##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938687_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2270644 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.975761061619522 33.0 31.0 34.0 30.0 34.0 2 32.03239873797918 33.0 31.0 34.0 30.0 34.0 3 32.036999635345744 34.0 31.0 34.0 30.0 34.0 4 35.61277725614407 37.0 35.0 37.0 33.0 37.0 5 35.65133592055822 37.0 35.0 37.0 33.0 37.0 6 35.76455490160501 37.0 35.0 37.0 35.0 37.0 7 35.70230075696586 37.0 35.0 37.0 33.0 37.0 8 35.684197522817314 37.0 35.0 37.0 33.0 37.0 9 37.297387877624146 39.0 37.0 39.0 34.0 39.0 10-11 37.209808318697256 39.0 37.0 39.0 34.0 39.0 12-13 37.10108013409412 39.0 37.0 39.0 33.0 39.0 14-15 38.267064321839975 40.0 38.0 41.0 33.0 41.0 16-17 38.22799655075829 40.0 38.0 41.0 33.0 41.0 18-19 38.227085795923976 40.0 38.0 41.0 33.5 41.0 20-21 38.15386427815193 40.0 38.0 41.0 33.5 41.0 22-23 38.067398940564885 40.0 38.0 41.0 33.0 41.0 24-25 38.06716486600277 40.0 38.0 41.0 33.0 41.0 26-27 37.96384263671452 40.0 38.0 41.0 33.0 41.0 28-29 37.83886729932125 40.0 37.5 41.0 33.0 41.0 30-31 37.58365247920854 40.0 37.0 41.0 33.0 41.0 32-33 37.31540038861222 40.0 36.0 41.0 32.5 41.0 34-35 36.9784358534407 39.0 35.5 41.0 31.5 41.0 36-37 36.71264341746218 39.0 35.0 41.0 31.0 41.0 38-39 36.52675430406528 39.0 35.0 41.0 30.5 41.0 40-41 36.32450551473502 39.0 35.0 41.0 30.0 41.0 42-43 36.02477402886582 39.0 35.0 41.0 30.0 41.0 44-45 35.86318418915515 39.0 35.0 41.0 29.5 41.0 46-47 35.56015297862633 39.0 35.0 41.0 27.5 41.0 48-49 35.24629003930163 39.0 35.0 41.0 26.0 41.0 50-51 34.07691011889138 38.0 34.0 40.0 23.5 40.5 52-53 34.18591972145347 38.0 33.5 39.5 23.5 40.5 54-55 34.698564151844145 38.0 35.0 40.0 24.0 41.0 56-57 34.601709691171315 38.0 35.0 40.0 23.5 41.0 58-59 34.3957370684264 38.0 34.5 40.0 23.0 41.0 60-61 34.113042599368285 38.0 34.0 40.0 22.5 41.0 62-63 33.77196381290946 37.0 34.0 40.0 22.0 41.0 64-65 33.388517090305655 37.0 33.5 40.0 20.5 41.0 66-67 32.994412598364164 36.0 33.0 39.0 19.5 41.0 68-69 32.605606603236794 35.5 33.0 39.0 17.5 41.0 70-71 32.200222051541324 35.0 32.5 38.5 14.0 40.0 72-73 31.79592419595498 35.0 32.0 37.5 10.0 40.0 74-75 31.35982611100639 35.0 31.5 37.0 7.0 39.0 76-77 30.94511909396629 35.0 31.0 36.5 4.0 39.0 78-79 30.539566968666158 35.0 31.0 36.0 2.0 39.0 80-81 30.120300672408355 35.0 31.0 36.0 2.0 37.5 82-83 29.68527408964153 34.0 30.0 35.0 2.0 37.0 84-85 29.29825679410775 34.0 30.0 35.0 2.0 36.5 86-87 28.952865794902237 34.0 29.0 35.0 2.0 36.0 88-89 28.60254447636882 34.0 29.0 35.0 2.0 36.0 90-91 28.211019428849262 34.0 29.0 35.0 2.0 35.5 92-93 27.79797669735987 34.0 28.0 35.0 2.0 35.0 94-95 27.332068126927865 34.0 27.0 35.0 2.0 35.0 96-97 26.798591280711552 34.0 25.5 35.0 2.0 35.0 98-99 26.176557619776595 33.0 24.5 35.0 2.0 35.0 100-101 24.022438127685362 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20444321451552483 1101 2 0.14538503432049765 1101 3 0.2838279869617608 1101 4 0.17358970849364397 1101 5 0.013603637517952905 1101 6 -0.04290523598684359 1101 7 0.19628357188871348 1101 8 0.20186861937921208 1101 9 0.31546715357290367 1101 10-11 0.20695495406639708 1101 12-13 0.23745810060936634 1101 14-15 0.34269152154443105 1101 16-17 0.3023524431387088 1101 18-19 0.38857639603961047 1101 20-21 0.325920148950992 1101 22-23 0.27972622207645514 1101 24-25 0.21134956981994435 1101 26-27 0.2994651199690068 1101 28-29 0.08295971063495955 1101 30-31 