##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938686_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2249559 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.964928681577145 33.0 31.0 34.0 30.0 34.0 2 32.02596686728376 33.0 31.0 34.0 30.0 34.0 3 32.03333497810015 34.0 31.0 34.0 30.0 34.0 4 35.60680071071708 37.0 35.0 37.0 33.0 37.0 5 35.64623421746218 37.0 35.0 37.0 35.0 37.0 6 35.73464976913253 37.0 35.0 37.0 35.0 37.0 7 35.66805582783115 37.0 35.0 37.0 35.0 37.0 8 35.64920813368309 37.0 35.0 37.0 33.0 37.0 9 37.27639595138425 39.0 37.0 39.0 34.0 39.0 10-11 37.172457134931776 39.0 37.0 39.0 34.0 39.0 12-13 37.06774816752973 39.0 37.0 39.0 33.5 39.0 14-15 38.24617047163466 40.0 38.0 41.0 33.5 41.0 16-17 38.16265543602101 40.0 38.0 41.0 33.0 41.0 18-19 38.16404148546448 40.0 38.0 41.0 33.5 41.0 20-21 38.09288798382261 40.0 38.0 41.0 33.5 41.0 22-23 38.0996177473007 40.0 38.0 41.0 33.5 41.0 24-25 38.0326048349921 40.0 38.0 41.0 33.0 41.0 26-27 37.89069635426321 40.0 38.0 41.0 33.0 41.0 28-29 37.7191200586426 40.0 37.5 41.0 33.0 41.0 30-31 37.4491540341907 40.0 37.0 41.0 32.5 41.0 32-33 37.206590047204806 40.0 36.0 41.0 32.0 41.0 34-35 36.90382137121098 39.0 35.0 41.0 31.0 41.0 36-37 36.67667062744298 39.0 35.0 41.0 31.0 41.0 38-39 36.49565448161173 39.0 35.0 41.0 30.0 41.0 40-41 36.35826977643174 39.0 35.0 41.0 30.0 41.0 42-43 36.279977764530734 39.0 35.0 41.0 30.0 41.0 44-45 36.02747716330178 39.0 35.0 41.0 29.5 41.0 46-47 35.61562755188906 39.0 35.0 41.0 27.5 41.0 48-49 35.2482977774755 39.0 35.0 41.0 26.0 41.0 50-51 34.089656017023785 38.0 34.0 40.0 23.5 40.5 52-53 34.17138670290488 38.0 33.5 39.5 23.5 40.5 54-55 34.65752220768604 38.0 35.0 40.0 24.0 41.0 56-57 34.54742573988946 38.0 35.0 40.0 23.5 41.0 58-59 34.343971640663796 38.0 34.0 40.0 23.0 41.0 60-61 34.03632156347088 38.0 34.0 40.0 22.5 41.0 62-63 33.659341897678615 37.0 34.0 40.0 21.5 41.0 64-65 33.27699984752567 36.5 33.0 40.0 20.0 41.0 66-67 32.89208640449084 36.0 33.0 39.0 18.5 41.0 68-69 32.482693496814264 35.5 33.0 39.0 15.5 40.5 70-71 32.055783155720746 35.0 32.0 38.5 11.5 40.0 72-73 31.640457529675814 35.0 32.0 37.5 7.5 40.0 74-75 31.188135318966964 35.0 31.0 37.0 4.5 39.0 76-77 30.736738622992327 35.0 31.0 36.5 2.0 39.0 78-79 30.310157902059913 35.0 31.0 36.0 2.0 38.5 80-81 29.855151387449716 34.0 30.0 35.5 2.0 37.5 82-83 29.45475290934801 34.0 30.0 35.0 2.0 37.0 84-85 29.120848797475418 34.0 29.0 35.0 2.0 36.5 86-87 28.7166235693307 34.0 29.0 35.0 2.0 36.0 88-89 28.273945915621685 34.0 29.0 35.0 2.0 36.0 90-91 27.824324011950786 34.0 27.5 35.0 2.0 35.0 92-93 27.382526753021367 34.0 26.5 35.0 2.0 35.0 94-95 26.89000510766777 33.5 25.5 35.0 2.0 35.0 96-97 26.319937374392048 33.0 25.0 35.0 2.0 35.0 98-99 25.59930368574463 33.0 22.0 35.0 2.0 35.0 100-101 23.15626840638543 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13968688122098527 1101 2 0.19292768143964878 1101 3 0.25780596946550816 1101 4 0.16568074787614506 1101 5 0.11741500287534024 1101 6 0.22621230888765353 1101 7 0.24613768560234917 1101 8 0.23303656816941754 1101 9 0.24896671049699393 1101 10-11 0.24011802568976037 1101 12-13 0.18171071394987592 1101 14-15 0.386938671568295 1101 16-17 0.3795895619285474 1101 18-19 0.42689095729269866 1101 20-21 0.3845218277890581 1101 22-23 0.28310189367022076 1101 24-25 0.34320258750634736 1101 26-27 0.35672478070466695 1101 28-29 0.4375952785414938 