579015 31.0 4.5 34.0 2.0 35.0 98-99 22.2748050133291 30.0 2.0 34.0 2.0 35.0 100-101 18.443219293984797 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08455544772987622 1101 2 0.16017669151813152 1101 3 0.037810046129344244 1101 4 0.030798628473213796 1101 5 -0.11613676980378784 1101 6 0.08115345528616302 1101 7 0.16446713129736423 1101 8 0.16930953221223888 1101 9 0.26884203854679356 1101 10-11 0.24672911927714836 1101 12-13 0.18949986548287256 1101 14-15 0.326609556486666 1101 16-17 0.33047804017578386 1101 18-19 0.3294467459248622 1101 20-21 0.22428405821912634 1101 22-23 0.1912658385211543 1101 24-25 0.20816433696513315 1101 26-27 0.10126710785966964 1101 28-29 0.13365657343929627 1101 30-31 0.21145358155153104 1101 32-33 0.3323223690684429 1101 34-35 0.4976513407790719 1101 36-37 0.39768658157560566 1101 38-39 0.3612148161817572 1101 40-41 0.4232093754370112 1101 42-43 0.5936521378580792 1101 44-45 0.6302400699408679 1101 46-47 0.5408387694735737 1101 48-49 0.5477206560949455 1101 50-51 0.46823153951186214 1101 52-53 0.4134139758556543 1101 54-55 0.5184021803429104 1101 56-57 0.5209740658562652 1101 58-59 0.5850971963280358 1101 60-61 0.7046706560838203 1101 62-63 0.4407349687709541 1101 64-65 0.5280399481224194 1101 66-67 0.49196140473125993 1101 68-69 0.52289078734686 1101 70-71 0.7006197856686391 1101 72-73 0.5538256127835552 1101 74-75 0.29587228689701917 1101 76-77 0.3560598963720665 1101 78-79 0.1666348201940444 1101 80-81 0.5006819040104986 1101 82-83 0.6516991739514637 1101 84-85 0.7566670799684374 1101 86-87 0.801015872372755 1101 88-89 0.8445325005736848 1101 90-91 0.7476234986222821 1101 92-93 0.6935251865524599 1101 94-95 0.8577713230546458 1101 96-97 0.832009646056445 1101 98-99 0.6417222648294079 1101 100-101 1.1403774468779027 1102 1 0.261482904213981 1102 2 0.15651677061894986 1102 3 0.17414717600222218 1102 4 0.15265536460050555 1102 5 -0.10459633837274396 1102 6 -0.04728522423251036 1102 7 0.06384897627600594 1102 8 0.11157701535767472 1102 9 0.1706075142063952 1102 10-11 0.17388033068829145 1102 12-13 0.21307781913662893 1102 14-15 0.29652723438760376 1102 16-17 0.3702645247473413 1102 18-19 0.30499703518972154 1102 20-21 0.2851018302351349 1102 22-23 0.24096998916519397 1102 24-25 0.3083613004432024 1102 26-27 0.2608205363962881 1102 28-29 0.20977504250611645 1102 30-31 0.15845786778908888 1102 32-33 0.27419409184732046 1102 34-35 0.36169902995252556 1102 36-37 0.2965167277319338 1102 38-39 0.3679273654226307 1102 40-41 0.45913012400468034 1102 42-43 0.43817233040954306 1102 44-45 0.366015072237559 1102 46-47 0.43447523849071246 1102 48-49 0.46418032481147975 1102 50-51 0.48570758787762713 1102 52-53 0.33146614880184444 1102 54-55 0.34694400398301894 1102 56-57 0.4365446290917703 1102 58-59 0.4108750059112083 1102 60-61 0.1846467586633338 1102 62-63 0.2669282528111694 1102 64-65 0.35428882589781807 1102 66-67 0.4734659407463404 1102 68-69 0.5435649540421146 1102 70-71 0.6215990082001106 1102 72-73 0.561433378873506 1102 74-75 0.520613509888225 1102 76-77 0.35674446816427974 1102 78-79 0.4189638428443523 1102 80-81 