0.07996465016555732 1101 32-33 0.0619855943261598 1101 34-35 0.0653384578658418 1101 36-37 0.1653313313204805 1101 38-39 0.19355017523601958 1101 40-41 0.20217851132755982 1101 42-43 0.15667003258364076 1101 44-45 0.20119954061369327 1101 46-47 0.22274765805810404 1101 48-49 0.18851490697765882 1101 50-51 0.05932219127033278 1101 52-53 0.11552810052319984 1101 54-55 0.10876952608320778 1101 56-57 0.23866092388136906 1101 58-59 0.14216232547960317 1101 60-61 0.16904367547505927 1101 62-63 0.12564149136388636 1101 64-65 0.10408286262811117 1101 66-67 0.033905193822860724 1101 68-69 0.031217665185870658 1101 70-71 0.021278151642668774 1101 72-73 -0.043371095772354096 1101 74-75 0.013855638671756765 1101 76-77 0.04674042864236938 1101 78-79 -0.13142108425331855 1101 80-81 -0.26545828044204 1101 82-83 -0.3080675807784097 1101 84-85 -0.15590906315375008 1101 86-87 -0.1648353083289784 1101 88-89 -0.44314930226432736 1101 90-91 -0.31109915411633793 1101 92-93 -0.6469420395478167 1101 94-95 -0.6537964907517768 1101 96-97 -0.84364568557341 1101 98-99 -0.9285619880480738 1101 100-101 -0.6828520979029626 1102 1 0.17923834498337143 1102 2 0.21153905263521722 1102 3 0.2118852345649742 1102 4 0.24471169536416681 1102 5 0.2377035237318026 1102 6 0.2593161191675506 1102 7 0.23243845176956057 1102 8 0.2119069541204297 1102 9 0.30437236050227057 1102 10-11 0.1785927912779286 1102 12-13 0.14824094732288273 1102 14-15 0.10567022303553841 1102 16-17 0.1873554487171063 1102 18-19 0.31737231281034184 1102 20-21 0.38983053022867153 1102 22-23 0.41464906239259847 1102 24-25 0.3638478640180409 1102 26-27 0.31744016227982996 1102 28-29 0.34957540955819866 1102 30-31 0.34520798317869605 1102 32-33 0.30440752603664833 1102 34-35 0.3593959292781861 1102 36-37 0.23664939875832403 1102 38-39 0.40071761318593957 1102 40-41 0.3017713965738835 1102 42-43 0.3682579979902769 1102 44-45 0.35387589181181767 1102 46-47 0.2621480674505605 1102 48-49 0.24776282906831426 1102 50-51 0.12041772707038945 1102 52-53 0.01662308175195193 1102 54-55 0.0127755114920447 1102 56-57 0.013894264708014248 1102 58-59 -0.017468745962048615 1102 60-61 0.0025065078093433613 1102 62-63 0.019539523527036806 1102 64-65 0.006406318320337334 1102 66-67 0.05409832327861608 1102 68-69 0.001842815588581459 1102 70-71 -0.15350083683908622 1102 72-73 -0.15314858394067699 1102 74-75 -0.14685664874670223 1102 76-77 -0.11855727216443412 1102 78-79 -0.19885031946978415 1102 80-81 -0.17148250926607744 1102 82-83 -0.28035892085044267 1102 84-85 -0.19697853410263377 1102 86-87 -0.31442438089760927 1102 88-89 -0.29233571645580625 1102 90-91 -0.21656286980347517 1102 92-93 -0.4189206410180226 1102 94-95 -0.5270691249357498 1102 96-97 -0.5958390645862544 1102 98-99 -0.7042533453514324 1102 100-101 -0.4116653450268686 1103 1 0.11358978867024305 1103 2 0.09644824330791124 1103 3 0.13907541234957677 1103 4 0.1276766695381113 1103 5 0.14212334460723497 1103 6 0.17672027031135684 1103 7 0.14850068469409194 1103 8 0.11005731742620384 1103 9 0.06379850462936787 1103 10-11 0.17732364180173477 1103 12-13 0.17197588130773767 1103 14-15 0.3199873754303013 1103 16-17 0.28584080254044864 1103 18-19 0.3459587897566365 1103 20-21 0.2585210938636635 1103 22-23 0.39438844466030076 1103 24-25 0.26314550871683196 1103 26-27 0.2675656981423984 1103 28-29 0.22990818962739468 1103 30-31 0.15339495581314821 1103 32-33 0.22162721232903237 1103 34-35 0.25762972233913217 