1101 30-31 0.37547134407886773 1101 32-33 0.37475665147000115 1101 34-35 0.4203773037627059 1101 36-37 0.395388219372947 1101 38-39 0.3939129608273504 1101 40-41 0.6655077191055838 1101 42-43 0.7601543413958538 1101 44-45 0.792857783196169 1101 46-47 0.8433183631337471 1101 48-49 0.8468568989029492 1101 50-51 0.8068951692229049 1101 52-53 0.8648848699212053 1101 54-55 0.7469276147960286 1101 56-57 0.747242482467037 1101 58-59 0.8228635088297551 1101 60-61 0.6686661978976005 1101 62-63 0.5257160633040314 1101 64-65 0.5983903255855196 1101 66-67 0.5318032413185207 1101 68-69 0.6787445348353813 1101 70-71 0.5808569313397669 1101 72-73 0.3461538474772148 1101 74-75 0.3054572860158693 1101 76-77 0.2999397043411811 1101 78-79 0.3596980485407961 1101 80-81 0.2680896274729072 1101 82-83 0.2700533812114152 1101 84-85 0.3485425060488687 1101 86-87 0.2352635002447414 1101 88-89 0.3195993480016277 1101 90-91 0.18207103512393275 1101 92-93 0.1398152214100712 1101 94-95 0.12954222959963246 1101 96-97 0.060521743192957445 1101 98-99 -0.12358953902617031 1101 100-101 0.025570784213396536 1102 1 0.08285607030639497 1102 2 0.007552984533575113 1102 3 0.033168803361348864 1102 4 0.21716200173182187 1102 5 0.2100517471492438 1102 6 0.25422906157498915 1102 7 0.1800536494439271 1102 8 0.3090394097292801 1102 9 0.3575769922618335 1102 10-11 0.36740192556455753 1102 12-13 0.27119860351997005 1102 14-15 0.39044407327470054 1102 16-17 0.48641450121318996 1102 18-19 0.3912037119794789 1102 20-21 0.38008768487900113 1102 22-23 0.38506380996935974 1102 24-25 0.34335893896821545 1102 26-27 0.4493099550708308 1102 28-29 0.4950917661865404 1102 30-31 0.44160781930077775 1102 32-33 0.338269300003887 1102 34-35 0.4035462958394831 1102 36-37 0.367158330381514 1102 38-39 0.32998732811093845 1102 40-41 0.42730563923519327 1102 42-43 0.495256432430061 1102 44-45 0.4300335446442034 1102 46-47 0.42530611460464485 1102 48-49 0.3417563066795424 1102 50-51 0.2994287350521816 1102 52-53 0.37243812309391444 1102 54-55 0.22853763117864645 1102 56-57 0.4429486406093446 1102 58-59 0.21147724017718872 1102 60-61 0.27647994670540754 1102 62-63 0.035162047978296584 1102 64-65 0.09077159601309148 1102 66-67 0.27132759680089436 1102 68-69 0.27093685080323127 1102 70-71 0.18645858219488787 1102 72-73 0.05441158035591087 1102 74-75 -0.05752707033953186 1102 76-77 0.051911432442995675 1102 78-79 0.12462483279199787 1102 80-81 0.03378706315441704 1102 82-83 -0.00495541657621601 1102 84-85 -0.08142700783039203 1102 86-87 -0.1759728100693536 1102 88-89 -0.10035807109252559 1102 90-91 -0.22448989327347135 1102 92-93 -0.15156470433698743 1102 94-95 -0.45781787012537123 1102 96-97 -0.4383543418514222 1102 98-99 -0.6001118073758853 1102 100-101 -0.14498094426698316 1103 1 0.04769331033725521 1103 2 0.13857285026232802 1103 3 0.22146589567855557 1103 4 0.18085377360817745 1103 5 0.20597530113651885 1103 6 0.09937853189860135 1103 7 0.08953149621584089 1103 8 0.1667069241836785 1103 9 0.201062010859701 1103 10-11 0.2090811944813069 1103 12-13 0.17385061338791274 1103 14-15 0.3350093529300864 1103 16-17 0.35257812806438693 1103 18-19 0.353555444597923 1103 20-21 0.4096053051159174 1103 22-23 0.3831209630828809 1103 24-25 0.33427090270428295 1103 26-27 0.27148275483254025 1103 28-29 0.32202821647639013 1103 30-31 0.4387088244256958 1103 32-33 0.37383256329926695 1103 34-35 0.21341910858532032 