0.5712335082051858 1102 82-83 0.485700913522809 1102 84-85 0.6219689795787673 1102 86-87 0.842702795267396 1102 88-89 0.6445384739431042 1102 90-91 0.7692610929197059 1102 92-93 0.9984153258403943 1102 94-95 1.1093550545140332 1102 96-97 1.2250753921539435 1102 98-99 1.4914327831246759 1102 100-101 2.058655937582902 1103 1 0.2406707867106057 1103 2 0.2354379839764107 1103 3 0.20715372760504636 1103 4 0.08860841187620139 1103 5 0.11238461876187245 1103 6 0.11113849690687516 1103 7 0.09338115402589864 1103 8 0.0783758833659789 1103 9 0.16797726639519794 1103 10-11 0.25184518980488235 1103 12-13 0.253940924170891 1103 14-15 0.2437182327459695 1103 16-17 0.19614475955217614 1103 18-19 0.03259080078769472 1103 20-21 0.2284184767870059 1103 22-23 0.22542010575890004 1103 24-25 0.23631878070056445 1103 26-27 0.28184474781129154 1103 28-29 0.21429668638717203 1103 30-31 0.32269904032973784 1103 32-33 0.49141643941141666 1103 34-35 0.27968101368864495 1103 36-37 0.293085043090727 1103 38-39 0.2755102443057993 1103 40-41 0.3228805187028243 1103 42-43 0.31926104824969315 1103 44-45 0.40624273258029575 1103 46-47 0.4118535620035928 1103 48-49 0.4269948020044936 1103 50-51 0.42553350603693474 1103 52-53 0.5396246688473596 1103 54-55 0.5392226523717554 1103 56-57 0.36801002932470794 1103 58-59 0.48206580183385483 1103 60-61 0.5860274681361375 1103 62-63 0.5627377690413198 1103 64-65 0.555071448568448 1103 66-67 0.5786869279218116 1103 68-69 0.5270785478839457 1103 70-71 0.661711993601557 1103 72-73 0.8174224241650201 1103 74-75 0.8324328326609027 1103 76-77 0.7930130164014138 1103 78-79 0.8004561443633413 1103 80-81 0.9998164530216194 1103 82-83 0.972145446908474 1103 84-85 0.9673178772827953 1103 86-87 1.0325438445952635 1103 88-89 0.9232704382340629 1103 90-91 0.9405061292525367 1103 92-93 0.9584249994861977 1103 94-95 1.2055261219684006 1103 96-97 1.4609370858043143 1103 98-99 1.6504861540399673 1103 100-101 1.9419042916980942 1104 1 0.05307220801542201 1104 2 0.13384378881882242 1104 3 0.08226587429834353 1104 4 0.10786525179987194 1104 5 -0.00833004223846956 1104 6 0.042421693055246124 1104 7 0.08096906341586418 1104 8 -0.06085689707611408 1104 9 0.012772126196296085 1104 10-11 0.08215871533730024 1104 12-13 0.08141875462386139 1104 14-15 0.026085064083950726 1104 16-17 0.21326810200884694 1104 18-19 0.17429027964436017 1104 20-21 0.14623246835785864 1104 22-23 0.15976100975974106 1104 24-25 0.1435168466344905 1104 26-27 0.21558664370055425 1104 28-29 0.0770351823773936 1104 30-31 -0.013890832899882355 1104 32-33 0.11058653008333152 1104 34-35 0.16598687450855465 1104 36-37 0.2797775968147036 1104 38-39 0.14354082145043634 1104 40-41 0.2520526929961733 1104 42-43 0.2826034573232832 1104 44-45 0.347199366019197 1104 46-47 0.2887858366386524 1104 48-49 0.4200969375313761 1104 50-51 0.37798770404496196 1104 52-53 0.2636837141309556 1104 54-55 0.3376983828093145 1104 56-57 0.21645509377286487 1104 58-59 0.48488569788018765 1104 60-61 0.6547249694033681 1104 62-63 0.5537606460412832 1104 64-65 0.37954002594418057 1104 66-67 0.414714580328269 1104 68-69 