##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938686_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2249559 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.844874039756238 31.0 31.0 34.0 30.0 34.0 2 32.046092589703136 33.0 31.0 34.0 30.0 34.0 3 32.09673407098902 33.0 31.0 34.0 30.0 34.0 4 35.790780326277286 37.0 35.0 37.0 35.0 37.0 5 35.706091282780314 37.0 35.0 37.0 33.0 37.0 6 35.75861002089743 37.0 35.0 37.0 33.0 37.0 7 35.67148805610344 37.0 35.0 37.0 33.0 37.0 8 35.663391802571084 37.0 35.0 37.0 33.0 37.0 9 37.24546277737103 39.0 37.0 39.0 33.0 39.0 10-11 37.16008226501283 39.0 37.0 39.0 33.0 39.0 12-13 37.05735301896949 39.0 37.0 39.0 33.0 39.0 14-15 38.12940447438809 40.0 37.0 41.0 33.0 41.0 16-17 38.06127534330062 40.0 37.0 41.0 33.0 41.0 18-19 38.272618544345804 40.0 37.5 41.0 33.5 41.0 20-21 38.29571507126508 40.0 38.0 41.0 33.0 41.0 22-23 38.20236810859373 40.0 37.5 41.0 33.0 41.0 24-25 38.09515487257725 40.0 37.0 41.0 33.0 41.0 26-27 37.870353033639034 40.0 37.0 41.0 33.0 41.0 28-29 37.656598915609685 40.0 37.0 41.0 32.0 41.0 30-31 37.33702894656241 39.0 36.0 41.0 32.0 41.0 32-33 37.07054516018473 39.0 35.0 41.0 31.0 41.0 34-35 36.960990131843616 39.0 35.0 41.0 31.0 41.0 36-37 36.779837514819576 39.0 35.0 41.0 31.0 41.0 38-39 36.61482895091882 39.0 35.0 41.0 30.0 41.0 40-41 36.377618235396355 39.0 35.0 41.0 30.0 41.0 42-43 36.0921247231124 39.0 35.0 41.0 30.0 41.0 44-45 35.723840761678176 38.5 35.0 40.0 28.5 41.0 46-47 35.24620403376839 38.0 35.0 40.0 26.5 41.0 48-49 34.80462859609372 38.0 34.0 40.0 24.0 41.0 50-51 34.519187538535334 38.0 34.0 40.0 24.0 41.0 52-53 34.26429157892724 38.0 33.5 40.0 23.0 41.0 54-55 33.96363109391663 38.0 33.0 40.0 23.0 41.0 56-57 33.6739414258528 37.5 33.0 40.0 22.0 41.0 58-59 33.340054872977326 37.0 33.0 40.0 20.5 41.0 60-61 32.933200685112055 36.0 32.0 39.5 19.5 41.0 62-63 32.55010404261458 36.0 31.5 39.0 18.0 40.5 64-65 32.118506116087644 35.5 31.0 39.0 15.5 40.0 66-67 31.683898266282412 35.0 31.0 38.5 12.5 40.0 68-69 31.32722080194385 35.0 31.0 38.0 9.0 40.0 70-71 30.868618693708413 35.0 30.0 37.0 7.5 39.5 72-73 30.359099716877843 34.5 29.5 37.0 4.5 39.0 74-75 29.838306974833735 34.0 29.0 36.0 2.0 39.0 76-77 27.995647591372354 32.0 26.5 34.5 2.0 36.5 78-79 28.96024065161216 33.5 28.5 35.0 2.0 37.0 80-81 29.0039356602783 34.0 29.0 35.0 2.0 37.0 82-83 28.645971277036963 34.0 29.0 35.0 2.0 36.5 84-85 28.198717393053485 34.0 27.5 35.0 2.0 36.0 86-87 27.55057657967628 33.0 26.5 35.0 2.0 36.0 88-89 26.873745031804013 33.0 25.5 35.0 2.0 35.0 90-91 26.259798476056865 32.0 24.5 34.5 2.0 35.0 92-93 25.878058543919053 32.0 23.5 34.0 2.0 35.0 94-95 25.430615734017202 32.0 21.5 34.0 2.0 35.0 96-97 24.659896895347043 32.0 17.0 34.0 2.0 35.0 98-99 23.652545009933057 31.0 2.0 34.0 2.0 35.0 100-101 19.94160522129004 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05636753862681232 1101 2 0.11907049783997081 1101 3 0.01921888809867056 1101 4 0.0823618159827717 1101 5 -0.014299317065585626 1101 6 0.10973284087510393 1101 7 0.17627621029799911 1101 8 0.16295657493529347 1101 9 0.17637024855987704 1101 10-11 0.21089319811919438 1101 12-13 0.2073791045810367 1101 14-15 0.24967814731447646 1101 16-17 0.2801119841160542 1101 18-19 0.3235801393632869 1101 20-21 0.20182542351403043 1101 22-23 0.14245982582902883 1101 24-25 0.1148286046143383 1101 26-27 