FastQCFastQC Report
Thu 26 May 2016
SRR938687_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938687_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270644
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250011.101053269468926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181530.7994648214339193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137760.6067001256031329No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91560.40323362006549684No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73640.3243132785236259No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49390.217515383300949No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44960.1980054997613012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43570.19188388844750653No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39750.17506046742686215No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36260.15969037858862947No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35640.15695987570046205No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32520.14321928052129704No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31230.13753807289914227No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30030.13225322859946342No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.1267922228231286No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT25460.11212677989151977No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22820.10050012243222628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG180300.026.3733141
GTATCAA283350.025.7833251
TACATGG180150.025.7786252
ACATGGG183100.023.9398423
GGTATCA205250.023.7217521
TACCTGG17450.023.0829562
GAGTACT158650.022.98430812-13
TATCAAC331300.021.941782
ATCAACG334100.021.7720763
CATGGGA131300.021.7269974
TCAACGC335850.021.6586284
GTACTTT168750.021.56608214-15
CAACGCA338750.021.4312445
AGTACTT161900.021.29439512-13
AACGCAG345050.021.0658236
ATGGGAG48450.020.93095
ATGGGAT48650.019.481175
ACTTTTT188200.019.47717716-17
CATGGGG52900.019.3493334
ACGCAGA374850.019.2660687
Produced by FastQC (version 0.11.5)