0.414438581010355 1104 70-71 0.37803179326717284 1104 72-73 0.4765028556544628 1104 74-75 0.35968262819893937 1104 76-77 0.3781710609385591 1104 78-79 0.4012090244808064 1104 80-81 0.5350599313261775 1104 82-83 0.5835208961893485 1104 84-85 0.5928920300117184 1104 86-87 0.7345356068868476 1104 88-89 0.657587815557541 1104 90-91 0.590722873477695 1104 92-93 0.7747781622146555 1104 94-95 0.7662326507634916 1104 96-97 0.798980866709929 1104 98-99 1.0588615123607177 1104 100-101 1.5650367666569558 1105 1 0.09505937564975397 1105 2 0.11510121801364193 1105 3 0.023002625897760254 1105 4 0.02962902849874638 1105 5 0.061659180476247855 1105 6 0.002724038171386667 1105 7 0.06287861082271462 1105 8 0.15607334641656934 1105 9 0.19191464522824475 1105 10-11 0.06930521773841747 1105 12-13 0.1303069838736235 1105 14-15 0.06030885520537055 1105 16-17 0.15104902462898906 1105 18-19 0.14232780335174766 1105 20-21 0.047144113108956276 1105 22-23 0.040290939179087104 1105 24-25 0.06481307933932356 1105 26-27 0.16818389020982494 1105 28-29 0.08688989374162048 1105 30-31 0.10093107114001754 1105 32-33 0.18304901430041554 1105 34-35 0.14785006373971754 1105 36-37 0.07732708515781184 1105 38-39 0.2161638423129304 1105 40-41 0.17440839491970195 1105 42-43 0.2641610920261428 1105 44-45 0.25441532643908715 1105 46-47 0.21957228790049044 1105 48-49 0.2404025118708759 1105 50-51 0.2651233102936743 1105 52-53 0.35935820577591926 1105 54-55 0.39643026217768096 1105 56-57 0.42333264611401944 1105 58-59 0.4726098284332636 1105 60-61 0.4687646947939115 1105 62-63 0.5742544926868476 1105 64-65 0.4002693285375969 1105 66-67 0.39679291904144165 1105 68-69 0.34927008026809503 1105 70-71 0.45612346094641154 1105 72-73 0.6280856373563246 1105 74-75 0.6792557829850594 1105 76-77 0.8101560559461412 1105 78-79 0.5660735225710063 1105 80-81 0.6678825763044358 1105 82-83 0.7311150587573003 1105 84-85 0.686768821603323 1105 86-87 0.8304997710925228 1105 88-89 0.701087765369202 1105 90-91 0.7251559697811913 1105 92-93 0.9521158491662156 1105 94-95 1.2197370514883161 1105 96-97 1.263892647497837 1105 98-99 1.4597554261001697 1105 100-101 2.2525938228731768 1106 1 0.0989607026569459 1106 2 0.05722363522131246 1106 3 0.043822367909776005 1106 4 0.10640481405946645 1106 5 0.029633614671304542 1106 6 0.12132596778599947 1106 7 0.05986650042588337 1106 8 0.09669506993732568 1106 9 0.053308055732038895 1106 10-11 0.11585108050491044 1106 12-13 0.1475574816337044 1106 14-15 0.12162528499472103 1106 16-17 0.21385800655077247 1106 18-19 -0.05572530606015391 1106 20-21 0.015128476029005355 1106 22-23 0.10449331836636588 1106 24-25 0.11675116645792372 1106 26-27 0.21257482250386062 1106 28-29 0.27403636494037187 1106 30-31 0.19591435116712574 1106 32-33 0.20286032881065097 1106 34-35 0.2713905411657649 1106 36-37 0.13615809031545467 1106 38-39 0.263934578786035 1106 40-41 0.4215225117132775 1106 42-43 0.20467187859296843 1106 44-45 0.34968673806550044 1106 46-47 0.4472739601625406 1106 48-49 0.40619759092999885 1106 50-51 0.5056511742674914 1106 52-53 0.41053548779407123 1106 54-55 0.5101140971543288 1106 