FastQCFastQC Report
Thu 26 May 2016
SRR938687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938687_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270644
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245631.0817635877750982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204650.9012861549410651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158120.6963663172210175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92940.4093111910101276No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86840.38244656582009334No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51650.22746850673201086No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50070.22051012840410034No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41990.18492551011959601No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37310.16431461735084849No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35700.15722411791544602No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34730.15295220210653895No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32840.14462857233454474No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28910.12732070725309647No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.11393243502724339No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25620.11283142579814362No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25210.11102577066242No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23460.10331870605872168No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1151.2906134E-424.7590072
GTACATG180450.023.3042771
GAGTACT171550.022.76786812-13
TACATGG181600.022.6298562
GGTATCA256350.021.9591731
ACATGGG181200.021.867953
GTACTTT182400.021.30968914-15
AGTACTT175050.021.0926312-13
GTATCAA328450.020.720241
ATGGGAG46700.019.6119235
CATGGGA133500.019.3729174
ACTTTTT203200.019.302716-17
CATGGGG48550.019.2555874
TACTTTT196850.018.69667614-15
TAGGACA9550.017.8887044
TACCTGG16300.017.7591442
TATCAAC386100.017.6004182
ATCAACG389150.017.4380863
ATGGGAT49100.017.3968565
TCAACGC391750.017.322354
Produced by FastQC (version 0.11.5)