##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938687_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2270644 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.797657404683427 31.0 31.0 34.0 30.0 34.0 2 32.015964193418256 33.0 31.0 34.0 30.0 34.0 3 32.07033070794012 33.0 31.0 34.0 30.0 34.0 4 35.773854906361365 37.0 35.0 37.0 35.0 37.0 5 35.69415945432221 37.0 35.0 37.0 33.0 37.0 6 35.74785259159956 37.0 35.0 37.0 33.0 37.0 7 35.674398540678325 37.0 35.0 37.0 33.0 37.0 8 35.64986453182445 37.0 35.0 37.0 33.0 37.0 9 37.23682840639044 39.0 37.0 39.0 33.0 39.0 10-11 37.15358968645019 39.0 37.0 39.0 33.0 39.0 12-13 37.065342035123074 39.0 37.0 39.0 33.0 39.0 14-15 38.143435738935736 40.0 37.0 41.0 33.0 41.0 16-17 38.083712374110604 40.0 37.0 41.0 33.0 41.0 18-19 38.16687160118451 40.0 37.5 41.0 33.0 41.0 20-21 38.265864441982096 40.0 38.0 41.0 33.0 41.0 22-23 38.20322956835153 40.0 37.5 41.0 33.0 41.0 24-25 38.11001746641041 40.0 37.0 41.0 33.0 41.0 26-27 37.897252937933025 40.0 37.0 41.0 33.0 41.0 28-29 37.69695998139734 40.0 37.0 41.0 32.0 41.0 30-31 37.3808106863075 39.0 36.0 41.0 32.0 41.0 32-33 37.05208984763794 39.0 35.0 41.0 31.0 41.0 34-35 36.92985690403251 39.0 35.0 41.0 31.0 41.0 36-37 36.77841903001968 39.0 35.0 41.0 31.0 41.0 38-39 36.64067154516516 39.0 35.0 41.0 30.0 41.0 40-41 36.42680622766052 39.0 35.0 41.0 30.0 41.0 42-43 36.16720014233847 39.0 35.0 41.0 30.0 41.0 44-45 35.79400271464836 39.0 35.0 40.5 28.5 41.0 46-47 35.34503933685774 38.0 35.0 40.0 26.5 41.0 48-49 34.94065296012937 38.0 34.0 40.0 24.5 41.0 50-51 34.65348244815127 38.0 34.0 40.0 24.0 41.0 52-53 34.419484736488855 38.0 34.0 40.0 23.5 41.0 54-55 34.16481337453163 38.0 33.5 40.0 23.0 41.0 56-57 33.89169724536299 37.5 33.0 40.0 22.5 41.0 58-59 33.57321821474436 37.0 33.0 40.0 22.0 41.0 60-61 33.19008836259669 36.5 33.0 40.0 20.5 41.0 62-63 32.80936399541275 36.0 32.0 39.0 19.5 41.0 64-65 32.43028651783371 35.5 31.5 39.0 18.0 40.0 66-67 32.01681769577265 35.0 31.0 39.0 16.0 40.0 68-69 31.55692415896107 35.0 31.0 38.0 12.5 40.0 70-71 31.053912017912097 35.0 30.5 37.5 8.0 39.5 72-73 30.689632544775844 35.0 30.0 37.0 7.5 39.0 74-75 30.26357280137265 34.0 29.5 36.0 4.5 39.0 76-77 28.29615540789309 32.0 27.0 34.5 2.0 36.5 78-79 29.379579097383825 34.0 29.0 35.0 2.0 37.0 80-81 29.438873509013302 34.0 29.0 35.0 2.0 37.0 82-83 29.11094671819977 34.0 29.0 35.0 2.0 37.0 84-85 28.694579159040345 34.0 29.0 35.0 2.0 36.0 86-87 28.119512570002165 34.0 27.5 35.0 2.0 36.0 88-89 27.5541725166957 33.0 26.5 35.0 2.0 35.0 90-91 27.06104787892774 33.0 25.5 35.0 2.0 35.0 92-93 26.71679884649465 33.0 25.0 35.0 2.0 35.0 94-95 26.264172190796973 33.0 24.5 35.0 2.0 35.0 96-97 25.63363521538383 32.0 23.5 34.0 2.0 35.0 98-99 24.772047049207185 32.0 10.5 34.0 2.0 35.0 100-101 21.54417623370286 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12118924241453044 1101 2 0.22508777800387492 1101 3 0.16908957482682752 1101 4 0.1516131729503556 1101 5 0.053318338366608486 1101 6 0.15032555890731203 1101 7 0.13298593200427433 1101 8 0.10140652228222535 1101 9 0.21197554264566065 1101 10-11 0.1817932760820966 1101 12-13 0.1666343707968494 1101 14-15 0.2954822903820613 1101 16-17 0.211640945442646 1101 18-19 0.22461499652877137 1101 20-21 0.25729283877257103 1101 22-23 0.2623330898024605 1101 24-25 0.30719730876944595 1101 26-27 0.36732695866906084 1101 