1103 36-37 0.372296008703195 1103 38-39 0.2880735550374496 1103 40-41 0.4199854912143266 1103 42-43 0.3628804465606734 1103 44-45 0.5304337933334082 1103 46-47 0.5552835493527581 1103 48-49 0.5779865049265993 1103 50-51 0.7307373911604813 1103 52-53 0.6804579945125937 1103 54-55 0.6830124109176197 1103 56-57 0.6846863192219743 1103 58-59 0.6091587455604852 1103 60-61 0.5371745542131094 1103 62-63 0.5676552140916513 1103 64-65 0.35106960150127264 1103 66-67 0.47157045060044567 1103 68-69 0.4637626584706922 1103 70-71 0.3054216967259791 1103 72-73 0.2020702749006773 1103 74-75 0.34167396058392896 1103 76-77 0.3634338332260256 1103 78-79 0.3723426477833023 1103 80-81 0.37513584704059255 1103 82-83 0.4810679724817284 1103 84-85 0.4017903635193747 1103 86-87 0.35697065654797555 1103 88-89 0.3500898300677697 1103 90-91 0.3004750902460884 1103 92-93 0.3973695810733524 1103 94-95 0.4323185808633525 1103 96-97 0.35282845561343024 1103 98-99 0.3284640292618626 1103 100-101 0.42650860943593827 1104 1 0.09293316818537178 1104 2 0.1296652895661765 1104 3 0.20532962453096815 1104 4 0.18923148328199346 1104 5 0.1538586113020486 1104 6 0.10910500966652847 1104 7 0.15623080021636326 1104 8 0.053922608182723764 1104 9 0.14587488051648023 1104 10-11 0.1466031015999576 1104 12-13 0.1491754071029021 1104 14-15 0.25232433098623375 1104 16-17 0.2861284926044547 1104 18-19 0.2306289374006809 1104 20-21 0.3115795729408717 1104 22-23 0.22323408284848512 1104 24-25 0.14507499878471464 1104 26-27 0.2096691093922871 1104 28-29 0.3014553461161711 1104 30-31 0.2826946585663208 1104 32-33 0.25244253031441843 1104 34-35 0.3112119473976733 1104 36-37 0.42626711885511526 1104 38-39 0.21982555396014192 1104 40-41 0.19731308548959703 1104 42-43 0.2880024433390176 1104 44-45 0.4712889021916098 1104 46-47 0.4608648353214235 1104 48-49 0.38464515760512086 1104 50-51 0.4725705080187197 1104 52-53 0.3718271741200496 1104 54-55 0.3399924590094443 1104 56-57 0.16666965491816654 1104 58-59 0.2796494654332946 1104 60-61 0.33418206416774154 1104 62-63 0.38099342979720063 1104 64-65 0.4097790084727251 1104 66-67 0.31217070594758667 1104 68-69 0.4807253148392263 1104 70-71 0.3572823228359212 1104 72-73 0.2689677912883468 1104 74-75 0.06571136384641818 1104 76-77 -0.110412336112649 1104 78-79 0.10315715620845367 1104 80-81 0.09387260530077413 1104 82-83 0.201950460272176 1104 84-85 0.20606940274118557 1104 86-87 0.376503567935881 1104 88-89 0.22057263204657218 1104 90-91 0.3021395263439466 1104 92-93 0.19805456775904773 1104 94-95 0.1955110559812212 1104 96-97 0.3135829499561851 1104 98-99 0.509355426163971 1104 100-101 0.6043952544169215 1105 1 0.11519884569562677 1105 2 0.15698988795298874 1105 3 0.20832740866607224 1105 4 0.23645195454651002 1105 5 0.18123485144329976 1105 6 0.17330714258925184 1105 7 0.2068773668084063 1105 8 0.20595179405243869 1105 9 0.18687291458190458 1105 10-11 0.2182216409109614 1105 12-13 0.14570584991714242 1105 14-15 0.2381269877376866 1105 16-17 0.24152617944806565 1105 18-19 0.22405397624040546 1105 20-21 0.32206097658741584 1105 22-23 0.3354294408460774 1105 24-25 0.2673897753828811 1105 26-27 0.2400496381644288 1105 28-29 0.19279537868595753 1105 30-31 0.0831559455813391 1105 32-33 0.1563593997045345 1105 34-35 0.17484933240114486 1105 36-37 0.17596298440049196 1105 38-39 0.25534019952227993 1105 40-41 0.16085923015667447 1105 42-43 0.024244547768113023 1105 44-45 