0.07553451632760044 1101 28-29 0.17845808638780625 1101 30-31 0.2638634929736554 1101 32-33 0.2859221002049779 1101 34-35 0.3794786157202594 1101 36-37 0.287076485735156 1101 38-39 0.35212532009750674 1101 40-41 0.4751640566451485 1101 42-43 0.5291401072727027 1101 44-45 0.5081472440613979 1101 46-47 0.5045318940434527 1101 48-49 0.43844813737470645 1101 50-51 0.39532894637245164 1101 52-53 0.3660815731786826 1101 54-55 0.5591585363615437 1101 56-57 0.49285746065856273 1101 58-59 0.6817483329372962 1101 60-61 0.6770668602297434 1101 62-63 0.3234411831461088 1101 64-65 0.39530013773940453 1101 66-67 0.41896260593704326 1101 68-69 0.4877566878613173 1101 70-71 0.654243185321473 1101 72-73 0.511756115482136 1101 74-75 0.3757172725435751 1101 76-77 0.4200915442820232 1101 78-79 0.4433482433551781 1101 80-81 0.6588278895167292 1101 82-83 0.638261960243824 1101 84-85 0.6802409677628631 1101 86-87 0.9132799848646833 1101 88-89 0.8684699661303661 1101 90-91 0.9107681477942862 1101 92-93 0.8629433210355337 1101 94-95 0.9847662968618316 1101 96-97 1.1078206670270418 1101 98-99 1.1231924888970362 1101 100-101 1.270576486307462 1102 1 0.27919492931695444 1102 2 0.2568517711261009 1102 3 0.22339071980385228 1102 4 0.207447814115433 1102 5 0.06619532140231854 1102 6 0.033234966830065105 1102 7 0.19866585552406946 1102 8 0.166145797094984 1102 9 0.28853730879314554 1102 10-11 0.25665405512994255 1102 12-13 0.32984981306658767 1102 14-15 0.3368613255582389 1102 16-17 0.4841649346294119 1102 18-19 0.3087606475605895 1102 20-21 0.3650278607040036 1102 22-23 0.3065601974800316 1102 24-25 0.33195443016744264 1102 26-27 0.19142146659141446 1102 28-29 0.3252565608456379 1102 30-31 0.284931345452037 1102 32-33 0.2991835820421329 1102 34-35 0.3675752380265038 1102 36-37 0.41628218554266994 1102 38-39 0.36742555160196844 1102 40-41 0.3228802436859226 1102 42-43 0.26215062328467553 1102 44-45 0.17716858241445976 1102 46-47 0.27540795282001085 1102 48-49 0.11055014999175938 1102 50-51 0.050865623006721705 1102 52-53 0.013452628129030586 1102 54-55 0.052428953119793675 1102 56-57 0.06806695616580072 1102 58-59 -0.0064209894186646466 1102 60-61 0.10633979406304661 1102 62-63 0.06953384519247408 1102 64-65 0.18570796106824616 1102 66-67 0.3159894752109942 1102 68-69 0.27922990299204287 1102 70-71 0.3196372470176243 1102 72-73 0.24676047478107677 1102 74-75 0.23422885684453476 1102 76-77 0.1205648285589227 1102 78-79 0.11484822075712842 1102 80-81 0.2538458308228364 1102 82-83 0.2301584735386264 1102 84-85 0.4334297070582416 1102 86-87 0.4751502573817099 1102 88-89 0.38733798540940256 1102 90-91 0.5047610647543763 1102 92-93 0.45456537425954124 1102 94-95 0.680203982450724 1102 96-97 0.67094252382185 1102 98-99 0.8465873417180632 1102 100-101 1.5268963109002414 1103 1 0.2069458477536692 1103 2 0.1644455815118846 1103 3 0.22359006448684937 1103 4 0.13728211362127496 1103 5 0.06631370617574817 1103 6 0.11683600250589166 1103 7 0.017454855669193137 1103 8 0.07323265333003803 1103 9 0.18071379375827945 1103 10-11 0.23944205473278402 1103 12-13 0.1821312022164321 1103 14-15 0.33471235550576495 1103 16-17 0.21947806235512957 1103 18-19 0.05899884918130738 1103 20-21 0.23139780166437163 1103 22-23 0.21872872011443434 1103 24-25 0.2180741018979191 1103 26-27 0.311157735867134 1103 28-29 0.19404966626918707 1103 30-31 0.4356680042518448 1103 32-33 