##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938685_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3464192 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.745859351906592 33.0 31.0 34.0 30.0 34.0 2 31.83844140278599 33.0 31.0 34.0 30.0 34.0 3 31.853745693079368 34.0 31.0 34.0 30.0 34.0 4 35.402641077630804 37.0 35.0 37.0 33.0 37.0 5 35.42937689365947 37.0 35.0 37.0 33.0 37.0 6 35.51109580531333 37.0 35.0 37.0 33.0 37.0 7 35.42965978791014 37.0 35.0 37.0 33.0 37.0 8 35.40279753547148 37.0 35.0 37.0 33.0 37.0 9 37.0082584914462 39.0 37.0 39.0 33.0 39.0 10-11 36.90712480717004 39.0 37.0 39.0 33.0 39.0 12-13 36.79433241575525 39.0 37.0 39.0 33.0 39.0 14-15 37.99115291531186 40.0 38.0 41.0 33.0 41.0 16-17 37.91863817017071 40.0 38.0 41.0 33.0 41.0 18-19 37.85194686091302 40.0 38.0 41.0 33.0 41.0 20-21 37.75132974731193 40.0 38.0 41.0 32.0 41.0 22-23 37.672803066342745 40.0 37.5 41.0 32.0 41.0 24-25 37.694721886084835 40.0 38.0 41.0 32.0 41.0 26-27 37.608040922674036 40.0 37.0 41.0 32.0 41.0 28-29 37.48697618376811 40.0 37.0 41.0 32.0 41.0 30-31 37.28776335145396 40.0 37.0 41.0 31.5 41.0 32-33 37.088390308620305 40.0 36.5 41.0 31.0 41.0 34-35 36.77766287203481 39.5 36.0 41.0 30.0 41.0 36-37 36.54278414706806 39.0 36.0 41.0 30.0 41.0 38-39 36.38278608691435 39.0 35.0 41.0 30.0 41.0 40-41 36.209839841440655 39.0 35.0 41.0 30.0 41.0 42-43 35.96019042824416 39.0 35.0 41.0 29.0 41.0 44-45 35.93122985100133 39.0 35.0 41.0 28.5 41.0 46-47 35.75622886953148 39.0 35.0 41.0 27.5 41.0 48-49 35.54050381734038 39.0 35.0 41.0 26.5 41.0 50-51 34.42276568388819 38.0 34.0 40.0 24.5 40.5 52-53 34.53565362427949 38.0 34.0 39.5 25.0 40.5 54-55 35.05693896296741 39.0 35.0 40.0 26.0 41.0 56-57 34.972571670392405 39.0 35.0 41.0 25.5 41.0 58-59 34.761934817700634 38.0 34.5 40.0 25.0 41.0 60-61 34.488177906998224 38.0 34.0 40.0 24.0 41.0 62-63 34.142776728310665 38.0 34.0 40.0 23.0 41.0 64-65 33.757154626533406 37.0 33.5 40.0 22.0 41.0 66-67 33.370560147936374 36.5 33.0 39.5 21.5 41.0 68-69 32.97374871254249 36.0 33.0 39.0 20.5 41.0 70-71 32.5770642908938 35.5 32.0 39.0 19.5 40.5 72-73 32.16565565649941 35.0 32.0 38.0 18.0 40.0 74-75 31.714692776843776 35.0 31.5 37.0 16.0 39.5 76-77 31.279042703175804 35.0 31.0 37.0 12.0 39.0 78-79 30.866411272816286 35.0 31.0 36.0 7.5 39.0 80-81 30.438555657423144 35.0 31.0 36.0 7.0 37.5 82-83 29.996282394278378 34.0 30.0 35.0 2.0 37.0 84-85 29.6081861224782 34.0 30.0 35.0 2.0 37.0 86-87 29.24148055304094 34.0 29.0 35.0 2.0 36.0 88-89 28.90199475086831 34.0 29.0 35.0 2.0 36.0 90-91 28.519417947965934 34.0 29.0 35.0 2.0 35.5 92-93 28.103446489109146 34.0 29.0 35.0 2.0 35.0 94-95 27.640418169662652 34.0 27.0 35.0 2.0 35.0 96-97 27.100553751062296 34.0 26.5 35.0 2.0 35.0 98-99 26.44748313603865 33.5 25.0 35.0 2.0 35.0 100-101 24.25935788201116 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3049490309741181 1101 2 0.08702933840136495 1101 3 0.26189704260094615 1101 4 0.19446519048016597 1101 5 0.044703990113440284 1101 6 0.016398725161877792 1101 7 0.15507038613327495 1101 8 0.22157251062327532 1101 9 0.3200747316784387 1101 10-11 0.33057901505444676 1101 12-13 0.26413106814612064 1101 14-15 0.4465647038210605 1101 16-17 0.45147001771336903 