0.1772322768107486 1105 46-47 0.07796379472036108 1105 48-49 -0.09562946614921941 1105 50-51 0.0641838673858075 1105 52-53 0.16393777262432963 1105 54-55 0.1334577665912846 1105 56-57 0.01114105031385293 1105 58-59 0.004806036531178393 1105 60-61 -0.04587601900859539 1105 62-63 0.03998764946860689 1105 64-65 0.11992556856026937 1105 66-67 0.028432280500744866 1105 68-69 0.059627805351532004 1105 70-71 -0.09574220857428628 1105 72-73 0.10431870095194284 1105 74-75 0.08813462755011159 1105 76-77 0.19183394223167838 1105 78-79 0.05589470138020758 1105 80-81 0.11513649190651876 1105 82-83 3.7189798650416606E-4 1105 84-85 -0.039979214619826564 1105 86-87 0.03148934280069682 1105 88-89 -0.08222051670887964 1105 90-91 0.01292490676036806 1105 92-93 0.07074906893715038 1105 94-95 0.1454287051224128 1105 96-97 0.15596147455520182 1105 98-99 0.17673610907295512 1105 100-101 0.38960596263036607 1106 1 0.10426076012464236 1106 2 0.11564554605698163 1106 3 0.1490496870671123 1106 4 0.12269582534529633 1106 5 0.025791025325823114 1106 6 0.06682605646446405 1106 7 0.035063025091048416 1106 8 0.09995560609805665 1106 9 0.16365944532788745 1106 10-11 0.19815461110072619 1106 12-13 0.1474352873390714 1106 14-15 0.212510622287013 1106 16-17 0.17586802244982636 1106 18-19 0.11349110762209591 1106 20-21 0.2209399783960606 1106 22-23 0.0891612426864512 1106 24-25 0.02848996810536164 1106 26-27 -0.01155430486258524 1106 28-29 0.169363901570307 1106 30-31 0.11352725782769113 1106 32-33 0.19792646102335 1106 34-35 0.20678959451706902 1106 36-37 0.2179196329335369 1106 38-39 0.28496374774795896 1106 40-41 0.23601510921325541 1106 42-43 0.2620123043170324 1106 44-45 0.3600030595561563 1106 46-47 0.31719125181423635 1106 48-49 0.37031428998194116 1106 50-51 0.4268846559637325 1106 52-53 0.38714749226091527 1106 54-55 0.21499260868429104 1106 56-57 0.34617888905792427 1106 58-59 0.3460780559632255 1106 60-61 0.3669325170838249 1106 62-63 0.22837667443654652 1106 64-65 0.2532401734379519 1106 66-67 0.25186501403270967 1106 68-69 0.5055555735851271 1106 70-71 0.4114223091296978 1106 72-73 0.22367658191159734 1106 74-75 0.10169489322719372 1106 76-77 0.11141426777306762 1106 78-79 0.31590917372560057 1106 80-81 0.2664649656571356 1106 82-83 0.18145348789496651 1106 84-85 0.2669496464996435 1106 86-87 0.45171358170197706 1106 88-89 0.45247983683489323 1106 90-91 0.44789319108267023 1106 92-93 0.704784248201257 1106 94-95 0.46805707700514887 1106 96-97 0.4872508737585157 1106 98-99 0.5569279795489983 1106 100-101 0.7374027090225361 1107 1 -0.03020141850516822 1107 2 0.006744768328736939 1107 3 0.07556298770614234 1107 4 0.011511399724845717 1107 5 0.009014684116131377 1107 6 2.503394188408947E-4 1107 7 -0.1539158226822721 1107 8 0.04147975530298709 1107 9 0.061294755180760774 1107 10-11 0.007718191622835491 1107 12-13 0.247721114851835 1107 14-15 0.19075226294683745 1107 16-17 0.13565798628793146 1107 18-19 0.25994536035899785 1107 20-21 0.1895117324952338 1107 22-23 0.1719695846887319 1107 24-25 0.037494575030606825 1107 26-27 0.07432264565012048 1107 28-29 -0.006223555704181649 1107 30-31 0.12553023003752628 1107 32-33 0.196493261439457 1107 34-35 0.12817444615426155 1107 36-37 0.20144368306480942 1107 38-39 0.05459985019012237 1107 40-41 0.02560633128686618 1107 42-43 0.35579335152562663 1107 44-45 0.25771723204339736 1107 46-47 0.3226595234742149 1107 48-49 0.3575189266292824 1107 50-51 