0.4434241628149138 1103 34-35 0.27071797187461755 1103 36-37 0.44850470690921185 1103 38-39 0.38269203190808554 1103 40-41 0.5238258897994612 1103 42-43 0.49396929981929105 1103 44-45 0.767401552191977 1103 46-47 0.5854402985952021 1103 48-49 0.5200911034856333 1103 50-51 0.5704509516963157 1103 52-53 0.45072349630949304 1103 54-55 0.4965371147952098 1103 56-57 0.3053234081359477 1103 58-59 0.49690695931690954 1103 60-61 0.5509707581733636 1103 62-63 0.47703304323110984 1103 64-65 0.3363266169809904 1103 66-67 0.5783336655737052 1103 68-69 0.4087875258257938 1103 70-71 0.4727730114395641 1103 72-73 0.6452264372492849 1103 74-75 0.7307002268236857 1103 76-77 0.7055787483169595 1103 78-79 0.49781398846328884 1103 80-81 0.8112061176958711 1103 82-83 0.73466387341184 1103 84-85 0.8829025129301264 1103 86-87 0.9477864762484245 1103 88-89 0.9479664558017511 1103 90-91 0.9500678722607319 1103 92-93 0.983744547704692 1103 94-95 1.0697971970937736 1103 96-97 1.295863950702067 1103 98-99 1.6249579662550282 1103 100-101 1.867908519807358 1104 1 0.09865364485377981 1104 2 0.11867855658761073 1104 3 0.11726135928826409 1104 4 0.025598379746710975 1104 5 -0.08336263190872728 1104 6 0.029733276365384143 1104 7 0.06154629592627714 1104 8 -0.011552545864262243 1104 9 0.09707563394155727 1104 10-11 0.05744411509841996 1104 12-13 -0.09021453943832114 1104 14-15 0.007171301951636622 1104 16-17 0.11163848642143392 1104 18-19 0.1255273252795277 1104 20-21 0.18272781381131153 1104 22-23 0.1640165947088903 1104 24-25 0.12116907091556328 1104 26-27 0.09427965108462644 1104 28-29 0.1376431207643236 1104 30-31 -0.03828374629015485 1104 32-33 -0.04673259410805741 1104 34-35 -0.013012443119919226 1104 36-37 0.19523022469139306 1104 38-39 0.12786823929772595 1104 40-41 0.1709196594997806 1104 42-43 0.2576962936843117 1104 44-45 0.19423224087687885 1104 46-47 0.1296278379958551 1104 48-49 0.18634281405039133 1104 50-51 0.09184665721779339 1104 52-53 0.28227419052970504 1104 54-55 0.36643367367707214 1104 56-57 0.11757643333698553 1104 58-59 0.31629169416014946 1104 60-61 0.3542229453898713 1104 62-63 0.293395075612672 1104 64-65 0.17117370402375087 1104 66-67 0.16576447419034324 1104 68-69 0.218418197597245 1104 70-71 0.2551490051047054 1104 72-73 0.34287162383538217 1104 74-75 0.21268740335396785 1104 76-77 0.18393741338453395 1104 78-79 0.2857620026705874 1104 80-81 0.42749953528893414 1104 82-83 0.5437142985544483 1104 84-85 0.5443094812712239 1104 86-87 0.5503206106642189 1104 88-89 0.6941636204926169 1104 90-91 0.727475732401885 1104 92-93 0.9766416678055556 1104 94-95 0.9911435076554547 1104 96-97 0.9777506075281828 1104 98-99 1.3019612490017707 1104 100-101 1.8367410689716799 1105 1 0.0792463595121049 1105 2 0.09354302572121043 1105 3 0.11938944317308398 1105 4 0.08424270371927634 1105 5 -0.06212115342835034 1105 6 -0.06805886940442463 1105 7 0.05333309918216145 1105 8 0.08738367459577745 1105 9 -0.006628439069295666 1105 10-11 0.0368074742696507 1105 12-13 0.04256897329082676 1105 14-15 0.11507512620496385 1105 16-17 0.10373492457055278 1105 18-19 0.06444003447988678 1105 20-21 0.07490402230580173 1105 22-23 0.049678662335246315 1105 24-25 0.018980372064355322 1105 26-27 0.0299097056118498 1105 28-29 0.15047722468618474 1105 30-31 0.016002072956538882 1105 32-33 0.07255424850819026 1105 34-35 0.022766131195076866 1105 36-37 