FastQCFastQC Report
Thu 26 May 2016
SRR938686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249559
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243941.0843903182801606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203480.9045328439929782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156350.6950251138111959No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91350.40607959159995355No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86640.38514215452895434No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50480.22439953786497707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49660.22075437896938913No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41980.18661435419119926No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35380.15727527039744235No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34920.15523042516333202No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34240.1522076104694298No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33370.14834018578752547No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27760.1234019645628321No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25760.11451133311017848No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.11148851841627627No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24980.11104398684364358No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23230.10326468432257166No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG179900.023.3959311
TACATGG179300.022.9497382
GGTATCA240900.022.8415071
GAGTACT173800.022.79555312-13
ACATGGG180150.022.1037853
GTATCAA313550.022.053921
AGTACTT178500.021.34473612-13
GTACTTT187050.021.10469414-15
ATGGGAG44800.020.2345855
CATGGGG51200.019.5592174
ACTTTTT209150.019.05592316-17
CATGGGA124700.019.030164
TACTTTT200800.018.91520514-15
TATCAAC369600.018.6968482
CATGGGC22750.018.567254
ATCAACG372000.018.5379453
TCAACGC373800.018.4613764
CAACGCA376450.018.3566325
AACGCAG382900.018.0789726
AGAGTAC316150.017.73184810-11
Produced by FastQC (version 0.11.5)