0.3006409347570127 1107 52-53 0.2584353507837349 1107 54-55 0.23258531686281714 1107 56-57 0.20524878552595283 1107 58-59 0.15313041639321057 1107 60-61 0.12210049647649868 1107 62-63 0.1475065104696327 1107 64-65 -0.04651065630395834 1107 66-67 0.11465215427147513 1107 68-69 0.21764424451196618 1107 70-71 0.32750335138461395 1107 72-73 0.08072527661611417 1107 74-75 0.0687176306324524 1107 76-77 0.1348179434059027 1107 78-79 0.08568774409266666 1107 80-81 0.06994641411406377 1107 82-83 0.25587167781917586 1107 84-85 1.1237498827654235E-4 1107 86-87 0.20539922097477614 1107 88-89 0.10144600317957142 1107 90-91 0.23630286067200856 1107 92-93 0.2807960379669474 1107 94-95 0.23581385116673914 1107 96-97 0.16971107091510262 1107 98-99 0.20304845312109876 1107 100-101 0.473475330016246 1108 1 0.057474922743733003 1108 2 0.0705501040074239 1108 3 0.009666769588285717 1108 4 0.05942696700716965 1108 5 0.04820905526828767 1108 6 0.04410610019280625 1108 7 0.09221375808583332 1108 8 0.11373305345602347 1108 9 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-1.5282430707108077 2316 54-55 -1.37439089360457 2316 56-57 -1.400216089928847 2316 58-59 -1.2672345430246494 2316 60-61 -1.0045868810773726 2316 62-63 -1.2202645502636429 2316 64-65 -1.461559634753442 2316 66-67 -1.4814816268648272 2316 68-69 -1.4801268162213148 2316 70-71 -1.387585369578165 2316 72-73 -1.2982750032607413 2316 74-75 -1.2421421510636002 2316 76-77 -1.1515100722672038 2316 78-79 -1.069316454060143 2316 80-81 -1.0311646969970454 2316 82-83 -1.043910018234559 2316 84-85 -1.098131117874864 2316 86-87 -1.204146818767736 2316 88-89 -1.154982768161041 2316 90-91 -1.3465697953593363 2316 92-93 -1.4669089718287438 2316 94-95 -1.4736527035491847 2316 96-97 -1.6624176724378223 2316 98-99 -1.8038325992158732 2316 100-101 -2.0224502227405416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5966.0 3 1608.0 4 1505.0 5 1746.0 6 1933.0 7 2391.0 8 3095.0 9 3780.0 10 4704.0 11 6429.0 12 9021.0 13 12696.0 14 14724.0 15 15324.0 16 20979.0 17 28388.0 18 23014.0 19 14077.0 20 13354.0 21 13674.0 22 14535.0 23 15803.0 24 17916.0 25 20370.0 26 23883.0 27 28271.0 28 33649.0 29 40464.0 30 49502.0 31 62155.0 32 79617.0 33 104382.0 34 145637.0 35 225780.0 36 304202.0 37 434856.0 38 400233.0 39 70951.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.70353730340656 18.916636048587254 14.571324099244038 20.80850254876214 2 15.146908242350884 21.266861818491172 40.547089230167046 23.0391407089909 3 19.615575499582597 27.182370898371556 29.894516646288732 23.30753695575711 4 12.26361779281682 16.985487108909723 37.70085158869474 33.05004350957872 5 12.74598893581636 38.99099618657351 33.119624486175056 15.14339039143507 6 29.671410728879156 36.524491652550495 18.05666000691674 15.74743761165361 7 27.20661446851105 35.06625218300018 20.514254043947254 17.21287930454152 8 26.507645232991806 33.57725529416689 19.830859821607557 20.084239651233744 9 27.724797231000913 17.026515827415153 20.70338780306305 34.54529913852089 10-11 24.79160571978415 27.387986972934296 26.381790657591896 21.438616649689656 12-13 26.881987156200577 25.361998770297962 27.244376955004846 20.511637118496616 14-15 23.409372684167348 25.74626536875766 27.65195607176625 23.19240587530874 16-17 20.78865750801692 29.59419631537753 27.791691568668615 21.825454607936933 18-19 20.552373483252996 27.3888820351947 31.7088242291797 20.349920252372602 20-21 22.17103577541225 