1101 18-19 0.511552096684845 1101 20-21 0.4441501375052397 1101 22-23 0.39225573294111626 1101 24-25 0.42838044926165253 1101 26-27 0.26507159032012595 1101 28-29 0.3700632727292614 1101 30-31 0.3451874641231214 1101 32-33 0.38698227159152765 1101 34-35 0.3716031724693849 1101 36-37 0.37224201143492763 1101 38-39 0.3997682710904087 1101 40-41 0.33364472741030227 1101 42-43 0.33187278033202716 1101 44-45 0.42550423790012815 1101 46-47 0.4876411321044358 1101 48-49 0.60075107844402 1101 50-51 0.561954866861015 1101 52-53 0.4976781804761714 1101 54-55 0.5924076124238482 1101 56-57 0.6690307442815779 1101 58-59 0.5509772691331349 1101 60-61 0.39723538279319115 1101 62-63 0.4546267182829311 1101 64-65 0.4645686194248029 1101 66-67 0.3565016368779368 1101 68-69 0.3270390702783246 1101 70-71 0.2639479088462977 1101 72-73 0.30514654787766915 1101 74-75 0.17638750076356402 1101 76-77 0.10727618662897598 1101 78-79 0.07073967681734317 1101 80-81 -0.02002214874487862 1101 82-83 -0.010944376453075222 1101 84-85 0.0320604774772022 1101 86-87 0.012008039788419467 1101 88-89 -0.10014419461812452 1101 90-91 -0.14865472003496194 1101 92-93 -0.23272570423138816 1101 94-95 -0.3310986374532021 1101 96-97 -0.571709993813986 1101 98-99 -0.7091044095217391 1101 100-101 -0.4897367922677667 1102 1 0.23496782916193482 1102 2 0.22508416023714872 1102 3 0.28225870373892903 1102 4 0.2321959662320765 1102 5 0.170360379345027 1102 6 0.22205655433413796 1102 7 0.24823132166341821 1102 8 0.18591963039806814 1102 9 0.3929124667502393 1102 10-11 0.2865394567596482 1102 12-13 0.32213996130804645 1102 14-15 0.3204122312535844 1102 16-17 0.3715068686480194 1102 18-19 0.5255683399967879 1102 20-21 0.4523296542981612 1102 22-23 0.3994737327429476 1102 24-25 0.384069628557441 1102 26-27 0.4538791844449932 1102 28-29 0.4168430058698789 1102 30-31 0.3951274680099388 1102 32-33 0.3816889613640342 1102 34-35 0.5076578267083036 1102 36-37 0.4775394988488202 1102 38-39 0.507200071518028 1102 40-41 0.4643248625032186 1102 42-43 0.5189190366433323 1102 44-45 0.6004302435428954 1102 46-47 0.5727646770617909 1102 48-49 0.6074729524588918 1102 50-51 0.4973804894676732 1102 52-53 0.6039165112045595 1102 54-55 0.4924020768028541 1102 56-57 0.48374731157167616 1102 58-59 0.5093970717814571 1102 60-61 0.5042239939321931 1102 62-63 0.4700429133534598 1102 64-65 0.4848308176793381 1102 66-67 0.4901333956525562 1102 68-69 0.30088449213722157 1102 70-71 0.19470043152302452 1102 72-73 0.20683217843286172 1102 74-75 0.20038704976327537 1102 76-77 0.23660136810947208 1102 78-79 0.14198231250989224 1102 80-81 0.11541153107700808 1102 82-83 0.0584761739285895 1102 84-85 -0.03410959361053045 1102 86-87 -0.011426400417235527 1102 88-89 0.03950806140638363 1102 90-91 -0.06147509940668883 1102 92-93 -0.15714821882458452 1102 94-95 -0.15306491550285983 1102 96-97 -0.32957768618933514 1102 98-99 -0.2968521954219199 1102 100-101 -0.15718673798556893 1103 1 -2.1956175273274425E-4 1103 2 0.10293764726147003 1103 3 0.07981502378097716 1103 4 0.1603972627891963 1103 5 0.07550482685940096 1103 6 0.20145517825844905 1103 7 0.07716690150882499 1103 8 0.10381678525038751 1103 9 0.2017669106930171 1103 10-11 0.1848708302071813 1103 12-13 