56-57 0.5884557490656306 1106 58-59 0.6682476762034 1106 60-61 0.6909538065569336 1106 62-63 0.7581824683919223 1106 64-65 0.7038199729780459 1106 66-67 0.7803942396942496 1106 68-69 0.6741056605822138 1106 70-71 0.7214704897423623 1106 72-73 0.7727553682481592 1106 74-75 0.9233870460247822 1106 76-77 0.9680620348919469 1106 78-79 0.7913326622933212 1106 80-81 0.6933302956101137 1106 82-83 0.9449675496758267 1106 84-85 0.9531535345440254 1106 86-87 0.9655868923447635 1106 88-89 1.1597310514329813 1106 90-91 1.0566648447375044 1106 92-93 1.1520145099797858 1106 94-95 1.4397746747368885 1106 96-97 1.4753818371020628 1106 98-99 1.7368377444971692 1106 100-101 1.9978861510135673 1107 1 -0.10522630016110313 1107 2 -0.13015689331060543 1107 3 -0.09091517257093784 1107 4 -0.06561767777870386 1107 5 -0.1242653428477638 1107 6 -0.02178032322843393 1107 7 -0.06648258288719688 1107 8 -0.03363146898656311 1107 9 -0.06333222594144416 1107 10-11 -0.1639568455032503 1107 12-13 -7.759940253180275E-4 1107 14-15 -0.05497243034951538 1107 16-17 -0.0838254786331234 1107 18-19 -0.09175724394484774 1107 20-21 -0.14389584852611392 1107 22-23 -0.01714752166175515 1107 24-25 -0.07034854254077771 1107 26-27 -0.08034458393232313 1107 28-29 -0.09419851186031991 1107 30-31 -0.15686067133128034 1107 32-33 -0.16563566088935033 1107 34-35 -0.25642208815902023 1107 36-37 -0.19809105408819505 1107 38-39 -0.1833846621144275 1107 40-41 -0.4359952406794321 1107 42-43 -0.44748139960557154 1107 44-45 -0.23796836956557144 1107 46-47 -0.21016726480181802 1107 48-49 -0.15772908725409707 1107 50-51 -0.41155797223819235 1107 52-53 -0.37467369812487306 1107 54-55 -0.3042346314393427 1107 56-57 -0.37731218561344804 1107 58-59 -0.08838360610727136 1107 60-61 -0.0884928311628741 1107 62-63 0.021763623594800663 1107 64-65 -0.034388891065887606 1107 66-67 -0.2506979019570359 1107 68-69 -0.36338292599231536 1107 70-71 -0.3272705847946966 1107 72-73 -0.22801647491330712 1107 74-75 -0.11833682462087225 1107 76-77 -0.06459842637466551 1107 78-79 -0.002613131325670537 1107 80-81 0.10631401136162566 1107 82-83 0.060473057680002995 1107 84-85 0.04126189475501718 1107 86-87 0.09934238556896346 1107 88-89 0.03922005841569742 1107 90-91 0.3153406903163223 1107 92-93 0.5034741146647086 1107 94-95 0.696571821216299 1107 96-97 0.9396996919692739 1107 98-99 1.1740441977628855 1107 100-101 1.691446351495724 1108 1 0.09826085544485963 1108 2 0.0019070558866403076 1108 3 0.033467907627986904 1108 4 0.011351119725674153 1108 5 -0.03736106507217585 1108 6 -0.0283150706992501 1108 7 0.014349258842877077 1108 8 0.01599895012419239 1108 9 -0.04086444250246757 1108 10-11 -0.034524361902199985 1108 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-1.0302448375067002 2316 54-55 -0.852838855879817 2316 56-57 -0.9469446910279302 2316 58-59 -0.6386117422491537 2316 60-61 -0.8428635582376955 2316 62-63 -0.8558688718976128 2316 64-65 -0.7948800303965946 2316 66-67 -0.808756315723631 2316 68-69 -0.7670840751255703 2316 70-71 -0.7882917053433793 2316 72-73 -0.6952647075262597 2316 74-75 -1.0362391206699577 2316 76-77 -1.0440186679484427 2316 78-79 -0.9859519506101719 2316 80-81 -0.9423266315763179 2316 82-83 -0.947340495247726 2316 84-85 -1.3365465295161911 2316 86-87 -1.4569376103629885 2316 88-89 -1.8210517881915464 2316 90-91 -2.3496498735720586 2316 92-93 -1.9563662046935697 2316 94-95 -1.6927350389387001 2316 96-97 -1.9211848798848727 2316 98-99 -1.920385809154748 2316 100-101 -1.6404419554906369 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 22.0 4 65.0 5 178.0 6 408.0 7 977.0 8 2181.0 9 3901.0 10 6040.0 11 8736.0 12 11826.0 13 15425.0 14 19665.0 15 25040.0 16 30392.0 17 38368.0 18 37728.0 19 31276.0 20 31839.0 21 34081.0 22 36830.0 23 40857.0 24 47623.0 25 55601.0 26 66355.0 27 79495.0 28 94492.0 29 112562.0 30 133514.0 31 160229.0 32 193640.0 33 239293.0 34 306929.0 35 409254.0 36 538058.0 37 588296.0 38 265549.0 39 10183.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.390810054510894 20.68369998239474 15.56408505248057 22.361404910613793 2 17.12727887866958 21.718243376654033 37.777807391951626 23.376670352724755 3 20.77117303132678 24.461219967374852 29.657800364326864 25.109806636971506 4 14.79543465478425 17.91027159158839 35.35866179229453 31.935631961332835 5 14.344860529504222 37.1939910525179 33.682243061838605 14.778905356139274 6 32.596987110813394 36.21882923833559 16.583401395179454 14.600782255671572 7 28.863765795157793 32.37896520944987 20.284488258916845 18.472780736475496 8 26.316061440586925 35.39233129485074 19.07711839100029 19.214488873562043 9 26.058481587024783 17.090644936873762 19.964328821039796 36.88654465506166 10-11 25.58539515975064 26.521124485234644 26.59904300286272 21.29443735215199 12-13 26.452398448918363 23.67913834358014 27.2486631466897 22.619800060811798 14-15 23.005449128862814 25.324538105010774 25.82453565730865 25.845477108817764 16-17 22.562651897398407 28.37711296488491 26.377751559498087 22.682483578218594 18-19 22.358943006071932 26.965939952971485 28.666902552626954 22.008214488329628 20-21 23.22576951675289 25.927203163083867 28.7208335894355 22.126193730727746 22-23 22.882506490490133 25.965428111260575 28.678257170815343 22.473808227433953 24-25 22.953437030217692 26.159219614879643 28.748953772910756 22.13838958199191 26-27 22.758239649723862 26.188276144074152 28.800728771927105 22.25275543427488 28-29 22.389716061114225 26.61526582860972 28.794495825036076 22.200522285239977 30-31 22.696630710829016 26.11771597843629 28.659613839634172 22.526039471100525 32-33 22.634799035496574 26.235328065515738 28.688605511463678 22.441267387524007 34-35 23.103188250885136 26.261357833547862 28.521290476996715 22.114163438570294 36-37 23.076528667955795 26.06901830520019 28.605380771937234 22.24907225490679 38-39 22.72310247432865 26.366731475175115 28.6887809835081 22.221385066988134 40-41 22.863319666474055 26.263230264570613 28.52145108509622 22.351998983859108 42-43 22.814005703569425 26.388079349515657 28.082898947273943 22.71501599964098 44-45 23.03662664489587 26.29808524581425 28.010407446385642 22.654880662904237 46-47 23.14241313456412 26.665780955338935 27.76673736039168 22.42506854970527 