25.91724186246015 31.47245349874944 20.439268863378157 22-23 21.431083198637825 26.354945719326885 31.421097810970355 20.792873271064938 24-25 21.207199742614492 26.47507459331767 31.79993565362257 20.517790010445268 26-27 20.916777769207883 26.460482387033508 32.03118216776667 20.591557675991933 28-29 20.401913938855756 27.11006470505929 31.937306713879803 20.55071464220515 30-31 20.625496135677285 26.789751277256755 31.758896402574322 20.825856184491638 32-33 20.433562914934377 27.028585200446237 31.590902682192212 20.946949202427174 34-35 21.140748052143056 26.828236390395027 31.39948704869725 20.631528508764667 36-37 21.1654860903142 26.51455198775203 31.650136533400808 20.669825388532963 38-39 20.981979969881852 26.8729752619859 31.602895942010395 20.542148826121856 40-41 20.853001986172842 26.97931939534036 31.566264420976676 20.60141419751012 42-43 20.671916226420603 27.23780231464496 30.89816389250278 21.192117566431655 44-45 20.809391480124603 27.211030742810255 30.71846017235259 21.26111760471255 46-47 21.164040471863586 27.446627392619494 30.190812420754693 21.198519714762227 48-49 21.242740076547026 27.55297031706873 29.86072847305647 21.343561133327775 50-51 20.961804848260456 27.987808020333826 29.933583746209358 21.116803385196356 52-53 21.22593265656049 28.12733905116141 29.490491618787086 21.15623667349102 54-55 20.664879041122614 27.870459369928348 29.786745591130643 21.6779159978184 56-57 21.119795432704322 28.148678357110917 29.261910795462338 21.469615414722423 58-59 21.403437793621844 27.65611009782789 29.386127231526004 21.554324877024264 60-61 21.039966335205172 27.711461412789795 30.095756361656083 21.152815890348954 62-63 21.146507407290287 27.731148743094746 29.93972489324095 21.182618956374018 64-65 20.891403168372637 28.064451405831804 29.783202753860145 21.260942671935414 66-67 20.84992478541722 28.03183346606495 29.413436863994335 21.704804884523494 68-69 20.801115056582482 27.976747530393038 29.598269892366062 21.623867520658415 70-71 21.0151120578102 27.995569943847755 29.264653598767975 21.724664399574074 72-73 20.981047646562523 27.788246151858626 29.45874941986429 21.771956781714564 74-75 20.726495064783155 28.524717227625178 29.10729445886921 21.641493248722455 76-77 20.84929293411828 27.965201490720155 29.27669198307757 21.908813592083995 78-79 20.856588442824158 27.942371107989057 29.393905624607807 21.807134824578974 80-81 20.90835920432333 28.25240356975438 29.113752752938677 21.72548447298361 82-83 20.816795446202292 28.141903696201187 29.09275664869503 21.948544208901488 84-85 20.759396816902218 28.060154665734927 29.050687636692533 22.12976088067032 86-87 20.855482866457002 28.040092534844323 29.058639313154366 22.045785285544305 88-89 20.91243727129237 27.85028542004346 28.838550108647183 22.39872720001699 90-91 20.916574980855472 27.74133939587788 29.051019780688424 22.291065842578227 92-93 21.115259364649184 27.886571736103832 29.02155336979608 21.976615529450903 94-95 20.8396415320893 27.643758356243413 29.085146746916095 22.431453364751185 96-97 20.710955672282154 27.405716358793814 29.21501547936251 22.668312489561522 98-99 20.929133255633857 27.549345397765947 28.728972090787614 22.792549255812585 100-101 21.187871715835097 26.982630056723195 29.242777341952138 22.586720885489573 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10404.0 1 6433.5 2 3430.5 3 4302.5 4 3361.0 5 3021.0 6 5349.5 7 10706.0 