0.15163179212216704 1103 14-15 0.26202133680438777 1103 16-17 0.333701303358545 1103 18-19 0.4044605384158473 1103 20-21 0.3328484456158165 1103 22-23 0.4102640833659592 1103 24-25 0.26301746470874576 1103 26-27 0.2656225786751847 1103 28-29 0.18595062887096248 1103 30-31 0.11281108516168814 1103 32-33 0.15277010232081523 1103 34-35 0.2628949756978116 1103 36-37 0.24341554253012987 1103 38-39 0.2520339754083736 1103 40-41 0.26427036195400433 1103 42-43 0.28466813700521243 1103 44-45 0.26066779686414066 1103 46-47 0.19109168709595536 1103 48-49 0.11475294545047632 1103 50-51 0.12675102075957767 1103 52-53 0.10285695145444151 1103 54-55 0.13420905219926027 1103 56-57 0.11000263527292731 1103 58-59 0.10279934648443856 1103 60-61 0.18103952768378662 1103 62-63 0.0835774129592366 1103 64-65 -0.030432368589558223 1103 66-67 -0.19901851009771576 1103 68-69 -0.1584452100724576 1103 70-71 -0.2303626189577912 1103 72-73 -0.21399465470616263 1103 74-75 -0.2076225514308483 1103 76-77 -0.263989630118207 1103 78-79 -0.2574681332658493 1103 80-81 -0.239255594393871 1103 82-83 -0.21404337719578947 1103 84-85 -0.21295963202204504 1103 86-87 -0.23093528055309065 1103 88-89 -0.2500801014449614 1103 90-91 -0.32717648019958645 1103 92-93 -0.15166249005031673 1103 94-95 -0.2296937406495978 1103 96-97 -0.24159859519447835 1103 98-99 -0.16841878717490033 1103 100-101 0.04651639322682044 1104 1 -0.023546804277650324 1104 2 -0.03624009360231639 1104 3 -0.020341122398424005 1104 4 0.07127101694201343 1104 5 -0.0073058043698353 1104 6 -0.016863758618626434 1104 7 0.03606557260516752 1104 8 0.03156263242646418 1104 9 0.08980843893221646 1104 10-11 0.01666318901116881 1104 12-13 0.10272066255405576 1104 14-15 0.08535688497714489 1104 16-17 0.17597455917249505 1104 18-19 0.1538392759573739 1104 20-21 0.07616738249289057 1104 22-23 0.14476408336596336 1104 24-25 0.0948372321506028 1104 26-27 0.02359433947253109 1104 28-29 0.08739415046564858 1104 30-31 -2.686490575740663E-4 1104 32-33 0.04952176345038595 1104 34-35 0.05846889596358551 1104 36-37 0.13618713721451314 1104 38-39 0.10375324451135981 1104 40-41 -0.04247548522207012 1104 42-43 -0.040448972629341995 1104 44-45 0.06423175035251205 1104 46-47 0.04618803261090676 1104 48-49 0.04515826106509735 1104 50-51 0.031687067271214175 1104 52-53 0.1096983135806866 1104 54-55 0.019506394391946458 1104 56-57 0.008549977465619918 1104 58-59 -0.03752208208698704 1104 60-61 -0.09780266500724366 1104 62-63 -0.07965514518030403 1104 64-65 -0.1536948270613152 1104 66-67 -0.19721867621066735 1104 68-69 -0.2013073363183011 1104 70-71 -0.2626209246056348 1104 72-73 -0.281282860686229 1104 74-75 -0.3319090962813469 1104 76-77 -0.3388500952344877 1104 78-79 -0.2480860734651884 1104 80-81 -0.267791308679584 1104 82-83 -0.1886953705512724 1104 84-85 -0.10913654232104975 1104 86-87 -0.08396102806139538 1104 88-89 -0.18440153001639104 1104 90-91 -0.15945887222616406 1104 92-93 -0.14984106147888454 1104 94-95 -0.146427129353917 1104 96-97 -0.048792116789162066 1104 98-99 -0.020364800463937627 1104 100-101 0.06531456598429486 1105 1 0.05909983401318897 1105 2 0.0968096191957315 1105 3 0.04625369556925918 1105 4 0.14604468527542025 1105 5 0.13430227860879995 1105 6 