1103 36-37 0.2728775560687211 1103 38-39 0.37975275441068135 1103 40-41 0.3604648323856807 1103 42-43 0.5717798726152026 1103 44-45 0.660441532943203 1103 46-47 0.7092401551915302 1103 48-49 0.5922300367164297 1103 50-51 0.494452182875051 1103 52-53 0.493613913025861 1103 54-55 0.47270498524077453 1103 56-57 0.46661175191393767 1103 58-59 0.558632473166071 1103 60-61 0.426845030071199 1103 62-63 0.3911906970934709 1103 64-65 0.36160426104528653 1103 66-67 0.38817690062204946 1103 68-69 0.22761652070065708 1103 70-71 0.2009940365250884 1103 72-73 0.32489281445988993 1103 74-75 0.23832497542572995 1103 76-77 0.2735194222695405 1103 78-79 0.175614586780803 1103 80-81 0.1302222494003935 1103 82-83 0.23954465598219343 1103 84-85 0.2948691144954516 1103 86-87 0.1963369914261115 1103 88-89 0.34960039876518323 1103 90-91 0.2714923757425609 1103 92-93 0.18635957141551884 1103 94-95 0.21954304686017423 1103 96-97 0.29713559926170063 1103 98-99 0.4175379302681854 1103 100-101 0.8923324087716864 1104 1 0.13225632831163736 1104 2 0.12834175104188006 1104 3 0.11650026073229469 1104 4 0.1897933003391188 1104 5 0.25517690945179083 1104 6 0.2655996829357008 1104 7 0.25318718451634226 1104 8 0.23741052367319782 1104 9 0.3128068637895751 1104 10-11 0.2667918053643419 1104 12-13 0.282081697232492 1104 14-15 0.3149847899889906 1104 16-17 0.4340557869354882 1104 18-19 0.3846415955581506 1104 20-21 0.47093943030619556 1104 22-23 0.4140129937731061 1104 24-25 0.34203629009915204 1104 26-27 0.33662009972851337 1104 28-29 0.304887781031951 1104 30-31 0.4736322141884486 1104 32-33 0.47278143086695223 1104 34-35 0.5395505799016505 1104 36-37 0.6250099960644206 1104 38-39 0.5956592768517552 1104 40-41 0.5570175639077348 1104 42-43 0.5745238875605381 1104 44-45 0.49462995645597374 1104 46-47 0.4519350826943409 1104 48-49 0.6846109122180692 1104 50-51 0.7546744409742843 1104 52-53 0.6904437944779644 1104 54-55 0.5004101761321138 1104 56-57 0.6388108462756108 1104 58-59 0.5716414142303847 1104 60-61 0.5355531500644375 1104 62-63 0.5223940890088414 1104 64-65 0.5378762293095107 1104 66-67 0.47515412128105794 1104 68-69 0.37248463423603795 1104 70-71 0.519128905907138 1104 72-73 0.3836495606197694 1104 74-75 0.3074169922776768 1104 76-77 0.30835555400993186 1104 78-79 0.18154986208562818 1104 80-81 0.2216146491113271 1104 82-83 0.32330159434036077 1104 84-85 0.3898269846309148 1104 86-87 0.3927490338019908 1104 88-89 0.4108699453309832 1104 90-91 0.26244348818624985 1104 92-93 0.3390397765295283 1104 94-95 0.4628977910826926 1104 96-97 0.4276495759211407 1104 98-99 0.3809708607539086 1104 100-101 0.6565635387438569 1105 1 -0.07625390800800957 1105 2 -0.007309820486405272 1105 3 0.1113650447488368 1105 4 0.19137558712400704 1105 5 0.15039961041270544 1105 6 0.10501725445349308 1105 7 0.16712180432143242 1105 8 0.19024105892172116 1105 9 0.16814964011247469 1105 10-11 0.2412338065503974 1105 12-13 0.22475594558066803 1105 14-15 0.32445095844087035 1105 16-17 0.33633711385220266 1105 18-19 0.24800339081075862 1105 20-21 0.251979016340087 1105 22-23 0.1563770824231554 1105 24-25 0.1644239424893712 1105 26-27 0.1507210826881007 1105 28-29 0.28403859421715083 1105 30-31 0.24587738204729703 1105 32-33 0.27534589002926424 1105 34-35 0.22014644338940315 1105 36-37 0.3468384688296666 1105 38-39 0.3574096586473203 1105 40-41 0.40621618681966964 1105 42-43 0.35261538204746756 