28-29 0.31199321662085566 1101 30-31 0.2462129786058469 1101 32-33 0.22502720685660194 1101 34-35 0.34413689207085696 1101 36-37 0.3302137666215401 1101 38-39 0.46183339778571764 1101 40-41 0.5217887175444815 1101 42-43 0.43788075142571614 1101 44-45 0.47311637374019 1101 46-47 0.3933621841708401 1101 48-49 0.3005175017716084 1101 50-51 0.3268084787616843 1101 52-53 0.31859360255260327 1101 54-55 0.30400367956725205 1101 56-57 0.33465079916223317 1101 58-59 0.36657937120551054 1101 60-61 0.5869810002817815 1101 62-63 0.4223315518749615 1101 64-65 0.5086828713823976 1101 66-67 0.36396136135731894 1101 68-69 0.35611269309256244 1101 70-71 0.31699047248206114 1101 72-73 0.546423185623798 1101 74-75 0.6242637722317248 1101 76-77 0.49965270192172895 1101 78-79 0.5338764627607944 1101 80-81 0.6173659192186882 1101 82-83 0.6436421960853203 1101 84-85 0.7794707018398732 1101 86-87 0.6568688367007667 1101 88-89 0.8404693090769797 1101 90-91 0.7930477698173881 1101 92-93 0.8139668329059333 1101 94-95 0.8778513874072402 1101 96-97 0.7709989727294655 1101 98-99 0.8350766485164058 1101 100-101 1.2879787102984324 1102 1 0.1953199766570286 1102 2 0.16120670978070706 1102 3 0.21569951016975608 1102 4 0.20710595411552646 1102 5 0.13174482152298594 1102 6 0.13098151772642552 1102 7 0.18128235828739037 1102 8 0.10128055537230551 1102 9 0.3106880459508048 1102 10-11 0.2762014148120713 1102 12-13 0.2462820001530801 1102 14-15 0.29405228866882993 1102 16-17 0.4332792282918305 1102 18-19 0.3375881027915639 1102 20-21 0.306950638570342 1102 22-23 0.26585709333191687 1102 24-25 0.3292216128567418 1102 26-27 0.2596225768625757 1102 28-29 0.2134201483858149 1102 30-31 0.2711079814232633 1102 32-33 0.3540174216440519 1102 34-35 0.29104441433540984 1102 36-37 0.32750032846762167 1102 38-39 0.19993086171135133 1102 40-41 0.26803204164485805 1102 42-43 0.24116193194956992 1102 44-45 0.19305687616198242 1102 46-47 0.1535292091631888 1102 48-49 0.02904851171526701 1102 50-51 -0.024415786901734293 1102 52-53 0.08348191090217227 1102 54-55 0.08814373258822883 1102 56-57 -0.05646813756612801 1102 58-59 -0.13287249448128335 1102 60-61 -0.019361413826509022 1102 62-63 -0.11935121551506 1102 64-65 0.07772481974983236 1102 66-67 -0.12714856358739723 1102 68-69 0.022155004902259634 1102 70-71 0.07602468656044437 1102 72-73 0.17939195434676591 1102 74-75 0.24401066985093323 1102 76-77 0.01499065866318361 1102 78-79 0.10295178443690745 1102 80-81 0.14989182314515404 1102 82-83 0.16721117930854845 1102 84-85 0.33364875289806406 1102 86-87 0.20764082966167408 1102 88-89 0.3884138796753902 1102 90-91 0.5514881964807792 1102 92-93 0.5281384073635706 1102 94-95 0.6141100513459428 1102 96-97 0.8538421211495795 1102 98-99 0.7206448216694064 1102 100-101 1.2767486414424596 1103 1 0.08106502980070829 1103 2 0.1288854553414751 1103 3 0.004636313841842821 1103 4 0.0823535292243136 1103 5 0.10216726168194157 1103 6 0.04861121285082248 1103 7 0.10170660788417507 1103 8 0.14882729999803246 1103 9 0.11671494104351154 1103 10-11 0.07225781841255952 1103 12-13 0.13958737359659068 1103 14-15 0.17587790385324098 1103 16-17 0.1702933820079764 1103 18-19 0.1582586315484278 1103 20-21 0.19028229287577858 1103 22-23 0.049179847159372514 1103 24-25 0.174439659896251 1103 26-27 0.11229677926726112 1103 28-29 0.2222296654419651 1103 30-31 0.05581461286620737 1103 32-33 0.1455841698495277 