0.13630728150699412 1105 7 0.1379878007964166 1105 8 0.10735973148014466 1105 9 0.11248656612755781 1105 10-11 0.17741805491471752 1105 12-13 0.1842192368753146 1105 14-15 0.2891762163544769 1105 16-17 0.08154991352598273 1105 18-19 0.15215379308617827 1105 20-21 0.12405217112051758 1105 22-23 0.21011583343828022 1105 24-25 0.22769433368372916 1105 26-27 0.16548189680960235 1105 28-29 0.24080015897839502 1105 30-31 0.2616962255240196 1105 32-33 0.23141113904441823 1105 34-35 0.2403978599703649 1105 36-37 0.3302051958119776 1105 38-39 0.3136567529207994 1105 40-41 0.2186605947812339 1105 42-43 0.2552232039068798 1105 44-45 0.4033254732336289 1105 46-47 0.4111487334663124 1105 48-49 0.28490585394082757 1105 50-51 0.40720225384917086 1105 52-53 0.48343288938960427 1105 54-55 0.5002282010781798 1105 56-57 0.29792119115213467 1105 58-59 0.32866172716132525 1105 60-61 0.47063016279556535 1105 62-63 0.3349980223059603 1105 64-65 0.3599730104526273 1105 66-67 0.20915133907921302 1105 68-69 0.3276829525366125 1105 70-71 0.30150089861020035 1105 72-73 0.2642878240528148 1105 74-75 0.34178180003668146 1105 76-77 0.38694712779125595 1105 78-79 0.28988136320574753 1105 80-81 0.18714554821532658 1105 82-83 0.4285764588631693 1105 84-85 0.35519181835501357 1105 86-87 0.35939336674830713 1105 88-89 0.3986633900216283 1105 90-91 0.38631401095083007 1105 92-93 0.4270491819195996 1105 94-95 0.45910851261475827 1105 96-97 0.5356262889300787 1105 98-99 0.5676949540713743 1105 100-101 0.7690648035974199 1106 1 0.10019221070098183 1106 2 0.11866343737150231 1106 3 0.14143938463447725 1106 4 0.1985334541852879 1106 5 0.07709011281202294 1106 6 0.09830216810640025 1106 7 0.04941705179690814 1106 8 0.2273879508779828 1106 9 0.22394444988611895 1106 10-11 0.1766479614272498 1106 12-13 0.04986459849032343 1106 14-15 0.005321865683342253 1106 16-17 0.16819820134502805 1106 18-19 0.20294043358568814 1106 20-21 0.1815794400838584 1106 22-23 0.1449903395379053 1106 24-25 0.07599116767195113 1106 26-27 -0.10881249011961103 1106 28-29 -2.5250320017278227E-4 1106 30-31 0.05928539434311375 1106 32-33 0.11695294887837804 1106 34-35 0.12152856153763025 1106 36-37 0.21975449956462256 1106 38-39 0.16925031932153445 1106 40-41 0.20747962037520296 1106 42-43 0.2513119086618971 1106 44-45 0.25684081221717037 1106 46-47 0.31004081880235645 1106 48-49 0.27752405501554733 1106 50-51 0.20698303009432806 1106 52-53 0.08513904218115442 1106 54-55 0.19849950671094518 1106 56-57 0.2715393140076756 1106 58-59 0.10579636942954806 1106 60-61 0.16953869012325384 1106 62-63 0.08916390797102025 1106 64-65 0.1903718921619486 1106 66-67 0.07585513815388367 1106 68-69 0.3454610135426961 1106 70-71 0.28426990979958333 1106 72-73 0.16157158518738868 1106 74-75 0.05954993031505751 1106 76-77 -0.029201823378546266 1106 78-79 0.10575924415648785 1106 80-81 0.03428673787450265 1106 82-83 -0.0011372194372114564 1106 84-85 -0.09977518802039853 1106 86-87 0.028539158652847618 1106 88-89 0.040050235470520335 1106 90-91 0.11932383388561618 1106 92-93 0.2157550963568866 1106 94-95 0.2549480418008052 1106 96-97 0.34996142040846223 1106 98-99 0.47273266437154504 1106 100-101 0.7638892309359591 1107 1 0.10707417773659245 1107 2 0.15176706777563354 