48-49 23.277726012509607 26.629191694856523 27.594772596355472 22.498309696278397 50-51 22.888781552588757 26.76235280807263 27.709572992808923 22.63929264652969 52-53 23.180228658893125 26.96377781609489 27.22702817957866 22.628965345433322 54-55 22.968366441677514 26.588735457249797 27.395226110563247 23.047671990509443 56-57 23.03939859913916 26.79631818480985 27.392393881555076 22.771889334495913 58-59 23.40656068078996 26.58095599423972 27.26322817695861 22.74925514801171 60-61 23.09886187503009 26.67617574562049 27.765318397307247 22.459643982042174 62-63 22.883889854681456 26.714017397320777 27.703876212710316 22.69821653528745 64-65 22.758331837491774 26.808717220671983 27.467567435257255 22.96538350657898 66-67 22.893643976406125 26.6932973084412 27.300863786905545 23.112194928247128 68-69 22.72835997958611 26.644530316704916 27.461521003310253 23.165588700398718 70-71 22.982942770517468 26.535838730066175 27.347986736710872 23.133231762705485 72-73 22.934506562289396 26.518948750012918 27.456513852090257 23.09003083560743 74-75 22.69522564558703 27.024110355841348 27.04645251285354 23.234211485718085 76-77 22.842157393625563 26.639501059866827 27.224699931156277 23.293641615351333 78-79 22.838647939471617 26.65384667230463 27.283876367616223 23.223629020607532 80-81 22.87237897865923 26.694298770765723 27.21114966279178 23.222172587783266 82-83 22.847702021603077 26.64870580632949 27.29528589969115 23.208306272376284 84-85 22.64925407637592 26.63817904016353 27.238573174598947 23.473993708861606 86-87 22.702638768035015 26.485284455875824 27.260305426943443 23.551771349145717 88-89 22.63214475247714 26.559468378710594 27.061362641080596 23.74702422773167 90-91 22.70335583805336 26.436750236910946 27.175309185157364 23.684584739878332 92-93 22.726985924563667 26.472421620157405 27.176370216013375 23.624222239265556 94-95 22.52810544936967 26.312776361356537 27.25756385488201 23.901554334391786 96-97 22.370951956877985 26.215737076361968 27.383346176979046 24.029964789781 98-99 22.394965022336642 26.318998683136257 26.997039368297038 24.288996926230066 100-101 22.63056442669687 25.42393025594442 27.610815985596453 24.334689331762252 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2112.0 1 1406.5 2 867.0 3 1061.5 4 895.5 5 806.0 6 1566.5 7 3561.5 8 5370.0 9 5763.0 10 4810.0 11 3532.0 12 3170.0 13 3323.5 14 3631.0 15 3919.5 16 4248.0 17 4573.0 18 4904.5 19 5422.5 20 6090.0 21 7068.5 22 8357.5 23 10050.5 24 12107.0 25 14834.5 26 18591.5 27 23458.0 28 28379.0 29 32911.0 30 38589.0 31 45532.0 32 51961.0 33 57664.5 34 64203.5 35 70039.5 36 76413.0 37 83068.0 38 87182.5 39 90576.5 40 94994.0 41 101263.0 42 108059.5 43 114418.0 44 119929.5 45 125021.0 46 128645.5 47 137064.0 48 153453.5 49 153549.0 50 140578.0 51 133814.0 52 131304.0 53 131303.5 54 129219.0 55 123056.5 56 114690.0 57 105305.0 58 96431.0 59 85869.5 60 73087.0 61 60617.0 62 50016.0 63 41052.5 64 32209.0 65 24461.5 66 18449.0 67 14637.5 68 11015.0 69 7815.5 70 5982.5 71 4567.5 72 3425.5 73 2793.0 74 2292.0 75 1798.0 76 1290.5 77 811.5 78 546.0 79 374.0 80 260.0 81 165.0 82 107.0 83 79.0 84 50.5 85 33.0 86 21.0 87 12.5 88 7.5 89 3.5 90 3.0 91 2.