1105 44-45 0.41767912542741215 1105 46-47 0.6404056437632661 1105 48-49 0.5400835777546575 1105 50-51 0.6882026518618929 1105 52-53 0.6336087354111655 1105 54-55 0.5785536263045046 1105 56-57 0.4979597701631562 1105 58-59 0.5626554422654593 1105 60-61 0.5723052374759376 1105 62-63 0.5301034730453296 1105 64-65 0.46332638071572063 1105 66-67 0.37982724654674627 1105 68-69 0.4222623793592888 1105 70-71 0.3727345779985356 1105 72-73 0.41745224447204166 1105 74-75 0.3377139793727224 1105 76-77 0.3643601093165003 1105 78-79 0.37761828614898363 1105 80-81 0.36703415191116306 1105 82-83 0.16399575378658326 1105 84-85 0.15377498696104652 1105 86-87 0.22399145693119138 1105 88-89 0.287879292106318 1105 90-91 0.34897856877003974 1105 92-93 0.35448216082317074 1105 94-95 0.47819585402779197 1105 96-97 0.542496569051444 1105 98-99 0.522569783854248 1105 100-101 0.9157659927315862 1106 1 0.0805588821952874 1106 2 0.11535752294146562 1106 3 0.05328938022111629 1106 4 0.15152726039419662 1106 5 0.17067108791805197 1106 6 0.1583938771671427 1106 7 0.16764541913054387 1106 8 0.09910473456641 1106 9 0.05061780571769248 1106 10-11 0.21311157093393973 1106 12-13 0.1898139552464002 1106 14-15 0.29452779417385955 1106 16-17 0.37783668686547855 1106 18-19 0.35546238586412926 1106 20-21 0.24563583861078087 1106 22-23 0.2336560283675837 1106 24-25 0.18370835503075256 1106 26-27 0.12921203647653812 1106 28-29 0.12753647241034116 1106 30-31 0.07252842778191138 1106 32-33 0.15449898318529875 1106 34-35 0.1194960817086681 1106 36-37 0.2855312331001727 1106 38-39 0.2427350983780201 1106 40-41 0.2452048153353772 1106 42-43 0.2493761170626385 1106 44-45 0.3362273049178981 1106 46-47 0.2901797163706945 1106 48-49 0.20597010940454652 1106 50-51 0.25012028535631714 1106 52-53 -0.04902417195641107 1106 54-55 -0.07338746775873517 1106 56-57 0.04488327791565183 1106 58-59 0.12881632473872884 1106 60-61 0.1352310847218945 1106 62-63 0.027338451389695706 1106 64-65 -0.04170484291666554 1106 66-67 0.07334594535664962 1106 68-69 0.1553395206557937 1106 70-71 0.1643207516505072 1106 72-73 0.1734586824546902 1106 74-75 0.08510392728107163 1106 76-77 0.059482651955004684 1106 78-79 0.1408583387438327 1106 80-81 0.0937329282714856 1106 82-83 0.0879675033820213 1106 84-85 0.21809902430834427 1106 86-87 0.3216589279810549 1106 88-89 0.3212391343509502 1106 90-91 0.33844227927376735 1106 92-93 0.49613601059530765 1106 94-95 0.34446733007460395 1106 96-97 0.34700155250799014 1106 98-99 0.3799336093636221 1106 100-101 0.941219652456347 1107 1 0.11319286430692088 1107 2 0.09961267276512586 1107 3 0.19166077662276848 1107 4 0.1774639430997027 1107 5 0.07034719893839281 1107 6 -0.01608213737562636 1107 7 0.03688219042201979 1107 8 0.026606510176037546 1107 9 0.05613303261225866 1107 10-11 0.07785735587566478 1107 12-13 0.12564331221451397 1107 14-15 0.17613992545003754 1107 16-17 0.2736064567086842 1107 18-19 0.24028089577475953 1107 20-21 0.31991689732983275 1107 22-23 0.37658204407741636 1107 24-25 0.25031455649325807 1107 26-27 0.2103162711526707 1107 28-29 0.22437416446238956 1107 30-31 0.3013986732328533 1107 32-33 0.29361257885314274 1107 34-35 0.4616530000283987 1107 36-37 0.4604896354849899 1107 38-39 0.442806289486974 1107 40-41 0.31639703577550904 1107 42-43 0.3706713728782418 1107 44-45 0.6217578370672712 1107 46-47 0.7457300108606901 1107 48-49 0.736423064919272 1107 