1107 3 0.12671341108307388 1107 4 0.1386350536543688 1107 5 0.1824098806941592 1107 6 0.18178455187237574 1107 7 -0.02365175875260661 1107 8 0.050241563981799686 1107 9 0.10132278226678437 1107 10-11 0.12589152995860786 1107 12-13 0.21924747656401422 1107 14-15 0.19635297502941285 1107 16-17 0.1543444813851167 1107 18-19 0.32491138362498617 1107 20-21 0.24330702936287452 1107 22-23 0.2706825506664501 1107 24-25 0.21237137216788682 1107 26-27 0.30028327226258256 1107 28-29 0.11027417680634954 1107 30-31 0.18906600131078477 1107 32-33 0.08551069212470708 1107 34-35 0.1768043694788375 1107 36-37 0.2526430371198458 1107 38-39 0.2993068107952652 1107 40-41 0.14461740690452984 1107 42-43 0.24513002889347746 1107 44-45 0.27233921217908375 1107 46-47 0.4070042650675916 1107 48-49 0.3157083701320573 1107 50-51 0.3011390983861659 1107 52-53 0.3023109406539888 1107 54-55 0.3078778394263182 1107 56-57 0.18983499207647725 1107 58-59 0.26710178094136694 1107 60-61 0.08994965009338785 1107 62-63 0.014026214658365177 1107 64-65 0.067819927846692 1107 66-67 0.06998073112356451 1107 68-69 0.07135628470486921 1107 70-71 0.16503483536472885 1107 72-73 0.017284657937466363 1107 74-75 0.05749033574729623 1107 76-77 -0.023366661065658434 1107 78-79 0.04699241359082862 1107 80-81 0.06132961159863726 1107 82-83 0.20558717461891263 1107 84-85 0.08104932424683753 1107 86-87 0.10042795000173754 1107 88-89 0.0594830647895499 1107 90-91 0.07854886535460892 1107 92-93 0.2050628772789871 1107 94-95 0.03673009170217867 1107 96-97 0.11074704442415495 1107 98-99 0.26330289513634497 1107 100-101 0.3637981647825015 1108 1 0.0643943626328003 1108 2 0.029742353984225645 1108 3 0.030149044999593144 1108 4 0.043505049121442596 1108 5 0.10364462631438442 1108 6 0.09490968522990073 1108 7 0.13999893844297162 1108 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-1.6633291172816946 2316 86-87 -1.6968397089075609 2316 88-89 -1.7768051081635896 2316 90-91 -1.92674976945041 2316 92-93 -1.9728782250677845 2316 94-95 -1.9445835005291023 2316 96-97 -1.8940881969519445 2316 98-99 -2.1775892792906753 2316 100-101 -2.2077588781743387 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12324.0 3 3662.0 4 3627.0 5 4001.0 6 4399.0 7 5036.0 8 6082.0 9 7508.0 10 9230.0 11 11636.0 12 14823.0 13 18816.0 14 21005.0 15 19805.0 16 24276.0 17 30077.0 18 26432.0 19 19533.0 20 19446.0 21 20826.0 22 22620.0 23 25242.0 24 28802.0 25 32734.0 26 38321.0 27 45314.0 28 53444.0 29 64646.0 30 78200.0 31 96083.0 32 121118.0 33 157640.0 34 211913.0 35 303728.0 36 448915.0 37 686066.0 38 652228.0 39 114580.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83304373971846 18.836036046454478 14.184487997525203 23.146432216301857 2 15.375587566600993 20.036219730334054 40.86975892325468 23.718433779810276 3 19.288422786071806 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22-23 22.29599973772844 26.468081652530334 29.35510514619834 21.880813463542882 24-25 22.095744204488497 26.532732055183928 29.53279127780755 21.838732462520024 26-27 21.953016652941727 26.776746405166257 29.367011410356987 21.90322553153503 28-29 21.729796918433735 26.859467486815426 29.580196608075948 21.830538986674895 30-31 21.893397893756628 