0.002739658059546457 1105 38-39 0.0355025762329646 1105 40-41 -0.11331205236133002 1105 42-43 0.029651506833829444 1105 44-45 -0.15997170880482514 1105 46-47 -0.23246261737736518 1105 48-49 -0.14705786924559305 1105 50-51 -0.21593698671258466 1105 52-53 -0.22747930860698773 1105 54-55 -0.18245583914653452 1105 56-57 -0.05169938837628507 1105 58-59 -0.12317730823820483 1105 60-61 -0.13396369622610393 1105 62-63 -0.22091110606428543 1105 64-65 -0.30973389357684056 1105 66-67 -0.3140974496394726 1105 68-69 -0.10629434051671183 1105 70-71 -0.19250564178031482 1105 72-73 0.03178039699896118 1105 74-75 0.052320144882305186 1105 76-77 0.07911156989185386 1105 78-79 -0.20884825096762327 1105 80-81 0.040917747068707655 1105 82-83 0.19567287045534698 1105 84-85 0.24692747053371988 1105 86-87 0.23884890027255778 1105 88-89 0.03237020469494212 1105 90-91 -0.1255106699030577 1105 92-93 0.13000015678727905 1105 94-95 0.4029264273550588 1105 96-97 0.5184452917793116 1105 98-99 0.6759516555681309 1105 100-101 1.3176093062044885 1106 1 0.20692341315053753 1106 2 0.13097091017881723 1106 3 0.06622029735802926 1106 4 0.10341371500324925 1106 5 -0.08139572084449043 1106 6 0.010545339787711328 1106 7 0.04531078064787408 1106 8 0.07756589692495908 1106 9 0.12809006692200597 1106 10-11 0.11687733090275287 1106 12-13 0.05328703296978432 1106 14-15 0.056661810344905916 1106 16-17 0.2565764829590691 1106 18-19 0.0611273321534469 1106 20-21 0.02097917196993393 1106 22-23 0.15792253265088618 1106 24-25 0.13813792834383065 1106 26-27 0.15931585495000178 1106 28-29 0.22825626652942788 1106 30-31 0.19337877734827202 1106 32-33 0.23765061769893236 1106 34-35 0.2446725193686774 1106 36-37 0.08891896185603798 1106 38-39 0.12597399000024723 1106 40-41 0.432625302012859 1106 42-43 0.274968873561825 1106 44-45 0.2458379725267008 1106 46-47 0.292425312280308 1106 48-49 0.3771888510514785 1106 50-51 0.4275374819605986 1106 52-53 0.40501018810291356 1106 54-55 0.38099161742005805 1106 56-57 0.30341646686974144 1106 58-59 0.5278476422262273 1106 60-61 0.45770955208909925 1106 62-63 0.4663592199260407 1106 64-65 0.45567646218223246 1106 66-67 0.48096131846552836 1106 68-69 0.4970430110862587 1106 70-71 0.5695788848186609 1106 72-73 0.5959376141685624 1106 74-75 0.7735503188055581 1106 76-77 0.6739409101153164 1106 78-79 0.5746311913169713 1106 80-81 0.47130505429568714 1106 82-83 0.7145663950612295 1106 84-85 0.7251502358179103 1106 86-87 0.7152355496993152 1106 88-89 0.6950353749025737 1106 90-91 0.6904669838504489 1106 92-93 0.6973466475835437 1106 94-95 0.9506178548763415 1106 96-97 1.1614464651723857 1106 98-99 1.5680138957206324 1106 100-101 1.9801864172363537 1107 1 0.036893925248577375 1107 2 -0.047037983985951826 1107 3 -0.12310359273312699 1107 4 -0.06156792869442285 1107 5 -0.06162837850139624 1107 6 -0.08680086018538447 1107 7 0.07198549452186143 1107 8 -0.029565329240469396 1107 9 -0.019657326349680204 1107 10-11 -0.13435309154229458 1107 12-13 0.005392255762942 1107 14-15 -0.019681900995990986 1107 16-17 -0.11872014666154485 1107 18-19 -0.020167245651606436 1107 20-21 -0.011836261328831199 1107 22-23 0.051646044389919155 1107 24-25 -0.0492841976972187 1107 26-27 -0.02820158159209285 1107 28-29 0.04148241861942381 1107 30-31 0.07414784139359654 1107 32-33 -0.06022470838524896 1107 34-35 -0.04456428526758316 1107 36-37 0.03544087882531244 1107 