50-51 0.7555063012217644 1107 52-53 0.7741401144485849 1107 54-55 0.6707032811761735 1107 56-57 0.8345224429178515 1107 58-59 0.8802131932191344 1107 60-61 0.6755435364414311 1107 62-63 0.6834612202069934 1107 64-65 0.7053255263990224 1107 66-67 0.5224929302659191 1107 68-69 0.5931901271417956 1107 70-71 0.7186296754921315 1107 72-73 0.6864184097388311 1107 74-75 0.7025856515619644 1107 76-77 0.6609416528786589 1107 78-79 0.8683417191488836 1107 80-81 0.7323966472594492 1107 82-83 0.6841991540265226 1107 84-85 0.9398215379269104 1107 86-87 0.9189484418252505 1107 88-89 0.837900966024268 1107 90-91 0.8633783406548439 1107 92-93 0.9278286695193181 1107 94-95 1.0056243273103185 1107 96-97 1.2368337282216508 1107 98-99 1.29870544324881 1107 100-101 1.5560621372650196 1108 1 0.19379620849386825 1108 2 0.13304270267635587 1108 3 0.03928374937809309 1108 4 0.19015391451940644 1108 5 0.17638662182904596 1108 6 0.21543849745363275 1108 7 0.23659481562285833 1108 8 0.21457265473834042 1108 9 0.23834127054013976 1108 10-11 0.3188656662290157 1108 12-13 0.4056534942396439 1108 14-15 0.32481770130218734 1108 16-17 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-1.8184179776500429 2316 66-67 -1.5058975540806472 2316 68-69 -1.6588305107704606 2316 70-71 -1.5707777023611769 2316 72-73 -1.4334890958215745 2316 74-75 -1.4672697973577016 2316 76-77 -1.5216680903660809 2316 78-79 -1.260622974150973 2316 80-81 -1.440281434585497 2316 82-83 -1.297821968004147 2316 84-85 -1.1726483507425485 2316 86-87 -1.3223626064803042 2316 88-89 -1.3378776994894146 2316 90-91 -1.7108854237497653 2316 92-93 -1.4588127784732912 2316 94-95 -1.3471154768498685 2316 96-97 -1.6090802845579617 2316 98-99 -1.8504094269212032 2316 100-101 -2.017302112871729 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9366.0 3 1793.0 4 1563.0 5 1792.0 6 2082.0 7 2534.0 8 3170.0 9 3648.0 10 4605.0 11 5962.0 12 8332.0 13 11541.0 14 12837.0 15 15722.0 16 20869.0 17 27556.0 18 23942.0 19 15020.0 20 13883.0 21 14269.0 22 15133.0 23 16367.0 24 18568.0 25 21033.0 26 24386.0 27 29235.0 28 34424.0 29 41413.0 30 50944.0 31 63207.0 32 80529.0 33 105963.0 34 146885.0 35 226007.0 36 303774.0 37 426784.0 38 381100.0 39 63292.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.72485437845113 18.890334829287422 14.531091927641576 20.853718864619868 2 15.151084767661729 21.28583557763672 40.512284416646594 23.050795238054956 3 19.646522564971576 27.118982989705643 29.915360480625992 23.319133964696793 4 12.232103026304191 16.997817556250684 37.774397112157665 32.99568230528746 5 12.736431804947854 38.98917787780193 33.14355673628211 15.130833580968106 6 29.683766777720226 36.50178159248226 18.09346223919076 15.720989390606748 7 27.154236028563943 35.09147661759281 20.54566078623912 17.208626567604124 8 26.457752155065823 33.57188654419985 19.879092642322117 20.091268658412208 9 27.70713580160083 17.014984902920638 20.722994968530504 34.554884326948034 10-11 24.798692175567545 27.369179142418314 26.415813255996923 21.416315426017217 12-13 26.84786089670811 25.343733951324715 27.26472001756697 20.543685134400206 14-15 23.38086821942199 25.784623933281225 27.63803702151125 23.196470825785536 16-17 20.7636003894756 29.5562639136236 27.85748972768132 21.822645969219483 18-19 20.55780010896813 27.384524297977496 31.67410866430287 20.383566928751502 20-21 22.214584418881184 25.902262838412582 