1103 34-35 -0.05011374324608653 1103 36-37 -0.14076457714329393 1103 38-39 0.11085947498754223 1103 40-41 0.10934395253836016 1103 42-43 0.2108509140380832 1103 44-45 0.16789590955051636 1103 46-47 0.08080555531157785 1103 48-49 0.23422526444922198 1103 50-51 0.13756332729530385 1103 52-53 0.19560749175616365 1103 54-55 0.07568990993074465 1103 56-57 0.052801172314282496 1103 58-59 -0.11631075890763753 1103 60-61 -0.039355017219257604 1103 62-63 0.10561755945641238 1103 64-65 0.17044149014947862 1103 66-67 0.08063866999733449 1103 68-69 0.14884294142913745 1103 70-71 0.1422391567814998 1103 72-73 0.20488282548838654 1103 74-75 0.13514872800137567 1103 76-77 0.11981256596715184 1103 78-79 -0.07233726103084237 1103 80-81 0.08956721977881799 1103 82-83 0.013904260507313637 1103 84-85 -0.06997784955474984 1103 86-87 0.10795352008055659 1103 88-89 0.2764314889287576 1103 90-91 0.39825091658422096 1103 92-93 0.285978114645431 1103 94-95 0.23099905064857396 1103 96-97 0.3073001524616572 1103 98-99 0.5251619609579734 1103 100-101 1.1268332937907672 1104 1 0.026576887093799684 1104 2 0.08554712906184392 1104 3 0.008040810810726384 1104 4 0.020746982606873132 1104 5 0.07605617975010404 1104 6 0.08164561442192309 1104 7 0.03365339489756991 1104 8 0.006884931998804689 1104 9 -0.014383663788024137 1104 10-11 0.0449324948550327 1104 12-13 -0.06961582562749413 1104 14-15 0.04179281156930159 1104 16-17 0.07040125097294947 1104 18-19 0.09634810493540868 1104 20-21 0.0433998303594052 1104 22-23 -0.022644336892774675 1104 24-25 0.042486475382474964 1104 26-27 0.15800139087777865 1104 28-29 0.16118220902098557 1104 30-31 0.008853109627374067 1104 32-33 -0.0536451485520999 1104 34-35 0.04869199744426567 1104 36-37 0.041118925954165775 1104 38-39 0.127747987485769 1104 40-41 0.15223317034126183 1104 42-43 0.2074226238964414 1104 44-45 0.07290013703457277 1104 46-47 0.12359989660401283 1104 48-49 0.12652502367416218 1104 50-51 0.14484936941060056 1104 52-53 0.08126382198407356 1104 54-55 0.066728078128925 1104 56-57 0.12124060476911325 1104 58-59 0.08223290146565176 1104 60-61 0.041099473845761736 1104 62-63 0.12387511729356504 1104 64-65 0.13078873620327158 1104 66-67 0.07390609064759701 1104 68-69 0.11001189112148069 1104 70-71 -0.06998666392499686 1104 72-73 -0.30639750784508024 1104 74-75 -0.14676482755163534 1104 76-77 -0.08023302609583993 1104 78-79 0.013556290091305101 1104 80-81 -0.057047396771988446 1104 82-83 -0.06277679132073288 1104 84-85 0.015346442504569069 1104 86-87 0.18077622757380496 1104 88-89 0.37112969869490087 1104 90-91 0.2522180974071979 1104 92-93 0.24555578484164897 1104 94-95 0.371897480852045 1104 96-97 0.40174660874007273 1104 98-99 0.7581467819010221 1104 100-101 1.0611048021278577 1105 1 -0.04291044431656843 1105 2 -0.07482574805797881 1105 3 0.027834977027005436 1105 4 -0.011631017690362455 1105 5 -5.330755324308711E-4 1105 6 -0.04705076241623374 1105 7 0.03911401766558953 1105 8 0.009126420668955859 1105 9 -0.007225958936814436 1105 10-11 0.02207540099279015 1105 12-13 -0.0034621468620699147 1105 14-15 0.02557946883795381 1105 16-17 -0.02125704854686461 1105 18-19 -0.033417275930410995 1105 20-21 -0.02652192252730856 1105 22-23 -0.014684410894226119 1105 24-25 -0.03677908425844123 1105 26-27 0.0512781863452858 1105 28-29 0.12107972576217918 1105 30-31 0.06073365791300489 1105 32-33 -0.08133744805692089 