26.660260303975846 29.520002264467244 21.926339537800285 32-33 21.64898329327348 26.766964305698654 29.45641586962125 22.127636531406612 34-35 22.246089786621948 26.831238516719885 29.15895566306815 21.763716033590015 36-37 22.14302366107958 26.60225946173523 29.13835477179506 22.116362105390127 38-39 21.926744693529294 26.82737125560419 29.33154681416079 21.914337236705723 40-41 22.14343648257796 26.654680465627244 29.33393984070921 21.86794321108558 42-43 21.997623326536903 26.74856400855927 29.097609149989896 22.15620351491393 44-45 22.07337462292191 27.01013480944845 28.917324709518134 21.999165858111507 46-47 22.096677767256413 27.07924406201563 28.49539556506022 22.328682605667737 48-49 22.255769566475646 27.15728401061787 28.38454748492544 22.202398937981037 50-51 22.121139941758166 27.386115352001823 28.23441591663186 22.25832878960815 52-53 22.407927497521136 27.259254297581855 28.17833737766425 22.154480827232767 54-55 22.090886593777277 27.226110632459484 28.36795934500741 22.315043428755835 56-57 22.123635859765088 27.604532569855913 28.132107142903735 22.139724427475276 58-59 22.217752523335015 27.20935638074956 28.24781749562514 22.325073600290285 60-61 22.163890243808805 27.13004396604464 28.57611816144313 22.129947628703427 62-63 22.161318466535718 27.145951441563632 28.508494805593326 22.184235286307324 64-65 22.03259352233143 27.07127964281659 28.44728721546209 22.448839619389883 66-67 21.968603499552657 27.39163407677439 28.19235214194257 22.447410281730374 68-69 22.03485789555277 27.205331170020518 28.330135882360874 22.429675052065832 70-71 21.878609078796323 27.154295568725562 28.331997255142404 22.635098097335714 72-73 22.027190134317955 27.102988310544866 28.29295906660681 22.576862488530367 74-75 21.9374406164622 27.267427133598947 28.188942196229235 22.606190053709614 76-77 22.0781800087126 27.200855893436625 28.154066617329086 22.566897480521682 78-79 22.02030906510588 27.27221869231697 28.12763776679127 22.57983447578588 80-81 21.94668329785003 27.368737018060322 28.08390592404742 22.600673760042227 82-83 22.089444192543233 27.285476806469905 27.969523883520363 22.655555117466495 84-85 22.045893854606888 27.155852260084984 27.963028058001854 22.83522582730627 86-87 22.101301734316888 27.040339339572245 28.146411224531953 22.71194770157891 88-89 22.117732377541945 26.97605345611724 28.026113327188916 22.880100839151897 90-91 22.10020130503193 27.15067545348729 28.085850709497294 22.663272531983488 92-93 22.14715958907377 26.980500099537373 28.099856935869948 22.77248337551891 94-95 21.957601568187794 26.898353005521685 28.084977241760207 23.05906818453031 96-97 22.15490996365138 26.719512428536714 28.07416116004703 23.051416447764883 98-99 22.033745450369718 26.640436863100696 27.987647943182164 23.33816974334742 100-101 22.258460903252146 26.346801712897445 28.298398935542508 23.096338448307907 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4081.0 1 2494.0 2 1279.0 3 1655.0 4 1349.0 5 1204.5 6 2073.5 7 4316.0 8 6782.0 9 7505.0 10 6097.0 11 4650.0 12 4514.0 13 4847.0 14 5138.0 15 5282.5 16 5497.5 17 5828.0 18 6066.0 19 6347.0 20 6901.5 21 7777.5 22 9224.0 23 11107.5 24 13192.0 25 16190.0 26 20377.0 27 25352.5 28 30097.0 29 34998.5 30 40933.5 