8 15820.5 9 15932.5 10 11460.0 11 7530.0 12 6625.5 13 6689.5 14 6703.0 15 6612.5 16 6544.5 17 6572.0 18 6682.0 19 6823.0 20 7143.5 21 7815.5 22 8803.0 23 9809.5 24 10807.0 25 12654.0 26 15397.5 27 18334.5 28 21102.0 29 23926.0 30 27617.5 31 32020.0 32 36930.5 33 40754.5 34 44246.5 35 47480.5 36 51052.5 37 56086.5 38 58576.5 39 58695.0 40 59208.0 41 61464.5 42 64183.5 43 66864.5 44 70086.5 45 73012.0 46 75185.0 47 81390.0 48 100877.5 49 101060.5 50 82922.5 51 75064.0 52 69188.0 53 66873.5 54 65021.0 55 60739.0 56 55967.5 57 50795.0 58 46820.5 59 42340.5 60 35905.5 61 30257.0 62 25243.0 63 19247.5 64 13818.0 65 10437.5 66 7750.0 67 5883.0 68 4295.0 69 2891.0 70 2224.5 71 1841.0 72 1495.0 73 1294.0 74 1064.5 75 861.5 76 637.5 77 395.5 78 283.0 79 194.5 80 126.0 81 94.5 82 76.0 83 54.0 84 37.0 85 25.5 86 17.0 87 12.5 88 12.0 89 13.0 90 8.0 91 6.5 92 7.0 93 5.0 94 3.0 95 4.5 96 4.0 97 1.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.152599879153227 2 0.08733205205219312 3 0.0814746829533824 4 0.09591992403917127 5 0.11472516167219521 6 0.03474785127038849 7 0.03664158714444008 8 0.040913502953347156 9 0.041530068121643025 10-11 0.039988655200903354 12-13 0.043533904918604586 14-15 0.04148602775247903 16-17 0.0402308772313053 18-19 0.043335723257366635 20-21 0.04644056928342796 22-23 0.05848561024977936 24-25 0.07372357798052007 26-27 0.07570539459289963 28-29 0.06612661429973171 30-31 0.05414763388712629 32-33 0.0443926921173024 34-35 0.2773001844410661 36-37 0.4415047008690045 38-39 0.42054148514694506 40-41 0.3967156454292262 42-43 0.4268612781219777 44-45 0.43474450420233196 46-47 0.43439218124902007 48-49 0.4493659067647769 50-51 0.43941278333371503 52-53 0.44533621298627174 54-55 0.43674834099929355 56-57 0.4175467400437938 58-59 0.3972000894900301 60-61 0.3672966788276806 62-63 0.42254532194390665 64-65 0.457953778751755 66-67 0.4599576155487166 68-69 0.4698666986106144 70-71 0.4680390232903088 72-73 0.457887718198009 74-75 0.45172206651505037 76-77 0.4341279390340362 78-79 0.41014795802424336 80-81 0.41503643900144627 82-83 0.42604653129244385 84-85 0.42450511837170424 86-87 0.434854605125242 88-89 0.4455123744629277 90-91 0.4495200480568508 92-93 0.45163398577672237 94-95 0.4561701438006134 96-97 0.4569848906301472 98-99 0.4503347948863846 100-101 0.43725480524467947 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2270644.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.71358642092196 #Duplication Level Percentage of deduplicated Percentage of total 1 70.98685325324354 33.160505041946976 2 14.853037746758899 13.876773247928758 3 5.576715190751956 7.815251010241794 4 2.8290588005854143 5.286219310840663 5 1.628399866714623 3.803419895079567 6 0.9983387416516436 2.7981589851299127 7 0.6077171107022983 1.9872052039185368 8 0.46278464355856264 1.7294664352918785 9 0.32354064267889693 1.3602369395215157 >10 1.5670680580585201 12.930534264804905 >50 0.10586495748317196 3.4183572203907344 >100 0.053815425230773505 4.631085043483889 >500 0.004064433208543282 1.3386754396040135 >1k 0.0022685208605822834 2.6209656118297477 >5k 1.8904340504852362E-4 0.7294319632646681 >10k+ 2.835651075727854E-4 2.513714386722612 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25001 1.101053269468926 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18153 0.7994648214339193 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13776 0.6067001256031329 