1105 34-35 0.06740986671336202 1105 36-37 -0.020837579073813117 1105 38-39 -0.011605808597103362 1105 40-41 -0.01583462385018919 1105 42-43 0.10688036576414817 1105 44-45 -0.04738927754898015 1105 46-47 0.013508940100166456 1105 48-49 0.06567595833078599 1105 50-51 0.22023805245519412 1105 52-53 0.05974123384125818 1105 54-55 0.2456468495913029 1105 56-57 0.20125299879018144 1105 58-59 0.03895809676807005 1105 60-61 0.049286465879134767 1105 62-63 -0.09285527372124847 1105 64-65 -0.09925231702045778 1105 66-67 -0.11354007479445372 1105 68-69 0.03702454965871027 1105 70-71 0.017009981726236134 1105 72-73 -0.13116855944859296 1105 74-75 3.3533025137444383E-4 1105 76-77 0.11179457110005231 1105 78-79 -0.05946140568514835 1105 80-81 0.127578084215763 1105 82-83 0.02224022089234623 1105 84-85 0.0858744932412101 1105 86-87 0.04325443258526107 1105 88-89 0.2608700195123994 1105 90-91 0.30958952422156827 1105 92-93 0.20780822471743932 1105 94-95 0.2144989322612929 1105 96-97 0.24111020098781566 1105 98-99 0.2293577849849413 1105 100-101 0.5600405286425882 1106 1 0.006294548446277304 1106 2 -0.07228759079274383 1106 3 0.003697845468636274 1106 4 0.03849861895207596 1106 5 -0.01000625820967116 1106 6 -0.054157367180842186 1106 7 -0.06775989487173462 1106 8 -0.07071608754633729 1106 9 -0.17672299781877854 1106 10-11 -0.11843071490808654 1106 12-13 -0.007673988075389104 1106 14-15 0.022274014204690218 1106 16-17 -0.054599098268361956 1106 18-19 -0.15325455823910517 1106 20-21 -0.10745545162600934 1106 22-23 -0.08447401809333144 1106 24-25 -0.036631847971875686 1106 26-27 -0.034631732924076175 1106 28-29 0.0027776271346766634 1106 30-31 0.03529436863630764 1106 32-33 0.08741830520097693 1106 34-35 0.09149271716075447 1106 36-37 -0.005956407591241941 1106 38-39 -0.17861357266097144 1106 40-41 -0.03145708095681954 1106 42-43 0.00942545295060171 1106 44-45 -0.010447398778630657 1106 46-47 -0.008368016301993464 1106 48-49 -0.1433258522828993 1106 50-51 -0.03609953718471104 1106 52-53 -0.07910778673314667 1106 54-55 -0.09119963969686751 1106 56-57 -0.1553150058224304 1106 58-59 -0.1531780916928085 1106 60-61 -0.16299694708939683 1106 62-63 -0.06184083337520718 1106 64-65 -0.1871832015591366 1106 66-67 -0.3106321385033972 1106 68-69 -0.2668336496257986 1106 70-71 -0.21158485601250732 1106 72-73 0.01742681619338171 1106 74-75 0.2240775544576863 1106 76-77 0.17565438409426193 1106 78-79 0.027107052415232147 1106 80-81 -0.0363516561988142 1106 82-83 0.26375455198906295 1106 84-85 0.0971447438746722 1106 86-87 0.13786424955485188 1106 88-89 0.4148802549864108 1106 90-91 0.41011009283633015 1106 92-93 0.42023710713709406 1106 94-95 0.5862512187917446 1106 96-97 0.532179456421769 1106 98-99 0.5891957112069974 1106 100-101 0.9336180543387513 1107 1 -0.17489928917199293 1107 2 -0.14681694136489298 1107 3 -0.17387821833479933 1107 4 -0.06759520380514061 1107 5 -0.05306030289189323 1107 6 -0.052364133911908084 1107 7 -0.043458710941486345 1107 8 -0.04901360575112079 1107 9 -0.20184213389644867 1107 10-11 -0.12947633832909844 1107 12-13 -0.07366675569812031 1107 14-15 -0.25450302052063023 1107 16-17 -0.11690800994418993 1107 18-19 0.008039938219006615 1107 20-21 -0.1490887800056555 1107 22-23 -0.25575266276564435 1107 24-25 -0.1542245561504103 1107 26-27 -0.16023157440621816 1107 28-29 -0.1403152544227666 1107 30-31 -0.3180642284806581 