FastQCFastQC Report
Thu 26 May 2016
SRR938686_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938686_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249559
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248071.1027494722298903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180360.8017571444003024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135730.6033627035343372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90160.4007896658856247No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73730.3277531285020753No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49510.22008758161044006No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43850.1949270945994304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43570.19368240619605886No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39350.17492317383095976No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36090.16043144456313438No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35130.15616394146586063No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32340.14376151058940886No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30010.1334039249470674No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29780.13238150233001222No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28340.12598024768410163No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23520.10455382588320644No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22740.10108647961667154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG185050.026.3239251
TACATGG186700.025.7675692
ACATGGG184700.025.0191123
GTATCAA292700.024.3872661
TACCTGG15950.024.0937372
CATGGGA129650.023.127274
GAGTACT152400.022.85992612-13
GGTATCA217250.022.225411
AGTACTT155250.021.90525612-13
ATGGGAT50350.021.4840015
GTACTTT162550.021.21397414-15
TATCAAC340050.020.9280282
ATCAACG340550.020.8415743
TCAACGC342850.020.701764
CAACGCA345150.020.550065
AACGCAG350050.020.3161646
CATGGGG52000.020.2548684
ATGGGAG46200.020.0249965
CGGATTA1200.004798843519.7673859
GCGCGAC1456.199578E-419.6310589
Produced by FastQC (version 0.11.5)