31 47049.5 32 53452.0 33 59039.5 34 65572.5 35 72202.5 36 79296.5 37 87240.5 38 92719.0 39 95958.5 40 99014.0 41 104676.0 42 110703.5 43 115962.5 44 120788.5 45 125533.0 46 127066.0 47 128422.5 48 135751.5 49 138412.5 50 134635.0 51 130473.0 52 123804.5 53 119901.5 54 115809.5 55 106029.5 56 96266.5 57 86221.0 58 76360.5 59 67094.5 60 56307.5 61 45266.5 62 36032.0 63 27698.5 64 20480.5 65 15438.5 66 11532.5 67 9048.5 68 6805.0 69 4759.5 70 3508.0 71 2546.0 72 2000.0 73 1595.0 74 1226.0 75 1042.5 76 783.0 77 492.0 78 301.5 79 195.5 80 130.0 81 91.5 82 67.0 83 51.5 84 39.0 85 29.5 86 27.0 87 21.5 88 19.5 89 20.0 90 15.0 91 12.0 92 7.5 93 5.0 94 6.0 95 5.0 96 5.0 97 4.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15362889816730713 2 0.08934262304167898 3 0.08276100169967485 4 0.09863771984924624 5 0.11774751514927578 6 0.03637211794265445 7 0.039518594812296776 8 0.04353107449009755 9 0.04376200857227313 10-11 0.04220320351758794 12-13 0.04631671685634053 14-15 0.04397850927431274 16-17 0.04304033956547443 18-19 0.04673528488028377 20-21 0.048828125 22-23 0.0615294995196571 24-25 0.07727631724800473 26-27 0.0788495556828259 28-29 0.06873175620750813 30-31 0.05799332138634348 32-33 0.0480487224726574 34-35 0.2803828425214307 36-37 0.44472130874963045 38-39 0.4229990716449896 40-41 0.39938606174253616 42-43 0.4281806551138043 44-45 0.43699945037688437 46-47 0.43663861587348507 48-49 0.452226666420337 50-51 0.4415603984998522 52-53 0.4485172877253917 54-55 0.4407232624519657 56-57 0.41965052745344367 58-59 0.39830355823233815 60-61 0.3688738961350872 62-63 0.4260878149940881 64-65 0.461622796888856 66-67 0.46293623448122967 68-69 0.4732272345181791 70-71 0.47023952483003256 72-73 0.4605980268992019 74-75 0.4543772400605971 76-77 0.43774998614395505 78-79 0.4129101389299439 80-81 0.4170525190289684 82-83 0.42919099172332253 84-85 0.427790953850133 86-87 0.43702831713715634 88-89 0.44834408716375995 90-91 0.45303493570795156 92-93 0.4543195065400532 94-95 0.4584185864986698 96-97 0.4595010900088679 98-99 0.4531359693689034 100-101 0.4416325654005321 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3464192.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.10487459084668 #Duplication Level Percentage of deduplicated Percentage of total 1 74.56543175082007 41.08918765441276 2 14.593632988928707 16.083606313595187 3 4.862556549191452 8.038517065022855 4 2.184281534153173 4.814582400424512 5 1.1213275202655033 3.089530618974782 6 0.6327042571219654 2.091905324508043 7 0.4092052314255401 1.5784442071735947 8 0.2763092085659326 1.2180787429057451 9 0.19604580881161224 0.9722771737762497 >10 1.0245703529291545 10.208626961891412 >50 0.08223655124212392 3.1405503319801022 >100 0.047546919365400404 4.77675909578191 >500 0.0033631005025671844 1.3081503583866443 >1k 6.305813414717472E-4 0.5850133169066825 >5k 5.2548445122645597E-5 0.22823714120964564 >10k+ 1.0509689024529119E-4 0.776533293049869 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14919 0.4306631964971918 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11898 0.343456713715637 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7882 0.22752780446349394 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.8866760271947975E-5 