FastQCFastQC Report
Thu 26 May 2016
SRR938685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464192
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143550.41438234370381316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128640.37134200413833873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88740.25616363065326636No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35590.10273679980786284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79800.052.817181
GTATCAA134100.037.1481971
ATCAACG171900.028.8213733
TCAACGC173700.028.5227094
TATCAAC175900.028.2738862
CAACGCA178900.027.7201795
AACGCAG184050.026.918746
ACGCAGA209200.023.4784157
CGCAGAG210600.023.2096678
GTACATG134150.023.111341
TACATGG137050.021.8148172
GAGTACT139850.021.54896412-13
GCAGAGT227200.021.2635529
GTGGTAT40000.020.9549921
ACATGGG135500.020.8735833
TGGTATC39250.020.6750472
GTACTTT149550.020.2623414-15
AGTACTT146050.019.75682412-13
CAGAGTA225900.019.74802410-11
TACCTGG27050.019.473582
Produced by FastQC (version 0.11.5)