FastQCFastQC Report
Thu 26 May 2016
SRR938685_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938685_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464192
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149190.4306631964971918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118980.343456713715637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78820.22752780446349394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80550.048.3842281
GTATCAA137600.038.488611
ATCAACG166050.031.6520523
TCAACGC168350.031.191474
TATCAAC170750.030.836322
CAACGCA172550.030.3498485
AACGCAG177550.029.5001136
ACGCAGA199750.026.0085017
GTACATG137350.025.6137281
CGCAGAG201800.025.6031158
TACATGG139000.024.7531812
ACATGGG137800.023.9713653
GCAGAGT218100.023.0382219
GAGTACT129450.022.73723812-13
TGGTATC42150.022.150212
GTGGTAT44600.021.6866681
GTACTTT139050.021.08368314-15
CAGAGTA214800.020.3168610-11
TACCTGG28300.019.425882
CATGGGG61100.019.3137824
Produced by FastQC (version 0.11.5)