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20560697845315015 2 0.0 3 0.0 4 0.0 5 0.021757352217264568 6 0.0031276193812317813 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0025564888855285866 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.21548699209501E-4 26-27 0.0012918427879000836 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.005860886753525643 36-37 0.014441442534209356 38-39 0.012129723861124996 40-41 0.0071391311962899365 42-43 0.007424696444141534 44-45 0.0034675780096265407 46-47 6.52720566517937E-4 48-49 0.0017405881773811655 50-51 2.719669027158071E-5 52-53 0.0 54-55 0.0 56-57 9.790808497769055E-4 58-59 3.5355697353054924E-4 60-61 0.0022029319119980374 62-63 8.159007081474212E-4 64-65 0.0015774080357516813 66-67 0.0054665347445877225 68-69 2.5836855758001675E-4 70-71 5.439338054316142E-5 72-73 0.0 74-75 3.399586283947589E-4 76-77 0.052435218843607606 78-79 0.1302585480557358 80-81 9.654825046411152E-4 82-83 9.246874692337442E-4 84-85 0.0 86-87 0.09259113202959653 88-89 0.3237222043026252 90-91 0.35611346241607783 92-93 4.3514704434529137E-4 94-95 1.7677848676527462E-4 96-97 6.799172567895178E-5 98-99 0.0 100-101 0.0012374494073569223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3676918.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.60713286698033 #Duplication Level Percentage of deduplicated Percentage of total 1 71.65483558870795 34.82936114021958 2 15.199532834309688 14.776114239866423 3 5.453964871927334 7.953047855448457 4 2.543898958662644 4.946065387355522 5 1.388352970268362 3.374192864605053 6 0.799062229477917 2.3304074374331183 7 0.5410409541420208 1.8408914681121313 8 0.3945909626821362 1.534394828096022 9 0.2918630966281932 1.2767965485097417 >10 1.5255826252511877 13.786336083000108 >50 0.1379955842560992 4.673332238003585 >100 0.06518103804219297 5.437652225435335 >500 0.0025824813733182775 0.8421225397540375 >1k 0.0012912406752225733 1.2063964712100979 >5k 5.614089892272057E-5 0.22068194853256265 >10k+ 1.6842269676816172E-4 0.9722067244181537 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13413 0.364789206612712 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12021 0.3269314137546717 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10193 0.2772158639382222 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8087 0.21993963422627322 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.719669027158071E-5 2 0.0 0.0 0.0 0.0 2.719669027158071E-5 3 0.0 0.0 0.0 0.0 2.719669027158071E-5 4 0.0 0.0 0.0 2.719669027158071E-5 5.439338054316142E-5 5 0.0 0.0 0.0 2.719669027158071E-5 5.439338054316142E-5 6 0.0 0.0 0.0 2.719669027158071E-5 5.439338054316142E-5 7 0.0 0.0 0.0 2.719669027158071E-5 5.439338054316142E-5 8 0.0 0.0 0.0 2.719669027158071E-5 5.439338054316142E-5 9 0.0 0.0 0.0 2.719669027158071E-5 8.159007081474213E-5 10-11 0.0 0.0 0.0 5.439338054316142E-5 1.0878676108632284E-4 12-13 0.0 0.0 0.0 5.439338054316142E-5 1.6318014162948426E-4 14-15 0.0 0.0 0.0 1.0878676108632284E-4 1.6318014162948426E-4 