1107 32-33 -0.22638661875999588 1107 34-35 -0.24397307765769227 1107 36-37 -0.19265586763978604 1107 38-39 -0.21791639130663754 1107 40-41 -0.2794594587246877 1107 42-43 -0.2196500907161152 1107 44-45 -0.25093573285501236 1107 46-47 -0.40706995319207095 1107 48-49 -0.6447543954047177 1107 50-51 -0.5434170187322351 1107 52-53 -0.48823821720819893 1107 54-55 -0.5643687088998206 1107 56-57 -0.5121187904362898 1107 58-59 -0.5363068379156246 1107 60-61 -0.6393752103281116 1107 62-63 -0.6206668104891904 1107 64-65 -0.47199530836054393 1107 66-67 -0.40312146331639553 1107 68-69 -0.35093787511745234 1107 70-71 -0.34829164751198505 1107 72-73 -0.32817986137139243 1107 74-75 -0.34771340011704055 1107 76-77 -0.5236262420512752 1107 78-79 -0.45595927740098574 1107 80-81 -0.46120894058975637 1107 82-83 -0.33768756422434265 1107 84-85 -0.3909213382573924 1107 86-87 -0.4858422942604719 1107 88-89 -0.4294816610981975 1107 90-91 -0.23514348619968573 1107 92-93 0.08002310802875456 1107 94-95 0.030723502935007474 1107 96-97 0.019399647633935757 1107 98-99 0.184007718934744 1107 100-101 0.685463103132534 1108 1 -0.12175358410016557 1108 2 -0.12049855965864964 1108 3 -0.1099427155496926 1108 4 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64-65 -1.1071176145458246 2316 66-67 -1.0099566158492834 2316 68-69 -1.0262253086466977 2316 70-71 -1.269282411575798 2316 72-73 -1.2529635165631063 2316 74-75 -1.243214099809176 2316 76-77 -1.3227272775956358 2316 78-79 -1.3362727394776712 2316 80-81 -1.3443530771696608 2316 82-83 -1.4982114936953757 2316 84-85 -1.7354189593041305 2316 86-87 -1.9927115917695701 2316 88-89 -2.1979714455930193 2316 90-91 -2.6014082497037414 2316 92-93 -2.7453757164884536 2316 94-95 -2.660977588584725 2316 96-97 -2.6913353089851526 2316 98-99 -2.976118225649639 2316 100-101 -3.470031999022517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 40.0 5 162.0 6 303.0 7 739.0 8 1457.0 9 2537.0 10 4160.0 11 6087.0 12 8105.0 13 11135.0 14 13685.0 15 18175.0 16 23908.0 17 31785.0 18 28274.0 19 18613.0 20 16358.0 21 17032.0 22 17971.0 23 19663.0 24 23085.0 25 27191.0 26 32432.0 27 38681.0 28 46531.0 29 56170.0 30 67240.0 31 80453.0 32 98293.0 33 122617.0 34 162124.0 35 232516.0 36 306826.0 37 421706.0 38 292845.0 39 21730.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57746180059846 20.52022789252454 15.646351111837948 20.25595919503905 2 15.971107756213657 22.626224102060913 39.06028421892644 22.342383922798994 3 19.994635883035826 27.318020790577474 30.533143901025433 22.154199425361263 4 12.96121276606989 18.252486959646692 36.76666179286581 32.01963848141761 5 12.494208691454935 39.581634109089755 33.122805688606405 14.801351510848903 6 29.442352037571716 37.5392619891097 17.29297943667083 15.725406536647753 7 26.754127903801738 35.43607011931417 20.357396403839616 17.452405573044473 8 26.55819230139115 34.36166127318946 19.308707133306672 19.77143929211272 9 27.113409235441576 17.170415089287445 20.46582379272136 35.250351882549616 10-11 24.88501059611282 27.87484519810239 26.04875533108669 21.191388874698102 12-13 26.96274713253156 25.64638049821989 27.136970832944314 20.253901536304237 14-15 23.137693838606666 26.25280813022237 27.59672497477582 23.01277305639514 16-17 20.479222617517706 29.84868717172687 27.968562411421853 21.703527799333568 18-19 20.343387842940285 27.45391771789401 32.006159498238276 