38-39 -0.08605325046342216 1107 40-41 -0.16467310377312572 1107 42-43 -0.03247029166268334 1107 44-45 0.05718431254788214 1107 46-47 -0.052965771991956956 1107 48-49 -0.13199180097237928 1107 50-51 -0.2675922903723489 1107 52-53 -0.22885660616311299 1107 54-55 -0.17834708886910278 1107 56-57 -0.1335532816328069 1107 58-59 -0.11933483486868823 1107 60-61 -0.08843573557076922 1107 62-63 -0.08930739227748319 1107 64-65 0.037443980141169675 1107 66-67 -0.09879384191731333 1107 68-69 -0.1422509242759915 1107 70-71 -0.03156806452283334 1107 72-73 -0.013932052194661537 1107 74-75 -9.83937955929548E-4 1107 76-77 0.03628364778858284 1107 78-79 0.02628959403523723 1107 80-81 0.14823635626491694 1107 82-83 0.14694428924934755 1107 84-85 0.1943855065942124 1107 86-87 0.18934249308321327 1107 88-89 0.2971104206682824 1107 90-91 0.4023580664554771 1107 92-93 0.6237088652782852 1107 94-95 0.6422130803481956 1107 96-97 0.8552668990769163 1107 98-99 1.1179721591688647 1107 100-101 1.749241292743612 1108 1 0.05301063395590333 1108 2 0.021301613089185878 1108 3 -0.08947759762286722 1108 4 0.017790424087436918 1108 5 0.030562632860707595 1108 6 -0.04590904706024901 1108 7 0.02705639003587379 1108 8 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-1.3432321998340413 2316 58-59 -1.217099487658409 2316 60-61 -1.398164122883312 2316 62-63 -1.3116771685980844 2316 64-65 -1.4418154411091777 2316 66-67 -1.3844111847082061 2316 68-69 -1.375812739241482 2316 70-71 -1.1356206920894252 2316 72-73 -1.2336344473565966 2316 74-75 -1.5596607588671745 2316 76-77 -1.3943547040720148 2316 78-79 -1.242447596998442 2316 80-81 -1.2777739348683177 2316 82-83 -1.3517758574774845 2316 84-85 -1.582455103261438 2316 86-87 -1.7466389679639 2316 88-89 -2.0592291099371813 2316 90-91 -2.6160186789444246 2316 92-93 -2.6034625866484795 2316 94-95 -2.4200078212138365 2316 96-97 -2.650974055003619 2316 98-99 -2.5535409779685274 2316 100-101 -2.4772301350885257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 19.0 4 43.0 5 126.0 6 316.0 7 718.0 8 1515.0 9 2535.0 10 4111.0 11 6182.0 12 8347.0 13 11329.0 14 13900.0 15 18375.0 16 24399.0 17 32506.0 18 29629.0 19 19926.0 20 17830.0 21 17960.0 22 19616.0 23 21606.0 24 24938.0 25 29066.0 26 34684.0 27 40805.0 28 49249.0 29 58467.0 30 70089.0 31 83638.0 32 101546.0 33 127635.0 34 168702.0 35 239349.0 36 309215.0 37 400854.0 38 245847.0 39 14484.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.562658630784384 20.500855609398382 15.656426827241749 20.280058932575486 2 15.946147667164986 22.74734736897321 39.00360026120675 22.302904702655056 3 20.019079295097395 27.344115002095965 30.523226996935843 22.113578705870797 4 12.987212160250076 18.226994713186006 36.81774961225733 31.968043514306583 5 12.450267758211933 39.56863449536781 33.163112860397575 14.817984886022684 6 29.404892517190675 37.56412604535456 17.240148372332044 15.790833065122722 7 26.677050924203368 35.42969977671179 20.380483463647764 17.51276583543708 8 26.515685963337702 34.38318354841993 19.337345675307915 19.763784812934446 9 27.153233144807494 17.151806198459344 20.49619503200405 35.19876562472911 10-11 24.908593195377403 27.83005469071938 26.04743863130507 21.213913482598144 12-13 26.91920949839502 25.65109428114577 27.166191240149733 20.263504980309474 14-15 23.149537309312628 26.253412335484423 27.564113677391884 23.032936677811072 