31.445103566831893 20.43804917587434 22-23 21.432277424248667 26.333471824169365 31.431575687840912 20.80267506374106 24-25 21.25232132248724 26.44475525347828 31.75932243333625 20.543600990698227 26-27 20.932491435750233 26.42418071854741 32.071044418147224 20.57228342755513 28-29 20.452730168673142 27.068533703976577 31.90275296234085 20.575983165009433 30-31 20.67031237219805 26.775141895682186 31.7450288315623 20.809516900557465 32-33 20.4365108523186 27.004903460477735 31.59797324227352 20.960612444930142 34-35 21.152583499857265 26.82056005566657 31.407409720239794 20.61944672423637 36-37 21.16903788580932 26.534881531264798 31.57428186263901 20.721798720286877 38-39 20.98083647089109 26.86465514972162 31.58546939336386 20.569038986023426 40-41 20.893295157564243 27.009646517353026 31.51146611008536 20.58559221499737 42-43 20.632288850036336 27.236124724127304 30.894507885932914 21.23707853990345 44-45 20.824903487608154 27.229176914659732 30.674510355074137 21.271409242657978 46-47 21.193005939931396 27.45017987116205 30.194930143060322 21.16188404584623 48-49 21.26413928957461 27.559397735214496 29.829360095896806 21.347102879314082 50-51 20.985429146704558 27.958438350100316 29.937007789685627 21.1191247135095 52-53 21.241835898662504 28.180563414473063 29.44181554976822 21.135785137096214 54-55 20.684868271479694 27.863109343272814 29.791553390974997 21.6604689942725 56-57 21.17203457523634 28.17630595662414 29.213893175983635 21.437766292155885 58-59 21.43506722013317 27.628917569201523 29.364781672272244 21.57123353839306 60-61 21.108952387321864 27.67581244740201 30.086575598990116 21.12865956628601 62-63 21.160152899434156 27.755887375534975 29.896947922952428 21.18701180207844 64-65 20.860724240199417 28.08674636418209 29.754190368011496 21.298339027607 66-67 20.8847195457117 28.022196656664853 29.39287256561527 21.700211232008176 68-69 20.800217287089453 27.950270359059324 29.550508535704623 21.699003818146597 70-71 21.01578234134636 27.987499285826512 29.273435602976676 21.723282769850453 72-73 21.022087087505927 27.81964350470027 29.400318000502107 21.757951407291696 74-75 20.769432807683753 28.50917233119606 29.103337038401573 21.618057822718622 76-77 20.897230717330757 27.93176068403529 29.261236803019013 21.90977179561494 78-79 20.879004939124723 27.96597863616096 29.337950291121285 21.81706613359303 80-81 20.90621684493625 28.26158826642015 29.063555998540174 21.76863889010343 82-83 20.846503807910675 28.110604321759975 29.091875326844924 21.95101654348443 84-85 20.80877333107255 28.015626185238563 29.05131831993967 22.124282163749214 86-87 20.896503335813396 28.00048634497252 29.02667423699055 22.076336082223534 88-89 20.9512260695827 27.80579227377033 28.821052185350084 22.421929471296885 90-91 20.887555873931895 27.72837737415394 29.022331719879404 22.361735032034762 92-93 21.127489759780232 27.86080665371599 28.96363731713495 22.048066269368828 94-95 20.84715444795699 27.653447569778823 29.046446309949303 22.45295167231488 96-97 20.701642092633683 27.40742278183142 29.185318694699497 22.705616430835402 98-99 20.978405616267125 27.49941361454245 28.629871044297268 22.892309724893163 100-101 21.22398337541984 26.921793166412243 29.200287904743334 22.65393555342458 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10708.0 1 6776.0 2 3694.0 3 4473.0 4 3506.5 5 3183.0 6 5485.5 7 10687.0 8 15609.0 9 15706.5 10 