20.196534940927435 20-21 22.008489229037426 25.86464532938261 31.918285665463774 20.20857977611618 22-23 21.28944475664173 26.340544796982705 31.893044440255714 20.476966006119852 24-25 21.138078007825094 26.541236759263008 32.14997595395844 20.17070927895346 26-27 20.874779990394778 26.430131247166273 32.44222607771952 20.252862684719435 28-29 20.269007382927487 27.101320154106062 32.356150942199655 20.273521520766796 30-31 20.55544594397008 26.708920464854906 32.1903829926664 20.545250598508616 32-33 20.4075583843174 26.932953822792122 32.05762329982155 20.601864493068927 34-35 21.079069468353516 26.667879340161743 31.918762914477227 20.334288277007513 36-37 21.140734136746435 26.416276581546107 32.07510601344092 20.367883268266535 38-39 20.900866021665077 26.760849055357205 32.120167443187974 20.218117479789743 40-41 20.8179472633643 26.882532641077635 31.935909677896735 20.36361041766133 42-43 20.59700545965311 27.17607133553293 31.287029596811895 20.93989360800206 44-45 20.90076230985744 27.04995465954138 31.004946271886848 21.04433675871433 46-47 21.20445071101141 27.420749593665295 30.39414411190681 20.98065558341648 48-49 21.299353839703624 27.53571233535508 30.091044655172716 21.073889169768574 50-51 21.083582823123407 27.88566981732855 30.0596221143522 20.971125245195836 52-53 21.294927782602645 28.132261155865912 29.53331742007994 21.0394936414515 54-55 20.75640214846537 27.86385712599597 29.777212984510122 21.60252774102854 56-57 21.191388874698102 28.145495286799694 29.28389478931968 21.379221049182522 58-59 21.515609871355114 27.58368215348116 29.43116460384021 21.469543371323514 60-61 21.123393314497147 27.733696756780446 30.071726805275805 21.071183123446605 62-63 21.209203204024938 27.730657029459483 29.890154511231177 21.169985255284406 64-65 20.877094780159286 28.063492119416345 29.763009965454735 21.296403134969637 66-67 20.87494472855776 28.026408739141694 29.420306067262924 21.67834046503762 68-69 20.847791515038345 27.958508413307502 29.56508967339006 21.628610398264094 70-71 21.061847790351308 28.00021668295826 29.23770979325495 21.70022573343548 72-73 21.04951282543631 27.74177722267339 29.381400166648756 21.827309785241546 74-75 20.70399410916022 28.542276992782668 29.014125508005655 21.739603390051457 76-77 20.857876300579818 27.840337166095342 29.370826501710006 21.93096003161483 78-79 20.759285884371987 27.927800722204243 29.467611719066824 21.84530167435695 80-81 20.727604311288847 28.203854986482906 29.23307864048231 21.835462061745933 82-83 20.699281789659672 28.05959454674533 29.180510022707214 22.060613640887784 84-85 20.58114349937727 28.028061642423914 29.11647532594277 22.274319532256047 86-87 20.67824252678727 27.952564303004014 29.066834373526014 22.302358796682704 88-89 20.656514149179404 27.857883309885928 28.887271978658553 22.598330562276114 90-91 20.62148401967624 27.74862451758763 29.082511282046752 22.547380180689373 92-93 20.737134486956123 27.937888986560637 29.011240863825417 22.31373566265782 94-95 20.463009715087374 27.714962740738475 29.077307820448254 22.744719723725897 96-97 20.26584093892326 27.462655410239435 29.216739659924603 23.054763990912708 98-99 20.43242864733541 27.652968402527833 28.76884297624393 23.14575997389283 100-101 20.474224588473785 27.150622755857068 29.404333505238405 22.97081915043074 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9461.0 