16-17 20.48708038277746 29.889035612493505 27.928649207570288 21.69523479715875 18-19 20.35576750820939 27.424464083849326 32.025632579541146 20.194135828400146 20-21 21.959837461475782 25.84324305341625 31.996515761533708 20.200403723574265 22-23 21.24498623952517 26.328560397837975 31.907653900164433 20.518799462472423 24-25 21.114263843225395 26.498523258894675 32.24081015386202 20.146402744017912 26-27 20.837491614903932 26.444581460902672 32.466047306833836 20.25187961735956 28-29 20.31924923951761 27.052079985454924 32.409307779880415 20.21936299514705 30-31 20.578944584249623 26.69956644835721 32.21360275502887 20.50788621236429 32-33 20.388107180118414 26.92245457887524 32.07030800259073 20.619130238415618 34-35 21.017909247454185 26.683869585309488 31.97920179139153 20.3190193758448 36-37 21.151577409050866 26.40030801919162 32.03808625293186 20.410028318825653 38-39 20.872021450314787 26.747961913331803 32.14041669130406 20.239599945049353 40-41 20.80726896384323 26.925058648999133 31.915258253426803 20.352414133730832 42-43 20.639282682498582 27.142362085916545 31.29472759277499 20.923627638809887 44-45 20.879010718959826 27.046969244338225 31.02110184078753 21.05291819591442 46-47 21.212195974698002 27.4078919497294 30.41060960980785 20.96930246576475 48-49 21.309530001329176 27.53399952078602 30.062120001760377 21.09435047612443 50-51 21.096255353273843 27.892301509896612 30.015829776338286 20.995613360491262 52-53 21.30944331755691 28.159141413939352 29.518830135150935 21.012585133352804 54-55 20.769759761802202 27.848569430719532 29.7673899639885 21.61428084348977 56-57 21.221568928601673 28.122063985038732 29.253939080474844 21.402428005884754 58-59 21.558737028528345 27.59482777015157 29.375915737889148 21.47051946343094 60-61 21.155072920551785 27.72116774238576 30.074865425829618 21.048893911232835 62-63 21.22093378625546 27.72035000036674 29.89939500978089 21.15932120359691 64-65 20.92175219602404 28.034602937515558 29.734454461396208 21.30919040506419 66-67 20.862717361417822 27.978899735735464 29.432343587911802 21.726039314934916 68-69 20.899645596179685 27.90408314542559 29.54787674437471 21.648394514020012 70-71 21.07349183572225 27.940046835908955 29.32365320089831 21.662808127470488 72-73 21.05432664802301 27.755284480202562 29.38329245865523 21.807096413119194 74-75 20.75624097735211 28.53044890139017 28.977072055602292 21.736238065655424 76-77 20.874405968767118 27.87021414146266 29.284991995075398 21.970387894694824 78-79 20.811605982916063 27.91290794354676 29.37611966010185 21.89936641343532 80-81 20.79263047696524 28.164976317327806 29.171428761944135 21.870964443762823 82-83 20.729354529398716 28.037480601563793 29.15966707815875 22.073497790878747 84-85 20.608461480672435 28.012045916555202 29.064118789504967 22.3153738132674 86-87 20.708017959089233 27.924759603618515 28.994228706954473 22.37299373033778 88-89 20.654029051157433 27.80506603336603 28.888223315635674 22.652681599840857 90-91 20.68682807992879 27.68749065809405 29.049746904064484 22.57593435791268 92-93 20.743388512505053 27.91542132528254 28.98045631782883 22.360733844383578 94-95 20.479300411037855 27.69752343747048 29.010564115170617 22.81261203632105 96-97 20.296324943905635 27.43457324384907 29.187451309868717 23.081650502376576 98-99 20.442028859878757 27.653642336120104 28.633123203258947 23.27120560074219 