16-17 0.0 0.0 0.0 1.3598345135790356E-4 1.6318014162948426E-4 18-19 0.0 0.0 0.0 1.3598345135790356E-4 1.6318014162948426E-4 20-21 0.0 0.0 0.0 2.3117186730843602E-4 1.6318014162948426E-4 22-23 0.0 0.0 0.0 2.855652478515974E-4 1.9037683190106496E-4 24-25 0.0 0.0 0.0 3.671553186663396E-4 1.9037683190106496E-4 26-27 0.0 0.0 0.0 5.303354602958239E-4 1.9037683190106496E-4 28-29 0.0 0.0 0.0 0.0015230146552085199 1.9037683190106496E-4 30-31 0.0 0.0 0.0 0.005303354602958239 1.9037683190106496E-4 32-33 0.0 0.0 0.0 0.012551272560334496 2.991635929873878E-4 34-35 0.0 0.0 0.0 0.025143340156076367 2.991635929873878E-4 36-37 0.0 0.0 0.0 0.04537767771813242 2.991635929873878E-4 38-39 0.0 0.0 0.0 0.0754436188133649 3.263602832589685E-4 40-41 0.0 0.0 0.0 0.10987462869718606 3.263602832589685E-4 42-43 0.0 0.0 0.0 0.14581505489108 3.263602832589685E-4 44-45 0.0 0.0 0.0 0.193422861211482 3.263602832589685E-4 46-47 0.0 0.0 0.0 0.25442503749063755 3.263602832589685E-4 48-49 0.0 0.0 0.0 0.3194387255848512 3.263602832589685E-4 50-51 0.0 0.0 0.0 0.39168673329130543 3.399586283947589E-4 52-53 0.0 0.0 0.0 0.46533537054674595 3.5355697353054924E-4 54-55 0.0 0.0 0.0 0.5481764891139809 3.807536638021299E-4 56-57 0.0 0.0 0.0 0.6516462972522096 3.807536638021299E-4 58-59 0.0 0.0 0.0 0.7697071297211415 3.807536638021299E-4 60-61 0.0 0.0 0.0 0.8946351264836474 3.807536638021299E-4 62-63 0.0 2.719669027158071E-5 0.0 1.022568357521163 3.807536638021299E-4 64-65 0.0 2.719669027158071E-5 0.0 1.1620057885435573 3.807536638021299E-4 66-67 0.0 2.719669027158071E-5 0.0 1.3282863528640019 3.807536638021299E-4 68-69 0.0 2.719669027158071E-5 0.0 1.5149916315784036 3.807536638021299E-4 70-71 0.0 2.719669027158071E-5 0.0 1.7239709996252297 4.0795035407371064E-4 72-73 0.0 2.719669027158071E-5 0.0 1.9367986993454844 4.351470443452913E-4 74-75 0.0 2.719669027158071E-5 0.0 2.1588188803775337 4.6234373461687204E-4 76-77 0.0 2.719669027158071E-5 0.0 2.409558766336372 4.6234373461687204E-4 78-79 0.0 2.719669027158071E-5 0.0 2.692567525302441 4.6234373461687204E-4 80-81 0.0 2.719669027158071E-5 0.0 3.0062133558594453 4.6234373461687204E-4 82-83 0.0 2.719669027158071E-5 0.0 3.3205119069829676 5.847288408389853E-4 84-85 0.0 2.719669027158071E-5 0.0 3.6407121398954234 5.983271859747756E-4 86-87 0.0 2.719669027158071E-5 0.0 3.979650892404998 5.983271859747756E-4 88-89 0.0 2.719669027158071E-5 0.0 4.3483292257265465 5.983271859747756E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12920 0.0 22.15635 1 GAGTACT 13495 0.0 20.709639 12-13 TACATGG 13430 0.0 20.672482 2 GTATCAA 25350 0.0 20.314901 1 ACATGGG 13195 0.0 20.249384 3 GGTATCA 20095 0.0 19.758896 1 AGAGTAC 20800 0.0 19.458788 10-11 AGTACTT 14175 0.0 19.43163 12-13 GTACTTT 14350 0.0 19.293858 14-15 CGTATAC 200 1.2957502E-5 18.983347 3 TCAACGC 29675 0.0 17.24014 4 ATCAACG 29740 0.0 17.218418 3 TATCAAC 29910 0.0 17.136421 2 CAACGCA 29875 0.0 17.124725 5 TACTTTT 16140 0.0 16.962988 14-15 AACGCAG 30270 0.0 16.944147 6 ACTTTTT 16595 0.0 16.869143 16-17 CATGGGG 8050 0.0 16.68412 4 TACCTGG 2740 0.0 15.934925 2 GTGTAGC 2255 0.0 15.815355 1 >>END_MODULE