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9156 0.40323362006549684 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7364 0.3243132785236259 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4939 0.217515383300949 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4496 0.1980054997613012 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4357 0.19188388844750653 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3975 0.17506046742686215 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3626 0.15969037858862947 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3564 0.15695987570046205 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3252 0.14321928052129704 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3123 0.13753807289914227 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3003 0.13225322859946342 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2879 0.1267922228231286 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2546 0.11212677989151977 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2282 0.10050012243222628 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.808073832798096E-5 0.0 12-13 0.0 0.0 0.0 8.808073832798096E-5 0.0 14-15 0.0 0.0 0.0 8.808073832798096E-5 0.0 16-17 0.0 0.0 0.0 8.808073832798096E-5 0.0 18-19 0.0 0.0 0.0 1.101009229099762E-4 0.0 20-21 0.0 0.0 0.0 1.7616147665596192E-4 0.0 22-23 0.0 0.0 0.0 6.826257220418525E-4 0.0 24-25 0.0 0.0 0.0 0.0015414129207396668 0.0 26-27 0.0 0.0 0.0 0.002576361596093443 0.0 28-29 0.0 0.0 0.0 0.00596747002172071 0.0 30-31 0.0 0.0 0.0 0.015017765884920754 0.0 32-33 0.0 0.0 0.0 0.032215530043459034 0.0 34-35 0.0 0.0 0.0 0.05839752951145137 0.0 36-37 0.0 0.0 0.0 0.10369304919661558 0.0 38-39 0.0 0.0 0.0 0.1669570395006879 0.0 40-41 0.0 0.0 0.0 0.23792809440845858 0.0 42-43 0.0 0.0 0.0 0.31198197515770854 0.0 44-45 0.0 0.0 0.0 0.4017802878830852 0.0 46-47 0.0 0.0 0.0 0.5115949483934954 0.0 48-49 0.0 0.0 0.0 0.6398405033990358 0.0 50-51 0.0 0.0 0.0 0.7794925140180495 0.0 52-53 0.0 0.0 0.0 0.9143000840290243 0.0 54-55 0.0 0.0 0.0 1.059192898578553 0.0 56-57 0.0 0.0 0.0 1.2420705315320235 0.0 58-59 0.0 0.0 0.0 1.4454049159621676 0.0 60-61 0.0 0.0 0.0 1.6807786689591147 0.0 62-63 0.0 0.0 0.0 1.9124089905771227 0.0 64-65 0.0 0.0 0.0 2.13895264955669 0.0 66-67 0.0 0.0 0.0 2.4039875911855844 0.0 68-69 0.0 0.0 0.0 2.715991586527875 0.0 70-71 0.0 0.0 0.0 3.062963634986374 0.0 72-73 0.0 0.0 0.0 3.417114263618603 0.0 74-75 0.0 0.0 0.0 3.767147117734 0.0 76-77 0.0 0.0 0.0 4.13521450302205 0.0 78-79 0.0 0.0 0.0 4.570619612761842 0.0 80-81 0.0 0.0 0.0 5.0458151960413 0.0 82-83 0.0 0.0 0.0 5.520724516921191 0.0 84-85 0.0 0.0 0.0 5.997901916813028 0.0 86-87 0.0 0.0 0.0 6.494104756183708 0.0 88-89 0.0 0.0 0.0 7.027103323990903 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18030 0.0 26.373314 1 GTATCAA 28335 0.0 25.783325 1 TACATGG 18015 0.0 25.778625 2 ACATGGG 18310 0.0 23.939842 3 GGTATCA 20525 0.0 23.721752 1 TACCTGG 1745 0.0 23.082956 2 GAGTACT 15865 0.0 22.984308 12-13 TATCAAC 33130 0.0 21.94178 2 ATCAACG 33410 0.0 21.772076 3 CATGGGA 13130 0.0 21.726997 4 TCAACGC 33585 0.0 21.658628 4 GTACTTT 16875 0.0 21.566082 14-15 CAACGCA 33875 0.0 21.431244 5 AGTACTT 16190 0.0 21.294395 12-13 AACGCAG 34505 0.0 21.065823 6 ATGGGAG 4845 0.0 20.9309 5 ATGGGAT 4865 0.0 19.48117 5 ACTTTTT 18820 0.0 19.477177 16-17 CATGGGG 5290 0.0 19.349333 4 ACGCAGA 37485 0.0 19.266068 7 >>END_MODULE