11347.0 11 7448.5 12 6523.0 13 6448.5 14 6347.5 15 6207.5 16 6049.0 17 6113.5 18 6310.0 19 6460.0 20 6760.5 21 7455.5 22 8519.0 23 9693.0 24 10849.5 25 12560.5 26 15246.0 27 18277.0 28 21095.5 29 23993.5 30 27609.5 31 31676.5 32 36471.0 33 40403.0 34 43936.5 35 47423.5 36 50932.0 37 55440.5 38 57967.0 39 58055.0 40 58384.0 41 60788.5 42 63435.0 43 66141.0 44 69357.5 45 72297.0 46 74433.0 47 80270.5 48 99760.5 49 100072.5 50 81881.5 51 74142.5 52 68343.5 53 66041.0 54 64441.0 55 60197.0 56 55326.0 57 50501.5 58 46717.0 59 42097.5 60 35772.0 61 30238.0 62 25083.5 63 19270.5 64 13933.5 65 10380.5 66 7703.5 67 5870.5 68 4354.5 69 3035.5 70 2338.0 71 1852.5 72 1525.0 73 1323.0 74 1072.5 75 851.5 76 630.5 77 426.5 78 291.5 79 196.5 80 150.0 81 123.5 82 101.0 83 79.0 84 58.5 85 46.0 86 32.0 87 23.0 88 20.5 89 19.0 90 12.0 91 5.0 92 4.0 93 3.0 94 1.5 95 2.5 96 3.0 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2546721379612626 2 0.1556749567359647 3 0.18465841527161547 4 0.15349675203006455 5 0.22182125474370756 6 0.1820801321503459 7 0.2245328973367669 8 0.23097860513994076 9 0.1377158812016044 10-11 0.19130416228247402 12-13 0.19719420561985704 14-15 0.15978687378281697 16-17 0.2680303117188747 18-19 0.2163090632430623 20-21 0.18443614948529913 22-23 0.22788911071014362 24-25 0.21690918086611644 26-27 0.3219964446364821 28-29 0.29390204924609664 30-31 0.2710531264127769 32-33 0.17790153536759873 34-35 0.33957766833410463 36-37 0.38176371457694597 38-39 0.36300448221184684 40-41 0.3244635948645934 42-43 0.357225571767622 44-45 0.3674720245168053 46-47 0.37213960602944846 48-49 0.39194348759023434 50-51 0.3843864508554788 52-53 0.3845642634845319 54-55 0.3791632048770448 56-57 0.35618092257193523 58-59 0.3310871152968204 60-61 0.29879189654505617 62-63 0.3652493666536419 64-65 0.4002562279984655 66-67 0.4059240055495321 68-69 0.4091913126083824 70-71 0.40928021892290883 72-73 0.3999228293189909 74-75 0.39716673356866833 76-77 0.3806746122239959 78-79 0.3541805304950882 80-81 0.36438253008700816 82-83 0.37496238151566597 84-85 0.3755847257173517 86-87 0.3712505428841831 88-89 0.38036344012315304 90-91 0.4011452911437308 92-93 0.4003673608916236 94-95 0.40792439762637916 96-97 0.40834670262038025 98-99 0.4062351776503751 100-101 0.3796299630283091 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2249559.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.05883609306988 #Duplication Level Percentage of deduplicated Percentage of total 1 71.39358047677024 33.59698801753724 2 14.606240852641333 13.747053884406995 3 5.612990445530112 7.924223921045065 4 2.7841528742939468 5.240759750777929 5 1.5251409467217587 3.5885678915304338 6 0.9653837702654825 2.72579019670999 7 0.6573276293927984 2.165315121972932 8 0.4276371320819525 1.609928456476406 9 0.32708642249067027 1.3853075709831353 >10 1.5355707152688498 12.82205179375552 >50 0.10585506038607187 3.455677306389827 >100 0.05220351067974499 4.527779686361047 >500 0.0038893986466669644 1.2597860443283235 >1k 0.0024664479222765833 2.7006384315267757 >5k 1.8972676325204488E-4 0.7316312360204364 >10k+ 2.8459014487806726E-4 2.518500690178104 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24807 1.1027494722298903 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18036 0.8017571444003024 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13573 0.6033627035343372 