1 5995.0 2 3339.5 3 4114.5 4 3292.0 5 2896.5 6 5155.5 7 10905.0 8 15739.5 9 15707.5 10 11598.0 11 7313.5 12 6083.0 13 6015.5 14 6123.0 15 6201.5 16 6233.5 17 6311.5 18 6418.5 19 6691.5 20 7140.0 21 7763.5 22 8803.5 23 9988.0 24 11161.5 25 13041.0 26 15914.0 27 18911.0 28 21519.5 29 24525.0 30 28184.5 31 32405.0 32 37482.5 33 41595.5 34 45092.5 35 48148.5 36 51797.0 37 56673.0 38 59221.0 39 59603.0 40 60116.0 41 62295.0 42 64919.5 43 67721.0 44 70633.0 45 73508.5 46 75266.0 47 81719.5 48 101691.5 49 101620.5 50 83592.5 51 75037.0 52 68765.0 53 66254.5 54 64280.0 55 59806.5 56 55068.5 57 50483.5 58 46210.0 59 41333.5 60 35074.5 61 29418.5 62 24327.0 63 18601.5 64 13485.0 65 10179.5 66 7416.5 67 5569.0 68 4112.0 69 2794.0 70 2068.0 71 1653.5 72 1419.5 73 1236.0 74 994.5 75 793.0 76 567.0 77 323.0 78 190.5 79 118.5 80 64.0 81 35.0 82 23.0 83 10.0 84 6.5 85 4.0 86 1.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35998597754645817 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.202018458199524E-4 16-17 0.002202018458199524 18-19 1.9818166123795715E-4 20-21 8.808073832798096E-5 22-23 0.0 24-25 0.0 26-27 1.9818166123795715E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007861205895772301 36-37 0.018188672464728068 38-39 0.011538576720965506 40-41 0.008455750879486173 42-43 0.0034571689793732527 44-45 0.0019818166123795715 46-47 0.0017836349511416143 48-49 0.0 50-51 4.404036916399048E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.945449837138714E-4 60-61 6.38585352877862E-4 62-63 0.0 64-65 0.0 66-67 0.0014092918132476953 68-69 6.606055374598572E-4 70-71 0.0020038367969615668 72-73 0.0 74-75 0.0 76-77 0.09197831099899412 78-79 0.0346817907166425 80-81 2.202018458199524E-5 82-83 0.0 84-85 0.0 86-87 0.1407309996635316 88-89 0.31125530906650273 90-91 0.26353756907731907 92-93 0.0 94-95 2.202018458199524E-5 96-97 2.202018458199524E-5 98-99 1.5414129207396668E-4 100-101 4.844440608038953E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2270644.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.31325524625522 #Duplication Level Percentage of deduplicated Percentage of total 1 70.69529020779359 32.74129020101631 2 14.867578320895912 13.771318993386858 3 5.854099945174991 8.13367274993934 4 2.830992551215436 5.244499224987509 5 1.6231870521300318 3.7587538128857365 6 0.9816098309505701 2.7276927991828313 7 0.6623389253448552 2.1472550196318654 8 0.4326301418561343 1.60292081496054 9 0.32986054955154454 1.3749224244345626 >10 1.550614678764622 12.797386632168664 >50 0.110278027823062 3.5238552994189853 >100 0.05448710208576719 4.671997226523044 >500 0.003991513369793013 1.2795391443598447 >1k 0.002375900815352966 2.3080648478237933 >5k 3.801441304564745E-4 1.2390019921478248 >10k+ 2.8510809784235586E-4 2.6778288171322795 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24563 1.0817635877750982 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20465 0.9012861549410651 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15812 0.6963663172210175 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9294 0.4093111910101276 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8684 0.38244656582009334 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5165 0.22746850673201086 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5007 0.22051012840410034 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4199 