100-101 20.595335496904564 26.942996543931674 29.4577313441992 23.00393661496456 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9094.0 1 5798.5 2 3245.0 3 4009.5 4 3220.5 5 2843.5 6 5160.0 7 10787.0 8 15382.5 9 15384.5 10 11364.5 11 7203.5 12 6087.5 13 5981.5 14 6013.5 15 6064.0 16 6082.5 17 6112.0 18 6405.5 19 6767.5 20 7035.0 21 7723.5 22 8815.0 23 10013.0 24 11212.0 25 13072.0 26 15955.0 27 18778.5 28 21330.0 29 24449.0 30 28187.5 31 32413.0 32 37490.5 33 41336.5 34 44691.5 35 47817.0 36 51507.5 37 56382.0 38 58526.5 39 58551.0 40 59287.5 41 61773.5 42 64129.5 43 66658.0 44 69912.5 45 72683.0 46 74390.0 47 80733.5 48 100071.0 49 100362.5 50 82778.5 51 74257.0 52 67965.0 53 65649.5 54 63777.5 55 59178.5 56 54371.5 57 49717.5 58 45735.5 59 41365.5 60 35079.0 61 29239.5 62 24321.5 63 18648.0 64 13567.0 65 10306.0 66 7568.5 67 5625.0 68 4070.0 69 2790.0 70 2135.0 71 1694.5 72 1414.5 73 1233.5 74 980.5 75 754.5 76 566.5 77 358.0 78 223.5 79 142.0 80 98.0 81 69.5 82 45.0 83 37.0 84 26.5 85 12.5 86 4.0 87 2.0 88 1.0 89 0.5 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22048766002580952 2 0.0 3 0.0 4 0.0 5 0.022893375990583043 6 0.003689612052851248 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0023782439135848405 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.889847298959485E-4 26-27 0.0014225010324245774 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.005978949651909552 36-37 0.015491925306248915 38-39 0.011913446146555836 40-41 0.007112505162122888 42-43 0.007934888571493345 44-45 0.00386742468190432 46-47 6.001176230541186E-4 48-49 0.0018670326050572578 50-51 4.4453157263268044E-5 52-53 0.0 54-55 0.0 56-57 7.779302521071908E-4 58-59 3.1117210084287633E-4 60-61 0.0024893768067430107 62-63 8.223834093704589E-4 64-65 0.001733673133267454 66-67 0.004823167563064583 68-69 2.8894552221124227E-4 70-71 1.3335947178980413E-4 72-73 0.0 74-75 5.112113085275825E-4 76-77 0.05581093894403304 78-79 0.13613779411875837 80-81 0.001066875774318433 82-83 9.77969459791897E-4 84-85 0.0 86-87 0.09908608753982447 88-89 0.3355546576017788 90-91 0.36958354948681055 92-93 3.7785183673777836E-4 94-95 4.223049940010465E-4 96-97 6.667973589490207E-5 98-99 0.0 100-101 0.0016669933973725516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2249559.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.03152110126072 #Duplication Level Percentage of deduplicated Percentage of total 1 70.40712527319623 32.40947072692239 2 15.062340788552012 13.866849156852238 3 5.854983696704846 8.085414167472198 4 2.8558326045013334 5.25833275183086 5 1.5907376325057878 3.6612036448629848 6 1.0261715523973076 2.834174248061408 7 0.6354595871496507 2.04758199764143 8 0.4604730898853022 1.6957021402894412 9 0.32479424739854057 1.3455695927424614 >10 1.610890861959637 13.109838702838026 >50 0.10788137994818228 3.405949196701677 >100 0.05595582139328058 4.712943969481672 >500 0.00445078108899828 1.418277927131765 >1k 0.0023221466551295094 2.44203550887558 >5k 2.902683318911887E-4 1.017566756725887 >10k+ 2.902683318911887E-4 2.68908951156996 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24394 1.0843903182801606 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20348 0.9045328439929782 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15635 0.6950251138111959 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9135 