0.0 6 0.0 0.0 0.0 2.8866760271947975E-5 0.0 7 0.0 0.0 0.0 2.8866760271947975E-5 0.0 8 0.0 0.0 0.0 2.8866760271947975E-5 0.0 9 0.0 0.0 0.0 2.8866760271947975E-5 0.0 10-11 0.0 0.0 0.0 5.773352054389595E-5 0.0 12-13 0.0 0.0 0.0 5.773352054389595E-5 0.0 14-15 0.0 0.0 0.0 8.660028081584393E-5 0.0 16-17 0.0 0.0 0.0 1.0103366095181791E-4 0.0 18-19 0.0 0.0 0.0 1.5876718149571386E-4 0.0 20-21 0.0 0.0 0.0 3.0310098285545376E-4 0.0 22-23 0.0 0.0 0.0 5.484684451670115E-4 0.0 24-25 0.0 0.0 0.0 7.794025273425954E-4 0.0 26-27 0.0 0.0 0.0 0.001270137451965711 0.0 28-29 0.0 0.0 0.0 0.0030021430682825893 0.0 30-31 0.0 0.0 0.0 0.008169293156961278 0.0 32-33 0.0 0.0 0.0 0.018763394176766184 0.0 34-35 0.0 0.0 0.0 0.037108220329589124 0.0 36-37 0.0 0.0 0.0 0.06767811945758204 0.0 38-39 0.0 0.0 0.0 0.11107929352645582 0.0 40-41 0.0 0.0 0.0 0.15827644657109075 0.0 42-43 0.0 0.0 0.0 0.2054158660951818 0.0 44-45 0.0 0.0 0.0 0.2726754175288206 0.0 46-47 0.0 0.0 0.0 0.35598488767366243 0.0 48-49 0.0 0.0 0.0 0.4463378473248596 0.0 50-51 0.0 1.4433380135973987E-5 0.0 0.5421466246674549 0.0 52-53 0.0 2.8866760271947975E-5 0.0 0.6405245436742537 0.0 54-55 0.0 2.8866760271947975E-5 0.0 0.7532781092964824 0.0 56-57 0.0 2.8866760271947975E-5 0.0 0.8893127170780373 0.0 58-59 0.0 2.8866760271947975E-5 0.0 1.0394343038723028 0.0 60-61 0.0 2.8866760271947975E-5 0.0 1.2035995695388708 1.4433380135973987E-5 62-63 0.0 2.8866760271947975E-5 0.0 1.37267218445167 5.773352054389595E-5 64-65 0.0 2.8866760271947975E-5 0.0 1.5532337699527048 8.660028081584393E-5 66-67 0.0 2.8866760271947975E-5 0.0 1.761117744051138 8.660028081584393E-5 68-69 0.0 2.8866760271947975E-5 0.0 2.0006974209281703 8.660028081584393E-5 70-71 0.0 2.8866760271947975E-5 0.0 2.264294242351463 8.660028081584393E-5 72-73 0.0 2.8866760271947975E-5 0.0 2.527183828148093 8.660028081584393E-5 74-75 0.0 2.8866760271947975E-5 0.0 2.7958611993792495 8.660028081584393E-5 76-77 0.0 2.8866760271947975E-5 0.0 3.0923372607522905 8.660028081584393E-5 78-79 0.0 2.8866760271947975E-5 0.0 3.4321423292935265 8.660028081584393E-5 80-81 1.4433380135973987E-5 2.8866760271947975E-5 0.0 3.801203859370381 8.660028081584393E-5 82-83 2.8866760271947975E-5 2.8866760271947975E-5 0.0 4.187080277305646 8.660028081584393E-5 84-85 2.8866760271947975E-5 2.8866760271947975E-5 0.0 4.570921588641738 8.660028081584393E-5 86-87 2.8866760271947975E-5 2.8866760271947975E-5 0.0 4.973814961757316 8.660028081584393E-5 88-89 2.8866760271947975E-5 2.8866760271947975E-5 0.0 5.4158949619420635 8.660028081584393E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8055 0.0 48.384228 1 GTATCAA 13760 0.0 38.48861 1 ATCAACG 16605 0.0 31.652052 3 TCAACGC 16835 0.0 31.19147 4 TATCAAC 17075 0.0 30.83632 2 CAACGCA 17255 0.0 30.349848 5 AACGCAG 17755 0.0 29.500113 6 ACGCAGA 19975 0.0 26.008501 7 GTACATG 13735 0.0 25.613728 1 CGCAGAG 20180 0.0 25.603115 8 TACATGG 13900 0.0 24.753181 2 ACATGGG 13780 0.0 23.971365 3 GCAGAGT 21810 0.0 23.038221 9 GAGTACT 12945 0.0 22.737238 12-13 TGGTATC 4215 0.0 22.15021 2 GTGGTAT 4460 0.0 21.686668 1 GTACTTT 13905 0.0 21.083683 14-15 CAGAGTA 21480 0.0 20.31686 10-11 TACCTGG 2830 0.0 19.42588 2 CATGGGG 6110 0.0 19.313782 4 >>END_MODULE