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9016 0.4007896658856247 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7373 0.3277531285020753 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4951 0.22008758161044006 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4385 0.1949270945994304 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4357 0.19368240619605886 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3935 0.17492317383095976 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3609 0.16043144456313438 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3513 0.15616394146586063 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3234 0.14376151058940886 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3001 0.1334039249470674 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2978 0.13238150233001222 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2834 0.12598024768410163 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2352 0.10455382588320644 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2274 0.10108647961667154 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.4453157263268044E-5 0.0 14-15 0.0 0.0 0.0 4.4453157263268044E-5 0.0 16-17 0.0 0.0 0.0 4.4453157263268044E-5 0.0 18-19 0.0 0.0 0.0 6.667973589490207E-5 0.0 20-21 0.0 0.0 0.0 1.5558605042143817E-4 0.0 22-23 0.0 0.0 0.0 4.889847298959485E-4 0.0 24-25 0.0 0.0 0.0 0.001022422617055165 0.0 26-27 0.0 0.0 0.0 0.002022618655478696 0.0 28-29 0.0 0.0 0.0 0.005045433349380923 0.0 30-31 0.0 0.0 0.0 0.01386938506613963 0.0 32-33 0.0 0.0 0.0 0.028094395390385406 0.0 34-35 0.0 0.0 0.0 0.0504321069151776 0.0 36-37 0.0 0.0 0.0 0.08979537767180146 0.0 38-39 0.0 0.0 0.0 0.14798456052941933 0.0 40-41 0.0 0.0 0.0 0.21497546852516425 0.0 42-43 0.0 0.0 0.0 0.2826776270371215 0.0 44-45 0.0 0.0 0.0 0.3686722597629135 0.0 46-47 0.0 0.0 0.0 0.47135905304106274 0.0 48-49 0.0 0.0 0.0 0.589404412153671 0.0 50-51 0.0 0.0 0.0 0.7218748207982097 0.0 52-53 0.0 0.0 0.0 0.8511446021197933 0.0 54-55 0.0 0.0 0.0 0.9939725964066735 0.0 56-57 0.0 0.0 0.0 1.1710072952076385 0.0 58-59 0.0 0.0 0.0 1.3655120848130677 0.0 60-61 0.0 0.0 0.0 1.5896226771558335 0.0 62-63 0.0 0.0 0.0 1.8138666289703893 0.0 64-65 0.0 0.0 0.0 2.0381994870994715 0.0 66-67 0.0 0.0 0.0 2.296583463692217 0.0 68-69 0.0 0.0 0.0 2.5966422752192764 0.0 70-71 0.0 0.0 0.0 2.9315523620407378 0.0 72-73 0.0 0.0 0.0 3.270907764588526 0.0 74-75 0.0 0.0 0.0 3.6097964089850496 0.0 76-77 0.0 0.0 0.0 3.9661329176074065 0.0 78-79 0.0 0.0 0.0 4.385637362700868 0.0 80-81 0.0 0.0 0.0 4.849461605585805 0.0 82-83 0.0 0.0 0.0 5.319553743644866 0.0 84-85 0.0 0.0 0.0 5.7896014285466615 0.0 86-87 0.0 0.0 0.0 6.282453583124514 0.0 88-89 0.0 0.0 0.0 6.815646977918783 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18505 0.0 26.323925 1 TACATGG 18670 0.0 25.767569 2 ACATGGG 18470 0.0 25.019112 3 GTATCAA 29270 0.0 24.387266 1 TACCTGG 1595 0.0 24.093737 2 CATGGGA 12965 0.0 23.12727 4 GAGTACT 15240 0.0 22.859926 12-13 GGTATCA 21725 0.0 22.22541 1 AGTACTT 15525 0.0 21.905256 12-13 ATGGGAT 5035 0.0 21.484001 5 GTACTTT 16255 0.0 21.213974 14-15 TATCAAC 34005 0.0 20.928028 2 ATCAACG 34055 0.0 20.841574 3 TCAACGC 34285 0.0 20.70176 4 CAACGCA 34515 0.0 20.55006 5 AACGCAG 35005 0.0 20.316164 6 CATGGGG 5200 0.0 20.254868 4 ATGGGAG 4620 0.0 20.024996 5 CGGATTA 120 0.0047988435 19.767385 9 GCGCGAC 145 6.199578E-4 19.631058 9 >>END_MODULE