0.40607959159995355 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8664 0.38514215452895434 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5048 0.22439953786497707 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4966 0.22075437896938913 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4198 0.18661435419119926 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3538 0.15727527039744235 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3492 0.15523042516333202 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3424 0.1522076104694298 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3337 0.14834018578752547 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2776 0.1234019645628321 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2576 0.11451133311017848 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2508 0.11148851841627627 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2498 0.11104398684364358 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2323 0.10326468432257166 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.4453157263268044E-5 0.0 14-15 0.0 0.0 0.0 4.4453157263268044E-5 0.0 16-17 0.0 0.0 0.0 4.4453157263268044E-5 0.0 18-19 0.0 0.0 0.0 6.667973589490207E-5 0.0 20-21 0.0 0.0 0.0 1.5558605042143817E-4 0.0 22-23 0.0 0.0 0.0 4.4453157263268044E-4 0.0 24-25 0.0 0.0 0.0 9.112897238969949E-4 0.0 26-27 0.0 0.0 0.0 0.001867032605057258 0.0 28-29 0.0 0.0 0.0 0.004934300456222753 0.0 30-31 0.0 0.0 0.0 0.01386938506613963 0.0 32-33 0.0 0.0 0.0 0.02827220801943848 0.0 34-35 0.0 0.0 0.0 0.05096554480233681 0.0 36-37 0.0 0.0 0.0 0.09043994845211883 0.0 38-39 0.0 0.0 0.0 0.14865135788836836 0.0 40-41 0.0 0.0 0.0 0.21559781272685002 0.0 42-43 0.0 0.0 0.0 0.2830332522952277 0.0 44-45 0.0 0.0 0.0 0.36925015080733603 0.0 46-47 0.0 0.0 0.0 0.47178135803506377 0.0 48-49 0.0 0.0 0.0 0.5896044513613558 0.0 50-51 0.0 0.0 0.0 0.7214080626469455 0.0 52-53 0.0 0.0 0.0 0.8498110074018952 0.0 54-55 0.0 0.0 0.0 0.9921055638016162 0.0 56-57 0.0 0.0 0.0 1.1690513562880547 0.0 58-59 0.0 0.0 0.0 1.3635116927362207 0.0 60-61 0.0 0.0 0.0 1.5876889648148813 0.0 62-63 0.0 0.0 0.0 1.8106437750688023 0.0 64-65 0.0 0.0 0.0 2.0352211255628325 0.0 66-67 0.0 0.0 0.0 2.2924715466453645 0.0 68-69 0.0 0.0 0.0 2.5895742232144165 0.0 70-71 0.0 0.0 0.0 2.9232618482111383 0.0 72-73 0.0 0.0 0.0 3.261639281299135 0.0 74-75 0.0 0.0 0.0 3.600150073858921 0.0 76-77 0.0 0.0 0.0 3.9550418548702213 0.0 78-79 0.0 0.0 0.0 4.370501062652725 0.0 80-81 0.0 0.0 0.0 4.829879989811337 0.0 82-83 0.0 0.0 0.0 5.29594911713807 0.0 84-85 0.0 0.0 0.0 5.761173634476802 0.0 86-87 0.0 0.0 0.0 6.246846604156637 0.0 88-89 0.0 0.0 0.0 6.7727719077383615 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 17990 0.0 23.395931 1 TACATGG 17930 0.0 22.949738 2 GGTATCA 24090 0.0 22.841507 1 GAGTACT 17380 0.0 22.795553 12-13 ACATGGG 18015 0.0 22.103785 3 GTATCAA 31355 0.0 22.05392 1 AGTACTT 17850 0.0 21.344736 12-13 GTACTTT 18705 0.0 21.104694 14-15 ATGGGAG 4480 0.0 20.234585 5 CATGGGG 5120 0.0 19.559217 4 ACTTTTT 20915 0.0 19.055923 16-17 CATGGGA 12470 0.0 19.03016 4 TACTTTT 20080 0.0 18.915205 14-15 TATCAAC 36960 0.0 18.696848 2 CATGGGC 2275 0.0 18.56725 4 ATCAACG 37200 0.0 18.537945 3 TCAACGC 37380 0.0 18.461376 4 CAACGCA 37645 0.0 18.356632 5 AACGCAG 38290 0.0 18.078972 6 AGAGTAC 31615 0.0 17.731848 10-11 >>END_MODULE