0.18492551011959601 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3731 0.16431461735084849 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3570 0.15722411791544602 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3473 0.15295220210653895 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3284 0.14462857233454474 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2891 0.12732070725309647 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2587 0.11393243502724339 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2562 0.11283142579814362 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2521 0.11102577066242 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2346 0.10331870605872168 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.808073832798096E-5 0.0 12-13 0.0 0.0 0.0 8.808073832798096E-5 0.0 14-15 0.0 0.0 0.0 8.808073832798096E-5 0.0 16-17 0.0 0.0 0.0 8.808073832798096E-5 0.0 18-19 0.0 0.0 0.0 1.101009229099762E-4 0.0 20-21 0.0 0.0 0.0 1.7616147665596192E-4 0.0 22-23 0.0 0.0 0.0 6.826257220418525E-4 0.0 24-25 0.0 0.0 0.0 0.0015414129207396668 0.0 26-27 0.0 0.0 0.0 0.0025983817806754382 0.0 28-29 0.0 0.0 0.0 0.005879389283392729 0.0 30-31 0.0 0.0 0.0 0.014929685146592774 0.0 32-33 0.0 0.0 0.0 0.032215530043459034 0.0 34-35 0.0 0.0 0.0 0.05822136803479541 0.0 36-37 0.0 0.0 0.0 0.10334072624330366 0.0 38-39 0.0 0.0 0.0 0.16702310005443388 0.0 40-41 0.0 0.0 0.0 0.23801617514678655 0.0 42-43 0.0 0.0 0.0 0.31171773294272465 0.0 44-45 0.0 0.0 0.0 0.40131786400686326 0.0 46-47 0.0 0.0 0.0 0.5113967667322574 0.0 48-49 0.0 0.0 0.0 0.6397524226607076 0.0 50-51 0.0 0.0 0.0 0.7795145342026315 0.0 52-53 0.0 0.0 0.0 0.9132651353536706 0.0 54-55 0.0 0.0 0.0 1.0578056269498872 0.0 56-57 0.0 0.0 0.0 1.2409475021183418 0.0 58-59 0.0 4.404036916399048E-5 0.0 1.4444360278405597 0.0 60-61 0.0 4.404036916399048E-5 0.0 1.679501498253359 0.0 62-63 0.0 4.404036916399048E-5 0.0 1.910647375810563 0.0 64-65 0.0 4.404036916399048E-5 0.0 2.1355615411310622 0.0 66-67 0.0 4.404036916399048E-5 0.0 2.3986366863321598 0.0 68-69 0.0 4.404036916399048E-5 0.0 2.709011188015382 0.0 70-71 0.0 4.404036916399048E-5 0.0 3.053627076723608 0.0 72-73 0.0 4.404036916399048E-5 0.0 3.4090108356924294 0.0 74-75 0.0 4.404036916399048E-5 0.0 3.758228942978292 0.0 76-77 0.0 4.404036916399048E-5 0.0 4.125261379590988 0.0 78-79 0.0 4.404036916399048E-5 0.0 4.558728713087564 0.0 80-81 0.0 4.404036916399048E-5 0.0 5.029960663142262 0.0 82-83 0.0 4.404036916399048E-5 0.0 5.500950391166559 0.0 84-85 0.0 4.404036916399048E-5 0.0 5.971984159560019 0.0 86-87 0.0 4.404036916399048E-5 0.0 6.462659932600619 0.0 88-89 0.0 4.404036916399048E-5 0.0 6.989316687248198 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 115 1.2906134E-4 24.759007 2 GTACATG 18045 0.0 23.304277 1 GAGTACT 17155 0.0 22.767868 12-13 TACATGG 18160 0.0 22.629856 2 GGTATCA 25635 0.0 21.959173 1 ACATGGG 18120 0.0 21.86795 3 GTACTTT 18240 0.0 21.309689 14-15 AGTACTT 17505 0.0 21.09263 12-13 GTATCAA 32845 0.0 20.72024 1 ATGGGAG 4670 0.0 19.611923 5 CATGGGA 13350 0.0 19.372917 4 ACTTTTT 20320 0.0 19.3027 16-17 CATGGGG 4855 0.0 19.255587 4 TACTTTT 19685 0.0 18.696676 14-15 TAGGACA 955 0.0 17.888704 4 TACCTGG 1630 0.0 17.759144 2 TATCAAC 38610 0.0 17.600418 2 ATCAACG 38915 0.0 17.438086 3 ATGGGAT 4910 0.0 17.396856 5 TCAACGC 39175 0.0 17.32235 4 >>END_MODULE